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Factors affecting consumer preference for healthy diet and functional foods 影响消费者对健康饮食和功能食品偏好的因素
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-04 DOI: 10.21603/2308-4057-2023-2-576
Slavica Grujić, Mirjana Grujčić
An inadequate diet and lifestyle are major causes of various diseases. A healthy diet can prevent diseases and maintain a good health status. The present research objective was to test young consumers for their preference for healthy diets and their intention to purchase functional foods, as well as to identify factors that affect their attitudes. The study involved 720 consumers (average 20 years old), a structured questionnaire, and a specially-developed scientific methodology. The results were grouped based on sex and body mass index. The analysis focused on the points where consumers’ interest in healthy diet overlapped with health-related diet needs and how each of them corresponded with their knowledge on the following spheres: nutrition and health risks, labelled data use in food choice, preference for foods fortified with vitamins, minerals, dietary fibers, or functional foods with a poor sensory quality. The data were processed using descriptive statistics, Z-test, and correlation tests. All the consumers demonstrated healthy diet preferences and a significant positive relationship (p < 0.01) with the factors. They made an informed choice based on the labelled data and preferred foods fortified with vitamins, minerals, and dietary fibers, as well as functional products with a lower sensory quality. All respondents, regardless of sex and body mass index, demonstrated nutrition and health risk awareness. The health-related diet needs also affected their food preferences (p < 0.05), with some exceptions. The young consumers possessed sufficient basic knowledge on food quality, nutrition, and health. Their attitudes depended on their preference for healthy diets and functional foods. The novel methodology can be applied to other studies of consumer preferences.
不适当的饮食和生活方式是导致各种疾病的主要原因。健康的饮食可以预防疾病,保持良好的健康状态。目前的研究目的是测试年轻消费者对健康饮食的偏好和购买功能食品的意愿,并确定影响他们态度的因素。该研究涉及720名消费者(平均年龄为20岁),采用结构化问卷调查和专门开发的科学方法。结果根据性别和体重指数进行分组。分析的重点是消费者对健康饮食的兴趣与与健康有关的饮食需求重叠的点,以及他们在以下领域的知识是如何对应的:营养和健康风险、食品选择中的标签数据使用、对维生素、矿物质、膳食纤维强化食品的偏好或感官质量较差的功能性食品。采用描述性统计、z检验和相关检验对数据进行处理。消费者均表现出健康饮食偏好,且与各因素呈显著正相关(p < 0.01)。他们根据标签上的数据做出了明智的选择,并选择了富含维生素、矿物质和膳食纤维的食物,以及感官质量较低的功能性产品。所有受访者,无论性别和体重指数如何,都表现出营养和健康风险意识。与健康相关的饮食需求也会影响他们的食物偏好(p < 0.05),但有一些例外。年轻消费者对食品质量、营养和健康有足够的基础知识。他们的态度取决于他们对健康饮食和功能食品的偏好。这种新方法可以应用于其他消费者偏好的研究。
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引用次数: 5
Plant extracts and essential oils in the dairy industry: A review 乳制品工业中的植物提取物和精油:综述
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-04 DOI: 10.21603/2308-4057-2023-2-579
Mahmoud Abd El-Aziz, H. Salama, Rehab S. Sayed
Plants have been used as food additives worldwide to enhance the sensory qualities of foods and extend their shelf life by reducing or eliminating foodborne pathogens. They also serve as therapeutic agents due to their beneficial effects on human health through their anti-cancerous, anti-inflammatory, antioxidant, and immune-modulatory properties. Plants can be added to food as a dry powder, grated material, paste, juice, or as an extract that can be produced by a variety of methods. Plant extracts and essential oils are concentrated sources of bioactive phytochemicals that can be added to food in small amounts in a variety of forms. These forms include liquid, semi-solid, or dry powder for easy and uniform diffusion. Encapsulation can protect bioactive compounds from temperature, moisture, oxidation, and light, as well as allow for controlling the release of the encapsulated ingredients. Nanoemulsions can enhance the bioactivity of active components. This review explains how plant extracts and essential oils are used in the dairy industry as antimicrobial materials, analyzing their impact on starter bacteria; as natural antioxidants to prevent the development of off-flavors and increase shelf life; and as technological auxiliaries, like milk-clotting enzymes, stabilizers, and flavoring agents. Therefore, plant extracts and essential oils are a better choice for the dairy industry than plants or their parts due to a wide range of applications, homogeneous dispersion, and ability to control the concentration of the bioactive ingredients and enhance their efficiency.
在世界范围内,植物已被用作食品添加剂,通过减少或消除食源性病原体来提高食品的感官品质并延长其保质期。由于其抗癌、抗炎、抗氧化和免疫调节特性对人体健康有益,它们也被用作治疗剂。植物可以作为干粉、磨碎的材料、糊状物、果汁或通过各种方法生产的提取物添加到食品中。植物提取物和精油是生物活性植物化学物质的浓缩来源,可以以各种形式少量添加到食物中。这些形式包括液体、半固体或干粉,便于均匀扩散。包封可以保护生物活性化合物不受温度、水分、氧化和光照的影响,也可以控制包封成分的释放。纳米乳可以提高活性成分的生物活性。本文介绍了植物提取物和精油作为抗菌材料在乳制品工业中的应用,分析了它们对发酵菌的影响;作为天然抗氧化剂,防止变质,延长保质期;作为技术助剂,比如凝乳酶、稳定剂和调味剂。因此,与植物或植物的部分相比,植物提取物和精油的应用范围广,分散均匀,能够控制生物活性成分的浓度并提高其效率,是乳制品行业更好的选择。
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引用次数: 3
Effect of dihydroquercetin on the toxic properties of nickel nanoparticles 二氢槲皮素对纳米镍毒性的影响
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-04 DOI: 10.21603/2308-4057-2023-2-572
I. Gmoshinski, M. Ananyan, V. Shipelin, N. Riger, E. Trushina, O. Mustafina, G. Guseva, A. Balakina, A. Kolobanov, S. Khotimchenko, D. Ozherelkov
Dihydroquercetin (3,5,7,3',4'-pentahydroxy-flavanone) is known for its powerful antioxidant, organ-protective, and antiinflammatory activities that can be applied to heavy-metal intoxication. The present research objective was to evaluate the possible protective potential of dietary dihydroquercetin in a rat model of subacute (92 days) intoxication with nickel nanoparticles. The experiment involved five groups of twelve male Wistar rats in each. Group 1 served as control. Other groups received nickel nanoparticles as part of their diet. Groups 2 and 4 received nickel nanoparticles with an average diameter of 53.7 nm (NiNP1), while groups 3 and 5 were fed with nanoparticles with an average diameter of 70.9 nm (NiNP2). The dose was calculated as 10 mg/kg b.w. Groups 4 and 5 also received 23 mg/kg b.w. of water-soluble stabilized dihydroquercetin with drinking water. After the dihydroquercetin treatment, the group that consumed 53.7 nm nickel nanoparticles demonstrated lower blood serum glucose, triglycerides, low-density lipoprotein cholesterol, and creatinine. Dihydroquercetin prevented the increase in total protein and albumin fraction associated with nickel nanoparticles intake. The experimental rats also demonstrated lower levels of pro-inflammatory cytokines IL-1β, IL-4, IL-6, and IL-17A, as well as a lower relative spleen weight after the treatment. In the group exposed to 53.7 nm nickel nanoparticles, the dihydroquercetin treatment increased the ratio of cytokines IL-10/IL-17A and decreased the level of circulating FABP2 protein, which is a biomarker of increased intestinal barrier permeability. In the group that received 70.9 nm nickel nanoparticles, the dihydroquercetin treatment inhibited the expression of the fibrogenic Timp3 gene in the liver. In the group that received 53.7 nm nickel nanoparticles, dihydroquercetin partially improved the violated morphology indexes in liver and kidney tissue. However, dihydroquercetin restored neither the content of reduced glutathione in the liver nor the indicators of selenium safety, which were suppressed under the effect of nickel nanoparticles. Moreover, the treatment failed to restore the low locomotor activity in the elevated plus maze test. Dihydroquercetin treatment showed some signs of detoxication and anti-inflammation in rats subjected to nickel nanoparticles. However, additional preclinical studies are necessary to substantiate its prophylactic potential in cases of exposure to nanoparticles of nickel and other heavy metals.
二氢槲皮素(3,5,7,3',4'-五羟基黄酮)以其强大的抗氧化、器官保护和抗炎活性而闻名,可用于重金属中毒。本研究的目的是评估膳食二氢槲皮素对镍纳米颗粒亚急性(92天)中毒大鼠模型的保护作用。实验涉及五组,每组12只雄性Wistar大鼠。第一组为对照组。其他组的饮食中含有镍纳米颗粒。2、4组平均直径53.7 nm (NiNP1), 3、5组平均直径70.9 nm (NiNP2)。按10 mg/kg b.w.计算剂量,第4组和第5组同时饮水给予水溶性稳定双氢槲皮素23 mg/kg b.w.。在二氢槲皮素治疗后,摄入53.7 nm镍纳米颗粒的组表现出较低的血清葡萄糖、甘油三酯、低密度脂蛋白胆固醇和肌酐。二氢槲皮素阻止了总蛋白和白蛋白含量的增加,这些增加与镍纳米颗粒的摄入有关。实验大鼠的促炎细胞因子IL-1β、IL-4、IL-6和IL-17A水平降低,脾脏相对重量降低。在53.7 nm镍纳米颗粒暴露组中,二氢槲皮素处理增加了细胞因子IL-10/IL-17A的比例,降低了循环FABP2蛋白的水平,这是肠道屏障通透性增加的生物标志物。在接受70.9 nm镍纳米颗粒的组中,二氢槲皮素处理抑制了肝脏中纤维化Timp3基因的表达。在53.7 nm镍纳米颗粒组,二氢槲皮素部分改善了肝脏和肾脏组织的形态学指标。然而,二氢槲皮素并没有恢复肝脏中还原性谷胱甘肽的含量,也没有恢复硒的安全性指标,这些指标在纳米镍的作用下受到抑制。此外,在升高+迷宫测试中,治疗未能恢复低运动活动。二氢槲皮素处理对镍纳米颗粒大鼠有一定的解毒和抗炎作用。然而,需要进一步的临床前研究来证实其在接触镍纳米粒子和其他重金属的情况下的预防潜力。
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引用次数: 1
Effects of hydrolysis degree on the functional properties of hydrolysates from sour cherry kernel protein concentrate 水解度对酸樱桃仁浓缩蛋白水解产物功能性质的影响
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-04 DOI: 10.21603/2308-4057-2023-2-566
A. Cingöz, M. Yıldırım
During the processing of sour cherries into different foodstuffs, a large amount of kernels is produced as waste material, which creates a significant disposal problem for the food industry. Sour cherry kernels containing 25.3–35.5% of protein can be used as a functional protein source in food production. Therefore, we aimed to study the effects of hydrolysis degree on the sour cherry kernel protein hydrolysates. Proteins were extracted from the defatted flour by isoelectric precipitation. The resulting protein concentrate was hydrolyzed (5, 10, and 15% hydrolysis) using Alcalase to yield hydrolysates. We determined their oil and water holding, emulsifying, gelation, and foaming properties, as well as apparent molecular weight distribution and proximate compositions. No protein fractions greater than an apparent molecular weight of about 22 kDa were present in the hydrolysates. The hydrolysis of the protein concentrate mostly led to an increase in protein solubility. As the degree of hydrolysis increased from 5 to 15%, the water holding capacity of the hydrolysates decreased from 2.50 ± 0.03 to 2.03 ± 0.02 g water/g, indicating its deterioration. The hydrolysates obtained at different degrees of hydrolysis had a better solubility than the intact protein concentrate. The oil holding capacity, the foaming stability, and the least gelation concentration of the protein concentrate could not be considerably improved by hydrolysis. In contrast, its emulsifying activity index and foaming capacity could be increased with a limited degree of hydrolysis (up to 10%).
在将酸樱桃加工成不同食品的过程中,会产生大量的果仁作为废料,这给食品行业带来了重大的处理问题。酸樱桃仁含有25.3-35.5%的蛋白质,可作为食品生产中的功能性蛋白质来源。因此,我们旨在研究水解度对酸樱桃仁蛋白水解产物的影响。通过等电沉淀法从脱脂面粉中提取蛋白质。使用Alcalase将所得蛋白浓缩物水解(5%、10%和15%水解)以产生水解产物。我们测定了它们的保油性、保水性、乳化性、胶凝性和发泡性,以及表观分子量分布和近似组成。水解产物中不存在大于约22kDa的表观分子量的蛋白质级分。蛋白质浓缩物的水解主要导致蛋白质溶解度的增加。当水解度从5%增加到15%时,水解产物的持水能力从2.50±0.03克水/g降低到2.03±0.02克水/g,表明其劣化。在不同水解度下获得的水解产物比完整的蛋白质浓缩物具有更好的溶解度。水解不能显著提高蛋白质浓缩物的含油量、发泡稳定性和最小凝胶浓度。相反,它的乳化活性指数和发泡能力可以在有限的水解度(高达10%)下提高。
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引用次数: 0
Effect of cocoa husk Criollo tea on hypercholesterolemia in animal model 可可皮茶对高胆固醇血症动物模型的影响
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-04 DOI: 10.21603/2308-4057-2023-2-567
A. Adi, Ali Iqbal Tawakal, Mohammad Fahmi Rasyidi, Wizara Salisa, Farapti Farapti, H. Rachmawati
Organic waste is a problem the cocoa industry has to handle. The industry produces a lot of cocoa bean husk, also called criollo cocoa husk. Cocoa bean husk is an underutilized cocoa waste that contains bioactive components in the form of phenols and flavonoids. Processed cocoa bean husk can be brewed as a functional beverage. The research objective was to test cocoa husk tea for sensory properties, bioactive components, and impact on blood cholesterol. This study used a randomized experimental design with six repetitions. Sensory data were processed using the Friedman and Wilcoxon signed-rank tests (α = 0.05) to determine the difference in sensory properties between each formulation of cocoa husk tea. The sensory evaluation involved 30 untrained panelists who gave the highest score to the formulation with 62.5% cocoa bean husk, 25% lemongrass, and 12.5% aromatic ginger, which could also reduce 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals (IC50 = 264.8675). The animal test showed that the cocoa husk formulation produced no significant difference (p > 0.05) in pre- and post-treatment, but was able to keep cholesterol within normal limits. Cocoa bean husk showed health benefits by its antioxidant properties and ability to control blood cholesterol.
有机废物是可可工业必须处理的一个问题。该行业生产大量可可豆壳,也称为克里奥洛可可壳。可可豆壳是一种未充分利用的可可废料,含有酚类和黄酮类生物活性成分。经过加工的可可豆壳可以作为一种功能饮料酿造。研究目的是测试可可皮茶的感官特性、生物活性成分以及对血液胆固醇的影响。本研究采用随机实验设计,共6次重复。使用Friedman和Wilcoxon符号秩检验(α=0.05)对感官数据进行处理,以确定每种可可皮茶配方之间感官特性的差异。感官评估涉及30名未经培训的小组成员,他们对含有62.5%可可豆壳、25%柠檬草和12.5%芳香姜的配方给予了最高的分数,该配方还可以减少2,2-二苯基-1-苦基肼(DPPH)自由基(IC50=264.8675)。动物试验表明,可可壳配方在治疗前后没有产生显著差异(p>0.05),但能够将胆固醇保持在正常范围内。可可豆壳具有抗氧化特性和控制血液胆固醇的能力,对健康有益。
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引用次数: 2
Hyptis suaveolens L. leaf extracts in traditional health care systems 传统保健体系中的蛇耳草叶提取物
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-04 DOI: 10.21603/2308-4057-2023-2-577
F. Akharaiyi, C. B. Ehis-Eriakha, P. T. Olagbemide, Faith Hukwu Igbudu
Hyptis suaveolens L. is a medical and food plant that is commonly used to treat various microbial infections in humans in many countries of the world. We aimed to study the aqueous and ethanol extracts of H. suaveolens leaves to determine their antibacterial, in-vitro antioxidant, and phytochemical potentials for traditional medicine by using chemical analysis. The aqueous and ethanol extracts inhibited the tested bacteria species with zones of 0–15 and 10–29 mm, respectively. On the typed culture isolates, the inhibition zones were 8–25 and 16–32 mm for the aqueous and ethanol extracts, respectively. The minimum inhibitory concentrations of the aqueous and ethanol extracts were not different, while the minimum bactericidal concentrations for the aqueous extract was higher than that for the ethanol extract. The screened phytochemicals were qualitatively and quantitatively present in both extracts, except for saponins which were absent in the aqueous extract. The free radical scavenging activity in the aqueous and ethanol extracts was 1.44 ± 0.50 and 1.57 ± 1.40 mg of ascorbic acid/1 g dry leaves, respectively. The ferric reduction was 1.19 ± 0.40 and 1.69 ± 0.18 mg of ascorbic acid/1 g dry leaves in the aqueous and ethanol extracts, respectively. Hydroxyl scavenging was 65.0 ± 0.9 and 0.43 ± 0.50 mg of ascorbic acid/1 g dry leaves for the aqueous and ethanol extracts, respectively. The present research suggests that the extract of H. suaveleons can be applied as a controlling antibacterial growth agent against Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Streptococcus pneumoniae and other bacterial pathogens. It is noteworthy that the ethanol extract was more effective than the aqueous one in terms of the antibacterial, phytochemical and antioxidant activities.
猪耳草是一种药用和食用植物,在世界上许多国家常用来治疗人类的各种微生物感染。本研究采用化学分析的方法,对水提物和乙醇提物进行研究,以确定其抑菌活性、体外抗氧化活性和传统药物的植物化学活性。水提物和乙醇提物对细菌的抑制范围分别为0 ~ 15 mm和10 ~ 29 mm。对分型培养菌株,水提液和乙醇提液的抑制区分别为8 ~ 25 mm和16 ~ 32 mm。水提物和乙醇提物的最低抑菌浓度差异不大,但水提物的最低杀菌浓度高于乙醇提物。筛选的植物化学物质定性和定量地存在于两种提取物中,除了水提取物中不存在的皂苷。抗坏血酸含量为1.44±0.50 mg / g,抗坏血酸含量为1.57±1.40 mg / g。水提液和乙醇提液的铁还原量分别为1.19±0.40和1.69±0.18 mg / g干叶。水提液和乙醇提液对抗坏血酸的清除率分别为65.0±0.9和0.43±0.50 mg /1 g干叶。本研究表明,该提取物可作为铜绿假单胞菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、肺炎链球菌等病原菌的抑菌生长剂。值得注意的是,乙醇提取物在抗菌、植物化学和抗氧化活性方面比水提取物更有效。
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引用次数: 0
Use of animal origin protein concentrates in bread baking 动物源性浓缩蛋白在面包烘焙中的应用
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-04-04 DOI: 10.21603/2308-4057-2023-2-570
Maria Shabunina, Anastasiya Andreeva, Anastasia Pavlova
Protein deficiency in the human diet is a widespread problem that affects all body systems. Nutrition adjustment appears to be one of the most effective ways to prevent this problem. This study was aimed at investigating the possibilities of using animal origin protein concentrates in bread baking industry. Study objects included five breads: one control and four samples containing protein concentrates. The test wheat bread samples contained 7, 9, and 11% of milk protein concentrate and 7% of whey protein concentrate to the mass of flour in the dough. Rheological parameters of the dough were obtained using an alveograph and a farinograph. The specific volume and sensory characteristics of the baked products as well as crumb deformation were evaluated. The nutritional value of two samples – control and with 9% of milk protein concentrate addition – was determined by calculation. Whey protein concentrate had a negative influence on the dough rheology. Low water binding capacity and specific volume as well as hard crumb make the usage of this product in bread baking unacceptable. The palatability test showed that bread supplemented with 9% of milk protein concentrate had the best sensory characteristics, compared to control and other test samples (with 7 and 11% of this concentrate). The predicted protein content was equal to 11.6 g/100 g that is 35% higher than in the control sample. Consequently, milk protein concentrate has a potential to improve biological value of the bread that can be further helpful to prevent the protein deficiency. Milk protein concentrate can be successfully used in the bread making process as an ingredient to correct the nutritional and biological value of baked products.
人类饮食中的蛋白质缺乏是一个影响所有身体系统的普遍问题。营养调整似乎是预防这一问题的最有效方法之一。本研究旨在探讨在面包烘焙工业中使用动物源性浓缩蛋白的可能性。研究对象包括五个面包:一个对照和四个含有浓缩蛋白的样品。相对于面团中的面粉质量,测试小麦面包样品含有7%、9%和11%的牛奶浓缩蛋白和7%的乳清浓缩蛋白。面团的流变参数是使用淀粉描记器和粉质描记器获得的。对烘焙产品的比体积和感官特性以及面包屑变形进行了评估。通过计算确定了两个样品的营养价值——对照和添加9%牛奶浓缩蛋白。乳清蛋白浓缩物对面团流变性有负面影响。低的水分结合能力和比体积以及坚硬的面包屑使得该产品在面包烘焙中的使用是不可接受的。适口性测试表明,与对照和其他测试样品(含7%和11%的浓缩乳蛋白)相比,添加9%浓缩乳蛋白的面包具有最佳的感官特性。预测的蛋白质含量等于11.6g/100g,比对照样品中的蛋白质含量高35%。因此,牛奶浓缩蛋白具有提高面包生物价值的潜力,这可以进一步有助于预防蛋白质缺乏。牛奶浓缩蛋白可以成功地用于面包制作过程中,作为一种配料来纠正烘焙产品的营养和生物价值。
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引用次数: 2
Agriculture in the Baksan Gorge of the Central Caucasus, Kabardino-Balkaria, Russia 俄罗斯卡巴尔达-巴尔卡里亚,高加索中部巴克桑峡谷的农业
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2023-03-14 DOI: 10.21603/2308-4057-2023-1-561
E. Abakumov, R. Tembotov
No agriculture is possible without soil. This article reviews available data on the soils of the Baksan Gorge located in the Kabardino-Balkarian Republic, Russia. The research objective was to collect and analyze information on the soil composition and crop yields in this region of the Central Caucasus. The review covered the last five years of scientific publications cited in Scopus, Web of Science, and Elibrary. It also featured contemporary and archival documents on the soil composition and periglacial agriculture in the Baksan Gorge. The agriculture and cattle breeding started in the Central Caucasus in the first millennium BC when the local peoples began to develop these lands as highland pastures and, subsequently, for agricultural farming. During the second millennium BC, crop production became one of the most important economic sectors in the Central Caucasus. Corn, barley, wheat, and millet were the main agricultural crops in the Baksan Gorge. Millet has always been a traditional Kabardian crop, and millet farming occupied the largest flatland areas. Barley was the staple crop in the highlands. Currently, the list of local staple crops includes corn, wheat, and sunflower. Barley, oats, peas, potatoes, vegetables, berries, nuts, grapes, and annual herbs are also popular. The past fifteen years have seen an extensive development of intensive horticulture in the Baksan Gorge. Agricultural ecology and production problems depend on the localization of agriculture in the Central Caucasus. This research reviewed data on the effect of soil composition on the yield and value of agricultural crops in the Baksan Gorge of the Central Caucasus.
没有土壤就不可能有农业。本文回顾了位于俄罗斯卡巴尔迪尼奥·巴尔卡里亚共和国的巴克桑峡谷土壤的现有数据。研究目的是收集和分析有关中高加索地区土壤成分和作物产量的信息。该综述涵盖了Scopus、Web of Science和Elibrary引用的过去五年的科学出版物。它还收录了关于巴克桑峡谷土壤成分和冰缘农业的当代和档案文件。农业和养牛始于公元前一千年的中高加索地区,当时当地人开始将这些土地开发为高地牧场,随后用于农业。在公元前第二个千年期间,作物生产成为中高加索地区最重要的经济部门之一。玉米、大麦、小麦和小米是巴桑峡谷的主要农业作物。小米一直是卡巴迪的传统作物,小米种植占据了最大的平原地区。大麦是高地的主要作物。目前,当地主要作物包括玉米、小麦和向日葵。大麦、燕麦、豌豆、土豆、蔬菜、浆果、坚果、葡萄和一年生草本植物也很受欢迎。在过去的十五年里,巴克桑峡谷的集约园艺得到了广泛的发展。农业生态和生产问题取决于中高加索地区农业的本地化。本研究回顾了中高加索巴克桑峡谷土壤成分对农作物产量和价值影响的数据。
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引用次数: 1
Embryogenesis induction of carrot (Daucus carota L.) in isolated microspore culture 胡萝卜(Daucus carota L.)离体小孢子培养中胚胎发生的诱导
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-10-17 DOI: 10.21603/2308-4057-2023-1-548
O. Romanova, T. Vjurtts, A. Mineykina, Yana Tukuser, Yu.S. Kulakov, V. Akhramenko, A. Soldatenko, E. Domblides
Haploid technologies are used to create homozygous lines for accelerated breeding. We aimed to optimize the technology for using the isolated microspore culture in vitro to obtain doubled haploids of the carrot (Daucus carota L.). We studied two carrot varieties with different responsiveness to embryogenesis, Altajskaya lakomka and Breeding line 17. Carrot microspores were isolated from buds and cultivated in liquid nutrient media supplemented with an antibiotic and activated carbon in vitro. They were exposed to different thermal treatments. The experiment showed the benefits of combining cold pre-treatment of buds (5°C for 1 day) with heat shock of isolated microspores in vitro (32°C for 2 days). The induction of embryogenesis on the NLN-13 medium was twice as high as on the MSm-13 medium. The use of 1% activated carbon in 0.5% agarose increased the yield of embryoids by more than 1.5 times. 100 mg/dm3 of ampicillin was found to be the most efficient concentration. After 30 days of cultivation under optimized conditions, the yield was 161.3 and 44.0 embryoids per Petri dish for the cultivar Altajskaya lakomka and Breeding line 17, respectively. The induction of carrot embryogenesis is determined by the type and duration of thermal stress, the composition of the nutrient medium, the use of activated carbon as a sorbent, the addition of β-lactam antibiotics, and the type of explant exposed to thermal treatment. Our technology enabled us to obtain homozygous doubled haploid lines of carrots during a year, and these lines were included in the breeding process to create F1 hybrids.
单倍体技术被用来创造用于加速育种的纯合系。以胡萝卜(Daucus carota L.)为材料,研究了两个对胚胎发生反应不同的胡萝卜品种Altajskaya lakomka和17号品系。从芽中分离胡萝卜小孢子,并在添加抗生素和活性炭的液体营养培养基中进行体外培养。他们接受了不同的热处理。实验表明,芽的冷预处理(5°C,1天)与离体小孢子的热休克(32°C,2天)相结合是有益的。在NLN-13培养基上对胚胎发生的诱导是在MSm-13培养基上的两倍高。在0.5%琼脂糖中使用1%活性炭使胚状体的产量增加了1.5倍以上。发现100mg/dm3的氨苄青霉素是最有效的浓度。在优化条件下培养30天后,栽培品种Altajskaya lakomka和育种系17的每皮氏培养皿产量分别为161.3和44.0胚。胡萝卜胚胎发生的诱导取决于热胁迫的类型和持续时间、营养培养基的组成、活性炭作为吸附剂的使用、β-内酰胺抗生素的添加以及暴露于热处理的外植体的类型。我们的技术使我们能够在一年内获得纯合的胡萝卜双单倍体系,这些系被纳入育种过程,以创造F1杂交种。
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引用次数: 2
Evaluating extremophilic microorganisms in industrial regions 工业区极端微生物的评价
IF 1 Q4 FOOD SCIENCE & TECHNOLOGY Pub Date : 2022-10-17 DOI: 10.21603/2308-4057-2023-1-556
L. Asyakina, Ekaterina Vorob’eva, L. Proskuryakova, M. Zharko
Abiotic and biotic stresses have a major impact on crop growth. Stress affects the root system and decreases the amount of nutrients in fruits. Modern agricultural technologies help replace mineral fertilizers with new generation biopreparation. Unlike chemical fertilizers, biofertilizers reduce the risk of adverse environmental impacts. Of special interest are extremophilic microorganisms able to survive in extreme conditions. We aimed to study the phytostimulating ability of extremophilic bacteria isolated from disturbed lands in the coal-mining region. We isolated microorganisms from disturbed lands and studied their cultural, morphological, and biochemical properties. Then, we determined their ability to synthesize indole-3-acetic acids. The extremophilic bacteria were identified and subjected to biocompatibility testing by co-cultivation. Next, we created consortia of pure cultures and analyzed biomass growth. Finally, the biopreparation was experimentally tested on Trifolium prantense L. seeds. We isolated 10 strains of microorganisms that synthesized 4.39 to 16.32 mg/mL of indole-3-acetic acid. The largest amounts of the acid were produced by Pantoea spp., Enterococcus faecium, Leclercia spp., Rothia endophytica, and Klebsiella oxytoca. A consortium of Pantoea spp., E. faecium, and R. endophytica at a ratio of 1:1:1 produced the largest amount of indole-3-acetic acid (15.59 mg/mL) and accumulated maximum biomass. The addition of 0.2% L-tryptophan to the nutrient medium increased the amount of indole-3-acetic acid to 18.45 mg/mL. When the T. prantense L. seeds were soaked in the biopreparation (consortium’s culture fluid) at a concentration of 2.5, the sprouts were 1.4 times longer on the 10th day of growth, compared to the control. The consortium of Pantoea spp., E. faecium, and R. endophytica (1:1:1) stimulated the growth of T. prantense L. seeds. Our findings can be further used to develop biofertilizers for agriculture.
非生物胁迫和生物胁迫对作物生长有重要影响。压力会影响根系,减少水果中的营养成分。现代农业技术有助于用新一代生物修复取代矿物肥料。与化肥不同,生物肥料降低了对环境造成不利影响的风险。特别令人感兴趣的是能够在极端条件下生存的极端微生物。本研究旨在研究从煤矿区扰动土地上分离的极端微生物的植物刺激能力。我们从扰动的土地上分离出微生物,并研究了它们的培养、形态和生化特性。然后,我们测定了它们合成吲哚-3-乙酸的能力。对极端嗜微生物进行了鉴定,并通过共培养进行了生物相容性测试。接下来,我们创建了纯文化联盟,并分析了生物量的增长。最后,在三叶草种子上进行了生物修复试验。我们分离了10株微生物,它们合成了4.39至16.32mg/mL的吲哚-3-乙酸。产酸量最大的是泛球菌属、粪肠球菌属、勒克莱尔菌属、内生Rothia和氧化克雷伯菌。Pantoea spp.、E.faceium和R.内生菌以1:1:1的比例形成的群落产生了最大量的吲哚-3-乙酸(15.59mg/mL),并积累了最大的生物量。向营养培养基中添加0.2%的L-色氨酸使吲哚-3-乙酸的量增加到18.45mg/mL。当将T.pranense L.种子浸泡在浓度为2.5的生物制剂(财团培养液)中时,与对照相比,芽在生长的第10天长了1.4倍。Pantea spp.、E.faceium和R.内生菌(1:1:1)的联合体刺激了T.pratense L.种子的生长。我们的发现可以进一步用于开发农业生物肥料。
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引用次数: 2
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Foods and Raw Materials
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