International Biodeterioration & Biodegradation最新文献_第2页

Biological treatment of sulfate-laden acidic wastewater in a semi-batch reactor: Performance optimization and microbial community dynamics 半间歇反应器处理含硫酸盐酸性废水：性能优化和微生物群落动态

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2026-01-03 DOI: 10.1016/j.ibiod.2026.106276

Sreekanth Yadav Golla , Guntakala Venkatanaga Chandra , Pranab Kumar Ghosh

Sulfate-laden acidic wastewater generated from industries and mining activities is a global concern due to its adverse effects. To address this challenge, a bench-scale sulfidogenic semi-batch reactor (SmBR) inoculated with mixed bacterial culture was operated for 281 days to evaluate the effect of sulfate (500–4500 mg/L) and acidic conditions (pH 6.0-1.85) on sulfate removal and microbial communities. The optimum

COD / {SO}_{4}^{2 ‐}

ratio and HRT were found to be 0.7 and 4 days, respectively. The SmBR achieved a sulfate and COD removal of 80 % and 87 %, respectively, with an influent sulfate concentration of 4500 mg/L and a

COD / {SO}_{4}^{2 ‐}

ratio of 0.7, and the effluent pH increased from 2.0 to 7.2. Further decreasing the feed pH to 1.85 resulted in a sharp decline in the reactor performance, which was recovered within 30 days by adjusting operational conditions. Electron flow from lactate to sulfate reduction varied between 86 % and 91 %, demonstrating the effective utilization of substrate. 16S rRNA sequencing revealed that the decrease in influent pH from 6.0 to 2.5 led to a shift in microbial diversity towards a specialized group of SRB, particularly Desulfobacterota (Desulfovibrio genus), which increased from 12 to 45 %, emphasizing their crucial role in sulfate removal. The mixed bacterial culture developed in the SmBR could be suitable for treating industrial sulfate-laden acidic wastewaters such as acid mine drainage, lead acid battery manufacturing wastewater, and secondary lead smelting plant wastewater. The findings of this study offer a sustainable bioremediation approach for treating sulfate-laden acidic wastewater.

工业和采矿活动产生的含硫酸盐酸性废水因其不利影响而受到全球关注。为了解决这一问题，我们在实验规模的硫化半间歇反应器（SmBR）上接种了混合细菌培养物，运行了281天，以评估硫酸盐（500-4500 mg/L）和酸性条件（pH 6.0-1.85）对硫酸盐去除和微生物群落的影响。最佳COD/SO42‐比和HRT分别为0.7和4 d。SmBR在进水硫酸盐浓度为4500 mg/L、COD/SO42 -比值为0.7的情况下，硫酸盐和COD去除率分别为80%和87%，出水pH从2.0提高到7.2。进一步降低进料pH至1.85，导致反应器性能急剧下降，通过调整操作条件，在30天内恢复。乳酸还原到硫酸盐还原的电子流在86% ~ 91%之间变化，表明底物的有效利用。16S rRNA测序显示，进水pH从6.0降至2.5导致微生物多样性向SRB的一个特殊群体转移，特别是Desulfovibrio属（desulfoobacterota），从12%增加到45%，强调了它们在硫酸盐去除中的关键作用。在SmBR中开发的混合细菌培养物可用于处理酸性矿山废水、铅酸蓄电池生产废水、二次炼铅厂废水等含硫酸盐的工业酸性废水。本研究结果为处理含硫酸盐酸性废水提供了一种可持续的生物修复方法。

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引用次数: 0

Biofilm-mediated mitigation of nitrous oxide emissions in anammox-based wastewater treatment 生物膜介导的厌氧氨氧化废水处理中氧化亚氮排放的减缓

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-12-31 DOI: 10.1016/j.ibiod.2025.106274

Zhiman Lin , Jinfan Zhang , Ru Wang , Jingmin Ou , He Zhang , Zihua Wang , Fangyuan Zheng , Xiantao Sun , Xin Wei , Yiming Huang , Zhenguo Chen , Mark C.M. van Loosdrecht , Yuchun Yang

Wastewater treatment is a major source of global greenhouse gas (GHG) emissions, largely driven by nitrous oxide (N₂O) release during nitrogen removal processes. While anaerobic ammonium-oxidizing (anammox) bacteria have become widely adopted to optimize nitrogen removal, the pathways governing N₂O emissions in anammox systems remain insufficiently characterized. Findings revealed that in aerobic environments with low dissolved oxygen, nitrifying microorganisms showed pronounced upregulation of N₂O synthesis genes, confirming their pivotal role in emissions. Conversely, heterotrophic denitrification dominated N₂O production in anaerobic systems. Crucially, anammox-dominated biofilms displayed substantially lower expression of N₂O-related genes compared to suspended sludge, suggesting reduced emission risks in biofilm configurations. Batch experiments demonstrated a 2.78-fold lower N₂O release from biofilms than suspended biomass. This mitigation was linked to restricted substrate transport within biofilms, which curbed NO₂⁻ buildup and subsequently suppressed nitrifier-derived N₂O formation. Additionally, the high-density colonization of anammox bacteria in biofilms efficient scavenging of nitric oxide (NO) and hydroxylamine (NH₂OH), critical intermediates in N₂O synthesis. Employing an integrated multi-omics approach, this study investigates N₂O production and consumption pathways in diverse anammox systems and unveils the N₂O mitigation mechanisms of biofilms, offering fundamental insights for reducing N₂O emissions in wastewater treatment.

废水处理是全球温室气体（GHG）排放的主要来源，主要由氮去除过程中氧化亚氮（N2O）的释放驱动。虽然厌氧氨氧化（anammox）细菌已被广泛应用于优化氮去除，但在厌氧氨氧化系统中控制N2O排放的途径仍然缺乏充分的表征。研究结果表明，在低溶解氧的好氧环境中，硝化微生物的N2O合成基因明显上调，证实了它们在排放中的关键作用。相反，异养反硝化作用在厌氧系统中主导N2O的产生。关键是，厌氧氨氧化主导的生物膜与悬浮污泥相比，n2o相关基因的表达显著降低，这表明生物膜配置降低了排放风险。批量实验表明，生物膜的N2O释放量比悬浮生物质低2.78倍。这种缓解与生物膜内受限的底物运输有关，这抑制了NO2−的积累，随后抑制了硝化菌衍生的N2O的形成。此外，厌氧氨氧化菌在生物膜中的高密度定植可以有效清除一氧化氮（NO）和羟胺（NH2OH），这是合成N2O的关键中间体。本研究采用综合多组学方法，研究了不同厌氧氨氧化系统中N2O的产生和消耗途径，揭示了生物膜的N2O减缓机制，为减少废水处理中N2O的排放提供了基础见解。

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引用次数: 0

Exploring calcium carbonate biomineralization by yeasts isolated from cement-associated environments 从水泥相关环境中分离的酵母探索碳酸钙生物矿化

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-12-31 DOI: 10.1016/j.ibiod.2025.106275

Giovana Manzini , Giuseppe Ciaramella Moita , Romildo Dias Toledo Filho , Eliana Flávia Camporese Sérvulo , Vitor Liduino

This study advances microbial-induced calcium carbonate precipitation (MICP), a technology with potential to mitigate carbon emissions. A composite mixture of natural and recycled stone aggregates was tested for the isolation of carbonate-forming fungi, seeking to explore alternative eukaryotic microorganisms capable of MICP. Three yeast and seven mold cultures were isolated. The yeast isolates, identified through ITS sequencing as Candida orthopsilosis, Naganishia sp., and Rhodotorula mucilaginosa, were evaluated for their ability to produce CaCO₃ in an acetate-based medium over a 7-day cultivation period. All three yeasts generated mineral residues, showing different overall yields (ranging from 0.6 to 1.6 g) but with similar carbonate contents (∼20 % as CaCO₃). Fourier-transform infrared spectroscopy (FTIR) confirmed the presence of carbonate groups in all mineralized residues. At the same time, thermogravimetric (TG-DTG) analyses revealed that among the isolates, R. mucilaginosa achieved the highest conversion rate of calcium acetate to CaCO₃ (48.8 ± 3.1 %), corresponding to a precipitation yield of 0.29 g CaCO₃, more than twice that obtained for the other isolates. X-ray diffraction (XRD) analyses revealed that the only CaCO₃ polymorph formed was vaterite. These findings provide new insights into the biomineralization capacity of non-ureolytic yeasts and highlight their potential applicability as alternative biocatalysts in MICP-based technologies.

这项研究推进了微生物诱导碳酸钙沉淀（MICP）技术，这是一项有潜力减少碳排放的技术。测试了天然和再生石材骨料的复合混合物，以分离碳酸盐形成真菌，寻求探索能够MICP的替代真核微生物。分离出3个酵母培养物和7个霉菌培养物。通过ITS测序鉴定，分离的酵母菌分别为念珠菌（Candida orthopsilosis）、Naganishia sp.和粘液红酵母（Rhodotorula mucilaginosa），并对它们在醋酸盐培养基中产生CaCO3的能力进行了评估，培养周期为7天。所有三种酵母产生的矿物残留物，显示出不同的总产量（从0.6到1.6 g不等），但碳酸盐含量相似（约为CaCO3的20%）。傅里叶变换红外光谱（FTIR）证实了所有矿化残留物中碳酸盐基团的存在。同时，热重分析（TG-DTG）结果表明，在所有菌株中，粘胶菌的醋酸钙转化率最高（48.8±3.1%），相当于CaCO3的沉淀产率为0.29 g，是其他菌株的2倍以上。x射线衍射（XRD）分析表明，CaCO3形成的唯一多晶是水晶石。这些发现为非解尿酵母菌的生物矿化能力提供了新的见解，并强调了它们作为基于micp技术的替代生物催化剂的潜在适用性。

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引用次数: 0

Study on the effect of ethylene oxide fumigation on the colonies of unearthed wooden cultural relics 环氧乙烷熏蒸对出土木质文物菌落影响的研究

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-12-30 DOI: 10.1016/j.ibiod.2025.106273

Hui Li , Zeyu Pan , Chuan Yang , Meng Sun , Hui Jiang , Buyu Cao , Xingxia Ma

This study aimed to evaluate the disinfection effectiveness of ethylene oxide fumigation (EOF) on unearthed wooden cultural relics, with offering practical references for the selection of more comprehensive protection strategies for wood. Two ancient wooden wells from the Beijing Lu County Gucheng ruins park were treated with EOF. Wood species were identified, and rotten wood was observed microscopically. Microbial communities were analyzed through tissue isolation and high-throughput sequencing. Growth characteristics, lignocellulose-degrading enzyme activities, and microbial interactions of isolated strains were further assessed. Enzymatic analyses showed that most isolates lacked ligninolytic activity but exhibited cellulase activity, consistent with soft-rot patterns observed in the wooden wells. Before fumigation, fourteen fungal species were isolated, with Exophiala and Anthracobia dominating. After EOF treatment, only five fungal species were isolated, indicating a substantial reduction in microbial diversity. EOF reduced surface fungal loads but did not fully eliminate deeply invasive strains. These findings indicate the limited sterilization capacity of EOF for archaeological wood, for which improved or complementary disinfection strategies may be required to ensure the long-term preservation of deeply deteriorated wooden cultural relics.

本研究旨在评价环氧乙烷熏蒸（EOF）对出土木质文物的消毒效果，为选择更全面的木质文物保护策略提供实用参考。采用EOF对北京鲁县古城遗址公园的两口古木井进行了处理。鉴定了木材种类，并对腐木进行了显微观察。通过组织分离和高通量测序分析微生物群落。进一步评估分离菌株的生长特性、木质纤维素降解酶活性和微生物相互作用。酶分析表明，大多数菌株缺乏木质素降解活性，但具有纤维素酶活性，这与在木井中观察到的软腐病模式一致。熏蒸前分离到14种真菌，以外菌属和炭疽菌属为主。经EOF处理后，仅分离出5种真菌，表明微生物多样性大幅降低。EOF降低了表面真菌负荷，但不能完全消除深度侵入菌株。这些结果表明，EOF对考古木材的消毒能力有限，因此可能需要改进或补充消毒策略，以确保严重变质的木制文物的长期保存。

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引用次数: 0

Quorum sensing inhibitory and anti-infective potential of piperonyl alcohol against Pseudomonas aeruginosa 胡椒醇对铜绿假单胞菌的群体感应抑制及抗感染潜力

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-12-27 DOI: 10.1016/j.ibiod.2025.106272

Paramita Pakhira , Paramanantham Parasuraman , Sampathkumar Ranganathan , Jung-Kul Lee , Mahesh Ramatchandirane , Kitlangki Suchiang , Siddhardha Busi

Pseudomonas aeruginosa is a notorious opportunistic pathogenic Gram-negative bacterium, and its virulence and persistence are mainly facilitated by quorum sensing (QS) and biofilm formation. Targeting these mechanisms presents a promising strategy to reduce pathogenicity without exerting selective pressure on bacterial survival. This study examined the anti-virulence potential of Piperonyl alcohol (PAL) against P. aeruginosa PAO1 using both experimental and computational approaches. Our findings revealed that PAL treatment notable decreased the production of key virulence factors, including pyocyanin (72.66 %), total protease (74.66 %), alginate (66.95 %), exopolysaccharides (79.14 %), pyoverdine (79.92 %), and pyochelin (68.28 %). Additionally, biofilm formation was reduced by 83.71 %, which was further validated through catheter-based biofilm assays and microscopic analyses. PAL also impaired swimming, swarming, and twitching motilities, indicating its interference in the initial biofilm formation. Gene expression profiling confirmed the downregulation of major QS regulators (lasR, rhlR, pqsR) and related virulence genes. In silico studies, revealed stable binding of PAL to LasR, RhlR, and PqsR as evidenced by favorable docking scores and corroborated by molecular dynamics simulations. Pharmacokinetic predictions indicated good oral bioavailability and intestinal absorption with no apparent toxicity. In vivo validation using infection models in C. elegans and G. mellonella demonstrated enhanced host survival and reduced pathogen burden. These findings position PAL as a potential QS and biofilm inhibitory agent, with substantial potential for therapeutic management of P. aeruginosa infections.

铜绿假单胞菌是一种臭名昭著的机会致病性革兰氏阴性菌，其毒力和持久性主要是通过群体感应（quorum sensing， QS）和生物膜的形成来促进的。针对这些机制提出了一个有希望的策略，以减少致病性而不施加选择性压力的细菌生存。本研究采用实验和计算两种方法检测胡椒醇（PAL）对铜绿假单胞菌PAO1的抗毒潜力。结果表明，PAL处理显著降低了主要毒力因子的产生，包括pyocyanin（72.66%）、总蛋白酶（74.66%）、海藻酸盐（66.95%）、胞外多糖（79.14%）、pyoverdine（79.92%）和pyochelin（68.28%）。此外，生物膜的形成减少了83.71%，通过基于导管的生物膜检测和显微镜分析进一步验证了这一点。PAL还损害了游泳、群集和抽搐运动，表明它干扰了最初的生物膜形成。基因表达谱证实了主要QS调控因子（lasR、rhlR、pqsR）及相关毒力基因的下调。在硅研究中，PAL与LasR、RhlR和PqsR的稳定结合得到了良好的对接分数和分子动力学模拟的证实。药代动力学预测显示良好的口服生物利用度和肠道吸收，无明显毒性。利用秀丽隐杆线虫和大黄蜂的感染模型进行的体内验证表明，宿主存活率提高，病原体负担减轻。这些发现表明PAL是一种潜在的QS和生物膜抑制剂，在铜绿假单胞菌感染的治疗管理中具有巨大的潜力。

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引用次数: 0

Enrichment and cultivation of thermophilic Bathyarchaeia from oil reservoirs via systematic cultivation profiling 油藏中嗜热深海菌的富集与培养

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-12-23 DOI: 10.1016/j.ibiod.2025.106270

Qing-Ping He , Yi-Fan Liu , Bo-Wen Wang , Lei Zhou , Shi-Zhong Yang , Ji-Dong Gu , Bo-Zhong Mu

Bathyarchaeia, among the most ubiquitous and abundant microorganisms in nature, play pivotal roles in global carbon cycling. While efforts on their enrichment, classification, genomic, and transcriptomic profiles have been extensively made, pure culture or co-culture with highly enriched Bathyarchaeia remains challenging, particularly in subsurface ecosystems like oil reservoirs. Here, we present a systematic workflow that integrated the Hungate roll-tube technique with 16S rRNA gene amplicon sequencing of total biomass to evaluate cultivation outcomes and identify optimal conditions for Bathyarchaeia by analyzing substrates and temperatures. Under cultivation conditions with various methoxylated aromatic substrates (lignin, syringaldehyde, vanillin, and 3,4,5-trimethoxybenzoate) and three temperatures, this approach led to the successful acquisition of 45 co-cultures containing Bathyarchaeia. Phylogenetic analysis revealed that all recovered Bathyarchaeia operational taxonomic units belonged to subgroup Bathy-6. Co-occurrence network analysis and cultivation results suggested mutualistic interactions between Bathy-6, specific bacterial partners (genus Coprothermobacter, genus Desulfocurvibacter, genus Thermotoga, genus Biomaibacter and genus Thermodesulfovibrio), and methanogens (genus Methanothermobacter, genus Methanothrix and genus Ca. Methanomethylicus) during the degradation of methoxylated aromatics. The optimal cultivation condition for Bathy-6 was determined to be 46 °C with syringaldehyde as the substrate. Furthermore, a novel and efficient DNA extraction protocol from gellan gum-embedded biomass was developed using sodium citrate acidification. Our study provides a effective cultivation strategy for accessing the cryptic Bathyarchaeia and yields a valuable repository of co-cultures. These findings significantly advance our comprehension of the metabolic capabilities and mutualistic lifestyle of Bathyarchaeia in the anaerobic degradation of aromatic compounds within oil reservoir ecosystems.

深海古菌是自然界中最普遍、数量最多的微生物之一，在全球碳循环中起着关键作用。尽管人们对深海古菌的富集、分类、基因组和转录组谱进行了广泛的研究，但纯培养或与高度富集的深海古菌共培养仍然具有挑战性，特别是在油藏等地下生态系统中。在这里，我们提出了一个系统的工作流程，将Hungate滚管技术与总生物量的16S rRNA基因扩增子测序相结合，通过分析底物和温度来评估培养结果，并确定最佳的深海古菌培养条件。在不同的甲氧基化芳香底物（木质素、丁香醛、香兰素和3,4,5-三甲氧基苯甲酸酯）和三种温度的培养条件下，该方法成功地获得了45个含有深海古菌的共培养物。系统发育分析表明，所有恢复的深海archaeia操作分类单位均属于Bathy-6亚群。共生网络分析和培养结果表明，在甲氧基化芳烃降解过程中，Bathy-6与特定的细菌伙伴（Coprothermobacter属、Desulfocurvibacter属、Thermotoga属、Biomaibacter属和Thermodesulfovibrio属）和产甲烷菌（Methanothermobacter属、Methanothrix属和Ca. Methanomethylicus属）相互作用。确定了Bathy-6的最佳培养条件为46℃，丁香醛为底物。此外，开发了一种新的、高效的利用柠檬酸钠酸化从结冷胶包埋的生物质中提取DNA的方法。我们的研究提供了一种有效的培养策略来获取隐蔽的深海古菌，并产生了一个有价值的共培养库。这些发现极大地促进了我们对深海古细菌在油藏生态系统中厌氧降解芳香族化合物的代谢能力和共生生活方式的理解。

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引用次数: 0

Polycyclic aromatic hydrocarbons removal: emerging challenges and innovative solutions 多环芳烃去除：新出现的挑战和创新的解决方案

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-12-22 DOI: 10.1016/j.ibiod.2025.106271

Ahmet Yazıcıoğlu, Sławomir Ciesielski

Polycyclic aromatic hydrocarbons (PAHs) are still important pollutants in environmental health due to intensive anthropogenic activities and natural disasters. Since PAHs are found in mixed forms with other pollutants in real environment, the search for effective and sustainable PAHs removal methods has become a hot issue in this field. The use of biosurfactants in PAHs biodegradation plays a critical role by decreasing surface tension and increasing bioavailability. However, the exact molecular mechanisms and functional relationships between biosurfactants and PAHs are still poorly understood. This review addresses this significant knowledge gap and systematically examines the current strategies to enhance bioprocess efficiency. We propose an innovative synergy between mechanistic biosurfactant research and predictive machine learning approaches. Particularly, we emphasize the application of large language models (LLMs) in clarifying complicated biosurfactant-PAHs interactions. Through the integration of experimental data with LLM-based interaction modeling, we present a forward-looking perspective on how these technologies can accelerate rational biosurfactant design and optimize remediation strategies. We also bring to the environmental engineering community's attention the essential need for curated datasets, benchmarks, and custom models for the successful integration of this technology into bioremediation applications.

由于人类活动和自然灾害的频繁，多环芳烃（PAHs）仍然是影响环境健康的重要污染物。由于多环芳烃在现实环境中与其他污染物以混合形式存在，寻找有效、可持续的多环芳烃去除方法已成为该领域的热点问题。生物表面活性剂在多环芳烃生物降解中起着降低表面张力和提高生物利用度的关键作用。然而，生物表面活性剂与多环芳烃之间的确切分子机制和功能关系仍然知之甚少。这篇综述解决了这一重要的知识差距，并系统地检查了提高生物过程效率的当前策略。我们提出了机械生物表面活性剂研究和预测机器学习方法之间的创新协同作用。特别地，我们强调大语言模型（LLMs）在阐明复杂的生物表面活性剂-多环芳烃相互作用中的应用。通过将实验数据与基于llm的相互作用建模相结合，我们提出了这些技术如何加速合理的生物表面活性剂设计和优化修复策略的前瞻性观点。我们也让环境工程界注意到，为了成功地将这项技术整合到生物修复应用中，对精心策划的数据集、基准和定制模型的基本需求。

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引用次数: 0

Wood deterioration assessment and microbial community analysis of foundation piles experimentally installed for 8 and 10 years 试验安装8年和10年桩基木材劣化评价及微生物群落分析

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-12-17 DOI: 10.1016/j.ibiod.2025.106269

Sitompul Afrida , Ikuo Momohara , Yuka Miyoshi , Takumi Murata , Atsunori Numata , Sakae Horisawa

Buried timber piles play a critical role in ground improvement, yet their long-term deterioration under waterlogged and anaerobic conditions remains insufficiently understood. This study investigated microbial communities and wood degradation in Japanese cedar (Cryptomeria japonica) and Japanese larch (Larix kaempferi) logs used for ground improvement, a geotechnical method that stabilizes soft or liquefiable soils. The logs were buried for 8–10 years at two sites with differing soil compositions and groundwater conditions. Bacterial communities were analyzed using next-generation sequencing (NGS). In cedar, bacterial infiltration extended from the outermost sapwood to the inner sapwood and into the heartwood, likely due to its lower density and higher water permeability. In contrast, bacterial colonization in larch was confined primarily to the outermost sapwood, likely reflecting its higher density and lower permeability. Microscopic analysis revealed erosion of parenchyma cell walls in cedar and slight deterioration of tracheid cell walls in larch, but no apparent reduction in wood density was observed. Dominant bacterial taxa included the anaerobic classes Clostridia and Limnochordia in cedar, and Clostridia and Negativicutes in larch. Facultative anaerobes dominated the surrounding soil, particularly members of the order Chitinophagales. These findings suggest that initial bacterial colonization of the logs was influenced by the soil microbiota and subsequently shaped by their internal environmental conditions. Although fungal communities were identified by ITS amplicon analysis and microscopic observation of cell walls, no apparent signs of fungal degradation were observed. Overall, the results underscore the importance of wood density and permeability in microbial resistance, with larch exhibiting greater durability under anaerobic soil conditions. This study contributes to a deeper understanding of the long-term stability of buried wood in geotechnical applications.

埋地木桩在地基改善中起着至关重要的作用，但其在浸水和厌氧条件下的长期劣化仍未得到充分的了解。本研究调查了日本雪松（Cryptomeria japonica）和日本落叶松（Larix kaempferi）原木的微生物群落和木材退化情况。日本落叶松是一种用于稳定软质或可液化土壤的岩土工程方法。这些原木在两个不同土壤成分和地下水条件的地点埋藏了8-10年。利用下一代测序（NGS）分析细菌群落。在雪松中，细菌的渗透从最外层边材延伸到内部边材，并进入心材，这可能是由于其密度较低，透水性较高。相比之下，落叶松的细菌定植主要局限于最外层的边材，这可能反映了其较高的密度和较低的渗透率。显微分析显示雪松薄壁细胞壁被侵蚀，落叶松管胞细胞壁有轻微退化，但木材密度未见明显下降。优势细菌类群为杉木中的厌氧梭状芽孢杆菌和Limnochordia，落叶松中的梭状芽孢杆菌和阴性芽孢杆菌。兼性厌氧菌在周围土壤中占主导地位，尤其是食几丁菌目的成员。这些发现表明，原木最初的细菌定植受到土壤微生物群的影响，随后受到其内部环境条件的影响。虽然通过ITS扩增子分析和细胞壁的显微镜观察确定了真菌群落，但没有观察到真菌降解的明显迹象。总体而言，结果强调了木材密度和渗透性对微生物抗性的重要性，落叶松在厌氧土壤条件下表现出更强的耐久性。本研究有助于更深入地了解埋地木材在岩土工程应用中的长期稳定性。

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引用次数: 0

Anaerobic mono and co-digestion of cosmetic sewage sludge and organic fraction of municipal solid waste: Methane yield and kinetic modeling 化妆品污水污泥和城市固体废物有机组分的厌氧单一和共消化：甲烷产量和动力学模型

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-12-11 DOI: 10.1016/j.ibiod.2025.106268

Carlos Esteven Pulgarín-Muñoz , Julio César Saldarriaga-Molina , José Francisco Molina-Pérez , Daniela Gonzalez-Pantoja , Lina María Goez-Gaviria

The increasing production of sewage sludge from the cosmetic industry, driven by the sector's continuous growth, has created a pressing need for sustainable treatment and valorization alternatives. These sludges are often complex and challenging to treat due to their physicochemical composition and potential environmental risks. This study evaluates the methane production potential of anaerobic mono-digestion (AMoD) and anaerobic co-digestion (ACoD) of cosmetic industry sewage sludge (CSS) with the organic fraction of municipal solid waste (OFMSW). Four experimental configurations were established with CSS:OFMSW substrate ratios of 100/0, 70/30, 50/50, and 30/70. The results show that increasing the proportion of OFMSW significantly enhances methane production, with the highest yield observed at the 30:70 ratio. The synergistic effect of ACoD was assessed using the Co-Digestion Performance Index (CPI), which indicated a synergistic interaction when the OFMSW proportion exceeded 50 %. Six kinetic models were applied, among which the Modified Gompertz model best described AMoD and ACoD at 70:30 and 50:50 ratios, while the Cone model more accurately represented the system at the 30:70 ratio. This study demonstrates the feasibility of treating CSS through anaerobic digestion and highlights that co-digestion with OFMSW can optimize biogas production, promote sustainable waste management, and enable the valorization of both industrial and municipal organic residues.

在化妆品行业持续增长的推动下，该行业污水污泥的产量不断增加，迫切需要可持续处理和增值替代方案。由于其物理化学成分和潜在的环境风险，这些污泥通常是复杂和具有挑战性的处理。本研究评价了化妆品工业污水污泥（CSS）与城市生活垃圾（OFMSW）有机组分厌氧单消化（AMoD）和厌氧共消化（ACoD）产甲烷的潜力。采用CSS建立了四种实验配置：OFMSW基质比分别为100/0、70/30、50/50和30/70。结果表明：增加OFMSW的添加比例可显著提高甲烷产量，在添加比例为30:70时甲烷产量最高。采用共消化性能指数（CPI）评价ACoD的协同效应，表明当OFMSW的比例超过50%时，ACoD的协同作用存在。采用了6种动力学模型，其中修正的Gompertz模型最能描述70:30和50:50比例下的AMoD和ACoD，而Cone模型更能准确地描述30:70比例下的系统。本研究证明了通过厌氧消化处理CSS的可行性，并强调了与OFMSW共消化可以优化沼气生产，促进废物可持续管理，并实现工业和城市有机残留物的增值。

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引用次数: 0

Biodeterioration of a polyurethane topcoat by Aspergillus niger 黑曲霉对聚氨酯面漆的生物降解

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-12-10 DOI: 10.1016/j.ibiod.2025.106257

Clotilde Maestri , Alexis Duthoit , Ronan L. Hébert , Laure Bredif , Patrick Di Martino

This study investigates the biodeterioration potential of the polyurethane waterproofing coating PU 636 by Aspergillus niger ERAN01 under in vitro conditions. Two protocols, adapted from the ISO 846 standard, were employed using agar media with different nutrient contents. After 9 weeks of incubation at 27 °C and 80 % relative humidity, macroscopic, microscopic, mechanical, and spectroscopic analyses were conducted to assess biodeterioration. Fungal colonization was more extensive in the presence of glucose, which correlated with visible colour changes (notably yellowing), a statistically significant increase in surface roughness, the formation of surface cavities observed via scanning electron microscopy, and chemical modifications detected through infrared spectroscopy. Signs of hydrolysis of ester and urethane bonds, as well as oxidation of ether linkages were observed. Despite these changes, tensile strength measurements showed no significant degradation of the material's mechanical integrity. The results suggest that A. niger produces hydrolases and metabolites capable of inducing greater physical and chemical deterioration of polyurethane coating in the presence of glucose. This study emphasizes the importance of environmental conditions, particularly nutrient availability, in influencing fungal biodeterioration of polymeric materials and supports the need for adapted methodologies when evaluating the biodeterioration of polyurethane waterproofing coatings.

研究了黑曲霉ERAN01在体外条件下对聚氨酯防水涂料PU 636的生物降解潜力。采用不同营养成分的琼脂培养基，采用ISO 846标准的两种方案。在27°C和80%相对湿度条件下培养9周后，进行宏观、微观、力学和光谱分析，以评估生物变质情况。在葡萄糖存在的情况下，真菌的定植更广泛，这与可见的颜色变化（特别是变黄）、表面粗糙度的统计显着增加、通过扫描电子显微镜观察到的表面空腔的形成以及通过红外光谱检测到的化学修饰相关。观察到酯和聚氨酯键水解以及醚键氧化的迹象。尽管有这些变化，拉伸强度测量显示材料的机械完整性没有明显的退化。结果表明，在葡萄糖的存在下，黑曲霉产生的水解酶和代谢物能够引起聚氨酯涂层更大的物理和化学劣化。这项研究强调了环境条件的重要性，特别是营养物质的可用性，在影响真菌对聚合物材料的生物降解方面，并支持在评估聚氨酯防水涂料的生物降解时需要适应的方法。

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引用次数: 0