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MX2 gene mRNA expression as potential biomarker for early pregnancy diagnosis in cattle 牛MX2基因mRNA表达作为早期妊娠诊断的潜在生物标志物
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-10-06 DOI: 10.56093/ijans.v93i10.138127
M K SHUKLA, None SHAMBHAVI, KOUSHLESH RANJAN, AMAN KUMAR, ATUL KUMAR VERMA, ASHUTOSH TRIPATHI, NEELESH KAPOOR, SURESH KUMAR
Early pregnancy diagnosis is vital for economic sustainability of dairy farms and maintaining the reproductive efficiency of the herd. There are many techniques including progesterone assay, pregnancy specific proteins and interferon stimulated genes have been explored for early pregnancy diagnosis but, they are associated with varying level of efficacy. In the present experiment, interferon stimulated gene (Myxovirus resistance gene 2/MX2) expression pattern was used as a potential biomarker for early pregnancy in cattle. The association of MX2 gene expression in relation to progesterone assay was studied to explore its potential use as biomarker of early pregnancy. The plasma progesterone concentration in conceived animals on day 7 (2.26±0.19 ng/ml), 17 (5.42±0.35 ng/ml) and 21(6.38±0.39 ng/ml) was recorded to be significantly higher as compared to respective values in non-conceived animals, i.e. 1.55±0.09 ng/ml, 4.14±0.14 ng/ml and 0.81±0.06 ng/ml. The sudden decrement in plasma progesterone concentration after day 17th discriminates conceived and non-conceived animals. MX2 expression levels were observed to spike in blood due to release of interferon tau (τ) after implantation of embryo. The relative mRNA expression of MX2 gene showed a 9.5 to 28.64-fold higher expression on 17 days post insemination in pregnant animals as compared to non-pregnant animals. Thus, MX2 gene can be used as a reliable biomarker for the early detection of pregnancy.
早期妊娠诊断对奶牛场的经济可持续性和维持牛群的繁殖效率至关重要。孕激素测定、妊娠特异性蛋白、干扰素刺激基因等技术已被用于妊娠早期诊断,但其疗效参差不齐。在本实验中,干扰素刺激基因(黏液病毒抗性基因2/MX2)表达模式被用作牛早期妊娠的潜在生物标志物。研究MX2基因表达与孕酮测定的关系,探讨其作为早期妊娠生物标志物的潜力。孕鼠第7天血浆孕酮浓度(2.26±0.19 ng/ml)、第17天(5.42±0.35 ng/ml)和第21天(6.38±0.39 ng/ml)均显著高于未孕鼠血浆孕酮浓度(1.55±0.09 ng/ml、4.14±0.14 ng/ml和0.81±0.06 ng/ml)。血浆孕酮浓度在第17天突然下降是怀孕和未怀孕动物的区别。胚胎着床后,由于干扰素tau (τ)的释放,血液中MX2的表达水平出现峰值。MX2基因mRNA的相对表达量在受精后17天妊娠动物比未妊娠动物高9.5 ~ 28.64倍。因此,MX2基因可作为妊娠早期检测的可靠生物标志物。
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引用次数: 0
Genetic diversity analysis of Katchaikatty Black – An endangered sheep breed from Tamil Nadu 泰米尔纳德邦濒危绵羊品种Katchaikatty Black的遗传多样性分析
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-10-06 DOI: 10.56093/ijans.v93i10.129633
REENA ARORA, ANAND JAIN, RAJA K N, MANDEEP KAUR, ASHISH KUMAR, SONIKA AHLAWAT, REKHA SHARMA
The study presents the genetic diversity of Katchaikatty Black, an endangered and culturally significant sheep breed of Tamil Nadu. A panel of 25 microsatellite markers recommended for Indian sheep was used for genotyping. Considerable genetic variation in terms of allele diversity and heterozygosity was observed within the investigated breed. A total of 174 distinct alleles were detected across the analyzed microsatellite loci with an average of 6.96 alleles per locus. The average observed and expected heterozygosity values were 0.600 and 0.706, respectively. The positive heterozygote deficiency (FIS) value of 0.171, estimated for this breed may be due to the possibility of Wahlund effect resulting from sampling from different breeding flocks, i.e. different villages and flocks in the same area. A normal L-shaped curve suggested absence of genetic bottleneck in this breed. The information generated will be useful in guiding conservation and management programmes for Katchaikatty Black sheep.
这项研究展示了Katchaikatty Black的遗传多样性,Katchaikatty Black是泰米尔纳德邦一种濒危的、具有重要文化意义的绵羊品种。一组推荐用于印度羊的25个微卫星标记被用于基因分型。在等位基因多样性和杂合性方面观察到相当大的遗传变异。在所分析的微卫星位点中共检测到174个不同的等位基因,平均每个位点有6.96个等位基因。平均观察杂合度和期望杂合度分别为0.600和0.706。估计该品种的阳性杂合子缺乏率(FIS)为0.171,可能是由于取样于不同种鸡群,即同一地区的不同村庄和不同种鸡群,可能存在Wahlund效应。正常的l型曲线表明该品种没有遗传瓶颈。所产生的信息将有助于指导Katchaikatty黑羊的保护和管理方案。
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引用次数: 0
Use of supracutaneous bone plating for management of radius/ulna and tibia/fibula fractures in dogs 皮上骨钢板治疗犬桡骨/尺骨及胫骨/腓骨骨折
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-13 DOI: 10.56093/ijans.v93i9.128389
AMIT KUMAR, ASHMEET KAUR, SUKHPREET SINGH, S P TYAGI, ADARSH KUMAR
The study was done with the objective to utilize supracutaneous bone plating as a mean of minimally invasive osteosynthesis for management of radius/ulna and tibia/fibula fractures in dogs. Nine cases of long bone fracture (2 radius/ulna and 7 tibia/fibula fracture) in dogs having body weight 8-20 kg and age 4 months to 2 years were repaired with supracutaneous bone plating (minimally invasive plate osteosynthesis) under general anaesthesia after a thorough physical, orthopaedic, neurological and radiographic examinations. Intra-operatively, 3.5 mm, locking head-limited contact-dynamic compression plate (LH-LC-DCP) was used in locking mode with insertion of two self-tapping locking head cortical screws of 3.5 mm (of varying length) in each proximal as well as distal fracture. The bone plate was applied on the medial surface of radius and tibia. Postoperative radiographs were taken to assessthe status of fracture reduction and fixation which was good in all the cases. Regular follow-ups showed good fracture reduction as well as fixation with no changes in implant position. Loosening of screws was observed in single case with no other complication in any of the cases. All the implants were removed on or before 68th day after surgery under sedation. All the animals showed good weight bearing with near to normal limb function on final day of the reappraisal. The supracutaneous bone constructs provided good reduction and fixation at fracture site similar as external skeletal fixators not compromising on axial and torsional stiffness.
本研究的目的是利用皮上骨钢板作为微创骨植入治疗犬桡骨/尺骨和胫骨/腓骨骨折的手段。对9例体重8 ~ 20 kg、年龄4个月~ 2岁的犬进行全身麻醉,经体格、骨科、神经学和影像学检查后,采用皮上骨钢板(微创钢板接骨术)修复长骨骨折(2例桡骨/尺骨骨折,7例胫骨/腓骨骨折)。术中使用3.5 mm锁定头受限接触-动态加压钢板(LH-LC-DCP)作为锁定模式,在每个近端和远端骨折处插入两枚3.5 mm(不同长度)的自攻锁定头皮质螺钉。骨板应用于桡骨、胫骨内侧面。术后x线片评估骨折复位和固定情况,所有病例均良好。定期随访显示骨折复位良好,内固定无改变。螺钉松动仅1例,无其他并发症。所有植入物在镇静下于术后第68天或之前取出。在重新评估的最后一天,所有动物的体重都很好,肢体功能接近正常。皮上骨结构在骨折部位提供了良好的复位和固定,类似于外部骨骼固定架,而不影响轴向和扭转刚度。
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引用次数: 0
Assessment of physiological responses and milk production in Jersey crossbred cows at different stratum of THI inside the cow barn 牛棚内不同THI层对泽西杂交奶牛生理反应及产奶量的影响
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-13 DOI: 10.56093/ijans.v93i9.119779
DILIP KUMAR MANDAL, SAROJ RAI, A CHATTERJEE, C BHAKAT, T K DUTTA, M K GHOSH
Environmental heat stress is one of the restrictive factors for optimum production of dairy cows. Present study was conducted to assess alteration in cardinal physiological responses, heat tolerance indices and milk yield in Jersey crossbred cows at three levels of thermal humidity index (THI), viz. THI-1 (<72), THI-2 (72-80) and THI-3 (>80). THI levels significantly influenced rectal temperature (RT) and respiration rate (RR), but not the pulse rate. With increase in THI level 1 to 3, the RT and RR increased significantly by 0.75°F and 10.70 counts/min during morning and 0.92°F and 12.5 counts/min during afternoon, respectively. The respective enhancement between THI-2 to THI-3 was 0.48°F and 7.06/min in morning and 0.58°F and 7.45/min in afternoon. Among the cardinal physiologicalresponses, breathing rate was the most diurnal variable parameter and it increased by 22-29% from 8:00 am to 2:00 pm with enhancement of stress levels fromTHI-1 to THI-3. Measures of heat tolerance indices revealed that cows were comfortable at THI-1 and discomfort levels were prominent at THI > 80. Low yielders (<10 kg/day) resisted natural heat stress by oriented decline in daily milk yield (DMY) and sustained DMY even at THI >80. In case of high yielders (>10 kg/day) increase in THI level 1 to 2, DMY did not drop significantly; in contrast, with increase of THI-2 to THI-3, the DMY declined. It was concluded that enhanced breathing rate was the most prominent bio-indicator of natural heat stress in Jersey crossbred cows and at THI>80 production decline due to natural heat stress was nonsignificant in low yielders, whereas highly significant in high yielders (more than 21%). It was recommended that atTHI >80, inside of cow shed needs immediate amelioration measures to diminish production losses and high yielders (>10 kg/day) warrant more attention than low yielders during heat stress.
环境热应激是制约奶牛最佳产量的因素之一。本试验研究了泽西杂交奶牛在3个热湿指数(THI -1 (<72)、THI-2(72-80)和THI-3 (>80)水平下的基本生理反应、耐热性指标和产奶量的变化。THI水平显著影响直肠温度(RT)和呼吸速率(RR),但不影响脉搏率。随着THI水平1 ~ 3的增加,RT和RR在上午分别显著增加0.75°F和10.70计数/min,在下午分别显著增加0.92°F和12.5计数/min。THI-2与THI-3在上午分别增强0.48°F和7.06/min,下午分别增强0.58°F和7.45/min。在主要生理反应中,呼吸速率是最具昼夜变化的参数,随着应激水平从thi -1到THI-3的增加,呼吸速率从上午8:00到下午2:00增加了22-29%。热耐受性指标的测量表明,奶牛在THI-1时舒适,而在THI >时不适程度突出;80. 低产奶量(10公斤/天)通过定向下降日产奶量(DMY)来抵抗自然热胁迫,即使在80公斤/天也能维持DMY。高产作物(10公斤/天)在THI水平1 ~ 2的情况下,DMY没有显著下降;而随着THI-2 ~ THI-3含量的增加,DMY呈下降趋势。由此可见,呼吸频率的增加是泽西杂交奶牛自然热应激最显著的生物指标,在thh >80时,低产奶牛因自然热应激导致的产量下降不显著,而高产奶牛因自然热应激导致的产量下降极显著(超过21%)。建议立即采取措施改善牛棚内的atTHI >80,以减少生产损失,在高温胁迫下,高产牛(> kg/d)比低产牛更值得关注。
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引用次数: 0
Effect of dietary supplementation of inulin with low and high concentrate diet on growth performance and nutrient digestibility in Barbari goats 低精料和高精料饲粮中添加菊粉对巴巴山羊生长性能和营养物质消化率的影响
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-13 DOI: 10.56093/ijans.v93i9.131433
ABHISHEK KUMAR, AHMAD FAHIM, AMIT KUMAR, D K SINGH, GULAB CHANDRA, NAZIM ALI, DEBASHIS ROY, ANUJ KUMAR
This study aimed to determine the effect of dietary supplementation of inulin on growth performance, feed conversion and nutrient digestibility in Barbari goats fed high and low concentrate diet for a period of 90 days. Twenty four yearling female Barbari goats were randomly divided into four equal groups (n=6) according to average body weight (18.24±1.16 kg). Treated animals in Group 1 were fed low concentrate diet (LCD; concentrate roughage ratio 40:60) and Group 2 (LCIN) with 2% inulin supplementation (% DM basis). Similarly, Group 3 was fed high concentrate diet (HCD; concentrate roughage ratio 60:40) and Group 4 (HCIN) with 2% inulinsupplementation. Results showed that average daily gain (ADG) and feed conversion efficiency (FCE) were higher in inulin supplemented groups than the non-supplemented group. There was no increase in mean live body weight (BW) and dry matter intake (DMI) with inulin supplementation either in LCD or HCD treatment groups.The mean body condition score (BCS) was also similar in all groups. However, faecal score (FS) was superior in inulin supplemented group observed after 30 days of feeding trial. There was significant improvement in the digestibility coefficient of crude protein and DCP intake. It was concluded that inulin supplementation either in high concentrate and low concentrate diet of growing goats resulted in improvement in growth performance, faecal score and nutrient digestibility.
本试验旨在研究饲粮中添加菊粉对饲喂高、低精料日粮90 d的巴巴山羊生长性能、饲料转化率和营养物质消化率的影响。选取24只1岁雌性巴巴利山羊,按平均体重(18.24±1.16 kg)随机分为4组(n=6)。1组饲喂低精料日粮(LCD;精料粗料比40:60)和添加2%菊粉(% DM基础)的第二组(LCIN)。同样,第3组饲喂高精料日粮(HCD;精料比60:40)和添加2%菊粉的第4组(HCIN)。结果表明:菊粉添加组的平均日增重(ADG)和饲料转化效率(FCE)均高于未添加组。添加菊粉后,LCD组和HCD组的平均活重(BW)和干物质采食量(DMI)均未增加。各组平均体况评分(BCS)也相似。饲喂试验30 d后观察,菊粉添加组的粪便评分(FS)明显高于对照组。饲粮中粗蛋白质消化系数和粗蛋白质消化系数均有显著提高。由此可见,在生长山羊高精料和低精料饲粮中添加菊粉均可提高生长性能、粪便评分和营养物质消化率。
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引用次数: 0
Herbal remedies for male infertility and spermatogenic activity in animals: A review 男性不育和动物生精活性的草药治疗:综述
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-13 DOI: 10.56093/ijans.v93i9.113326
CHETNA GANGWAR, GURURAJ KUMARESAN, ANIL KUMAR MISHRA, ASHOK KUMAR, R POUROUCHOTTAMANE, BHUVANESHWAR RAI
Male infertility is becoming an important untouched area that needs immediate attention due to the increasing demand for breeding strategies, keeping in view the production and increasing per animal productivity. Many additives and antioxidants have been tried for enhancing the seminal quality, but still there is no evidence of full- proof effect on the conception rates in female animals. However, herbal preparations which promise multi-factorial effect in the breeding male animals can be explored, and in turn could prove to be a better tool to encounter the problem of male infertility holistically. The herbal preparations and its effect at the cellular, molecular and metabolic level still needs to be understood. However, the advantage of using the herbal ingredients could be, use of available local herbal ingredients which are more economical, affordable, can reduce the use of hormonal therapy, have less side effects on long term usage, and have greater acceptability by the farmers. These herbal ingredients will be useful in breeding programmes for improvement of germplasm in terms of productivity. The current review covers how the herbs can be utilized in improving the semen quality and quantity, enhancing function of sertoli and leydig cells, mating behaviour, fecundity, seminal antioxidant status, hypophyseal adrenal gonadal axis cum endocrine regulation, microcirculation of testes, as well as in semen cryopreservation and post thaw quality of different species.
由于对育种策略的需求不断增加,雄性不育正在成为一个需要立即关注的重要未触及领域,保持对生产和提高每头动物生产力的关注。为了提高精子质量,人们尝试了许多添加剂和抗氧化剂,但还没有证据表明它们对雌性动物的受孕率有完全的影响。然而,草药制剂有望在繁殖雄性动物中发挥多因子作用,反过来可能被证明是一个更好的工具,以全面解决雄性不育问题。草药制剂及其在细胞、分子和代谢水平上的作用仍有待了解。然而,使用草药成分的优点可能是,使用当地可获得的草药成分,更经济,负担得起,可以减少激素治疗的使用,长期使用的副作用更小,并且更容易被农民接受。这些草药成分将有助于育种计划,以提高种质资源的生产力。本文综述了这些植物在改善不同物种精液质量和数量、增强精液的支持和leyleycells的功能、交配行为、生殖力、精子抗氧化状态、垂体、肾上腺、性腺轴和内分泌调节、睾丸微循环、精液冷冻和解冻后质量等方面的应用。
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引用次数: 0
Mapping the research publication trends among ICAR-Animal Sciences Research Institutes in India: Web of Science-based scientometric study 绘制印度icar -动物科学研究所的研究出版趋势:基于科学的科学计量学研究网络
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-13 DOI: 10.56093/ijans.v93i9.115679
P KRISHNAN, M HEMALATHA, SUREKHA AGARWAL, P GIREESH-BABU, B M NAVEENA, CH SRINIVASA RAO
In the current study we have undertaken the scientometric analysis to map research publications from 19 different animal science research institutions under the Indian Council of Agricultural Research (ICAR), for a period of two decades (1998 to 2020), using the Web of Science (WoS) platform of Clarivate Analytics. We analyzed different scientometric parameters like year-wise citation records, institute-wise publication output, authorship pattern, publication types, top collaborating countries, research areas and journals, and relative growth rate and doubling time. Current analysis revealed that the maximum number of research articles were published in 2017 and ICAR-Indian Veterinary Research Institute contributed to the highest number of research papers and citations during thestudy period. Multi-authorship papers were significantly higher than single and double-authored papers. The study showed that in the field of animal sciences, the USA is the top collaborator with India resulting in 417 collaborative research papers. The Indian Journal of Animal Sciences carried the maximum number of research papers published by researchers from ICAR-Animal Science Research Institutes (ASRI) during 1998-2020. Relative Growth Rate (RGR) showed a decreasing trend in research publications while the doubling time increased during this period. The paper provides a comprehensive account of the research trends in animal sciences research and also insights for strengthening research focus in India.
在当前的研究中,我们使用Clarivate Analytics的Web of science (WoS)平台,对印度农业研究委员会(ICAR)下属的19个不同动物科学研究机构的研究出版物进行了科学计量分析,绘制了20年(1998年至2020年)的地图。我们分析了不同的科学计量参数,如年度引文记录、研究所出版物产量、作者模式、出版物类型、主要合作国家、研究领域和期刊、相对增长率和翻倍时间。目前的分析显示,2017年发表的研究论文数量最多,icar -印度兽医研究所在研究期间贡献了最多的研究论文和引用。多作者论文显著高于单作者和双作者论文。该研究表明,在动物科学领域,美国是与印度合作最多的国家,共发表了417篇合作研究论文。《印度动物科学杂志》在1998-2020年期间刊登了icar -动物科学研究所(ASRI)研究人员发表的最多研究论文。研究出版物的相对增长率(RGR)在此期间呈下降趋势,而倍增时间呈增加趋势。该论文全面介绍了动物科学研究的研究趋势,并为加强印度的研究重点提供了见解。
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引用次数: 0
Comparative efficacy of certain extenders on preservation of liquid boar semen 几种膨化剂对公猪液体精液保存效果的比较
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-13 DOI: 10.56093/ijans.v93i9.116312
J KAUR, A K SINGH, S S DHINDSA, P SINGH, S K DAS
This study was carried out to compare the efficacy of certain liquid semen extenders for boar semen preservation. Semen ejaculates (n=30) from three boars (one ejaculate/boar/week) stationed at University pig farm, Ludhiana were collected using dummy sow and extended (1:4) in lactose-sodium salt of EDTA-Egg yolk (LSEEY); glucose sodium salt of EDTA-potassium sodium tartrate-sodium citrate (GEPS); and Modena (MOD). The extended semen was kept at 17°C up to 120 h in a BOD incubator. The semen was assessed for various sperm characteristics and lipid peroxidation at 0, 24, 48, 72, 96 and 120 h of preservation. The results revealed significantly higher percentage of sperm motility, viability, plasma membrane integrity and acrosome integrity in semen extended with MOD thanin GEPS and LSEEY at different preservation times. The mean percentage of in vitro capacitation/acrosome reacted spermatozoa was significantly higher in MOD as compared to GEPS and LSEEY at 96 h and 120 h of storage period. In all the extenders, the mean percentage of most sperm attributes decreased progressively as the storage period increased. Correspondingly, the MDA levels were lower in MOD than in their contemporary extenders at all hours of preservation. In conclusion, Modena exhibited improved sperm parameters and reduced oxidative stress for liquid preservation of boar semen.
本研究比较了几种液体精液填充剂对公猪精液的保存效果。采用假母猪采集卢迪亚那大学猪场3头公猪(1头/周)的射精精液(n=30),以1:4的比例浸泡在乳糖- edta -蛋黄钠盐(LSEEY)中;edta -酒石酸钠钾-柠檬酸钠葡萄糖钠盐;摩德纳(MOD)。延长后的精液在生化需氧量培养箱中于17°C保存至120 h。在保存0、24、48、72、96和120 h时评估精液的各种精子特征和脂质过氧化。结果表明,在不同保存时间,MOD延长的精液中精子活力、活力、质膜完整性和顶体完整性的百分比均显著高于GEPS和LSEEY。保存96 h和120 h时,MOD组体外获能/顶体反应精子的平均百分比显著高于GEPS和LSEEY组。在所有延长者中,大多数精子属性的平均百分比随着储存期的增加而逐渐降低。相应地,在保存的所有时间内,MOD的MDA水平都低于其同时代的扩展者。综上所述,摩德纳在液体保存公猪精液中表现出改善的精子参数和降低的氧化应激。
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引用次数: 0
Effect of dietary tannin and protein concentration on resistance and resilience of chokla lambs against Haemonchus contortus 饲粮单宁和蛋白质浓度对chokla羔羊对弯曲血蜱抗性和恢复力的影响
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-13 DOI: 10.56093/ijans.v93i9.122832
FAIZ AHMAD KHAN, ARTABANDHU SAHOO
Feeding trial was conducted for 7 weeks using 5-6 months old chokla lambs reared in helminth free conditions. The source of condensed tannin was dried leaves of khejri (Prosopis cineraria). The animals were randomly allocated to six treatment groups: (1) Low protein (LP, 10.0% CP) throughout the trial, (2) high protein (HP, 18.4% CP) throughout the trial, (3) LP (for initial 3 weeks of the experiment) and then combination of LP and Khejri (K), (4) combination of LP and K throughout the trial, (5) HP (for initial 3 weeks of the experiment) and then combination of HP and K, and (6) combination of HP and K throughout the trial. At day 0, all groups were trickle infected with infective H. contortus larvae and subsequently with 400-500 larvae/animal three times per week for 6 weeks. Therewas no improvement in resilience as well as resistance of lambs fed with combination of LP concentrate and khejri leaves. Lambs (4-5) were given salvage treatment in each group. Bottle jaw condition was seen in two lambs in each group. There was similar reduction in both PCV and Hb parameters in all the three groups fed with LP concentrate without any influence of inclusion of khejri leaves in the diet. Similarly CT inclusion did not influence the faecal egg count (FEC). On the other hand, improvement in both resistance and resilience was observed in lambs fed with combination of HP concentrate and khejri leaves. Inclusion of khejri leaves in HP concentrate fed lambs significantly affected both PCV and Hb. Faecal egg count was also significantly affected. There was significant effect on average daily gains (ADG). Voluntary feed intake was obtained in collective pens. Although there was reduction in feed intake in all the groups during week 2 to 3, it was more evident in lambs kept on LP concentrate. Therefore, it was suggested that when tannin was included with HP concentrate, an additive effect was seen which resulted in increase in protein availability to the lambs.
试验选用5 ~ 6月龄无蠕虫条件下饲养的chokla羔羊,饲喂7周。浓缩单宁的主要来源是木犀草(Prosopis cineraria)的干叶。将动物随机分为6个处理组:(1)全期低蛋白(LP, 10.0% CP)处理,(2)全期高蛋白(HP, 18.4% CP)处理,(3)全期低蛋白(试验前3周),然后LP与Khejri (K)联合处理,(4)全期LP与K联合处理,(5)全期HP(试验前3周),然后HP与K联合处理,(6)全期HP与K联合处理。在第0天,各组分别滴入侵染性弯纹夜蛾幼虫,然后每周3次滴入400 ~ 500只/只,连续6周。LP精料与海参叶配饲对羔羊的抗逆性和抗逆性均无显著提高。各组羔羊(4 ~ 5只)进行抢救处理。每组2只羔羊出现瓶颚病。在饲粮中不添加海参叶的情况下,饲喂LP精料的三组的PCV和Hb参数均有相似的降低。同样,CT检查不影响粪卵计数(FEC)。另一方面,HP精料与赫氏菌叶组合饲喂的羔羊的抵抗力和复原力均有提高。HP精饲羔羊中加入可氏菌叶对PCV和Hb均有显著影响。粪卵数也受到显著影响。对平均日增重(ADG)有显著影响。在集体围栏中自愿采食量。虽然在第2 ~ 3周,各组羔羊的采食量均有所减少,但以LP精料饲养的羔羊的采食量减少更为明显。由此可见,在HP精料中添加单宁可产生添加效应,提高羔羊的蛋白质利用率。
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引用次数: 0
Kinetics of humoral response to Pasteurella multocida in buffaloes against combined foot-and-mouth+haemorrhagic septicaemia vaccine 口蹄疫+出血性败血症联合疫苗对多杀性巴氏杆菌的体液反应动力学
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-09-13 DOI: 10.56093/ijans.v93i9.114913
NEENA RUSTAGI, NARESH KUMAR KAKKER, SUMAN SHEORAN, PATIL CHANDRASHEKHAR SANTOSH, SWATI DAHIYA, AKHIL KUMAR GUPTA
The present study reports kinetics of anti-P. multocida antibody (Ab) response at monthly intervals in Murrah buffaloes of different age groups vaccinated against combined Foot-and-mouth disease+haemorrhagic septicaemia (FMD+HS) vaccine. A total of 60 Murrah buffaloes of three age groups having 20 animals each: calves, heifers and adults were used to monitor anti-P. multocida Ab response at monthly intervals using single dilution indirect ELISA. The percentage of adult buffaloes protected were found to be the highest during all the six months post-vaccination followed by heifers and calves. The protective mean Ab titres were maintained up to six months post-vaccination for heifers and adults but not for calves. The F value (the ratio of two mean squares) for pre- and all the six month(s) post-vaccination and all the three age groups was significantly higher. Pearson Chi square value for pre-vaccination and all the six months except three months post-vaccination was significantly higher. Pearson correlation value was significantly higher with positive linear relationship. The data in the present study indicated that the combined FMD+HS vaccine was found to be effective in buffaloes of all age groups at government organised farm and could be an ideal approach in field conditions under Livestock Health Disease Control Program run by the Government of India.
本研究报道了抗p的动力学。不同年龄组Murrah水牛接种口蹄疫+出血性败血症(FMD+HS)联合疫苗后每个月的多毒抗体(Ab)应答共60头Murrah水牛,分为3个年龄组,每组20头:小牛、小母牛和成年水牛。用单次稀释间接ELISA检测每月一次的多毒抗体反应。在接种疫苗后的六个月内,受保护的成年水牛比例最高,其次是小母牛和小牛。接种疫苗后,小母牛和成年牛的保护性平均抗体滴度维持到6个月,但小牛则没有。接种前、接种后6个月及3个年龄组的F值(二均方差比)均显著升高。接种前和接种后6个月(除接种后3个月外)的Pearson卡方值均显著升高。Pearson相关值显著增高,呈正线性关系。本研究中的数据表明,在政府组织的农场中,口蹄疫+HS联合疫苗被发现对所有年龄组的水牛都有效,并且可能是印度政府管理的牲畜卫生疾病控制计划下野外条件下的理想方法。
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引用次数: 0
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Indian Journal of Animal Sciences
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