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Evaluation of ElecTIS bioreactor for the micropropagation of Malus sylvestris (L.) Mill., an important autochthonous species of Albania ElecTIS生物反应器对Malus sylvestris(L.)Mill。,阿尔巴尼亚的一种重要的本地物种
IF 1.2 Q3 HORTICULTURE Pub Date : 2021-03-31 DOI: 10.17221/69/2020-HORTSCI
V. Sota, C. Benelli, Brunilda Ҫuko, Elektra Papakosta, C. Depaoli, M. Lambardi, E. Kongjika
Malus sylvestris (L.) Mill., an economically-important fruit tree, is native to Albania and in many parts of Europe. It is cultivated as an ornamental tree, while its fruits are collected for food and a source of antioxidant substances. It is included in The IUCN Red List of Threatened Species. For these reasons, it is very important to optimise a micropropagation protocol, in order to obtain great numbers of clonal plantlets for ex situ conservation and production purposes. A liquid culture in a temporary immersion system (TIS) is a recently-proposed system for large-scale in vitro plant propagation. In this study, lateral buds of M. sylvestris were inoculated in MS medium with BAP (1 mg/L) and NAA (0.1 mg/L). In order to avoid oxidative stress, different antioxidants were previously tested with the culture in a gelled medium, and the combination of ascorbic acid and citric acid (both at 100 mg/L) was selected for the following culture in TIS. Stabilised explants were then cultivated in ElecTIS, an innovative TIS bioreactor, and in a semisolid medium, after which the two culture systems were evaluated. Overall, the ElecTIS showed to be more effective for all the tested parameters.
Malus sylvestris(L.)Mill。,一种经济上重要的果树,原产于阿尔巴尼亚和欧洲许多地区。它被作为一种观赏树种植,而它的果实被收集作为食物和抗氧化物质的来源。它被列入世界自然保护联盟濒危物种红色名录。出于这些原因,优化微繁殖方案是非常重要的,以便获得大量的克隆植株用于异地保护和生产目的。临时浸没系统(TIS)中的液体培养是最近提出的用于大规模体外植物繁殖的系统。本研究采用BAP(1mg/L)和NAA(0.1mg/L)的MS培养基接种樟子松侧芽。为了避免氧化应激,先前在凝胶培养基中用培养物测试了不同的抗氧化剂,并选择抗坏血酸和柠檬酸的组合(均为100mg/L)用于TIS中的以下培养。然后在创新的TIS生物反应器ElecTIS和半固体培养基中培养稳定的外植体,之后对两个培养系统进行评估。总体而言,ElecTIS对所有测试参数都更有效。
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引用次数: 5
Effects of LED and HPS lighting on the growth, seedling morphology and yield of greenhouse tomatoes and cucumbers LED和HPS照明对温室番茄和黄瓜生长、幼苗形态和产量的影响
IF 1.2 Q3 HORTICULTURE Pub Date : 2021-03-31 DOI: 10.17221/4/2020-HORTSCI
J. Dyśko, Kaniszewski Stanisław
In an experiment with tomato and cucumber transplants, light units equipped with purpose-built LED arrays were compared with HPS sodium lamps with a power of 600 W and a voltage of 230 V. For both the LED and HPS lamps, the same PAR radiation level was used at the plant height, which was about 70–80 μmol/m2/s in conditions without daylight. The supplementary lighting was carried out for 8 to 24 hours and was switched on during the day when the solar radiation outside the greenhouse was lower than 200 W/m2. The supplementary lighting with the LED and HPS lamps did not have a significant impact on the growth of the tomato and cucumber seedlings and the fresh and dry mass of the tomato and cucumber plants. The plants grown without the additional artificial lighting were significantly smaller in height, had fewer leaves, a smaller spread and produced lower fresh and dry weights. The tomato and cucumber plants grown under the LED lamps had a higher chlorophyll index than those grown under the HPS lamps and without any lighting. The supplementary lighting with the LED lamps increased the early yield of the tomatoes compared to the HPS and control plants but has no effect on the early yield of the cucumbers. Both the LED and HPS lighting significantly increased the total and marketable yield of the tomatoes and cucumbers.
在番茄和黄瓜移植实验中,将装有特制LED阵列的照明装置与功率为600 W、电压为230 V的HPS钠灯进行了比较。对于LED和HPS灯,在植物高度使用相同的标准杆数辐射水平,在无日光条件下约为70–80μmol/m2/s。补充照明进行了8至24小时,并在温室外的太阳辐射低于200W/m2的白天打开。LED和HPS灯的补充照明对番茄和黄瓜幼苗的生长以及番茄和黄瓜植株的新鲜和干燥质量没有显著影响。在没有额外人工照明的情况下生长的植物高度明显较小,叶片较少,分布较小,鲜重和干重较低。在LED灯下生长的番茄和黄瓜植株比在HPS灯下生长且没有任何照明的番茄和番茄植株具有更高的叶绿素指数。与HPS和对照植物相比,LED灯的补充照明提高了番茄的早期产量,但对黄瓜的早期产量没有影响。LED和HPS照明都显著提高了番茄和黄瓜的总产量和市场产量。
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引用次数: 7
Growth, decorative and nutritional values of ornamental cabbage (Brassica oleracea L.) in flowerbed conditions 观赏甘蓝(Brassica oleracea L.)在花坛条件下的生长、装饰和营养价值
IF 1.2 Q3 HORTICULTURE Pub Date : 2021-03-31 DOI: 10.17221/21/2020-HORTSCI
M. Błażewicz-Woźniak, Agnieszka Rybicka, M. Fil
Ornamental cabbages are cultivars of cabbages grown for decorative purposes due to the varied shape and colour of their internal leaves. The aim of the study was to determine the course of growth and to assess the decorative and nutritional value of two cultivars of ornamental cabbage (‘Pigeon Red’, ‘Coral Prince’) grown in a flowerbed along with annual plants: the French marigold (Tagetes patula nana L.) and flossflower (Ageratum houstonianum Mill.), using a pine bark mulch. The impact of the marigold and flossflower on the cabbage height and foliage changed with the plant growth. Cabbages growing without the proximity to the other species had the smallest diameter and formed the least leaves; and the plant’s height was lower in September. The cabbage height, foliage and leaf colour were modified by the weather in the subsequent years of research. The proximity of the marigold or flossflower and mulching of the soil with bark did not affect the colour of the leaves. The nutritional value of the ornamental cabbage was high, and the content of the mineral components in the leaves depended largely on their colour. The coloured leaves were more abundant in nitrogen, phosphorus, potassium and magnesium, while the green leaves contained more calcium and sulfur, and slightly more dry matter.
观赏卷心菜是一种卷心菜品种,由于其内部叶子的形状和颜色多种多样,因此用于装饰。本研究的目的是确定两种在花坛中生长的观赏卷心菜(“比根红”、“珊瑚王子”)以及一年生植物的生长过程,并评估其装饰和营养价值:法国万寿菊(Tagetes patula nana L.)和浮花(Ageratum houstonianum Mill.),使用松树皮覆盖物。金盏花和浮花对卷心菜高度和叶片的影响随植株生长而变化。在不靠近其他物种的情况下生长的卷心菜直径最小,叶片最少;9月份植株高度较低。在随后几年的研究中,卷心菜的高度、叶片和叶片颜色都受到了天气的影响。金盏花或浮花的接近程度以及用树皮覆盖土壤不会影响叶片的颜色。观赏白菜的营养价值高,叶片中矿物质成分的含量在很大程度上取决于其颜色。彩色叶片的氮、磷、钾和镁含量更丰富,而绿色叶片的钙和硫含量更高,干物质含量略高。
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引用次数: 1
Modelling the potential effects of climate change in the distribution of Xylotrechus arvicola in Spain 模拟气候变化对西班牙木鼠分布的潜在影响
IF 1.2 Q3 HORTICULTURE Pub Date : 2021-03-31 DOI: 10.17221/85/2019-HORTSCI
Á. M. Felicísimo, I. Armendâriz, Virginia Alberdi Nieves
Xylotrechus arvicola is an emerging grape pest that generates serious sanitary problems in vineyards and is currently expanding its range throughout Spain. The increasing prevalence of this pest in Spanish vineyards has been detected since 1990. In this study, the relationship between the climate and the actual distribution of the beetle was analysed, as well as how this distribution might change in the future according to several climate change models. The methodology was based on predictive models (SDM; species distribution modelling) using climate variables as explanatory factors, although the relationships were not necessarily causal. Maxent was used as the SDM method. The current climatic niche was calculated, and the actual potential distribution area was estimated. The relationships between the climate variables and the species probability of the presence were projected to various future climate change scenarios. The main conclusions reached were that climate change will favour the expansion of X. arvicola and that the potential infestation zones will be extended significantly. Although the results, because they were based on hypothetical climate frameworks that are under constant revision, were not conclusive, they should be taken into consideration when defining future strategies in the wine industry.
阿氏木霉是一种新出现的葡萄害虫,在葡萄园造成严重的卫生问题,目前正在西班牙各地扩大其范围。自1990年以来,人们发现这种害虫在西班牙葡萄园中的流行率越来越高。在这项研究中,根据几种气候变化模型,分析了气候与甲虫实际分布之间的关系,以及这种分布在未来可能如何变化。该方法基于预测模型(SDM;物种分布模型),使用气候变量作为解释因素,尽管这些关系不一定是因果关系。使用Maxent作为SDM方法。计算了当前的气候生态位,并估计了实际的潜在分布面积。气候变量和物种存在概率之间的关系被预测为未来各种气候变化情景。得出的主要结论是,气候变化将有利于阿维科拉的扩张,潜在的虫害区将大幅扩大。尽管这些结果是基于不断修订的假设气候框架,并不具有决定性,但在确定葡萄酒行业的未来战略时,应将其考虑在内。
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引用次数: 1
Mango fruit quality improvements in response to water stress: implications for adaptation under environmental constraints 芒果果实品质改善对水分胁迫的响应:环境约束下的适应意义
IF 1.2 Q3 HORTICULTURE Pub Date : 2021-03-31 DOI: 10.17221/45/2020-HORTSCI
V. H. Zuazo, D. F. Tarifa, B. C. Rodríguez, B. G. Ruíz, P. C. Sacristan, S. C. Tavira, I. García-Tejero
Mediterranean farming is facing increasing periods of water shortage and, in the coming decades, the water reduction is expected to exert the most adverse impact upon growth and productivity. This study was performed to assess the response of the physico-biochemical quality parameters of mango fruits to different doses of irrigation in a Mediterranean subtropical area in Spain. During two-monitoring seasons, trees were subjected to deficit-irrigation strategies receiving 33, 50, and 75% of a crop evapotranspiration (ETC), and a control at 100% ETC. According to the findings and respect to control, the yield was reduced in 8, 11, and 20% for the water-stressed trees at 75, 50, and 33% ETC, respectively, producing smaller fruits in line with the amount of applied irrigation. However, the water-stressed fruits significantly enhanced their quality, in particular at 33% ETC, with regards to the content of the health-promoting phytochemicals (total soluble solids, vitamin C, and β-carotenoids). Thus, sustainable water management without a detrimental effect on the yield could be possible, and farmers should be encouraged to adapt to the environmental constraints, producing improved quality fruits.
地中海农业正面临日益严重的缺水期,在未来几十年,缺水预计将对增长和生产力产生最不利的影响。本研究旨在评估西班牙地中海亚热带地区芒果果实的物理生化质量参数对不同灌溉剂量的响应。在两个监测季节,树木受到亏缺灌溉策略的影响,分别接受33%、50%和75%的作物蒸散量(ETC),并控制在100%ETC。根据研究结果和控制方面,水分胁迫的树木在75%、50%和33%的ETC下分别减产8%、11%和20%,根据灌溉量生产较小的果实。然而,在促进健康的植物化学物质(总可溶性固形物、维生素C和β-类胡萝卜素)的含量方面,水分胁迫的水果显著提高了它们的质量,特别是在33%的ETC下。因此,在不影响产量的情况下进行可持续的水资源管理是可能的,应该鼓励农民适应环境限制,生产出质量更高的水果。
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引用次数: 6
Grapevine Pinot gris virus infecting grapevines in Romania - Short Communicaiton 葡萄藤灰比诺病毒感染罗马尼亚的葡萄藤-简短通讯
IF 1.2 Q3 HORTICULTURE Pub Date : 2021-03-31 DOI: 10.17221/65/2020-HORTSCI
I. Guţă, E. Buciumeanu
Grapevine Pinot gris virus (GPGV) has been identified in many grape growing countries of the world since 2012. The aim of this work was to investigate the presence of GPGV on some accessions collected from a germplasm collection, in addition to the propagation material and clonal selection samples. During 2019–2020, a total of 199 samples have been analysed by a double antibody sandwich – enzyme-linked immunosorbent assay (DAS-ELISA) for the presence of GPGV, Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus-1+3 (GLRaV-1+3) and Grapevine fleck virus (GFkV). Among them, 107 samples (53.76%) showed a GPGV-infection, associated with or without symptoms on the leaves (deformations, chlorosis, mosaic, wrinkles) or stunting plants. The distribution of infected varieties showed a high rate of infection in old varieties (37.38%), followed by clones (32.71%), rootstocks (11.21%), clonal selections (9.35%) and new varieties (9.35%). The tests revealed the association of GPGV with GFkV (5 cases) and GLRaV-1+3 (2 cases). GPGV should be included in the rules of grapevine certification schemes for the production of virus-free mother plants.
自2012年以来,世界上许多葡萄种植国都发现了葡萄灰皮诺病毒(GPGV)。这项工作的目的是调查从种质收集的一些材料上,除了繁殖材料和克隆选择样本外,GPGV的存在。在2019年至2020年期间,共有199个样本通过双抗体夹心酶联免疫吸附测定法(DAS-ELISA)分析了GPGV、葡萄扇叶病毒(GFLV)、葡萄卷叶相关病毒1+3(GLRaV-1+3)和葡萄斑点病毒(GFkV)的存在。其中,107个样本(53.76%)显示GPGV感染,与叶片(变形、黄化、马赛克、皱纹)或发育迟缓的植物症状相关或无症状。感染品种分布以老品种感染率高(37.38%),其次为无性系(32.71%)、砧木(11.21%)、无性系选择(9.35%)和新品种(93.5%),发现GPGV与GFkV(5例)和GLRaV-1+3(2例)有关。GPGV应被纳入葡萄藤无病毒母株生产认证计划的规则中。
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引用次数: 3
Storage of proliferating gooseberry cultures under slow growth conditions 增殖醋栗培养物在缓慢生长条件下的储存
IF 1.2 Q3 HORTICULTURE Pub Date : 2021-01-26 DOI: 10.17221/142/2020-hortsci
D. Kucharska, T. Orlikowska, R. Maciorowski, M. Kunka, Angelika Niewiadomska-Wnuk
Short storage of in vitro cultures under slow-growth conditions is included in the commercial large-scale micropropagation process. It is dictated by the organizational scheme that provides temporary stop multiplication of shoots for some months. To avoid subculturing to fresh media every 4 weeks, which is obligatory for gooseberry, they can be kept in conditions that protect them from ageing, by slowing down their metabolism. To develop a rational schedule of gooseberry micropropagation, two experiments were used to adopt a temperature and length of time for storage. The best results were obtained with storage conditions at 2 °C for two or four months for proliferating cultures. Under these conditions, the percentage of necrotic shoots was low (< 10%), and shoot proliferation in the subsequent passages was at a level similar to proliferation cultures incubated in the growth room and sub-cultured monthly. The rate of shoots > 1 cm was higher than in the control in the growth room. Storage at 4 °C increased the probability of necrotic shoots up to 80% and decreased the number of all shoots and shoots > 1 cm in subsequent passages.
在缓慢生长条件下,体外培养物的短期储存包括在商业大规模微繁殖过程中。这是由组织方案决定的,该方案提供了几个月的临时停止芽的繁殖。为了避免每4周进行一次新鲜培养基的继代培养,醋栗可以通过减缓新陈代谢来保护它们免于老化。为了制定合理的醋栗微繁时间表,采用不同的贮藏温度和贮藏时间进行了两项试验。增殖培养物在2℃条件下保存2个月或4个月效果最好。在这些条件下,坏死芽的比例很低(< 10%),后续传代的芽增殖水平与生长室培养和每月继代培养的增殖培养水平相似。生长室内的芽苗生长速率高于对照。4°C贮藏使坏死芽的概率增加80%,随后传代中所有芽的数量减少,芽的直径为0 ~ 1 cm。
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引用次数: 1
‘Mini PS’: A new mini melon breeding line exploiting the “Dudaim” variability – Short Communication “迷你PS”:利用“Dudaim”短交变异性的迷你甜瓜新品种
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-12-30 DOI: 10.17221/86/2019-HORTSCI
G. Castro, G. Perpiñá, B. Picó, C. Esteras
‘Piel de Sapo’ is one of the most consumed market class of melons in the Mediterranean area and it represents an important economic crop in Spain. The ‘Mini PS’ melon breeding line, which bears two main introgressions from the dudaim ‘Queen’s pocket’ melon in the Piel de Sapo genetic background, was evaluated for its fruit quality traits in three environments. Some interesting commercial characteristics were detected, such as a notable decrease in the fruit weight and a rounder shape, compared with Piel de Sapo, while the other quality traits were not altered. Thus, this mini melon line, ideal as a personal melon, may be useful in the development of new melon cultivars.
“Piel de Sapo”是地中海地区消费最多的市场瓜类之一,它代表了西班牙重要的经济作物。以Piel de Sapo遗传背景下的杜达姆“女王口袋”甜瓜为主要遗传基因的“Mini PS”甜瓜选育品系,在3种环境下对其果实品质性状进行了评价。与Piel de Sapo相比,发现了一些有趣的商业特征,如果实重量明显减少,形状更圆,而其他品质性状没有改变。因此,这一小型甜瓜系作为一种理想的个人甜瓜,可能对培育甜瓜新品种有用。
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引用次数: 1
The influence of fungi of the Trichoderma genus on the flowering of Freesia refracta Klatt ‘Argentea’ in winter 木霉属真菌对阿根廷小苍兰冬季开花的影响
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-12-30 DOI: 10.17221/35/2019-hortsci
B. Janowska, R. Andrzejak, T. Kosiada
The flowering and quality of Freesia refracta Klatt ‘Argentea’ were assessed after the application of fungi of the Trichoderma genus and assimilation lighting. The assimilation lighting accelerated the flowering of the Freesia refracta ‘Argentea’ plants by 3–4 weeks. The fungi of the Trichoderma genus accelerated the flowering of the Freesia refracta ‘Argentea’ plants with light deficit by about one week. The assimilation lighting resulted in the development of shorter main inflorescence shoots regardless of the fact whether the plants had been treated with the fungi of the Trichoderma genus or not. The assimilation lighting and the fungi of the Trichoderma genus stimulated the development of lateral inflorescence shoots in the ‘Argentea’ cultivar. The fungi of the Trichoderma genus stimulated the development of flowers in the ‘Argentea’ cultivar. This effect was particularly noticeable when the plants were exposed to the assimilation lighting. The assimilation lighting stimulated the uptake of potassium in the ‘Argentea’ cultivar. The fungi of the Trichoderma genus stimulated the uptake of phosphorus and calcium in the plants underexposed to light. They also stimulated the uptake of potassium in the plants exposed to the assimilation lighting. The assimilation lighting stimulated the uptake of microelements. The fungi of the Trichoderma genus stimulated the uptake of iron, manganese and zinc both in the plants exposed to the assimilation lighting and those underexposed to light. The assimilation lighting combined with the treatment with the fungi of the Trichoderma genus stimulated the uptake of copper.
采用木霉属真菌和同化光照对小苍兰(Freesia refracta Klatt ' gentea)的开花和品质进行了评价。同化光照加速了小苍兰(Freesia refracta ' gentea ')植物3-4周的开花。木霉属的真菌加速了小苍兰(Freesia refracta ' argenttea ')的开花,使其在光照不足的情况下提前了大约一周。无论木霉属真菌处理与否,同化光照均使植株主花序短。同化光照和木霉属真菌促进了“银茶”品种侧花序芽的发育。木霉属的真菌刺激了“阿根廷茶”品种花的发育。当植物暴露在同化照明下时,这种效果尤其明显。同化光照刺激了‘银茶’品种对钾的吸收。木霉属真菌刺激了光照不足的植物对磷和钙的吸收。它们还刺激了暴露在同化光照下的植物对钾的吸收。同化光照促进了微量元素的吸收。木霉属真菌刺激了暴露于同化光照和光照不足的植物对铁、锰和锌的吸收。同化光照结合木霉属真菌处理促进了铜的吸收。
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引用次数: 4
A preliminary evaluation of the effects of pollinator enhancement and gibberellins on the fruit set and fruit shape of ‘Conference’ pears – Short Communication 增强传粉媒介和赤霉素对“会议”梨坐果和果形影响的初步评价——短通
IF 1.2 Q3 HORTICULTURE Pub Date : 2020-12-30 DOI: 10.17221/123/2019-hortsci
J. Smessaert, G. Kemenade, Anneleen Arnoys, O. Honnay, W. Keulemans
‘Conference’ (Pyrus communis L.) is a self-incompatible cultivar, although it can also set fruit parthenocarpically. Stimulating parthenocarpy through gibberellin (GA) applications increases the fruit set, but it may also negatively affect the fruit size and shape. The aim of this study was to investigate the effects of a bumblebee (BB) amendment in combination with a GA treatment on the fruit set and fruit shape of ‘Conference’ pears. In the first experiment, we applied three treatments (GA, GA + BB & control) in a ‘Conference’ monoculture. In the second experiment, we applied two treatments (GA & GA + BB) in a ‘Conference’ orchard inter-planted with ‘Concorde’ as pollinizer trees. Both experiments showed that the GA application and bumblebee supplementation did not affect the fruit set. However, the BB treatment resulted in significantly higher amounts of normally shaped pears. Trees closer to the bumblebee hive had more normal shaped pears than trees further away.
“会议”(Pyrus communis L.)是一种自交不亲和的品种,尽管它也可以单性结果。施用赤霉素(GA)刺激孤雌核可增加坐果量,但也可能对果实大小和形状产生负面影响。本研究的目的是研究大黄蜂(BB)改性剂与GA处理相结合对“会议”梨的坐果和果实形状的影响。在第一个试验中,我们在“会议”单一栽培中应用了三种处理(GA, GA + BB和对照)。在第二个试验中,我们在“会议”果园中间种了“协和”作为授粉树,采用了两种处理(GA和GA + BB)。两项试验均表明,GA施用和大黄蜂添加对坐果没有影响。然而,BB处理导致正常形状梨的数量显著增加。靠近大黄蜂蜂巢的树比远离蜂巢的树有更多正常形状的梨。
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引用次数: 1
期刊
Horticultural Science
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