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Cryopreservation and freezability of epididymal and ejaculated stallion spermatozoa 种马附睾和射精精子的低温保存和冷冻性
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-02 DOI: 10.56093/ijans.v93i7.132542
TR TALLURI, DINESH JHAMB, NILENDU PAUL, S C MEHTA, JITENDAR SINGH, R K DEDAR, R A LEGHA, YASH PAL
The aim of the present study was to compare the seminal attributes and freezability of the epididymal sperm recovered from castrated stallions and ejaculated semen collected through artificial vagina from the same stallions previously. The seminal quality parameters viz., viability, sperm morphology, plasma membrane functional integrity, acrosome integrity and mitochondrial membrane potential were studied, and recorded immediately after fresh semen collection/retrieval, before freezing, and after cryopreservation. Similar protocols were followed for freezing the epididymal and ejaculated semen. From the current study, it was observed that the sperm concentration of the epididymal recovered was significantly higher than that of fresh ejaculation. Other seminal parameters in thefresh sample, like total and progressive sperm motility, sperm membrane functional integrity, acrosome integrity and kinematic parameters like VSL, STR, and linearity were similar without any significant differences between both. However, mitochondrial membrane potential, BCF and linearity of the spermatozoa differed significantly between the groups after cryopreservation. From the current study, it can be concluded that the protocols used for cryopreserving the fresh ejaculates can be adopted for freezing the stallion epididymal spermatozoa without causing significant damage and through this method; elite stallion germplasm can be safely cryopreserved and conserved.
本研究的目的是比较从阉割的种马中恢复的附睾精子和以前从同一种种马的人工阴道中收集的射精精液的精子属性和冷冻性。研究精子质量参数,即活力、精子形态、质膜功能完整性、顶体完整性和线粒体膜电位,并在新鲜精液采集/提取后、冷冻前和冷冻保存后立即记录。附睾和射精精液的冷冻也遵循类似的规程。从目前的研究中可以观察到,恢复附睾的精子浓度明显高于新鲜射精。新鲜样品中的其他精液参数,如精子总活力和进展活力、精子膜功能完整性、顶体完整性和运动学参数,如VSL、STR和线性度,两者之间没有显著差异。然而,冷冻保存后两组精子的线粒体膜电位、BCF和线性有显著差异。从目前的研究中可以得出结论,使用冷冻保存新鲜射精的方案可以对种马附睾精子进行冷冻,而不会造成明显的损害;优良种马种质可以安全超低温保存。
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引用次数: 0
Evaluation of forage qualities of sorghum (Sorghum bicolor L.) under varying jeevamrit formulations and their spraying interval 不同喷施配方及喷施间隔对高粱牧草品质的影响
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-02 DOI: 10.56093/ijans.v92i7.131922
MOHD. ARIF, RAVINDRA KUMAR, ARVIND KUMAR, D L GUPTA
An experiment was conducted at ICAR-Central Institute for Research on Goats, Makhdoom to generate precise information about different jeevamrit formulation and their spraying interval on forage quality of fodder sorghum during summer season of 2022. The treatments consisted of three jeevamrit formulations viz. jeevamrit-1 (5 kg cow dung + 2.5 litre cow urine), jeevamrit-2 (10 kg cow dung + 5 litre cow urine) and jeevamrit-3 (15 kg cow dung + 7.5 litre cow urine); and three spraying intervals viz. spraying at every one week’s interval, spraying at every two week’s interval and spraying at every three week’s interval. The experiment was laid out in factorial randomized block design with three replications. The results showed that among the different jeevamrit formulations, jeevamrit-3and among the different spraying intervals, spraying at every one week’s interval recorded maximum total dry matter yield, crude protein, ether extract, TDN content, dry matter intake, relative feed quality, net energy for lactation, nitrogen, phosphorus and potassium content in forage sorghum. However, among the different jeevamrit formulations, jeevamrit-2 and jeevamrit-3; and among the different spraying intervals, spraying of jeevamirt at every one week and every two week’s interval recorded at par values of all the nutritive parameters in fodder sorghum.
在icar - Makhdoom中央山羊研究所进行了一项试验,以获得2022年夏季不同jeevamrit配方及其喷施间隔对饲用高粱饲料质量的精确信息。处理包括三种jeevamrit配方,即jeevamrit-1(5公斤牛粪+ 2.5升牛尿),jeevamrit-2(10公斤牛粪+ 5升牛尿)和jeevamrit-3(15公斤牛粪+ 7.5升牛尿);三种喷施间隔,即每隔一周喷一次、每隔两周喷一次、每隔三周喷一次。试验采用因子随机区组设计,设3个重复。结果表明,在不同jeevamrit配方中,jeevamrit-3和不同喷施间隔中,每隔一周喷施一次,饲用高粱的总干物质产量、粗蛋白质、粗脂肪、TDN含量、干物质采食量、相对饲料品质、泌乳净能、氮、磷、钾含量最高。然而,在不同的jeevamrit配方中,jeevamrit-2和jeevamrit-3;在不同的施药间隔中,每隔一周和每隔两周施药一次,记录了饲用高粱各营养参数的平均值。
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引用次数: 0
Molecular characterization of toll-like receptor 2 gene in Bikaneri camels (Camelus dromedarius) 骆驼toll样受体2基因的分子特征
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-02 DOI: 10.56093/ijans.v92i7.125173
RAM KUMAR GAHLOT, BASANTI JYOTSANA, VED PRAKASH, SHIRISH DADARAO NARNAWARE, N S RATHORE
Toll-like receptors (TLRs) are an important family of receptors constituting the first line of defense system. Tolllike receptors play crucial roles in the innate immune system by recognizing pathogen-associated molecular patterns derived from various microbes. Present investigation was carried out to characterize the coding sequence of TLR2 gene in Indian dromedary camels. Blood was collected from five Bikaneri camels and genomic DNA was isolated. Eight pairs of overlapping primers were designed for amplification of TLR2 gene coding region. All the fragments were successfully amplified using Polymerase chain reaction (PCR). The PCR products were purified and subjected to Sanger’s sequencing. TLR2 gene fragment of 3213 bp consisting of 2287 bp of coding sequence of exon 2 and 926 bp of 3’untranslated region (UTR) was successfully amplified. The sequence generated was submitted to the NCBI with accession number MW557315.1. The encoded amino acids codes for 12 leucine rich repeats (LRR) (Accession no. UBN09134.1). At the nucleotide level, the Bikaneri camels’ TLR2 gene showed highest similarity with camelids (99.30 to 99.59%) followed by vicugna pacos (97.54%). The nucleotide sequence similarity was lowest with Homo sapiens (82.37%). The amino acid sequence based phylogenetic tree revealed close relationship between camelid species followed by pig (86.2%), whereas human, gorilla, horse and donkey were distantly placed on the tree.
toll样受体(TLRs)是一个重要的受体家族,构成了第一道防线。toll样受体通过识别来自各种微生物的病原体相关分子模式,在先天免疫系统中起着至关重要的作用。本文对印度单峰骆驼TLR2基因的编码序列进行了研究。采集了5只Bikaneri骆驼的血液,并分离了基因组DNA。设计了8对重叠引物,用于扩增TLR2基因编码区。所有片段均经聚合酶链反应(PCR)扩增成功。PCR产物纯化后进行Sanger测序。成功扩增出全长3213 bp的TLR2基因片段,其中包括2287 bp的外显子编码序列和926 bp的3 '非翻译区(UTR)。生成的序列提交给NCBI,登记号为MW557315.1。所编码的氨基酸编码12个富含亮氨酸的重复序列(LRR)。UBN09134.1)。在核苷酸水平上,骆驼TLR2基因与骆驼类相似性最高(99.30 ~ 99.59%),其次是骆马(97.54%)。与智人核苷酸序列相似性最低(82.37%)。基于氨基酸序列的系统进化树显示,骆驼与猪的亲缘关系较近(86.2%),人类、大猩猩、马和驴的亲缘关系较远。
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引用次数: 0
Systematic review and meta-analysis on brucellosis seroprevalence in ruminants (1970 – 2020) of India 1970 - 2020年印度反刍动物布鲁氏菌病血清流行率的系统回顾和荟萃分析
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-08-02 DOI: 10.56093/ijans.v92i7.132586
MICHAEL V LALRINZUALA, VINODHKUMAR O R, SHIKHA TAMTA, D K SINGH, C LALSANGZUALA, DAMINI KHAWASKAR, None ROTLUANGKIMI, B R SINGH, D K SINHA
Brucellosis is one of the most common contagious and neglected bacterial zoonotic diseases. The disease has been reported in ruminants in almost all Indian states. But there is a lack of comprehensive information on the seroprevalence of brucellosis in the Indian subcontinent. A systematic review and meta-analysis on the seroprevalence of brucellosis among ruminants of India was conducted from the published articles (January 1970 - June 2020) by including 172 studies screened from 567 publications. The estimated brucellosis seroprevalence of cattle, buffalo, sheep, goat, yak and mithun was 14% (95% CI: 12% - 16%), 8% (95% CI: 6% - 9%), 8% (95% CI: 7% - 10%), 8% (95% CI: 7% - 9%), 16% (95% CI: 7% - 28%) and 26% (95% CI: 12% - 42%), respectively. Sub-group analysiswas performed based on diagnostic tests, regions, publication year, and sample size. The estimated seroprevalence of brucellosis in cattle and goats was found to be higher in the central region compared to other regions. Similarly, the western region showed a higher seroprevalence for brucellosis in buffalo and sheep. Given the estimated animal population of 2021, the meta-analysis estimated that the total number of seropositive animals would be 26.95 million cattle (95% CI: 23.09–30.78), 8.78 million buffaloes (95% CI: 6.59–9.89), 5.94 million sheep (95% CI: 5.20–7.43), 11.91 million goats (95% CI: 10.42–13.40), 9.6 thousand yaks (95% CI: 0.0042–0.0168), and 100 thousand mithun (95% CI: 0.05–0.16). Further, the comprehensive picture of the brucellosis seroprevalence may help the decisionmaking authorities in formulating better prevention and control strategies for brucellosis in India.
布鲁氏菌病是最常见的传染性和被忽视的细菌性人畜共患疾病之一。印度几乎所有邦的反刍动物都报告了这种疾病。但是,缺乏关于印度次大陆布鲁氏菌病血清流行率的全面信息。从已发表的文章(1970年1月至2020年6月)中纳入从567份出版物中筛选的172项研究,对印度反刍动物布鲁氏菌病血清流行率进行了系统回顾和荟萃分析。估计牛、水牛、绵羊、山羊、牦牛和米顿的布鲁氏菌病血清患病率分别为14% (95% CI: 12% - 16%)、8% (95% CI: 6% - 9%)、8% (95% CI: 7% - 10%)、8% (95% CI: 7% - 9%)、16% (95% CI: 7% - 28%)和26% (95% CI: 12% - 42%)。根据诊断试验、地区、出版年份和样本量进行亚组分析。发现中部地区牛和山羊中布鲁氏菌病的估计血清流行率高于其他地区。同样,西部地区水牛和绵羊的布鲁氏菌病血清流行率较高。根据估计的2021年动物种群,meta分析估计血清阳性动物总数为2695万头牛(95% CI: 23.09-30.78)、878万头水牛(95% CI: 6.59-9.89)、594万头绵羊(95% CI: 5.20-7.43)、1191万头山羊(95% CI: 10.42-13.40)、960万头牦牛(95% CI: 0.0042-0.0168)和10万头米图翁(95% CI: 0.05-0.16)。此外,布鲁氏菌病血清患病率的全面情况可能有助于决策当局在印度制定更好的布鲁氏菌病预防和控制战略。
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引用次数: 0
Sources of risks in livestock production and their management strategies in northern India 印度北部畜牧业生产的风险来源及其管理战略
4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2023-01-03 DOI: 10.56093/ijans.v88i5.80012
SUBHASH CHAND, PREM NARAYAN, KHYALI R CHAUDHARY
This study was undertaken to analyze the opinion on risks sources and their management strategies in livestock enterprises. Data collected through a field survey conducted in Haryana and Rajasthan in 2015–16 from 913 livestock farmers on structured schedule forms the basis of this paper. Rajasthan depicts dry and harsh tract and Haryana with assured irrigated, and both contribute significant share in milk production. The socio-economic and demographic factors did not vary significantly except milk yield, experience in dairy, annual income for different categories of farmers. Our observation indicated that livestock owner faced risks from different sources, viz. breeding, animal health, marketing, institutional and policy related. Further risk management strategies including better breed, animal health checkup, seeking advice of veterinary expert, friend consultations, forward selling contracts, maintenance of farm inventory, accessibility to different financial, extension and government institutions etc. were adopted to cope up with risks in livestock enterprise. The determinants of source of risks were significantly influenced by a number of socio-economic and demographic characteristics. The herd size had negative relation with extent of various risks. Rajasthan farmers face higher degree of risk than Haryana. Higher education status also indicated that the lover risks and other factors like knowledge level and milk yield indicated positive influence of source of risks means higher knowledge level and yield, reduce the risks. The policy makers and researchers should see the solutions and options to minimize the source of risks in livestock production and management.
本研究旨在分析畜牧业企业对风险来源及管理策略的看法。2015年在哈里亚纳邦和拉贾斯坦邦进行的实地调查收集了913名畜牧农民的数据,这些数据是根据结构化时间表收集的。拉贾斯坦邦描绘了干旱和严酷的土地,哈里亚纳邦有保证的灌溉,两者都在牛奶生产中占有重要份额。除了产奶量、奶业经验、不同类别农民的年收入外,社会经济和人口因素没有显著差异。我们的观察表明,畜禽所有者面临着来自不同来源的风险,即育种、动物卫生、营销、制度和政策相关的风险。进一步的风险管理策略包括优良品种、动物健康检查、寻求兽医专家建议、朋友咨询、远期销售合同、维持农场库存、获得不同的金融、推广和政府机构等,以应对畜牧企业的风险。风险来源的决定因素受到一些社会经济和人口特征的重大影响。畜群规模与各种风险程度呈负相关。拉贾斯坦邦农民面临的风险高于哈里亚纳邦。高等教育程度也表明情人风险与其他因素如知识水平和产奶量呈正相关,风险来源意味着更高的知识水平和产奶量,降低风险。决策者和研究人员应该看到在畜牧生产和管理中最大限度地减少风险源的解决办法和选择方案。
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引用次数: 6
Effect of preputial washing and antibiotic treatment on bacterial load and preservability of frozen bovine semen 包皮清洗及抗生素处理对冷冻牛精液细菌载量及保鲜性的影响
IF 0.4 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2019-08-06 DOI: 10.31220/osf.io/5mbnt
C. Bhakat, V. S. Raina
This research is about effect of preputial washing and antibiotic treatment on bacterial load and preservability of frozen bovine semen
本研究是关于包皮冲洗和抗生素处理对冷冻牛精液细菌载量和保存性的影响
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引用次数: 7
Growth characteristics, economics and hair mineral status of camel calves reared in different system of management 不同管理体制下饲养骆驼犊牛的生长特性、经济性及毛矿物状况
IF 0.4 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2019-08-05 DOI: 10.31219/osf.io/hnpsq
C. Bhakat, N. Saini, K. Pathak
This research is about growth characteristics, economics and hair mineral status of camel calves reared in different system of management and Camel management, Camelus dromedaries, Farmers, Milk, Desert eco-systems, Climate change, Camel use,
本研究是关于不同管理系统和骆驼管理系统、骆驼、农民、牛奶、沙漠生态系统、气候变化、骆驼的使用、,
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引用次数: 6
Important factors affection sustainable livelihood of camel dairying on changing scenario of desert ecosystem 沙漠生态系统变化情景下影响骆驼养殖业可持续生计的重要因素
IF 0.4 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2019-08-05 DOI: 10.31219/osf.io/tmv8n
C. Bhakat, K. Pathak
This research is about important factors affection sustainable livelihood of camel dairying on changing scenario of desert ecosystem and Camel management, Camelus dromedaries, Farmers, Milk, Desert eco-systems, Climate change, Camel use
研究了沙漠生态系统变化情景及骆驼管理、单峰骆驼、农民、奶、沙漠生态系统、气候变化、骆驼利用等影响骆驼可持续生计的重要因素
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引用次数: 1
Influence of FecB gene in greasy fleece yield in Malpura, Garole and their crossbreeds FecB基因对马尔普拉、加洛尔及其杂交品种油羊毛产量的影响
IF 0.4 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 2016-12-01 DOI: 10.15740/HAS/TAJAS/11.2/171-172
K. Patil, B. R. Ulmek, A. L. Arora
FecB gene played important role in GFY. However improvement in GM crosses with regards to growth traits was very slow as compared to the Malpura. Therefore back crossing of GM crosses with Malpura ram or ewe might be useful for improving production and reproduction traits.
FecB基因在GFY中起重要作用。然而,与马尔普拉相比,转基因杂交在生长性状方面的改善非常缓慢。因此,将转基因杂种与马尔普拉公羊或母羊进行回交,可能有助于提高生产和繁殖性状。
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引用次数: 0
Laboratory Production of Cattle Embryos. 牛胚胎的实验室生产。
IF 0.4 4区 农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE Pub Date : 1997-01-01 DOI: 10.1079/9780851996660.0000
S. Guraya
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引用次数: 17
期刊
Indian Journal of Animal Sciences
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