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Scale dependency of the effects of landscape structure and stand age on species richness and aboveground biomass of tropical dry forests 景观结构和林龄对热带干旱林物种丰富度和地上生物量影响的尺度依赖性
IF 1.7 4区 农林科学 Q2 FORESTRY Pub Date : 2023-08-31 DOI: 10.3832/ifor4239-016
G. Reyes-Palomeque, JM Dupuy, Carlos Portillo-Quintero, JL Andrade, FJ Tun-Dzul, JL Hernández-Stefanoni
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引用次数: 0
The effect of soil conditions on submountain site suitability for Norway spruce (Picea abies Karst.) in Central Europe 土壤条件对中欧挪威云杉亚山地立地适宜性的影响
IF 1.7 4区 农林科学 Q2 FORESTRY Pub Date : 2023-08-31 DOI: 10.3832/ifor4262-016
P. Samec, Jiří Volánek, L. Holík, Petra Rychtecká, Marie Balková, V. Vranová, Luigi Saulino
Norway spruce ( Picea abies [L.] Karst.) occurred rarely at submountain altitudes before reaching its present position as the most important economic tree species in Central Europe. Spruce cultivation outside of natural sites is under constant threat from harmful agents. In this study, we focused on the indication of potentially suitable planting sites for Norway spruce in areas of protected submountain (< 700 m a.s.l) populations using linear discriminant analysis (LDA) of soil properties in oak-beech, mixed and spruce stands in the territory of the Czech Republic. The soil A-horizon properties in mixed stands were more similar to those in spruce than in broadleaved stands; stand mixtures resulted in lower soil property separability than the localization of individual populations (57% and 85%, respectively). The suitable sites were defined by acid phosphomonoesterase > 151 µg hour -1 , soil porosity > 63%, aeration > 53%, bulk density < 0.86 g cm -3 , cation exchange capacity < 18 cmol + kg -1 , C org < 9% and N tot < 0.5% in clusters comprising the Bohemian highlands, North Bohemian rock cities and the Outer Western Carpathians. The LDA of soil properties offered a useful tool for assessing both naturalness and forest threats to support sustainable management.
挪威云杉(云杉)喀斯特(Karst.)在发展成为中欧最重要的经济树种之前,很少出现在亚山区。自然地点以外的云杉种植不断受到有害物质的威胁。在这项研究中,我们利用线性判别分析(LDA)对捷克境内的橡树山毛榉、混合和云杉林分的土壤特性进行分析,重点研究了在亚山区(海拔< 700米)种群受保护地区挪威云杉的潜在适宜种植地点。混交林土壤a层特征与云杉林相似,与阔叶林相似;林分混交林的土壤性质可分性低于单一种群的可分性(分别为57%和85%)。在波希米亚高地、北波希米亚岩石城市和西部喀尔巴阡山脉的集群中,酸性磷酸单酯酶> 151µg hour -1,土壤孔隙度> 63%,通气性> 53%,容重< 0.86 g cm -3,阳离子交换容量< 18 cmol + kg -1,碳价< 9%,氮价< 0.5%。土壤性质的LDA为评估天然性和森林威胁提供了一个有用的工具,以支持可持续管理。
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引用次数: 0
Evaluation of operating cost management models for selection cutting in Scandinavian continuous cover forestry 斯堪的纳维亚连续覆盖森林选伐经营成本管理模式评价
IF 1.7 4区 农林科学 Q2 FORESTRY Pub Date : 2023-08-31 DOI: 10.3832/ifor4204-016
S. Bianchi, A. Ahtikoski, Timo Muhonen, Eero Holmström, S. Valkonen, Y. Nuutinen, R. Picchio
The importance of continuous cover forestry (CCF) is increasing, yet there is lack of data and understanding about many aspects of this management, including the operational costs. Our objectives were to retrieve available harvesting cost models from published studies on selection cutting in Norway-spruce-dominated stands in Scandinavian countries and to evaluate them against real case studies. First, we retrieved three recently published harvesting cost models which provided explicit cost functions. Models 1 and 2, based on rotation forestry (RF) data and adapted for CCF, had separate sub-models for cutting and hauling costs. Model 3 was based on CCF data and produced total harvesting costs, including the cutting and hauling costs combined. Second, we measured cutting costs for 29 harvesting operations on stands with different stages of CCF structure. We then compared the observations with the simulations of Models 1 and 2 cutting cost sub-models for those cases. Third, we expanded the dataset, including a further 34 harvesting operations in stands with more advanced CCF structures (without measured costs). We then simulated the total harvesting costs for all three models in this dataset to investigate their general behaviour. On average, Models 1 and 2 cutting cost sub-models had relatively good and consistent predictions compared with the observed values. However, they differed in total costs due to different estimates for the hauling cost sub-models. Model 3 had predictions comparable to Models 1 and 2 in the more advanced stages of CCF, but much higher in the less advanced. This study provides important data regarding cutting costs in CCF and demonstrates the feasibility of using existing harvesting cost models.
持续覆盖森林(CCF)的重要性正在增加,但缺乏关于这种管理的许多方面的数据和了解,包括运营成本。我们的目标是从已发表的关于斯堪的纳维亚国家以挪威云杉为主的林分选择采伐的研究中检索可用的采伐成本模型,并根据实际案例研究对其进行评估。首先,我们检索了三个最近发表的收获成本模型,它们提供了明确的成本函数。模型1和模型2基于轮作林业(RF)数据并适应CCF,具有单独的采伐和运输成本子模型。模型3基于CCF数据,得出了总收获成本,包括切割和运输成本的总和。其次,我们测量了29种不同CCF结构阶段林分的采伐成本。然后,我们将这些观测结果与模型1和模型2削减成本子模型的模拟结果进行了比较。第三,我们扩展了数据集,在具有更先进的CCF结构的林分中增加了34个收获操作(没有测量成本)。然后,我们模拟了该数据集中所有三种模型的总收获成本,以调查它们的一般行为。平均而言,模型1和模型2的切割成本子模型与观测值相比具有较好的一致性预测。然而,由于对运输成本子模型的估计不同,它们在总成本上存在差异。模型3在CCF较晚期阶段的预测与模型1和模型2相当,但在较不晚期阶段的预测要高得多。本研究提供了降低CCF成本的重要数据,并证明了使用现有收获成本模型的可行性。
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引用次数: 0
Effective woody biomass estimation in poplar short-rotation coppices - Populus nigra × P. maximowiczii 杨树短轮伐林有效木本生物量估算——黑杨×大叶杨
IF 1.7 4区 农林科学 Q2 FORESTRY Pub Date : 2023-08-31 DOI: 10.3832/ifor4200-016
M. Šrámek, J. Weger, J. Bubeník, M. Matoušková, Klára Lengálová, R. Matula, P. Antov
Knowledge of the quantity of woody biomass of poplar short-rotation coppice (SRC) on agricultural land is a basic tool for management decisions like rotation length, volume production and the financial balance sheet of economic activities. The expansion of SRC requires a fast, reliable, easily applicable and cheap method for estimating the biomass yield, but existing methods are based on labour-demanding and lengthy measurements of all shoots per tree. The objective of this study was to verify a novel rapid biomass estimation method that uses averaged attributes of only a few largest shoots as a predictor variable for woody biomass in a poplar SRC, hybrid clone J-105 ( Populus nigra × P. maximowiczii ). Using data from 39 sample stumps with 187 shoots in total, we modelled shoot biomass as a function of an increasing number of shoots in interaction with different shoot parameters at two poplar SRC plantations. Results showed that the DBH of only three of the largest shoots per stump proved to be accurate estimators of the total shoot biomass of the individual stump. Comparison of biomass estimates at the stand level with a real amount of biomass indicated differences between 6-14%, depending on the site.
了解农业用地上杨树短轮伐灌木林(SRC)的木质生物量是制定轮作长度、产量和经济活动财务平衡表等管理决策的基本工具。SRC的扩展需要一种快速、可靠、易于应用和廉价的方法来估算生物量产量,但现有的方法是基于对每棵树的所有芽的劳动要求和漫长的测量。本研究的目的是验证一种新的快速生物量估算方法,该方法仅使用少数最大芽的平均属性作为预测因子,预测杨树SRC杂交无性系J-105 (Populus nigra × P. maximowiczii)的木质生物量。利用39个样桩共187个枝条的数据,在不同枝条参数的相互作用下,模拟了两个杨树SRC人工林的枝条生物量随枝数增加的函数。结果表明,每根树桩中最大的3个芽的胸径可以准确地估计单个树桩的总芽生物量。林分水平的生物量估计值与实际生物量的比较表明,根据立地的不同,差异在6-14%之间。
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引用次数: 0
A primary evaluation of Syrian forest damage since 2011: a case study of Alhamam and Alboz forest sites 2011年以来叙利亚森林破坏的初步评估:以Alhamam和Alboz森林遗址为例
4区 农林科学 Q2 FORESTRY Pub Date : 2023-06-30 DOI: 10.3832/ifor4032-016
M Aldakhil, S Abdullateef, F Mahmoud, A Alhasan, A Lakmes, M Al Abdullah, GR Watmough
Forests and trees make vital contributions to well-being, economic activities, ecosystem functioning and global system dynamics. There has been a lack of studies on the evaluation of the drivers of Syrian forest damage during the current conflict. This study estimated the damage since 2011 in the Alhamam and Alboz forest sites in Idlib province of Syria and evaluated the drivers of this damage. A multidimensional approach to damage assessment was developed which combined forest plot inventories, remotely sensed satellite image analysis, questionnaires and focus groups to enable a comparison of the forest from 2011 and 2017. The plot inventory data and remote sensing analysis provided estimates of forest damage and loss whilst focus groups and questionnaires provided vital information on the drivers of forest loss which is required for developing reforestation programmes. Overall, forest inventory data and satellite image analysis results indicated a reduction in tree cover, density and plant diversity. The results indicate a much higher loss of forest than is available from global products such as Global Forest Watch due to the prevalence in Syria of low-density Oak forests such as Alhamam not being classed as forest. The results of questionnaires and focus groups showed that the main cause of damage was an increased demand for fire-wood driven by a lack of fuel oil and increased unemployment, reduced household incomes and general price rises, making fuel more unaffordable. Respondents to surveys generally understood the importance of trees and forests and overtime the local knowledge of the importance of forest resources had not changed. This multidimensional approach indicates a reduced capacity of institutions to protect forests and will help to establish strategic plans to serve and protect the forest in the future.
森林和树木对人类福祉、经济活动、生态系统功能和全球系统动态作出了重要贡献。在当前冲突期间,缺乏对叙利亚森林破坏驱动因素的评估研究。本研究估计了自2011年以来叙利亚伊德利卜省Alhamam和Alboz森林遗址的破坏情况,并评估了这种破坏的驱动因素。开发了一种多维损害评估方法,将森林样地调查、遥感卫星图像分析、问卷调查和焦点小组相结合,对2011年和2017年的森林进行了比较。小区清查数据和遥感分析提供了对森林破坏和损失的估计,而焦点小组和调查表提供了关于森林损失驱动因素的重要信息,这是制定再造林方案所必需的。总体而言,森林清查数据和卫星图像分析结果显示树木覆盖、密度和植物多样性减少。结果表明,由于叙利亚普遍存在Alhamam等未被归类为森林的低密度橡树林,因此森林损失比全球森林观察等全球产品所提供的要高得多。问卷调查和焦点小组的结果表明,造成损害的主要原因是由于缺乏燃料油和失业增加、家庭收入减少和一般价格上涨导致对木柴的需求增加,使燃料更加难以负担。调查对象普遍了解树木和森林的重要性,而且随着时间的推移,当地对森林资源重要性的认识没有改变。这种多层面的做法表明,各机构保护森林的能力有所下降,并将有助于制定今后服务和保护森林的战略计划。
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引用次数: 0
Evaluation of urban forest spatial distribution characteristics in Guangdong - Hong Kong - Macao Greater Bay Area 粤港澳大湾区城市森林空间分布特征评价
IF 1.7 4区 农林科学 Q2 FORESTRY Pub Date : 2023-06-30 DOI: 10.3832/ifor4268-016
Qing Zhao, Chi Zhang, R. Hu, Wanhui Qian, Yuhan Wei, R. Lafortezza
To understand the health and ecological benefits of scenic recreational forests, we elucidated their spatial distribution characteristics, which can be used to create guidelines and reveal strategic issues regarding the spatial distribution of tree species. We randomly set up 900 m 2 quadrats in scenic recreational forest communities in Guangzhou, Foshan, and Zhuhai, and surveyed each tree using LiDAR. We then calculated the living vegetation volume (LVV) and amount of recreational space on the forest floor (RSFF), and analyzed the differences in spatial distribution characteristics across cities, locations, and forest types. The spatial distribution characteristics of trees differed between different cities, but were similar among different locations and forest types. Urban scenic recreational forest areas are thus configured based on aesthetics, recreational functions, and the spatial distribution characteristics of different tree species. Additionally, the relationship between the tree crown LVV and the RSFF was generally synergistic, yet contradictory. Although an increase in LVV can effectively improve ecological benefits, it may also reduce RSFF and other benefits provided by tree crowns to urban residents.
为了了解风景游憩林的健康和生态效益,我们阐明了其空间分布特征,可用于制定树种空间分布的指导方针和揭示战略问题。在广州、佛山、珠海3个景区休闲森林社区随机设置900 m2样方,利用激光雷达对每棵树进行测量。在此基础上,计算了森林地表活植被体积(LVV)和游憩空间(RSFF),并分析了不同城市、不同地点和不同森林类型的空间分布特征差异。不同城市间树木的空间分布特征不同,但不同地点、不同森林类型间树木的空间分布特征相似。城市风景游憩林区是根据审美、游憩功能和不同树种的空间分布特征来配置的。此外,树冠LVV与RSFF之间的关系总体上是协同的,但也存在矛盾。LVV的增加虽然可以有效提高生态效益,但也可能降低林冠为城市居民提供的RSFF等效益。
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引用次数: 0
Exposure elevation and forest structure predict the abundance of saproxylic beetles’ communities in mountain managed beech forests 暴露海拔和森林结构可预测山毛榉林区腐木甲虫群落的丰度
IF 1.7 4区 农林科学 Q2 FORESTRY Pub Date : 2023-06-30 DOI: 10.3832/ifor4264-016
F. Parisi, Adriano Mazziotta, E. Vangi, R. Tognetti, D. Travaglini, M. Marchetti, G. D’Amico, S. Francini, Costanza Borghi, G. Chirici, M. Borghetti
In the managed beech forests of Central Italy (Molise), the diversity of saprox-ylic species is potentially under threat by intensive management. To evaluate the impact of forestry on the biodiversity of these ecosystems, we analyzed the relationship between abundance of saproxylic beetles and (i) forest stand exposure and elevation, (ii) deadwood availability (coarse woody debris – CWD – and stumps), (iii) abundance of microhabitats. Four sampling sectors with different altitudes and exposure were identified in a 400-ha study area in the Appenine mountains. Fifteen circular plots (13 m radius) were established in each sector where deadwood and microhabitats were surveyed and saproxylic beetles sampled. We fitted joint species distribution models to quantify the relationship between forest attributes and saproxylic species’ abundance, including the interactions with their family and trophic category. Overall, 2334 specimens belonging to 64 species of saproxylic beetles were collected. Both abundance and species richness were higher in the sectors with high elevation (respectively, 55% and 44%) and South exposure (respectively, 28% and 44%). Average deadwood volumes were low (stumps: 7.6 m 3 ha -1 ; CWD: 0.3 m 3 ha -1 ; snags: 0.4 m 3 ha -1 ), and insect galleries were the most abundant microhabitat (380 records over a total of 434). The most important variables affecting abundance were stump characteristics (model deviance = 81.2), elevation (de-viance = 64.7), and CWD characteristics (deviance = 58.0). Our results show that topographical variables and forest structure jointly affect the abundance patterns of saproxylic beetle communities in managed beech forests. These ecological interactions imply that management has different impacts on the saproxylic communities in different topographic conditions. To acknowledge this complexity we advocate for a landscape-level forest management supporting the local beetle diversity maintaining a mosaic of semi-natural forest characteristics in different topographic contexts. The ecological value of the forest landscape will be further enhanced by the application of closer-to-nature management interventions based on deadwood retention, microhabitat creation and tree retention, in line with the guidelines of the new EU Forest Strategy for 2030.
在意大利中部(Molise)管理的山毛榉森林中,由于集约化管理,山毛榉物种的多样性可能受到威胁。为了评估林业对这些生态系统生物多样性的影响,我们分析了腐木甲虫丰度与(i)林分暴露和海拔、(ii)枯木可利用度(粗木屑- CWD -和树桩)、(iii)微生境丰度之间的关系。在亚平宁山区400公顷的研究区确定了四个不同海拔和暴露程度的采样区。在每个扇区建立15个圆形样地(半径13 m),调查枯木和微生境,取样腐木甲虫。拟合了联合物种分布模型,量化了森林属性与腐植酸物种丰度的关系,包括与其科和营养类别的相互作用。共采集腐木甲虫64种2334份标本。高海拔区(分别为55%和44%)和南暴露区(分别为28%和44%)的丰度和物种丰富度均较高。平均枯木量低(树桩:7.6 m 3 ha -1;CWD: 0.3 m 3 ha -1;微生境最丰富的是石缝(0.4 m 3 ha -1)和昆虫廊(380条记录,434条记录)。影响丰度的最重要变量是树桩特征(模型偏差= 81.2)、海拔(偏差= 64.7)和CWD特征(偏差= 58.0)。结果表明,地形变量和森林结构共同影响了管理山毛榉林腐木甲虫群落的丰度格局。这些生态相互作用表明,在不同的地形条件下,管理对腐殖酸群落有不同的影响。为了认识到这种复杂性,我们提倡景观级森林管理,支持当地甲虫多样性,在不同的地形背景下保持半自然森林特征的马赛克。根据新的《欧盟2030年森林战略》的指导方针,采用基于保留枯木、创造微生境和保留树木的更接近自然的管理干预措施,将进一步提高森林景观的生态价值。
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引用次数: 0
Response to climate and influence of ocean-atmosphere phenomena on annual radial increments of Pinus oocarpa Schiede ex Schltdl. & Cham in the Lagunas de Montebello National Park, Chiapas, Mexico 对气候的响应及海气现象对黄松径向年生长量的影响在墨西哥恰帕斯州拉古纳斯德蒙特贝罗国家公园
IF 1.7 4区 农林科学 Q2 FORESTRY Pub Date : 2023-06-30 DOI: 10.3832/ifor4112-016
LP Ponce-Calderón, J. Villanueva‐Díaz, D. Rodríguez-Trejo, B. Bilbao, Gdc Álvarez-Gordillo
The Lagunas de Montebello National Park, Chiapas, Mexico, is one of the most important protected areas in terms of ecology and the provision of ecosystem services in the state of Chiapas; however, it lacks long-term climate information to support comprehensive plans for the conservation of endangered species and ecosystem restoration actions. The objectives of this work were to analyze: (i) the interannual variability of the annual rings of Pinus oocarpa Schiede in the Lagunas de Montebello National Park, as influenced by climate; and (ii) the ocean-atmosphere phenomena influence on both the interannual variability of the total ring-width series. A dendrochronological series of P. oocarpa was built for the period 1857-2018 (162 years), which indicates the presence of extreme hydroclimatic events in the region. Of these, the 1998 drought was a milestone for the management of this natural area due to the high-intensity fires that affected the area and caused unprecedented ecological, social, and economic damage in the site history. The climatic variables with the greatest influence on the annual radial increase of the species are precipitation and maximum temperature in winter-spring, when growth is positively associated with precipitation and negatively associated with temperature, attributed to increases in evapotranspiration. El Niño Southern Oscillation was the phenomenon with the highest correlation with climatic variability and the radial growth of the species, in frequencies of less than five years, although the positive influence of the Atlantic Multidecadal Oscillation was also assessed annually. Global warming, characterized by increasing temperature, threatens the persistence of plant communities in the study area; therefore, knowing its impact on the growth of species of economic importance is essential to support conservation actions.
墨西哥恰帕斯州拉古纳斯蒙特贝罗国家公园是恰帕斯州最重要的生态和生态系统服务保护区之一;然而,它缺乏长期的气候信息来支持濒危物种保护和生态系统恢复行动的综合计划。这项工作的目的是分析:(i)受气候影响的拉古纳斯德蒙特贝罗国家公园oocarpa Schiede松年轮的年际变化;(ii)海洋-大气现象对总环宽序列的年际变化的影响。建立了1857-2018年(162年)的树年代学序列,表明该地区存在极端水文气候事件。其中,1998年的干旱是这一自然区域管理的一个里程碑,因为高强度的火灾影响了该地区,造成了该地区历史上前所未有的生态、社会和经济损失。对该树种径向年增长影响最大的气候变量是冬春季节的降水和最高温度,由于蒸散发的增加,生长与降水呈正相关,与温度负相关。厄尔Niño南方涛动是与气候变率和物种径向生长相关性最高的现象,频率不到五年,尽管每年也评估大西洋多年代际涛动的积极影响。以气温升高为特征的全球变暖威胁着研究区植物群落的持续性;因此,了解其对经济重要性物种生长的影响对支持保护行动至关重要。
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引用次数: 0
Fine root morphological traits and production in coniferous- and deciduous-tree forests with drained and naturally wet nutrient-rich organic soils in hemiboreal Latvia 拉脱维亚半北方地区具有排水和自然湿润营养丰富的有机土壤的针叶林和落叶林的细根形态特征和生产
IF 1.7 4区 农林科学 Q2 FORESTRY Pub Date : 2023-06-30 DOI: 10.3832/ifor4186-016
A. Bārdule, K. Polmanis, LL Krumšteds, A. Bārdulis, A. Lazdiņš
Fine root production is one of the key elements of carbon (C) turnover in soil in afforested peatlands and forest lands with organic soils. We estimated variability in fine root morphology traits and annual production in hemiboreal forests dominated by coniferous trees (Norway spruce) and deciduous trees (silver birch and black alder) with nutrient-rich organic soils in Latvia. In total, 23 research sites were established in drained and naturally wet forests of different ages, and ingrowth core techniques were used to sample fine roots and subsequently determine fine root morphology traits and annual production, and calculate C input through fine root litter. Significant differences in several fine root morphological traits between coniferous-and deciduous-trees-domi-nated stands were found. Fine root production tended to be higher in conifer-ous-trees-dominated stands and positively correlated with several forest stand characteristics: stand age, average tree diameter at breast height, basal area and average tree height, but negatively correlated with nitrogen and phosphorus content in soil. C input through fine root litter ranged up to 0.46 ± 0.16 t ha -1 yr -1 . It is necessary to conduct further research, including multi-annual estimates in a wider set of forest stands with diverse dominant tree species and growing conditions, to improve estimates, patterns and understanding of C flows through fine root litter in drained and naturally wet organic soils.
细根生产是泥炭林地和有机土壤林地土壤碳(C)周转的关键因素之一。我们估计了拉脱维亚营养丰富的有机土壤中以针叶树(挪威云杉)和落叶树(白桦和黑桤木)为主的半北方森林细根形态特征和年产量的变异。在不同树龄的排水和自然湿润森林中共建立23个研究点,采用长生核心技术对细根进行取样,测定细根形态特征和年产量,并通过细根凋落物计算C输入。针叶树和落叶树两种林分在几种细根形态特征上存在显著差异。以针叶树为主的林分细根产量较高,与林龄、平均胸径、基面积和平均树高呈正相关,与土壤氮磷含量呈负相关。细根凋落物的碳输入量为0.46±0.16 t / h -1 yr -1。有必要进行进一步的研究,包括在具有不同优势树种和生长条件的更广泛的林分中进行多年估算,以改进对排水和自然湿润有机土壤中细根凋落物中碳流动的估算、模式和理解。
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引用次数: 0
Identification of the ambrosia beetle Anisandrus dispar (Fabricius) (Coleoptera Curculionidae Scolytinae) using TaqMan™ probe assay on biological samples TaqMan™探针法在生物样品上鉴定ambrosia甲虫Anisandrus dispar (Fabricius)(鞘翅目Curculionidae Scolytinae)
IF 1.7 4区 农林科学 Q2 FORESTRY Pub Date : 2023-06-30 DOI: 10.3832/ifor4287-016
D. Rizzo, Andrea d'Agostino, Igor Stabile, Chiara Ranaldi, Andrea Marrucci, C. Zubieta, D. Lio, L. Bartolini, F. Pennacchio, E. Rossi, A. Garonna, Matteo Marchioro
The European shot-hole borer Anisandrus dispar (Fabricius) (Coleoptera Cur-culionidae Scolytinae) is a well-known ambrosia beetle living on shrubs and several ornamental and fruiting trees where it can cause heavy damages. Like other harmful xyleborine species, A. dispar can represent a potential threat outside its native region. Molecular diagnostic tools can lead to accurate identification of xylophagous species hidden in wooden matrix in phytosanitary surveys at entry points. A molecular assay based on qPCR TaqMan™ Probes was developed for the identification of A. dispar from different matrices. To setup and perform the test, DNA extraction was carried out from adults, larvae, and artificial samples of wood chips from oak healthy plants whose lysates were contaminated with a known amount of DNA of A. dispar adults. The assay has proven inclusive for A. dispar, and exclusive towards the non-target organisms, showing 100% analytical specificity. The limit of detection was 0.32 pg µL -1 for the samples of insect adult DNA of A. dispar , and at 0.8 pg µL -1 for the samples containing lysates of Quercus spp. and 0.1 ng µL -1 of A. dispar adult DNA. Repeatability and reproducibility showed low values independently from the matrix used for DNA extraction, confirming the possible use in diagnostics of biological samples even if not directly related to the presence of A. dispar developmental stages. The presented approach may be adjusted and applied for phytosanitary purposes to other quarantine pests and rapidly detect possible infestations in vegetal matrices globally traded
欧洲针孔虫Anisandrus dispar (Fabricius)(鞘翅目curculionidae Scolytinae)是一种众所周知的生活在灌木和一些观赏和果树上的ambrosia甲虫,它可以在那里造成严重的破坏。像其他有害的木草碱物种一样,异叶假单胞虫可以在其原生区域之外代表潜在的威胁。在入境口岸植物检疫调查中,分子诊断工具可以准确识别隐藏在木质基质中的木食物种。建立了一种基于qPCR TaqMan™探针的分子检测方法,用于鉴定来自不同基质的A. dispar。为了建立和执行测试,从橡树健康植物的成虫、幼虫和木屑的人工样本中提取DNA,这些橡树健康植物的裂解物被已知数量的a . dispar成虫DNA污染。该分析已被证明对异芽孢杆菌具有包容性,对非目标生物具有排他性,显示出100%的分析特异性。昆虫成虫DNA样品的检出限为0.32 pgµL -1,含有栎类裂解物的样品的检出限为0.8 pgµL -1,含有白棘成虫DNA的检出限为0.1 ngµL -1。可重复性和再现性显示出较低的值,独立于用于DNA提取的基质,证实了在生物样品诊断中的可能使用,即使与A. dispar发育阶段的存在没有直接关系。所提出的方法可以调整并应用于植物检疫目的,以其他检疫性有害生物,并快速检测全球交易的植物基质中可能发生的侵染
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Iforest - Biogeosciences and Forestry
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