Metal hyperaccumulators (HAs) are renowned for their remarkable capacity to sequester heavy metals (HMs) within their tissues, a trait that is closely linked to their symbiotic relationship with a unique set of microbes inhabiting their rhizosphere and endosphere. Despite the pivotal role of HAs in the remediation of HMs, the intricacies of the microbiome characteristics associated with HAs remain largely elusive. This review navigates the intricate microbial landscape of the rhizosphere associated with hyperaccumulating plants. Emphasizing the assembly and colonization dynamics of microbiomes by HAs, it sheds light on the substantial variations in community structure and function during exposure to HMs. Furthermore, this study delves into the multifaceted role of HA-associated microbes in HMs remediation, elucidating both direct and indirect mechanisms employed by these microbial communities in assisting phytoremediation. By providing new insights into the interaction between HAs and their microbial partners, this review highlights the vital contributions of these symbiotic relationships to the efficient extraction and detoxification of toxic metals through phytoremediation.
金属超积累植物（HAs）因其在组织内封存重金属（HMs）的非凡能力而闻名于世，这种特性与它们与栖息在根圈和内圈的一系列独特微生物的共生关系密切相关。尽管有害藻类在修复有害藻类方面发挥着关键作用，但与有害藻类相关的微生物群特征的复杂性在很大程度上仍然难以捉摸。本综述介绍了与高积累植物相关的根瘤菌层错综复杂的微生物景观。它强调了 HAs 微生物组的组装和定植动态，揭示了暴露于 HMs 期间群落结构和功能的巨大变化。此外，这项研究还深入探讨了 HA 相关微生物在 HMs 修复中的多方面作用，阐明了这些微生物群落在协助植物修复中采用的直接和间接机制。本综述提供了有关 HMs 与其微生物伙伴之间相互作用的新见解，强调了这些共生关系对通过植物修复有效提取和解毒有毒金属的重要贡献。
{"title":"Exploring plant symbiotic microbial dynamics in metal hyperaccumulators for phytoremediation","authors":"Sarita Tiwari , Abid Ullah , Yu-Xi Feng, Xiao-Zhang Yu","doi":"10.1016/j.ibiod.2024.105941","DOIUrl":"10.1016/j.ibiod.2024.105941","url":null,"abstract":"<div><div>Metal hyperaccumulators (HAs) are renowned for their remarkable capacity to sequester heavy metals (HMs) within their tissues, a trait that is closely linked to their symbiotic relationship with a unique set of microbes inhabiting their rhizosphere and endosphere. Despite the pivotal role of HAs in the remediation of HMs, the intricacies of the microbiome characteristics associated with HAs remain largely elusive. This review navigates the intricate microbial landscape of the rhizosphere associated with hyperaccumulating plants. Emphasizing the assembly and colonization dynamics of microbiomes by HAs, it sheds light on the substantial variations in community structure and function during exposure to HMs. Furthermore, this study delves into the multifaceted role of HA-associated microbes in HMs remediation, elucidating both direct and indirect mechanisms employed by these microbial communities in assisting phytoremediation. By providing new insights into the interaction between HAs and their microbial partners, this review highlights the vital contributions of these symbiotic relationships to the efficient extraction and detoxification of toxic metals through phytoremediation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105941"},"PeriodicalIF":4.1,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-14DOI: 10.1016/j.ibiod.2024.105939
Tadele Assefa Aragaw , Carolina Suarez , Addis Simachew , Catherine J. Paul
Sequential anaerobic/aerobic (A/O) treatment conditions for textile wastewater (WW) are more effective than conventional biological treatment. Anaerobic treatment is essential because anaerobic microbes can first break down complex and recalcitrant compounds, which are difficult to degrade under aerobic conditions. The simpler, more degradable compounds are then further broken down by aerobic microbes. This study aimed to evaluate the performance of a sequential A/O treatment process using a pilot-scale reactor to treat real textile WW and to characterize reactor and inoculum microbial community structures. The reactors were inoculated with microbial consortia originating from a diverse alkaliphilic soda lake in the Ethiopian Rift Valley. The WW test parameters were used to evaluate the performance of the treatment process. At steady state, the removal efficiencies were 97 % for dye, 86 % for Chemical Oxygen Demand (COD), and 93 % for Total Kjeldahl Nitrogen (TKN). Amplicon sequencing revealed that Firmicutes, Proteobacteria, and Actinobacteria were the dominant phyla in all the samples. Uncategorized microorganisms, followed by Alkalibacterium, Bifidobacterium, and Clostridium were the most abundant taxon in all the samples. The microbial community detected during the treatment process was not abundant in the inoculum originating from Lake Chitu, suggesting that the communities likely originated from textile WW. The textile WW treated with the integrated A/O process effectively degraded dyes, and the inoculated microbes demonstrated resistance to the toxic chemical composition of the WW. The integrated treatment process, along with the alkaliphilic microbial consortia, has proven to be practical for treating textile WW, offering valuable insights for field-scale applications.
{"title":"The potential of alkaline tolerant microbial consortia for textile wastewater treatment under integrated anaerobic/aerobic conditions: Performance evaluation and microbial community analysis","authors":"Tadele Assefa Aragaw , Carolina Suarez , Addis Simachew , Catherine J. Paul","doi":"10.1016/j.ibiod.2024.105939","DOIUrl":"10.1016/j.ibiod.2024.105939","url":null,"abstract":"<div><div>Sequential anaerobic/aerobic (A/O) treatment conditions for textile wastewater (WW) are more effective than conventional biological treatment. Anaerobic treatment is essential because anaerobic microbes can first break down complex and recalcitrant compounds, which are difficult to degrade under aerobic conditions. The simpler, more degradable compounds are then further broken down by aerobic microbes. This study aimed to evaluate the performance of a sequential A/O treatment process using a pilot-scale reactor to treat real textile WW and to characterize reactor and inoculum microbial community structures. The reactors were inoculated with microbial consortia originating from a diverse alkaliphilic soda lake in the Ethiopian Rift Valley. The WW test parameters were used to evaluate the performance of the treatment process. At steady state, the removal efficiencies were 97 % for dye, 86 % for Chemical Oxygen Demand (COD), and 93 % for Total Kjeldahl Nitrogen (TKN). Amplicon sequencing revealed that <em>Firmicutes</em>, <em>Proteobacteria,</em> and <em>Actinobacteria were</em> the dominant phyla in all the samples. Uncategorized microorganisms, followed by <em>Alkalibacterium, Bifidobacterium,</em> and <em>Clostridium</em> were the most abundant taxon in all the samples. The microbial community detected during the treatment process was not abundant in the inoculum originating from Lake Chitu, suggesting that the communities likely originated from textile WW. The textile WW treated with the integrated A/O process effectively degraded dyes, and the inoculated microbes demonstrated resistance to the toxic chemical composition of the WW. The integrated treatment process, along with the alkaliphilic microbial consortia, has proven to be practical for treating textile WW, offering valuable insights for field-scale applications.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105939"},"PeriodicalIF":4.1,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-14DOI: 10.1016/j.ibiod.2024.105940
Shu-Hui Liu , Jin-Shuo Liu , Chi-Wen Lin
Heavy metals accumulate in sediments, and there is a need to develop environmentally friendly methods for treating these pollutants. Sediment microbial fuel cells (SMFCs) generate electrons and electric fields through the degradation of organic pollutants by anodic microorganisms. The electric field causes positively charged heavy metals to migrate to the negatively charged cathode surface and receive electrons to reduce to a less toxic form, thus enabling sediment remediation without external power input. This study introduces the research progress of SMFCs for heavy metal remediation from the following aspects: (i) by summarizing the reaction principles of SMFCs; (ii) clarifying the factors affecting heavy metal remediation; (iii) comparing the performances of two SMFC configurations: vertical configuration: the anode and cathode are, respectively, placed in the sediment and overlying water; horizontal configuration: they are both placed in the sediment layer; (iv) illustrating the state-of-the-art research progress of SMFCs for heavy metal remediation. In contrast to other heavy metal removal technologies by electrochemical treatments, SMFCs provide a bioelectrochemical means to achieve zero-carbon emission treatment by integrating with other promising technologies. Therefore, this study addresses SMFC challenges and bridges research and development gaps.
{"title":"Heavy metal removal and recovery from contaminated sediments based on bioelectrochemical systems: Insights, progress, and perspectives","authors":"Shu-Hui Liu , Jin-Shuo Liu , Chi-Wen Lin","doi":"10.1016/j.ibiod.2024.105940","DOIUrl":"10.1016/j.ibiod.2024.105940","url":null,"abstract":"<div><div>Heavy metals accumulate in sediments, and there is a need to develop environmentally friendly methods for treating these pollutants. Sediment microbial fuel cells (SMFCs) generate electrons and electric fields through the degradation of organic pollutants by anodic microorganisms. The electric field causes positively charged heavy metals to migrate to the negatively charged cathode surface and receive electrons to reduce to a less toxic form, thus enabling sediment remediation without external power input. This study introduces the research progress of SMFCs for heavy metal remediation from the following aspects: (i) by summarizing the reaction principles of SMFCs; (ii) clarifying the factors affecting heavy metal remediation; (iii) comparing the performances of two SMFC configurations: vertical configuration: the anode and cathode are, respectively, placed in the sediment and overlying water; horizontal configuration: they are both placed in the sediment layer; (iv) illustrating the state-of-the-art research progress of SMFCs for heavy metal remediation. In contrast to other heavy metal removal technologies by electrochemical treatments, SMFCs provide a bioelectrochemical means to achieve zero-carbon emission treatment by integrating with other promising technologies. Therefore, this study addresses SMFC challenges and bridges research and development gaps.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105940"},"PeriodicalIF":4.1,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-11DOI: 10.1016/j.ibiod.2024.105934
Zi-Yue Fu , Wen-Tao Chen , Gui-Na Qi , Zhao-wei Hou , Yi-Fan Liu , Li-Bin Shou , Lei Zhou , Shi-Zhong Yang , Xiao-Lin Wu , Ji-Dong Gu , Bo-Zhong Mu
Current studies on Microbial Enhanced Oil Recovery (MEOR) mainly focus on introducing exogenous microbes or injecting biosurfactants into oil reservoirs, whereas simulating indigenous oil-displacing microorganisms has been less explored. In this study, we conducted MEOR applications on an oil reservoir in Daqing oilfield by injection of only nutrition solution (without microbial strains or biosurfactants), leading to an average increase of 218.6–221.4% in oil production. An alternative stable microbial community dominated by Pseudomonas species was established after MEOR applications, even one year after the termination of the injection. Additionally, members of Ca. Patescibacteria, Ca. Caldatribacteriota (JS1) and hydrogenotrophic methanogens Methanocorpusculum and Methanolinea were found as biomarkers for high oil productivity. These results demonstrate the potential to enhance crude oil production through biosurfactant production, acid and alcohol metabolites production, hydrocarbon degradation and biogas (CH4 and N2) production by the aforementioned stimulated indigenous oil-displacing microorganisms. This research provides theoretical guidance for the exploitation of depleted reservoirs and the extension of the development life of oil reservoirs.
{"title":"Microbiome changes and characteristics under nutrient injection for enhanced oil production at Daqing oilfield","authors":"Zi-Yue Fu , Wen-Tao Chen , Gui-Na Qi , Zhao-wei Hou , Yi-Fan Liu , Li-Bin Shou , Lei Zhou , Shi-Zhong Yang , Xiao-Lin Wu , Ji-Dong Gu , Bo-Zhong Mu","doi":"10.1016/j.ibiod.2024.105934","DOIUrl":"10.1016/j.ibiod.2024.105934","url":null,"abstract":"<div><div>Current studies on Microbial Enhanced Oil Recovery (MEOR) mainly focus on introducing exogenous microbes or injecting biosurfactants into oil reservoirs, whereas simulating indigenous oil-displacing microorganisms has been less explored. In this study, we conducted MEOR applications on an oil reservoir in Daqing oilfield by injection of only nutrition solution (without microbial strains or biosurfactants), leading to an average increase of 218.6–221.4% in oil production. An alternative stable microbial community dominated by <em>Pseudomonas</em> species was established after MEOR applications, even one year after the termination of the injection. Additionally, members of <em>Ca.</em> Patescibacteria, <em>Ca</em>. Caldatribacteriota (JS1) and hydrogenotrophic methanogens <em>Methanocorpusculum</em> and <em>Methanolinea</em> were found as biomarkers for high oil productivity. These results demonstrate the potential to enhance crude oil production through biosurfactant production, acid and alcohol metabolites production, hydrocarbon degradation and biogas (CH<sub>4</sub> and N<sub>2</sub>) production by the aforementioned stimulated indigenous oil-displacing microorganisms. This research provides theoretical guidance for the exploitation of depleted reservoirs and the extension of the development life of oil reservoirs.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105934"},"PeriodicalIF":4.1,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-07DOI: 10.1016/j.ibiod.2024.105938
S.V. Nagarathna , T.M. Chandramouli Swamy , Pooja V. Reddy , Santosh R. Kanade , Anand S. Nayak
Benzo[a]pyrene (BaP) is a persistent carcinogenic environmental pollutant with high bioaccumulation potential and is resistant to bacterial biodegradation. Therefore, its removal from the biosphere is a priority. In the current study, the bacterial culture Paenibacillus sp. PRNK-6 was evaluated for the degradation of BaP. Paenibacillus sp. PRNK-6 efficiently utilizes BaP as a sole carbon source and degrades 89.43% of BaP within 120 h at an initial concentration of 100 mg L−1. Maximum growth was observed at 96 h with 28.96 × 107 colony-forming units (CFU). The BaP metabolic intermediates were characterized by High-performance liquid chromatography (HPLC) and, Gas chromatography-mass spectrometry (GC-MS). Based on the metabolite characterization, utilization of probable metabolic intermediates, and investigation of the enzyme involved, a putative pathway of the BaP degradation in PRNK-6 was proposed. The metabolites formed includes a novel ring cleavage metabolite phenalene-1,9-dicarboxylic acid. The two terminal monoaromatic metabolites catechol and protocatechuate (PCA) undergo ring fission by catechol 1,2-dioxygenase and protocatechuate 3,4-dioxygenase, individually and get into the tricarboxylic acid (TCA) cycle. In both pathways there is no accumulation of any dead-end products. The results suggest that the strain PRNK-6 could be a promising biodegradation tool for high molecular weight polycyclic aromatic hydrocarbons (HMW PAHs) like BaP and may be equally used for bioremediation of other polycyclic aromatic hydrocarbons (PAHs).
{"title":"Metabolism of Benzo[a]pyrene by Paenibacillus sp. PRNK-6 through novel metabolite phenalene-1,9-dicarboxylic acid","authors":"S.V. Nagarathna , T.M. Chandramouli Swamy , Pooja V. Reddy , Santosh R. Kanade , Anand S. Nayak","doi":"10.1016/j.ibiod.2024.105938","DOIUrl":"10.1016/j.ibiod.2024.105938","url":null,"abstract":"<div><div>Benzo[a]pyrene (BaP) is a persistent carcinogenic environmental pollutant with high bioaccumulation potential and is resistant to bacterial biodegradation. Therefore, its removal from the biosphere is a priority. In the current study, the bacterial culture <em>Paenibacillus</em> sp. PRNK-6 was evaluated for the degradation of BaP. <em>Paenibacillus</em> sp. PRNK-6 efficiently utilizes BaP as a sole carbon source and degrades 89.43% of BaP within 120 h at an initial concentration of 100 mg L<sup>−1</sup>. Maximum growth was observed at 96 h with 28.96 × 10<sup>7</sup> colony-forming units (CFU). The BaP metabolic intermediates were characterized by High-performance liquid chromatography (HPLC) and, Gas chromatography-mass spectrometry (GC-MS). Based on the metabolite characterization, utilization of probable metabolic intermediates, and investigation of the enzyme involved, a putative pathway of the BaP degradation in PRNK-6 was proposed. The metabolites formed includes a novel ring cleavage metabolite phenalene-1,9-dicarboxylic acid. The two terminal monoaromatic metabolites catechol and protocatechuate (PCA) undergo ring fission by catechol 1,2-dioxygenase and protocatechuate 3,4-dioxygenase, individually and get into the tricarboxylic acid (TCA) cycle. In both pathways there is no accumulation of any dead-end products. The results suggest that the strain PRNK-6 could be a promising biodegradation tool for high molecular weight polycyclic aromatic hydrocarbons (HMW PAHs) like BaP and may be equally used for bioremediation of other polycyclic aromatic hydrocarbons (PAHs).</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105938"},"PeriodicalIF":4.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-07DOI: 10.1016/j.ibiod.2024.105932
Xiaopeng Qiu , Zijun Liu , Xianpeng Li , Yangtao Wang , Xingtong Wang , Yaozhong Zhang , Jiake Li , Xiaoliang Li , Xin Cao , Xing Zheng
This study explores the specific impact of bound and dissolved extracellular organic matter (bEOM and dEOM), and their collective influence with algal cells on membrane fouling during ultrafiltration (UF) and coagulation-UF of algae-laden water. By characterizing the organic properties and adsorptive behaviors of bEOM and dEOM, and analyzing their subsequent impacts, we clarify their roles and contributions to membrane fouling. Our analyses revealed that bEOM, characterized by its higher molecular weight (MW) and hydrophobic nature, contains more protein-like substances compared to dEOM. Quartz Crystal Microbalance with Dissipation (QCM-D) analysis highlights significant differences in their adsorption behaviors, with bEOM demonstrating greater adhesion and higher adsorptive fouling potential. Despite bEOM's lower concentration relative to dEOM, at a ratio of 0.12–1, their contributions to irreversible membrane resistance are nearly identical, at 30.7% and 30.9% respectively, in the UF of algae-laden water. Coagulation pretreatment effectively reduces bEOM's fouling potential by forming larger flocs, thus minimizing its contact with the membrane. In terms of irreversible membrane resistance, the contributions are 6.5% from cells, 24.8% from bEOM, and 68.6% from dEOM. The presence of dEOM complicates coagulation efficiency due to its low MW components and high hydrophilicity. Using the Hermia model, atomic force microscopy (AFM), and scanning electron microscopy (SEM), we demonstrated how bEOM and dEOM modify membrane fouling mechanisms, particularly by influencing cake layer formation. These insights emphasize the distinct and significant contributions of bEOM and dEOM to membrane fouling, necessitating targeted strategies for their management to enhance the efficiency and sustainability of UF systems in water treatment facilities.
{"title":"Contribution of specific extracellular organic matter on membrane fouling in ultrafiltration and coagulation-ultrafiltration of algae-laden water","authors":"Xiaopeng Qiu , Zijun Liu , Xianpeng Li , Yangtao Wang , Xingtong Wang , Yaozhong Zhang , Jiake Li , Xiaoliang Li , Xin Cao , Xing Zheng","doi":"10.1016/j.ibiod.2024.105932","DOIUrl":"10.1016/j.ibiod.2024.105932","url":null,"abstract":"<div><div>This study explores the specific impact of bound and dissolved extracellular organic matter (bEOM and dEOM), and their collective influence with algal cells on membrane fouling during ultrafiltration (UF) and coagulation-UF of algae-laden water. By characterizing the organic properties and adsorptive behaviors of bEOM and dEOM, and analyzing their subsequent impacts, we clarify their roles and contributions to membrane fouling. Our analyses revealed that bEOM, characterized by its higher molecular weight (MW) and hydrophobic nature, contains more protein-like substances compared to dEOM. Quartz Crystal Microbalance with Dissipation (QCM-D) analysis highlights significant differences in their adsorption behaviors, with bEOM demonstrating greater adhesion and higher adsorptive fouling potential. Despite bEOM's lower concentration relative to dEOM, at a ratio of 0.12–1, their contributions to irreversible membrane resistance are nearly identical, at 30.7% and 30.9% respectively, in the UF of algae-laden water. Coagulation pretreatment effectively reduces bEOM's fouling potential by forming larger flocs, thus minimizing its contact with the membrane. In terms of irreversible membrane resistance, the contributions are 6.5% from cells, 24.8% from bEOM, and 68.6% from dEOM. The presence of dEOM complicates coagulation efficiency due to its low MW components and high hydrophilicity. Using the Hermia model, atomic force microscopy (AFM), and scanning electron microscopy (SEM), we demonstrated how bEOM and dEOM modify membrane fouling mechanisms, particularly by influencing cake layer formation. These insights emphasize the distinct and significant contributions of bEOM and dEOM to membrane fouling, necessitating targeted strategies for their management to enhance the efficiency and sustainability of UF systems in water treatment facilities.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105932"},"PeriodicalIF":4.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-07DOI: 10.1016/j.ibiod.2024.105936
Vijendra Shah , Achlesh Daverey
In present study, response surface methodology (RSM) based central composite design (CCD) was used to study the interactive effect between two individual soil amendments, sophorolipids (SLs) and pine needle biochar for the phytoremediation of Cd contamination and enzymatic activity of soil. The experimented concentrations of SLs and biochar ranged from 0.5 to 2.6 g/kg and 0.4–1.8 %, respectively. The SLs and biochar at the concentration 1.1 g/kg and 1.55 % respectively exhibit the maximum uptake in shoot (125.33 mg/kg) and root (298.27 mg/kg). The results also showed higher R2 (>0.9) for Cd uptake in B. pilosa and R2 (>0.85) for soil enzymatic activity using model generated by CCD of RSM. These results signify the reliability of the model and suggested that this model could be used for the prediction of increased metal uptake by plants. The optimum concentrations of SLs and biochar predicted by the CCD were 1.23 g/kg and 1.55 %, respectively with desirability score of 1 for the uptake of Cd in B. pilosa. The results highlighted that application of these amendments can be a pivotal step in the direction of remediation of heavy metal contamination from soil at larger scale.
{"title":"Assessment of interactive effects of sophorolipids and pine needle biochar augmentation in the process of phytoremediation of Cd contaminated soil using response surface methodology","authors":"Vijendra Shah , Achlesh Daverey","doi":"10.1016/j.ibiod.2024.105936","DOIUrl":"10.1016/j.ibiod.2024.105936","url":null,"abstract":"<div><div>In present study, response surface methodology (RSM) based central composite design (CCD) was used to study the interactive effect between two individual soil amendments, sophorolipids (SLs) and pine needle biochar for the phytoremediation of Cd contamination and enzymatic activity of soil. The experimented concentrations of SLs and biochar ranged from 0.5 to 2.6 g/kg and 0.4–1.8 %, respectively. The SLs and biochar at the concentration 1.1 g/kg and 1.55 % respectively exhibit the maximum uptake in shoot (125.33 mg/kg) and root (298.27 mg/kg). The results also showed higher <em>R</em><sup><em>2</em></sup> (>0.9) for Cd uptake in <em>B. pilosa</em> and <em>R</em><sup><em>2</em></sup> (>0.85) for soil enzymatic activity using model generated by CCD of RSM. These results signify the reliability of the model and suggested that this model could be used for the prediction of increased metal uptake by plants. The optimum concentrations of SLs and biochar predicted by the CCD were 1.23 g/kg and 1.55 %, respectively with desirability score of 1 for the uptake of Cd in <em>B. pilosa</em>. The results highlighted that application of these amendments can be a pivotal step in the direction of remediation of heavy metal contamination from soil at larger scale.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105936"},"PeriodicalIF":4.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-07DOI: 10.1016/j.ibiod.2024.105933
Xueting Wang , Shihao Liu , Xiaoke Ding , Lele Zhang , Xueru Lv , Jie Li , Caihong Song , Chen Zhang , Shenghui Wang
The aromatic compound p-cresol is a notorious industrial pollutant characterized by its high toxicity, persistence and bioaccumulation within higher organisms. A thorough understanding of the microbial metabolic pathways involved in p-cresol degradation is critical for the design and optimization of microbial wastewater treatment systems. Despite numerous studies on the degradation pathways of p-cresol by various microbial species, the metabolic diversity within a single strain remains largely unexplored. This study investigated the metabolic mechanism of p-cresol in Bacillus subtilis ZW, a bacterium capable of degrading p-cresol. Through LC-MS analysis, we identified twelve distinct metabolic intermediates in the culture of strain ZW, leading to the proposal of three plausible degradation pathways. These include methyl hydroxylation, direct aromatic ring hydroxylation, and phosphorylation of the hydroxyl group, and all of which may concurrently contribute to p-cresol biodegradation by strain ZW. Further study showed that the genome of strain ZW harbored 47 coding genes associated with the degradation of p-cresol and its structural analogs, underscoring the metabolic versatility of this strain and its potential for xenobiotic biodegradation. These findings contribute valuable insights into the biodegradation mechanisms of pollutants. Under optimal degradation conditions of 35 °C and pH 7.0, strain ZW demonstrated the capacity to metabolize 27.5 % of p-cresol (10 mg/L) in minimal salt media within a week, and was capable of completely degrading 10 mg/L p-cresol in wastewater within five days. The potential utility of strain ZW in the bioremediation of p-cresol and other aromatic compounds is thus evident.
{"title":"Coexistence of diverse metabolic pathways promotes p-cresol biodegradation by Bacillus subtilis ZW","authors":"Xueting Wang , Shihao Liu , Xiaoke Ding , Lele Zhang , Xueru Lv , Jie Li , Caihong Song , Chen Zhang , Shenghui Wang","doi":"10.1016/j.ibiod.2024.105933","DOIUrl":"10.1016/j.ibiod.2024.105933","url":null,"abstract":"<div><div>The aromatic compound p-cresol is a notorious industrial pollutant characterized by its high toxicity, persistence and bioaccumulation within higher organisms. A thorough understanding of the microbial metabolic pathways involved in p-cresol degradation is critical for the design and optimization of microbial wastewater treatment systems. Despite numerous studies on the degradation pathways of p-cresol by various microbial species, the metabolic diversity within a single strain remains largely unexplored. This study investigated the metabolic mechanism of p-cresol in <em>Bacillus subtilis</em> ZW, a bacterium capable of degrading p-cresol. Through LC-MS analysis, we identified twelve distinct metabolic intermediates in the culture of strain ZW, leading to the proposal of three plausible degradation pathways. These include methyl hydroxylation, direct aromatic ring hydroxylation, and phosphorylation of the hydroxyl group, and all of which may concurrently contribute to p-cresol biodegradation by strain ZW. Further study showed that the genome of strain ZW harbored 47 coding genes associated with the degradation of p-cresol and its structural analogs, underscoring the metabolic versatility of this strain and its potential for xenobiotic biodegradation. These findings contribute valuable insights into the biodegradation mechanisms of pollutants. Under optimal degradation conditions of 35 °C and pH 7.0, strain ZW demonstrated the capacity to metabolize 27.5 % of p-cresol (10 mg/L) in minimal salt media within a week, and was capable of completely degrading 10 mg/L p-cresol in wastewater within five days. The potential utility of strain ZW in the bioremediation of p-cresol and other aromatic compounds is thus evident.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105933"},"PeriodicalIF":4.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-05DOI: 10.1016/j.ibiod.2024.105931
Jianzhao Li , Yangfan Xu , Longfei Li , Naidong Xiao , Mengjie Qu , Xiaoqiong Wan , Yumei Hua , Jianwei Zhao
Complete ammonia oxidizers (comammox bacteria) can convert ammonia into nitric acid through single-step nitrification. This study explored the spatial variations of comammox bacteria in the lakeshore area of the Houguan Lake in Wuhan, China. The abundance of the two comammox bacteria clades and two traditional ammonia-oxidizing microorganisms, ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), generally showed a gradually decreasing trend from the shore to the water. Moreover, a similar decreasing trend was observed for the respective and total nitrification rate of three types of ammonia-oxidizing microorganisms. The average nitrification rate of AOA, AOB and comammox bacteria was 0.568, 0.718, and 0.935 mg N kg−1 d−1, respectively. Besides, comammox bacteria exhibited a high biological diversity, with clade A and clade B and three subclades of clade A all present. Among different clades, clade B played a dominant role in the ammonia oxidation process. Both the abundance and nitrification rate of comammox bacteria were significantly positively correlated with total carbon and total nitrogen, indicating that these two nutrient substances are important factors influencing this microorganism. Our results demonstrate that the spatial variations of environmental elements in the lakeshore area lead to gradual decreases of comammox bacteria from the shore to the water.
完全氨氧化细菌（comammox bacteria）可通过单步硝化作用将氨转化为硝酸。本研究探讨了中国武汉后官湖湖岸地区氨氧化细菌的空间变化。研究结果表明，两种复合氧化细菌支系以及两种传统的氨氧化微生物--氨氧化古细菌（AOA）和氨氧化细菌（AOB）的丰度总体上呈现出从湖岸向水体逐渐降低的趋势。此外，三种氨氧化微生物的各自硝化率和总硝化率也呈类似的下降趋势。AOA 菌、AOB 菌和兼氧菌的平均硝化速率分别为 0.568、0.718 和 0.935 毫克 N kg-1 d-1。此外，复合氧化细菌表现出较高的生物多样性，A 支系、B 支系和 A 支系的三个亚支系均存在。在不同支系中，支系 B 在氨氧化过程中起主导作用。复合氧化细菌的丰度和硝化率均与总碳和总氮呈显著正相关，表明这两种营养物质是影响该微生物的重要因素。我们的研究结果表明，湖岸地区环境要素的空间变化导致彗星菌从湖岸向水中逐渐减少。
{"title":"Shore-to-water spatial variations of complete ammonia oxidizers in a lake in Wuhan, China","authors":"Jianzhao Li , Yangfan Xu , Longfei Li , Naidong Xiao , Mengjie Qu , Xiaoqiong Wan , Yumei Hua , Jianwei Zhao","doi":"10.1016/j.ibiod.2024.105931","DOIUrl":"10.1016/j.ibiod.2024.105931","url":null,"abstract":"<div><div>Complete ammonia oxidizers (comammox bacteria) can convert ammonia into nitric acid through single-step nitrification. This study explored the spatial variations of comammox bacteria in the lakeshore area of the Houguan Lake in Wuhan, China. The abundance of the two comammox bacteria clades and two traditional ammonia-oxidizing microorganisms, ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), generally showed a gradually decreasing trend from the shore to the water. Moreover, a similar decreasing trend was observed for the respective and total nitrification rate of three types of ammonia-oxidizing microorganisms. The average nitrification rate of AOA, AOB and comammox bacteria was 0.568, 0.718, and 0.935 mg N kg<sup>−1</sup> d<sup>−1</sup>, respectively. Besides, comammox bacteria exhibited a high biological diversity, with clade A and clade B and three subclades of clade A all present. Among different clades, clade B played a dominant role in the ammonia oxidation process. Both the abundance and nitrification rate of comammox bacteria were significantly positively correlated with total carbon and total nitrogen, indicating that these two nutrient substances are important factors influencing this microorganism. Our results demonstrate that the spatial variations of environmental elements in the lakeshore area lead to gradual decreases of comammox bacteria from the shore to the water.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105931"},"PeriodicalIF":4.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-05DOI: 10.1016/j.ibiod.2024.105937
Getnet Belay , Carolina Suarez , Addis Simachew , Catherine J. Paul
Alkaliphilic microorganisms are one option for the treatment of cyanide-polluted wastewater. This study reports the degradation of cyanide from simulated gold mine wastewater using alkaliphilic microbial consortia harvested from a soda lake, Lake Chitu, Ethiopia. A novel aerobic-anoxic integrated treatment setup was established for the treatment process. Colorimetry was used to measure residual cyanide concentration, and 16S rRNA amplicon gene sequencing was used to study microbial diversity. This treatment system was able to degrade 97.49 ± 0.12% of 200 mg/L sodium cyanide. However, changes were observed (p < 0.05) when the established consortia were stressed with heavy metals. About 28 % of the initial inoculum persisted until the end of the treatment days. Twenty-eight bacterial phyla were identified, with Firmicutes, Proteobacteria, and Bacteroidota being the most abundant. At the end of the treatment process, Alkalibacterium (74.43%), Exaguobacterium (6.6%), and Halomonas (3.89%) were dominant. These findings indicate that alkaliphilic microbial consortiums from Ethiopian Rift Valley soda lakes are effective for the treatment of cyanide-polluted wastewater.
{"title":"A systematic evaluation of alkaliphilic microbial consortia from a soda lake for the biodegradation of cyanide-rich wastewater","authors":"Getnet Belay , Carolina Suarez , Addis Simachew , Catherine J. Paul","doi":"10.1016/j.ibiod.2024.105937","DOIUrl":"10.1016/j.ibiod.2024.105937","url":null,"abstract":"<div><div>Alkaliphilic microorganisms are one option for the treatment of cyanide-polluted wastewater. This study reports the degradation of cyanide from simulated gold mine wastewater using alkaliphilic microbial consortia harvested from a soda lake, Lake Chitu, Ethiopia. A novel aerobic-anoxic integrated treatment setup was established for the treatment process. Colorimetry was used to measure residual cyanide concentration, and 16S rRNA amplicon gene sequencing was used to study microbial diversity. This treatment system was able to degrade 97.49 ± 0.12% of 200 mg/L sodium cyanide. However, changes were observed (p < 0.05) when the established consortia were stressed with heavy metals. About 28 % of the initial inoculum persisted until the end of the treatment days. Twenty-eight bacterial phyla were identified, with Firmicutes, Proteobacteria, and Bacteroidota being the most abundant. At the end of the treatment process, <em>Alkalibacterium</em> (74.43%), <em>Exaguobacterium</em> (6.6%), and <em>Halomonas</em> (3.89%) were dominant. These findings indicate that alkaliphilic microbial consortiums from Ethiopian Rift Valley soda lakes are effective for the treatment of cyanide-polluted wastewater.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105937"},"PeriodicalIF":4.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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