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Evaluation of certain pomological and phenological traits of selected asian pear varieties growing in Middle European conditions 在中欧条件下生长的若干亚洲梨品种的果学和物候特征评价
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-06-30 DOI: 10.17221/83/2019-hortsci
T. Nečas, J. Wolf, T. Kiss, Martina Gottingerová, Ondrášek Ivo, M. Bieniasz
The cultivation and breeding of new pear tree varieties have great potential, especially with the changing climate. The introduction of genetic resources and varieties from regions with a high genetic diversity can have a positive effect on the cultivation of pear trees in our region. In this experiment, a total of 23 Asian pear varieties originating from Japan and China were assessed. Based on the values obtained, the varieties were sorted, and suitable varieties for the conditions of the Czech Republic were selected. The early-maturing varieties that proved to be promising were ‘Shinseiki’ and ‘Zao Su Li’ due to their refreshing juiciness, plasticity and ease of cultivation. generally low demand. The ‘Shinseiki’ bears mediumto small-sized fruits (103 g on average). The size of the fruits of the evaluated cultivar ‘Zao Su Li’ can reach very large sizes (255 g on average). The medium-maturity varieties suitable for the conditions of the Czech Republic were ‘Hosui’ and ‘Dangshansu Li’ (ripening on the September 11–19 on average) and another interesting variety, ‘Kirgizkaja zimnaja’. Other suitable medium-maturity varieties are ‘Nijisseiki’, ‘Sha Li’ and ‘Pung Su’. Of the assessed late-maturing varieties, the promising ones were ‘Ya Li’ and ‘Mut Chen’, especially due to their relatively high storability under cold storage.
随着气候的变化,梨树新品种的培育和选育具有很大的潜力。从遗传多样性高的地区引进遗传资源和品种,对我区梨树栽培具有积极作用。本试验以日本和中国的23个亚洲梨品种为研究对象。根据获得的值,对品种进行分类,选择适合捷克共和国条件的品种。早熟品种“新精”和“早素里”因其清爽多汁、可塑性强、易于栽培而被证明是有前途的品种。需求普遍较低。“Shinseiki”果实大小适中(平均103克)。所评价的品种“早苏里”果实的大小可以达到非常大的大小(平均255克)。适合捷克条件的中成熟品种是“Hosui”和“砀山苏李”(平均9月11日至19日成熟)以及另一个有趣的品种“Kirgizkaja zimnaja”。其他适合的中成熟品种有‘nijisisseiki’、‘沙里’和‘Pung Su’。在评估的晚熟品种中,“芽梨”和“木陈”具有较高的冷藏性,具有较好的应用前景。
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引用次数: 2
Selection and micropropagation of valuable caper genotypes 有价值的刺山柑基因型的选择与微繁
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-06-30 DOI: 10.17221/40/2019-hortsci
F. Sottile, N. Giuggioli, D. Marinoni, C. Peano, M. Signore
The high quality of the various biotypes present in the natural or cultivated state is one of the main features for caper production. Up to now, however, no selection activity has been carried out in order to identify the most suitable accessions for providing better quality products. In this paper, we report the first results of a selection of caper genotypes characterised by significant qualitative traits. A micropropagation protocol was evaluated in order to improve and allow the multiplication of the most promising Capparis spinosa L. subsp. spinosa genotypes, selected in Salina (Aeolian Islands), Sicily (Italy), in collaboration with the most important local growers.
在自然或栽培状态下,各种生物型的高品质是槟榔生产的主要特征之一。然而,到目前为止,还没有开展任何选择活动,以确定最合适的产品,以提供更优质的产品。在这篇论文中,我们报告了第一个以显著的质量性状为特征的刺山柑基因型的选择结果。为了提高和促进最有前途的刺毛椒子的增殖,对一种微繁方案进行了评价。与当地最重要的种植者合作,在西西里岛(意大利)的萨利纳(伊奥利亚群岛)选择了spinosa基因型。
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引用次数: 2
Morphological variation of male A. arguta plants affects their flowering potential and pollen efficiency 软豆雄株的形态变异影响其开花潜力和花粉效率
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-06-30 DOI: 10.17221/37/2019-hortsci
A. Stasiak, B. Łata, M. Bieniasz, P. Latocha
Actinidia arguta (Siebold et Zucc.) Planch. ex Miq. is functionally a cryptic dioecious plant and successful pollination is crucial for achieving high quality fruit. The extent and bases of morphological variability in female genotypes have been well studied, but here we focus on the males. Mature plants of seven male A. arguta genotypes were studied in 2016 and 2017 during which a suite of phenological and morphological features was measured on ten randomly chosen one-year-old canes on each plant. This analysis was complemented by two other, specialized measures potentially related to pollination efficiency, namely pollen quality, and quantity. The time of bud break was similar each year across all genotypes and the pollen quality was uniformly very high (viability – 95.0–99.9% and germination – 89.0–97.3%). However, the ten genotypes exhibited high variability in both the number of flowers per inflorescence and pollen quantity, indicating marked differences in their flowering potential and efficiency as pollinizers. The male kiwiberry indicators developed in this study – flowering potential and pollen efficiency, appear to be effective tools for the comparative evaluation of male A. arguta genotypes.
争论猕猴桃(Siebold et Zucc.)Planch。前Miq。在功能上是一种神秘的雌雄异株植物,成功授粉对获得高质量的果实至关重要。女性基因型形态变异的程度和基础已经得到了很好的研究,但这里我们关注的是男性。2016年和2017年,研究了七种雄性论证藻基因型的成熟植株,期间在每株植株上随机选择的十根一年生的甘蔗上测量了一套酚学和形态学特征。这一分析得到了另外两项可能与授粉效率有关的专门措施的补充,即花粉质量和数量。所有基因型的破芽时间每年都很相似,花粉质量也都很高(活力为95.0-99.9%,发芽率为89.0-97.3%)。然而,这十个基因型在每个花序的花数和花粉量方面都表现出很高的变异性,表明它们作为花粉器的开花潜力和效率存在显著差异。这项研究中开发的雄性猕猴桃指标——开花潜力和花粉效率,似乎是比较评估雄性乌鸡基因型的有效工具。
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引用次数: 2
Minimal morphoagronomic descriptors for Cuban pineapple germplasm characterisation 古巴凤梨种质特征的最小形态农艺描述符
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-03-31 DOI: 10.17221/27/2019-hortsci
Daymara Rodríguez-Alfonso, Miriam Isidrón-Pérez, O. Barrios, Z. Fundora, J. Hormaza, M. Grajal-Martín, L. Herrera-Isidrón
A set of minimum descriptors allow for the rapid characterisation of germplasm facilitating the conservation and use of plant material. The objective of this work was to establish a list of minimum descriptors to facilitate the morphological characterisation of the ex situ pineapple collection in Cuba. Therefore, 48 pineapple accessions were characterised according to the morphoagronomic descriptors established by the International Board for Plant Genetic Resources (IBPGR). The data were processed by Multivariate Analysis, where a Multiple Principal Components Analysis was used for the qualitative and quantitative traits. A list with 14 minimum descriptors was proposed. The leaf ’s colour, the thickness of the longest leaf, the distribution of the spines, the fruit shape, the fruit colour when ripe, the flesh colour, the weight of fruit flesh, eye form, the fruit height, the fruit diameter, the fruitlet shape, the core diameter, the total soluble solids of the fruit, and the crown weight/fruit weight ratio were selected as the minimum descriptors. Because most of the descriptors refer to the pineapple’s genetic improvement or commercialisation aspects, it could be a useful tool for scientists and producers.
一套最小描述符允许对种质资源进行快速表征,促进植物材料的保护和利用。这项工作的目的是建立一个最低描述符的清单,以促进古巴非原生境菠萝收集的形态特征。因此,根据国际植物遗传资源委员会(IBPGR)建立的形态农艺描述符对48份菠萝材料进行了鉴定。数据采用多变量分析,对定性和定量性状采用多主成分分析。提出了一个包含14个最小描述符的列表。选择叶的颜色、最长叶的厚度、刺的分布、果实形状、成熟时的果实颜色、果肉颜色、果肉重量、眼形、果实高度、果实直径、小果形状、果心直径、果实总可溶性固形物和冠重/果重比作为最小描述子。由于大多数描述符都涉及菠萝的遗传改良或商业化方面,因此它可能是科学家和生产者的有用工具。
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引用次数: 2
Evaluation of genetic fidelity of in vitro-propagated blackberry plants using RAPD and SRAP molecular markers 用RAPD和SRAP分子标记评价黑莓离体繁殖植株的遗传保真度
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-03-31 DOI: 10.17221/20/2019-hortsci
O. Borsai, M. Hârța, K. Szabo, C. Kelemen, Flavia Andreea Andrecan, M. Codrea, D. Clapa
The aim of this research was to evaluate the genetic uniformity of blackberry plants (Rubus fruticosus L. ‘Loch Ness’ and ‘Chester Thornless’) obtained by micropropagation. Genetic uniformity was analysed by using RAPD and SRAP markers. For the in vitro multiplication, the slightly modified Murashige and Skoog (1962) basal medium was used, supplemented with 0.5 mg/l 6-Benzyladenine (BA), prepared with tap water and 50 g/l wheat starch as the gelling agent. This culture medium ensured the regeneration of well-developed plantlets, with multiplication rates of more than 42 for both cultivars. The in vitro multiplication was carried out in 30 months including 12 subcultures. The plants obtained from the 3rd and 11th subcultures were compared with the mother plants using 64 SRAP primer combinations (eight forward and eight reverse primers) and 20 RAPD primers to check their genetic fidelity. The amplification products were monomorphic in the micropropagated plants and the mother plant. No polymorphism was detected, thus proving the genetic fidelity and uniformity of the micropropagated plants.
本研究的目的是评价黑莓植株(Rubus fruticosus L. ' Loch Ness '和' Chester Thornless ')的遗传均匀性。利用RAPD和SRAP标记分析遗传均匀性。体外增殖采用稍作修饰的Murashige和Skoog(1962)基础培养基,添加0.5 mg/l 6-苄基ladenine (BA),以自来水和50 g/l小麦淀粉为胶凝剂制备。该培养基确保了发育良好的植株再生,两个品种的增殖率均超过42%。体外增殖30个月,包括12次传代培养。利用64个SRAP引物组合(8个正向引物和8个反向引物)和20个RAPD引物组合与母株进行遗传保真度比较。扩增产物在微繁植株和母株中呈单态。未检测到多态性,从而证明了微繁殖植株的遗传保真度和均匀性。
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引用次数: 14
Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism 苹果材料氨基环丙烷-1-羧酸合成酶基因(ACS1)多态性的分子特征
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-03-31 DOI: 10.17221/83/2018-hortsci
Khethani G. Mhelembe, Johan Kriel, C. Bester, E. Louw, R. Roodt‐Wilding, K. Tobutt
The ARC apple gene bank collection was genotyped for the fruit expressed gene ACS1, in which a short-interspersed element (SINE) in the promoter is known, when homozygous, to correlate with the delayed ethylene production. Primers were designed amplifying products less than 500 bp and 224 cultivars of domestic apple were analysed, 169 not previously genotyped. Of these, 82 were aa (homozygous for the high ethylene allele at 202 bp), 73 were ab and 14 bb (homozygous for the low ethylene allele, with the SINE, at 339 bp). The difference between the allele sizes, 137 bp, observed in the current study is consistent with the indel of 138 bp originally described, but differs considerably from the indel of 166 bp reported in literature. In addition, 21 accessions of other Malus species were analysed. Only one, M. ‘Golden Hornet’, had the b allele, which suggests it may have been introgressed from M. pumila.
ARC苹果基因库集合是针对果实表达基因ACS1进行基因分型的,其中启动子中的短穿插元件(SINE)是已知的,当纯合时,与延迟的乙烯产生相关。设计引物扩增小于500bp的产物,分析了224个国产苹果品种,其中169个以前没有进行基因分型。其中,82个是aa(202bp的高乙烯等位基因纯合),73个是ab和14bb(339bp的低乙烯等位蛋白SINE纯合)。在当前研究中观察到的等位基因大小之间的差异(137bp)与最初描述的138bp的indel一致,但与文献中报道的166bp的indell有很大不同。此外,还对其他苹果属植物的21份材料进行了分析。只有一个M.Golden Hornet具有b等位基因,这表明它可能是从M.pumila中渗入的。
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引用次数: 1
Effect of plant density on stem and flower quality of single-stem ornamental sunflower genotypes 植株密度对单茎观赏向日葵基因型茎和花品质的影响
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-03-31 DOI: 10.17221/10/2019-hortsci
E. Mladenović, S. Cvejić, S. Jocić, Nemanja Ćuk, J. Čukanović, M. Jocković, Ana Marjanović Jeromela
The aim of this research was to determine the optimum planting density for the production of high-quality cut flowers with desirable characteristics. 25 single-stem ornamental sunflower genotypes were planted at different densities and evaluated for flowering time, flower diameter, and stem circumference and length over a two-year production cycle. Three spacing patterns were used: 25 × 25 cm, 30 × 30 cm, and 70 × 30 cm, which led to the planting densities of 160 000, 90 000, and 60 000 plants/ha, respectively. The plant density had the most important effect on the stem circumference, flower diameter, and stem length (total variation 52, 60, and 58%, AMMI analysis) and a small effect on the flowering time (total variation 1%, AMMI analysis). Based on environment-focused scaling, all high-density environments could be suitable for the production of single-stem sunflower genotypes. The results demonstrated the adaptation of several sunflower genotypes G9, G11, G12, G21, and G22 as the most suitable based on the optimum flower diameter, stem circumference, and stem length. These results may lead to progress in growing ornamental sunflowers as a cut flower.
本研究的目的是确定生产具有理想特性的高质量切花的最佳种植密度。25种单茎观赏向日葵基因型以不同密度种植,并在两年的生产周期内评估开花时间、花径、茎周长和长度。采用三种间距模式:25×25 cm、30×30 cm和70×30 cm,种植密度分别为16万株、9万株和6万株/公顷。植物密度对茎围、花径和茎长的影响最为重要(总变异量52%、60%和58%,AMMI分析),对开花时间的影响较小(总变异率1%,AMMI研究)。基于以环境为重点的规模化,所有高密度环境都适合生产单茎向日葵基因型。结果表明,根据最佳花径、茎围和茎长,几种向日葵基因型G9、G11、G12、G21和G22的适应性是最合适的。这些结果可能有助于将观赏向日葵作为切花进行种植。
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引用次数: 1
Sustainable alternatives to chemicals for weed control in the orchard – a Review  果园杂草控制化学品的可持续替代品综述
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-03-31 DOI: 10.17221/29/2019-hortsci
Jebu Mia, F. Massetani, G. Murri, D. Neri
This review is designed to address various alternative weed-control practices and their possibilities in the fruit orchard in terms of sustainability. Correct weed management and maintenance of adequate orchard biodiversity are crucial for sustainable orchard soil management. The key is to practice an alternative weed-management approach (single or integrated) rather than to use possibly harmful chemicals only. Integration of modern equipment with a shallow tillage system can provide effective weed control in tree rows, including optimised tree performance and soil biodiversity. Living mulch suppresses weeds and enhances orchard biodiversity, while selection of less competitive and less pest-attracting species is crucial. Plastic covers offer long-term weed control, but additional nutrient amendments are required to maintain the balanced fertility of the soil. Wood chip mulch is suggested where the materials are available on or near the farm, and where there is lower incidence of perennial weeds. High pressure water and robotic systems are still in their infancy for fruit orchards, and required more research to confirm their efficiency.
本综述的目的是解决各种替代杂草控制的做法和他们的可能性在果园的可持续性方面。正确的杂草管理和保持足够的果园生物多样性对果园土壤的可持续管理至关重要。关键是采用替代的杂草管理方法(单一或综合),而不是只使用可能有害的化学品。现代设备与浅耕系统的集成可以有效地控制树行杂草,包括优化树木性能和土壤生物多样性。生物地膜抑制杂草生长,提高果园生物多样性,而选择竞争力较弱和害虫较少的物种是至关重要的。塑料覆盖可以长期控制杂草,但需要额外的养分修正来保持土壤的平衡肥力。建议在农场内或农场附近有材料且多年生杂草发生率较低的地方使用木屑覆盖。高压水和机器人系统在果园中仍处于起步阶段,需要更多的研究来证实它们的效率。
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引用次数: 36
Alleviation of allelochemical stress-induced growth inhibition and oxidative damage in lettuce under closed hydroponics through electro-degradation 电降解减轻化感化学胁迫对闭式水培生菜生长抑制和氧化损伤的影响
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-03-31 DOI: 10.17221/32/2019-hortsci
Md. Raihan Talukder, M. Asaduzzaman, M. Ueno, Hideyuki Tanaka, T. Asao
Successive lettuce cultivation in closed hydroponics using the same nutrient solution causes the excess production and accumulation of allelochemicals. The accumulated allelochemicals induce oxidative damage and lipid peroxidation in plants leading to growth inhibition. In this study, we investigated the allelochemicals that induced oxidative damage and lipid peroxidation in lettuce grown in a once used non-renewed nutrient solution (1NR) and a twice used non-renewed nutrient solution (2NR) obtained from the successive cultivation and the alleviation of these damages through electro-degradation (ED). The 1NR solution was used for six weeks for a one-time lettuce cultivation while the 2NR solution was used for twelve weeks for a two-times lettuce cultivation. The results showed that the allelochemical stress caused growth inhibition in the lettuce in both the 1NR and 2NR solutions. It was observed that there was a higher generation of H2O2 and O2. – as well as a lower activity of the antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD), and ascorbate peroxidase (APX) in the roots of the plants grown in both the 1NR and 2NR solutions compared to plants grown in the new nutrient solution. The higher level of lipid peroxidation due to the higher MDA (malondialdehyde) content and higher soluble protein content were also observed in the roots of those plants. It was evident that lettuce root damage occurred due to accumulation of the allelochemicals in the 1NR and 2NR solutions. These damaged roots could not function normally nor uptake water and minerals from the culture solution. As a result, retarded lettuce growth was observed in the 1NR and 2NR solutions. The oxidative damage, soluble protein content, lipid peroxidation and ultimately growth retardation were more pronounced in the plants grown in the 2NR solution compared to the plants grown in the 1NR solution. The application of ED to the 1NR and 2NR solutions maintained the plant growth through less oxidative damage, soluble protein production and lipid peroxidation as was observed in the plants grown with the new nutrient solution. Therefore, the ED of a non-renewed culture solution would alleviate the allelochemical stress in lettuce under recycled hydroponics.
在封闭水培中连续栽培莴苣,使用相同的营养液会导致化感物质的过量生产和积累。积累的化感物质引起植物氧化损伤和脂质过氧化,导致生长抑制。在本研究中,我们研究了生菜在一次使用的非更新营养液(1NR)和两次使用的非更新营养液(2NR)中诱导氧化损伤和脂质过氧化的化感化学物质,并通过电降解(ED)减轻了这些损伤。1NR溶液用于一次生菜栽培6周,2NR溶液用于两次生菜栽培12周。结果表明,在1NR和2NR溶液中,化感化学胁迫对生菜的生长均有抑制作用。观察到H2O2和O2的生成量较高。与在新营养液中生长的植物相比,在1NR和2NR溶液中生长的植物根部的抗氧化酶活性较低,如超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、愈创木酚过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)。由于MDA(丙二醛)含量和可溶性蛋白含量较高,这些植物的根中脂质过氧化水平也较高。结果表明,1NR和2NR溶液中化感物质的积累导致了生菜根系的损伤。这些受损的根不能正常发挥功能,也不能从培养液中吸收水分和矿物质。结果表明,在1NR和2NR溶液中,生菜生长迟缓。与1NR溶液相比,2NR溶液中生长的植株的氧化损伤、可溶性蛋白含量、脂质过氧化和最终的生长迟缓更为明显。在1NR和2NR溶液中添加ED,可以减少植物的氧化损伤、可溶性蛋白生成和脂质过氧化,从而维持植物的生长。因此,不更新培养液的ED可以缓解循环水培莴苣的化感化学胁迫。
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引用次数: 6
Long-term evaluation of rootstock effects on cropping and tree parameters of selected sweet cherry cultivars 砧木对甜樱桃品种种植和树木参数影响的长期评价
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-03-31 DOI: 10.17221/39/2018-hortsci
J. Blažková, A. Skřivanová, P. Suran, L. Zelený, F. Paprštein
The cropping of six sweet cherry cultivars that originated in the Research and Breeding Institute of Pomology at Holovousy, and a standard one, ‘Burlat’, were evaluated on three rootstocks in the period of 2007–2017. Trees planted in a spacing of 1.5 m × 5.0 m were trained as tall spindle axes utilising their natural tendency to develop a central leader. On the standard rootstock, P-TU-2, ‘Tim’ was the most productive with a mean total harvest of 47.6 kg per tree. ‘Sandra’ yielded the most on the PHLC rootstock with 56.2 kg per tree and ‘Helga’ yielded the most on Gisela 5 with a mean total harvest of 55.9 kg per tree. The mean impact of the rootstock on the tree vigour, measured upon the trunk cross section area, ranged from 148.4 cm2 on the standard rootstock P-TU-2 to 114.1 cm2 on the PHLC and 125.2 cm2 on Gisela 5 . On the standard rootstock P-TU-2, the most vigorous one according to this criterion was ‘Jacinta’ (178.0 cm2) whereas ‘Justyna’ (109.7 cm2) was the least vigorous. On the PHLC, the most vigorous was ‘Sandra’ (147.2 cm2) and the least was ‘Amid’ (94.0 cm2). The other tree characteristics were mainly dependant on the cultivar and minimally, or not at all, influenced by the rootstock vigour.
2007年至2017年期间,对原产于Holovousy果树研究与育种研究所的六个甜樱桃品种和一个标准品种“Burlat”在三个砧木上的种植进行了评估。以1.5 m×5.0 m的间距种植的树木被训练成高主轴,利用其自然倾向形成中心领导者。在标准砧木P-TU-2上,“蒂姆”产量最高,每棵树的平均总收获量为47.6公斤Sandra’在PHLC砧木上的产量最高,每棵树56.2公斤,Helga’在Gisela 5上的产量最大,平均每棵树总收获55.9公斤。根据树干横截面积测量,砧木对树木活力的平均影响范围从标准砧木P-TU-2的148.4cm2到PHLC的114.1cm2和Gisela 5的125.2cm2。在标准砧木P-TU-2上,根据该标准,最旺盛的砧木是“Jacinta”(178.0 cm2),而“Justyna”(109.7 cm2)最不旺盛。在PHLC上,活力最大的是“Sandra”(147.2 cm2),最小的是“Amid”(94.0 cm2)。其他树木特征主要取决于栽培品种,并且受砧木活力的影响最小,或者根本不受影响。
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引用次数: 3
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Horticultural Science
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