Food microbiology最新文献

{"title":"Development of qPCR-PMAxx™ and ddPCR-PMAxx™ methods for quantitative detection of viable but not culturable Cronobacter sakazakii on stainless steel surfaces after desiccation and exposure to commercial disinfectant","authors":"Romain Delattre ,&nbsp;Brice Bargoumane ,&nbsp;Karine Le Barillec ,&nbsp;Simon Le Hello ,&nbsp;Aurélie Hanin","doi":"10.1016/j.fm.2025.104969","DOIUrl":"10.1016/j.fm.2025.104969","url":null,"abstract":"<div><div><em>Cronobacter</em> spp. is an opportunistic foodborne pathogen responsible for life-threatening infections such as meningitis and necrotizing enterocolitis in neonates, but also for various complications in elderly and immunocompromised people. Environmental monitoring in powdered milk manufacturing plants shows that <em>Cronobacter</em> can persist in production environments for long periods of time. The aim of this study is to develop a method to assess the viability of <em>Cronobacter</em> cells after implementation of stress encountered in industries to determine whether this bacterium can persist in processing environments in a dormant state known as Viable But Non-Cultivable (VBNC) state, making it undetectable by conventional enumeration methods. We developed two detection systems specific to the genus <em>Cronobacter</em> based on quantitative PCR (qPCR) and Droplet Digital PCR (ddPCR) in combination with a PMAxx™ (Propidium Monoazide) treatment and agar enumeration to assess the viability of detected cells. Despite a better sensitivity for ddPCR, qPCR-PMAxx™ was more suitable for VBNC detection, as it effectively differentiates viable from dead cells. Desiccation at 58 °C and 20 % relative humidity led to a significant reduction in culturable bacteria, with a drop from 6.82 to 2.58 log copies of genome per coupon over 48 h, while qPCR-PMAxx™ revealed that the proportion of VBNC cells represents approximately 1/100 of the total population of viable bacteria (V). Similarly, treatment of <em>Cronobacter</em> biofilms with a peracetic acid containing disinfectant induced the VBNC state in <em>Cronobacter</em>. The ability of this bacterium to enter the VBNC state may explain its long-term survival in processing plants and highlights the need for appropriate detection methods for effective environmental monitoring plans.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"135 ","pages":"Article 104969"},"PeriodicalIF":4.6,"publicationDate":"2025-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145414006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and genomic characterization of pathogenic blaNDM-positive Escherichia coli from retail meats: The first large-scale study in 22 cities of China","authors":"Mei-Jun Chu ,&nbsp;Ming Zou ,&nbsp;Han Wang ,&nbsp;Junjie Wang ,&nbsp;Zhiyuan You ,&nbsp;Yan Li ,&nbsp;Bao-Tao Liu","doi":"10.1016/j.fm.2025.104948","DOIUrl":"10.1016/j.fm.2025.104948","url":null,"abstract":"<div><div>Retail meat is a suspected reservoir of <em>bla</em>_NDM-positive carbapenem-resistant <em>Escherichia coli</em> (CREC), resulting in foodborne illnesses in humans. Abuse of antibiotics in swine and chicken farming is common in China, however, large-scale studies about <em>bla</em>_NDM-positive CREC from retail meats and their pathogenicity in China are rare. In the present study, we sampled 817 pork and chicken meats, from 152 markets in 22 cities of China, during the period of 2017–2020, and analyzed the prevalence of and genomic characteristics of pathogenic <em>bla</em>_NDM-positive CREC. A total of 203 CREC carrying 7 <em>bla</em>_NDM variants were obtained in the 185 CREC-positive meat samples. Notably, 37.2 % of the <em>bla</em>_NDM-positive isolates co-harbored <em>mcr-1</em>, conferring resistance to nearly all currently available antibiotics. We firstly revealed high prevalence rates of <em>bla</em>_NDM-positive ExPEC (extraintestinal pathogenic <em>E. coli</em>, 19.1 %), APEC (avian pathogenic <em>E. coli</em>, 29.0 %) and UPEC (uropathogeni<em>c E. coli</em>, 32.2 %) in retail meats in China, and found that chicken meat had a higher detection rate of pathogenic CREC than pork. The most common <em>bla</em>_NDM subtype in pathogenic strains was <em>bla</em>_NDM-5, followed by <em>bla</em>_NDM-9. The <em>bla</em>_NDM-13 subtype was firstly found in isolates from meat. Whole-genome sequencing showed these pathogenic strains had high genetic diversity, with ST457, ST156 and ST6751 being the main sequence types. The genetic relationship among CREC of different origins based on phylogenomic analysis indicated the wide spread of these isolates, which was confirmed by the high similarities of the <em>bla</em>_NDM/<em>mcr-1</em>-bearing plasmids from meats and from animals and humans in different geographical areas. The horizontal transmission of these multidrug-resistant pathogens was mainly mediated by transferable <em>bla</em>_NDM-5-bearing IncFIB, IncFIC and IncX3 plasmids, <em>bla</em>_NDM-9-bearing IncB/O plasmids and <em>mcr-1</em>-bearing IncI2 and IncX4 plasmids. This study highlighted retail meat could act as an important vehicle for spreading pathogenic <em>bla</em>_NDM-positive CREC between animals and humans.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"135 ","pages":"Article 104948"},"PeriodicalIF":4.6,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145414007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Persistence of vegetative and sporulated forms of Clostridium perfringens exposed to air at different relative humidities","authors":"Léa Savard ,&nbsp;Sylvie Moundanga ,&nbsp;Stéphane Guyot ,&nbsp;Narjes Mtimet ,&nbsp;Olivier Firmesse ,&nbsp;Sébastien Dupont ,&nbsp;Laurent Beney","doi":"10.1016/j.fm.2025.104968","DOIUrl":"10.1016/j.fm.2025.104968","url":null,"abstract":"<div><div><em>C. perfringens</em>, an anaerobic bacterium, is a common cause of food poisoning that can persist on surfaces in slaughterhouses. However, the mechanisms governing its survival in such environments – characterised by variations in relative air humidity (RAH) – remain poorly understood. This study aimed to evaluate the effect of air exposure on <em>C. perfringens</em> survival and to identify the mechanisms responsible for its inactivation. Vegetative cells and spores of <em>C. perfringens</em> were deposited on inert surfaces and exposed to different RAH (11 %, 43 %, 75 %, 100 %) under both aerobic and anaerobic conditions, to assess the contributions of osmotic and oxidative effects induced by dehydration to cell death. At low RAH, more than 99 % of vegetative cells were inactivated within one day, regardless of oxygen presence. Epifluorescence microscopy and flow cytometry analyses revealed that dehydration and rehydration disrupted membrane integrity, contributing to inactivation through lethal mechanical damage. At 100 % RAH, vegetative cells survived over 3 days under aerobic conditions (&gt;1 %) and over 30 days under anaerobic conditions (&gt;0.003 %). The composition of the dehydration medium had little effect on cell survival. In contrast, spores were much more resistant, with around 10 % survival after two months of stress in presence of oxygen, without any significant effect of dehydration. These results highlight the potential of exploiting RAH fluctuations to develop control strategies targeting <em>C. perfringens</em> vegetative cells. However, the extreme resilience of spores confirms the need for specific and targeted decontamination methods to eliminate them effectively.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"135 ","pages":"Article 104968"},"PeriodicalIF":4.6,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145360681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial mist pretreatment for enhancing superheated steam efficacy in inactivating Enterococcus faecium NRRL B-2354 on dry food processing surface","authors":"Shruthy Seshadrinathan ,&nbsp;V.M. Balasubramaniam ,&nbsp;Abigail B. Snyder","doi":"10.1016/j.fm.2025.104956","DOIUrl":"10.1016/j.fm.2025.104956","url":null,"abstract":"<div><div>Superheated steam (SHS) is an emerging sanitation technology for dry food processing environments. This study investigates the efficacy of antimicrobial pretreatments in conjunction with SHS to inactivate microorganisms on stainless steel surfaces. Stainless steel coupons were inoculated with <em>Enterococcus faecium</em> (11.1 ± 0.4 log CFU/cm²), equilibrated to a water activity of 0.2 (a_w), and subjected to various antimicrobial pretreatments prior to SHS exposure. This includes soaking for 10 or 20 s in sterile water, 70 % ethanol, 0.05 % peracetic acid (mist applied for 5 s), or acidified oil emulsion (100 μL). SHS experiments were conducted using both bench-scale (100 % steam content) and pilot-scale equipment (5 % steam, 95 % hot air). The nozzle-to-surface distance was maintained at 3 cm. All experiments were performed in triplicate, and surviving microorganisms were enumerated using plate counts (detection limit: 0.2 log CFU/cm²). Antimicrobial pretreatments alone resulted in microbial reductions of &lt;2.0 ± 0.5 log CFU/cm². SHS treatment using the bench-scale system achieved &gt;6.4 ± 0.3 log CFU/cm² reduction regardless of pretreatment. Pilot-scale SHS treatment (with or without antimicrobials) achieved lower reductions (&lt;2.8 ± 0.2 log CFU/cm², <em>p</em> &lt; 0.05) possibly due to lower steam content. Increasing the treatment intensity (195 °C for 30 s) resulted moderate improvement in microbial inactivation (3.3 ± 0.6 log CFU/cm²). This study demonstrates that antimicrobial pretreatments can enhance the efficacy of SHS sanitation and highlights the importance of steam content in the treatment environment.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"135 ","pages":"Article 104956"},"PeriodicalIF":4.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145360682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the different saccharide metabolism patterns of unconventional interspecies brewery isolates","authors":"Yu Guan ,&nbsp;Qianyao Hou ,&nbsp;Chunfeng Liu ,&nbsp;Jinjing Wang ,&nbsp;Qi Li","doi":"10.1016/j.fm.2025.104954","DOIUrl":"10.1016/j.fm.2025.104954","url":null,"abstract":"<div><div>The fermentation performance of yeasts is pivotal in very high gravity (VHG) lager beer brewing, particularly in terms of their ability to consume maltose and maltotriose. In this study, we collected two unconventional interspecies brewery isolates used in lager beer brewing, which exhibit different saccharide metabolism patterns. A hybrid of <em>Saccharomyces cerevisiae</em> and <em>Saccharomyces kudriavzevii</em> had a higher affinity for both glucose and maltotriose, while another hybrid of <em>S. cerevisiae</em>, <em>Saccharomyces eubayanus,</em> and <em>Saccharomyces uvarum</em> could rapidly consume more maltose. Transcriptome and comparative genomics were analyzed based on their fermentation performance during VHG brewing. We found that mutated Sk-<em>IMAx</em> could be helpful for maltotriose usage, while Sk-<em>MIG2</em> could repress maltose consumption. Moreover, their different energy metabolism distribution also influenced the saccharide pattern greatly. The combination of heterosis from both <em>Saccharomyces</em> hybrids might shed light on innovation in VHG lager beer brewing and other diverse fermentation industries. Furthermore, a better understanding of cellular and genetic mechanisms could help us explore other possibilities in hybrids between <em>Saccharomyces</em> siblings.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"135 ","pages":"Article 104954"},"PeriodicalIF":4.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145323556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial-mycotoxin interactions in food: A review of ecotoxicological implications and omics approaches for understanding detoxification mechanisms","authors":"Charles Obinwanne Okoye ,&nbsp;Bonaventure Chidi Ezenwanne ,&nbsp;Olasoji Olufemi Olalowo ,&nbsp;Oma Judith Ajanwachukwu ,&nbsp;Kingsley Ikechukwu Chukwudozie","doi":"10.1016/j.fm.2025.104955","DOIUrl":"10.1016/j.fm.2025.104955","url":null,"abstract":"<div><div>Mycotoxins, toxic secondary metabolites produced by fungi such as <em>Aspergillus</em>, <em>Fusarium</em>, and <em>Penicillium</em>, frequently contaminate food supplies, posing risks to human health, animal welfare, and ecosystem stability. Mycotoxins like aflatoxins, ochratoxin A, fumonisins, trichothecenes, and zearalenone disrupt microbial communities, food chains, and environmental matrices, with synergistic interactions amplifying their toxicity. This review explores microbial-mycotoxin interactions in food systems, focusing on ecotoxicological implications and omics approaches for elucidating detoxification mechanisms. Microbial detoxification, through adsorption by cell wall components (e.g., β-glucans) or enzymatic biodegradation (e.g., lactonases, oxidoreductases), offers a sustainable alternative to physical and chemical methods. However, challenges include variable detoxification efficiency, mechanistic uncertainties, regulatory hurdles, and the detection of masked mycotoxins in complex food matrices. Omics technologies such as metagenomics, genomics, transcriptomics, and their integration provide comprehensive insights into microbial diversity, gene expression, enzyme activity, and metabolite transformations. In addition, omics integration enhances understanding of microbial-mycotoxin dynamics, supporting targeted biocontrol strategies. Future prospects include leveraging synthetic biology, CRISPR-based gene editing, and machine learning-assisted bioinformatics to optimize microbial strains and predict detoxification outcomes. By addressing these challenges, omics-driven approaches can mitigate mycotoxin contamination, ensure food safety, and reduce ecotoxicological impacts across global food systems.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"135 ","pages":"Article 104955"},"PeriodicalIF":4.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145323557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The antifungal potential and mechanistic action of durian shell water extract against citrus pathogen Fusarium oxysporum","authors":"Haiyan Lin ,&nbsp;Jingjing Shan ,&nbsp;Yineng Wang ,&nbsp;Yiliang Cai ,&nbsp;Shuo Shan ,&nbsp;Xianglong Chen ,&nbsp;Wei Hong ,&nbsp;Ruiyu Zhu","doi":"10.1016/j.fm.2025.104953","DOIUrl":"10.1016/j.fm.2025.104953","url":null,"abstract":"<div><div>Hongmeiren citrus<em>,</em> an economically important fruit, currently lacks targeted measures to control postharvest decay. While durian shells are rich in diverse antimicrobial compounds, their antifungal potential remains underexplored. In this study, <em>Fusarium oxysporum</em> was identified as a dominant pathogen causing rot in Hongmeiren citrus. We then evaluated the antifungal efficacy of durian shell water extract (DSWE) against <em>F. oxysporum in vitro</em> and further elucidated the underlying inhibitory mechanism through molecular docking analysis. Our results demonstrated that treatment with 4 mg/mL DSWE significantly reduced mycelial growth diameter by 77.2 % and completely suppressed sporulation by the 6th day post-inoculation, compared to the control (CK). Propidium iodide (PI) staining and scanning electron microscopy (SEM) revealed severe membrane damage and hyphal collapse in <em>F. oxysporum</em> treated with 4 mg/mL DSWE. Molecular docking predicted that three bioactive compounds in DSWE (fraxetin, taxifolin, and muscone) could bind to key proteins involved in the cell wall and membrane biosynthesis of <em>F. oxysporum</em>. Experimental validation confirmed that fraxetin, taxifolin, and muscone reduced the colony diameter of <em>F. oxysporum</em> by 62.8 %, 53.2 %, and 21.1 %, respectively, while inducing significant membrane injury. Notably, fraxetin and taxifolin exhibited superior antifungal activity compared to muscone. This study elucidates the mechanism by which DSWE inhibits <em>F. oxysporum</em> and highlights its potential as an eco-friendly strategy for controlling postharvest pathogens in citrus.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"135 ","pages":"Article 104953"},"PeriodicalIF":4.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145323482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conditional synergy: Impact of nutrient abundance on multispecies biofilm formation and sanitizer tolerance","authors":"Krisha Pant,&nbsp;Jon Palmer,&nbsp;Steve Flint","doi":"10.1016/j.fm.2025.104952","DOIUrl":"10.1016/j.fm.2025.104952","url":null,"abstract":"<div><div>Bacteria exist in varying nutrient conditions and complex microbial consortia. <em>Pseudomonas fluorescens</em>, <em>Staphylococcus aureus</em>, and <em>Listeria monocytogenes</em> are commonly occurring biofilm-formers, share a similar nutritional niche, and have been isolated from common surfaces in multispecies environments. Biofilm properties, including biomass (O.D₅₉₀ nm), cell concentration (log CFU/cm²), exopolysaccharide content (μg/cm²), structure, and sanitizer tolerance (sodium hypochlorite), were observed under varying nutrient (full-strength TSB and 10 % TSB) conditions on polystyrene surfaces for single and multispecies biofilm. The synergistic interactions between the bacteria in multispecies biofilm were found to be nutrient-dependent, with significantly higher (p &lt; 0.05) biofilm formation, exopolysaccharide content, and sanitizer tolerance in high nutrient conditions (TSB) compared with low nutrient conditions (10 % TSB). The cell concentrations in the biofilm (single and multispecies) were found to be comparable between TSB and 10 % TSB. All three bacteria involved showed increased tolerance against sanitizers in the multispecies arrangement compared to their single-species counterparts, with significantly higher survival for <em>L. monocytogenes</em> (5.3 log CFU/cm²) in a multispecies biofilm compared to its single-species counterpart (2.3 log CFU/cm²). A positive correlation was observed between exopolysaccharide concentration and sanitizer tolerance. This study highlights the importance of taking multiple bacteria and their growth environment into account when understanding sanitizer response, as it varies in multispecies biofilm setups and according to nutrient availability.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"134 ","pages":"Article 104952"},"PeriodicalIF":4.6,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic factors contributing to the resilience of Salmonella enterica on ready-to-eat muskmelon","authors":"Irene Esteban-Cuesta ,&nbsp;Laura Führer ,&nbsp;Steffen Porwollik ,&nbsp;Weiping Chu ,&nbsp;Steven R. Fiddaman ,&nbsp;Irmak Sah ,&nbsp;Michael McClelland ,&nbsp;Claudia Guldimann","doi":"10.1016/j.fm.2025.104947","DOIUrl":"10.1016/j.fm.2025.104947","url":null,"abstract":"<div><div><em>Salmonella</em> outbreaks have repeatedly been associated with muskmelons. To identify genes under selection in <em>S. enterica</em> growing in this food matrix, barcoded transposon mutant libraries in three <em>S. enterica</em> serovars <em>-</em> Typhimurium, Enteritidis, and Newport - were screened for survival and growth on muskmelon. Applying stringent thresholds, a total of 26 genes in Typhimurium, 34 in Enteritidis, and 50 in Newport were found to significantly influence fitness during muskmelon interaction, with many of these being temperature dependent. Genes whose disruption affected fitness across all three serovars were enriched for functions related to RNA degradation and ribosome biogenesis. Targeted competition assays confirmed the contribution of selected genes, revealing nutrient-dependent phenotypes for most mutants. Remarkably, the polyribonucleotide nucleotidyltransferase gene, <em>pnp,</em> and the D-3-phosphoglycerate dehydrogenase gene, <em>serA</em>, conferred a selective advantage when growing in muskmelon but not under nutrition-rich control conditions. In contrast, the nitrogen regulation response regulator GlnG provided a muskmelon-specific fitness disadvantage. This study provides novel insights into genome-wide adaptation mechanisms of multiple <em>Salmonella</em> serovars to growth on muskmelons, revealing both shared and serovar-specific determinants while illustrating the dynamic genetic responses of <em>S. enterica</em> throughout the interaction period.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"134 ","pages":"Article 104947"},"PeriodicalIF":4.6,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145332596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of acetic acid in Cabernet Sauvignon wine fermented by atmospheric and room temperature plasma-mutagenized Saccharomyces cerevisiae","authors":"Nan Wu ,&nbsp;Xiaoqing Zhang ,&nbsp;Erhu Li","doi":"10.1016/j.fm.2025.104951","DOIUrl":"10.1016/j.fm.2025.104951","url":null,"abstract":"<div><div>Acetic acid, a major byproduct of <em>Saccharomyces cerevisiae</em> alcoholic fermentation, hinder fermentation efficiency and wine quality. This study improved wine yeast strains through ARTP mutagenesis, resulting in mutant DC-3 with enhanced fermentation performance and reduced acetic acid production. Analysis of DC-3 showed improved oenological properties, including a 25.84 % decrease in acetic acid production compared to wild-type strain RV002 in synthetic grape juice. The study identified a regulatory mechanism in DC-3 that reduces acetate production by suppressing pyruvate decarboxylase, enhancing alcohol dehydrogenase, and inhibiting acetaldehyde dehydrogenase activities, while redirecting metabolism towards glycerol biosynthesis. Transcriptomic analysis confirmed this metabolic shift. DC-3 consistently produced lower acetic acid levels and exhibited a unique volatile profile with higher alcohols and lower esters, specifically reducing acetate esters compared to RV002 in Cabernet Sauvignon wine. This study introduces a novel approach to breeding low-acetate-producing <em>S. cerevisiae</em> strains in Cabernet Sauvignon wine fermentation using ARTP mutagenesis. It lays the groundwork for enhancing wine fermentation processes.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"134 ","pages":"Article 104951"},"PeriodicalIF":4.6,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145266423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}