Brijesh K Mehta, Hema S Chauhan, Sudipta Basu, Anjali Anand, Aanchal Baveja, Rajkumar U Zunjare, Vignesh Muthusamy, Ashok K Singh, Firoz Hossain
Sweet corn is one of the most popular vegetables worldwide. However, traditional shrunken2 (sh2 )-based sweet corn varieties are poor in nutritional quality. Here, we analysed the effect of (1) β-carotene hydroxylase1 (crtRB1 ), (2) opaque2 (o2 ) and (3) o2+crtRB1 genes on nutritional quality, germination, seed vigour and physico-biochemical traits in a set of 27 biofortified sh2 -based sweet corn inbreds. The biofortified sweet corn inbreds recorded significantly higher concentrations of proA (16.47μg g-1 ), lysine (0.36%) and tryptophan (0.09%) over original inbreds (proA: 3.14μg g-1 , lysine: 0.18%, tryptophan: 0.04%). The crtRB1 -based inbreds had the lowest electrical conductivity (EC), whereas o2 -based inbreds possessed the highest EC. The o2 +crtRB1 -based inbreds showed similar EC to the original inbreds. Interestingly, o2 -based inbreds also had the lowest germination and seed vigour compared to original inbreds, whereas crtRB1 and o2 +crtRB1 introgressed sweet corn inbreds showed similar germination and seed vigour traits to their original versions. This suggested that the negative effect of o2 on germination, seed vigour and EC is nullified by crtRB1 in the double mutant sweet corn. Overall, o2 +crtRB1 -based sweet corn inbreds were found the most desirable over crtRB1 - and o2 -based inbreds alone.
{"title":"Mutant <i>crtRB1</i> gene negates the unfavourable effects of <i>opaque2</i> gene on germination and seed vigour among <i>shrunken2</i>-based biofortified sweet corn genotypes.","authors":"Brijesh K Mehta, Hema S Chauhan, Sudipta Basu, Anjali Anand, Aanchal Baveja, Rajkumar U Zunjare, Vignesh Muthusamy, Ashok K Singh, Firoz Hossain","doi":"10.1071/FP23179","DOIUrl":"10.1071/FP23179","url":null,"abstract":"<p><p>Sweet corn is one of the most popular vegetables worldwide. However, traditional shrunken2 (sh2 )-based sweet corn varieties are poor in nutritional quality. Here, we analysed the effect of (1) β-carotene hydroxylase1 (crtRB1 ), (2) opaque2 (o2 ) and (3) o2+crtRB1 genes on nutritional quality, germination, seed vigour and physico-biochemical traits in a set of 27 biofortified sh2 -based sweet corn inbreds. The biofortified sweet corn inbreds recorded significantly higher concentrations of proA (16.47μg g-1 ), lysine (0.36%) and tryptophan (0.09%) over original inbreds (proA: 3.14μg g-1 , lysine: 0.18%, tryptophan: 0.04%). The crtRB1 -based inbreds had the lowest electrical conductivity (EC), whereas o2 -based inbreds possessed the highest EC. The o2 +crtRB1 -based inbreds showed similar EC to the original inbreds. Interestingly, o2 -based inbreds also had the lowest germination and seed vigour compared to original inbreds, whereas crtRB1 and o2 +crtRB1 introgressed sweet corn inbreds showed similar germination and seed vigour traits to their original versions. This suggested that the negative effect of o2 on germination, seed vigour and EC is nullified by crtRB1 in the double mutant sweet corn. Overall, o2 +crtRB1 -based sweet corn inbreds were found the most desirable over crtRB1 - and o2 -based inbreds alone.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139704421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Guillaume Tcherkez, Sinda Ben Mariem, Iván Jauregui, Luis Larraya, Jose M García-Mina, Angel M Zamarreño, Andreas Fangmeier, Iker Aranjuelo
While the effect of CO2 enrichment on wheat (Triticum spp.) photosynthesis, nitrogen content or yield has been well-studied, the impact of elevated CO2 on metabolic pathways in organs other than leaves is poorly documented. In particular, glumes and awns, which may refix CO2 respired by developing grains and be naturally exposed to higher-than-ambient CO2 mole fraction, could show specific responses to elevated CO2 . Here, we took advantage of a free-air CO2 enrichment experiment and performed multilevel analyses, including metabolomics, ionomics, proteomics, major hormones and isotopes in Triticum durum . While in leaves, elevated CO2 tended to accelerate amino acid metabolism with many significantly affected metabolites, the effect on glumes and awns metabolites was modest. There was a lower content in compounds of the polyamine pathway (along with uracile and allantoin) under elevated CO2 , suggesting a change in secondary N metabolism. Also, cytokinin metabolism appeared to be significantly affected under elevated CO2 . Despite this, elevated CO2 did not affect the final composition of awn and glume organic matter, with the same content in carbon, nitrogen and other elements. We conclude that elevated CO2 mostly impacts on leaf metabolism but has little effect in awns and glumes, including their composition at maturity.
虽然二氧化碳富集对小麦(Triticum spp.)光合作用、氮含量或产量的影响已得到充分研究,但高浓度二氧化碳对叶片以外器官代谢途径的影响却鲜有记载。特别是颖果和芒,它们可能会重新吸收发育中的谷粒呼吸的二氧化碳,并自然暴露在高于环境的二氧化碳摩尔分数下,可能会对升高的二氧化碳表现出特殊的反应。在这里,我们利用自由空气二氧化碳富集实验,对硬质小麦进行了多层次分析,包括代谢组学、离子组学、蛋白质组学、主要激素和同位素。在叶片中,高浓度 CO2 有加速氨基酸代谢的趋势,许多代谢物受到显著影响,但对颖果和芒果代谢物的影响不大。在二氧化碳升高的条件下,多胺途径化合物(以及尿囊素和尿囊素)的含量较低,这表明次生氮代谢发生了变化。此外,细胞分裂素的代谢在二氧化碳升高条件下似乎也受到了显著影响。尽管如此,高浓度 CO2 并没有影响芒和颖片有机物的最终组成,碳、氮和其他元素的含量相同。我们的结论是,高浓度二氧化碳主要影响叶片的新陈代谢,但对芒和颖片的影响很小,包括它们成熟时的组成。
{"title":"Differential effects of elevated CO<sub>2</sub> on awn and glume metabolism in durum wheat (<i>Triticum durum</i>).","authors":"Guillaume Tcherkez, Sinda Ben Mariem, Iván Jauregui, Luis Larraya, Jose M García-Mina, Angel M Zamarreño, Andreas Fangmeier, Iker Aranjuelo","doi":"10.1071/FP23255","DOIUrl":"10.1071/FP23255","url":null,"abstract":"<p><p>While the effect of CO2 enrichment on wheat (Triticum spp.) photosynthesis, nitrogen content or yield has been well-studied, the impact of elevated CO2 on metabolic pathways in organs other than leaves is poorly documented. In particular, glumes and awns, which may refix CO2 respired by developing grains and be naturally exposed to higher-than-ambient CO2 mole fraction, could show specific responses to elevated CO2 . Here, we took advantage of a free-air CO2 enrichment experiment and performed multilevel analyses, including metabolomics, ionomics, proteomics, major hormones and isotopes in Triticum durum . While in leaves, elevated CO2 tended to accelerate amino acid metabolism with many significantly affected metabolites, the effect on glumes and awns metabolites was modest. There was a lower content in compounds of the polyamine pathway (along with uracile and allantoin) under elevated CO2 , suggesting a change in secondary N metabolism. Also, cytokinin metabolism appeared to be significantly affected under elevated CO2 . Despite this, elevated CO2 did not affect the final composition of awn and glume organic matter, with the same content in carbon, nitrogen and other elements. We conclude that elevated CO2 mostly impacts on leaf metabolism but has little effect in awns and glumes, including their composition at maturity.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Minmin He, Gui Geng, Shuyang Mei, Gang Wang, Lihua Yu, Yao Xu, Yuguang Wang
Water stress (drought and waterlogging) leads to an imbalance in plant water distribution, disrupts cell homeostasis, and severely inhibits plant growth. Melatonin is a growth hormone that plants synthesise and has been shown to resist adversity in many plants. This review discusses the biosynthesis and metabolism of melatonin, as well as the changes in plant morphology and physiological mechanisms caused by the molecular defence process. Melatonin induces the expression of related genes in the process of plant photosynthesis under stress and protects the structural integrity of chloroplasts. Exogenous melatonin can maintain the dynamic balance of root ion exchange under waterlogging stress. Melatonin can repair mitochondria and alleviate damage caused by reactive oxygen species and reactive nitrogen species; and has a wide range of uses in the regulation of stress-specific genes and the activation of antioxidant enzyme genes. Melatonin improves the stability of membrane lipids in plant cells and maintains osmotic balance by regulating water channels. There is crosstalk between melatonin and other hormones, which jointly improve the ability of the root system to absorb water and breathe and promote plant growth. Briefly, as a multifunctional molecule, melatonin improves the tolerance of plants under water stress and promotes plant growth and development.
{"title":"Melatonin modulates the tolerance of plants to water stress: morphological response of the molecular mechanism.","authors":"Minmin He, Gui Geng, Shuyang Mei, Gang Wang, Lihua Yu, Yao Xu, Yuguang Wang","doi":"10.1071/FP23199","DOIUrl":"10.1071/FP23199","url":null,"abstract":"<p><p>Water stress (drought and waterlogging) leads to an imbalance in plant water distribution, disrupts cell homeostasis, and severely inhibits plant growth. Melatonin is a growth hormone that plants synthesise and has been shown to resist adversity in many plants. This review discusses the biosynthesis and metabolism of melatonin, as well as the changes in plant morphology and physiological mechanisms caused by the molecular defence process. Melatonin induces the expression of related genes in the process of plant photosynthesis under stress and protects the structural integrity of chloroplasts. Exogenous melatonin can maintain the dynamic balance of root ion exchange under waterlogging stress. Melatonin can repair mitochondria and alleviate damage caused by reactive oxygen species and reactive nitrogen species; and has a wide range of uses in the regulation of stress-specific genes and the activation of antioxidant enzyme genes. Melatonin improves the stability of membrane lipids in plant cells and maintains osmotic balance by regulating water channels. There is crosstalk between melatonin and other hormones, which jointly improve the ability of the root system to absorb water and breathe and promote plant growth. Briefly, as a multifunctional molecule, melatonin improves the tolerance of plants under water stress and promotes plant growth and development.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139734821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Christian Harrison, Clarice Noleto-Dias, Gianluca Ruvo, David J Hughes, Daniel P Smith, Andrew Mead, Jane L Ward, Sigrid Heuer, Dana R MacGregor
Black-grass (Alopecurus myosuroides ) is one of the most problematic agricultural weeds of Western Europe, causing significant yield losses in winter wheat (Triticum aestivum ) and other crops through competition for space and resources. Previous studies link black-grass patches to water-retaining soils, yet its specific adaptations to these conditions remain unclear. We designed pot-based waterlogging experiments to compare 13 biotypes of black-grass and six cultivars of wheat. These showed that wheat roots induced aerenchyma when waterlogged whereas aerenchyma-like structures were constitutively present in black-grass. Aerial biomass of waterlogged wheat was smaller, whereas waterlogged black-grass was similar or larger. Variability in waterlogging responses within and between these species was correlated with transcriptomic and metabolomic changes in leaves of control or waterlogged plants. In wheat, transcripts associated with regulation and utilisation of phosphate compounds were upregulated and sugars and amino acids concentrations were increased. Black-grass biotypes showed limited molecular responses to waterlogging. Some black-grass amino acids were decreased and one transcript commonly upregulated was previously identified in screens for genes underpinning metabolism-based resistance to herbicides. Our findings provide insights into the different waterlogging tolerances of these species and may help to explain the previously observed patchiness of this weed's distribution in wheat fields.
{"title":"The mechanisms behind the contrasting responses to waterlogging in black-grass (<i>Alopecurus myosuroides</i>) and wheat (<i>Triticum aestivum</i>).","authors":"Christian Harrison, Clarice Noleto-Dias, Gianluca Ruvo, David J Hughes, Daniel P Smith, Andrew Mead, Jane L Ward, Sigrid Heuer, Dana R MacGregor","doi":"10.1071/FP23193","DOIUrl":"10.1071/FP23193","url":null,"abstract":"<p><p>Black-grass (Alopecurus myosuroides ) is one of the most problematic agricultural weeds of Western Europe, causing significant yield losses in winter wheat (Triticum aestivum ) and other crops through competition for space and resources. Previous studies link black-grass patches to water-retaining soils, yet its specific adaptations to these conditions remain unclear. We designed pot-based waterlogging experiments to compare 13 biotypes of black-grass and six cultivars of wheat. These showed that wheat roots induced aerenchyma when waterlogged whereas aerenchyma-like structures were constitutively present in black-grass. Aerial biomass of waterlogged wheat was smaller, whereas waterlogged black-grass was similar or larger. Variability in waterlogging responses within and between these species was correlated with transcriptomic and metabolomic changes in leaves of control or waterlogged plants. In wheat, transcripts associated with regulation and utilisation of phosphate compounds were upregulated and sugars and amino acids concentrations were increased. Black-grass biotypes showed limited molecular responses to waterlogging. Some black-grass amino acids were decreased and one transcript commonly upregulated was previously identified in screens for genes underpinning metabolism-based resistance to herbicides. Our findings provide insights into the different waterlogging tolerances of these species and may help to explain the previously observed patchiness of this weed's distribution in wheat fields.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pengcheng Ding, Peng Tang, Xiaofen Li, Adeela Haroon, Saima Nasreen, Hafeez Noor, Kotb A Attia, Asmaa M Abushady, Rongzhen Wang, Kaiyuan Cui, Xiangyun Wu, Min Sun, Zhiqiang Gao
The MYB transcription factor (TF) are among the largest gene families of plants being responsible for several biological processes. The R2R3-MYB gene family are integral player regulating plant primary and secondary metabolism, growth and development, and responses to hormones and stresses. The phylogenetic analysis combined with gene structure analysis and motif determination resulted in division of R2R3-MYB gene family into 27 subgroups. Evidence generated from synteny analyses indicated that CqR2R3-MYBs gene family is featured by tandem and segmental duplication events. On the basis of RNA-Seq data, the expression patterns of different tissues under salt treatment were investigated resulting CqR2R3-MYB genes high expression both in roots and stem of quinoa (Chenopodium quinoa ) plants. More than half of CqR2R3-MYB genes showed expression under salt stress. Based on this result, CqR2R3-MYB s may regulate quinoa plant growth development and resistance to abiotic stresses. These findings provided comprehensive insights on role of CqR2R3-MYBs gene family members in quinoa and candidate MYB gene family members can be further studies on their role for abiotic stress tolerance in crop plants.
{"title":"Genome-wide identification, phylogeny and expression analysis of the <i>R2R3-MYB</i> gene family in quinoa (<i>Chenopodium quinoa</i>) under abiotic stress.","authors":"Pengcheng Ding, Peng Tang, Xiaofen Li, Adeela Haroon, Saima Nasreen, Hafeez Noor, Kotb A Attia, Asmaa M Abushady, Rongzhen Wang, Kaiyuan Cui, Xiangyun Wu, Min Sun, Zhiqiang Gao","doi":"10.1071/FP23261","DOIUrl":"10.1071/FP23261","url":null,"abstract":"<p><p>The MYB transcription factor (TF) are among the largest gene families of plants being responsible for several biological processes. The R2R3-MYB gene family are integral player regulating plant primary and secondary metabolism, growth and development, and responses to hormones and stresses. The phylogenetic analysis combined with gene structure analysis and motif determination resulted in division of R2R3-MYB gene family into 27 subgroups. Evidence generated from synteny analyses indicated that CqR2R3-MYBs gene family is featured by tandem and segmental duplication events. On the basis of RNA-Seq data, the expression patterns of different tissues under salt treatment were investigated resulting CqR2R3-MYB genes high expression both in roots and stem of quinoa (Chenopodium quinoa ) plants. More than half of CqR2R3-MYB genes showed expression under salt stress. Based on this result, CqR2R3-MYB s may regulate quinoa plant growth development and resistance to abiotic stresses. These findings provided comprehensive insights on role of CqR2R3-MYBs gene family members in quinoa and candidate MYB gene family members can be further studies on their role for abiotic stress tolerance in crop plants.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139989775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammadhadi Najafi, Mehdi Nasr-Esfahani, Jafar Vatandoost, Hamed Hassanzade-Khankahdani, Matin Jami Moeini
{"title":"<i>Corrigendum to</i>: Transcriptome-based analysis of candidate gene markers associated with resistance mechanism to <i>Phytophthora melonis</i> that causes root and crown rot in pumpkin.","authors":"Mohammadhadi Najafi, Mehdi Nasr-Esfahani, Jafar Vatandoost, Hamed Hassanzade-Khankahdani, Matin Jami Moeini","doi":"10.1071/FP23038_CO","DOIUrl":"https://doi.org/10.1071/FP23038_CO","url":null,"abstract":"","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140858151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tropospheric ozone (O3 ) is a significant abiotic stressor whose rising concentration negatively influences plant growth. Studies related to the differential response of Abelmoschus cytotypes to elevated O3 treatment are scarce and need further exploration to recognise the role of polyploidisation in stress tolerance. In this study, we analysed the changes in growth pattern, ultrastructure, physiology and foliar protein profile occurring under O3 stress in Abelmoschus moschatus (monoploid), Abelmoschus esculentus (diploid) and Abelmoschus caillei (triploid). Our findings showed that higher stomatal conductance in A. moschatus triggered higher O3 intake, causing damage to stomatal cells and photosynthetic pigments. Additionally, it caused a reduction in photosynthetic rates, leading to reduced plant growth, total biomass and economic yield. This O3 -induced toxicity was less in diploid and triploid cytotypes of Abelmoschus . Protein profiling by sodium dodecyl sulpate-polyacrylamide gel electrophoresis showed a significant decrease in the commonly found RuBisCO larger and smaller subunits. The decrease was more prominent in monoploid compared to diploid and triploid. This study provides crucial data for research that aim to enhance plant ability to withstand O3 induced oxidative stress. Our findings may help in developing a tolerant variety through plant breeding techniques, which will be economically more advantageous in reaching the objective of sustainable production at the high O3 levels projected under a climate change scenario.
{"title":"Growth, ultrastructural and physiological characteristics of <i>Abelmoschus</i> cytotypes under elevated ozone stress: a study on ploidy-specific responses.","authors":"Priyanka Singh, Naushad Ansari, Amit Kumar Mishra, Madhoolika Agrawal, Shashi Bhushan Agrawal","doi":"10.1071/FP23229","DOIUrl":"10.1071/FP23229","url":null,"abstract":"<p><p>Tropospheric ozone (O3 ) is a significant abiotic stressor whose rising concentration negatively influences plant growth. Studies related to the differential response of Abelmoschus cytotypes to elevated O3 treatment are scarce and need further exploration to recognise the role of polyploidisation in stress tolerance. In this study, we analysed the changes in growth pattern, ultrastructure, physiology and foliar protein profile occurring under O3 stress in Abelmoschus moschatus (monoploid), Abelmoschus esculentus (diploid) and Abelmoschus caillei (triploid). Our findings showed that higher stomatal conductance in A. moschatus triggered higher O3 intake, causing damage to stomatal cells and photosynthetic pigments. Additionally, it caused a reduction in photosynthetic rates, leading to reduced plant growth, total biomass and economic yield. This O3 -induced toxicity was less in diploid and triploid cytotypes of Abelmoschus . Protein profiling by sodium dodecyl sulpate-polyacrylamide gel electrophoresis showed a significant decrease in the commonly found RuBisCO larger and smaller subunits. The decrease was more prominent in monoploid compared to diploid and triploid. This study provides crucial data for research that aim to enhance plant ability to withstand O3 induced oxidative stress. Our findings may help in developing a tolerant variety through plant breeding techniques, which will be economically more advantageous in reaching the objective of sustainable production at the high O3 levels projected under a climate change scenario.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139680978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Le Yu, Jiankai Zhou, Junlong Lin, Mengwei Chen, Fang Liu, Xinlin Zheng, Liping Zhou, Ruozhong Wang, Langtao Xiao, Yonghai Liu
Phytohormones play a key role in regulating tiller number. Ascorbic acid (Asc)-phytohormone interaction plays a pivotal role in the regulation of senescence. We analysed the relationship between Asc and the enzyme concentrations and gene transcript abundances related to the signal perception of strigolactones (SLs), the contents of four phytohormones (abscisic acid, ABA; jasmonic acid, JA; indole acetic acid, IAA; cytokinin, CTK), the enzyme concentrations and gene transcript abundances related to the synthesis or transportation of these four phytohormones. Our results showed that Asc deficiency leads to the upregulation of enzyme concentrations, gene transcript abundances related to the SL signal perception, ABA synthesis and IAA transport. The altered level of Asc also leads to a change in the contents of ABA, JA, IAA and CTK. These findings support the conclusion that Asc or Asc/DHA play an important role in the signal perception and transduction of SLs, and Asc may affect the coordinated regulation of SL, IAA and CTK on rice (Oryza sativa ) tillering.
{"title":"Perception of strigolactones and the coordinated phytohormonal regulation on rice (<i>Oryza sativa</i>) tillering is affected by endogenous ascorbic acid.","authors":"Le Yu, Jiankai Zhou, Junlong Lin, Mengwei Chen, Fang Liu, Xinlin Zheng, Liping Zhou, Ruozhong Wang, Langtao Xiao, Yonghai Liu","doi":"10.1071/FP23148","DOIUrl":"10.1071/FP23148","url":null,"abstract":"<p><p>Phytohormones play a key role in regulating tiller number. Ascorbic acid (Asc)-phytohormone interaction plays a pivotal role in the regulation of senescence. We analysed the relationship between Asc and the enzyme concentrations and gene transcript abundances related to the signal perception of strigolactones (SLs), the contents of four phytohormones (abscisic acid, ABA; jasmonic acid, JA; indole acetic acid, IAA; cytokinin, CTK), the enzyme concentrations and gene transcript abundances related to the synthesis or transportation of these four phytohormones. Our results showed that Asc deficiency leads to the upregulation of enzyme concentrations, gene transcript abundances related to the SL signal perception, ABA synthesis and IAA transport. The altered level of Asc also leads to a change in the contents of ABA, JA, IAA and CTK. These findings support the conclusion that Asc or Asc/DHA play an important role in the signal perception and transduction of SLs, and Asc may affect the coordinated regulation of SL, IAA and CTK on rice (Oryza sativa ) tillering.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139702228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Ammar, Zulfiqar Ali, Muhammad Abu Bakar Saddique, Muhammad Habib-Ur-Rahman, Imtiaz Ali
Plants have certain adaptation mechanisms to combat temperature extremes and fluctuations. The heat shock protein (HSP90A) plays a crucial role in plant defence mechanisms under heat stress. In silico analysis of the eight TaHSP90A transcripts showed diverse structural patterns in terms of intron/exons, domains, motifs and cis elements in the promoter region in wheat. These regions contained cis elements related to hormones, biotic and abiotic stress and development. To validate these findings, two contrasting wheat genotypes E-01 (thermo-tolerant) and SHP-52 (thermo-sensitive) were used to evaluate the expression pattern of three transcripts TraesCS2A02G033700.1, TraesCS5B02G258900.3 and TraesCS5D02G268000.2 in five different tissues at five different temperature regimes. Expression of TraesCS2A02G033700.1 was upregulated (2-fold) in flag leaf tissue after 1 and 4h of heat treatment in E-01. In contrast, SHP-52 showed downregulated expression after 1h of heat treatment. Additionally, it was shown that under heat stress, the increased expression of TaHSP90A led to an increase in grain production. As the molecular mechanism of genes involved in heat tolerance at the reproductive stage is mostly unknown, these results provide new insights into the role of TaHSP90A transcripts in developing phenotypic plasticity in wheat to develop heat-tolerant cultivars under the current changing climate scenario.
{"title":"Upregulation of <i>TaHSP90A</i> transcripts enhances heat tolerance and increases grain yield in wheat under changing climate conditions.","authors":"Ali Ammar, Zulfiqar Ali, Muhammad Abu Bakar Saddique, Muhammad Habib-Ur-Rahman, Imtiaz Ali","doi":"10.1071/FP23275","DOIUrl":"10.1071/FP23275","url":null,"abstract":"<p><p>Plants have certain adaptation mechanisms to combat temperature extremes and fluctuations. The heat shock protein (HSP90A) plays a crucial role in plant defence mechanisms under heat stress. In silico analysis of the eight TaHSP90A transcripts showed diverse structural patterns in terms of intron/exons, domains, motifs and cis elements in the promoter region in wheat. These regions contained cis elements related to hormones, biotic and abiotic stress and development. To validate these findings, two contrasting wheat genotypes E-01 (thermo-tolerant) and SHP-52 (thermo-sensitive) were used to evaluate the expression pattern of three transcripts TraesCS2A02G033700.1, TraesCS5B02G258900.3 and TraesCS5D02G268000.2 in five different tissues at five different temperature regimes. Expression of TraesCS2A02G033700.1 was upregulated (2-fold) in flag leaf tissue after 1 and 4h of heat treatment in E-01. In contrast, SHP-52 showed downregulated expression after 1h of heat treatment. Additionally, it was shown that under heat stress, the increased expression of TaHSP90A led to an increase in grain production. As the molecular mechanism of genes involved in heat tolerance at the reproductive stage is mostly unknown, these results provide new insights into the role of TaHSP90A transcripts in developing phenotypic plasticity in wheat to develop heat-tolerant cultivars under the current changing climate scenario.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139702230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bahman Panahi, Mohammad Farhadian, Nahid Hosseinzadeh Gharajeh, Seyyed Abolghasem Mohammadi, Mohammad Amin Hejazi
Microalgae are photosynthetic organisms and a potential source of sustainable metabolite production. However, different stress conditions might affect the production of various metabolites. In this study, a meta-analysis of RNA-seq experiments in Dunaliella tertiolecta was evaluated to compare metabolite biosynthesis pathways in response to abiotic stress conditions such as high light, nitrogen deficiency and high salinity. Results showed downregulation of light reaction, photorespiration, tetrapyrrole and lipid-related pathways occurred under salt stress. Nitrogen deficiency mostly induced the microalgal responses of light reaction and photorespiration metabolism. Phosphoenol pyruvate carboxylase, phosphoglucose isomerase, bisphosphoglycerate mutase and glucose-6-phosphate-1-dehydrogenase (involved in central carbon metabolism) were commonly upregulated under salt, light and nitrogen stresses. Interestingly, the results indicated that the meta-genes (modules of genes strongly correlated) were located in a hub of stress-specific protein-protein interaction (PPI) network. Module enrichment of meta-genes PPI networks highlighted the cross-talk between photosynthesis, fatty acids, starch and sucrose metabolism under multiple stress conditions. Moreover, it was observed that the coordinated expression of the tetrapyrrole intermediated with meta-genes was involved in starch biosynthesis. Our results also showed that the pathways of vitamin B6 metabolism, methane metabolism, ribosome biogenesis and folate biosynthesis responded specifically to different stress factors. Since the results of this study revealed the main pathways underlying the abiotic stress, they might be applied in optimised metabolite production by the microalga Dunaliella in future studies. PRISMA check list was also included in the study.
微藻类是光合生物,是可持续代谢物生产的潜在来源。然而,不同的压力条件可能会影响各种代谢物的产生。本研究通过荟萃分析邓氏藻(Dunaliella tertiolecta)的RNA-seq实验,比较了代谢物生物合成途径对强光、缺氮和高盐度等非生物胁迫条件的响应。结果表明,在盐胁迫条件下,光反应、光呼吸、四吡咯和脂质相关途径发生下调。缺氮主要诱导微藻的光反应和光呼吸代谢反应。磷酸烯醇丙酮酸羧化酶、磷酸葡萄糖异构酶、双磷酸甘油酸突变酶和葡萄糖-6-磷酸-1-脱氢酶(参与中心碳代谢)在盐胁迫、光胁迫和氮胁迫下普遍上调。有趣的是,研究结果表明,元基因(高度相关的基因模块)位于胁迫特异性蛋白质-蛋白质相互作用(PPI)网络的中心。元基因 PPI 网络的模块富集突显了多种胁迫条件下光合作用、脂肪酸、淀粉和蔗糖代谢之间的交叉作用。此外,我们还观察到四吡咯中间体与元基因的协调表达参与了淀粉的生物合成。我们的研究结果还表明,维生素 B6 代谢、甲烷代谢、核糖体生物发生和叶酸生物合成的途径对不同胁迫因子有特异性响应。由于本研究的结果揭示了非生物胁迫的主要途径,因此在今后的研究中可能会应用于微藻杜纳利藻代谢物的优化生产。本研究还包括 PRISMA 检查清单。
{"title":"Meta-analysis of transcriptomic profiles in <i>Dunaliella tertiolecta</i> reveals molecular pathway responses to different abiotic stresses.","authors":"Bahman Panahi, Mohammad Farhadian, Nahid Hosseinzadeh Gharajeh, Seyyed Abolghasem Mohammadi, Mohammad Amin Hejazi","doi":"10.1071/FP23002","DOIUrl":"10.1071/FP23002","url":null,"abstract":"<p><p>Microalgae are photosynthetic organisms and a potential source of sustainable metabolite production. However, different stress conditions might affect the production of various metabolites. In this study, a meta-analysis of RNA-seq experiments in Dunaliella tertiolecta was evaluated to compare metabolite biosynthesis pathways in response to abiotic stress conditions such as high light, nitrogen deficiency and high salinity. Results showed downregulation of light reaction, photorespiration, tetrapyrrole and lipid-related pathways occurred under salt stress. Nitrogen deficiency mostly induced the microalgal responses of light reaction and photorespiration metabolism. Phosphoenol pyruvate carboxylase, phosphoglucose isomerase, bisphosphoglycerate mutase and glucose-6-phosphate-1-dehydrogenase (involved in central carbon metabolism) were commonly upregulated under salt, light and nitrogen stresses. Interestingly, the results indicated that the meta-genes (modules of genes strongly correlated) were located in a hub of stress-specific protein-protein interaction (PPI) network. Module enrichment of meta-genes PPI networks highlighted the cross-talk between photosynthesis, fatty acids, starch and sucrose metabolism under multiple stress conditions. Moreover, it was observed that the coordinated expression of the tetrapyrrole intermediated with meta-genes was involved in starch biosynthesis. Our results also showed that the pathways of vitamin B6 metabolism, methane metabolism, ribosome biogenesis and folate biosynthesis responded specifically to different stress factors. Since the results of this study revealed the main pathways underlying the abiotic stress, they might be applied in optimised metabolite production by the microalga Dunaliella in future studies. PRISMA check list was also included in the study.</p>","PeriodicalId":12483,"journal":{"name":"Functional Plant Biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139930693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}