Pub Date : 2023-05-30DOI: 10.5604/01.3001.0053.6135
R. Ramadhan, E. Rosamah, Irawan Wijaya Kusuma, H. Kuspradini, Yhiya Amen, Yong-Ung Kim, Kuniyoshi Shimizu, Enos Tangke Arung
Propolis is a bee product traditionally used for therapy in several diseases. The therapeutic activities of propolis, such as antioxidant, antifungal, antibacterial, and anti-inflammatory, are commonly known. Propolis from the stingless bee (Wallacetrigonainsica) showed promising bioactivity essential for the criteria.
{"title":"Cytotoxicity effect of prenylated flavonoids from Stingless Bee (Wallacetrigona insica) propolis in some cancer cell lines","authors":"R. Ramadhan, E. Rosamah, Irawan Wijaya Kusuma, H. Kuspradini, Yhiya Amen, Yong-Ung Kim, Kuniyoshi Shimizu, Enos Tangke Arung","doi":"10.5604/01.3001.0053.6135","DOIUrl":"https://doi.org/10.5604/01.3001.0053.6135","url":null,"abstract":"Propolis is a bee product traditionally used for therapy in several diseases. The therapeutic activities of propolis, such as antioxidant, antifungal, antibacterial, and anti-inflammatory, are commonly known. Propolis from the stingless bee (Wallacetrigonainsica) showed promising bioactivity essential for the criteria.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45255618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.5604/01.3001.0053.6132
P. Das, D. -, A. Nayak, P. -, P. K, N. K., A. Kiruba
Objective: One of the declining medicinal plant species is Coptis teeta Wall, found solely in eastern parts ofIndia and China. The major chemical constituents present in the plant are berberine, pentadecanoic acid,copisine, palmatine, columbamine, epiberberineand jateorrhizine. The aim of present study is screening thephytoconstitutes in methanolic extract of roots by GC-MS analysis and isolating the pentadecanoic acid,aligned with DNA gyrase subunit B protein target involved in bacterial infection by carrying out moleculardocking.Methods: Each concentrate of Coptis teeta was evaluated for antibacterial action by plate disseminationtechnique against gram-positive and gram-negative microorganisms.GC-MS was performed for theidentification of various phytochemical constituents present in methanolic extracts of Coptis teeta.Out ofvarious chemical constituents,pentadecanoic acid was isolated and characterised by IR, 1HNMR and MASSspectroscopy.The isolated pentadecanoic acid was screened against bacterial strains such as Staphylococcusaureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia colihave shown good antibacterialactivity.Results: Column chromatography was performed for separation andGC-MS showed the presence ofpentadecanoic acid by a significant spot on methanolic extract, characterized by IR, NMR and MASS. Themethanolic extract and isolated compoundhas shown good antibacterial activity.Protein-ligand dockingwas achieved between the protein PDBID: 1KZM and pentadecanoic acid which showed a good affinityindicating its potent inhibiting action.Conclusion:In binding studies, ligand showed an interaction with amino acids residue His 170, Ala 140 andSer 246 of 1KZM which indicated the inhibition of bacterial growth.
{"title":"Antibacterial activity and molecular docking study of Coptis teeta","authors":"P. Das, D. -, A. Nayak, P. -, P. K, N. K., A. Kiruba","doi":"10.5604/01.3001.0053.6132","DOIUrl":"https://doi.org/10.5604/01.3001.0053.6132","url":null,"abstract":"Objective: One of the declining medicinal plant species is Coptis teeta Wall, found solely in eastern parts ofIndia and China. The major chemical constituents present in the plant are berberine, pentadecanoic acid,copisine, palmatine, columbamine, epiberberineand jateorrhizine. The aim of present study is screening thephytoconstitutes in methanolic extract of roots by GC-MS analysis and isolating the pentadecanoic acid,aligned with DNA gyrase subunit B protein target involved in bacterial infection by carrying out moleculardocking.Methods: Each concentrate of Coptis teeta was evaluated for antibacterial action by plate disseminationtechnique against gram-positive and gram-negative microorganisms.GC-MS was performed for theidentification of various phytochemical constituents present in methanolic extracts of Coptis teeta.Out ofvarious chemical constituents,pentadecanoic acid was isolated and characterised by IR, 1HNMR and MASSspectroscopy.The isolated pentadecanoic acid was screened against bacterial strains such as Staphylococcusaureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia colihave shown good antibacterialactivity.Results: Column chromatography was performed for separation andGC-MS showed the presence ofpentadecanoic acid by a significant spot on methanolic extract, characterized by IR, NMR and MASS. Themethanolic extract and isolated compoundhas shown good antibacterial activity.Protein-ligand dockingwas achieved between the protein PDBID: 1KZM and pentadecanoic acid which showed a good affinityindicating its potent inhibiting action.Conclusion:In binding studies, ligand showed an interaction with amino acids residue His 170, Ala 140 andSer 246 of 1KZM which indicated the inhibition of bacterial growth.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49414764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.5604/01.3001.0053.7210
K. Ashokkumar, A. Pandian, M. Baskar, M. Karthik, M. Murugan, Ruchi Badoni Semwal, Deepak Kumar Semwal
Ashwagandha (Withania somnifera L. Dunal) roots have been used in Ayurveda for fever, asthma, arthritis,rheumatism, inflammation, tuberculosis, mental diseases, and male sexual disorders. Phytochemical studiesrevealed that the plant mainly contains alkaloids (withanine, sominiferine, tropine, isopelletierine andanaferine), and steroidal lactones (withanolides A-Y, withaferine A, withasominiferols A-C, withanone and sitoindosides) as bioactive metabolites. These constituents and extracts have several pharmacologicalactivities, including antioxidant, antitumour, antimicrobial, antivenom, and anti-parkinsonian activities.The present review presents updated information on ashwagandha based on its phytochemistry andpharmacology. The plant and many of its secondary metabolites have been found to be effective in thetreatment of many human ailments. However, further investigations are still needed to understand the exactmechanism of their actions.
在阿育吠陀中，印度蒺藜(Withania somnifera L. Dunal)的根被用来治疗发烧、哮喘、关节炎、风湿病、炎症、肺结核、精神疾病和男性性功能障碍。植物化学研究表明，该植物主要含有生物碱(茶氨酸、半胱氨酸、托品碱、异芥子碱和茶茶碱)和甾体内酯(茶茶素A- y、茶茶素A、茶茶素A- c、茶茶酮和谷甾醇苷)作为生物活性代谢产物。这些成分和提取物具有多种药理活性，包括抗氧化、抗肿瘤、抗菌、抗蛇毒血清和抗帕金森病活性。本文从植物化学和药理的角度综述了印度葫芦的最新研究进展。人们发现这种植物及其许多次生代谢产物对治疗许多人类疾病有效。然而，仍需要进一步的研究来了解其作用的确切机制。
{"title":"Withania somnifera a journey from traditionalrejuvenator to anti-SARS-CoV-2 remedy","authors":"K. Ashokkumar, A. Pandian, M. Baskar, M. Karthik, M. Murugan, Ruchi Badoni Semwal, Deepak Kumar Semwal","doi":"10.5604/01.3001.0053.7210","DOIUrl":"https://doi.org/10.5604/01.3001.0053.7210","url":null,"abstract":"Ashwagandha (Withania somnifera L. Dunal) roots have been used in Ayurveda for fever, asthma, arthritis,rheumatism, inflammation, tuberculosis, mental diseases, and male sexual disorders. Phytochemical studiesrevealed that the plant mainly contains alkaloids (withanine, sominiferine, tropine, isopelletierine andanaferine), and steroidal lactones (withanolides A-Y, withaferine A, withasominiferols A-C, withanone and sitoindosides) as bioactive metabolites. These constituents and extracts have several pharmacologicalactivities, including antioxidant, antitumour, antimicrobial, antivenom, and anti-parkinsonian activities.The present review presents updated information on ashwagandha based on its phytochemistry andpharmacology. The plant and many of its secondary metabolites have been found to be effective in thetreatment of many human ailments. However, further investigations are still needed to understand the exactmechanism of their actions.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71287500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sumia Urai̇nab, S. Kamal, Sevinj Musayeva Vagif, Arifa Mahreen, Saba Zafar
Summary Introduction: Feronia limonia (synonyms: Feronia elephantum, Hesperethusa crenulata, Limonia acidissima Linn, Schinus limonia) is a member of Rutaceae family. F. limonia is an aesthetically pleasing plant with various medicinal and nutraceutical properties. Objective: The primary objective of this research was to determine the flavonoid from F. limonia. Methods: Different solvents have been soxhleted to the roots of F. limonia powder. Column chromatography was performed using silica gel, a yellow amorphous solid was obtained from ethyl acetate extract after elution with hexane: ethyl acetate (4:8). Results: We have successfully isolated a unique geranyl flavanone from the roots of F. limonia. The structure was verified as 8-(2′′,3′′-epoxy-3′′-methyl butyl)-4′-prenyloxy flavanone-7-O-6′′′′-acetyl β-D-glucopyranoside. Conclusion: According to the findings based upon spectroscopic analysis, a hitherto unknown flavanone was isolated in the F. limonia.
简介:柠檬铁(Feronia limonia，别名:Feronia elephantum, Hesperethusa crenulata, limonia acidissima Linn, Schinus limonia)是芸香科植物。柠檬草是一种美观的植物，具有多种药用和营养价值。目的:测定枳实中黄酮类化合物的含量。方法:用不同的溶剂对枳实根进行浸提。硅胶柱层析，乙酸乙酯萃取物经正己烷:乙酸乙酯(4:8)洗脱，得到黄色无定形固体。结果:成功地从苦楝根中分离到一种独特的香叶黄酮。结构为8-(2′，3′-环氧-3′-甲基丁基)-4′-丙烯氧基黄酮-7- o -6′-乙酰基β- d -葡萄糖吡喃苷。结论:根据光谱分析结果，从柠檬草中分离到一种未知的黄酮。
{"title":"Geranyl flavanone from Feronia limonia (L.) Swingle","authors":"Sumia Urai̇nab, S. Kamal, Sevinj Musayeva Vagif, Arifa Mahreen, Saba Zafar","doi":"10.2478/hepo-2022-0022","DOIUrl":"https://doi.org/10.2478/hepo-2022-0022","url":null,"abstract":"Summary Introduction: Feronia limonia (synonyms: Feronia elephantum, Hesperethusa crenulata, Limonia acidissima Linn, Schinus limonia) is a member of Rutaceae family. F. limonia is an aesthetically pleasing plant with various medicinal and nutraceutical properties. Objective: The primary objective of this research was to determine the flavonoid from F. limonia. Methods: Different solvents have been soxhleted to the roots of F. limonia powder. Column chromatography was performed using silica gel, a yellow amorphous solid was obtained from ethyl acetate extract after elution with hexane: ethyl acetate (4:8). Results: We have successfully isolated a unique geranyl flavanone from the roots of F. limonia. The structure was verified as 8-(2′′,3′′-epoxy-3′′-methyl butyl)-4′-prenyloxy flavanone-7-O-6′′′′-acetyl β-D-glucopyranoside. Conclusion: According to the findings based upon spectroscopic analysis, a hitherto unknown flavanone was isolated in the F. limonia.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"68 1","pages":"20 - 24"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44392320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Introduction: The technological advancements in the production of synthetic seeds are critical for the preservation of valuable genotypes of many herbal plants, including Salvia officinalis – sage. Objective: The aim of this study was the production, storage and conversion of artificial sage seeds. The technology of synthetic seeds is placing explants capable of regeneration into plants in a protective casing. Methods: Apical and axillary buds were encapsulated with 1.2% sodium alginate solution, and then dripped in 200 mM CaCl2 solution. Artificial seeds were stored at 4°C for 30 days and then converted on MS medium containing 0.3 mg/l of BAP. Results: The synthetic seeds technology made it possible to obtain a high level of seeds conversion into plants using apical buds (85.0%), and slightly lower in the case of side buds (62.5%). Conclusion: The fully developed technology of synthetic seeds made it possible to obtain a high level of plant viability, which may prove useful for the storage of valuable genotypes of sage.
摘要简介:合成种子生产技术的进步对于保存包括鼠尾草在内的许多有价值的草本植物基因型至关重要。目的:研究人工鼠尾草种子的生产、储存和转化。人工合成种子的技术是将能够再生的外植体植入植物的保护性外壳中。方法:用1.2%海藻酸钠溶液包封根尖芽和腋芽，然后滴入200 mM CaCl2溶液。人工种子在4℃下保存30 d，然后在含0.3 mg/l BAP的MS培养基上转化。结果:人工合成种子技术使种子转化成植株的最高转化率达到85.0%，而侧芽的转化率略低(62.5%)。结论:成熟的合成种子技术使合成种子获得较高的植物活力成为可能，为保存有价值的鼠尾草基因型提供了可能。
{"title":"Synthetic seeds technology of Salvia officinalis as a method for short-term storage and in vitro propagation of valuable genotypes","authors":"Aleksandra Deja","doi":"10.2478/hepo-2022-0024","DOIUrl":"https://doi.org/10.2478/hepo-2022-0024","url":null,"abstract":"Summary Introduction: The technological advancements in the production of synthetic seeds are critical for the preservation of valuable genotypes of many herbal plants, including Salvia officinalis – sage. Objective: The aim of this study was the production, storage and conversion of artificial sage seeds. The technology of synthetic seeds is placing explants capable of regeneration into plants in a protective casing. Methods: Apical and axillary buds were encapsulated with 1.2% sodium alginate solution, and then dripped in 200 mM CaCl2 solution. Artificial seeds were stored at 4°C for 30 days and then converted on MS medium containing 0.3 mg/l of BAP. Results: The synthetic seeds technology made it possible to obtain a high level of seeds conversion into plants using apical buds (85.0%), and slightly lower in the case of side buds (62.5%). Conclusion: The fully developed technology of synthetic seeds made it possible to obtain a high level of plant viability, which may prove useful for the storage of valuable genotypes of sage.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"68 1","pages":"25 - 29"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43981421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Introduction: Drought stress is known to change biochemical activities resulting in altered plant growth and development. Objective: Due to the shortage of research evidence in a certain taxa of basil, it is imperative to examine the relationship between drought stress and morphological changes as well as essential oil accumulation. Methods: An open field experiment was conducted in 2020 to determine the effects of drought stress on the morphological and biochemical responses of selected Ocimum species. Five basil species, namely O. basilicum ‘Genovese’, O. basilicum ‘Ohre’, O. × africanum, O. americanum, O. selloi, and O. sanctum ‘Krishna’ were grown under irrigated (control) and non-irrigated (drought stress treatment) plots with two block replications. Results: The result showed that irrigation had a positive effect on production. Consequently, fresh and dry biomass yields were higher on irrigated plots by 39% and 33%, respectively. No significant changes were detected between the treatments in essential oils (EO) yield and EO composition of major compounds. However, a slight increase in camphor (O. × africanum), nerol (O. americanum), and trans-β-caryophyllene (O. sanctum ‘Krishna’) ratios were observed under irrigation treatments. On the other hand, drought stress increased EO content, polyphenol content, and antioxidant capacity. Furthermore, drought stress had a positive effect on the ratios of 1,8-cineole (O. × africanum), and eugenol (O. sanctum ‘Krishna’). There is only a slight increase in EO content under drought stress, which is not comparable to the significant yield reduction recorded. Morphological and biochemical variations were also detected among basil species. Accordingly, higher biomass (616.33 g/plant) and EO yield (3.72 ml/100 g) among the species were obtained from O. basilicum ‘Genovese’ and O. × africanum, respectively. Conclusion: Irrigation is imperative for maximizing biomass and EO yield of tested basil species.
{"title":"Morphological and biochemical responses of selected Ocimum species under drought","authors":"S. Mulugeta, B. Gosztola, P. Radácsi","doi":"10.2478/hepo-2022-0019","DOIUrl":"https://doi.org/10.2478/hepo-2022-0019","url":null,"abstract":"Summary Introduction: Drought stress is known to change biochemical activities resulting in altered plant growth and development. Objective: Due to the shortage of research evidence in a certain taxa of basil, it is imperative to examine the relationship between drought stress and morphological changes as well as essential oil accumulation. Methods: An open field experiment was conducted in 2020 to determine the effects of drought stress on the morphological and biochemical responses of selected Ocimum species. Five basil species, namely O. basilicum ‘Genovese’, O. basilicum ‘Ohre’, O. × africanum, O. americanum, O. selloi, and O. sanctum ‘Krishna’ were grown under irrigated (control) and non-irrigated (drought stress treatment) plots with two block replications. Results: The result showed that irrigation had a positive effect on production. Consequently, fresh and dry biomass yields were higher on irrigated plots by 39% and 33%, respectively. No significant changes were detected between the treatments in essential oils (EO) yield and EO composition of major compounds. However, a slight increase in camphor (O. × africanum), nerol (O. americanum), and trans-β-caryophyllene (O. sanctum ‘Krishna’) ratios were observed under irrigation treatments. On the other hand, drought stress increased EO content, polyphenol content, and antioxidant capacity. Furthermore, drought stress had a positive effect on the ratios of 1,8-cineole (O. × africanum), and eugenol (O. sanctum ‘Krishna’). There is only a slight increase in EO content under drought stress, which is not comparable to the significant yield reduction recorded. Morphological and biochemical variations were also detected among basil species. Accordingly, higher biomass (616.33 g/plant) and EO yield (3.72 ml/100 g) among the species were obtained from O. basilicum ‘Genovese’ and O. × africanum, respectively. Conclusion: Irrigation is imperative for maximizing biomass and EO yield of tested basil species.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"68 1","pages":"1 - 10"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45161980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Van, T. Thang, Thi Hang Nguyen, Nhat Tuong Huynh, T. Pham, L. Quoc, N. Trinh, Van-Son Le, Q. Nguyen, T. Le, Nga Nguyen-Phi
Summary Introduction: Curcuma xanthella Škorničk. is a rare species. Recently, it has been described as a new species from Vietnam. Objective: In this study, the chemical composition of the acetone extract as well as the antibacterial and antioxidant effects of acetone extract and its fractions such as n-hexane, chloroform and ethyl acetate of C. xanthella were investigated for the first time. Methods: The chemical components obtained from the acetone extract of C. xanthella were performed by using gas chromatography/mass spectrometry assays. Disc diffusion assay was used to assess the antibacterial effects of studied samples. Free radical scavenging ability of the extracts was determined by DPPH free radical scavenging (DPPH RSA) assay. Results: Sixty chemical compounds were reported from C. xanthella, of which 1,8-cineole (11.3%), n-hexadecanoic acid (11.13%), stigmasterol (8.2%), γ-sitosterol (6.78%) and neophytadiene (6.71%) were the major compounds. The acetone extract and its fractions such as n-hexane and ethyl acetate were found to be effective against eight oral bacteria, including Escherichia coli, Klebsiella pneumoniae, Shigella flexneri, Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa, Bacillus cereus and Staphylococcus aureus. The acetone extract and its fractions such as n-hexane, chloroform and ethyl acetate of C. xanthella showed DPPH radical scavenging activity with IC50 value of 6.60, 2.38, 8.45 and 3.42 mg/ml, respectively. Conclusion: Sixty chemical constituents were identified in the acetone extract of C. xanthella. The acetone extract and its fractions showed antibacterial effect and DPPH radical scavenging activity.
{"title":"Chemical profiles and biological activities of acetone extracts of Curcuma xanthella","authors":"H. Van, T. Thang, Thi Hang Nguyen, Nhat Tuong Huynh, T. Pham, L. Quoc, N. Trinh, Van-Son Le, Q. Nguyen, T. Le, Nga Nguyen-Phi","doi":"10.2478/hepo-2022-0021","DOIUrl":"https://doi.org/10.2478/hepo-2022-0021","url":null,"abstract":"Summary Introduction: Curcuma xanthella Škorničk. is a rare species. Recently, it has been described as a new species from Vietnam. Objective: In this study, the chemical composition of the acetone extract as well as the antibacterial and antioxidant effects of acetone extract and its fractions such as n-hexane, chloroform and ethyl acetate of C. xanthella were investigated for the first time. Methods: The chemical components obtained from the acetone extract of C. xanthella were performed by using gas chromatography/mass spectrometry assays. Disc diffusion assay was used to assess the antibacterial effects of studied samples. Free radical scavenging ability of the extracts was determined by DPPH free radical scavenging (DPPH RSA) assay. Results: Sixty chemical compounds were reported from C. xanthella, of which 1,8-cineole (11.3%), n-hexadecanoic acid (11.13%), stigmasterol (8.2%), γ-sitosterol (6.78%) and neophytadiene (6.71%) were the major compounds. The acetone extract and its fractions such as n-hexane and ethyl acetate were found to be effective against eight oral bacteria, including Escherichia coli, Klebsiella pneumoniae, Shigella flexneri, Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa, Bacillus cereus and Staphylococcus aureus. The acetone extract and its fractions such as n-hexane, chloroform and ethyl acetate of C. xanthella showed DPPH radical scavenging activity with IC50 value of 6.60, 2.38, 8.45 and 3.42 mg/ml, respectively. Conclusion: Sixty chemical constituents were identified in the acetone extract of C. xanthella. The acetone extract and its fractions showed antibacterial effect and DPPH radical scavenging activity.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"2019 1","pages":"30 - 38"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69210233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Introduction: Herbs or plants are used for a variety of purposes, including nutrition, medicinal and in the beverage industry, beverages, coloring, food preservatives, insect repellants and cosmetics. Methods: Many plants were obtained from local markets and the oil was extracted with hexane at a concentration of 75%. Green silver nanoparticles were prepared. The nanoparticles were characterized using various techniques, including scanning electron microscopy, UV visible spectroscopy, Fourier transform infra-red, energy dispersive spectroscopy, and zeta potential analyzer. Results: The results showed small, smooth spherical nanoparticles, ranging between 57.41–88.00 nm, as well as the distribution of electric charges evenly on the surface of the nanoparticles, which acquired effective agents for nanoparticles against aflatoxin-b1. The effectiveness of green nanoparticles against aflatoxin-b1 by using high-performance liquid chromatography technology detected its concentration. The standard concentration of aflatoxin-b1 was (20) ppb. The results of the activity of plant oil extracts of T-thyme, rosemary-R, mint-M and eucalyptus-E reached (0.104, 1.586, 1.083 and 1.067) ppb, while it appears in the nanoparticle activity of T, R, M and E were as (0.065, 0.226, 0.377 and 0.702) ppb respectively. Conclusion: We concluded that green Ag nanoparticles are efficient in processing or eliminating aflatoxin-b1 and can be produced at very low concentrations compared to the concentrations of plant extracts prepared.
{"title":"Evaluation of detoxification of aflatoxin-b1 by using Ag nanoparticles of oil extracts user prepared by using some medical herbs","authors":"Mokdad. M. Jawad, H. Attiya, L. A. Al-Zubaidi","doi":"10.2478/hepo-2022-0020","DOIUrl":"https://doi.org/10.2478/hepo-2022-0020","url":null,"abstract":"Summary Introduction: Herbs or plants are used for a variety of purposes, including nutrition, medicinal and in the beverage industry, beverages, coloring, food preservatives, insect repellants and cosmetics. Methods: Many plants were obtained from local markets and the oil was extracted with hexane at a concentration of 75%. Green silver nanoparticles were prepared. The nanoparticles were characterized using various techniques, including scanning electron microscopy, UV visible spectroscopy, Fourier transform infra-red, energy dispersive spectroscopy, and zeta potential analyzer. Results: The results showed small, smooth spherical nanoparticles, ranging between 57.41–88.00 nm, as well as the distribution of electric charges evenly on the surface of the nanoparticles, which acquired effective agents for nanoparticles against aflatoxin-b1. The effectiveness of green nanoparticles against aflatoxin-b1 by using high-performance liquid chromatography technology detected its concentration. The standard concentration of aflatoxin-b1 was (20) ppb. The results of the activity of plant oil extracts of T-thyme, rosemary-R, mint-M and eucalyptus-E reached (0.104, 1.586, 1.083 and 1.067) ppb, while it appears in the nanoparticle activity of T, R, M and E were as (0.065, 0.226, 0.377 and 0.702) ppb respectively. Conclusion: We concluded that green Ag nanoparticles are efficient in processing or eliminating aflatoxin-b1 and can be produced at very low concentrations compared to the concentrations of plant extracts prepared.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"68 1","pages":"11 - 19"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43577306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. H. Nurani, E. Darmawan, Akrom, A. Guntarti, Warsi, C. A. Edityaningrum, N. Harun, Dini Mardhiyani, Nur Azizah Syahrana, N. Azizah, Siti Setianingsih, A. Rohman
Summary Introduction: Rosella (Hibiscus sabdariffa L) extract is often used as immune-stimulant because it contains flavonoids, especially anthocyanin and quercetin with antioxidant activities. Objective: This study aimed to determine the safety of the rosella extract consumed in the form of capsules on the vital signs, haematologic parameters as well as kidney and liver function. Methods: This research was conducted using clinical trial pre- and post-test design in healthy participants. There were 21 healthy participants (52% male, age ranged 8–45) consuming rosella capsules for thirty days, in a dose of 500 mg extract daily. Leukocytes, lymphocytes, blood urea nitrogen (BUN), serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and vital signs were consecutively evaluated on days 0, 31, and 45, respectively. The Wilcoxon and paired sample t-test were used to compare the parameters among the evaluated times. Results: The result showed that no significant difference for all parameters among the three time points (p>0.05). Conclusion: These findings suggested that the administration of ethanolic extract of rosella is potential safe and does not negatively affect the vital signs, haemoglobin, leukocytes, lymphocytes, BUN, SGOT, and SGPT.
{"title":"Effect of ethanolic extract of rosella (Hibiscus sabdariffa L.) on vital signs, kidney, and liver safety","authors":"L. H. Nurani, E. Darmawan, Akrom, A. Guntarti, Warsi, C. A. Edityaningrum, N. Harun, Dini Mardhiyani, Nur Azizah Syahrana, N. Azizah, Siti Setianingsih, A. Rohman","doi":"10.2478/hepo-2022-0018","DOIUrl":"https://doi.org/10.2478/hepo-2022-0018","url":null,"abstract":"Summary Introduction: Rosella (Hibiscus sabdariffa L) extract is often used as immune-stimulant because it contains flavonoids, especially anthocyanin and quercetin with antioxidant activities. Objective: This study aimed to determine the safety of the rosella extract consumed in the form of capsules on the vital signs, haematologic parameters as well as kidney and liver function. Methods: This research was conducted using clinical trial pre- and post-test design in healthy participants. There were 21 healthy participants (52% male, age ranged 8–45) consuming rosella capsules for thirty days, in a dose of 500 mg extract daily. Leukocytes, lymphocytes, blood urea nitrogen (BUN), serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT) and vital signs were consecutively evaluated on days 0, 31, and 45, respectively. The Wilcoxon and paired sample t-test were used to compare the parameters among the evaluated times. Results: The result showed that no significant difference for all parameters among the three time points (p>0.05). Conclusion: These findings suggested that the administration of ethanolic extract of rosella is potential safe and does not negatively affect the vital signs, haemoglobin, leukocytes, lymphocytes, BUN, SGOT, and SGPT.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"68 1","pages":"8 - 16"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49619631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary In Poland, food supplements have been popular among consumers for many years. Their sale brings producers huge profits. The interest in these products, especially in vitamin and mineral preparations and in immunity-supporting products increased dramatically due to the COVID-19 pandemic. Self-medication with untested dietary supplements may be health hazardous. Since in Poland and in other countries the procedures of quality control of food supplements are imperfect, this situation raises serious concerns about the risks of their unrestricted use and food fraud. The article briefly presents important problems in the safety of food supplements from the point of view of experts involved in their evaluation.
{"title":"Food supplements in Poland in context of issues related to their safety as food","authors":"Justyna Baraniak, Małgorzata Kania-Dobrowolska, Radosław Kujawski","doi":"10.2478/hepo-2022-0015","DOIUrl":"https://doi.org/10.2478/hepo-2022-0015","url":null,"abstract":"Summary In Poland, food supplements have been popular among consumers for many years. Their sale brings producers huge profits. The interest in these products, especially in vitamin and mineral preparations and in immunity-supporting products increased dramatically due to the COVID-19 pandemic. Self-medication with untested dietary supplements may be health hazardous. Since in Poland and in other countries the procedures of quality control of food supplements are imperfect, this situation raises serious concerns about the risks of their unrestricted use and food fraud. The article briefly presents important problems in the safety of food supplements from the point of view of experts involved in their evaluation.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"68 1","pages":"36 - 42"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47255480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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