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Analysis of actual nutrition of schoolchildren at different age periods 分析不同年龄段学童的实际营养状况
Pub Date : 2024-05-08 DOI: 10.47470/0016-9900-2024-103-4-342-348
N. V. Tapeshkina, T. D. Logunova, T. G. Korsakova, D. V. Pestereva
Introduction. A healthy, balanced diet can provide the child with nutritional protection from adverse environmental factors and helps to prevent diseases. Lack of continuity in nutrition at school and at home creates a deficit in the diet supply of nutrients necessary for a growing body. The purpose of the investigation is to study the actual nutrition of a group of schoolchildren at different age periods (in the dynamics of 10 years). Materials and methods. Actual nutrition of a group of schoolchildren in the dynamics over 2009 and 2019. The sample consisted of one hundred fifty 7-years children and the same children aged 17 years (124 children). Actual nutrition was studied using menu-layouts (at school) and the method of 24-hour (daily) nutrition reproduction (at home). Results. With age, there is a more pronounced imbalance in the supply of basic nutrients in the diet, associated with excess consumption of fats (7–25%), lack of carbohydrates, deficiency of dietary fibers (15–20%), a number of minerals (calcium, magnesium, iron) and vitamins (C, B1, B2, A, PP). Average daily food rations are unbalanced. The structure of children’s food sets becomes more irrational with increasing age. Regardless of the period of the study, diets are deficient in the level of consumption of milk, fish, vegetables, and fruits, and excessive in the level of consumption of foods with high energy value. In dynamics, the percentage of children with the recommended level of consumption of poultry is decreasing by 4.3 times, fish – by 3.8 times, milk – by 3 times, fermented milk products – by 2.6 times, cottage cheese and sour cream – by 2 times, butter and vegetable – 15.4 and 6.8 times, respectively. Limitations. This study is limited to a sample of a group of schoolchildren studying from the 1st to the 11th grade (in the dynamics with a ten-year interval). Conclusion. Persistent long-term violations of the basics of a healthy balanced diet by children create a probable risk of developing diseases in children in the future caused by nutritional factors, and can also be predictors of the development of pathological conditions and diseases in adulthood.
导言。健康、均衡的饮食可以为儿童提供营养保护,使其免受不利环境因素的影响,并有助于预防疾病。学校和家庭营养缺乏连续性,会造成膳食中人体生长所需的营养素供应不足。调查的目的是研究一组学龄儿童在不同年龄段(10 岁动态)的实际营养状况。材料和方法一组学龄儿童在 2009 年和 2019 年动态期间的实际营养状况。样本包括 150 名 7 岁儿童和 17 岁儿童(124 名)。实际营养状况的研究采用菜单布局(在学校)和 24 小时(每天)营养再现方法(在家里)。结果显示随着年龄的增长,膳食中基本营养素的供应失衡现象更加明显,这与脂肪摄入过多(7%-25%)、碳水化合物缺乏、膳食纤维不足(15%-20%)、多种矿物质(钙、镁、铁)和维生素(C、B1、B2、A、PP)有关。日均口粮不均衡。随着年龄的增长,儿童的食物结构越来越不合理。无论研究时间长短,膳食中牛奶、鱼类、蔬菜和水果的摄入量都不足,而高能量食品的摄入量过多。从动态上看,符合家禽建议食用量的儿童比例减少了 4.3 倍,鱼类减少了 3.8 倍,牛奶减少了 3 倍,发酵奶制品减少了 2.6 倍,松软干酪和酸奶油减少了 2 倍,黄油和蔬菜分别减少了 15.4 倍和 6.8 倍。局限性。本研究仅限于抽样调查 1 至 11 年级的在校学生(动态中间隔 10 年)。结论。儿童长期违反健康均衡饮食的基本原则,很可能导致儿童将来因营养因素而罹患疾病,也可能是成年后出现病理状况和疾病的预兆。
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引用次数: 0
Trend in indices of public health risk caused by air pollution in an industrial center 工业中心空气污染造成的公共健康风险指数趋势
Pub Date : 2024-05-08 DOI: 10.47470/0016-9900-2024-103-4-358-364
V. Kislitsyna, D. Surzhikov, Yu. S. Likontseva, V. A. Shtaiger
Introduction. In industrialized regions and cities, atmospheric air pollution greatly contributes to the hazard for public health. The study aim was to assess the state of the atmospheric air in the city of Novokuznetsk over 2017–2022 and determine the levels of risks to public health. Materials and methods. Data on gross atmospheric emissions and average annual concentrations of the substances was taken from the reports on the state of the environment of the Kemerovo region and Novokuznetsk. Maximum permissible concentrations of atmospheric pollutants were determined according to SanPiN 1.2.3685–21. Calculations of the risks to public health were carried out in accordance with Guideline P 2.1.10.1920–04. The classification of risk levels was carried out on the basis of MR 2.1.10.0156–19. 2.1.10. Results. Decrease in total atmospheric emissions from 2017 to 2022 amounted to 50.1 thousand tons (about 16%). At the same time, there was revealed an excess of the average annual maximum permissible concentrations of benzo(a)pyrene (3.5-11.2 times); formaldehyde (1.7 and 3.4 times over 2021 and 2022, respectively); suspended substances (1.6 and 2.0 times in 2021 and 2022, respectively); hydrogen fluoride (1.2 times in 2021); nitrogen dioxide (1.6 times in 2022). The hazard coefficients of benzo(a)pyrene were 3.5–11.2 (high level of non-carcinogenic risk); formaldehyde and suspended substances – 1.1–3.0 (alarming level). In 2022, the hazard coefficient for formaldehyde was 3.4 (high risk level), suspended substances – 2.0, nitrogen dioxide – 1.6 (alarming risk level). Hazard indices of pollutants were detected in the range from 7.0 to 20.1 (high level of risk). The main critical organs and systems were the immune system (hazard indices were 4.0–14.6), development during childhood (hazard indices – 3.5–11.2), respiratory organs (hazard indices – 3.0–8.6). These risk levels range from alarming to high. The highest total individual carcinogenic risk (1.6•10–4) was determined in 2021 (alarming level), the main contribution is made by formaldehyde. Limitations. In 2022, there were no data on carbon (soot) concentrations, so the level of total carcinogenic risk this year cannot be considered to be correct. Conclusion. Reducing the total volume of atmospheric emissions does not provide a significant effect on improving the environmental situation and public health, since high concentrations of pollutants remain in the city’s atmosphere. When implementing air protection measures, it is necessary to take into account risk criteria.
导言。在工业化地区和城市,大气空气污染极大地危害着公众健康。本研究旨在评估新库兹涅茨克市 2017-2022 年的大气空气质量状况,并确定其对公众健康的危害程度。材料和方法有关大气排放总量和物质年均浓度的数据来自克麦罗沃州和新库兹涅茨克市的环境状况报告。大气污染物的最高允许浓度是根据 SanPiN 1.2.3685-21 确定的。公众健康风险的计算是根据 P 2.1.10.1920-04 号准则进行的。风险等级的划分依据 MR 2.1.10.0156-19 进行。2.1.10.结果。从 2017 年到 2022 年,大气排放总量减少了 5.01 万吨(约 16%)。与此同时,苯并(a)芘(3.5-11.2 倍)、甲醛(2021 年和 2022 年分别为 1.7 倍和 3.4 倍)、悬浮物质(2021 年和 2022 年分别为 1.6 倍和 2.0 倍)、氟化氢(2021 年为 1.2 倍)、二氧化氮(2022 年为 1.6 倍)的年均最大允许浓度出现超标。苯并(a)芘的危害系数为 3.5-11.2(非致癌风险水平高);甲醛和悬浮物质--1.1-3.0(警戒水平)。2022 年,甲醛的危害系数为 3.4(高风险水平),悬浮物质为 2.0,二氧化氮为 1.6(警戒风险水平)。检测到的污染物危害指数在 7.0 至 20.1 之间(高风险水平)。主要的关键器官和系统是免疫系统(危险指数为 4.0-14.6)、儿童发育(危险指数 - 3.5-11.2)、呼吸器官(危险指数 - 3.0-8.6)。这些风险水平从惊人到很高不等。2021 年的个人总致癌风险(1.6-10-4)最高(警戒水平),主要来自甲醛。局限性。2022 年没有关于碳(烟尘)浓度的数据,因此不能认为这一年的总致癌风险水平是正确的。结论。由于城市大气中仍存在高浓度污染物,因此减少大气排放总量对改善环境状况和公众健康的效果并不显著。在实施空气保护措施时,有必要考虑风险标准。
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引用次数: 0
The influence of the type of microcirculatory disorders on the development of trophic changes in the skin of the hands in patients with vibration disease 微循环障碍类型对振动病患者手部皮肤营养性变化发展的影响
Pub Date : 2024-05-08 DOI: 10.47470/0016-9900-2024-103-4-328-332
A. V. Yamshchikova, Nadezhda I. Shumeiko, A. Fleishman, I. Martynov, Tatiana A. Bychkovskaya
Introduction. Hypoxia caused by microcirculation disorders in patients with vibration disease leads to changes in the concentration and, accordingly, fluorescence of various biologically active substances-fluorophores in the skin of the hands, which are the markers of trophic disorders. Microcirculation disorders in this category of patients are shown to have a multidirectional character. The study of the effect of the type of microcirculatory changes on the development of trophic disorders in the skin of the hands is relevant for the patients with vibration disease. The aim of the study was to evaluate the accumulation of biologically active substances based on their fluorescence in the skin of the hands in patients with vibration disease depending on the type and degree of microcirculation disorder. Materials and methods. Thirty three miners with a proven diagnosis of vibration disease and 15 conventionally healthy men of close age without complaints and never exposed to industrial vibration were examined. Microcirculation was studied using laser Doppler flowmetry, the coefficient of fluorescent contrast of biological tissue was determined applying noninvasive optical tissue fluorescence spectroscopy. Results. The multidirectional types of microcirculation disorders in the extremities in vibration disease were revealed. Regardless of the direction of microcirculatory disorders, occurred hypoxia leads to the accumulation of biologically active substances - markers of trophic skin changes, as well those involved in antioxidant protection. A reliable gain in the fluorescence of these substances was noted in both hyperemic and spastic disorders, more significant in the hyperemic type. Limitations. The study was limited to assessing the microcirculation types and fluorescent contrast of biological tissues in 33 patients with vibration disease and in 15 conventionally healthy subjects who had never worked under the conditions of exposure to industrial vibration. Conclusion. Changes in the trophism of the skin of the hands in patients with vibration disease develop as a result of chronic hypoxia, which is the main pathophysiological link of angiodistonic syndrome, regardless of the direction of microcirculatory disorders, however, the hyperemic type can be conditionally considered more unfavourable with respect to the development of trophic skin disorders.
导读:振动病患者因微循环障碍而缺氧,导致手部皮肤中各种生物活性物质--荧光团的浓度发生变化,荧光团也相应发生变化。振动病患者微循环障碍引起的缺氧会导致手部皮肤中各种生物活性物质--荧光团--的浓度发生变化,荧光团是营养障碍的标志。这类患者的微循环障碍具有多向性。研究微循环变化的类型对手部皮肤营养障碍发展的影响对振动病患者具有重要意义。本研究的目的是根据微循环障碍的类型和程度,评估生物活性物质在振动病患者手部皮肤中的荧光积累情况。材料和方法研究对象包括 33 名确诊为振动病的矿工和 15 名年龄相仿、无任何不适症状且从未接触过工业振动的普通健康男性。使用激光多普勒血流测量仪对微循环进行了研究,并使用无创光学组织荧光光谱测定了生物组织的荧光对比系数。研究结果揭示了振动病肢体微循环障碍的多向类型。无论微循环障碍的方向如何,缺氧都会导致生物活性物质的积累--这些物质是皮肤营养变化的标志,也是抗氧化保护的标志。在充血型和痉挛型疾病中,这些物质的荧光都有可靠的增加,充血型更为显著。局限性。该研究仅限于评估 33 名振动疾病患者和 15 名从未在工业振动条件下工作过的常规健康受试者的微循环类型和生物组织的荧光对比度。研究结论无论微循环障碍的方向如何,振动病患者手部皮肤营养障碍的变化都是慢性缺氧的结果,而慢性缺氧是血管营养不良综合征的主要病理生理环节,不过,可以有条件地认为高充血型更不利于皮肤营养障碍的发展。
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引用次数: 0
Hygienic aspects of mortality of the population of an industrial city 工业城市人口死亡率的卫生问题
Pub Date : 2024-04-10 DOI: 10.47470/0016-9900-2024-103-3-227-233
E. Ovchinnikova, A. S. Kolchin, AS Kriga, O. Plotnikova, N. Shirinskaya
Introduction. The target indicators of the Federal Project “Clean Air” include a reduction in emissions of chemical pollutants by at least 20%. However, the main socially significant goal remains to improve the medical and demographic situation by reducing environmental and hygienic health risks. The purpose of the study was to preliminary assess the reserves for reducing mortality in the city of Omsk by identifying the most unfavourable territories in terms of atmospheric air quality and identify priority chemicals, presumably causing negative trend in mortality. Materials and methods. To assess the quality of atmospheric air in the city of Omsk, the results of social and hygienic monitoring, environmental monitoring of Federal Service of Russia on Hydrometeorology and Monitoring of the Environment (RosGidromet) for the multi-year period over 2009–2022 were used. An analysis of the mortality rate of the population of Omsk for 2017–2022 was carried out. The data were studied in the context of municipal districts of the city of Omsk and in dynamics. Statistical methods were used in the study: ANOVA, correlation analysis, assessment of relative mortality risks. Results. The relative risk (RR) of mortality for persons living in the most polluted areas of the city is 23–25% higher than in conditionally clean areas. Strong positive associations have been established: between the levels of actual total mortality and the specific gravity of atmospheric air samples exceeding the maximum single limit concentrations (R = 0.86; p < 0.05); between perennial average annual carbon monoxide concentrations and standardized rates of total mortality (R = 0.93; p<0.05) and male mortality rates from all natural causes (R = 0.89; p<0.05). Limitations. The study is limited to the statistical research methods used. Conclusion. The results of the study can become the basis for more efficient use of administrative resources in municipal districts of the city. As additional reserves for reducing mortality, the development of primary prevention measures at the outpatient level is important. It is necessary to optimize methodological approaches in the health control system of the population living in risk areas with developed industry.
导言。清洁空气 "联邦项目的目标指标包括将化学污染物的排放量至少减少 20%。然而,具有社会意义的主要目标仍然是通过减少环境和卫生健康风险来改善医疗和人口状况。研究的目的是通过确定鄂木斯克市大气空气质量最差的地区和确定可能导致死亡率下降趋势的重点化学物质,初步评估鄂木斯克市降低死亡率的储备。材料和方法为了评估鄂木斯克市的大气质量,使用了社会和卫生监测结果,以及俄罗斯水文气象和环境监测联邦局(RosGidromet)2009-2022 年多年期的环境监测结果。对 2017-2022 年鄂木斯克人口死亡率进行了分析。数据是在鄂木斯克市辖区范围内和动态范围内研究的。研究使用了统计方法:方差分析、相关分析、相对死亡风险评估。研究结果。生活在城市污染最严重地区的人的相对死亡风险(RR)比生活在有条件清洁地区的人高 23-25%。实际总死亡率与超过最高单一限值浓度的大气空气样本比重之间(R = 0.86;P < 0.05);一氧化碳常年平均年浓度与总死亡率标准化率(R = 0.93;P < 0.05)和所有自然原因导致的男性死亡率(R = 0.89;P < 0.05)之间建立了很强的正相关关系。局限性。该研究仅限于所使用的统计研究方法。结论。研究结果可作为该市各区更有效利用行政资源的依据。作为降低死亡率的额外储备,制定门诊一级的初级预防措施非常重要。有必要优化工业发达的风险地区居民健康控制系统的方法。
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引用次数: 0
Comprehensive hygienic assessment of ambient air pollution in the area of food and processing industry enterprises’ location 食品和加工业企业所在地环境空气污染综合卫生评估
Pub Date : 2024-04-10 DOI: 10.47470/0016-9900-2024-103-3-198-207
Olga A. Budarina, Zulfiya F. Sabirova, Svetlana V. Skovronskaya, N. S. Dodina, Aleksandra A. Kokhan, Alla G. Malysheva
The purpose of the study is to assess atmospheric air pollution in a city where food and processing enterprises operate using a complex of instrumental, olfacto-odourimetric, calculated, and survey methods. Materials and methods. Analytical studies of ambient air pollution were performed using the FOCUS GC-DSQ-II chromatography-mass spectrometer. Olfacto-odourimetric studies of the presence and intensity of odour in the area of the enterprises were carried out in accordance with the method of organoleptic control downwind at various distances from sources. The measurement of the odour level at the discharge of the coffee extract spray dryer was carried out by dynamic olfactometry on the ECOMA TO-8 olfactometer. The calculation of odour dispersion was implemented according to a program using a methodology for calculating concentrations of harmful substances in ambient air. Results. It was established that at the time of the research, the source of a persistent specific odour reaching the residential area was a coffee production enterprise. Up to seventy eight volatile organic compounds in concentrations not exceeding hygienic standards have been identified in the ambient air in the area of the enterprise’s location. Field olfacto-odorimetric studies and the conducted dispersion calculation indicate an offensive odour to be capable of causing “annoyance” to the population (3 points or higher with a probability of ≥ 5% according to the results of field studies or ≥ 3.5 OU (odour units) according to the calculation results) spreads up to 4 km from the main source of emissions. Limitations. The main limitation of the study is the peculiarities of the operating mode of different enterprises, which makes it difficult to fully assess the environmental situation in the area of their location at the time of the research. Conclusion. The results of these studies indicate the use of various methodological approaches (field olfacto-odorimetric measurements, calculation of odour dispersion), along with conducting population surveys, to significantly expand the possibilities of instrumental analysis for an objective assessment of ambient air pollution by complex multicomponent mixtures of odorous substances.
本研究的目的是采用综合仪器法、嗅觉气味测定法、计算法和调查法,评估食品加工企业所在城市的大气空气污染情况。材料和方法使用 FOCUS GC-DSQ-II 色谱-质谱仪对环境空气污染进行分析研究。根据感官控制方法,在距离污染源不同距离的下风处对企业所在区域的气味存在情况和强度进行了嗅觉-味觉测量研究。对咖啡提取物喷雾干燥机排放口气味水平的测量是通过 ECOMA TO-8 嗅觉测量仪进行的动态嗅觉测量。气味扩散的计算是通过一个使用环境空气中有害物质浓度计算方法的程序进行的。计算结果研究结果表明,在研究期间,到达居民区的持久性特殊气味的来源是一家咖啡生产企业。在该企业所在地的环境空气中发现了多达 78 种挥发性有机化合物,其浓度未超过卫生标准。现场嗅觉-气味测定研究和进行的扩散计算表明,难闻的气味能够对居民造成 "烦扰"(根据现场研究结果,3 点或更高,概率≥ 5%;根据计算结果,≥ 3.5 OU(气味单位)),扩散到距离主要排放源 4 公里的地方。局限性。本研究的主要局限性在于不同企业运营模式的特殊性,因此很难全面评估研究时企业所在区域的环境状况。结论这些研究结果表明,使用各种方法(现场嗅觉气味测量、气味扩散计算),同时进行人口调查,可以大大提高仪器分析的可能性,从而客观评估由复杂的多组分气味物质混合物造成的环境空气污染。
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引用次数: 0
Occupational prevalence rates in metallurgists in the Sverdlovsk Region 斯维尔德洛夫斯克地区冶金工人的职业发病率
Pub Date : 2024-04-10 DOI: 10.47470/0016-9900-2024-103-3-253-257
V. Gazimova
Introduction. The previous analysis conducted for 2003–2022 showed ferrous industry employing over twenty five thousand people to make the most contribution to occupational disease incidence rates in the Sverdlovsk Region since 68.6 % of its workers are affected by industrial exposures above occupational safety standards. The purpose of the study was the analysis of occupational disease incidence in workers of a ferrous metal processing plant of the Sverdlovsk Region and assessment of the impact of adverse factors of the work environment and industrial processes on its rates. Materials and methods. Long-term occupational disease incidence in workers of the ferrous metal processing plant has been analyzed by the causative agent, nosology, age, gender, and job. Data of the reporting form No. 1-T (working conditions) for the study period have been scrutinized; comparative data evaluation by working conditions and occupational disease incidence rates has been made. Results. High incidence rates of work-related diseases in the metallurgists indicate to a significant proportion (70 %) of them to be exposed to occupational hazards. The established 20-year rate at the enterprise was 5.6 and 1.6 times higher than the regional and Russian averages in this industry, respectively. Increased gas and dust levels in the workplace air remain the leading health risk factors in ferrous metallurgy. Limitations. The limitation of this study was the lack of annual data on workers by job preventing calculation of job-specific disease incidence rates per 10 thousand workers. Conclusion. Despite the substantial modernization of the metallurgical complex, metal processing technology still does not comply with safety standards, thus requiring the development and implementation of science-based preventive measures aimed at maintaining workers’ health.
导言。先前进行的 2003-2022 年分析表明,在斯维尔德洛夫斯克地区,拥有超过 2.5 万名员工的黑色金属工 业对职业病发病率的影响最大,因为该行业 68.6%的工人受到高于职业安全标准的工业接触影响。本研究的目的是分析斯维尔德洛夫斯克地区一家黑色金属加工厂工人的职业病发病率,并评估工作环境和工业流程的不利因素对发病率的影响。材料和方法对黑色金属加工厂工人的长期职业病发病率按病原体、病名、年龄、性别和工作进行了分析。对研究期间第 1-T 号报告表(工作条件)的数据进行了仔细检查;并根据工作条件和职业病发病率进行了数据比较评估。研究结果冶金工人的职业病发病率很高,这表明他们中有很大一部分人(70%)受到职业危害。该企业 20 年的既定发病率分别是该地区和俄罗斯该行业平均发病率的 5.6 倍和 1.6 倍。工作场所空气中气体和粉尘含量的增加仍然是黑色冶金行业的主要健康风险因素。局限性。本研究的局限性在于缺乏按工种分列的工人年度数据,因此无法计算每万名工人中特定工种的疾病发病率。结论。尽管冶金综合体实现了大幅现代化,但金属加工技术仍不符合安全标准,因此需要制定和实施科学的预防措施,以维护工人的健康。
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引用次数: 0
Effect of chronic stress on the degree of acrylamide toxicity in rats 慢性应激对大鼠丙烯酰胺毒性程度的影响
Pub Date : 2024-04-10 DOI: 10.47470/0016-9900-2024-103-3-258-265
A.A. Gizatullina, N. Khusnutdinova, D. D. Karimov, D. A. Smolyankin, Yana V. Valova, D. Karimov, Guzel F. Muhammadieva, E. F. Repina, A. R. Akhmadeev
Introduction. Chronic stress plays an important role in the development of a wide range of mental, somatic and behavioural disorders, and therefore is a risk factor for the health in living organisms. A negative effect on the state of the nervous system is also observed when the body is poisoned with acrylamide, which is a substance of the second class of danger and is considered toxic and carcinogenic. Exposure of the body to mental and toxic stressors leads to autonomic and neuroendocrine activation, which, in turn, manifests itself as special behavioural patterns. The purpose of the experiment was to assess the effect of chronic stress on the degree of acrylamide toxicity in rats. Materials and methods. The experiment was conducted on white outbred rats with an average weight of 200 g of both sexes (n=60), which were evenly distributed into five groups: negative control, chronic stress, acrylamide, acrylamide + treatment, acrylamide + chronic stress. Throughout the study, the animals were kept under standard conditions with 12 hours of artificial lighting during the day, a relatively constant level of humidity (30–70%) and an air temperature of 20–25 °C. Behavioural tests were conducted once a week for one calendar month using a 40×40 hole board, a multifunctional cage to assess general activity, and an elevated plus maze with an ANY-maze video tracking system. Biochemical parameters were assessed in accordance with the manufacturer’s instructions. Results. Analysis of behaviour on a board with holes did not reveal statistically significant results (H=8.987; p=0.061). When comparing vertical and horizontal motor activity between groups, statistically significant differences were found (p<0.05). AST levels were higher in the stress groups, while cholesterol levels in the same groups were lower (p<0.05). Limitations. Laboratory animals of only one biological species were used for the experiment. The toxicant was used only in one concentration. Conclusion. Chronic stress may influence on acrylamide toxicity in rats to a certain extent.
导言慢性压力在各种精神、躯体和行为失调的发展过程中起着重要作用,因此是影响生物体健康的一个危险因素。当人体受到丙烯酰胺的毒害时,神经系统的状态也会受到负面影响,丙烯酰胺属于第二类危险物质,被认为具有毒性和致癌性。人体暴露在精神和有毒压力下会导致自律神经和神经内分泌的激活,进而表现为特殊的行为模式。实验的目的是评估慢性压力对大鼠丙烯酰胺毒性程度的影响。材料和方法实验对象是平均体重为 200 克的白色纯种大鼠,雌雄均有(n=60),平均分为五组:阴性对照组、慢性应激组、丙烯酰胺组、丙烯酰胺 + 治疗组、丙烯酰胺 + 慢性应激组。在整个研究过程中,动物均在标准条件下饲养,白天人工照明 12 小时,湿度相对恒定(30-70%),气温 20-25 °C。在一个日历月内,每周进行一次行为测试,使用 40×40 孔板、多功能笼子评估一般活动,并使用 ANY-maze 视频跟踪系统进行高架加迷宫测试。生化指标按照制造商的说明进行评估。结果在带孔的木板上的行为分析结果没有统计学意义(H=8.987;P=0.061)。在比较各组之间的垂直和水平运动活动时,发现差异有统计学意义(p<0.05)。压力组的谷草转氨酶水平较高,而同组的胆固醇水平较低(P<0.05)。局限性。实验只使用了一种生物物种的实验动物。仅使用了一种浓度的毒物。结论。慢性应激可能会在一定程度上影响丙烯酰胺对大鼠的毒性。
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引用次数: 0
Soil pollution of the city with machine-building production by heavy metals and arsenic and epidemiological risk to public health 重金属和砷对机械制造城市的土壤污染以及对公众健康的流行病学风险
Pub Date : 2024-04-10 DOI: 10.47470/0016-9900-2024-103-3-208-215
A. Martsev, O. Selivanov, Yuri N. Kurbatov, T. Trifonova
Introduction. The paper presents the results of a study for the assessment of the epidemiological risk to public health and the level of soil contamination with heavy metals and arsenic in the city of Kovrov, a large industrial center of the Vladimir region. Materials and methods. The objects of the study are the morbidity in the population of the Kovrovsky district of the Vladimir region and the soil cover of the city of Kovrov, whose industrial enterprises specialize in the production of machine-building products. Soil samples were taken in the area of industrial enterprises, highways, and residential areas. The soil cover was examined by X-ray fluorescence method to determine the content of heavy metals and arsenic. Results. The conducted study on the assessment of probable risk allowed establishing in the Kovrov district, relative to background regional values, children to have a very high risk for 10 classes of diseases, adults have a very high risk for 6 classes of diseases. Contamination of the Carpet city soil with heavy metals and arsenic has been established. The priority pollutants of the Kovrov soil are zinc, lead and arsenic, which belong to the first hazard class. There was a significant variation in the content of heavy metals within both industrial and residential zones. Extremely dangerous chemical contamination of the soil with heavy metals has been detected. The assessment of the level of chemical soil pollution, as an indicator of adverse effects on public health, showed the residential part of the city, where most of the residents live, to be located in the zone of moderately dangerous or dangerous category of soil pollution. Limitations. The limitations of the study are related to one-time sampling and a small number of reference sites, which limits the possibilities of a broader interpretation of the data obtained. Conclusion. The unfavourable state of the soil cover in the residential area of Kovrov requires the manaagement and conducting constant ecological and hygienic monitoring of this territory. To reduce pollutants in particular, heavy metals, entering the soil of the city and, it is necessary to improve the system for cleaning gas-air emissions of machine-building enterprises of the city, as well as to ensure the effective arrangement of sanitary protection zones. To reduce the content of heavy metals in the soil, it is necessary to carry out measures to detoxify contaminated soils.
导言。本文介绍了对弗拉基米尔地区大型工业中心科夫罗夫市公众健康的流行病学风险以及土壤重金属和砷污染水平进行评估的研究结果。材料和方法。研究对象是弗拉基米尔州科夫罗夫斯基区人口的发病率和科夫罗夫市的土壤覆盖率,该市的工业企业专门生产机械制造产品。在工业企业、高速公路和居民区采集了土壤样本。土壤覆盖层采用 X 射线荧光法进行检测,以确定重金属和砷的含量。研究结果通过对可能风险的评估研究,可以确定在科夫罗夫区,相对于地区背景值,儿童罹患 10 种疾病的风险非常高,成人罹患 6 种疾病的风险非常高。地毯城市土壤重金属和砷污染已经确定。科夫罗夫土壤中的重点污染物是锌、铅和砷,它们属于第一危险等级。工业区和居民区的重金属含量差异很大。已发现土壤中存在极其危险的重金属化学污染。作为对公众健康产生不利影响的指标,对土壤化学污染水平的评估显示,该市大部分居民居住的居民区位于中度危险或危险土壤污染区域。局限性。研究的局限性在于一次性采样和参考点数量较少,这限制了对所获数据进行更广泛解释的可能性。结论。科夫罗夫居民区的土壤覆盖状况不佳,需要对这一地区进行管理,并持续进行生态和卫生监测。为了减少进入城市土壤的污染物,特别是重金属,有必要改进城市机械制造企业的废气净化系统,并确保有效安排卫生保护区。为了减少土壤中的重金属含量,有必要采取措施对受污染的土壤进行解毒。
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引用次数: 0
Determination of antibiotics of the tetracycline group in water by high-performance liquid chromatography on a diode matrix detector with preliminary concentration by solid-phase extraction 采用二极管矩阵检测器上的高效液相色谱法和固相萃取初步浓缩法测定水中的四环素类抗生素
Pub Date : 2024-04-10 DOI: 10.47470/0016-9900-2024-103-3-266-272
Larisa P. Nekrasova, Oksana Ju. Kuleshova
Introduction. Antibiotic contamination of the environment is a serious environmental threat that poses a hazard to human health. To monitor the content of tetracycline antibiotics in environmental objects and control technological processes aimed at their disposal, accessible analytical methods are needed. Purpose of the study. Development of a method for determining antibiotics of the tetracycline group in water using a diode array detector with preliminary solid-phase concentration. Material and methods. The objects of the study were model solutions of minocycline, tetracycline, oxytetracycline, demeclocycline, metacycline, and doxycycline in deionized, tap, natural, and treated wastewater. For solid-phase extraction, Diapak P and Diapak PG cartridges were used. SPE was performed using a VacMaster-10 manifold (Biotage). Chromatographic separation was carried out on Diasphere C10CN and Kromasil Eternity 250 × 4.6 mm 5 µm columns on an Agilent 1100 liquid chromatograph (Agilent Technology). Results. Optimal conditions for the chromatographic separation of minocycline, tetracycline, oxytetracycline, demeclocycline, metacycline, and doxycycline were selected: isocratic mode, wavelength of 350 nm, mobile phase – acetonitrile: aqueous solution of phosphoric acid (pH = 3.0). The analysis time on Diasphere C10CN and Kromasil Eternity columns was 12 and 14 minutes, respectively. The reliability of the linear approximation in both cases was more than 0.99, however, the slopes on the Kromasil Eternity column were 1.35 –1.65 times higher than on Diasphere C10CN. The degree of extraction of tetracyclines from deionized water on Diapak P and Diapak PG cartridges was 90–95%, from tap water 61–89%, from purified waste water: 51–87%. Limitations. The method is not suitable for water bodies with tetracycline contents less than 2 µg/dm3. Conclusion. An HPLC method has been developed for the determination of minocycline, tetracycline, oxytetracycline, demeclocycline, metacycline, and doxycycline in water with preliminary SPE concentration on Diapak P and Diapak PG cartridges. The lower limit of determination for the sorption of target compounds from 0,1 dm3 of sample was 2 μg/dm3.
导言。环境中的抗生素污染是一种严重的环境威胁,对人类健康造成危害。为了监测环境物体中四环素类抗生素的含量,并控制处理这些抗生素的技术过程,需要有简便易行的分析方法。研究目的开发一种利用二极管阵列检测器初步固相浓缩法测定水中四环素类抗生素含量的方法。材料和方法。研究对象是去离子水、自来水、天然废水和处理过的废水中的米诺环素、四环素、土霉素、地美环素、甲氧环素和强力霉素的模型溶液。固相萃取使用的是 Diapak P 和 Diapak PG 滤芯。固相萃取使用的是 VacMaster-10 歧管(Biotage)。色谱分离采用安捷伦 1100 型液相色谱仪(安捷伦科技公司)上的 Diasphere C10CN 和 Kromasil Eternity 250 × 4.6 mm 5 µm 色谱柱。结果选择了米诺环素、四环素、土霉素、地美环素、甲氧环素和强力霉素色谱分离的最佳条件:等度模式,波长 350 nm,流动相 - 乙腈:磷酸水溶液(pH = 3.0)。Diasphere C10CN 和 Kromasil Eternity 色谱柱的分析时间分别为 12 分钟和 14 分钟。两种色谱柱的线性近似可靠性均大于 0.99,但 Kromasil Eternity 色谱柱的斜率是 Diasphere C10CN 色谱柱的 1.35-1.65 倍。Diapak P 和 Diapak PG 滤芯对去离子水中四环素的萃取率为 90-95%,对自来水的萃取率为 61-89%,对净化废水的萃取率为 51-87%:51-87%.局限性。该方法不适用于四环素含量低于 2 µg/dm3 的水体。结论。通过在 Diapak P 和 Diapak PG 色谱柱上进行初步固相萃取浓缩,建立了测定水中米诺环素、四环素、土霉素、地美环素、甲氧环素和强力霉素的高效液相色谱法。0.1 dm3 样品中目标化合物的吸附测定下限为 2 μg/dm3。
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引用次数: 0
Machine learning for public wellness: optimizing hygiene practices and pollution monitoring in smart cities 机器学习促进公共卫生:优化智慧城市的卫生习惯和污染监测
Pub Date : 2024-04-10 DOI: 10.47470/0016-9900-2024-103-3-216-222
Ramanathan Udayakumar
Introduction. Public health in urban areas is of paramount importance, particularly in the context of smart cities where technology plays a vital role. The integration of sophisticated infrastructure and data-driven systems in smart cities has the potential to significantly enhance public health outcomes. This improvement hinges on optimizing various factors, especially in the realms of hygiene standards and pollution monitoring. The ability to adhere to stringent hygiene procedures and closely monitor pollutants is essential for mitigating health risks in densely populated urban environments. As metropolitan areas become increasingly complex, there is a pressing need to prioritize the optimization of these processes. Materials and Methods. To address the challenges associated with public health optimization in smart cities, this study introduces Optimized Public Wellness using Machine Learning (OPWML). OPWML employs advanced machine learning techniques to augment hygiene protocols and pollution surveillance in smart urban areas. The proposed approach incorporates real-time validation, enhanced data-collecting efficiency, intelligent intervention impact, and increased throughput. The methodology aims to streamline processes and overcome the limitations of current approaches, providing more precise and prompt outcomes. Results. Simulation findings demonstrate the superior performance of OPWML compared to other methods. The average estimate accuracy achieved by OPWML is 86.76%, showcasing its efficacy in delivering accurate results. Real-time validation latency is notably low at 12.99 ms, indicating the system’s responsiveness. With a data collection efficiency of 22.96 GB/hour, OPWML demonstrates its ability to efficiently gather relevant data. The smart intervention impact of 33.20% underscores the system’s effectiveness in implementing intelligent interventions. Additionally, the throughput of 314.67 kbps signifies the high processing capacity of OPWML. Limitations. While OPWML exhibits promising results, it is essential to acknowledge certain limitations in this study. The simulation-based nature of the findings may not fully capture real-world complexities. Additionally, the generalizability of the results to diverse urban contexts requires further investigation. Limitations such as data privacy concerns and potential technological barriers should also be considered when implementing OPWML in practical settings. Conclusion. In conclusion, Optimized Public Wellness using Machine Learning (OPWML) emerges as a powerful tool for transforming public health processes in smart cities. The study highlights OPWML’s capacity to significantly enhance hygiene protocols and pollution surveillance, ensuring a healthier and environmentally sustainable urban setting. While acknowledging certain study limitations, the overall outcomes emphasize the potential of OPWML in revolutionizing public health practices and contributing to the well-being of urban pop
导言。城市地区的公共卫生至关重要,尤其是在智能城市的背景下,技术发挥着至关重要的作用。在智慧城市中整合先进的基础设施和数据驱动系统,有可能显著提高公共卫生成果。这种改善取决于各种因素的优化,特别是在卫生标准和污染监测方面。在人口稠密的城市环境中,遵守严格的卫生程序和密切监测污染物的能力对于降低健康风险至关重要。随着大都市地区变得日益复杂,迫切需要优先优化这些流程。材料与方法。为了应对与智慧城市公共卫生优化相关的挑战,本研究引入了机器学习优化公共卫生(OPWML)。OPWML 采用先进的机器学习技术来增强智能城市地区的卫生协议和污染监测。建议的方法包括实时验证、提高数据收集效率、智能干预影响和增加吞吐量。该方法旨在简化流程,克服当前方法的局限性,提供更精确、更迅速的结果。结果。模拟结果表明,与其他方法相比,OPWML 的性能更优越。OPWML 实现的平均估算准确率为 86.76%,显示了其在提供准确结果方面的功效。实时验证延迟时间明显较低,仅为 12.99 毫秒,显示了系统的响应速度。OPWML 的数据收集效率为 22.96 GB/小时,显示了其高效收集相关数据的能力。33.20% 的智能干预影响强调了系统在实施智能干预方面的有效性。此外,314.67 kbps 的吞吐量也证明了 OPWML 的高处理能力。局限性。虽然 OPWML 取得了可喜的成果,但必须承认本研究存在一定的局限性。研究结果的模拟性质可能无法完全反映现实世界的复杂性。此外,还需要进一步调查研究结果在不同城市环境中的通用性。在实际环境中实施 OPWML 时,还应考虑数据隐私问题和潜在的技术障碍等限制因素。结论总之,利用机器学习优化公共卫生(OPWML)是改变智慧城市公共卫生流程的有力工具。本研究强调了 OPWML 的能力,它能显著增强卫生协议和污染监测,确保城市环境更健康、环境更可持续。虽然研究存在一定的局限性,但总体成果强调了 OPWML 在革新公共卫生实践和促进智慧城市时代城市人口福祉方面的潜力。
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引用次数: 0
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Hygiene and sanitation
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