Hasna Bouhenni, K. Doukani, D. Hanganu, N. Oláh, N. Sekeroglu, S. Gezici, M. Spînu, M. Niculae
Summary Introduction: Natural products represent a gold mine for scientists looking for compounds for the treatment of health problems and diseases with their different biological and pharmacological activities. However, recent research is focused on finding natural sources of antioxidants. Objective: The objective of current research was to determine the phytochemical profile of Algerian fenu-greek (Trigonella foenum-graecum L.), and Syrian cumin (Cuminum cyminum L.) seeds in order to characterize their phenolic compounds and to determine their antioxidant activities. Methods: Total phenolic, flavonoids, condensed and hydrolysable tannins contents were quantified using Folin-Ciocalteu, aluminium chloride, vanillin and ferric chloride methods, respectively. Phenolic compounds were identified by HPLC method and antioxidant activity was measured using DPPH assay. Results: The higher amounts of total phenolic compounds, flavonoids, condensed and hydrolysable tannins were given by fenugreek. Results of HPLC analysis of our plants showed that eight phytochemical compounds were found in cumin extract, and seven molecules in fenugreek extract. Moreover, fenugreek possessed higher antioxidant activity. Conclusion: This study confirmed that our plants are a good source of phenolic contents and possess a high antioxidant activity.
{"title":"Comparative analysis on bioactive compounds and antioxidant activity of Algerian fenugreek (Trigonella foenum-graecum L.) and Syrian cumin (Cuminum cyminum L.) seeds","authors":"Hasna Bouhenni, K. Doukani, D. Hanganu, N. Oláh, N. Sekeroglu, S. Gezici, M. Spînu, M. Niculae","doi":"10.2478/hepo-2021-0005","DOIUrl":"https://doi.org/10.2478/hepo-2021-0005","url":null,"abstract":"Summary Introduction: Natural products represent a gold mine for scientists looking for compounds for the treatment of health problems and diseases with their different biological and pharmacological activities. However, recent research is focused on finding natural sources of antioxidants. Objective: The objective of current research was to determine the phytochemical profile of Algerian fenu-greek (Trigonella foenum-graecum L.), and Syrian cumin (Cuminum cyminum L.) seeds in order to characterize their phenolic compounds and to determine their antioxidant activities. Methods: Total phenolic, flavonoids, condensed and hydrolysable tannins contents were quantified using Folin-Ciocalteu, aluminium chloride, vanillin and ferric chloride methods, respectively. Phenolic compounds were identified by HPLC method and antioxidant activity was measured using DPPH assay. Results: The higher amounts of total phenolic compounds, flavonoids, condensed and hydrolysable tannins were given by fenugreek. Results of HPLC analysis of our plants showed that eight phytochemical compounds were found in cumin extract, and seven molecules in fenugreek extract. Moreover, fenugreek possessed higher antioxidant activity. Conclusion: This study confirmed that our plants are a good source of phenolic contents and possess a high antioxidant activity.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"18 - 34"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47256551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Introduction: Spice plants are a rich source of biologically active compounds. Processes of drying, limits the development of microorganisms and biochemical reactions in raw herbs, at the same time modifies their chemical composition. Objective: The aim of this work was to evaluate the effect of drying process of selected plants on their anti-oxidant potential and the content of biologically active compounds. Methods: The herbal materials thyme herb (Thymus vulgaris L.), sage leaves (Salvia officinalis L.) and rosemary leaves (Rosmarinus officinalis) were dried using two methods: convective (at 30° C), and freeze-drying. In both, fresh and subjected to drying processes herbs, changes in total polyphenol and antioxidant potential (with the DPPH radical), vitamin C (in the form of ascorbic acid) and chlorophyll pigments contents, were studied. Results: The highest content of polyphenols and vitamin C was recorded in rosemary, while sage was characte- rized by the highest content of chlorophylls. It was found that the drying processes caused large losses of vitamin C (ascorbic acid) and dyes in comparison to the fresh material. The highest losses of vitamin C occurred in convection dried sage (95%), while most was preserved in lyophilised thyme (losses 74%). Drying had also influenced the reduction of the content of chlorophylls with the largest loss of these compounds, at 94% observed in the freeze-dried rosemary. However, the content of polyphenols increased, underscoring a large impact on the antioxidant activity of herbs. Conclusions: Drying processes resulted in the release of phenolic compounds, so their content increased three to four times in the case of rosemary and sage. The largest increase was observed in the case of lyophilised thyme. Simultaneously, the antioxidant potential was significantly increased. Herbal droughts proved to be a rich source of antioxidant compounds with promising applications as a food additive.
{"title":"Effect of different drying processes on an antioxidant potential of three species of the Lamiaceae family","authors":"Izabella Kwaśniewska-Karolak, R. Mostowski","doi":"10.2478/hepo-2021-0004","DOIUrl":"https://doi.org/10.2478/hepo-2021-0004","url":null,"abstract":"Summary Introduction: Spice plants are a rich source of biologically active compounds. Processes of drying, limits the development of microorganisms and biochemical reactions in raw herbs, at the same time modifies their chemical composition. Objective: The aim of this work was to evaluate the effect of drying process of selected plants on their anti-oxidant potential and the content of biologically active compounds. Methods: The herbal materials thyme herb (Thymus vulgaris L.), sage leaves (Salvia officinalis L.) and rosemary leaves (Rosmarinus officinalis) were dried using two methods: convective (at 30° C), and freeze-drying. In both, fresh and subjected to drying processes herbs, changes in total polyphenol and antioxidant potential (with the DPPH radical), vitamin C (in the form of ascorbic acid) and chlorophyll pigments contents, were studied. Results: The highest content of polyphenols and vitamin C was recorded in rosemary, while sage was characte- rized by the highest content of chlorophylls. It was found that the drying processes caused large losses of vitamin C (ascorbic acid) and dyes in comparison to the fresh material. The highest losses of vitamin C occurred in convection dried sage (95%), while most was preserved in lyophilised thyme (losses 74%). Drying had also influenced the reduction of the content of chlorophylls with the largest loss of these compounds, at 94% observed in the freeze-dried rosemary. However, the content of polyphenols increased, underscoring a large impact on the antioxidant activity of herbs. Conclusions: Drying processes resulted in the release of phenolic compounds, so their content increased three to four times in the case of rosemary and sage. The largest increase was observed in the case of lyophilised thyme. Simultaneously, the antioxidant potential was significantly increased. Herbal droughts proved to be a rich source of antioxidant compounds with promising applications as a food additive.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"8 - 17"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46682417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary The aim of the article was to obtain maximum information about plant mucilage, its sources and applications in the pharmaceutical industry. This study focuses on the scientific articles and books available in Internet resources and college library that deal with the sources, applications, extraction and isolation of plant mucilage. Mucilage is obtained mainly from plant sources and can be isolated easily. Due to the low cost, easy availability, non-toxicity, non-irritancy, and biocompatibility, mucilage is of great demand in the field of pharmaceuticals. Hibiscus rosa-sinensis L., Trigonella foenum-graecum L., Abelmoschus esculentus L, Plantago ovata Forssk. and Aloe barbadensis L. are some common sources of mucilage. The isolation methods vary depending on the part of the plant where mucilage is present. It is commonly used as gelling agent, suspending agent, binder, and disintegrant. Since it is hydrophilic in nature, chances of deterioration are higher. In this review, different mucilage sources and their isolation methods are discussed in detail. Mucilage is used as excipient in many formulations of tablets, suspensions, gels, etc. The study explores the potential of plant mucilage as an excipient in pharmaceutical formulations. The biodegradable and biocompatible properties of this inexpensive excipient make it more favourable for the newer formulation development.
{"title":"Scope of herbal mucilage in pharmaceutical formulations. A review","authors":"Fels Saju, C. Sivaraman","doi":"10.2478/hepo-2021-0001","DOIUrl":"https://doi.org/10.2478/hepo-2021-0001","url":null,"abstract":"Summary The aim of the article was to obtain maximum information about plant mucilage, its sources and applications in the pharmaceutical industry. This study focuses on the scientific articles and books available in Internet resources and college library that deal with the sources, applications, extraction and isolation of plant mucilage. Mucilage is obtained mainly from plant sources and can be isolated easily. Due to the low cost, easy availability, non-toxicity, non-irritancy, and biocompatibility, mucilage is of great demand in the field of pharmaceuticals. Hibiscus rosa-sinensis L., Trigonella foenum-graecum L., Abelmoschus esculentus L, Plantago ovata Forssk. and Aloe barbadensis L. are some common sources of mucilage. The isolation methods vary depending on the part of the plant where mucilage is present. It is commonly used as gelling agent, suspending agent, binder, and disintegrant. Since it is hydrophilic in nature, chances of deterioration are higher. In this review, different mucilage sources and their isolation methods are discussed in detail. Mucilage is used as excipient in many formulations of tablets, suspensions, gels, etc. The study explores the potential of plant mucilage as an excipient in pharmaceutical formulations. The biodegradable and biocompatible properties of this inexpensive excipient make it more favourable for the newer formulation development.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"46 - 57"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49563463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shammy Jindal, R. Awasthi, Dhananjay S. Singare, G. Kulkarni
Summary Introduction: Psoriasis is an inflammatory skin disease characterized by hyper-proliferation, abnormal epidermal keratinocytes and inflammatory infiltration. It affects approximately 4% of the population globally. Herbal extracts have better results with less toxic effects than the synthetic drugs in the treatment of psoriasis. Objective: Present study was aimed to access the anti-psoriatic effect of andrographolide extracted from Andrographis paniculate (A. paniculata). Method: We extracted, characterized, and screened the extracted andrographolide for anti-proliferative characteristics using cultured cell model of human HaCaT keratinocyte. Results: Andrographolide at 31.25 µg/mL (90 µM) demonstrated significant inhibitory effect on human HaCaT keratinocytes proliferation in cell culture. To our best knowledge, we reported the anti-proliferative potency of andrographolide extracted from A. paniculata for the first time. Conclusion: The results suggest that the andrographolide extracted from A. paniculata plant may have potential to be used in the management of psoriasis.
{"title":"Isolation, characterization and evaluation of anti-proliferative properties of andrographolide isolated from Andrographis paniculata on cultured HaCaT cells","authors":"Shammy Jindal, R. Awasthi, Dhananjay S. Singare, G. Kulkarni","doi":"10.2478/hepo-2021-0002","DOIUrl":"https://doi.org/10.2478/hepo-2021-0002","url":null,"abstract":"Summary Introduction: Psoriasis is an inflammatory skin disease characterized by hyper-proliferation, abnormal epidermal keratinocytes and inflammatory infiltration. It affects approximately 4% of the population globally. Herbal extracts have better results with less toxic effects than the synthetic drugs in the treatment of psoriasis. Objective: Present study was aimed to access the anti-psoriatic effect of andrographolide extracted from Andrographis paniculate (A. paniculata). Method: We extracted, characterized, and screened the extracted andrographolide for anti-proliferative characteristics using cultured cell model of human HaCaT keratinocyte. Results: Andrographolide at 31.25 µg/mL (90 µM) demonstrated significant inhibitory effect on human HaCaT keratinocytes proliferation in cell culture. To our best knowledge, we reported the anti-proliferative potency of andrographolide extracted from A. paniculata for the first time. Conclusion: The results suggest that the andrographolide extracted from A. paniculata plant may have potential to be used in the management of psoriasis.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"67 1","pages":"35 - 45"},"PeriodicalIF":0.0,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43167484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ö. Ertürk, Gülçin Aydin, M. C. Ayvaz, Ceren Başkan
Summary Introduction: In order to prevent increased bacterial resistance, it is important to use herbal medicines with less side effects. Objective: In this study, the chemical composition, antimicrobial, antioxidant activities and SEM images of Lavandula officinalis and Origanum onites oils obtained in two different ways were determined. Methods: The antimicrobial activity of the oils was determined against reference and multiple strains of foodborne and pathogenic bacteria. The each essential oil sample were analyzed by GC–MS. Antioxidant activities of the samples were examined through DPPH• and ABTS•+ radical scavenging and FRAP assays. Results: The results indicate that the oils of obtained from Origanum onites and Lavandula officinalis exhibited relatively strong antibacterial and antifungal activity. In addition, the antioxidant activities detected were remarkable. Morphological changes in bacterial cells treated with essential oil samples were demon- strated with SEM images. In addition, the chemical components of the oil samples obtained by both water steam and cold press were revealed by GC-MS analysis and compared. Conclusion: When all the obtained results are evaluated together; these plants could be suitable for using as antimicrobial and antioxidative agents in several industries such as food, cosmetic, etc.
{"title":"Composition, antioxidant and antimicrobial activities of cold-pressed and distilled essential oils of Origanum onites L. and Lavandula officinalis L. relationships evaluated by SEM microscopy","authors":"Ö. Ertürk, Gülçin Aydin, M. C. Ayvaz, Ceren Başkan","doi":"10.2478/hepo-2020-0025","DOIUrl":"https://doi.org/10.2478/hepo-2020-0025","url":null,"abstract":"Summary Introduction: In order to prevent increased bacterial resistance, it is important to use herbal medicines with less side effects. Objective: In this study, the chemical composition, antimicrobial, antioxidant activities and SEM images of Lavandula officinalis and Origanum onites oils obtained in two different ways were determined. Methods: The antimicrobial activity of the oils was determined against reference and multiple strains of foodborne and pathogenic bacteria. The each essential oil sample were analyzed by GC–MS. Antioxidant activities of the samples were examined through DPPH• and ABTS•+ radical scavenging and FRAP assays. Results: The results indicate that the oils of obtained from Origanum onites and Lavandula officinalis exhibited relatively strong antibacterial and antifungal activity. In addition, the antioxidant activities detected were remarkable. Morphological changes in bacterial cells treated with essential oil samples were demon- strated with SEM images. In addition, the chemical components of the oil samples obtained by both water steam and cold press were revealed by GC-MS analysis and compared. Conclusion: When all the obtained results are evaluated together; these plants could be suitable for using as antimicrobial and antioxidative agents in several industries such as food, cosmetic, etc.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"125 20","pages":"43 - 54"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41308707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Introduction: India has a rich diversity of ethnomedicinal plants where the preparations from plants are used in treatment of various infection and ailments. Objectives: The detailed survey was done to gather the information about potential preparation of ethno-medicinal plants by the local communities of the study area. Methods: Data on 54 ethnomedicinal plants were recorded with the help of 19 men and 18 women aged between 45 and 60. Direct observation, group discussion and semi structured interview were used to collect the ethnobotanical information of the study area. Results: Prevalent ethnomedicinal uses of the plants were used for treating diarrhoea, asthma, dysentery, and other human ailments. The most commonly plant parts gathered were leaves (28.78 %) followed by bark (19.69 %), root (12.12 %), flower (10.60 %), fruit (9.09 %), seed and whole plant (7.57 % each), stem (4.54 %). It was observed from the investigation that the ethnomedicinal plants used most frequently in the treatment of diarrhoea and dysentery (29 taxa) followed by other liver problems (7 taxa), healing cut and wounds, tooth problems, blood dysentery, piles, asthma, fractured bones, scorpion/insects bite (2 taxa), skin diseases, urinary disorder and headache, menstrual disorder, infertility, stone problems, cold and cough, muscle pain and swelling, memory enhancer, killing of liver worms in children, insects repellent, bronchial catarrh (1 taxa each). Conclusion: The folk knowledge and ethnomedicinal preparation recorded in the present study area can be implemented in future for pharmacological and biological assay which could be further lead to new drug development.
{"title":"Quantitative ethnomedicinal study of plants used to cure different ailments in Rajaji tiger reserve, Uttarakhand, India","authors":"Akash, M. Zakir","doi":"10.2478/hepo-2020-0022","DOIUrl":"https://doi.org/10.2478/hepo-2020-0022","url":null,"abstract":"Summary Introduction: India has a rich diversity of ethnomedicinal plants where the preparations from plants are used in treatment of various infection and ailments. Objectives: The detailed survey was done to gather the information about potential preparation of ethno-medicinal plants by the local communities of the study area. Methods: Data on 54 ethnomedicinal plants were recorded with the help of 19 men and 18 women aged between 45 and 60. Direct observation, group discussion and semi structured interview were used to collect the ethnobotanical information of the study area. Results: Prevalent ethnomedicinal uses of the plants were used for treating diarrhoea, asthma, dysentery, and other human ailments. The most commonly plant parts gathered were leaves (28.78 %) followed by bark (19.69 %), root (12.12 %), flower (10.60 %), fruit (9.09 %), seed and whole plant (7.57 % each), stem (4.54 %). It was observed from the investigation that the ethnomedicinal plants used most frequently in the treatment of diarrhoea and dysentery (29 taxa) followed by other liver problems (7 taxa), healing cut and wounds, tooth problems, blood dysentery, piles, asthma, fractured bones, scorpion/insects bite (2 taxa), skin diseases, urinary disorder and headache, menstrual disorder, infertility, stone problems, cold and cough, muscle pain and swelling, memory enhancer, killing of liver worms in children, insects repellent, bronchial catarrh (1 taxa each). Conclusion: The folk knowledge and ethnomedicinal preparation recorded in the present study area can be implemented in future for pharmacological and biological assay which could be further lead to new drug development.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"66 1","pages":"55 - 67"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41467678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Radácsi, K. Inotai, S. Sárosi, K. Hári, K. Seidler-Łożykowska, Sintayehu Musie, É. N. Zámboriné
Summary Introduction: Irrigation plays an important role in the cultivation of medicinal plants. There is a lack of information on intraspecific variability of reactions to the effect of drought. Objective: The aim of the current study was to test the effect of irrigation on four sweet basil (Ocimum basilicum L.) cultivars (‘Genovese’, ‘Kasia’, ‘Keskenylevelű’, ‘Wala’). Methods: In an open field experiment irrigated and non-irrigated treatments were set. Plants were harvested in full flowering stage. Before the harvest, the stomatal conductance and chlorophyll content were measured. The production-related parameters were recorded. In the plant material the glandular hair density, essential oil content and composition (GC-MS) and volatile composition (SPME-GC-MS) were identified. Results: Stomatal conductance of the irrigated plants was 2.5–4.5 times higher than in the non-irrigated ones. The chlorophyll content showed only slight changes. The irrigation had a positive effect on the production. The leaf to stem ratio was not modified by the watering. Irrigation negatively influenced the glandular hair density and the EO content, however it increased the EO yield by 40–129%, depending on the cultivar. Significant compositional changes were registered only in the EO of cultivar ‘Kasia’ for linalool, 1,8-cineole and tau-cadinole ratios. Conclusion: Irrigation increases the biomass and EO yield of basil while the composition of the EO is mostly unchanged. Only slight differences were observed among the cultivars.
{"title":"Effect of irrigation on the production and volatile compounds of sweet basil cultivars (Ocimum basilicum L.)","authors":"P. Radácsi, K. Inotai, S. Sárosi, K. Hári, K. Seidler-Łożykowska, Sintayehu Musie, É. N. Zámboriné","doi":"10.2478/hepo-2020-0021","DOIUrl":"https://doi.org/10.2478/hepo-2020-0021","url":null,"abstract":"Summary Introduction: Irrigation plays an important role in the cultivation of medicinal plants. There is a lack of information on intraspecific variability of reactions to the effect of drought. Objective: The aim of the current study was to test the effect of irrigation on four sweet basil (Ocimum basilicum L.) cultivars (‘Genovese’, ‘Kasia’, ‘Keskenylevelű’, ‘Wala’). Methods: In an open field experiment irrigated and non-irrigated treatments were set. Plants were harvested in full flowering stage. Before the harvest, the stomatal conductance and chlorophyll content were measured. The production-related parameters were recorded. In the plant material the glandular hair density, essential oil content and composition (GC-MS) and volatile composition (SPME-GC-MS) were identified. Results: Stomatal conductance of the irrigated plants was 2.5–4.5 times higher than in the non-irrigated ones. The chlorophyll content showed only slight changes. The irrigation had a positive effect on the production. The leaf to stem ratio was not modified by the watering. Irrigation negatively influenced the glandular hair density and the EO content, however it increased the EO yield by 40–129%, depending on the cultivar. Significant compositional changes were registered only in the EO of cultivar ‘Kasia’ for linalool, 1,8-cineole and tau-cadinole ratios. Conclusion: Irrigation increases the biomass and EO yield of basil while the composition of the EO is mostly unchanged. Only slight differences were observed among the cultivars.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"66 1","pages":"14 - 24"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45246140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Introduction: The leaves of Lippia dulcis contain high amounts of hernandulcin. It is one thousand fold sweeter than sucrose, however, it hardly contains any calories. Objective: The aim of this research was to optimalisation of micropropagation and acclimatization of L dulcis Methods: The nodal explants were inoculated on phytohormone-free MS medium. After 6 weeks the explants were inoculated onto the MS medium with different plant growth regulators. Well-developed rooted plantlets were adapted to ex vitro conditions using hydrogel. Results: On the medium with BAP and NAA the highest number of shoots were produced. The higest average shoot length, number of the leaves and the leaf area were recorded on the medium with GA3. Adding IBA increased the number of roots. The addition of hydrogel enhanced the acclimatization efficiency. Conclusions: There was observed a positive, stimulating influence of growth regulators on mass propagation and increase in the number of leaves and the leaf area and influence of hydrogel on the development of plantlets during acclimatization.
{"title":"In vitro clonal propagation and efficient acclimatization with use of hydrogel of intensively sweet medicinal plant Lippia dulcis Trev.","authors":"M. Tomaszewska-Sowa","doi":"10.2478/hepo-2020-0019","DOIUrl":"https://doi.org/10.2478/hepo-2020-0019","url":null,"abstract":"Summary Introduction: The leaves of Lippia dulcis contain high amounts of hernandulcin. It is one thousand fold sweeter than sucrose, however, it hardly contains any calories. Objective: The aim of this research was to optimalisation of micropropagation and acclimatization of L dulcis Methods: The nodal explants were inoculated on phytohormone-free MS medium. After 6 weeks the explants were inoculated onto the MS medium with different plant growth regulators. Well-developed rooted plantlets were adapted to ex vitro conditions using hydrogel. Results: On the medium with BAP and NAA the highest number of shoots were produced. The higest average shoot length, number of the leaves and the leaf area were recorded on the medium with GA3. Adding IBA increased the number of roots. The addition of hydrogel enhanced the acclimatization efficiency. Conclusions: There was observed a positive, stimulating influence of growth regulators on mass propagation and increase in the number of leaves and the leaf area and influence of hydrogel on the development of plantlets during acclimatization.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"66 1","pages":"25 - 31"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42902913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Plants have been used to prepare herbal infusions for centuries. Production of herbal tea consists of several steps, beginning with harvesting, cleaning form residues, drying, storage of herb in a suitable material, grinding, and blending. Te plants grow in different regions and climatic conditions, varying by their physical properties. They are consumed for different purposes and due to their chemical constituents. Many of them have therapeutic effects. Besides their various benefits and even antimicrobial effects, they also carry some microorganisms. Thus, the botanical characteristics and effects on the health of frequently consumed herbal teas and recommendations on their consumption with considered microbial risks are reviewed in this article.
{"title":"Production stages, microbiological risk and benefits on health of herbal teas","authors":"G. Akduman, I. Korkmaz","doi":"10.2478/hepo-2020-0020","DOIUrl":"https://doi.org/10.2478/hepo-2020-0020","url":null,"abstract":"Summary Plants have been used to prepare herbal infusions for centuries. Production of herbal tea consists of several steps, beginning with harvesting, cleaning form residues, drying, storage of herb in a suitable material, grinding, and blending. Te plants grow in different regions and climatic conditions, varying by their physical properties. They are consumed for different purposes and due to their chemical constituents. Many of them have therapeutic effects. Besides their various benefits and even antimicrobial effects, they also carry some microorganisms. Thus, the botanical characteristics and effects on the health of frequently consumed herbal teas and recommendations on their consumption with considered microbial risks are reviewed in this article.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"66 1","pages":"68 - 78"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45839913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Summary Introduction:Angelica archangelica L. (Apiaceae) has a long history of use as a vegetable and medicinal plant. According to the European Pharmacopoeia, the angelica root (Angelica radix) of only one of the sub-species – Angelica archangelica subsp. archangelica (formerly known as Archangelica officinalis) – is used as a source of plant material with documented medicinal properties. Within this species, there are two subspecies that are difficult to classify unambiguously: subsp. archangelica and subsp. litoralis. Objective: The aim of this study was to provide a micromorphological and anatomical description of fruits of A archangelica and identify new diagnostic characters useful in subspecies identification. Methods: A comparative analysis of the sculpture and internal structure of fruits of the distinguished A archangelica taxa was conducted, using scanning electron microscopy (SEM). Results: Based on the taxonomic characters in the Apiaceae family, micromorphological and anatomical characteristics of A archangelica fruits were prepared. Some of the investigated characters, e.g. verrucose sculpture of the oil duct surface and the presence of hooked hairs, exhibited intraspecific differences. Among the anatomical characters, the variation was related to the number and size of the paraendocarpic oil ducts as well as to the thickness of dorsal and commissural mesocarp. Conclusion: SEM examination of fruits of A archangelica provided a detailed description of their sculptures and several micromorphological and anatomical characters of potential diagnostic value.
{"title":"Micromorphology and anatomy of fruits of Angelica archangelica L. (Apiaceae) and their intraspecific differentiation","authors":"A. Forycka, M. Morozowska","doi":"10.2478/hepo-2020-0018","DOIUrl":"https://doi.org/10.2478/hepo-2020-0018","url":null,"abstract":"Summary Introduction:Angelica archangelica L. (Apiaceae) has a long history of use as a vegetable and medicinal plant. According to the European Pharmacopoeia, the angelica root (Angelica radix) of only one of the sub-species – Angelica archangelica subsp. archangelica (formerly known as Archangelica officinalis) – is used as a source of plant material with documented medicinal properties. Within this species, there are two subspecies that are difficult to classify unambiguously: subsp. archangelica and subsp. litoralis. Objective: The aim of this study was to provide a micromorphological and anatomical description of fruits of A archangelica and identify new diagnostic characters useful in subspecies identification. Methods: A comparative analysis of the sculpture and internal structure of fruits of the distinguished A archangelica taxa was conducted, using scanning electron microscopy (SEM). Results: Based on the taxonomic characters in the Apiaceae family, micromorphological and anatomical characteristics of A archangelica fruits were prepared. Some of the investigated characters, e.g. verrucose sculpture of the oil duct surface and the presence of hooked hairs, exhibited intraspecific differences. Among the anatomical characters, the variation was related to the number and size of the paraendocarpic oil ducts as well as to the thickness of dorsal and commissural mesocarp. Conclusion: SEM examination of fruits of A archangelica provided a detailed description of their sculptures and several micromorphological and anatomical characters of potential diagnostic value.","PeriodicalId":12990,"journal":{"name":"Herba Polonica","volume":"66 1","pages":"1 - 13"},"PeriodicalIF":0.0,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46836954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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