Pub Date : 2023-09-13DOI: 10.56093/ijans.v93i9.129508
J D MOHANTY, B PANIGRAHI, N PANDA, J BAGH, L SAMAL
A study was conducted to evaluate the effect of feeding concentrate to suckling lambs on their performance. Experiment comprised 40 day old suckling lambs having 1.56 kg average birth weight, with 20 female and 20 male lambs selected randomly from the flocks of four farmers in village Mundida, Odisha. The male and female lambs were divided into two experimental groups so that each experimental group of 20 lambs consisted of 10 male and equal numbers of females. Group 1 (Control) was maintained on suckling and grazing only, while Group 2, balanced concentrate mixture was supplemented in addition to suckling and grazing. Concentrate was supplemented from 2nd fortnight upto 6th fortnight of age. Body weight, average daily gain were recorded upto14th fortnight ofage. Body measurements were recorded at every fortnight interval. Either of the male or female lambs group body weight did not differ significantly till the 2nd fortnight, while upon concentrate supplementation from 2nd fortnight, the lambs in Group 2 showed significantly higher body weights and average daily gain from 3rd fortnight till the end of observation at every stage (14th fortnight). Body measurements differences between the groups were observed only after commencement of the concentrate supplementation which resulted in higher values for the lambs in Group 2 from 4th or 5th till the last fortnight. Results revealed that supplementation of concentrate to the four weeks old grazing lambs for a period of 8 weeks improved the body weight, weight gain and reduces cost of lamb production.
{"title":"Effect of supplementary concentrate feeding to lambs on the growth performance and body morphometry","authors":"J D MOHANTY, B PANIGRAHI, N PANDA, J BAGH, L SAMAL","doi":"10.56093/ijans.v93i9.129508","DOIUrl":"https://doi.org/10.56093/ijans.v93i9.129508","url":null,"abstract":"A study was conducted to evaluate the effect of feeding concentrate to suckling lambs on their performance. Experiment comprised 40 day old suckling lambs having 1.56 kg average birth weight, with 20 female and 20 male lambs selected randomly from the flocks of four farmers in village Mundida, Odisha. The male and female lambs were divided into two experimental groups so that each experimental group of 20 lambs consisted of 10 male and equal numbers of females. Group 1 (Control) was maintained on suckling and grazing only, while Group 2, balanced concentrate mixture was supplemented in addition to suckling and grazing. Concentrate was supplemented from 2nd fortnight upto 6th fortnight of age. Body weight, average daily gain were recorded upto14th fortnight ofage. Body measurements were recorded at every fortnight interval. Either of the male or female lambs group body weight did not differ significantly till the 2nd fortnight, while upon concentrate supplementation from 2nd fortnight, the lambs in Group 2 showed significantly higher body weights and average daily gain from 3rd fortnight till the end of observation at every stage (14th fortnight). Body measurements differences between the groups were observed only after commencement of the concentrate supplementation which resulted in higher values for the lambs in Group 2 from 4th or 5th till the last fortnight. Results revealed that supplementation of concentrate to the four weeks old grazing lambs for a period of 8 weeks improved the body weight, weight gain and reduces cost of lamb production.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135740304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-13DOI: 10.56093/ijans.v93i9.131419
None ANIL, T K DUTTA, A CHATTERJEE, SUSHIL KUMAR YADAV, D K MANDAL, A MOHAMMAD
The present study was carried out to evaluate the effect of supplementation of exogenous fibrolytic enzymes (EFE) on voluntary feed intake, availability of different nutrients and growth performance in weaned crossbred calves. Fifteen weaned healthy female Jersey crossbred calves (weight 79.73±3.46 kg; age 234.5±11.33 days) were divided equally into three groups viz., Control (T0), Treatment-1 (T1) and Treatment-2 (T2). Growth trial was conducted on these calves for 90 days. Animals in each treatment group were fed ad lib. total mixed ration (TMR) (CP 12%, TDN 65%) individually for 90 days. Calves under control (T0) group were fed ad lib. TMR (without EFE). Calves under T1 and T2 were fed ad lib. TMR supplemented with EFE cellulase and xylanase @ 8000 and 16000 IU/kg DM of TMR and with EFE cellulase and xylanase @ 12000 and 24000 IU/kg DM of TMR, respectively. Significantly greater DMI per unit body weight (kg/100 kg BW and g/kg W0.75) was recorded in enzyme supplemented groups compared to control group; however, difference between T1 and T2 was non-significant. Addition of EFE in the TMR diet of calves increased TDN and DCP intake (g/kg W0.75) in T1 and T2 than T0 . Supplementation of EFE also enhanced TDN (%), DCP (%), DNDF (%), DE (Mcal/kg of feed) and ME (Mcal/kg of feed) value of the diets under T1 and T2 than T0. Average daily weight gain (g/d/calf) was estimated greater in T1 and T2 than T0. The feed conversion efficiency was similar among three treatments. Whereas, plasma total protein and albumin concentration increased significantly due to supplementation of EFE in the diet of calves. Therefore, it may be concluded that supplementation of exogenous fibrolytic enzymes (cellulase and xylanase @ 8000 and 16000 IU/kg TMR DM) may boost growth potential of finisher Jersey crossbred calves with enhanced intake of nutrients.
{"title":"Effect of exogenous fibrolytic enzymes supplementation to improve voluntary intake, availability of nutrients and growth performance in weaned crossbred calves","authors":"None ANIL, T K DUTTA, A CHATTERJEE, SUSHIL KUMAR YADAV, D K MANDAL, A MOHAMMAD","doi":"10.56093/ijans.v93i9.131419","DOIUrl":"https://doi.org/10.56093/ijans.v93i9.131419","url":null,"abstract":"The present study was carried out to evaluate the effect of supplementation of exogenous fibrolytic enzymes (EFE) on voluntary feed intake, availability of different nutrients and growth performance in weaned crossbred calves. Fifteen weaned healthy female Jersey crossbred calves (weight 79.73±3.46 kg; age 234.5±11.33 days) were divided equally into three groups viz., Control (T0), Treatment-1 (T1) and Treatment-2 (T2). Growth trial was conducted on these calves for 90 days. Animals in each treatment group were fed ad lib. total mixed ration (TMR) (CP 12%, TDN 65%) individually for 90 days. Calves under control (T0) group were fed ad lib. TMR (without EFE). Calves under T1 and T2 were fed ad lib. TMR supplemented with EFE cellulase and xylanase @ 8000 and 16000 IU/kg DM of TMR and with EFE cellulase and xylanase @ 12000 and 24000 IU/kg DM of TMR, respectively. Significantly greater DMI per unit body weight (kg/100 kg BW and g/kg W0.75) was recorded in enzyme supplemented groups compared to control group; however, difference between T1 and T2 was non-significant. Addition of EFE in the TMR diet of calves increased TDN and DCP intake (g/kg W0.75) in T1 and T2 than T0 . Supplementation of EFE also enhanced TDN (%), DCP (%), DNDF (%), DE (Mcal/kg of feed) and ME (Mcal/kg of feed) value of the diets under T1 and T2 than T0. Average daily weight gain (g/d/calf) was estimated greater in T1 and T2 than T0. The feed conversion efficiency was similar among three treatments. Whereas, plasma total protein and albumin concentration increased significantly due to supplementation of EFE in the diet of calves. Therefore, it may be concluded that supplementation of exogenous fibrolytic enzymes (cellulase and xylanase @ 8000 and 16000 IU/kg TMR DM) may boost growth potential of finisher Jersey crossbred calves with enhanced intake of nutrients.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135740295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was carried out to detect mastitis in cattle milk samples and sensitive antibiotics for therapeutic uses. Milk samples from cattle (n = 1649) were tested for detection of mastitis by white side test (n = 1314) and culture examination (n = 335). Overall, 79.8% and 96.72% milk samples were positive by white side test and culture examination, respectively. Clinical mastitis and sub clinical mastitis were detected in 16.55% and 83.44% samples, respectively on the basis of presence of any clinical signs in milk or udder of animal. Gram-positive and gramnegative organisms were detected in 99.38% and 45.34% of the samples, respectively. Mixed infection of both was detected in 38.88% samples. More than 74% of both gram-positive and gram-negative isolates were found sensitiveagainst enrofloxacin and chloramphenicol followed by amikacin (69.44%), gentamicin (68.73%), moxifloxacin (67.87%) and levofloxacin (61.73%). Organisms were detected as multidrug resistant (MDR) organisms depending upon their MAR index value.
{"title":"Antibiogram of bacterial pathogens causing mastitis among cattle","authors":"RAHUL YADAV, PANKAJ KUMAR, ANAND PRAKASH, VANDNA BHANOT, PAWANJIT SINGH CHEEMA, NARESH JINDAL","doi":"10.56093/ijans.v93i9.127834","DOIUrl":"https://doi.org/10.56093/ijans.v93i9.127834","url":null,"abstract":"The present study was carried out to detect mastitis in cattle milk samples and sensitive antibiotics for therapeutic uses. Milk samples from cattle (n = 1649) were tested for detection of mastitis by white side test (n = 1314) and culture examination (n = 335). Overall, 79.8% and 96.72% milk samples were positive by white side test and culture examination, respectively. Clinical mastitis and sub clinical mastitis were detected in 16.55% and 83.44% samples, respectively on the basis of presence of any clinical signs in milk or udder of animal. Gram-positive and gramnegative organisms were detected in 99.38% and 45.34% of the samples, respectively. Mixed infection of both was detected in 38.88% samples. More than 74% of both gram-positive and gram-negative isolates were found sensitiveagainst enrofloxacin and chloramphenicol followed by amikacin (69.44%), gentamicin (68.73%), moxifloxacin (67.87%) and levofloxacin (61.73%). Organisms were detected as multidrug resistant (MDR) organisms depending upon their MAR index value.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"361 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135740432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-13DOI: 10.56093/ijans.v93i9.134732
PALLAVI UPADHYAY, M PALANIVELRAJAN, A SENTHILKUMAR, P SELVARAJ
There is limited data on minimally invasive coelioscopy in smaller exotic birds like budgerigar. This study was taken primarily to collect baseline data of internal organs of budgerigar during a diagnostic coelioscopic procedure. A 2.7 mm 180 mm rigid endoscope (Karl Storz) was used for the procedure where in lung, liver, heart, kidney, gonad and intestines were visualized and evaluated. Special emphasis is placed on assessing feasibility and safety of applying advanced diagnostic procedures in small birds.
在像虎皮鹦鹉这样的小型外来鸟类中进行微创腔镜检查的数据有限。本研究主要是在诊断性肠镜检查过程中收集虎皮鹦鹉内部器官的基线数据。使用2.7 mm 180 mm刚性内窥镜(Karl Storz)对肺、肝、心、肾、性腺和肠进行观察和评估。特别强调的是评估在小型鸟类中应用先进诊断程序的可行性和安全性。
{"title":"Diagnostic coelioscopy in a budgerigar (Melopsittacus undulatus) for evaluation of internal organs","authors":"PALLAVI UPADHYAY, M PALANIVELRAJAN, A SENTHILKUMAR, P SELVARAJ","doi":"10.56093/ijans.v93i9.134732","DOIUrl":"https://doi.org/10.56093/ijans.v93i9.134732","url":null,"abstract":"There is limited data on minimally invasive coelioscopy in smaller exotic birds like budgerigar. This study was taken primarily to collect baseline data of internal organs of budgerigar during a diagnostic coelioscopic procedure. A 2.7 mm 180 mm rigid endoscope (Karl Storz) was used for the procedure where in lung, liver, heart, kidney, gonad and intestines were visualized and evaluated. Special emphasis is placed on assessing feasibility and safety of applying advanced diagnostic procedures in small birds.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135740299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-13DOI: 10.56093/ijans.v93i9.131602
PRAVIN M LUNAGARIYA, SHAILESH V SHAH, YOGESH G` PATEL, JIGAR H PATEL
The experiment was designed to assess the effect of supplemental feeding of 40 g/day rumen-protected choline (33.5% w/w) in the total mixed ration of lactating cows on nutrient intake, lactation, reproduction, feed efficiency, and feed cost during 294 days of the lactation. The study was conducted at Livestock Research Station, Anand during 2018 to 2021. A total of sixteen cows were randomly distributed into two groups of eight each based on descending order of previous standard lactation yield and lactation numbers. An intake and milk yield were calculated for 1-21 biweekly periods. The milk composition was assessed four times during lactation. The body condition score (BCS) on a 1-5 point scale with an increment of 0.25 points was measured at -21, -14, -7, 0, 7, 14, 21, 35, 49, 63, 91, and 119 days of calving. The supplemental feeding of rumen-protected choline (RPC) in T2 group resulted in lower nutrient intake, lesser insemination to conceive, and service period with higher 4% fat corrected milk (FCM) yield in dairy cows. The body condition score and milk yield persistency were higher along with improved feed efficiency and feed economics in the T2 group as compared to the T1 group. The daily feeding of 40 g RPC during -21 to 120 days of calving resulted in significantly lower nutrient intake with improved 4% FCM yield, reproductive performance, feed efficiency, and feed economics in dairy cows.
{"title":"Lactation performance of dairy cows on feeding rumen-protected choline","authors":"PRAVIN M LUNAGARIYA, SHAILESH V SHAH, YOGESH G` PATEL, JIGAR H PATEL","doi":"10.56093/ijans.v93i9.131602","DOIUrl":"https://doi.org/10.56093/ijans.v93i9.131602","url":null,"abstract":"The experiment was designed to assess the effect of supplemental feeding of 40 g/day rumen-protected choline (33.5% w/w) in the total mixed ration of lactating cows on nutrient intake, lactation, reproduction, feed efficiency, and feed cost during 294 days of the lactation. The study was conducted at Livestock Research Station, Anand during 2018 to 2021. A total of sixteen cows were randomly distributed into two groups of eight each based on descending order of previous standard lactation yield and lactation numbers. An intake and milk yield were calculated for 1-21 biweekly periods. The milk composition was assessed four times during lactation. The body condition score (BCS) on a 1-5 point scale with an increment of 0.25 points was measured at -21, -14, -7, 0, 7, 14, 21, 35, 49, 63, 91, and 119 days of calving. The supplemental feeding of rumen-protected choline (RPC) in T2 group resulted in lower nutrient intake, lesser insemination to conceive, and service period with higher 4% fat corrected milk (FCM) yield in dairy cows. The body condition score and milk yield persistency were higher along with improved feed efficiency and feed economics in the T2 group as compared to the T1 group. The daily feeding of 40 g RPC during -21 to 120 days of calving resulted in significantly lower nutrient intake with improved 4% FCM yield, reproductive performance, feed efficiency, and feed economics in dairy cows.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135781781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was conducted to evaluate resistance against ionophores (salinomycin and maduramicin) in four field isolates (F) of Eimeria tenella collected from four distantly located poultry farms from Union territory of Jammu and Kashmir. The purity of isolates was ascertained by COCCIMORPH and ITS-1 gene amplification for E. tenella. For the drug sensitivity trial, Cobb strain, day-old male broiler chicks were used. At the age of 10 days, the birds were transferred ad random in 13 groups to battery cage system. All birds, except non infected non-medicated control were infected with 105 sporulated oocysts each at two weeks. Prophylactic medication with ionophores commenced two days prior to infection in eight groups (two medicated groups for each of the four isolates). Another four groups served as infected and non-medicated control. Seven days post infection, all the surviving birds were sacrificed to study drug resistance by assessing the global index for individual ionophore compounds. The F-1 exhibited resistance against salinomycin and maduramicin whereas, the F-2 showed comparatively improved global index for both salinomycin and maduramicin thus indicating limited efficacy. The F-3 exhibited partial resistance against salinomycin and limited efficacy in case of maduaramicin, and the F-4 was observed to be resistant against salinomycin whereas partially resistant against maduramicin. The present study provides evidence of resistance against maduramicin and salinomycin in field isolates of E. tenella from Union Territory of Jammu and Kashmir which may aid in management of poultry coccidiosis.
{"title":"Evaluation of ionophore resistance in field isolates of Eimeria tenella from Jammu and Kashmir","authors":"AIMAN KHURSHEED, ANISH YADAV, VIKAS YADAV, OMER M SOFI, ANAND KUSHWAHA, SHAFIYA I RAFIQI, RAJESH GODARA, SHILPA SOOD, DIBYENDU CHAKRABORTY, RAJESH KATOCH","doi":"10.56093/ijans.v93i9.132460","DOIUrl":"https://doi.org/10.56093/ijans.v93i9.132460","url":null,"abstract":"The present study was conducted to evaluate resistance against ionophores (salinomycin and maduramicin) in four field isolates (F) of Eimeria tenella collected from four distantly located poultry farms from Union territory of Jammu and Kashmir. The purity of isolates was ascertained by COCCIMORPH and ITS-1 gene amplification for E. tenella. For the drug sensitivity trial, Cobb strain, day-old male broiler chicks were used. At the age of 10 days, the birds were transferred ad random in 13 groups to battery cage system. All birds, except non infected non-medicated control were infected with 105 sporulated oocysts each at two weeks. Prophylactic medication with ionophores commenced two days prior to infection in eight groups (two medicated groups for each of the four isolates). Another four groups served as infected and non-medicated control. Seven days post infection, all the surviving birds were sacrificed to study drug resistance by assessing the global index for individual ionophore compounds. The F-1 exhibited resistance against salinomycin and maduramicin whereas, the F-2 showed comparatively improved global index for both salinomycin and maduramicin thus indicating limited efficacy. The F-3 exhibited partial resistance against salinomycin and limited efficacy in case of maduaramicin, and the F-4 was observed to be resistant against salinomycin whereas partially resistant against maduramicin. The present study provides evidence of resistance against maduramicin and salinomycin in field isolates of E. tenella from Union Territory of Jammu and Kashmir which may aid in management of poultry coccidiosis.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135740434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-13DOI: 10.56093/ijans.v93i9.129435
QAZI SHEHRIYAR SAHIB, HAIDER ALI AHMED, ABDUL MAJEED GANAI, JAVID FAROOQ, GOWHER GULL SHEIKH, ISLAM-UD-DIN SHEIKH, YASIR AFZAL BEIGH
The present experiment was conducted to evaluate the palatability, growth and economics of the silkworm pupae meal (SWPM) feeding, replacing fish meal (FM) in cattle calves. Crossbred cattle calves (15) around one-month of age were selected for the study and were divided into three equal groups (n=5) viz., T0 (100% FM), T1 (75% FM: 25% SWPM) and T2 (50% FM: 50% SWPM). To estimate the growth parameters fortnightly, a growth trial of 90 days was conducted, and a digestibility trial of 7 days was conducted at the end of experiment. The results revealed non-significant differences between the experimental groups in DMI, OMI, periodical body weight, body weight gain, average daily gain (ADG) (g/d), feed conversion ratio (FCR), feed conversion efficiency (FCE) (%), digestibility coefficients of dry matter (DM), organic matter (OM) and crude protein (CP). Significant differences were observed in digestibility coefficients of crude fibre (CF), ether extract (EE) and nitrogen free extract (NFE) between the groups. The digestible nutrients intake revealed significant difference only in CPI (g/d) with higher values in T0 followed by T1 and T2, but non-significant differences were observed in DDMI, DCPI and TDNI. The economics of feeding showed a decrease in the feed cost and feeding cost per kg body weight gain with increased inclusion of SWPM. Thus, it is concluded that costly FM can be replaced upto 50% level by SWPM without anyadverse effect on feed intake, growth and nutrient utilization of crossbred cattle calves.
{"title":"Evaluation of silkworm pupae meal based calf starter diet on the performance of crossbred cattle calves","authors":"QAZI SHEHRIYAR SAHIB, HAIDER ALI AHMED, ABDUL MAJEED GANAI, JAVID FAROOQ, GOWHER GULL SHEIKH, ISLAM-UD-DIN SHEIKH, YASIR AFZAL BEIGH","doi":"10.56093/ijans.v93i9.129435","DOIUrl":"https://doi.org/10.56093/ijans.v93i9.129435","url":null,"abstract":"The present experiment was conducted to evaluate the palatability, growth and economics of the silkworm pupae meal (SWPM) feeding, replacing fish meal (FM) in cattle calves. Crossbred cattle calves (15) around one-month of age were selected for the study and were divided into three equal groups (n=5) viz., T0 (100% FM), T1 (75% FM: 25% SWPM) and T2 (50% FM: 50% SWPM). To estimate the growth parameters fortnightly, a growth trial of 90 days was conducted, and a digestibility trial of 7 days was conducted at the end of experiment. The results revealed non-significant differences between the experimental groups in DMI, OMI, periodical body weight, body weight gain, average daily gain (ADG) (g/d), feed conversion ratio (FCR), feed conversion efficiency (FCE) (%), digestibility coefficients of dry matter (DM), organic matter (OM) and crude protein (CP). Significant differences were observed in digestibility coefficients of crude fibre (CF), ether extract (EE) and nitrogen free extract (NFE) between the groups. The digestible nutrients intake revealed significant difference only in CPI (g/d) with higher values in T0 followed by T1 and T2, but non-significant differences were observed in DDMI, DCPI and TDNI. The economics of feeding showed a decrease in the feed cost and feeding cost per kg body weight gain with increased inclusion of SWPM. Thus, it is concluded that costly FM can be replaced upto 50% level by SWPM without anyadverse effect on feed intake, growth and nutrient utilization of crossbred cattle calves.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135740446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-13DOI: 10.56093/ijans.v93i9.110402
OVEAS RAFFIQ PARRAY, None MUHEET, RIYAZ AHMAD BHAT, HAMID ULLAH MALIK, SABIA QURESHI, SHAFAYAT AHMAD BEIG, QURAT UL AIN NAZIR, MAJID SHAFI KAWOOSA, IZHAR AHMAD RATHER, AMATUL MUHEE, TAWHEED SHAFI, MOHD IQBAL YATOO
Contagious caprine pleuropneumonia causes oxidative stress and immune disturbances in affected goats. Hence these derogative alterations aggravate the pathogenesis and severity of this disease, thus, necessitating utilization of antioxidants and/or immunomodulators in the therapeutic regimes of contagious caprine pleuropneumonia. Present study evaluated ameliorative effects of N-acetyl cysteine (NAC) and alpha tocopherol (vitamin E) in association with primary antibiotic tylosin treatment in contagious caprine pleuropneumonia affected test goats in comparison to antibiotic only treated goats (n=6) and healthy goats (n=6). N-acetyl cysteine given at a dose of 12 mg per kg of body weight orally daily for 7 days, showed better antioxidant effect by significantly ameliorating oxidative stress (41.4%) compared to other treatments; besides, decreasing total oxidative status (TOS) and increasing total antioxidant status (TAS). Immunomodulatory potential of alpha tocopherol given at a dose of 10 mg per kg of body weight orally daily for 7 days, was found to be comparatively better than NAC in decreasing percentage of neutrophils and increasing percentage of lymphocytes; besides, decreasing the levels of tumor necrosis factor-α (TNF-α) and immunoglobulin G (IgG) when the modulation of total leukocyte count and differential leukocyte count was comparable between the two. Thus, it can be concluded from this study that NAC acts as a comparatively better antioxidant and alpha tocopherol as better immunomodulator when used in the therapeutic regimes of contagious caprine pleuropneumonia.
{"title":"Evaluation of ameliorative potential of antioxidants and/or immunomodulators in contagious caprine pleuropneumonia affected Himalayan Pashmina goats","authors":"OVEAS RAFFIQ PARRAY, None MUHEET, RIYAZ AHMAD BHAT, HAMID ULLAH MALIK, SABIA QURESHI, SHAFAYAT AHMAD BEIG, QURAT UL AIN NAZIR, MAJID SHAFI KAWOOSA, IZHAR AHMAD RATHER, AMATUL MUHEE, TAWHEED SHAFI, MOHD IQBAL YATOO","doi":"10.56093/ijans.v93i9.110402","DOIUrl":"https://doi.org/10.56093/ijans.v93i9.110402","url":null,"abstract":"Contagious caprine pleuropneumonia causes oxidative stress and immune disturbances in affected goats. Hence these derogative alterations aggravate the pathogenesis and severity of this disease, thus, necessitating utilization of antioxidants and/or immunomodulators in the therapeutic regimes of contagious caprine pleuropneumonia. Present study evaluated ameliorative effects of N-acetyl cysteine (NAC) and alpha tocopherol (vitamin E) in association with primary antibiotic tylosin treatment in contagious caprine pleuropneumonia affected test goats in comparison to antibiotic only treated goats (n=6) and healthy goats (n=6). N-acetyl cysteine given at a dose of 12 mg per kg of body weight orally daily for 7 days, showed better antioxidant effect by significantly ameliorating oxidative stress (41.4%) compared to other treatments; besides, decreasing total oxidative status (TOS) and increasing total antioxidant status (TAS). Immunomodulatory potential of alpha tocopherol given at a dose of 10 mg per kg of body weight orally daily for 7 days, was found to be comparatively better than NAC in decreasing percentage of neutrophils and increasing percentage of lymphocytes; besides, decreasing the levels of tumor necrosis factor-α (TNF-α) and immunoglobulin G (IgG) when the modulation of total leukocyte count and differential leukocyte count was comparable between the two. Thus, it can be concluded from this study that NAC acts as a comparatively better antioxidant and alpha tocopherol as better immunomodulator when used in the therapeutic regimes of contagious caprine pleuropneumonia.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135740308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The objective of the study is to evaluate the comparative detection efficacy of primers targeting SpeI-AvaI restriction fragment and small subunit ribosomal RNA (SSU rRNA) gene of Babesia bigemina by employing conventional polymerase chain reaction (PCR) on 783 animals (296 cattle and 487 buffaloes) of low lying (bet) area of Punjab. The detection rate of SpeI-AvaI and SSU rRNA PCR assays was 3.96% (31/783), and 6.64% (52/783), respectively. Among cattle and buffaloes, prevalence of B. bigemina was higher (P<0.01) in cattle by both the primers. The sensitivity and specificity of SSU rRNA PCR as compared to SpeI-AvaI restriction fragment PCR was 100% and 97.2%, respectively. The blast analysis of the nucleotides of the sequenced amplicons of Ludhiana isolates of SpeI-AvaI and SSU rRNA PCR assay of B. bigemina showed 83 and 100% similarity with available sequence in Genbank. The analysis of evolutionary divergence revealed that range of divergence was lying between 0.000 to 0.011 between SSU rRNA sequence with the other sequences of B. bigemina as well as Babesia species. To conclude, the primers targeting SSU rRNA gene are a better tool for amplification of the B. bigemina.
{"title":"Comparative detection efficacy of primers targeting SpeI-AvaI restriction fragment and small subunit ribosomal RNA gene of Babesia bigemina","authors":"PARAMJIT KAUR, PRAYAG DUTT JUYAL, AMRITA SHARMA, LACHHMAN DAS SINGLA, CHANDRA SEKHAR MUKHOPADHYAY","doi":"10.56093/ijans.v92i7.109919","DOIUrl":"https://doi.org/10.56093/ijans.v92i7.109919","url":null,"abstract":"The objective of the study is to evaluate the comparative detection efficacy of primers targeting SpeI-AvaI restriction fragment and small subunit ribosomal RNA (SSU rRNA) gene of Babesia bigemina by employing conventional polymerase chain reaction (PCR) on 783 animals (296 cattle and 487 buffaloes) of low lying (bet) area of Punjab. The detection rate of SpeI-AvaI and SSU rRNA PCR assays was 3.96% (31/783), and 6.64% (52/783), respectively. Among cattle and buffaloes, prevalence of B. bigemina was higher (P<0.01) in cattle by both the primers. The sensitivity and specificity of SSU rRNA PCR as compared to SpeI-AvaI restriction fragment PCR was 100% and 97.2%, respectively. The blast analysis of the nucleotides of the sequenced amplicons of Ludhiana isolates of SpeI-AvaI and SSU rRNA PCR assay of B. bigemina showed 83 and 100% similarity with available sequence in Genbank. The analysis of evolutionary divergence revealed that range of divergence was lying between 0.000 to 0.011 between SSU rRNA sequence with the other sequences of B. bigemina as well as Babesia species. To conclude, the primers targeting SSU rRNA gene are a better tool for amplification of the B. bigemina.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135016418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-02DOI: 10.56093/ijans.v92i7.132873
AHMAD FURQON, SUYADI SUYADI, KUSWATI KUSWATI, SUTIKNO SUTIKNO, FERDY SAPUTRA, WIKE ANDRE SEPTIAN
Heat shock proteins (HSPs) associated with stress reactions play an important role in cell survival by activating numerous regulatory proteins and inducing black apoptosis. This study aimed to identify the potential SNPs of HSP70 gene in Kacang goats in Indonesia. Forty-three Kacang goats were selected from Sidoarjo and Tulungagung districts. The DNA isolated from blood samples was successfully amplified using the polymerase chain reaction (PCR) method with a pair of primers. The PCR products were sequenced in the coding region. The sequences were successfully aligned to determine the potential SNPs. A novel SNP (c.1311C>T) was found in this study. This SNP was categorized as a synonymous mutation. The insertion-deletion (in-del) mutation was also observed at the 1151stand 1161st nucleotide positions. Based on these mutations, four haplotypes were constructed where haplotype 1 had the highest frequency in Sidoarjo. Indonesian goats had no close relation with Iraqi goats according to Neighbor-Joing with Kimura’s 2-parameter approach. Haplotypes 1 and 2 in Indonesian goats had three different bases with all haplotypes in Iraqi goats in Median Joining Network. The study concluded that a novel SNP of HSP70 gene was identified in Kacang goats.
{"title":"A novel SNP (c.1311C>T) on heat shock protein 70 (HSP70) gene of Kacang goat in Indonesia","authors":"AHMAD FURQON, SUYADI SUYADI, KUSWATI KUSWATI, SUTIKNO SUTIKNO, FERDY SAPUTRA, WIKE ANDRE SEPTIAN","doi":"10.56093/ijans.v92i7.132873","DOIUrl":"https://doi.org/10.56093/ijans.v92i7.132873","url":null,"abstract":"Heat shock proteins (HSPs) associated with stress reactions play an important role in cell survival by activating numerous regulatory proteins and inducing black apoptosis. This study aimed to identify the potential SNPs of HSP70 gene in Kacang goats in Indonesia. Forty-three Kacang goats were selected from Sidoarjo and Tulungagung districts. The DNA isolated from blood samples was successfully amplified using the polymerase chain reaction (PCR) method with a pair of primers. The PCR products were sequenced in the coding region. The sequences were successfully aligned to determine the potential SNPs. A novel SNP (c.1311C>T) was found in this study. This SNP was categorized as a synonymous mutation. The insertion-deletion (in-del) mutation was also observed at the 1151stand 1161st nucleotide positions. Based on these mutations, four haplotypes were constructed where haplotype 1 had the highest frequency in Sidoarjo. Indonesian goats had no close relation with Iraqi goats according to Neighbor-Joing with Kimura’s 2-parameter approach. Haplotypes 1 and 2 in Indonesian goats had three different bases with all haplotypes in Iraqi goats in Median Joining Network. The study concluded that a novel SNP of HSP70 gene was identified in Kacang goats.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135064440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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