Pub Date : 2025-02-01DOI: 10.1016/j.ibiod.2025.106006
Junxian Li , Zhongming Zheng , Yangcai Wang , Betina Lukwambe , Zhao Yang
The integrated aquaculture wastewater treatment system has been widely applied and typically consists of multiple units, among which macroalgae are critical for nutrients removal. This study investigated the impact of the addition of Bacillus to the macroalgae unit on the nutrient removal performance of the system and its mechanisms by analyzing the bacterial community that was identified through high-throughput sequencing of 16S rRNA. The results showed that Bacillus significantly influenced the structural composition of the bacterial communities both in the water and on the algal surface within the macroalgae unit. The enrichment of bacteria promoted nitrogen cycling and algal growth, such as Algoriphagus and Marivita. Futhermore,a higher average variation degree was observed, suggesting forming a more stable bacterial community. The removal rates of nitrogenous nutrients, such as TAN, NO3−-N, and NO2−-N, improved, reaching 99.92%, 99.62%, and 99.70%, respectively. Additionally, the biomass of the algae (U. meridionalis) significantly improved, reaching 157%. The Bacillus also increased the contribution of stochastic processes (drift) in the assembly of the bacterial communities in both the water and on the algal surface. This study provides foundational support for optimizing integrated wastewater treatment systems.
{"title":"Nutrient removal performance and microbial community analysis in an integrated wastewater treatment system with macroalgae (U. meridionalis) and Bacillus","authors":"Junxian Li , Zhongming Zheng , Yangcai Wang , Betina Lukwambe , Zhao Yang","doi":"10.1016/j.ibiod.2025.106006","DOIUrl":"10.1016/j.ibiod.2025.106006","url":null,"abstract":"<div><div>The integrated aquaculture wastewater treatment system has been widely applied and typically consists of multiple units, among which macroalgae are critical for nutrients removal. This study investigated the impact of the addition of <em>Bacillus</em> to the macroalgae unit on the nutrient removal performance of the system and its mechanisms by analyzing the bacterial community that was identified through high-throughput sequencing of 16S rRNA. The results showed that <em>Bacillus</em> significantly influenced the structural composition of the bacterial communities both in the water and on the algal surface within the macroalgae unit. The enrichment of bacteria promoted nitrogen cycling and algal growth, such as <em>Algoriphagus</em> and <em>Marivita</em>. Futhermore,a higher average variation degree was observed, suggesting forming a more stable bacterial community. The removal rates of nitrogenous nutrients, such as TAN, NO<sub>3</sub><sup>−</sup>-N, and NO<sub>2</sub><sup>−</sup>-N, improved, reaching 99.92%, 99.62%, and 99.70%, respectively. Additionally, the biomass of the algae (<em>U. meridionalis</em>) significantly improved, reaching 157%. The <em>Bacillus</em> also increased the contribution of stochastic processes (drift) in the assembly of the bacterial communities in both the water and on the algal surface. This study provides foundational support for optimizing integrated wastewater treatment systems.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106006"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.ibiod.2024.105993
L.S. Martin , S.M. Cragg , K.W. Kot , J.R. Shipway
Gribble significantly impact the biodegradation of wood in marine environments, causing costly damages, but can be used to easily assess wood consumption rates. Faecal pellet production indicates feeding rate and is a good proxy for degradation. However, counting large numbers of pellets by eye may be impractical with multiple replicates. Optimising a rapid, cost-effective, and user-friendly protocol to evaluate wood durability against gribble can be used in development of sustainable wood protection methods. Automated counting methods ImageJ and FlowCAM, were evaluated against manual counts for efficiency and accuracy. ImageJ was more suitable for large sample volumes, offering a balance of cost, time, and accuracy. Both methods showed greater degrees of error as faecal pellet counts increased. ImageJ tended to undercount, whereas FlowCAM often overcounted significantly. The FlowCAM rapidly processed individual samples, however loading/unloading samples increased time-consumption as replicates increased. ImageJ was able to process multiple samples at once so total time was not influenced by sample size. This research underlines the importance of optimising counting methods to accurately assess the impact of gribble on wood degradation, offering a streamlined approach for both specialist and non-specialist laboratories to evaluate wood durability and enhance the development of non-toxic, sustainable wood protection solutions.
{"title":"Streamlining automation in a rapid wood durability test against the marine wood boring crustacean, Limnoria quadripunctata.","authors":"L.S. Martin , S.M. Cragg , K.W. Kot , J.R. Shipway","doi":"10.1016/j.ibiod.2024.105993","DOIUrl":"10.1016/j.ibiod.2024.105993","url":null,"abstract":"<div><div>Gribble significantly impact the biodegradation of wood in marine environments, causing costly damages, but can be used to easily assess wood consumption rates. Faecal pellet production indicates feeding rate and is a good proxy for degradation. However, counting large numbers of pellets by eye may be impractical with multiple replicates. Optimising a rapid, cost-effective, and user-friendly protocol to evaluate wood durability against gribble can be used in development of sustainable wood protection methods. Automated counting methods ImageJ and FlowCAM, were evaluated against manual counts for efficiency and accuracy. ImageJ was more suitable for large sample volumes, offering a balance of cost, time, and accuracy. Both methods showed greater degrees of error as faecal pellet counts increased. ImageJ tended to undercount, whereas FlowCAM often overcounted significantly. The FlowCAM rapidly processed individual samples, however loading/unloading samples increased time-consumption as replicates increased. ImageJ was able to process multiple samples at once so total time was not influenced by sample size. This research underlines the importance of optimising counting methods to accurately assess the impact of gribble on wood degradation, offering a streamlined approach for both specialist and non-specialist laboratories to evaluate wood durability and enhance the development of non-toxic, sustainable wood protection solutions.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105993"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
To investigate how organic loading rate (OLR) affects the formation of aerobic granular sludge (AGS) in continuous flow system, the effect of OLR adjusted by hydraulic retention time (HRT) and influent chemical oxygen demand (COD) on pollutant removal, granulation efficiency and microbial community were comprehensively analyzed. The results showed that increasing OLR by decreasing HRT led to significant filamentous sludge bulking and biomass loss. Conversely, gradually increasing the OLR to 0.19 kg·(kg·d)−1 by adjusting influent COD improved the granulation efficiency from 3.00% to 28.40% with the average particle size of 193.60 μm at the 60th day. However, excessive OLR would promote filamentous bacteria to use the residual COD in the oxic tank. Organic matter utilization path analysis showed that the COD utilization enhanced by phosphate accumulating organisms (PAOs), glycogen accumulating organisms (GAOs) and adsorption (44.85 ± 2.50%) during anaerobic phase when influent COD was 350 mg L−1. And the relative abundance of PAOs and GAOs increased to 4.14% and 3.18%, respectively. This was the main cause of granulation. Furthermore, strategies to enhance granulation in continuous flow systems were proposed: Increase the volume of anaerobic tank or introduce intermittent mixing to keep more influent organics is used by bigger biomass before entering the oxic tank.
{"title":"The impact of organic loading rate on the cultivation and stability of aerobic granular sludge in continuous flow reactor","authors":"Yingjian Zhang , Fanchao Meng , Zehan Huang , Minghui Liu , Xuegang Mu , Xuelong Zhang , Guilherme Lelis Giglio , Zhaoxu Peng","doi":"10.1016/j.ibiod.2025.106016","DOIUrl":"10.1016/j.ibiod.2025.106016","url":null,"abstract":"<div><div>To investigate how organic loading rate (OLR) affects the formation of aerobic granular sludge (AGS) in continuous flow system, the effect of OLR adjusted by hydraulic retention time (HRT) and influent chemical oxygen demand (COD) on pollutant removal, granulation efficiency and microbial community were comprehensively analyzed. The results showed that increasing OLR by decreasing HRT led to significant filamentous sludge bulking and biomass loss. Conversely, gradually increasing the OLR to 0.19 kg·(kg·d)<sup>−1</sup> by adjusting influent COD improved the granulation efficiency from 3.00% to 28.40% with the average particle size of 193.60 μm at the 60th day. However, excessive OLR would promote filamentous bacteria to use the residual COD in the oxic tank. Organic matter utilization path analysis showed that the COD utilization enhanced by phosphate accumulating organisms (PAOs), glycogen accumulating organisms (GAOs) and adsorption (44.85 ± 2.50%) during anaerobic phase when influent COD was 350 mg L<sup>−1</sup>. And the relative abundance of PAOs and GAOs increased to 4.14% and 3.18%, respectively. This was the main cause of granulation. Furthermore, strategies to enhance granulation in continuous flow systems were proposed: Increase the volume of anaerobic tank or introduce intermittent mixing to keep more influent organics is used by bigger biomass before entering the oxic tank.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106016"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.ibiod.2025.106003
Khurram Shahzad , Shahid Mahmood , Azeem Khalid , Rai Muhammad Amir , Rab Nawaz , Marlia Mohd Hanfiah , Zaini Bin Sakawi , Muzammil Anjum
Freshwater resource contamination with trace metals (TMs) poses a major risk to public health and the ecosystem. In the current study, three distinct types of periphyton biofilms (PPBFs)—epiphyton, epilithon, and metaphyton—were cultured and employed to remove TMs from the simulated river water. The PPBFs were isolated from freshwater and then cultured in Bio-carrier of Organic Natural Aquatic Mate (BONAM). Based SEM analysis, the periphyton community appears porous and filamentous, with microscopic pores, unique individual cells, and extracellular matrix. Even while each PPBF has a unique structure and function, they all share rich active surface functional groups, particularly carboxylic groups. The epiphyton was found to be more effective for the removal of TMs when tested at 20 mg/L of the TMs as evidenced by the 79.50% elimination of arsenic (As) following 144 h of treatment. The removal performance of epiphyton decreased to just 57.40 and 51.60% at TMs concentration of 40 and 60 mg/L, respectively. Temperature had a significant impact on the biosorption of TMs utilising epiphyton, as evidenced by the removal efficiency of 75.05% at 15 °C, which decreased to 69.50 and 61.00% at 25 °C and 35 °C, respectively. The findings pertaining to the removal of TMs most accurately represented pseudo-first-order kinetics, indicating the bio-absorption of the TMs into the PPBFs. The elimination of almost 92% of TMs under optimal conditions of 90 h of incubation at neutral pH, 25 °C, 1.0 g L−1 of biomass, and 20 mg/L of TMs concentration demonstrated that epiphyton was the most effective biomaterial.
{"title":"Periphyton biofilms formulation and application for the removal of trace pollutants from water","authors":"Khurram Shahzad , Shahid Mahmood , Azeem Khalid , Rai Muhammad Amir , Rab Nawaz , Marlia Mohd Hanfiah , Zaini Bin Sakawi , Muzammil Anjum","doi":"10.1016/j.ibiod.2025.106003","DOIUrl":"10.1016/j.ibiod.2025.106003","url":null,"abstract":"<div><div>Freshwater resource contamination with trace metals (TMs) poses a major risk to public health and the ecosystem. In the current study, three distinct types of periphyton biofilms (PPBFs)—epiphyton, epilithon, and metaphyton—were cultured and employed to remove TMs from the simulated river water. The PPBFs were isolated from freshwater and then cultured in Bio-carrier of Organic Natural Aquatic Mate (BONAM). Based SEM analysis, the periphyton community appears porous and filamentous, with microscopic pores, unique individual cells, and extracellular matrix. Even while each PPBF has a unique structure and function, they all share rich active surface functional groups, particularly carboxylic groups. The epiphyton was found to be more effective for the removal of TMs when tested at 20 mg/L of the TMs as evidenced by the 79.50% elimination of arsenic (As) following 144 h of treatment. The removal performance of epiphyton decreased to just 57.40 and 51.60% at TMs concentration of 40 and 60 mg/L, respectively. Temperature had a significant impact on the biosorption of TMs utilising epiphyton, as evidenced by the removal efficiency of 75.05% at 15 °C, which decreased to 69.50 and 61.00% at 25 °C and 35 °C, respectively. The findings pertaining to the removal of TMs most accurately represented pseudo-first-order kinetics, indicating the bio-absorption of the TMs into the PPBFs. The elimination of almost 92% of TMs under optimal conditions of 90 h of incubation at neutral pH, 25 °C, 1.0 g L<sup>−1</sup> of biomass, and 20 mg/L of TMs concentration demonstrated that epiphyton was the most effective biomaterial.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106003"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.ibiod.2025.105998
Shuang Zhao , Yi Zhang , Rongjiu Shi , Xiaolong Liang , Ping Li , Xue Bai , Siqin Han , Ying Zhang
The prolonged and widespread use of veterinary antibiotics resulted in a significant accumulation of antibiotic residues in the soil surrounding poultry farms, thereby promoting the proliferation and dissemination of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB). In the northern regions of China, low temperatures hinder the microbial degradation of antibiotics. This study reports that Acinetobacter sp. H-3, isolated from a poultry farm in Liaoning Province, Northeast China, is capable of effectively degrading SDZ at a minimum temperature of 5°C, with optimal degradation efficiency observed at 15°C. The strain H-3 removed 82% of SDZ from the soil over a 14-day period at 15°C, with 53% of this degradation directly attributed to the activity of strain H-3. High-throughput sequencing and RT-qPCR analyses revealed that exposure to SDZ significantly altered the soil bacterial community structure, inhibited soil microbial functions and increased the abundance of sul1, sul2 and intI1 genes. Following the application of, certain bacterial genera in the contaminated soil exhibited indications of recovery. However, the overall soil microbial function did not show a recovery trend. Additionally, the presence of strain H-3, which carries the sul1, sul2 and intI1 genes, resulted in an increase in sul1, followed by a subsequent decrease, while the levels of sul2 and intI1 continued to rise. This study is the first to report that Acinetobacter sp. strain H-3 can effectively remove SDZ at low temperatures. Furthermore, it provides a preliminary evaluation of the ecological risks linked to the dissemination of ARGs during the application of this strain, contributing valuable resources and theoretical insights for the bioremediation of antibiotic-polluted soil in cold areas.
{"title":"Sulfadiazine degradation by Acinetobacter sp. strain H-3 and its applicability in soil at low temperatures","authors":"Shuang Zhao , Yi Zhang , Rongjiu Shi , Xiaolong Liang , Ping Li , Xue Bai , Siqin Han , Ying Zhang","doi":"10.1016/j.ibiod.2025.105998","DOIUrl":"10.1016/j.ibiod.2025.105998","url":null,"abstract":"<div><div>The prolonged and widespread use of veterinary antibiotics resulted in a significant accumulation of antibiotic residues in the soil surrounding poultry farms, thereby promoting the proliferation and dissemination of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB). In the northern regions of China, low temperatures hinder the microbial degradation of antibiotics. This study reports that <em>Acinetobacter</em> sp. H-3, isolated from a poultry farm in Liaoning Province, Northeast China, is capable of effectively degrading SDZ at a minimum temperature of 5°C, with optimal degradation efficiency observed at 15°C. The strain H-3 removed 82% of SDZ from the soil over a 14-day period at 15°C, with 53% of this degradation directly attributed to the activity of strain H-3. High-throughput sequencing and RT-qPCR analyses revealed that exposure to SDZ significantly altered the soil bacterial community structure, inhibited soil microbial functions and increased the abundance of <em>sul1</em>, <em>sul2</em> and <em>intI1</em> genes. Following the application of, certain bacterial genera in the contaminated soil exhibited indications of recovery. However, the overall soil microbial function did not show a recovery trend. Additionally, the presence of strain H-3, which carries the <em>sul1</em>, <em>sul2</em> and <em>intI1</em> genes, resulted in an increase in <em>sul1</em>, followed by a subsequent decrease, while the levels of <em>sul2</em> and <em>intI1</em> continued to rise. This study is the first to report that <em>Acinetobacter</em> sp. strain H-3 can effectively remove SDZ at low temperatures. Furthermore, it provides a preliminary evaluation of the ecological risks linked to the dissemination of ARGs during the application of this strain, contributing valuable resources and theoretical insights for the bioremediation of antibiotic-polluted soil in cold areas.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105998"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.ibiod.2025.105999
Lu Xu , Yinhu Jiang , Jiale Ma , Kexin Wang , Ying Zhou , Qimiao Xu , Jiguo Qiu , Jiandong Jiang , Jian He , Zhuang Ke
Chloroxylenol (synonym, para-chloro-meta-xylenol [PCMX]), a halogenated phenolic disinfectant, is widely used for disinfection or in personal care products. Due to the global COVID-19 pandemic, the widespread use of PCMX has undoubtedly led to its prevalence in various environments, resulting in a high detection frequency. However, there have been few reports on the aerobic microbial community and pure culture degradation of PCMX. In this study, a PC2 consortium with an efficient PCMX decomposition was successfully obtained. Rhodococcus was significantly enriched in consortium PC2 after the acclimation. Subsequently, a PCMX-degrading pure culture strain, Rhodococcus sp. JH-7, was isolated, and the degradation characteristics were investigated. Meanwhile, a key metabolic intermediate (4-chloro-3,5-dimethylcatechol) was identified by LC-TOF-MS and a new biodegradation pathway of PCMX was proposed. Additionally, Rhodococcus sp. JH-7 could protect Escherichia coli, Pichia pastoris, Chlorella ellipsoidea and Chlamydomonas reinhardtii from the toxicity of PCMX, indicating that the biodegradation of PCMX was a detoxification process. This study reveals a catabolic pathway of PCMX and provides new insights for the bioremediation of PCMX-contaminated environments.
{"title":"Functional characterization of an efficient chloroxylenol-degrading bacterial consortium","authors":"Lu Xu , Yinhu Jiang , Jiale Ma , Kexin Wang , Ying Zhou , Qimiao Xu , Jiguo Qiu , Jiandong Jiang , Jian He , Zhuang Ke","doi":"10.1016/j.ibiod.2025.105999","DOIUrl":"10.1016/j.ibiod.2025.105999","url":null,"abstract":"<div><div>Chloroxylenol (synonym, para-chloro-meta-xylenol [PCMX]), a halogenated phenolic disinfectant, is widely used for disinfection or in personal care products. Due to the global COVID-19 pandemic, the widespread use of PCMX has undoubtedly led to its prevalence in various environments, resulting in a high detection frequency. However, there have been few reports on the aerobic microbial community and pure culture degradation of PCMX. In this study, a PC2 consortium with an efficient PCMX decomposition was successfully obtained. <em>Rhodococcus</em> was significantly enriched in consortium PC2 after the acclimation. Subsequently, a PCMX-degrading pure culture strain, <em>Rhodococcus</em> sp. JH-7, was isolated, and the degradation characteristics were investigated. Meanwhile, a key metabolic intermediate (4-chloro-3,5-dimethylcatechol) was identified by LC-TOF-MS and a new biodegradation pathway of PCMX was proposed. Additionally, <em>Rhodococcus</em> sp. JH-7 could protect <em>Escherichia coli</em>, <em>Pichia pastoris</em>, <em>Chlorella ellipsoidea</em> and <em>Chlamydomonas reinhardtii</em> from the toxicity of PCMX, indicating that the biodegradation of PCMX was a detoxification process. This study reveals a catabolic pathway of PCMX and provides new insights for the bioremediation of PCMX-contaminated environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 105999"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.ibiod.2025.106011
Chun-Chin Wang , Chih-Ming Liang , Ting-I Lin , Chu-Fang Yang
Chlorinated ethylenes (CEs) are widely used in various industries, making them common groundwater pollutants. The reductive dechlorination applied to deal with CEs has the drawback of lower-chlorinated CEs accumulation. Aerobic co-metabolism relying on methanotrophs is an attractive strategy to deal with CEs because of its fast CE degrading rate and no lower-chlorinated CEs accumulation. In this study, one methanotrophic consortium was acclimated with methane and TCE concentrations for five phases. The bacterial community and methane monooxygenase (MMO) functional genes were analyzed during enrichment. The methane gas and micro-nano bubbles (MNBs) were applied to perform batch experiments using the acclimated methanotrophic consortium as the inoculum. The results indicated that a methanotrophic consortium M-5%-0.2 was successfully acclimated. Genus Methylocystis was dominant in the acclimated consortium M-5%-0.2, and the particulate methane monooxygenase gene (pmoA) could be detected. With different CEs combinations and 5% methane gas, 0.2 and 0.3 mg/L cis-1,2-DCE and VC could be entirely removed. TCE removal efficiencies were 38.8% and 40.2%, as the TCE concentrations were 0.2 and 0.3 mg/L, respectively. CEs removal pattern was similar in the presence of various methane MNBs ratios. The priority order of CEs removal was VC > cis-1,2-DCE > TCE. The degradation efficiencies of 0.2 mg/L TCE, cis-1,2-DCE, and VC were 17.4%, 78.4%, and 100% when adding 24% methane MNBs water as the carbon source. The methanotrophic consortium M-5%-0.2 could utilize the methane MNBs having a diameter larger than 235 nm. In conclusion, the aerobic co-metabolism strategy using methane MNBs has the potential to be applied for CEs bioremediation.
{"title":"Enrichment of methanotrophic consortium and its aerobic cometabolism of mixed vinyl chloride pollutants using methane gas and micro- and nano-bubbles","authors":"Chun-Chin Wang , Chih-Ming Liang , Ting-I Lin , Chu-Fang Yang","doi":"10.1016/j.ibiod.2025.106011","DOIUrl":"10.1016/j.ibiod.2025.106011","url":null,"abstract":"<div><div>Chlorinated ethylenes (CEs) are widely used in various industries, making them common groundwater pollutants. The reductive dechlorination applied to deal with CEs has the drawback of lower-chlorinated CEs accumulation. Aerobic co-metabolism relying on methanotrophs is an attractive strategy to deal with CEs because of its fast CE degrading rate and no lower-chlorinated CEs accumulation. In this study, one methanotrophic consortium was acclimated with methane and TCE concentrations for five phases. The bacterial community and methane monooxygenase (MMO) functional genes were analyzed during enrichment. The methane gas and micro-nano bubbles (MNBs) were applied to perform batch experiments using the acclimated methanotrophic consortium as the inoculum. The results indicated that a methanotrophic consortium M-5%-0.2 was successfully acclimated. Genus <em>Methylocystis</em> was dominant in the acclimated consortium M-5%-0.2, and the particulate methane monooxygenase gene (<em>pmoA</em>) could be detected. With different CEs combinations and 5% methane gas, 0.2 and 0.3 mg/L cis-1,2-DCE and VC could be entirely removed. TCE removal efficiencies were 38.8% and 40.2%, as the TCE concentrations were 0.2 and 0.3 mg/L, respectively. CEs removal pattern was similar in the presence of various methane MNBs ratios. The priority order of CEs removal was VC > cis-1,2-DCE > TCE. The degradation efficiencies of 0.2 mg/L TCE, cis-1,2-DCE, and VC were 17.4%, 78.4%, and 100% when adding 24% methane MNBs water as the carbon source. The methanotrophic consortium M-5%-0.2 could utilize the methane MNBs having a diameter larger than 235 nm. In conclusion, the aerobic co-metabolism strategy using methane MNBs has the potential to be applied for CEs bioremediation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106011"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.ibiod.2025.106015
Lingyue Deng , Yongsen Wang , Qiaoyan Wei , Xiaojin Guan , Quanzeng Li , Yu Pan , Kehui Liu , Zongbao Liu
Our previous studies have demonstrated that sulfate can enhance the accumulation of manganese (Mn) in Polygonum lapathifolium Linn (P. lapathifolium L.). However, the underlying mechanisms, particularly the regulatory mechanisms at the metabolic level, remain unclear. This study systematically investigates the mechanism of sulfate on Mn accumulation in P. lapathifolium L. using metabolomics. We analyzed the plant's growth characteristics, Mn accumulation, photosynthetic pigment content, and metabolite composition after 55 days of cultivation. The results show that Mn treatment increased plant weight and root length, decreased plant height, and increased lateral branches significantly (P < 0.05). Sulfate addition further influenced growth indicators, suggesting a complex role in plant growth regulation. Mn accumulation was highest in leaves, and sulfate addition significantly increased Mn concentrations in all plant parts. Bioconcentration and translocation factor analyses showed improved Mn translocation from roots to above-ground parts with sulfate treatment. PCA and OPLS-DA revealed significant differences in metabolite composition between treatment groups, notably in flavonoids, organooxygen compounds, and carboxylic acids and derivatives. KEGG enrichment analysis indicated significant enrichment of flavonoid biosynthesis, propanoate metabolism, and ABC transporters, suggesting their crucial role in Mn stress response. Mantel Test revealed significant correlations between environmental factors and metabolites, with available potassium (AK), available phosphorus (AP), and soil Mn significantly influencing key metabolite synthesis. These findings enhance the understanding of P. lapathifolium L.‘s metabolic response to Mn stress, showing that sulfate enhances Mn tolerance and remediation by regulating metabolite synthesis and transport, providing a basis for improved phytoremediation techniques.
{"title":"Metabolomic insights into sulfate-enhanced manganese remediation in Polygonum lapathifolium Linn","authors":"Lingyue Deng , Yongsen Wang , Qiaoyan Wei , Xiaojin Guan , Quanzeng Li , Yu Pan , Kehui Liu , Zongbao Liu","doi":"10.1016/j.ibiod.2025.106015","DOIUrl":"10.1016/j.ibiod.2025.106015","url":null,"abstract":"<div><div>Our previous studies have demonstrated that sulfate can enhance the accumulation of manganese (Mn) in <em>Polygonum lapathifolium</em> Linn (<em>P. lapathifolium</em> L.). However, the underlying mechanisms, particularly the regulatory mechanisms at the metabolic level, remain unclear. This study systematically investigates the mechanism of sulfate on Mn accumulation in <em>P. lapathifolium</em> L. using metabolomics. We analyzed the plant's growth characteristics, Mn accumulation, photosynthetic pigment content, and metabolite composition after 55 days of cultivation. The results show that Mn treatment increased plant weight and root length, decreased plant height, and increased lateral branches significantly (<em>P</em> < 0.05). Sulfate addition further influenced growth indicators, suggesting a complex role in plant growth regulation. Mn accumulation was highest in leaves, and sulfate addition significantly increased Mn concentrations in all plant parts. Bioconcentration and translocation factor analyses showed improved Mn translocation from roots to above-ground parts with sulfate treatment. PCA and OPLS-DA revealed significant differences in metabolite composition between treatment groups, notably in flavonoids, organooxygen compounds, and carboxylic acids and derivatives. KEGG enrichment analysis indicated significant enrichment of flavonoid biosynthesis, propanoate metabolism, and ABC transporters, suggesting their crucial role in Mn stress response. Mantel Test revealed significant correlations between environmental factors and metabolites, with available potassium (AK), available phosphorus (AP), and soil Mn significantly influencing key metabolite synthesis. These findings enhance the understanding of <em>P. lapathifolium</em> L.‘s metabolic response to Mn stress, showing that sulfate enhances Mn tolerance and remediation by regulating metabolite synthesis and transport, providing a basis for improved phytoremediation techniques.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106015"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.ibiod.2025.106010
Takayuki Kakuda , Ayana Suzuki , Rin Naito , Kanta Tominaga , Akira Hafuka , Hiroshi Yamamura , Katsuki Kimura
Biopolymers have been widely recognized as playing an important role in membrane fouling in membrane bioreactors (MBRs). In-depth characterization of biopolymers in MBRs can provide important insights into membrane fouling. In this study, ultrafiltration was used to selectively isolate biopolymers (recovery rate: 70%) from a pilot-scale MBR treating municipal wastewater. The characteristics of the isolated biopolymers were compared against those of model substances, such as sodium alginate (SA) and bovine serum albumin (BSA), which have been used in many previous studies. Lipopolysaccharides (LPS), which have been proposed as alternative model polysaccharides for MBR fouling research, were also characterized. Batch filtration tests demonstrated that the isolated biopolymers had a considerably higher fouling potential than the model substances. A modified liquid chromatography with organic carbon detection (LC–OCD) analysis showed that the constituents of the isolated biopolymers had molecular weights of >1,000,000 Da. Such macromolecular constituents were only detected in LPS among the model substances. A Fourier-transform infrared (FTIR) analysis indicated various constituents for the isolated biopolymers, such as polysaccharides, proteins and lipids. A quartz crystal microbalance (QCM) analysis demonstrated that the isolated biopolymers had a high affinity to polyvinylidene fluoride (PVDF), explaining the high fouling potential of these biopolymers.
{"title":"Characterization of biopolymers isolated from a pilot-scale membrane bioreactor treating municipal wastewater","authors":"Takayuki Kakuda , Ayana Suzuki , Rin Naito , Kanta Tominaga , Akira Hafuka , Hiroshi Yamamura , Katsuki Kimura","doi":"10.1016/j.ibiod.2025.106010","DOIUrl":"10.1016/j.ibiod.2025.106010","url":null,"abstract":"<div><div>Biopolymers have been widely recognized as playing an important role in membrane fouling in membrane bioreactors (MBRs). In-depth characterization of biopolymers in MBRs can provide important insights into membrane fouling. In this study, ultrafiltration was used to selectively isolate biopolymers (recovery rate: 70%) from a pilot-scale MBR treating municipal wastewater. The characteristics of the isolated biopolymers were compared against those of model substances, such as sodium alginate (SA) and bovine serum albumin (BSA), which have been used in many previous studies. Lipopolysaccharides (LPS), which have been proposed as alternative model polysaccharides for MBR fouling research, were also characterized. Batch filtration tests demonstrated that the isolated biopolymers had a considerably higher fouling potential than the model substances. A modified liquid chromatography with organic carbon detection (LC–OCD) analysis showed that the constituents of the isolated biopolymers had molecular weights of >1,000,000 Da. Such macromolecular constituents were only detected in LPS among the model substances. A Fourier-transform infrared (FTIR) analysis indicated various constituents for the isolated biopolymers, such as polysaccharides, proteins and lipids. A quartz crystal microbalance (QCM) analysis demonstrated that the isolated biopolymers had a high affinity to polyvinylidene fluoride (PVDF), explaining the high fouling potential of these biopolymers.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106010"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-02-01DOI: 10.1016/j.ibiod.2025.106008
Carlos Esteven Pulgarín-Muñoz , Julio César Saldarriaga-Molina , Johan Camilo Castro-Valencia , Mauricio Andres Correa-Ochoa , Juan David Echeverry-Ruiz
The anaerobic co-digestion (ACoD) of sewage sludge (SS) and the organic fraction of municipal solid waste (OFMSW) has become a viable strategy for waste management, contributing to renewable energy production in the form of biogas and reducing environmental impact. This study aimed to evaluate the co-digestion process of these wastes at pilot scale, assessing three co-substrate ratios of 75/25 (R1-1), 50/50 (R2-1), and 25/75 (R3-1) of OFMSW and SS, respectively. The results indicated that the 75/25 ratio generated the highest methane gas yield, reaching 992.5 mL CH₄/gVS, and showed a greater rate of organic matter degradation, volatile solids removal reached 53.75%. The degradation of pathogenic microorganisms was effective in all reactors, meeting the requirements for producing digestate with potential use as fertilizer. The applied kinetic models allowed for high precision fitting of experimental data, with determination coefficients (R2) exceeding 0.99, confirming their ability to predict biogas production across different configurations. This study demonstrates that the ACoD of OFMSW and SS is an efficient solution for waste management and bioenergy production, generating a digestate suitable for agricultural use. The data provided at pilot scale are closer to real conditions and offer a better understanding for its implementation at full scale.
{"title":"Effect of the co-substrate ratio on the anaerobic co-digestion of sewage sludge and the organic fraction of municipal solid waste at pilot scale","authors":"Carlos Esteven Pulgarín-Muñoz , Julio César Saldarriaga-Molina , Johan Camilo Castro-Valencia , Mauricio Andres Correa-Ochoa , Juan David Echeverry-Ruiz","doi":"10.1016/j.ibiod.2025.106008","DOIUrl":"10.1016/j.ibiod.2025.106008","url":null,"abstract":"<div><div>The anaerobic co-digestion (ACoD) of sewage sludge (SS) and the organic fraction of municipal solid waste (OFMSW) has become a viable strategy for waste management, contributing to renewable energy production in the form of biogas and reducing environmental impact. This study aimed to evaluate the co-digestion process of these wastes at pilot scale, assessing three co-substrate ratios of 75/25 (R1-1), 50/50 (R2-1), and 25/75 (R3-1) of OFMSW and SS, respectively. The results indicated that the 75/25 ratio generated the highest methane gas yield, reaching 992.5 mL CH₄/gVS, and showed a greater rate of organic matter degradation, volatile solids removal reached 53.75%. The degradation of pathogenic microorganisms was effective in all reactors, meeting the requirements for producing digestate with potential use as fertilizer. The applied kinetic models allowed for high precision fitting of experimental data, with determination coefficients (R<sup>2</sup>) exceeding 0.99, confirming their ability to predict biogas production across different configurations. This study demonstrates that the ACoD of OFMSW and SS is an efficient solution for waste management and bioenergy production, generating a digestate suitable for agricultural use. The data provided at pilot scale are closer to real conditions and offer a better understanding for its implementation at full scale.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"198 ","pages":"Article 106008"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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