Food microbiology最新文献_第9页

Persistence and localization of Salmonella enterica subsp. enterica Javiana and Listeria monocytogenes during recirculating hydroponic cultivation of leaf lettuce 肠沙门氏菌亚种的存在与定位。叶莴苣循环水耕栽培中的贾维氏肠菌和单核增生李斯特菌

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-10-06 DOI: 10.1016/j.fm.2025.104950

Gayatri Rajashekhar Dhulappanavar , Zeynal Topalcengiz , Ryan W. Dickson , Kristen E. Gibson

The persistence and localization of Salmonella Javiana and Listeria monocytogenes in recirculating deep water culture (DWC) hydroponic systems during lettuce production were investigated. Recirculating DWC systems containing modified Hoagland's nutrient solution (NS) were utilized. The NS reservoirs of DWC systems containing 25-day old butterhead lettuce (cv. Rex) seedlings were inoculated separately with 6 log CFU/mL of S. Javiana or rifampicin resistant L. monocytogenes. Pathogens were enumerated in NS, rockwool + roots, roots, and leaves post inoculation from day 0 (lettuce seedling) to day 21 (mature lettuce head) via culture on selective media. Four experimental trials were completed for each pathogen with 2 treated and 2 control systems in each trial. Temperature, pH, and electrical conductivity data were collected and considered covariates during Analysis of Covariance (ANCOVA). The data were analyzed using a mixed model to compare mean log bacteria concentration by pathogen type based on sample type and sampling day followed by calculation of least-squares means where values were compared with Tukey-Kramer honest significant difference test at P = 0.05. A two-way interaction between sampling day and sample type (P < 0.0001) had a significant effect on S. Javiana and L. monocytogenes concentrations. The ANCOVA indicated that the interaction effect of NS pH and sample type had a significant effect on S. Javiana (P = 0.0054) and L. monocytogenes (P < 0.0001) concentrations. Salmonella Javiana and L. monocytogenes persisted in roots, rockwool + roots, and NS throughout the 21-day sampling period. No pathogens were detected in the edible portion of the lettuce. This study indicates that human pathogens can survive until harvest in DWC hydroponics systems used for lettuce production.

研究了深水循环培养（DWC）水培系统中贾维纳沙门氏菌和单核增生李斯特菌在生菜生产过程中的持久性和定位。采用含有改良Hoagland营养液（NS）的循环DWC系统。含25日龄油头莴苣的DWC系统的NS储层(cv。分别接种6 log CFU/mL的Javiana或利福平耐药单增李斯特菌。从第0天（生菜幼苗）到第21天（生菜成熟头），通过选择培养基培养，在接种后的NS、岩棉+根、根和叶中枚举病原体。每种病原体完成4个试验，每个试验有2个处理系统和2个控制系统。在协方差分析（ANCOVA）中，收集温度、pH值和电导率数据并将其视为协变量。采用混合模型比较不同样品类型和采样天数的病原菌类型平均对数细菌浓度，计算最小二乘均值，其中采用Tukey-Kramer诚实显著性差异检验，P = 0.05。取样天数和取样类型之间存在双向交互作用（P < 0.0001），对Javiana和L.单核增生菌浓度有显著影响。ANCOVA结果表明，NS pH和样品类型的交互作用对S. Javiana （P = 0.0054）和L.单核细胞增生（P < 0.0001）浓度有显著影响。在21天的采样期内，沙门菌和单核增生L.菌在根、岩棉+根和土壤中持续存在。生菜的可食用部分未检测到病原体。这项研究表明，人类病原体可以在用于生菜生产的DWC水培系统中存活到收获。

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引用次数: 0

A genomic view on lactic metabolism 乳酸代谢的基因组观点

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-10-06 DOI: 10.1016/j.fm.2025.104945

Nanzhen Qiao, Michael G. Gänzle

Lactic acid bacteria relate in many ways to the well-being and the economic activity of humans. Lactic acid bacteria are recognised as human pathogens but also are beneficial members of commensal microbial communities in humans and animals, and are used as probiotics. Lactic acid bacteria impact food quality and safety both as beneficial fermentation microbes and as spoilage organisms. Owing to their multi-faceted relationship to humans, the carbohydrate metabolism of lactic acid bacteria has been subject to research for more than a century. The aim of this review is to link the wealth of knowledge on lactic metabolism to the currently available genomic information on lactic acid bacteria.

Homofermentative and heterofermentative lactic acid bacteria take a substantially different approach to energy generation, have different substrate preferences and thus co-exist in multiple ecological niches. Many lactic acid bacteria maintain electron transfer chains to use oxygen, nitrate or iron as terminal electron acceptors under suitable conditions. The metabolism of organic acids and diols contributes to acid resistance and support stationary-phase survival. The ecology of lactic acid bacteria also shapes metabolic preferences and several metabolic traits differentiate insect associated, vertebrate-host adapted, free living and domesticated lactic acid bacteria. Knowledge of metabolic preferences that relate to phylogeny or the adaptation to different ecological niches facilitates selection of starter cultures for conventional and novel food fermentations.

乳酸菌在许多方面与人类的福祉和经济活动有关。乳酸菌被认为是人类病原体，但也是人类和动物共生微生物群落的有益成员，并被用作益生菌。乳酸菌作为有益的发酵微生物和腐败微生物影响食品质量和安全。由于乳酸菌与人类有着多方面的关系，一个多世纪以来，人们一直在研究乳酸菌的碳水化合物代谢。这篇综述的目的是将丰富的乳酸代谢知识与目前可用的乳酸菌基因组信息联系起来。同发酵型和异发酵型乳酸菌产生能量的方式完全不同，对底物的偏好也不同，因此在多个生态位中共存。许多乳酸菌在适当的条件下维持电子传递链，以氧、硝酸盐或铁作为终端电子受体。有机酸和二醇的代谢有助于抗酸和支持稳定期生存。乳酸菌的生态也决定了它们的代谢偏好，一些代谢特征区分了昆虫相关的、脊椎动物宿主适应的、自由生活的和驯化的乳酸菌。与系统发育或适应不同生态位有关的代谢偏好的知识有助于选择传统和新型食品发酵的发酵剂。

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引用次数: 0

Redefining LuxI as a metabolic gatekeeper in bacterial spoilage of refrigerated turbot by Hafnia alvei H4 重新定义LuxI作为冷藏大比目鱼被Hafnia alvei H4细菌破坏的代谢守门人

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-10-05 DOI: 10.1016/j.fm.2025.104949

Xue Li , Hongman Hou

Refrigerated seafood spoilage, a major threat to food safety and quality, is primarily driven by specific spoilage organisms whose molecular mechanisms remain insufficiently elucidated. Here, we demonstrate that Hafnia alvei H4, a dominant spoiler in turbot, accelerates spoilage by deploying a LuxI-mediated metabolic override to disrupt putrescine homeostasis. Genetic knockout studies showed that luxI deletion (ΔluxI), but not luxR, most effectively suppressed spoilage, reducing putrescine and total volatile basic nitrogen (TVB-N) by 58 % and 52 %, respectively, compared to the wild type. Regression analysis established putrescine and cadaverine as an indicator of spoilage in aquatic products, strongly correlated with TVB-N (R² > 0.966, p < 0.05). Subsequent induction assays with ornithine/putrescine uncovered a >110 mg/L feedback inhibition threshold, while genomic analysis mapped PotE as the primary efflux transporter. Real-time transport monitoring revealed that LuxI sustains extracellular putrescine at levels exceeding 500 mg/L—far above the inhibition threshold—effectively activating proteolytic pathways. This work position LuxI as a metabolic gatekeeper and offer a mechanistic basis for novel quorum sensing-targeted strategies to mitigate seafood spoilage.

冷藏海鲜的腐败是对食品安全和质量的主要威胁，主要是由特定的腐败微生物引起的，其分子机制尚不清楚。在这里，我们证明了Hafnia alvei H4，一种主要的大菱破坏者，通过部署luxi介导的代谢覆盖来破坏腐胺稳态，从而加速腐败。基因敲除研究表明，与野生型相比，luxI缺失（ΔluxI）而不是luxR最有效地抑制了腐败，腐胺和总挥发性碱性氮（TVB-N）分别降低了58%和52%。回归分析表明腐胺和尸胺是水产品腐败的指标，与TVB-N呈强相关（R2 > 0.966, p < 0.05）。随后的鸟氨酸/腐胺诱导试验发现了110 mg/L的反馈抑制阈值，而基因组分析则将PotE定位为主要的外排转运蛋白。实时运输监测显示，LuxI维持超过500 mg/ l的细胞外腐胺水平-远高于抑制阈值-有效激活蛋白水解途径。这项工作将LuxI定位为代谢守门人，并为新的群体感应靶向策略提供机制基础，以减轻海鲜腐败。

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引用次数: 0

Verification of the esters-producing properties of Thermoascus aurantiacus QH-1 derived from low-temperature Daqu by multiomics 低温大曲衍生金斑热曲霉QH-1产酯特性的多组学验证

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-10-04 DOI: 10.1016/j.fm.2025.104946

Mengjiao Yuan , Tong Lu , Hongxia Zhang , Jiali Wang , Meilin Cui , Wei Zhao , Yanfang Wu , Xiaozhuang Ren , Pengbo Yao , Xiuhong Zhang

Thermoascus aurantiacus is a dominant microorganism in diverse high-temperature Daqu samples, and even in low-temperature Daqu. However, its role in producing ester-based aromatic compounds remains incompletely understood. This study employed multi-omics approaches to characterize the esters-producing capacity of T. aurantiacus QH-1 derived from low-temperature Daqu used for light-flavor Baijiu brewing. Genome sequencing uncovered 58 genes encoding enzymes involved in esters metabolism, while only 23 ester-metabolizing enzymes were identified with proteomics analysis. When the fungal cultures of T. aurantiacus QH-1 at 40 °C, 45 °C, and 50 °C were analyzed by metabolomics approach, respectively, a total of 56 kinds of volatile esters flavor compounds were detected, including 24 ethyl esters which are key contributors to Baijiu flavor. Ethyl acetate, the most important aroma ingredient in light-flavor Baijiu, was the most abundant ester across all temperature conditions. Enzymatic properties of the crude esterase from T. aurantiacus QH-1 revealed the optimal condition was at 50 °C and pH 5, with acetic acid significantly enhanced enzymatic activity. These results indicated that T. aurantiacus QH-1 could produce lots of esters flavor compounds, established it as a primary functional microorganism responsible for imparting aromatic compounds for Daqu.

金热曲霉（Thermoascus aurantiacus）是各种高温大曲样品中的优势微生物，甚至在低温大曲中也是如此。然而，它在生成酯基芳香族化合物中的作用仍不完全清楚。本研究采用多组学方法，对用于酿造淡味白酒的低温大曲中提取的T. aurantiacus QH-1产酯能力进行了研究。基因组测序发现了58个编码酯代谢酶的基因，而蛋白质组学分析仅鉴定出23个酯代谢酶。采用代谢组学方法对40℃、45℃和50℃条件下的金酸霉QH-1真菌培养物进行分析，共检测到56种挥发性酯类风味化合物，其中24种乙酯类是白酒风味的关键成分。在所有温度条件下，淡味白酒中最重要的香气成分乙酸乙酯含量最高。结果表明，在50℃、pH为5的条件下，粗酯酶的酶活性得到显著提高。这些结果表明，T. aurantiacus QH-1可以产生大量的酯类风味化合物，确定了它是大曲传授芳香化合物的主要功能微生物。

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引用次数: 0

Volatolomics reveals the influence of O2/CO2 on the growth of Pseudomonas fragi and its volatile metabolites on meat substrates 挥发组学揭示了O2/CO2对肉基质上假单胞菌及其挥发性代谢物生长的影响

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-10-01 DOI: 10.1016/j.fm.2025.104941

Linyun Chen , María Isabel Almeida , Lotta Kuuliala , Christophe Walgraeve , Kristof Demeestere , Frank Devlieghere

Pork is highly susceptible to microbial spoilage. The growth and metabolism of spoilage bacteria cause the formation of malodorous volatile organic compounds (VOCs), leading to sensory decline. Even though the atmosphere is a well-known extrinsic factor affecting microbial growth, the fundamental impact of bulk gas ratios on VOC production still needs to be further elaborated. The present research focuses on Pseudomonas fragi, one of the most dominant spoilage microbes in packaged meat, by studying its behavior as influenced by atmospheres. Solid pork simulation media inoculated with a pure meat-derived P. fragi strain were stored under four conditions with different bulk gas ratios (v/v%

O_{2} / {CO}_{2} / N_{2}

: air, 0/0/100, 20/20/60, 20/40/40). For each atmosphere, characteristic microbial VOCs were identified by combining online and offline chemical analytical tools, namely selected-ion flow-tube mass spectrometry (SIFT-MS) and thermal-desorption gas chromatography mass spectrometry (TD-GC-MS). In total, fourteen compounds were linked to the investigated P. fragi strain, including methyl acetate, ethyl acetate, methyl mercaptan, and dimethyl sulfide. Despite slower microbial growth under 0/0/100 and 20/20/60 when compared to air, anaerobic metabolites including sulfur-containing compounds and ethyl acetate were produced in high quantities at a lower microbial level. On the contrary, 40%

{CO}_{2}

largely limited bacterial counts and VOCs. Overall, comprehensive volatolome analysis provides informative insights into the P. fragi metabolism and its relation with the atmosphere, thus improving the understanding of meat spoilage mechanisms.

猪肉很容易受到微生物的腐蚀。腐败菌的生长和代谢导致恶臭挥发性有机物（VOCs）的形成，导致感官衰退。尽管大气是众所周知的影响微生物生长的外在因素，但体积气体比对VOC产生的根本影响仍需要进一步阐述。本文对包装肉类中最主要的腐败微生物之一fragi假单胞菌进行了研究，研究了其在环境影响下的行为。用一株纯肉源性fragi菌株接种的固体猪肉模拟培养基，在不同体积气体比（v/v% O2/CO2/N2：空气、0/0/100、20/20/60、20/40/40）的条件下保存。通过结合在线和离线化学分析工具，即选择离子流管质谱法（SIFT-MS）和热解吸气相色谱质谱法（TD-GC-MS），对每种大气中的特征微生物VOCs进行了鉴定。总共有14种化合物与所研究的fragi菌株有关，包括乙酸甲酯、乙酸乙酯、甲基硫醇和二甲基硫化物。尽管与空气相比，0/0/100和20/20/60条件下的微生物生长速度较慢，但在较低的微生物水平下，厌氧代谢物（包括含硫化合物和乙酸乙酯）的产量很高。相反，40%的二氧化碳在很大程度上限制了细菌数量和挥发性有机化合物。总的来说，全面的挥发组分析提供了对fragi P.代谢及其与大气关系的信息见解，从而提高了对肉类腐败机制的理解。

{"title":"Volatolomics reveals the influence of O2/CO2 on the growth of Pseudomonas fragi and its volatile metabolites on meat substrates","authors":"Linyun Chen , María Isabel Almeida , Lotta Kuuliala , Christophe Walgraeve , Kristof Demeestere , Frank Devlieghere","doi":"10.1016/j.fm.2025.104941","DOIUrl":"10.1016/j.fm.2025.104941","url":null,"abstract":"<div><div>Pork is highly susceptible to microbial spoilage. The growth and metabolism of spoilage bacteria cause the formation of malodorous volatile organic compounds (VOCs), leading to sensory decline. Even though the atmosphere is a well-known extrinsic factor affecting microbial growth, the fundamental impact of bulk gas ratios on VOC production still needs to be further elaborated. The present research focuses on <em>Pseudomonas fragi</em>, one of the most dominant spoilage microbes in packaged meat, by studying its behavior as influenced by atmospheres. Solid pork simulation media inoculated with a pure meat-derived <em>P. fragi</em> strain were stored under four conditions with different bulk gas ratios (v/v% <span><math><mrow><msub><mrow><mi>O</mi></mrow><mrow><mn>2</mn></mrow></msub><mo>/</mo><msub><mrow><mi>CO</mi></mrow><mrow><mn>2</mn></mrow></msub><mo>/</mo><msub><mrow><mi>N</mi></mrow><mrow><mn>2</mn></mrow></msub></mrow></math></span>: air, 0/0/100, 20/20/60, 20/40/40). For each atmosphere, characteristic microbial VOCs were identified by combining online and offline chemical analytical tools, namely selected-ion flow-tube mass spectrometry (SIFT-MS) and thermal-desorption gas chromatography mass spectrometry (TD-GC-MS). In total, fourteen compounds were linked to the investigated <em>P. fragi</em> strain, including methyl acetate, ethyl acetate, methyl mercaptan, and dimethyl sulfide. Despite slower microbial growth under 0/0/100 and 20/20/60 when compared to air, anaerobic metabolites including sulfur-containing compounds and ethyl acetate were produced in high quantities at a lower microbial level. On the contrary, 40% <span><math><msub><mrow><mi>CO</mi></mrow><mrow><mn>2</mn></mrow></msub></math></span> largely limited bacterial counts and VOCs. Overall, comprehensive volatolome analysis provides informative insights into the <em>P. fragi</em> metabolism and its relation with the atmosphere, thus improving the understanding of meat spoilage mechanisms.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"134 ","pages":"Article 104941"},"PeriodicalIF":4.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TrveA affects the pathogenicity to apples by regulating the sporulation, reactive oxygen species and trichothecenes metabolic pathways in Trichothecium roseum TrveA通过调控玫瑰曲霉的产孢、活性氧和曲霉烯代谢途径影响苹果的致病性

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-27 DOI: 10.1016/j.fm.2025.104943

Qili Liu , Xiaobin Xu , Yangyang Yang , Xiao Li , Pengfei Sun , Pengdong Xie , Yuanyuan Zong , Huali Xue , Yang Bi

Trichothecium roseum, the causative agent of core rot of apple fruit, is one of the most important postharvest pathogenic fungi. In addition to quality degradation and economic influence, apple fruits infected by T. roseum can metabolize trichothecene that pose a potential threat to human health. VeA is a pivotal regulator of growth, development, pathogenicity and secondary metabolism for fungi. However, the function and regulatory mechanism of TrveA in T. roseum infecting apple are still unclear. In this study, the TrveA mutant was constructed by Split-marker PCR technology, and the effects of TrveA on the pathogenicity and toxin accumulation of T. roseum infecting apple and tomato were analyzed. The regulation of spore formation, oxidative stress tolerance, reactive oxygen species (ROS) metabolism and T-2 toxin biosynthesis was explored to elucidate the potential mechanism of TrveA regulating the pathogenicity of T. roseum. The results suggested that the deletion of TrveA inhibited the growth and development of the mutant, down-regulated the expression of key regulatory genes fluG, flbA, abaA and wetA in the sporulation pathway, and interfered with trehalose biosynthesis, resulting in a decrease in sporulation. TrveA deletion reduced the level of ROS accumulation and weakened the capacity of ROS scavenging, which cut down its tolerance to oxidative stress. At the same time, the expression of key genes tri6, tri5 and tri4 involved in the T-2 toxin biosynthesis pathway was down-regulated to inhibit the biosynthesis of T-2 toxin, which eventually led to the decrease of pathogenicity to apple and tomato. This study reveals the important role of TrveA in T. roseum infecting apple fruits, which is conductive to better elucidate the regulatory mechanism of TrveA. These results will provide potential precise targets for curbing apple postharvest diseases caused by T. roseum.

玫瑰曲霉是苹果果心腐病的病原菌，是重要的采后致病真菌之一。除了品质退化和经济影响外，被玫瑰曲菌感染的苹果果实还会代谢对人体健康构成潜在威胁的毛霉烯。VeA是真菌生长发育、致病性和次生代谢的关键调节因子。然而，TrveA在玫瑰病侵染苹果中的作用和调控机制尚不清楚。本研究利用分裂标记PCR技术构建了TrveA突变体，分析了TrveA对苹果和番茄玫瑰红霉致病性和毒素积累的影响。通过对孢子形成、氧化应激耐受性、活性氧（ROS）代谢和T-2毒素生物合成的调控，探讨TrveA调控玫瑰红霉致病性的潜在机制。结果表明，TrveA的缺失抑制了突变体的生长发育，下调了产孢途径中关键调控基因fluG、flbA、abaA和wetA的表达，干扰了海藻糖的生物合成，导致产孢量减少。TrveA缺失降低了ROS积累水平，削弱了ROS清除能力，从而降低了其对氧化应激的耐受性。同时，下调T-2毒素生物合成途径关键基因tri6、tri5和tri4的表达，抑制T-2毒素的生物合成，最终导致对苹果和番茄的致病性降低。本研究揭示了TrveA在玫瑰曲霉侵染苹果果实中的重要作用，有助于更好地阐明TrveA的调控机制。这些结果将为防治苹果采后病害提供潜在的精确靶点。

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引用次数: 0

Probing the repair mechanism of ohmic heating-induced sublethal injury in Staphylococcus aureus: Multi-omics analysis uncovers the remodeling patterns of proteins and lipids and applications in simulated food systems 探索欧姆加热诱导的金黄色葡萄球菌亚致死损伤的修复机制：多组学分析揭示了蛋白质和脂质的重塑模式及其在模拟食物系统中的应用

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-26 DOI: 10.1016/j.fm.2025.104944

Han Wang , Bo Zou , Yana Liu , Yingying Sun , Yan Zhao , Yi Liu , Xingmin Li , Ruitong Dai

Ohmic heating (OH), a novel food processing technology, has been shown to effectively inactivate Staphylococcus aureus (S. aureus). However, the molecular repair mechanisms of OH-induced sublethal injury in S. aureus remain unclear. In this study, four experimental groups were established: intact cells (UT), OH-injured cells without repair (R0), and injured cells repaired in NB medium for either 15 min (R1, early repair stage) or 2 h (R2, mid-repair stage). Atomic force microscopy revealed that cell morphology was disrupted by OH treatment (R0) but progressively restored during repair (R0–R2). Integrated proteomic and lipidomic analyses demonstrated that OH treatment suppressed proteins and lipids involved in membrane integrity, energy metabolism, and amino acid metabolism, reflecting an adaptive stress response. During the early repair stage, upregulation of these molecules indicated activation of recovery pathways, whereas their downregulation in the mid-repair stage suggested stabilization of cellular functions. Intracellular ion concentrations and enzyme activities were consistent with proteomic trends, while lipidomics highlighted dynamic remodeling of key membrane lipids such as phosphatidylglycerol and cardiolipin. In conclusion, this study provides new insights into the molecular mechanisms underlying OH-induced injury and repair in S. aureus, advancing the understanding of bacterial stress adaptation and offering guidance for optimizing OH technology in food safety applications.

欧姆加热（OH）是一种新型的食品加工技术，已被证明可以有效地灭活金黄色葡萄球菌（S. aureus）。然而，oh诱导的金黄色葡萄球菌亚致死损伤的分子修复机制尚不清楚。本研究建立了四个实验组：完整细胞（UT）、oh损伤未修复细胞（R0）和损伤细胞在NB培养基中修复15 min （R1，修复早期）或2 h （R2，修复中期）。原子力显微镜显示，OH处理（R0）破坏了细胞形态，但在修复过程中逐渐恢复（R0 - r2）。综合蛋白质组学和脂质组学分析表明，OH处理抑制了参与膜完整性、能量代谢和氨基酸代谢的蛋白质和脂质，反映了适应性应激反应。在早期修复阶段，这些分子的上调表明恢复途径的激活，而在修复中期，它们的下调表明细胞功能的稳定。细胞内离子浓度和酶活性与蛋白质组学趋势一致，而脂质组学强调关键膜脂如磷脂酰甘油和心磷脂的动态重塑。总之，本研究对OH诱导金黄色葡萄球菌损伤和修复的分子机制提供了新的见解，促进了对细菌应激适应的认识，并为OH技术在食品安全中的优化应用提供了指导。

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引用次数: 0

Corrigendum to “Study on the biocontrol efficacy of Meyerozyma caribbica against blue mold of pears and the mechanisms involved” [Food Microbiol. Volume 134, March 2026, 104917] 《梨蓝霉防治效果及机制研究》[食品微生物学]。第134卷，2026年3月，104917]

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-26 DOI: 10.1016/j.fm.2025.104933

Mingjian Zhang , Yujie He , Luyi Liang , Solairaj Dhanasekaran , Yize Hu , Xiaoyun Zhang , Jun Li , Lina Zhao , Hongyin Zhang

引用次数: 0

Integrated multi-omics and physiological analyses reveal mechanism of enhanced gellan gum biosynthesis in an ARTP-induced Sphingomonas paucimobilis mutant 综合多组学和生理分析揭示了artp诱导的少动鞘单胞菌突变体中结冷胶生物合成增强的机制

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-26 DOI: 10.1016/j.fm.2025.104942

Ling Sun , Shiyi Chen , Yazhen Wang , Lei Sun , Zejian Wang , Yong Zhou , Ali Mohsin , Suyang Li , Luning Zhao , Afira Nayab , Wenjing Sun

Gellan gum demonstrates superior gelation properties and has been widely applied. However, the biosynthesis mechanism of gellan gum remains poorly understood, hampering efforts to increase its production. Therefore, this study comprehensively analyzed the phenotypes of a high-yield gellan gum mutant strain ATCC31461-M519 induced by ARTP treatment, its gellan gum characteristics and potential molecular mechanisms of gellan gum biosynthesis. Compared to the original strain ATCC31461, the mutant strain ATCC31461-M519 demonstrated a 59.0 % increase in gellan gum yield (5-L fermenter) along with enhanced sugar-to-gel conversion efficiency, enlarged cell size (1.72-fold length), and elevated cell permeability. Notably, its functional group composition, monosaccharide profile (glucose: rhamnose: glucuronic acid = 2.45 : 1: 0.81), and molecular weight (1.137 × 10⁶ g/mol) remained unaltered. Whole-genome re-sequencing identified 245 SNPs and 35 InDels in the genome of the mutant strain, while RNA-seq revealed 390 differentially expressed genes (DEGs: 107 up-regulated, 283 down-regulated). Integrative multi-omics analysis revealed that the two-component system (TCS) pathway was significantly enriched in the mutant strain with 4 InDel mutated genes and 45 DEGs. Moreover, qRT-PCR analysis confirmed the expression of gelS, gelC, gelE, gelG (gellan gum synthase) in the mutant strain significantly increased by approximately 8.4, 8.6, 6.2, 5.5 fold change, respectively, and its lyase (gelR) expression decreased by 84.7 %. Therefore, the mutated genes and DEGs in the TCS pathway, up-regulated expression of synthetase, and enhanced cell membrance permeability and surface area jointly improved the production of gellan gum, which provides molecular basis to increase gellan gum production by using metabolic engineering.

结冷胶具有优良的胶凝性能，得到了广泛的应用。然而，结冷胶的生物合成机制仍然知之甚少，阻碍了努力增加其产量。因此，本研究综合分析了ARTP诱导的结冷胶高产突变株ATCC31461-M519的表型、结冷胶特性以及结冷胶生物合成的潜在分子机制。与原始菌株ATCC31461相比，突变菌株ATCC31461- m519的结冷胶产量（5-L发酵量）提高了59.0%，糖-凝胶转化效率提高，细胞大小增大（长度为1.72倍），细胞通透性提高。值得注意的是，其官能团组成、单糖谱（葡萄糖：鼠李糖：葡萄糖醛酸= 2.45:1:0 .81）和分子量（1.137 × 106 g/mol）保持不变。全基因组重测序鉴定出245个snp和35个InDels， RNA-seq鉴定出390个差异表达基因（DEGs: 107个上调，283个下调）。综合多组学分析显示，具有4个InDel突变基因和45个deg的突变菌株的双组分系统（TCS）通路显著富集。qRT-PCR分析证实，突变菌株凝胶、gelC、gelE、gelG（结冷胶合成酶）的表达量分别增加了约8.4倍、8.6倍、6.2倍、5.5倍，裂解酶（gelR）的表达量下降了84.7%。因此，TCS通路中突变的基因和DEGs、合成酶表达上调、细胞膜通透性和表面积增强共同促进了结冷胶的生成，为利用代谢工程增加结冷胶的生成提供了分子基础。

{"title":"Integrated multi-omics and physiological analyses reveal mechanism of enhanced gellan gum biosynthesis in an ARTP-induced Sphingomonas paucimobilis mutant","authors":"Ling Sun , Shiyi Chen , Yazhen Wang , Lei Sun , Zejian Wang , Yong Zhou , Ali Mohsin , Suyang Li , Luning Zhao , Afira Nayab , Wenjing Sun","doi":"10.1016/j.fm.2025.104942","DOIUrl":"10.1016/j.fm.2025.104942","url":null,"abstract":"<div><div>Gellan gum demonstrates superior gelation properties and has been widely applied. However, the biosynthesis mechanism of gellan gum remains poorly understood, hampering efforts to increase its production. Therefore, this study comprehensively analyzed the phenotypes of a high-yield gellan gum mutant strain ATCC31461-M519 induced by ARTP treatment, its gellan gum characteristics and potential molecular mechanisms of gellan gum biosynthesis. Compared to the original strain ATCC31461, the mutant strain ATCC31461-M519 demonstrated a 59.0 % increase in gellan gum yield (5-L fermenter) along with enhanced sugar-to-gel conversion efficiency, enlarged cell size (<em>1.72-fold length</em>), and elevated cell permeability. Notably, its functional group composition, monosaccharide profile (glucose: rhamnose: glucuronic acid = 2.45 : 1: 0.81), and molecular weight (1.137 × 10<sup>6</sup> g/mol) remained unaltered. Whole-genome re-sequencing identified 245 SNPs and 35 InDels in the genome of the mutant strain, while RNA-seq revealed 390 differentially expressed genes (DEGs: 107 up-regulated, 283 down-regulated). Integrative multi-omics analysis revealed that the two-component system (TCS) pathway was significantly enriched in the mutant strain with 4 InDel mutated genes and 45 DEGs. Moreover, qRT-PCR analysis confirmed the expression of <em>gelS</em>, <em>gelC</em>, <em>gelE</em>, <em>gelG</em> (gellan gum synthase) in the mutant strain significantly increased by approximately 8.4, 8.6, 6.2, 5.5 fold change, respectively, and its lyase (<em>gelR</em>) expression decreased by 84.7 %. Therefore, the mutated genes and DEGs in the TCS pathway, up-regulated expression of synthetase, and enhanced cell membrance permeability and surface area jointly improved the production of gellan gum, which provides molecular basis to increase gellan gum production by using metabolic engineering.</div></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":"134 ","pages":"Article 104942"},"PeriodicalIF":4.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and characteristics of Campylobacter spp. in turkeys at slaughter 屠宰火鸡弯曲杆菌的分布和特征

IF 4.6 1区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food microbiology

Pub Date : 2025-09-25 DOI: 10.1016/j.fm.2025.104938

Laura Blomvall , Rauni Kivistö , Anniina Jaakkonen , Satu Olkkola , Maria Fredriksson-Ahomaa

Campylobacter is the leading cause of bacterial foodborne gastrointestinal infection in humans, and poultry is among the most important reservoirs. Turkeys have been shown to frequently carry Campylobacter, and the isolates recovered from turkeys show high antimicrobial resistance. We studied the prevalence of Campylobacter in Finnish slaughter turkeys during 2013–2023 from the monitoring data of a Finnish turkey slaughterhouse, covering 1856 turkey flocks originating from 41 farms over the monitoring period. Additionally, we used whole-genome sequencing to identify bacterial species, resistance profiles, sequence types, and core genome allelic profiles for 103 Campylobacter isolates from turkey faecal samples collected at the slaughterhouse between 2013 and 2021. In total, 9.5 % of the flocks and 82.9 % of the farms were Campylobacter positive. The prevalence varied significantly between years, months, and farms. Campylobacter jejuni (87.6 %) was the most common species followed by Campylobacter coli (4.0 %) and Campylobacter lari (1.1 %). We obtained 34 sequence types, with ST45 being the most common (20.2 % of the isolates). Genetically closely related isolates originated mostly from the same farm with a tight temporal connection. Five antimicrobial genotypic resistance profiles were identified. Most of the isolates carried only the blaOXA gene, and only one Campylobacter isolate carried several resistance genes. We showed that Campylobacter prevalence and the occurrence of antimicrobial resistance genes in the isolates is low in Finnish slaughter turkeys. Further, we demonstrated that Campylobacter spread between the flocks on the same farm, but the same STs did not persist.

弯曲杆菌是人类细菌性食源性胃肠道感染的主要原因，家禽是最重要的宿主之一。火鸡已被证明经常携带弯曲杆菌，从火鸡中分离出来的菌株显示出高度的抗菌素耐药性。我们利用芬兰一家火鸡屠宰场的监测数据，研究了2013-2023年芬兰屠宰火鸡中弯曲杆菌的流行情况，该数据涵盖了监测期间来自41个农场的1856只火鸡。此外，我们使用全基因组测序技术鉴定了2013年至2021年间从屠宰场收集的火鸡粪便样本中分离出的103株弯曲杆菌的细菌种类、耐药性谱、序列类型和核心基因组等位基因谱。共有9.5%的鸡群和82.9%的养殖场呈弯曲杆菌阳性。不同年份、月份和农场的患病率差异显著。以空肠弯曲杆菌（87.6%）最多，其次是大肠弯曲杆菌（4.0%）和lari弯曲杆菌（1.1%）。共获得34种序列类型，其中以ST45最为常见（占20.2%）。遗传上密切相关的分离株大多起源于同一农场，具有紧密的时间联系。鉴定出5种抗菌素基因型耐药谱。大多数弯曲杆菌只携带blaOXA基因，只有一株弯曲杆菌携带多个耐药基因。我们发现芬兰屠宰火鸡中弯曲杆菌的流行率和抗微生物药物耐药性基因的分离物发生率很低。此外，我们证明弯曲杆菌在同一农场的鸡群之间传播，但相同的STs并没有持续存在。

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