Summary Introduction The Institute of Natural Fibres and Medicinal Plants National Research Institute (IWNiRZ) – formerly Institute of Natural Fibres, together with Plant Breeding and Acclimatization Institute (IHAR) in Radzików carry out tasks related to the protection of genetic resources of flax (Linum usitatissimum L.). Genetic resources of flax are stored as seeds in IHAR refrigeration chambers. The research material consisted of passport data of genetic resources of flax. Objective The aim of the presenting the identification of duplicates of accessions of flax in documentation of the National Center of Plant Genetic Resources (IHAR). This will facilitate the management of the collection of flax genetic resources. Removal of duplicate accessions from the collection of flax genetic resources will save storage space in long-term chambers, savings in storage costs, savings in working hours. Methods The working methods were studying the passport database and the institute’s gene bank documentation. Results In the cooling chambers of the seed bank at KCRZG at IHAR, unintentional duplicates are stored: 29 flax accessions with the same accessions number and 19 flax accessions with two different accession numbers. Conclusions The presentation in the article of two types of duplicates of flax accessions is very helpful in managing the collection of genetic resources of flax. Reducing the number of genotypes in the flax collection will facilitate the faster collection of all valorization data, which will be very helpful for users of the collection of genetic resources of flax.
{"title":"Selection of duplicates of flax accessions – an important task in the management of collection of genetic resources of Linum usitatissimum L.","authors":"G. Silska","doi":"10.2478/hepo-2021-0027","DOIUrl":"https://doi.org/10.2478/hepo-2021-0027","url":null,"abstract":"Summary Introduction The Institute of Natural Fibres and Medicinal Plants National Research Institute (IWNiRZ) – formerly Institute of Natural Fibres, together with Plant Breeding and Acclimatization Institute (IHAR) in Radzików carry out tasks related to the protection of genetic resources of flax (Linum usitatissimum L.). Genetic resources of flax are stored as seeds in IHAR refrigeration chambers. The research material consisted of passport data of genetic resources of flax. Objective The aim of the presenting the identification of duplicates of accessions of flax in documentation of the National Center of Plant Genetic Resources (IHAR). This will facilitate the management of the collection of flax genetic resources. Removal of duplicate accessions from the collection of flax genetic resources will save storage space in long-term chambers, savings in storage costs, savings in working hours. Methods The working methods were studying the passport database and the institute’s gene bank documentation. Results In the cooling chambers of the seed bank at KCRZG at IHAR, unintentional duplicates are stored: 29 flax accessions with the same accessions number and 19 flax accessions with two different accession numbers. Conclusions The presentation in the article of two types of duplicates of flax accessions is very helpful in managing the collection of genetic resources of flax. Reducing the number of genotypes in the flax collection will facilitate the faster collection of all valorization data, which will be very helpful for users of the collection of genetic resources of flax.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"70 - 76"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44374824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmad Sabry Mohamad, Muhammad Noor Nordin, Ismail Che Ani, Johan Jemberang, R. Ishak, A. Hasan, Muhammad Imran Ahmad, Mohd Rahman Omar, Roszymah Hamzah
Summary Introduction Volten VR4® capsules containing herb extract of Kaempferia parviflora has been claimed to reduce blood glucose in patients with diabetes. Objectives This preliminary study is conducted to evaluate the efficacy of Volten VR4® on healthy individuals and type-2 diabetes mellitus volunteers. The extracts of 400 mg capsules of Kaempferia parviflora (KP) were used to measure the blood glucose level of 2-hour postprandial. Methods The healthy group consists of 15 young adults aged 20-30 with no history of serious diseases, while the diabetic group includes 12 individuals aged 35-75 diagnosed with type-2 diabetes mellitus. Data were validated through the Willcoxon and Friedman test statistics and error distribution. The investigation was continued to trace the capsules contents of elements using inductively coupled plasma optical emission spectrometry (ICP-OES) techniques. Results It has been shown that KP reducing blood sugar levels has been associated with flavonoids and methoxyflavones components. The result specifically showed that consuming VR4® capsules can significantly reduce blood glucose, either at the state of fasting or postprandially. In the study the content of mineral and heavy metal elements in VR4® capsules has been evaluated. Conclusion Volten VR4® Kaempferia parviflora extract is safe to be consumed at a single dose of 400 mg. The study also has shown that the participants are free from adverse reactions and hypoglycaemia.
{"title":"Preliminary studies of Volten VR4® Kaempferia parviflora herb extracts on blood glucose levels in human type-2 diabetes mellitus and its mineral element analysis","authors":"Ahmad Sabry Mohamad, Muhammad Noor Nordin, Ismail Che Ani, Johan Jemberang, R. Ishak, A. Hasan, Muhammad Imran Ahmad, Mohd Rahman Omar, Roszymah Hamzah","doi":"10.2478/hepo-2021-0021","DOIUrl":"https://doi.org/10.2478/hepo-2021-0021","url":null,"abstract":"Summary Introduction Volten VR4® capsules containing herb extract of Kaempferia parviflora has been claimed to reduce blood glucose in patients with diabetes. Objectives This preliminary study is conducted to evaluate the efficacy of Volten VR4® on healthy individuals and type-2 diabetes mellitus volunteers. The extracts of 400 mg capsules of Kaempferia parviflora (KP) were used to measure the blood glucose level of 2-hour postprandial. Methods The healthy group consists of 15 young adults aged 20-30 with no history of serious diseases, while the diabetic group includes 12 individuals aged 35-75 diagnosed with type-2 diabetes mellitus. Data were validated through the Willcoxon and Friedman test statistics and error distribution. The investigation was continued to trace the capsules contents of elements using inductively coupled plasma optical emission spectrometry (ICP-OES) techniques. Results It has been shown that KP reducing blood sugar levels has been associated with flavonoids and methoxyflavones components. The result specifically showed that consuming VR4® capsules can significantly reduce blood glucose, either at the state of fasting or postprandially. In the study the content of mineral and heavy metal elements in VR4® capsules has been evaluated. Conclusion Volten VR4® Kaempferia parviflora extract is safe to be consumed at a single dose of 400 mg. The study also has shown that the participants are free from adverse reactions and hypoglycaemia.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"42 - 53"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43839839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Increasing knowledge of the gut microbiota and its interference in human homeostasis in recent years has contributed to a better understanding of number of different interactions occurring in the gastrointestinal tract. Disruption of the microbiota is detrimental to health and contributes to the development of numerous diseases and may also be an accelerator of pathophysiological processes such as atherosclerosis. Cardiovascular diseases are the most common cause of death worldwide, so the development of new methods to support the treatment and prevention of these diseases becoms one of the priorities of modern medicine. Probiotics may constitute an important element of support in the treatment and prevention of CVD (cardiovascular diseases). A number of papers support such a statement, however, larger clinical trials are needed. Through a number of mechanisms including mitigating inflammation, sealing the intestinal epithelium, and affecting metabolism, probiotics may have a beneficial effect on general health and slow down the pathogenesis of many diseases, including those affecting the cardiovascular system. This article contains a review of current discoveries on the role of probiotics in the prevention and support of CVD treatment.
{"title":"Probiotics in prevention and treatment of cardiovascular diseases","authors":"Filip Przerwa, A. Kukowka, K. Kotrych, I. Uzar","doi":"10.2478/hepo-2021-0026","DOIUrl":"https://doi.org/10.2478/hepo-2021-0026","url":null,"abstract":"Summary Increasing knowledge of the gut microbiota and its interference in human homeostasis in recent years has contributed to a better understanding of number of different interactions occurring in the gastrointestinal tract. Disruption of the microbiota is detrimental to health and contributes to the development of numerous diseases and may also be an accelerator of pathophysiological processes such as atherosclerosis. Cardiovascular diseases are the most common cause of death worldwide, so the development of new methods to support the treatment and prevention of these diseases becoms one of the priorities of modern medicine. Probiotics may constitute an important element of support in the treatment and prevention of CVD (cardiovascular diseases). A number of papers support such a statement, however, larger clinical trials are needed. Through a number of mechanisms including mitigating inflammation, sealing the intestinal epithelium, and affecting metabolism, probiotics may have a beneficial effect on general health and slow down the pathogenesis of many diseases, including those affecting the cardiovascular system. This article contains a review of current discoveries on the role of probiotics in the prevention and support of CVD treatment.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"77 - 85"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44395210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Nowak, A. Kiss, C. Wambebe, E. Katuura, Ł. Kuźma
Summary Introduction Carica papaya L. belongs to Caricaceae family. It is an evergreen tree or shrub with usually unbranched trunk. Previous studies on the methanolic leaf extract of this plant proved the antibacterial, cytotoxic, anticancer and antioxidant effects indicative of promising therapeutic potentials. Methods Chromatographic and spectroscopic techniques along with high performance liquid chromatography quantitative analysis were performed to isolate, tentatively identify and quantify polyphenolic compounds in MeOH extract of C. papaya leaves. UHPLC-DAD-ESI-MS/MS and UHPLC-DAD methods were adopted for qualitative and quantitative analysis respectively. In the case of polyphenol separation, some reference substances were isolated by preparative HPLC. Results Six polyphenols were isolated and quantified from C. papaya leaf extract, including three phenolic acids: derivatives of caffeic acid, p-coumaric acid, ferulic acid and three flavonoids: quercetin dirhamnosylhexoside, kaempferol dirhamnosyl-hexoside, quercetin 3-O-rutinoside. Among them quercetin dirhamnosyl-hexoside was significantly higher (1.9 mg/g extract) than other identified compounds. Conclusion The results of present study provide a new data on polyphenols composition of C. papaya plant derived from Uganda and promote research on its possible standardization and use as a dietary supplement and/or herbal medicine.
{"title":"Tentative qualitative and quantitative analysis of phenolic compounds in leaf extract from Carica papaya Linn. plant growing in Uganda","authors":"J. Nowak, A. Kiss, C. Wambebe, E. Katuura, Ł. Kuźma","doi":"10.2478/hepo-2021-0029","DOIUrl":"https://doi.org/10.2478/hepo-2021-0029","url":null,"abstract":"Summary Introduction Carica papaya L. belongs to Caricaceae family. It is an evergreen tree or shrub with usually unbranched trunk. Previous studies on the methanolic leaf extract of this plant proved the antibacterial, cytotoxic, anticancer and antioxidant effects indicative of promising therapeutic potentials. Methods Chromatographic and spectroscopic techniques along with high performance liquid chromatography quantitative analysis were performed to isolate, tentatively identify and quantify polyphenolic compounds in MeOH extract of C. papaya leaves. UHPLC-DAD-ESI-MS/MS and UHPLC-DAD methods were adopted for qualitative and quantitative analysis respectively. In the case of polyphenol separation, some reference substances were isolated by preparative HPLC. Results Six polyphenols were isolated and quantified from C. papaya leaf extract, including three phenolic acids: derivatives of caffeic acid, p-coumaric acid, ferulic acid and three flavonoids: quercetin dirhamnosylhexoside, kaempferol dirhamnosyl-hexoside, quercetin 3-O-rutinoside. Among them quercetin dirhamnosyl-hexoside was significantly higher (1.9 mg/g extract) than other identified compounds. Conclusion The results of present study provide a new data on polyphenols composition of C. papaya plant derived from Uganda and promote research on its possible standardization and use as a dietary supplement and/or herbal medicine.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"1 - 9"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46999346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Bensaad, S. Dassamiour, L. Hambaba, C. Bensouici, Ouffroukh Karima, M. Kahoul
Summary Introduction Traditional medicine has an important place in human history and this since antiquity. Indeed, during Egyptian and Chinese civilization era, many detailed manuscripts, describing the therapeutic effect of plants, were found which suggest that folk medicine is the basis of the actual medicine. Objective To investigate the phytochemical and pharmacological properties of the n-butanol (n-BuOH) and ethyl acetate (EA) extracts of the aerial part of Centaurea tougourensis. Methods The phytochemical evaluation was done based on HPLC-DAD approach. The antioxidant activity was determined by DPPH and cupric ion reducing antioxidant capacity (CUPRAC), while the hemostatic effect was performed using plasma recalcification time (PRT) method. The antidiabetic capacity was investigated by alpha-amylase inhibition assay and the photoprotective test was evaluated by the measurement of sun protection factor (SPF). Results 13 phenolic compounds were identified in both extracts of C. tougourensis. These extracts showed antioxidant, haemostatic, antidiabetic and photoprotective properties with a dose-dependent manner. Amounts of n-BuOH activities were found higher, with a respective IC50 value of 0.72±0.07 μg/ml in DPPH assay, an A0.50 value lower than 3.125 μg/ml in CUPRAC assay besides a shortening rate percentage of coagulation (86.71%) in haemostatic assay, a moderate inhibition effect on alpha amylase activity with an IC50 value of (711.5±0.03 μg/ml) and a maximum sun protection factor of (56.035). These results were mostly found highly significant (p<0.001) when compared to respective standards. Conclusion This study demonstrated some pharmacological effects of C. tougourensis which suggests that our plant could be a good candidate to treat some illnesses related to oxidative stress, bleeding or skin cancer.
{"title":"HPLC-DAD phenolics screening and in vitro investigation of haemostatic, antidiabetic, antioxidant and photoprotective properties of Centaurea tougourensis Boiss. & Reut.","authors":"M. Bensaad, S. Dassamiour, L. Hambaba, C. Bensouici, Ouffroukh Karima, M. Kahoul","doi":"10.2478/hepo-2021-0023","DOIUrl":"https://doi.org/10.2478/hepo-2021-0023","url":null,"abstract":"Summary Introduction Traditional medicine has an important place in human history and this since antiquity. Indeed, during Egyptian and Chinese civilization era, many detailed manuscripts, describing the therapeutic effect of plants, were found which suggest that folk medicine is the basis of the actual medicine. Objective To investigate the phytochemical and pharmacological properties of the n-butanol (n-BuOH) and ethyl acetate (EA) extracts of the aerial part of Centaurea tougourensis. Methods The phytochemical evaluation was done based on HPLC-DAD approach. The antioxidant activity was determined by DPPH and cupric ion reducing antioxidant capacity (CUPRAC), while the hemostatic effect was performed using plasma recalcification time (PRT) method. The antidiabetic capacity was investigated by alpha-amylase inhibition assay and the photoprotective test was evaluated by the measurement of sun protection factor (SPF). Results 13 phenolic compounds were identified in both extracts of C. tougourensis. These extracts showed antioxidant, haemostatic, antidiabetic and photoprotective properties with a dose-dependent manner. Amounts of n-BuOH activities were found higher, with a respective IC50 value of 0.72±0.07 μg/ml in DPPH assay, an A0.50 value lower than 3.125 μg/ml in CUPRAC assay besides a shortening rate percentage of coagulation (86.71%) in haemostatic assay, a moderate inhibition effect on alpha amylase activity with an IC50 value of (711.5±0.03 μg/ml) and a maximum sun protection factor of (56.035). These results were mostly found highly significant (p<0.001) when compared to respective standards. Conclusion This study demonstrated some pharmacological effects of C. tougourensis which suggests that our plant could be a good candidate to treat some illnesses related to oxidative stress, bleeding or skin cancer.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"16 - 31"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42965996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joanna Sucholas, M. Ukhanova, Anja Greinwald, R. Luick
Summary Introduction: Poland is one of the main exporters of medicinal and aromatic plants (MAPs) in Europe. A substantial part of this plant material is collected in the wild. So far, the system of wild collection (WC) of medicinal plants in Poland, its social and ecological aspects have not been studied comprehensively. Objective: The aim of the study was to analyse the organisation of WC system in Poland, including legislation, situation of protected species, and socio-ecological dimensions of the MAPs’ supply chains. Methods: Data from environmental agencies (RDOŚ) about protected MAPs was analysed. Stakeholders engaged in WC were interviewed. National legislation on WC was reviewed. Results: Although Poland is the second largest exporter of MAPs in Europe, legislation on wild collection in the country is sparse and incoherent. Monitoring does not apply to WC entirely. The structure of WC has a large degree of informality, lacks transparency, and does not take many sustainability criteria into account. Conclusions: The system needs major adjustments: a) to insure sustainability of WC, b) to accommodate market demands, including plant material quality.
{"title":"Wild collection of medicinal and aromatic plants (MAPs) for commercial purposes in Poland - a system’s analysis","authors":"Joanna Sucholas, M. Ukhanova, Anja Greinwald, R. Luick","doi":"10.2478/hepo-2021-0014","DOIUrl":"https://doi.org/10.2478/hepo-2021-0014","url":null,"abstract":"Summary Introduction: Poland is one of the main exporters of medicinal and aromatic plants (MAPs) in Europe. A substantial part of this plant material is collected in the wild. So far, the system of wild collection (WC) of medicinal plants in Poland, its social and ecological aspects have not been studied comprehensively. Objective: The aim of the study was to analyse the organisation of WC system in Poland, including legislation, situation of protected species, and socio-ecological dimensions of the MAPs’ supply chains. Methods: Data from environmental agencies (RDOŚ) about protected MAPs was analysed. Stakeholders engaged in WC were interviewed. National legislation on WC was reviewed. Results: Although Poland is the second largest exporter of MAPs in Europe, legislation on wild collection in the country is sparse and incoherent. Monitoring does not apply to WC entirely. The structure of WC has a large degree of informality, lacks transparency, and does not take many sustainability criteria into account. Conclusions: The system needs major adjustments: a) to insure sustainability of WC, b) to accommodate market demands, including plant material quality.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"1 - 18"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43037639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Rubio-Sanz, C. Dorca-Fornell, M. Fornos, E. Navarro-León, M. Jaizme-Vega
Summary Introduction: Moringa oleifera (moringa) is a fast-growing tree from north India and Himalayan foothills considered to be one of the most nutritious species. Especially it’s leaves contain proteins in a high concentration, all essential amino acids, nutrients like calcium, iron, potassium and cooper, vitamin A, E and group B, and also antioxidants and polyphenols. Many studies have demonstrated the medicinal properties of moringa leaves consumption thanks to its antioxidant, anti-inflammatory, prevention of diabetes or antitumor and anticancer properties. The amount and number of essential nutrients and phytochemical constituents may vary depending on the plant tissue and age and especially on the climatic and edaphological conditions. Methods: We pointed to demonstrate moringa plants grown on proper conditions display a better production of its desired compounds. In this work, the mineral, amino acids, antioxidant activity and various bioactive compounds were analysed in moringa leaves extract from plants grown on Tenerife Island under organic production and similar climatic and edaphological conditions as the place of origin. Results: Our results revealed a high concentration of several nutrients as calcium, potassium and iron, as comparison to other moringa plants; β-carotene (pro-vitamin A), ascorbate (vitamin C) and glutathione, whose function is mainly antioxidant; chlorogenic acid with anticancer function; quercetin with antioxidant, anticancer and antidiabetic functions, among others; glucomoringin-isothiocyanate with anti-micro-biological and neuroprotective function. Conclusion: The results obtained in this study placed on Tenerife Island as one of the best suitable places for moringa good quality production and highlight the potential use of moringa novel food for humans and as a biostimulant for plants.
{"title":"Phytochemical characterization of Moringa oleifera leaves","authors":"L. Rubio-Sanz, C. Dorca-Fornell, M. Fornos, E. Navarro-León, M. Jaizme-Vega","doi":"10.2478/hepo-2021-0019","DOIUrl":"https://doi.org/10.2478/hepo-2021-0019","url":null,"abstract":"Summary Introduction: Moringa oleifera (moringa) is a fast-growing tree from north India and Himalayan foothills considered to be one of the most nutritious species. Especially it’s leaves contain proteins in a high concentration, all essential amino acids, nutrients like calcium, iron, potassium and cooper, vitamin A, E and group B, and also antioxidants and polyphenols. Many studies have demonstrated the medicinal properties of moringa leaves consumption thanks to its antioxidant, anti-inflammatory, prevention of diabetes or antitumor and anticancer properties. The amount and number of essential nutrients and phytochemical constituents may vary depending on the plant tissue and age and especially on the climatic and edaphological conditions. Methods: We pointed to demonstrate moringa plants grown on proper conditions display a better production of its desired compounds. In this work, the mineral, amino acids, antioxidant activity and various bioactive compounds were analysed in moringa leaves extract from plants grown on Tenerife Island under organic production and similar climatic and edaphological conditions as the place of origin. Results: Our results revealed a high concentration of several nutrients as calcium, potassium and iron, as comparison to other moringa plants; β-carotene (pro-vitamin A), ascorbate (vitamin C) and glutathione, whose function is mainly antioxidant; chlorogenic acid with anticancer function; quercetin with antioxidant, anticancer and antidiabetic functions, among others; glucomoringin-isothiocyanate with anti-micro-biological and neuroprotective function. Conclusion: The results obtained in this study placed on Tenerife Island as one of the best suitable places for moringa good quality production and highlight the potential use of moringa novel food for humans and as a biostimulant for plants.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"19 - 26"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47433072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radosław Kujawski, M. Szulc, Maria Toboła, M. Graczyk, Kamila Czora-Poczwardowska, Justyna Baraniak, Małgorzata Kania-Dobrowolska, Julia Słyńko-Krzyżostaniak, A. Krajewska-Patan, P. Mikołajczak
Summary Introduction: The molecular basis of in vivo metabolism of selected representatives of phenylethanoids in the presence of ethanol has not been fully elucidated. Objective: The aim was to estimate a salidroside (Sal) metabolism in the liver tissue in rats with induced alcohol tolerance by assessing changes in the transcription of genes encoding cytochromes: CYP1A2, 2D2, 3A1, 2C23. Methods: cDNA was synthesized from total RNA isolated from rat liver samples. mRNA level changes were evaluated using real-time PCR (qRT-PCR) technique. Results: Ethanol caused a significant induction of the CYP1A2 and CYP2C23 genes transcription, and a decrease in the CYP3A1 mRNA level, predominantly without statistical significance. A statistically significant increase of the CYP1A2 mRNA level was observed in the group receiving only Sal (4.5 mg/kg b.w.; p.o.) (p<0.01). Conclusions: There was no unequivocal effect of salidroside on the transcription of investigated cytochrome genes in the liver of rats with induced alcohol tolerance.
{"title":"Lack of salidroside impact on selected cytochromes encoding genes transcription in the liver of ethanol induced rats","authors":"Radosław Kujawski, M. Szulc, Maria Toboła, M. Graczyk, Kamila Czora-Poczwardowska, Justyna Baraniak, Małgorzata Kania-Dobrowolska, Julia Słyńko-Krzyżostaniak, A. Krajewska-Patan, P. Mikołajczak","doi":"10.2478/hepo-2021-0016","DOIUrl":"https://doi.org/10.2478/hepo-2021-0016","url":null,"abstract":"Summary Introduction: The molecular basis of in vivo metabolism of selected representatives of phenylethanoids in the presence of ethanol has not been fully elucidated. Objective: The aim was to estimate a salidroside (Sal) metabolism in the liver tissue in rats with induced alcohol tolerance by assessing changes in the transcription of genes encoding cytochromes: CYP1A2, 2D2, 3A1, 2C23. Methods: cDNA was synthesized from total RNA isolated from rat liver samples. mRNA level changes were evaluated using real-time PCR (qRT-PCR) technique. Results: Ethanol caused a significant induction of the CYP1A2 and CYP2C23 genes transcription, and a decrease in the CYP3A1 mRNA level, predominantly without statistical significance. A statistically significant increase of the CYP1A2 mRNA level was observed in the group receiving only Sal (4.5 mg/kg b.w.; p.o.) (p<0.01). Conclusions: There was no unequivocal effect of salidroside on the transcription of investigated cytochrome genes in the liver of rats with induced alcohol tolerance.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"53 - 65"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45803315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Introduction: Coffee bee pollen contains some precious bioactive compounds, especially phenolic compounds. This material is easily found in many regions in Vietnam and is quite useful for human health. Objective: The main aim of this study was to determine the best extraction conditions for the total polyphenol content (TPC) and antioxidant capacity (AC) of coffee (Coffea robusta L. Linden) bee pollen with microwave-assisted extraction (MAE). Methods: TPC and AC of extract of coffee (C. robusta) bee pollen were determined using Folin-Ciocalteu (FC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. In addition, micromorphology of sample was observed using scanning electron micrographs (SEM). Results: The findings showed that all factors strongly affected the efficiency of the extraction process. The best extraction conditions obtained were ethanol concentration of 60% (v/v), solid to solvent ratio of 1/30 (w/v), extraction time of 7 min, and a microwave power of 314 W. Conclusion: The highest TPC and AC obtained were 13.73 mg GAE/g DW and 10.08 µmol TE/g DW with aqueous ethanol as the solvent at the optimal extraction condition. In addition, the microstructures of the material changed insignificantly under microwave irradiation.
{"title":"Microwave-assisted extraction of phenolic compounds from coffee (Coffea robusta L. Linden) bee pollen","authors":"L. Quoc","doi":"10.2478/hepo-2021-0015","DOIUrl":"https://doi.org/10.2478/hepo-2021-0015","url":null,"abstract":"Summary Introduction: Coffee bee pollen contains some precious bioactive compounds, especially phenolic compounds. This material is easily found in many regions in Vietnam and is quite useful for human health. Objective: The main aim of this study was to determine the best extraction conditions for the total polyphenol content (TPC) and antioxidant capacity (AC) of coffee (Coffea robusta L. Linden) bee pollen with microwave-assisted extraction (MAE). Methods: TPC and AC of extract of coffee (C. robusta) bee pollen were determined using Folin-Ciocalteu (FC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. In addition, micromorphology of sample was observed using scanning electron micrographs (SEM). Results: The findings showed that all factors strongly affected the efficiency of the extraction process. The best extraction conditions obtained were ethanol concentration of 60% (v/v), solid to solvent ratio of 1/30 (w/v), extraction time of 7 min, and a microwave power of 314 W. Conclusion: The highest TPC and AC obtained were 13.73 mg GAE/g DW and 10.08 µmol TE/g DW with aqueous ethanol as the solvent at the optimal extraction condition. In addition, the microstructures of the material changed insignificantly under microwave irradiation.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"37 - 44"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44673558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Introduction: Bauhinia variegata is used in traditional medicine in Pakistan. Objective: The present study was designated to analyze phytochemical screening and cytotoxicity assay of crude aqueous and methanol extracts of flowering buds of Bauhinia variegata. Methods: Qualitative and quantitative phytochemical screening of both extracts were performed by using standard protocols while brine shrimp lethality assay was conducted for cytotoxicity analysis of both extracts. Results: The highest levels of phenolics, flavonoids and terpenoids were observed in methanol extract with respective standard drugs. The aminoacid content was highest followed by phenol and flavonoids in crude aqueous extract of the raw materials. In cytotoxic studies (brine shrimp lethality assay) the use of methanol extract was reported as potent with LD50 of 241.778 µg/ml as compared to aqueous extract with LD50 of 489.7061 µg/ml. Conclusion: This study reveals that the presence of bioactive compounds in both extracts are responsible for the treatment of various ailments and being utilized by the traditional folks as a food as well. And showed that methanol extract possess cytotoxic activity and thus needs further scientific intervention for its value addition.
{"title":"Phytochemical analysis and cytotoxicity evaluation of flowering buds of Bauhinia variegata L.","authors":"Anham Shahid Abbasi, Qazi Najam-Us-Saqib, Atta-ur-Rehman, Nisar-Ur-Rahman","doi":"10.2478/hepo-2021-0013","DOIUrl":"https://doi.org/10.2478/hepo-2021-0013","url":null,"abstract":"Summary Introduction: Bauhinia variegata is used in traditional medicine in Pakistan. Objective: The present study was designated to analyze phytochemical screening and cytotoxicity assay of crude aqueous and methanol extracts of flowering buds of Bauhinia variegata. Methods: Qualitative and quantitative phytochemical screening of both extracts were performed by using standard protocols while brine shrimp lethality assay was conducted for cytotoxicity analysis of both extracts. Results: The highest levels of phenolics, flavonoids and terpenoids were observed in methanol extract with respective standard drugs. The aminoacid content was highest followed by phenol and flavonoids in crude aqueous extract of the raw materials. In cytotoxic studies (brine shrimp lethality assay) the use of methanol extract was reported as potent with LD50 of 241.778 µg/ml as compared to aqueous extract with LD50 of 489.7061 µg/ml. Conclusion: This study reveals that the presence of bioactive compounds in both extracts are responsible for the treatment of various ailments and being utilized by the traditional folks as a food as well. And showed that methanol extract possess cytotoxic activity and thus needs further scientific intervention for its value addition.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"27 - 36"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49535608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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