Pub Date : 2023-05-12DOI: 10.4308/hjb.30.4.779-788
D. Priyandoko, W. Widowati, Ervi Afifah, Ika Adhani Sholihah, C. Wahyuni, C. Wijayanti, R. Rizal
Several studies have suggested that "cytokine storms" are significant causes of the severity of COVID-19. Controlling and inhibiting the cytokine storm in COVID-19 could prevent the spread of COVID-19 and saves patient lives. Soybean (Glycine max L.) is known to have various biological activities. This study aims to examine bioactive compounds in SSE and the effect of SSE on the ARDS rats model. A total of 25 Sprague Dawley Lipopolysaccharide-induced rats were used. Determination of serum IL-1β, IL-12, and lung TNF-α levels was performed by ELISA method. NF-κB and IFN-γ expression were determined by the qRTPCR method. IL-6 expressions were analyzed by immunohistochemistry assay. The bleeding, inflammation, and alveolus collapse score were analyzed using the HE staining method. The results showed that SSE could decrease the level of IL-1β, IL-12, TNF-α, IL-6, NF-kB, and IFN-γ and improve the bleeding, inflammation, and alveolus score in the lung. SSE could decrease the pro-inflammatory cytokines and improve lung condition in ARDS rats model.
几项研究表明，“细胞因子风暴”是导致新冠肺炎严重程度的重要原因。控制和抑制新冠肺炎中的细胞因子风暴可以防止新冠肺炎的传播并挽救患者的生命。大豆（Glycine max L.）具有多种生物学活性。本研究旨在检测SSE中的生物活性化合物以及SSE对ARDS大鼠模型的影响。使用总共25只Sprague-Dawley脂多糖诱导的大鼠。ELISA法测定血清IL-1β、IL-12和肺TNF-α水平。κB和IFN-γ的表达。免疫组化法检测IL-6的表达。采用HE染色法分析出血、炎症和肺泡塌陷评分。结果表明，SSE可降低IL-1β、IL-12、TNF-α、IL-6、NF-kB和IFN-γ的水平，改善肺出血、炎症和肺泡评分。SSE可降低ARDS大鼠模型的促炎细胞因子，改善肺功能。
{"title":"Soybean Seeds (Glycine max L.) Extract Against Cytokine Storm in ARDS Rat Model through Inhibiting Inflammation Marker","authors":"D. Priyandoko, W. Widowati, Ervi Afifah, Ika Adhani Sholihah, C. Wahyuni, C. Wijayanti, R. Rizal","doi":"10.4308/hjb.30.4.779-788","DOIUrl":"https://doi.org/10.4308/hjb.30.4.779-788","url":null,"abstract":"Several studies have suggested that \"cytokine storms\" are significant causes of the severity of COVID-19. Controlling and inhibiting the cytokine storm in COVID-19 could prevent the spread of COVID-19 and saves patient lives. Soybean (Glycine max L.) is known to have various biological activities. This study aims to examine bioactive compounds in SSE and the effect of SSE on the ARDS rats model. A total of 25 Sprague Dawley Lipopolysaccharide-induced rats were used. Determination of serum IL-1β, IL-12, and lung TNF-α levels was performed by ELISA method. NF-κB and IFN-γ expression were determined by the qRTPCR method. IL-6 expressions were analyzed by immunohistochemistry assay. The bleeding, inflammation, and alveolus collapse score were analyzed using the HE staining method. The results showed that SSE could decrease the level of IL-1β, IL-12, TNF-α, IL-6, NF-kB, and IFN-γ and improve the bleeding, inflammation, and alveolus score in the lung. SSE could decrease the pro-inflammatory cytokines and improve lung condition in ARDS rats model.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43279407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-11DOI: 10.4308/hjb.30.4.770-778
Alfi Hudatul Karomah, M. Rafi, D. Septaningsih, A. Ilmiawati, Utami Dyah Safitri, N. Aminah, M. Insanu, A. Rohman
Sida rhombifolia, also known as sidaguri in Indonesia, is a medicinal plant commonly used as a herbal medicine because of its metabolite and biological activities. One of the several factors that affect plant metabolite composition and concentration is the use of plant parts. In this study, the experiment aimed to identify the metabolite profile in the leaves and stem extracts of S. rhombifolia using UHPLC-Q-Orbitrap HRMS-based untargeted metabolomics. The samples were distinguished by principal component analysis (PCA). Extraction of metabolites was conducted by sonication for approximately 30 min with 70% ethanol as the extraction solvent; 28 metabolites were identified. Seven metabolites were identified only in the leaves, three were identified only in the stems, and 18 other metabolites were identified in both the leaves and stems. These metabolites were categorized as flavonoids, triterpenoids, alkaloids, coumarins, phenolic aldehydes, phenolic acids, ecdysteroids, fatty acids, and monoterpene lactones. Based on the classification results, PCA grouped the leaves and stem extracts of S. rhombifolia using the peak area variables of the identified metabolites.
{"title":"UHPLC-Q-Orbitrap HRMS-based Untargeted Metabolomics of Sida rhombifolia Leaves and Stem Extracts","authors":"Alfi Hudatul Karomah, M. Rafi, D. Septaningsih, A. Ilmiawati, Utami Dyah Safitri, N. Aminah, M. Insanu, A. Rohman","doi":"10.4308/hjb.30.4.770-778","DOIUrl":"https://doi.org/10.4308/hjb.30.4.770-778","url":null,"abstract":"Sida rhombifolia, also known as sidaguri in Indonesia, is a medicinal plant commonly used as a herbal medicine because of its metabolite and biological activities. One of the several factors that affect plant metabolite composition and concentration is the use of plant parts. In this study, the experiment aimed to identify the metabolite profile in the leaves and stem extracts of S. rhombifolia using UHPLC-Q-Orbitrap HRMS-based untargeted metabolomics. The samples were distinguished by principal component analysis (PCA). Extraction of metabolites was conducted by sonication for approximately 30 min with 70% ethanol as the extraction solvent; 28 metabolites were identified. Seven metabolites were identified only in the leaves, three were identified only in the stems, and 18 other metabolites were identified in both the leaves and stems. These metabolites were categorized as flavonoids, triterpenoids, alkaloids, coumarins, phenolic aldehydes, phenolic acids, ecdysteroids, fatty acids, and monoterpene lactones. Based on the classification results, PCA grouped the leaves and stem extracts of S. rhombifolia using the peak area variables of the identified metabolites.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47623944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-10DOI: 10.4308/hjb.30.4.757-769
A. Gafur, M. Hodda
Males and swollen mature females of Achlysiella trilineata and Achlysiella magniglans were discovered for the first time in Australia. These life stages confirmed that both species show well-marked sexual dimorphism in the anterior region of the body, with the males having reduced cephalic frameworks, stylets, and oesophagi. Males of A. magniglans displayed an extreme reduction in the style such that only the cone could be seen, and the rest of the esophagus was barely visible. In both species, the swollen females had postmedian vulvae, and the anterior branch of the ovary was longer than the posterior branch. In A. magniglans, the disparity in length was more pronounced, and the vulva was more posterior. The finding of swollen females confirmed the classification of both species in Achlysiella rather than Radopholus. Vermiform females of both species have large dorsal oesophageal glands and short ovaries. Justifiably, other species of Radopholus with these latter features (R. brevicaudatus, R. capitatus, and R. vacuus) should also be moved to Achlysiella.
{"title":"First Records of Male and Swollen Females of Two Species of Achlysiella (Nematoda: Pratylenchidae)","authors":"A. Gafur, M. Hodda","doi":"10.4308/hjb.30.4.757-769","DOIUrl":"https://doi.org/10.4308/hjb.30.4.757-769","url":null,"abstract":"Males and swollen mature females of Achlysiella trilineata and Achlysiella magniglans were discovered for the first time in Australia. These life stages confirmed that both species show well-marked sexual dimorphism in the anterior region of the body, with the males having reduced cephalic frameworks, stylets, and oesophagi. Males of A. magniglans displayed an extreme reduction in the style such that only the cone could be seen, and the rest of the esophagus was barely visible. In both species, the swollen females had postmedian vulvae, and the anterior branch of the ovary was longer than the posterior branch. In A. magniglans, the disparity in length was more pronounced, and the vulva was more posterior. The finding of swollen females confirmed the classification of both species in Achlysiella rather than Radopholus. Vermiform females of both species have large dorsal oesophageal glands and short ovaries. Justifiably, other species of Radopholus with these latter features (R. brevicaudatus, R. capitatus, and R. vacuus) should also be moved to Achlysiella.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46941704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-02DOI: 10.4308/hjb.30.4.743-756
N. Fadli, A. Damora, Z. Muchlisin, I. Dewiyanti, M. Ramadhaniaty, N. M. Razi, J. Jamaluddin, E. Macusi, M. N. Siti-Azizah
Understanding the patterns of genetic diversity of species and precise stock identification are important in fisheries conservation management. However, studies on genetic diversity, connectivity, population structure, and gene flow of groupers within the Indo-Malaya Archipelago (IMA) waters are limited. The objective of the present study was to examine the phylogeographic patterns of the family Epinephelidae in IMA waters based on a parallel study of five selected species utilizing the mitochondrial COI. The grouper species were: areolate grouper (Epinephelus areolatus), blacktip grouper (Epinephelus fasciatus), six-bar grouper (Epinephelus sexfasciatus), blue-lined hind (Cephalopholis formosa), and white-edged lyretail (Variola albimarginata). Specimens were obtained from fish landing sites and fish markets from 23 locations throughout the IMA waters. This study showed genetic structuring for two species (Epinephelus areolatus and Variola albimarginata) but genetic homogeneity for the other three investigated species across IMA. Various geological and demographic histories, local and regional oceanographic features, and biological characteristics are hypothesized to shape the present genetic pattern of each species across the IMA waters. The establishment of effective international cooperation is encouraged to manage grouper species stocks in this region.
{"title":"Phylogeographic Insights of Five Co-Habiting Grouper Species in The Indo-Malaya Archipelago","authors":"N. Fadli, A. Damora, Z. Muchlisin, I. Dewiyanti, M. Ramadhaniaty, N. M. Razi, J. Jamaluddin, E. Macusi, M. N. Siti-Azizah","doi":"10.4308/hjb.30.4.743-756","DOIUrl":"https://doi.org/10.4308/hjb.30.4.743-756","url":null,"abstract":"Understanding the patterns of genetic diversity of species and precise stock identification are important in fisheries conservation management. However, studies on genetic diversity, connectivity, population structure, and gene flow of groupers within the Indo-Malaya Archipelago (IMA) waters are limited. The objective of the present study was to examine the phylogeographic patterns of the family Epinephelidae in IMA waters based on a parallel study of five selected species utilizing the mitochondrial COI. The grouper species were: areolate grouper (Epinephelus areolatus), blacktip grouper (Epinephelus fasciatus), six-bar grouper (Epinephelus sexfasciatus), blue-lined hind (Cephalopholis formosa), and white-edged lyretail (Variola albimarginata). Specimens were obtained from fish landing sites and fish markets from 23 locations throughout the IMA waters. This study showed genetic structuring for two species (Epinephelus areolatus and Variola albimarginata) but genetic homogeneity for the other three investigated species across IMA. Various geological and demographic histories, local and regional oceanographic features, and biological characteristics are hypothesized to shape the present genetic pattern of each species across the IMA waters. The establishment of effective international cooperation is encouraged to manage grouper species stocks in this region.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43572762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.4308/hjb.30.4.734-742
Nadila Fany Ashafani, R. Astuti, S. Listiyowati, Irmanida Batubara
Metabolites from clove (Syzygium aromaticum L.) have been extensively studied for various pharmacological uses, including quercetin. Endophytic bacteria isolated from plant tissues can produce similar secondary metabolites as their host plants. However, little is known about the antioxidant activity of the endophytic bacterial extract from clove leaves. Amongst the potential bacterial groups, the genera of Bacillus have been widely studied as a source of antioxidants. Thus, this study, we examined the potential antioxidant properties of Bacillus sp., isolated from clove leaves. We have successfully isolated ten endophytic bacteria, of which only one isolate (P1U2) was found non-pathogenic based on a hemolytic assay. Based on the 16S rRNA sequence, P1U2 shared the highest similarity to Fictibacillus phosphorivorans. The extract of extracellular metabolites of P1U2 contains quercetin compounds, based on LC-MS analysis. The results of the antioxidant activity test using the DPPH assay showed that the P1U2 extract had an IC50 value of 161 mg/ml. The supplementation of the extract on culture media reduced the ability of S. cerevisiae to decolorize mutagen malachite green and increased cell viability. Furthermore, the addition of 161 ppm extract was able to counteract UV mutagens effects of UV exposure and toxicity of malachite green, significantly increasing the growth of S. cerevisiae. Suggesting the bioactivity of the extract in combating mutagens. To the best of our knowledge, this is the first study to report a quercetin-rich extract derived from endophytic bacteria, F. phosphorivorans. Thus, further study is required to optimize the synthesis of quercetin from isolate P1U2.
{"title":"Quercetin-Containing Extract from Clove Syzygium aromaticum L. Endophytic Bacteria, Fictibacillus phosphorivorans P1U2, Exhibits Antimutagenic Activity in Yeast Saccharomyces cerevisiae","authors":"Nadila Fany Ashafani, R. Astuti, S. Listiyowati, Irmanida Batubara","doi":"10.4308/hjb.30.4.734-742","DOIUrl":"https://doi.org/10.4308/hjb.30.4.734-742","url":null,"abstract":"Metabolites from clove (Syzygium aromaticum L.) have been extensively studied for various pharmacological uses, including quercetin. Endophytic bacteria isolated from plant tissues can produce similar secondary metabolites as their host plants. However, little is known about the antioxidant activity of the endophytic bacterial extract from clove leaves. Amongst the potential bacterial groups, the genera of Bacillus have been widely studied as a source of antioxidants. Thus, this study, we examined the potential antioxidant properties of Bacillus sp., isolated from clove leaves. We have successfully isolated ten endophytic bacteria, of which only one isolate (P1U2) was found non-pathogenic based on a hemolytic assay. Based on the 16S rRNA sequence, P1U2 shared the highest similarity to Fictibacillus phosphorivorans. The extract of extracellular metabolites of P1U2 contains quercetin compounds, based on LC-MS analysis. The results of the antioxidant activity test using the DPPH assay showed that the P1U2 extract had an IC50 value of 161 mg/ml. The supplementation of the extract on culture media reduced the ability of S. cerevisiae to decolorize mutagen malachite green and increased cell viability. Furthermore, the addition of 161 ppm extract was able to counteract UV mutagens effects of UV exposure and toxicity of malachite green, significantly increasing the growth of S. cerevisiae. Suggesting the bioactivity of the extract in combating mutagens. To the best of our knowledge, this is the first study to report a quercetin-rich extract derived from endophytic bacteria, F. phosphorivorans. Thus, further study is required to optimize the synthesis of quercetin from isolate P1U2.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41299776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.4308/hjb.30.4.716-724
L. M. Jaelani, Benedict, Diah Ardiani, M. P. Tambunan, M. Indrawan, Andri A. Wibowo
As an endangered species, Anoa (Bubalus depressicornis and Mountain Bubalus quarlesi) inhabiting the Sulawesi island requires proper conservation both in and out of their native habitat. The study of anoa habitat is mainly conducted through field studies based on firsthand observations of anoa appearance, footprints, or excrement or through social surveys from residents who saw it directly. The studies are carried out specifically in a particular area with a relatively narrow. However, in practice, this method has limitations, such as the research location determined based on the possibility of anoa, limited research area, and inefficient use of resources. Therefore, this study aimed to model the potential habitat of anoa in the whole of Sulawesi island. This study was based on physical and environmental independent variables such as DEM, surface slope, LST, NDVI, and access to inland water, as well as in-situ species distribution retrieved from scientific papers and reports. This study discovered the likely anoa distribution on Sulawesi island, both inside and outside of its native habitat. LST is the most important independent variable in determining habitat suitability, accounting for 80% of the total, followed by water (15.3%), NDVI (2.9%), DEM (1.6%), and slope (0.3%).
{"title":"An Extensive Coverage Anoa Distribution Modelling in Sulawesi Using Maximum Entropy","authors":"L. M. Jaelani, Benedict, Diah Ardiani, M. P. Tambunan, M. Indrawan, Andri A. Wibowo","doi":"10.4308/hjb.30.4.716-724","DOIUrl":"https://doi.org/10.4308/hjb.30.4.716-724","url":null,"abstract":"As an endangered species, Anoa (Bubalus depressicornis and Mountain Bubalus quarlesi) inhabiting the Sulawesi island requires proper conservation both in and out of their native habitat. The study of anoa habitat is mainly conducted through field studies based on firsthand observations of anoa appearance, footprints, or excrement or through social surveys from residents who saw it directly. The studies are carried out specifically in a particular area with a relatively narrow. However, in practice, this method has limitations, such as the research location determined based on the possibility of anoa, limited research area, and inefficient use of resources. Therefore, this study aimed to model the potential habitat of anoa in the whole of Sulawesi island. This study was based on physical and environmental independent variables such as DEM, surface slope, LST, NDVI, and access to inland water, as well as in-situ species distribution retrieved from scientific papers and reports. This study discovered the likely anoa distribution on Sulawesi island, both inside and outside of its native habitat. LST is the most important independent variable in determining habitat suitability, accounting for 80% of the total, followed by water (15.3%), NDVI (2.9%), DEM (1.6%), and slope (0.3%).","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45497658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-17DOI: 10.4308/hjb.30.4.725-733
F. Ekawasti, Eko Setyo Purwanto, Farlin Nepho, D. A. Kurniawati, D. Subekti, R. Damayanti, S. Sa’diah, U. Cahyaningsih
Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of the locus has been carried out to determine the different genotypes of T. gondii (strain types I, II, and III) that are associated with human toxoplasmosis. The several genotypes of T. gondii (strain types I, II, and III) that are linked to human toxoplasmosis have been identified through genetic study of the locus. In this investigation, PCR-RFLP was found to be a useful, and simple method genotypic characterization. The objective of this study was to genotyping characterize T. gondii RH and BEV strains isolates by PCR-RFLP using several restriction enzymes. T. gondii tachyzoite DNA was extracted and amplified by PCR using dense granule genetic markers (GRA1 and GRA7) designed with Primer3plus. The amplification were digested using the restriction enzymes. The PCR-RFLP amplified dense granule products was used to classify strains into two genotypes of T. gondii (virulent and avirulent). The results demonstrated that the RFLP patterns of the GRA1 and GRA7 gene area digested by DdeI, MvaI, HinfI, RsaI, and Sau96I enzymes can be used to identify virulent or avirulent strains of T. gondii. Toxoplasma gondii RH and BEV strain produced different digestion product which can be used to distinguished the strains.
{"title":"The Patterns of Restriction Fragment of Several Enzymes to Distinguish Toxoplasma gondii Isolates Virulent and Avirulent Strains using GRA1 and GRA7 Genetic Marker","authors":"F. Ekawasti, Eko Setyo Purwanto, Farlin Nepho, D. A. Kurniawati, D. Subekti, R. Damayanti, S. Sa’diah, U. Cahyaningsih","doi":"10.4308/hjb.30.4.725-733","DOIUrl":"https://doi.org/10.4308/hjb.30.4.725-733","url":null,"abstract":"Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of the locus has been carried out to determine the different genotypes of T. gondii (strain types I, II, and III) that are associated with human toxoplasmosis. The several genotypes of T. gondii (strain types I, II, and III) that are linked to human toxoplasmosis have been identified through genetic study of the locus. In this investigation, PCR-RFLP was found to be a useful, and simple method genotypic characterization. The objective of this study was to genotyping characterize T. gondii RH and BEV strains isolates by PCR-RFLP using several restriction enzymes. T. gondii tachyzoite DNA was extracted and amplified by PCR using dense granule genetic markers (GRA1 and GRA7) designed with Primer3plus. The amplification were digested using the restriction enzymes. The PCR-RFLP amplified dense granule products was used to classify strains into two genotypes of T. gondii (virulent and avirulent). The results demonstrated that the RFLP patterns of the GRA1 and GRA7 gene area digested by DdeI, MvaI, HinfI, RsaI, and Sau96I enzymes can be used to identify virulent or avirulent strains of T. gondii. Toxoplasma gondii RH and BEV strain produced different digestion product which can be used to distinguished the strains.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46252736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-12DOI: 10.4308/hjb.30.4.701-710
Akhmad Abror As Sidiqi, C. M. Airin, Sarmin Sarmin, P. Astuti
Aromatase blocker effectively promotes testosterone, which improves muscle performance and protein synthesis. Considerable muscle strength in Bangkok rooster is the outcome of testosterone activity. Clamshell contains aromatase blocker substances, while fishbone possesses a high amount of protein. This research aimed to evaluate the clamshell and fishbone combination potency towards testosterone levels and muscle performance in Bangkok roosters. This study employed 18 Bangkok roosters under 35 days of treatment. The rooster groups consisted of control (P0, n = 6); clamshell-fishbone (P1, n = 6): clamshell (6.6 gram/day) + fishbone (3.3 gram/day); fishbone (P2, n = 6): fishbone (3.3 gram/day). The blood was collected once every seven days. Enzyme immunoassay showed the highest serum and muscle testosterone levels in P1 (p<0.05). Immunohistochemistry presented the most and broadest myofiber and the highest proliferation activity in P1 (p<0.05). The texture analysis showed the topmost springiness index in P1 (p<0.05). Aromatase blockers in clamshell and protein in fishbone improve testosterone and muscle performance in Bangkok roosters.
芳香酶阻滞剂有效促进睾酮，提高肌肉性能和蛋白质合成。曼谷公鸡相当大的肌肉力量是睾酮活动的结果。蛤壳含有芳香酶阻滞剂，而鱼骨含有大量的蛋白质。本研究旨在评价蛤壳和鱼骨组合对曼谷公鸡睾酮水平和肌肉性能的影响。本研究选用18只治疗35 d的曼谷公鸡。鸡组为对照组(P0, n = 6);翻贝-鱼骨(P1, n = 6):翻贝(6.6克/天)+鱼骨(3.3克/天);鱼骨(P2, n = 6):鱼骨(3.3克/天)。每七天采集一次血。酶免疫分析显示P1组血清和肌肉睾酮水平最高(p<0.05)。免疫组化显示P1的肌纤维最多、最宽，增殖活性最高(p<0.05)。织构分析显示，P1弹性指数最高(p<0.05)。蛤壳中的芳香酶阻滞剂和鱼骨中的蛋白质可提高曼谷公鸡的睾酮水平和肌肉性能。
{"title":"A Combination of Anadara nodifera Shell and Milkfish Thorns Powder Effectively Promote Springiness Index, Serum Testosterone, and Breast Muscle Testosterone in Bangkok Rooster","authors":"Akhmad Abror As Sidiqi, C. M. Airin, Sarmin Sarmin, P. Astuti","doi":"10.4308/hjb.30.4.701-710","DOIUrl":"https://doi.org/10.4308/hjb.30.4.701-710","url":null,"abstract":"Aromatase blocker effectively promotes testosterone, which improves muscle performance and protein synthesis. Considerable muscle strength in Bangkok rooster is the outcome of testosterone activity. Clamshell contains aromatase blocker substances, while fishbone possesses a high amount of protein. This research aimed to evaluate the clamshell and fishbone combination potency towards testosterone levels and muscle performance in Bangkok roosters. This study employed 18 Bangkok roosters under 35 days of treatment. The rooster groups consisted of control (P0, n = 6); clamshell-fishbone (P1, n = 6): clamshell (6.6 gram/day) + fishbone (3.3 gram/day); fishbone (P2, n = 6): fishbone (3.3 gram/day). The blood was collected once every seven days. Enzyme immunoassay showed the highest serum and muscle testosterone levels in P1 (p<0.05). Immunohistochemistry presented the most and broadest myofiber and the highest proliferation activity in P1 (p<0.05). The texture analysis showed the topmost springiness index in P1 (p<0.05). Aromatase blockers in clamshell and protein in fishbone improve testosterone and muscle performance in Bangkok roosters.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43390494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-12DOI: 10.4308/hjb.30.4.711-715
D. H. Quyet, P. T. Dung
Fish bodies respond to changes in environmental conditions in various ways and degrees. Seven fish species were collected from three study locations, including two nearshore sites (Ha Tinh province and Nha Trang city, Khanh Hoa province) and one offshore site (Toc Tan island, Khanh Hoa province) to evaluate alterations in erythrocyte morphology under certain environmental conditions. Heavy metal concentrations at all study sites were analyzed and met the acceptable technical criteria for each site. Fish blood samples were taken from the tail veins, anticoagulated with heparin, and stained with Giemsa stain. The blood cell morphological study was performed using an optical microscope. The results revealed two types of morphological abnormalities of fish erythrocytes, including nuclear deformation and nuclear-matter distribution.
{"title":"Morphological Alterations of Fish Erythrocytes as Their Response to Environmental Conditions","authors":"D. H. Quyet, P. T. Dung","doi":"10.4308/hjb.30.4.711-715","DOIUrl":"https://doi.org/10.4308/hjb.30.4.711-715","url":null,"abstract":"Fish bodies respond to changes in environmental conditions in various ways and degrees. Seven fish species were collected from three study locations, including two nearshore sites (Ha Tinh province and Nha Trang city, Khanh Hoa province) and one offshore site (Toc Tan island, Khanh Hoa province) to evaluate alterations in erythrocyte morphology under certain environmental conditions. Heavy metal concentrations at all study sites were analyzed and met the acceptable technical criteria for each site. Fish blood samples were taken from the tail veins, anticoagulated with heparin, and stained with Giemsa stain. The blood cell morphological study was performed using an optical microscope. The results revealed two types of morphological abnormalities of fish erythrocytes, including nuclear deformation and nuclear-matter distribution.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42414787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-04-11DOI: 10.4308/hjb.30.4.692-700
Yuriko Asahiro, A. Sunkar, Jarwadi Budi Hernowo
Stress can be defined as psychological and physical tension caused by physical, emotional, social, economic, and occupational, events, or experiences that are difficult to manage or endure. Bird sounds can be a potential solution to reduce stress levels. This study aimed to analyze the effect of bird sounds as a stress restorative on Bogor Botanical Garden visitors. A survey of 100 visitors of Bogor Botanical Garden was conducted based on the Perceived Stress Scale and Perceived Restorativeness Soundscape Scale. Data on respondent characteristics were analyzed using descriptive analysis, the relationship between stress levels and perceived stress restorative effect of bird sound was analyzed using Pearson correlation analysis. The analysis showed that 78% of respondents had moderate to high perceived stress levels and 98% of respondents rated the degree of restoration from bird sounds to be moderate to high. The correlation analysis revealed a negative relationship between the stress level and bird sounds' perceived restorative effect. The older the respondents and the more frequently they visited Bogor Botanical Garden, the higher the perceived stress restorative effect from bird sound.
{"title":"Bird Sounds Psychological Restorative Effect on The Visitors of Bogor Botanical Garden","authors":"Yuriko Asahiro, A. Sunkar, Jarwadi Budi Hernowo","doi":"10.4308/hjb.30.4.692-700","DOIUrl":"https://doi.org/10.4308/hjb.30.4.692-700","url":null,"abstract":"Stress can be defined as psychological and physical tension caused by physical, emotional, social, economic, and occupational, events, or experiences that are difficult to manage or endure. Bird sounds can be a potential solution to reduce stress levels. This study aimed to analyze the effect of bird sounds as a stress restorative on Bogor Botanical Garden visitors. A survey of 100 visitors of Bogor Botanical Garden was conducted based on the Perceived Stress Scale and Perceived Restorativeness Soundscape Scale. Data on respondent characteristics were analyzed using descriptive analysis, the relationship between stress levels and perceived stress restorative effect of bird sound was analyzed using Pearson correlation analysis. The analysis showed that 78% of respondents had moderate to high perceived stress levels and 98% of respondents rated the degree of restoration from bird sounds to be moderate to high. The correlation analysis revealed a negative relationship between the stress level and bird sounds' perceived restorative effect. The older the respondents and the more frequently they visited Bogor Botanical Garden, the higher the perceived stress restorative effect from bird sound.","PeriodicalId":12927,"journal":{"name":"HAYATI Journal of Biosciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44259165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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