Kessaya Radarit, Wan Wiriya, C. Chai-Adisaksopha, S. Chantara, T. Prapamontol
Limitations in reporting polycyclic aromatic hydrocarbons (PAHs) accumulated in humans arise from the impact of intense biomass burning and air pollution in upper Southeast Asia. This study analyzed PAHs in human hair samples and explored the associations between ambient PM2.5 and hair PAH concentrations. Follow-up hair collections were conducted on 111 female students, and hair samples with a length of 4 cm were analyzed. Ten different PAHs were identified in the subjects' hair samples. The most substantial pooled estimate of cumulative effects of PM2.5 exposure was observed at lag04 (4-month average). At lag04, every 10 μg/m3 increase in PM2.5 concentration was significantly associated with 0.33, 0.29, 0.35, 0.16, 0.04, 0.64, and 1.66 ng/g hair increases in Acy, Ace, Flu, Ant, Chr, BaP, and total PAHs, respectively (P < 0.05). The findings underscore the significant contribution of ambient PM2.5 exposure to the elevation of PAHs in human hair. Specifically, each centimeter of hair represents a one-month exposure to ambient PM2.5. Moreover, the accumulation of BaP, a carcinogenic PAH, significantly increased at heightened PM2.5 levels, emphasizing the importance of hair analysis in assessing PAH exposure risks and obtaining reliable and comprehensive health risk information. Keywords: Polycyclic aromatic hydrocarbons, Hair analysis, PM2.5
{"title":"Significantly Increased Accumulations of PAHs in Scalp Hair During Smoke-haze Period Among Female Adolescents in Chiang Mai, Thailand","authors":"Kessaya Radarit, Wan Wiriya, C. Chai-Adisaksopha, S. Chantara, T. Prapamontol","doi":"10.12982/nlsc.2024.013","DOIUrl":"https://doi.org/10.12982/nlsc.2024.013","url":null,"abstract":"Limitations in reporting polycyclic aromatic hydrocarbons (PAHs) accumulated in humans arise from the impact of intense biomass burning and air pollution in upper Southeast Asia. This study analyzed PAHs in human hair samples and explored the associations between ambient PM2.5 and hair PAH concentrations. Follow-up hair collections were conducted on 111 female students, and hair samples with a length of 4 cm were analyzed. Ten different PAHs were identified in the subjects' hair samples. The most substantial pooled estimate of cumulative effects of PM2.5 exposure was observed at lag04 (4-month average). At lag04, every 10 μg/m3 increase in PM2.5 concentration was significantly associated with 0.33, 0.29, 0.35, 0.16, 0.04, 0.64, and 1.66 ng/g hair increases in Acy, Ace, Flu, Ant, Chr, BaP, and total PAHs, respectively (P < 0.05). The findings underscore the significant contribution of ambient PM2.5 exposure to the elevation of PAHs in human hair. Specifically, each centimeter of hair represents a one-month exposure to ambient PM2.5. Moreover, the accumulation of BaP, a carcinogenic PAH, significantly increased at heightened PM2.5 levels, emphasizing the importance of hair analysis in assessing PAH exposure risks and obtaining reliable and comprehensive health risk information. Keywords: Polycyclic aromatic hydrocarbons, Hair analysis, PM2.5","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"115 29","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139616225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pitakchon Ponsawansong, T. Prapamontol, Jaras Singkaew, Guoxing Li, Xiaochuan Pan
Exposure to ambient particulate matter with an aerodynamic diameter ≤ 2.5 µm (PM2.5) has been associated with oxidative damage. This study investigated the PM2.5-bound polycyclic aromatic hydrocarbon (PAH) exposure levels during elevated PM2.5 levels and the oxidative damage among a preaging females in Chiang Mai, Thailand. The 64 female participants, aged between 50 and 65 years old, were followed for 5 weeks consecutively during the non-haze rainy season as the baseline and haze episodes. The morning void urine sample was collected weekly and analyzed for 8 species of hydroxylated-polycyclic aromatic hydrocarbons (OH-PAHs) and malondialdehyde (MDA). The results showed that the high increase in PM2.5 levels was positively associated with OH-PAH levels. In addition, for every 1 µg/gCre increase in individual urinary OH-PAH concentrations, the MDA concentration increased from 0.073 to 2.071 µg/gCre. Therefore, the present study suggested that detected urinary OH-PAHs were attributable to PM2.5-bound PAH exposure, particularly from inhalation. These study results provide useful information for policy makers and public health agencies to mitigate the chain effects of PM2.5 exposure from haze pollution. Keywords: Air pollution, Hydroxylated-PAHs, Oxidative stress, Urinary biomarkers
{"title":"Significantly Elevated Urinary OH-PAHs and Oxidative Damage Concentrations Attributable to PM2.5 Exposure: A Panel Study of Preaging Females in Chiang Mai, Thailand","authors":"Pitakchon Ponsawansong, T. Prapamontol, Jaras Singkaew, Guoxing Li, Xiaochuan Pan","doi":"10.12982/nlsc.2024.015","DOIUrl":"https://doi.org/10.12982/nlsc.2024.015","url":null,"abstract":"Exposure to ambient particulate matter with an aerodynamic diameter ≤ 2.5 µm (PM2.5) has been associated with oxidative damage. This study investigated the PM2.5-bound polycyclic aromatic hydrocarbon (PAH) exposure levels during elevated PM2.5 levels and the oxidative damage among a preaging females in Chiang Mai, Thailand. The 64 female participants, aged between 50 and 65 years old, were followed for 5 weeks consecutively during the non-haze rainy season as the baseline and haze episodes. The morning void urine sample was collected weekly and analyzed for 8 species of hydroxylated-polycyclic aromatic hydrocarbons (OH-PAHs) and malondialdehyde (MDA). The results showed that the high increase in PM2.5 levels was positively associated with OH-PAH levels. In addition, for every 1 µg/gCre increase in individual urinary OH-PAH concentrations, the MDA concentration increased from 0.073 to 2.071 µg/gCre. Therefore, the present study suggested that detected urinary OH-PAHs were attributable to PM2.5-bound PAH exposure, particularly from inhalation. These study results provide useful information for policy makers and public health agencies to mitigate the chain effects of PM2.5 exposure from haze pollution. Keywords: Air pollution, Hydroxylated-PAHs, Oxidative stress, Urinary biomarkers","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"7 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139616689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Tumbarski, N. Petkova, Ivan Ivanov, M. Todorova, Petya Ivanova, Lazar Nikolov, Neli Grozeva, Krastena Nikolova
The development of functional foods providing health benefits above the basic nutritional needs is of great interest to the food industry. This research aimed to: design functional yogurts with agave inulin and extracts from propolis, rose petals and Spirulina platensis; observe the physicochemical and microbiological changes during storage at 4°C for 21 days; evaluate the sensory characteristics of the new functional products. Three kinds of calcium alginate microcapsules (agave inulin + ethanolic propolis extract; agave inulin + ethanolic propolis extract + rose petals extract; agave inulin + ethanolic propolis extract + Spirulina extract) were prepared and applied in yogurts before the coagulation. During the storage, pH in all experimental groups gradually decreased (reaching values between 3.94 and 4.02 on the 21st day), which corresponded to the increasing titratable acidity (reaching values from 94.17°T to 108.78°T on the 21st day). The application of microcapsules did not affect the coagulation process and number of lactic acid bacteria in yogurts until the end of storage period. To the best of our knowledge, this is the first study to investigate the application of agave inulin, propolis, rose petals and S. platensis extracts in microencapsulated form in traditional Bulgarian yogurt in order to obtain a dairy product with enhanced functional properties. Keywords: Functional foods, Yogurt, Microencapsulation, Propolis, Rose petals, Spirulina platensis
{"title":"Design of Functional Yogurts with Microencapsulated Biologically Active Compounds","authors":"Y. Tumbarski, N. Petkova, Ivan Ivanov, M. Todorova, Petya Ivanova, Lazar Nikolov, Neli Grozeva, Krastena Nikolova","doi":"10.12982/nlsc.2024.011","DOIUrl":"https://doi.org/10.12982/nlsc.2024.011","url":null,"abstract":"The development of functional foods providing health benefits above the basic nutritional needs is of great interest to the food industry. This research aimed to: design functional yogurts with agave inulin and extracts from propolis, rose petals and Spirulina platensis; observe the physicochemical and microbiological changes during storage at 4°C for 21 days; evaluate the sensory characteristics of the new functional products. Three kinds of calcium alginate microcapsules (agave inulin + ethanolic propolis extract; agave inulin + ethanolic propolis extract + rose petals extract; agave inulin + ethanolic propolis extract + Spirulina extract) were prepared and applied in yogurts before the coagulation. During the storage, pH in all experimental groups gradually decreased (reaching values between 3.94 and 4.02 on the 21st day), which corresponded to the increasing titratable acidity (reaching values from 94.17°T to 108.78°T on the 21st day). The application of microcapsules did not affect the coagulation process and number of lactic acid bacteria in yogurts until the end of storage period. To the best of our knowledge, this is the first study to investigate the application of agave inulin, propolis, rose petals and S. platensis extracts in microencapsulated form in traditional Bulgarian yogurt in order to obtain a dairy product with enhanced functional properties. Keywords: Functional foods, Yogurt, Microencapsulation, Propolis, Rose petals, Spirulina platensis","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":" 1090","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139617506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Pitaksuteepong, Kitiya Kesornmalee Kesornmalee, Natthareephon Phanaphai
Oseltamivir phosphate (OSEL) is recommended for the prevention and treatment of influenza virus infection. In a hospital pharmacy, OSEL liquid extemporaneous preparation is generally compounded on a case-by-case basis from the capsules because it can be stored for a certain period. Therefore, the objective of the current research was to develop an oseltamivir reconstitutable dry suspension (ORDS), and to evaluate the stability of the ORDS. The dry-suspending vehicles were first developed and subjected to stability tests. The stable vehicle was selected to mix with the content of OSEL capsules and dry powder was termed ORDS. The stability of ORDS was then tested in both dried and reconstituted states. It was found that the dry vehicle using xanthan gum as a suspending agent (at 0.25% w/v of the reconstituted suspension) was the most stable formulation at 25°C and 45°C under 75% relative humidity (RH) for 28 days. Upon mixing with OSEL capsule contents, ORDS in the dried state showed no change in either physical or chemical properties after storing at room temperature for 7 days. For the reconstituted state, the ORDS showed no change in odor and pH after storing at 2°C to 8°C in a refrigerator, and 25°C and 45°C under 75%RH for 14 days. However, its color was slightly darker after storing at 45°C. The percentage remaining OSEL at the refrigerator temperatures was 98.13% ± 1.63%, at 25°C was 100.30% ± 0.71% and at 45°C was 97.54% ± 1.38%. In conclusion, ORDS may be a convenient alternative to extemporaneous preparations. Keywords: Oseltamivir capsule, Reconstitutable dry suspension, Extemporaneous, Physico-chemical stability, Influenza
{"title":"Development and Stability Study of Oseltamivir Reconstitutable Dry Suspension from Capsules","authors":"T. Pitaksuteepong, Kitiya Kesornmalee Kesornmalee, Natthareephon Phanaphai","doi":"10.12982/nlsc.2024.010","DOIUrl":"https://doi.org/10.12982/nlsc.2024.010","url":null,"abstract":"Oseltamivir phosphate (OSEL) is recommended for the prevention and treatment of influenza virus infection. In a hospital pharmacy, OSEL liquid extemporaneous preparation is generally compounded on a case-by-case basis from the capsules because it can be stored for a certain period. Therefore, the objective of the current research was to develop an oseltamivir reconstitutable dry suspension (ORDS), and to evaluate the stability of the ORDS. The dry-suspending vehicles were first developed and subjected to stability tests. The stable vehicle was selected to mix with the content of OSEL capsules and dry powder was termed ORDS. The stability of ORDS was then tested in both dried and reconstituted states. It was found that the dry vehicle using xanthan gum as a suspending agent (at 0.25% w/v of the reconstituted suspension) was the most stable formulation at 25°C and 45°C under 75% relative humidity (RH) for 28 days. Upon mixing with OSEL capsule contents, ORDS in the dried state showed no change in either physical or chemical properties after storing at room temperature for 7 days. For the reconstituted state, the ORDS showed no change in odor and pH after storing at 2°C to 8°C in a refrigerator, and 25°C and 45°C under 75%RH for 14 days. However, its color was slightly darker after storing at 45°C. The percentage remaining OSEL at the refrigerator temperatures was 98.13% ± 1.63%, at 25°C was 100.30% ± 0.71% and at 45°C was 97.54% ± 1.38%. In conclusion, ORDS may be a convenient alternative to extemporaneous preparations. Keywords: Oseltamivir capsule, Reconstitutable dry suspension, Extemporaneous, Physico-chemical stability, Influenza","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":" 91","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139619058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Benchawan Chamsai, Chutima Sinsuebpol, P. Opanasopit, W. Samprasit
This work aimed to develop mucoadhesive buccal films containing triamcinolone acetonide (TA), which shows local anti-inflammatory activity. A thin film layer was formulated from polyvinyl alcohol (PVA) by the solvent casting method. The solubility of TA and the ability of the co-solvents to produce films were evaluated. The optimal co-solvents were chosen to prepare the film. Pharmaceutical properties of the film, including uniformity of weight, thickness, mechanical property, wetting time, mucoadhesion, TA content, and release, were evaluated. Furthermore, the cytotoxicity and scratch assay were assessed for the in vitro cell line. Among the co-solvents, the system consisted of ethanol, distilled water, and an individual plasticizer (glycerin and polyethylene glycol 400 (PEG 400)), which showed promising co-solvents for TA solubility and film formation. The films were clear and colorless, with a smooth surface and good mechanical properties. PEG 400-contained film formulation had higher mechanical properties and faster wetting time than glycerin. Both films had similar mucoadhesive properties, although the TA content differed slightly, and a rapid release of TA from the films was observed. However, PEG 400-contained film offered a slightly faster TA release than glycerin. The films were non-toxic to gingival fibroblast cells. However, they decreased the cell growth and migration compared to the control. According to this research, co-solvents increased TA solubility and produced good characteristic buccal mucoadhesive films containing TA. Keywords: Triamcinolone acetonide, Co-solvents, Film, Mucoadhesive property
这项研究旨在开发含有曲安奈德丙酮（TA）的粘液粘附性口腔胶片，曲安奈德丙酮具有局部消炎活性。薄膜层由聚乙烯醇（PVA）通过溶剂浇铸法制成。对 TA 的溶解度和助溶剂制膜的能力进行了评估。选择了制备薄膜的最佳助溶剂。评估了薄膜的药用性能，包括重量均匀性、厚度、机械性能、润湿时间、粘附性、TA 含量和释放。此外，还评估了体外细胞系的细胞毒性和划痕试验。在助溶剂中，该系统由乙醇、蒸馏水和一种单独的增塑剂（甘油和聚乙二醇 400 (PEG400)）组成，这三种助溶剂对 TA 的溶解性和薄膜的形成具有良好的效果。薄膜透明无色，表面光滑，机械性能良好。与甘油相比，含 PEG 400 的薄膜配方具有更高的机械性能和更快的润湿时间。两种薄膜的粘附性相似，但 TA 含量略有不同，而且观察到 TA 从薄膜中快速释放。不过，含 PEG 400 的薄膜比甘油的 TA 释放速度稍快。薄膜对牙龈成纤维细胞无毒性。不过，与对照组相比，它们降低了细胞的生长和迁移。根据这项研究，共溶剂增加了 TA 的溶解度，并产生了含有 TA 的良好特性的口腔粘膜。关键词：曲安奈德曲安奈德；助溶剂；薄膜；粘附性
{"title":"Development of Mucoadhesive Buccal Films Containing Triamcinolone Acetonide by using Co-solvent Systems","authors":"Benchawan Chamsai, Chutima Sinsuebpol, P. Opanasopit, W. Samprasit","doi":"10.12982/nlsc.2024.012","DOIUrl":"https://doi.org/10.12982/nlsc.2024.012","url":null,"abstract":"This work aimed to develop mucoadhesive buccal films containing triamcinolone acetonide (TA), which shows local anti-inflammatory activity. A thin film layer was formulated from polyvinyl alcohol (PVA) by the solvent casting method. The solubility of TA and the ability of the co-solvents to produce films were evaluated. The optimal co-solvents were chosen to prepare the film. Pharmaceutical properties of the film, including uniformity of weight, thickness, mechanical property, wetting time, mucoadhesion, TA content, and release, were evaluated. Furthermore, the cytotoxicity and scratch assay were assessed for the in vitro cell line. Among the co-solvents, the system consisted of ethanol, distilled water, and an individual plasticizer (glycerin and polyethylene glycol 400 (PEG 400)), which showed promising co-solvents for TA solubility and film formation. The films were clear and colorless, with a smooth surface and good mechanical properties. PEG 400-contained film formulation had higher mechanical properties and faster wetting time than glycerin. Both films had similar mucoadhesive properties, although the TA content differed slightly, and a rapid release of TA from the films was observed. However, PEG 400-contained film offered a slightly faster TA release than glycerin. The films were non-toxic to gingival fibroblast cells. However, they decreased the cell growth and migration compared to the control. According to this research, co-solvents increased TA solubility and produced good characteristic buccal mucoadhesive films containing TA. Keywords: Triamcinolone acetonide, Co-solvents, Film, Mucoadhesive property","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139621623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aroon Jankham, W. Promprom, Wannachai Chatan, Kajita Somnate, Somsak Khambaione, P. Munglue
Star apple (Chrysophyllum cainito L.) has been used to treat several diseases. However, using this plant as a feed additive in aquaculture production is rare. Therefore, this work aimed to investigate the effects of dietary star apple peel extract (SAPE) on growth performance, intestinal histology, hematology, and non-specific immune parameters in common lowland frog (Rana rugulosa Wiegmann). Peel samples from star apple fruits were collected, dried, and extracted with methanol. Alkaloids, flavonoids, phenolics, saponins, tannins, and steroids were detected in SAPE at varied concentrations. Frogs of average weight 8.50 ± 0.10 g were fed diets incorporating 0, 10, 15, and 20 g SAPE/kg diet. After six weeks of feeding, the frogs' growth and physiological parameters were evaluated. The results revealed that the growth and intestinal histology features of frogs fed SAPE diets were significantly increased compared with the control (P <0.05). No significant differences among the treatments were observed in the survival rate, red blood cells, hematocrit, and hemoglobin (P >0.05). Serum albumin was significantly enhanced in frogs fed a 10 g SAPE/kg diet (P <0.05). Dietary SAPE significantly decreased serum alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, triglyceride, and cholesterol compared with the control (P <0.05). Furthermore, frogs fed SAPE diets had significantly elevated lysozyme activity, leukocyte respiratory burst activity, complement activity, and immunoglobulin M compared with the control (P <0.05). In conclusion, these results showed the beneficial effects of SAPE on the growth and health status of frogs with the appropriate level of 17.75 to 20.00 g/kg diet. Keywords: Medicinal plants, Common lowland frog, Growth performance, Immunity, Hematology
{"title":"Effects of Dietary Star Apple (Chrysophyllum cainito L.) Peel Extract on Growth Performance, Intestinal Histology, Hematology, and Non-Specific Immune Parameters in Common Lowland Frog (Rana rugulosa Wiegmann)","authors":"Aroon Jankham, W. Promprom, Wannachai Chatan, Kajita Somnate, Somsak Khambaione, P. Munglue","doi":"10.12982/nlsc.2024.009","DOIUrl":"https://doi.org/10.12982/nlsc.2024.009","url":null,"abstract":"Star apple (Chrysophyllum cainito L.) has been used to treat several diseases. However, using this plant as a feed additive in aquaculture production is rare. Therefore, this work aimed to investigate the effects of dietary star apple peel extract (SAPE) on growth performance, intestinal histology, hematology, and non-specific immune parameters in common lowland frog (Rana rugulosa Wiegmann). Peel samples from star apple fruits were collected, dried, and extracted with methanol. Alkaloids, flavonoids, phenolics, saponins, tannins, and steroids were detected in SAPE at varied concentrations. Frogs of average weight 8.50 ± 0.10 g were fed diets incorporating 0, 10, 15, and 20 g SAPE/kg diet. After six weeks of feeding, the frogs' growth and physiological parameters were evaluated. The results revealed that the growth and intestinal histology features of frogs fed SAPE diets were significantly increased compared with the control (P <0.05). No significant differences among the treatments were observed in the survival rate, red blood cells, hematocrit, and hemoglobin (P >0.05). Serum albumin was significantly enhanced in frogs fed a 10 g SAPE/kg diet (P <0.05). Dietary SAPE significantly decreased serum alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, triglyceride, and cholesterol compared with the control (P <0.05). Furthermore, frogs fed SAPE diets had significantly elevated lysozyme activity, leukocyte respiratory burst activity, complement activity, and immunoglobulin M compared with the control (P <0.05). In conclusion, these results showed the beneficial effects of SAPE on the growth and health status of frogs with the appropriate level of 17.75 to 20.00 g/kg diet. Keywords: Medicinal plants, Common lowland frog, Growth performance, Immunity, Hematology","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":" 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139625054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pipat Jittasai, Watchara Kanjanakawinkul, Artit Yawootti, W. Chaiyana
A significant amount of Rosa damascena Mill. leaves were discarded as waste during the harvesting and pruning for flowering. The current study aimed to investigate the potential of using these agricultural waste products in the cosmetics industry. Dried R. damascena leaves were extracted using maceration or environmentally friendly extractions, including infusion, digestion, ultrasonic, microwave, micellar, and pulsed electric fields (PEF) extraction. The extracts were analyzed for their chemical compositions and assessed for antioxidant, anti-tyrosinase, and anti-aging properties. The irritation profile of each extract was investigated by the hen’s egg-chorioallantoic membrane (HET-CAM) test. The results noted that extraction by ethanol yielded significantly higher extract content than deionized water (P < 0.05). However, total phenolic and flavonoid content was found to be greater, whereas the bioactive compounds, including rutin and rosmarinic acid, were only found in the aqueous extracts. On the other hand, kojic acid was found in the extract from infusion and maceration. Interestingly, the extracts from micellar and PEF extractions were found to significantly scavenge the radical, which were related to their rutin contents. Their collagenase inhibition (69.1 ± 10.1% and 54.8 ± 19.1%, respectively) were equivalent to those of epigallocatechin gallate, a well-known anti-aging compound (76.0 ± 1.2%). Besides, all extracts were safe since they induced no irritation in the HET-CAM test. In conclusion, environmentally friendly extraction of bioactive components from R. damascena leaves were suggested, with the potential to be exploited as an anti-aging and whitening ingredient for further use in cosmeceutical area. Keywords: Rosa damascene, Green extraction, Antioxidant, Anti-tyrosinase, Collagen, Elastin; Hyaluronan, Irritation
{"title":"Phytochemical Compositions and Cosmeceutical Activities of Rosa damascena Mill. Leaf Extracts from Environmentally Friendly Extraction Technique","authors":"Pipat Jittasai, Watchara Kanjanakawinkul, Artit Yawootti, W. Chaiyana","doi":"10.12982/nlsc.2024.008","DOIUrl":"https://doi.org/10.12982/nlsc.2024.008","url":null,"abstract":"A significant amount of Rosa damascena Mill. leaves were discarded as waste during the harvesting and pruning for flowering. The current study aimed to investigate the potential of using these agricultural waste products in the cosmetics industry. Dried R. damascena leaves were extracted using maceration or environmentally friendly extractions, including infusion, digestion, ultrasonic, microwave, micellar, and pulsed electric fields (PEF) extraction. The extracts were analyzed for their chemical compositions and assessed for antioxidant, anti-tyrosinase, and anti-aging properties. The irritation profile of each extract was investigated by the hen’s egg-chorioallantoic membrane (HET-CAM) test. The results noted that extraction by ethanol yielded significantly higher extract content than deionized water (P < 0.05). However, total phenolic and flavonoid content was found to be greater, whereas the bioactive compounds, including rutin and rosmarinic acid, were only found in the aqueous extracts. On the other hand, kojic acid was found in the extract from infusion and maceration. Interestingly, the extracts from micellar and PEF extractions were found to significantly scavenge the radical, which were related to their rutin contents. Their collagenase inhibition (69.1 ± 10.1% and 54.8 ± 19.1%, respectively) were equivalent to those of epigallocatechin gallate, a well-known anti-aging compound (76.0 ± 1.2%). Besides, all extracts were safe since they induced no irritation in the HET-CAM test. In conclusion, environmentally friendly extraction of bioactive components from R. damascena leaves were suggested, with the potential to be exploited as an anti-aging and whitening ingredient for further use in cosmeceutical area. Keywords: Rosa damascene, Green extraction, Antioxidant, Anti-tyrosinase, Collagen, Elastin; Hyaluronan, Irritation","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"57 29","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139382062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mitracarpus scaber is a tropical herb used in ethnomedicine for the treatment of inflammation and a variety of other diseases. The aim of this study was to develop a palm kernel-based emulgel containing Mitracarpus scarber and examine the product's physicochemical characteristics and anti-inflammatory efficacy on Wister rats. Different batches of emulgel comprising Mitracarpus scarber leaf extracts (MSE), different proportions of oils (palm kernel oil and/or liquid paraffin), and surfactants (tween-80 and/or Cremophor EL-30) were prepared. The freshly prepared and 5-month-old formulations, stored at room temperature (28 ± 2 °C), were evaluated for rheology, syneresis, and extract/excipient compatibility using Fourier transform infrared (FT-IR) spectroscopy. The spreadability, extrudability, and anti-inflammatory activity of the optimal formulation were compared with those of a commercial anti-inflammatory emulgel. Even after five months of storage, the created emulgels remained olive green and creamy, with a homogeneous texture, consistency, and glossy appearance. The optimized batch containing palm kernel oil, tween-80, and xanthan gum had the most stable characteristics, with no significant change in pH (P = 1.000) or FT-IR spectrum after 5 months of storage. It exhibited spreadability and extrudability of 12.75 cm2 and 600 g/cm2, respectively, compared to 16.82 cm2 and 400 g/cm2 for the commercial formulation. Four hours post-treatment, the anti-inflammatory effect of the optimized batch was significantly (P < 0.05) higher than that of the aqueous MSE dispersion. The obtained results demonstrate the prospects of palm kernel-based emulgel loaded with Mitracarpus scarber leaf extracts as an innovative therapeutic approach to inflammation treatment. Keywords: Inflammation, Emulgel, Mitracarpus scarber, Palm kernel oil, Formulation
{"title":"Development of a Palm Kernel-Based Emulgel Loaded with Mitracarpus scarber Leaf Extracts for Effective Anti-Inflammatory Activity","authors":"Zwanden Sule Yahaya","doi":"10.12982/nlsc.2024.001","DOIUrl":"https://doi.org/10.12982/nlsc.2024.001","url":null,"abstract":"Mitracarpus scaber is a tropical herb used in ethnomedicine for the treatment of inflammation and a variety of other diseases. The aim of this study was to develop a palm kernel-based emulgel containing Mitracarpus scarber and examine the product's physicochemical characteristics and anti-inflammatory efficacy on Wister rats. Different batches of emulgel comprising Mitracarpus scarber leaf extracts (MSE), different proportions of oils (palm kernel oil and/or liquid paraffin), and surfactants (tween-80 and/or Cremophor EL-30) were prepared. The freshly prepared and 5-month-old formulations, stored at room temperature (28 ± 2 °C), were evaluated for rheology, syneresis, and extract/excipient compatibility using Fourier transform infrared (FT-IR) spectroscopy. The spreadability, extrudability, and anti-inflammatory activity of the optimal formulation were compared with those of a commercial anti-inflammatory emulgel. Even after five months of storage, the created emulgels remained olive green and creamy, with a homogeneous texture, consistency, and glossy appearance. The optimized batch containing palm kernel oil, tween-80, and xanthan gum had the most stable characteristics, with no significant change in pH (P = 1.000) or FT-IR spectrum after 5 months of storage. It exhibited spreadability and extrudability of 12.75 cm2 and 600 g/cm2, respectively, compared to 16.82 cm2 and 400 g/cm2 for the commercial formulation. Four hours post-treatment, the anti-inflammatory effect of the optimized batch was significantly (P < 0.05) higher than that of the aqueous MSE dispersion. The obtained results demonstrate the prospects of palm kernel-based emulgel loaded with Mitracarpus scarber leaf extracts as an innovative therapeutic approach to inflammation treatment. Keywords: Inflammation, Emulgel, Mitracarpus scarber, Palm kernel oil, Formulation","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"58 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139390067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sri Charan Bindu Bavisetty, S. Karnjanapratum, Jaydeep Dave, Daniel Tua Purba Purba, T. Kudre, W. Maser, N. Maiyah, Passakorn Kingwascharapong, Ali Muhammed Moula Ali
This research explored the use of ultrasound as a pretreatment and extraction assisted process for extraction of collagen from seabass scales. Both acid-soluble (AC) and pepsin-soluble collagens (PC) were extracted with ultrasonication at different extraction times (24 and 48 h). Ultrasound pretreatment (PU) and ultrasound-assisted extraction (UE) notably improved AC extraction yields over 48 hours, registering 2.12% and 4.62%, respectively. The highest yield for PC, 21.85%, was achieved with 48 hours of ultrasound assisted extraction. Protein analysis verified the extracted collagens as type I, indicated by the presence of specific α and β chains. Further, FTIR spectra confirmed collagen's presence in all samples through distinct amide band peaks. Interestingly, ultrasound processes marginally reduced AC's thermal stability while boosting PC's thermal stability, especially when combined with pepsin treatment. Moreover, CD spectroscopy showed the preserved native structure of collagen across all samples. The ultrasound method increased collagen extraction yields and retained its structural integrity. These outcomes are pivotal for advancing collagen extraction methods in the biomedical and food sectors. Keywords: Seabass scales, Collagen, Ultrasonication, Molecular characterization, Physicochemical properties
本研究探索了使用超声波作为预处理和提取辅助工艺，从鲈鱼鳞片中提取胶原蛋白。在不同的提取时间（24 小时和 48 小时）下，用超声波提取了酸溶性胶原蛋白（AC）和胃蛋白酶溶性胶原蛋白（PC）。超声预处理（PU）和超声辅助提取（UE）显著提高了 48 小时内的 AC 提取率，分别为 2.12% 和 4.62%。超声辅助提取 48 小时后，PC 的产量最高，达到 21.85%。蛋白质分析表明，提取的胶原为 I 型，具体表现为存在特定的 α 和 β 链。此外，傅立叶变换红外光谱通过明显的酰胺带峰证实了胶原蛋白存在于所有样品中。有趣的是，超声处理略微降低了 AC 的热稳定性，而提高了 PC 的热稳定性，尤其是在结合胃蛋白酶处理的情况下。此外，CD 光谱显示所有样品都保留了胶原蛋白的原生结构。超声波方法提高了胶原蛋白的提取率，并保持了其结构的完整性。这些成果对于推动生物医学和食品领域的胶原蛋白提取方法至关重要。关键词鲈鱼鳞片 胶原蛋白 超声提取 分子表征 理化特性
{"title":"Ultrasonication on Collagen Yield, Physiochemical and Structural Properties from Seabass (Lates Calcarifer) Scales as Affected by Pretreatment and Extraction Conditions","authors":"Sri Charan Bindu Bavisetty, S. Karnjanapratum, Jaydeep Dave, Daniel Tua Purba Purba, T. Kudre, W. Maser, N. Maiyah, Passakorn Kingwascharapong, Ali Muhammed Moula Ali","doi":"10.12982/nlsc.2024.003","DOIUrl":"https://doi.org/10.12982/nlsc.2024.003","url":null,"abstract":"This research explored the use of ultrasound as a pretreatment and extraction assisted process for extraction of collagen from seabass scales. Both acid-soluble (AC) and pepsin-soluble collagens (PC) were extracted with ultrasonication at different extraction times (24 and 48 h). Ultrasound pretreatment (PU) and ultrasound-assisted extraction (UE) notably improved AC extraction yields over 48 hours, registering 2.12% and 4.62%, respectively. The highest yield for PC, 21.85%, was achieved with 48 hours of ultrasound assisted extraction. Protein analysis verified the extracted collagens as type I, indicated by the presence of specific α and β chains. Further, FTIR spectra confirmed collagen's presence in all samples through distinct amide band peaks. Interestingly, ultrasound processes marginally reduced AC's thermal stability while boosting PC's thermal stability, especially when combined with pepsin treatment. Moreover, CD spectroscopy showed the preserved native structure of collagen across all samples. The ultrasound method increased collagen extraction yields and retained its structural integrity. These outcomes are pivotal for advancing collagen extraction methods in the biomedical and food sectors. Keywords: Seabass scales, Collagen, Ultrasonication, Molecular characterization, Physicochemical properties","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"102 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139391095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The dairy cattle industry is expanding rapidly to accommodate the growing demand for human consumption. This results in high cattle feed production. This study aims to forecast the amount of maize residue and calculate the circularity level of using maize residue as a feedstock for total mixed ration feed production in dairy cattle feed production. The multiple linear regression analysis is performed to predict maize yields in Thailand so that maize residues can be calculated and used in feed production. The material circularity indicator is also used with biological cycles to assess the circularity level of dairy cattle feed production under the circular economy concept. The results show that the maize yield is expected to increase, resulting in more maize residues. The use of maize residues in daily cattle feed production gives the MCI value of 0.6038, representing a high degree of circularity and explaining the sustainable management of wastes in the cattle feed industry. Moreover, using maize residues as a part of feed ingredients instead of the Napier grass saves the cost by 14.2%. The study results provide a guideline for farmers and related authorities to plan for managing maize residue in daily cattle feed production to lower operation costs and minimize environmental problems. Keywords: Biological cycle, Circular economy, Dairy cattle feed production, Maize residues, Material circularity indicator, Multiple linear regression
{"title":"Prediction of Maize Yield and Assessment of the Material Circularity of Maize Residues Used in the Dairy Cattle Feed Production","authors":"N. Poolsawad","doi":"10.12982/nlsc.2024.006","DOIUrl":"https://doi.org/10.12982/nlsc.2024.006","url":null,"abstract":"The dairy cattle industry is expanding rapidly to accommodate the growing demand for human consumption. This results in high cattle feed production. This study aims to forecast the amount of maize residue and calculate the circularity level of using maize residue as a feedstock for total mixed ration feed production in dairy cattle feed production. The multiple linear regression analysis is performed to predict maize yields in Thailand so that maize residues can be calculated and used in feed production. The material circularity indicator is also used with biological cycles to assess the circularity level of dairy cattle feed production under the circular economy concept. The results show that the maize yield is expected to increase, resulting in more maize residues. The use of maize residues in daily cattle feed production gives the MCI value of 0.6038, representing a high degree of circularity and explaining the sustainable management of wastes in the cattle feed industry. Moreover, using maize residues as a part of feed ingredients instead of the Napier grass saves the cost by 14.2%. The study results provide a guideline for farmers and related authorities to plan for managing maize residue in daily cattle feed production to lower operation costs and minimize environmental problems. Keywords: Biological cycle, Circular economy, Dairy cattle feed production, Maize residues, Material circularity indicator, Multiple linear regression","PeriodicalId":132692,"journal":{"name":"Natural and Life Sciences Communications","volume":"27 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139390291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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