International Biodeterioration & Biodegradation最新文献_第6页

Interactions between deep microbial biosphere and geo-sequestrated CO2: A review 深层微生物生物圈与地质封存的二氧化碳之间的相互作用：综述

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-26 DOI: 10.1016/j.ibiod.2024.105958

Xuejiao Zhu , Bukang Wang , Yaya Yuan , Jianqiang Lei , Chunxiang Qian

CO₂ capture and storage (CCS) is a promising approach for significantly reducing CO₂ emission from industrial ends and mitigating global warming in the coming decades. Given that most of the subsurface formations are biological active environments, the deep microbial biosphere plays an important role in governing the fate and sequestration efficiency of injected CO₂. While substantial researches have explored the initial states and microbial shifts following CO₂ injection, there remains a notable gap in studies that systematically examine the interactions between deep microbial biospheres and geologically injected CO₂. Such understanding is essential for elucidating mechanisms and extents of geochemical CO₂ evolution under the subsurface microbial mediation. This paper comprehensively examines recent findings about impacts of CO₂ injection on deep microbial biosphere, and discusses microbial responses that are pivotal in determining the fate of the injected CO₂. Initially, the review addresses the influence of elevated CO₂ on geological chemistry, including pH, ionic strength, redox state and free energy distribution. Subsequently, it delves into the adverse effects of CO₂ on microbial growth, cell integrity, endospore generation, metabolism and community dynamics. Lastly, the review emphasizes key microbial activities that potentially facilitate in situ CO₂ immobilization, including CO₂ mineralization, methanogenesis and conversion to other valued chemicals. The objective of this review is to elucidate how elevated CO₂ levels affect geological microbiology, and how microbial responses impact the fate and speciation of CO₂ in storage sites, and to provide insights for optimizing future CO₂ storage strategies in geological formations.

二氧化碳捕集与封存（CCS）是未来几十年大幅减少工业排放的二氧化碳和减缓全球变暖的一种可行方法。鉴于大多数地下地层都是生物活跃的环境，深层微生物生物圈在控制注入的二氧化碳的归宿和封存效率方面发挥着重要作用。虽然已有大量研究探讨了二氧化碳注入后的初始状态和微生物变化，但系统研究深层微生物生物圈与地质注入的二氧化碳之间相互作用的研究仍是一个明显的空白。这种认识对于阐明地下微生物介导下二氧化碳地球化学演化的机制和程度至关重要。本文全面研究了有关二氧化碳注入对深层微生物生物圈影响的最新发现，并讨论了对决定注入的二氧化碳命运至关重要的微生物反应。文章首先探讨了高浓度二氧化碳对地质化学的影响，包括 pH 值、离子强度、氧化还原状态和自由能分布。随后，文章深入探讨了二氧化碳对微生物生长、细胞完整性、内孢子生成、新陈代谢和群落动态的不利影响。最后，综述强调了有可能促进二氧化碳就地固定化的关键微生物活动，包括二氧化碳矿化、甲烷生成和转化为其他有价值的化学物质。本综述旨在阐明升高的二氧化碳水平如何影响地质微生物学，以及微生物的反应如何影响二氧化碳在封存地点的归宿和物种，并为优化地质构造中未来的二氧化碳封存战略提供见解。

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引用次数: 0

Effects of reclamation on the distribution and diversity of comammox along the coastal wetlands of China 填海对中国滨海湿地复合微生物分布和多样性的影响

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-23 DOI: 10.1016/j.ibiod.2024.105956

Dongyao Sun , Yinghui Jiang , Hengchen Wei , Jun Li , Dengzhou Gao , Xianbiao Lin , Cheng Chen , Guoyu Yin , Yifei Zhang , Lijun Hou , Min Liu , Ping Han

Reclamation of estuarine wetlands for paddy fields (PF) and aquaculture ponds (AP) used to be a common practice in China, which has changed land types and significantly affected microbial nitrogen transformations. However, its impacts on nitrification process especially complete ammonia oxidizers (comammox) remain poorly understood. Our study investigated the distribution and diversity of comammox across the major estuarine wetland systems along the coast of China by comparing different land types, including paddy fields (PF), aquaculture ponds (AP), and wetlands. The results showed that reclamation for PF and AP (1.94 × 10⁶ copies g⁻¹ soil) significantly reduced the gene abundance of comammox compared with wetlands (3.19 × 10⁶ copies g⁻¹ soil), with higher ammonia-oxidizing archaea (AOA) and bacteria (AOB) abundances and the rates of nitrification in PF than in AP. Phylogenetic analysis revealed different community structures in the two reclamation types. The distribution pattern of comammox genes in PF was similar to that in estuarine wetlands, with clade A1 and clade A2.1 being the dominant branches. Water content, iron, sulfide, and salinity were identified as the key factors affecting the distribution of comammox. This study highlights the impact of reclamation on comammox in estuarine wetlands and expands our understanding of nitrogen cycling processes in estuarine systems.

在中国，将河口湿地围垦为水田（PF）和水产养殖池塘（AP）曾经是一种常见的做法，这种做法改变了土地类型，并对微生物氮转化产生了重大影响。然而，人们对其对硝化过程的影响，尤其是对完全氨氧化剂（comammox）的影响仍然知之甚少。我们的研究通过比较不同的土地类型，包括水田（PF）、水产养殖池塘（AP）和湿地，调查了comammox在中国沿海主要河口湿地系统中的分布和多样性。结果表明，与湿地（3.19×106 拷贝 g-1 土壤）相比，水稻田和水产养殖塘（1.94×106 拷贝 g-1 土壤）的开垦明显降低了复合氧化酶的基因丰度，水稻田的氨氧化古细菌（AOA）和细菌（AOB）丰度以及硝化率均高于水产养殖塘。系统发育分析表明，两种填海类型的群落结构不同。PF 中 comammox 基因的分布模式与河口湿地相似，A1 支系和 A2.1 支系为主要分支。含水量、铁、硫化物和盐度被认为是影响 comammox 分布的关键因素。这项研究强调了填海对河口湿地中 comammox 的影响，并拓展了我们对河口系统中氮循环过程的认识。

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引用次数: 0

Feasibility of successive hydrogen and methane production: Effects of temperature and organic loads on energy potential and microbial dynamics 连续生产氢气和甲烷的可行性：温度和有机负荷对能量潜力和微生物动态的影响

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-20 DOI: 10.1016/j.ibiod.2024.105955

Kauanna Uyara Devens , Alexandre Rodrigues Ribeiro , Franciele Pereira Camargo , Isabel Kimiko Sakamoto , Maria Bernadete Amâncio Varesche , Edson Luiz Silva

This study aims to assess the co-digestion of Cassava Wastewater (CW) and glycerol in a two-stage process using fluidized bed reactors (AFBR), verifying the effect of organic loading rate (OLR) and temperature (mesophilic [SMR] and thermophilic [STR]) in sequential reactors on CH₄ production. The OLR ranged from 1.2 to 15 g COD.L⁻¹.d⁻¹ and the hydraulic retention time (HRT) was set at 20 h. The mesophilic sequential reactor (MSR) (average temperature of 30 °C) showed greater tolerance to high OLR and its best MPR was 101.12 mL of CH₄.d⁻¹.L⁻¹ h⁻¹, obtained at a OLR of 15 g COD.L⁻¹.d⁻¹). The maximum yield was 341.10 mL of CH₄.g⁻¹COD_cons, found at the OLR of 1.2 g COD.L⁻¹.d⁻¹. The sequential thermophilic reactor (STR) showed the maximum yield and MPR of 333.03 mL of CH₄.g⁻¹COD_cons (1.2 g COD.L⁻¹.d⁻¹) and 58.84 mL of CH₄.d^{− 1}.L⁻¹ h⁻¹ (12 g COD.L⁻¹.d⁻¹), respectively. Through the massive sequencing analysis of the 16S rRNA gene, it was possible to observe a greater diversity of microorganisms in the TSR than in the MSR. A predominance of acetoclastic microorganisms was observed, with the genera Methanobacterium, Methanosarcina and Methanobrevibacter being the most abundant in both reactors. The two-stage system composed of mesophilic acidogenic reactor + MSR was more suitable for the co-digestion of CW and glycerol than the acidogenic reactor + TSR. These results support the notion of standard operating conditions at the industrial plant, where the cassava processing process is carried out at room temperature (25–30 °C).

本研究旨在评估木薯废水（CW）和甘油在流化床反应器（AFBR）两阶段工艺中的协同消化情况，验证顺序反应器中有机负荷率（OLR）和温度（嗜中[SMR]和嗜热[STR]）对 CH4 产量的影响。嗜中性顺序反应器（MSR）（平均温度为 30 °C）对高 OLR 的耐受性更强，其最佳 MPR 为 101.12 mL CH4.d-1.L-1 h-1（OLR 为 15 g COD.L-1.d-1）。当 OLR 为 1.2 g COD.L-1.d-1 时，最高产率为 341.10 mL CH4.g-1CODcons。序贯嗜热反应器（STR）的最大产率和最大增产率分别为 333.03 mL CH4.g-1CODcons (1.2 g COD.L-1.d-1) 和 58.84 mL CH4.d- 1.L-1 h-1 (12 g COD.L-1.d-1) 。通过对 16S rRNA 基因的大量测序分析，可以观察到 TSR 中微生物的多样性高于 MSR。在两个反应器中，甲烷杆菌属、甲烷弧菌属和甲烷杆菌属的数量都最多。由中嗜酸性产酸反应器 + MSR 组成的两级系统比产酸反应器 + TSR 更适合用于化武和甘油的共消化。这些结果支持了工业工厂标准操作条件的概念，即木薯加工过程是在室温（25-30 °C）下进行的。

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引用次数: 0

Identification of the key factors influencing biodeterioration of open-air cultural heritage in the temperate climate zone of China 确定影响中国温带气候区露天文物生物退化的关键因素

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-16 DOI: 10.1016/j.ibiod.2024.105954

Chong Wang , Li Wang , Chongbin Bai , Mingpeng Wang , Tao Ma , Honglin Ma , Guangming Zhang , Weiwei Wang , Zisheng Guo , Yanmei Sun , Shiwei Wang

Biodeterioration is known to cause irreversible damage to open-air cultural heritages. China has numerous open-air cultural heritages constructed of stone and brick, but very little information is available about the factors involved in their biodeterioration. In this study, different kinds of open-air cultural heritages (steles, towers, temples, Tang-ling carvings, and stone bridges) from 14 different locations in temperate climate zone of China were collected and investigated. Microbiological characteristics of these cultural heritages revealed that both cyanobacteria and lichens were the mainly dominant groups on these open-air stone and brick cultural heritages with visible deterioration. Cyanobacteria were closely associated with the severely deteriorated Tang-ling carvings wile lichens were most commonly found on temples and bridges. The main cyanobacteria were Oscillatoria, Pleurocapsa, and Lyngbya, and the dominant lichens were Xanthoria Th. Fr., Lepraria. Ach. and Camellia on the surface of cultural heritage. In addition, the extent of deterioration associated with cyanobacteria exhibited an increasing trend with higher annual mean sunshine intensity, calcite content, and cultural heritage age though not statistically significant. Redundancy analysis indicated that sunshine intensity and the age of the archeological structures were the key factors that significantly affected the biodeterioration of cultural heritages. This study provides more understanding of the microbial community associated with deterioration of open-air cultural heritages situated at the temperate climate zone of China.

众所周知，生物退化会对露天文物造成不可逆转的破坏。中国有大量的石质和砖质露天文物，但有关其生物劣化因素的信息却很少。本研究收集并调查了中国温带气候区 14 个不同地点的各种露天文物（石碑、塔、寺庙、唐陵雕刻和石桥）。这些文物的微生物学特征表明，蓝藻和地衣是这些有明显退化的露天石砖文物上的主要优势菌群。蓝藻与严重破损的唐陵石刻密切相关，而地衣则多见于寺庙和桥梁。主要的蓝藻有 Oscillatoria、Pleurocapsa 和 Lyngbya，主要的地衣有 Xanthoria Th. Fr.Fr.、Lepraria.Ach. 和山茶花。此外，随着年平均日照强度、方解石含量和文物年代的增加，与蓝藻相关的劣化程度呈上升趋势，但无统计学意义。冗余分析表明，日照强度和考古结构的年代是显著影响文物生物劣化的关键因素。本研究有助于进一步了解中国温带气候区露天文物退化的相关微生物群落。

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引用次数: 0

The impacts of flagellin on larval metamorphosis of mussel Mytilus coruscus varied with protein structure 鞭毛蛋白对贻贝幼体变态的影响因蛋白质结构而异

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-15 DOI: 10.1016/j.ibiod.2024.105952

Tiantian Liu , Yu Tao , Yushan Cai , Lihua Peng , Jin-Long Yang , Xiao Liang

Flagellin produced by Pseudoalteromonas marina has been shown to induce larval metamorphosis in mussels. The flagellin protein FliC was composed of four subunits encoded by the genes fliC-02330, fliC-02345, fliC-02346, and fliC-02347. In this study, we investigated the effects of these four FliC proteins on biofilm formation and larval metamorphosis. The inducing activity of the recombinant proteins FliC-02345, FliC-02346, and FliC-02347 was similar and significantly higher (p < 0.05) than that of FliC-02330. When compared to the wild-type strain, biofilm formation was significantly enhanced in the four fliC mutants, whereas the inducing activity of four ΔfliC biofilms decreased. Among these mutants, ΔfliC-02330 exhibited the highest thickness, density, and c-di-GMP levels among its respective biofilms. The inducing activity of the ΔfliC-02330 biofilm increased by 30–40% compared to the inducing activities of other mutant strains. Furthermore, structural analysis revealed differences in protein structure and phosphorylation sites between FliC-02330 and its counterparts (FliC-02345, FliC-02346, and FliC-02347), suggesting that variations in protein structure contribute to differing impacts on both biofilm formation and larval metamorphosis. These findings provide new insights into the interaction between bacterial flagellin protein and the larval metamorphosis of marine invertebrates.

已证明假交替单胞菌（Pseudoalteromonas marina）产生的鞭毛蛋白可诱导贻贝幼虫变态。鞭毛蛋白 FliC 由四个亚基组成，分别由 fliC-02330、fliC-02345、fliC-02346 和 fliC-02347 基因编码。在本研究中，我们研究了这四种 FliC 蛋白对生物膜形成和幼虫变态的影响。重组蛋白FliC-02345、FliC-02346和FliC-02347的诱导活性与FliC-02330相似，且显著高于FliC-02330（p <0.05）。与野生型菌株相比，四个 fliC 突变体的生物膜形成明显增强，而四个 ΔfliC 生物膜的诱导活性降低。在这些突变体中，ΔfliC-02330 的生物膜厚度、密度和 c-di-GMP 水平最高。与其他突变株的诱导活性相比，ΔfliC-02330 生物膜的诱导活性提高了 30-40%。此外，结构分析表明，FliC-02330 与其对应品系（FliC-02345、FliC-02346 和 FliC-02347）的蛋白质结构和磷酸化位点存在差异，这表明蛋白质结构的变化对生物膜的形成和幼虫的变态产生了不同的影响。这些发现为了解细菌鞭毛蛋白与海洋无脊椎动物幼虫变态之间的相互作用提供了新的视角。

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引用次数: 0

Comprehending microplastic pollution in diverse environment: Assessing fate, impacts, and remediation approaches 了解不同环境中的微塑料污染：评估归宿、影响和补救方法

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-12 DOI: 10.1016/j.ibiod.2024.105953

Priyanka Yadav , Vishal Mishra

Microplastics (MPs) pose pervasive environmental threats that potentially harm ecosystems and public health. Till date, most of the researches have predominantly concentrated on freshwater and marine ecosystems. This review attempts to provide current knowledge of microplastic pollution, its source, and its fate in all environmental compartments for instance soil, water, and atmosphere. Also briefly summarizes different MP's exposure routes and their negative consequences on public health. Various conventional methods have been used to remove MPs but these are inefficient owing to several demerits. Microbial approach is regarded as a safer option for removing MPs due to its unique metabolic mechanism. More than 400 microorganisms have been discovered as capable of MPs degradation. Fungi have appeared as the most proficient degraders of plastics among the microbes because they release unique enzymes and can thrive on hazardous components with low resources. Numerous fungal species that can break down various plastic kinds have been found in recent studies; nevertheless, there are still many unanswered questions regarding the mechanisms underlying biodegradation. The molecular mechanisms behind MP degradation via various enzymes have received little attention. Also, white-rot fungi have received the least attention among the fungi examined for biodegradation despite having a unique extracellular enzymatic system. Thus, the current study summarizes the ability of fungi that degrade various polymers and the function of multiple enzymes in the degradation of MPs. Also, the role of different extracellular and intracellular enzymes in biological degradation of MPs is discussed. Prospects for the future include tracking long-term trends in microplastic contamination, evaluating the effectiveness of clean-up techniques, and integrating the developed technologies with socioeconomic factors into policy frameworks.

微塑料（MPs）对环境构成了普遍威胁，有可能损害生态系统和公众健康。迄今为止，大多数研究主要集中在淡水和海洋生态系统。本综述试图提供有关微塑料污染、其来源及其在土壤、水和大气等所有环境区划中的归宿的最新知识。此外，还简要概述了不同微塑料的接触途径及其对公众健康的负面影响。人们使用了各种传统方法来清除 MPs，但这些方法由于存在一些缺点而效率低下。微生物方法因其独特的新陈代谢机制而被认为是去除 MPs 的更安全选择。目前已发现 400 多种能够降解 MPs 的微生物。真菌是微生物中最擅长降解塑料的，因为它们能释放独特的酶，并能以较少的资源在有害成分上生长。最近的研究发现了许多能分解各种塑料的真菌种类；然而，关于生物降解的基本机制仍有许多未解之谜。通过各种酶降解 MP 的分子机制很少受到关注。此外，尽管白腐真菌具有独特的胞外酶系统，但在所研究的生物降解真菌中，白腐真菌受到的关注最少。因此，本研究总结了真菌降解各种聚合物的能力以及多种酶在降解 MPs 过程中的作用。此外，还讨论了不同的细胞外酶和细胞内酶在生物降解 MPs 中的作用。未来展望包括跟踪微塑料污染的长期趋势、评估清理技术的有效性，以及将开发的技术与社会经济因素纳入政策框架。

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引用次数: 0

Comparative studies on the effectiveness of proteases and enzymes from dietary supplements in the degradation of polylactide (PLA) 蛋白酶和膳食补充剂中的酶在降解聚乳酸（PLA）中的效果比较研究

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-10 DOI: 10.1016/j.ibiod.2024.105950

Katarzyna Janczak , Alicja Mazuryk , Daria Lisewska , Natalia Puszczykowska , Kacper Fiedurek

Polylactide (PLA) has become one of the most widely used biodegradable plastics in recent years. The research focuses on solving the problem of its low-rate biodegradability under mesophilic conditions using cost-effective proteases of alternative origin. The aim of the study was to verify whether enzymes contained in food dietary supplements with the suggested high activity of protease enzymes could potentially be used for the degradation of PLA. The studies compare the activity of enzymes from dietary supplements with hydrolytic enzymes with scientifically proven effectiveness in degrading PLA. Products containing nattokinase, bromelain, papain and an enzyme mixture were tested. Their effectiveness against proteinase K, subtilisin and lipase was compared. From this group, proteinase K caused the greatest PLA mass loss and increase in O/C ratio after 28 days of incubation. The results indicated that nattokinase and papain caused a mass loss of PLA films similar to that of proteinase K, as well as a decrease in the carbonyl index value by more than 20%. Additionally, studies have shown that dietary supplements have a beneficial effect on the compost microflora. Causing an approximately 20-fold increase in the number of microorganisms within 48 h. In accordance with the assumptions, enzymatic products and their concentrations were selected that effectively accelerate PLA degradation in mesophilic conditions at a level comparable to or better than proteinase K, but have a much better effect on the substrate microflora, which makes them an interesting object for the development of products for accelerating the degradation of polymeric materials. In order to confirm the possibility of practical use of the results, an analysis of the phytotoxicity of the enzymes was carried out, which confirmed the possibility of using the enzymes in natural conditions.

聚乳酸（PLA）是近年来应用最广泛的生物降解塑料之一。这项研究的重点是利用具有成本效益的替代来源蛋白酶，解决聚乳酸在中温条件下生物降解率低的问题。研究的目的是验证食品膳食补充剂中所含的酶是否具有蛋白酶的高活性，是否有可能用于降解聚乳酸。研究将膳食补充剂中的酶的活性与经科学证明可有效降解聚乳酸的水解酶进行了比较。对含有纳豆激酶、菠萝蛋白酶、木瓜蛋白酶和酶混合物的产品进行了测试。比较了它们对蛋白酶 K、枯草酶和脂肪酶的功效。在这组产品中，蛋白酶 K 在培养 28 天后造成的聚乳酸质量损失和 O/C 比率增加最大。结果表明，纳豆激酶和木瓜蛋白酶造成的聚乳酸薄膜质量损失与蛋白酶 K 相似，羰基指数值下降了 20% 以上。此外，研究还表明，膳食补充剂对堆肥微生物区系有好处。根据上述假设，我们选择了酶制剂产品及其浓度，它们能有效加速聚乳酸在中温条件下的降解，其降解效果与蛋白酶 K 相当或更好，但对底物微生物群的影响要大得多，这使它们成为开发加速高分子材料降解产品的有趣对象。为了证实这些结果的实际应用可能性，对酶的植物毒性进行了分析，证实了在自然条件下使用酶的可能性。

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引用次数: 0

Bioremediation approaches for chromium detoxification and transformation: Advanced strategies and future Perspectives 铬解毒和转化的生物修复方法：先进战略与未来展望

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-09 DOI: 10.1016/j.ibiod.2024.105951

Arukula Deepa , Anthati Mastan , Viswanath Buddolla , Yedluri Anil Kumar , Buddolla Anantha Lakshmi , Young-Joon Kim

Hexavalent chromium [Cr(VI)] contamination poses a significant environmental and public health risk due to its widespread industrial use, toxic nature, and carcinogenic properties. Conventional Cr(VI) remediation methods, including electrochemical processes, ion exchange, reverse osmosis, and adsorption, face challenges such as high energy consumption and incomplete detoxification. This review provides a comprehensive evaluation of cutting-edge bioremediation techniques for Cr(VI) removal, with an emphasis on microbial mechanisms involving bacteria and fungi. Bioremediation approaches, particularly those utilizing Cr(VI)-resistant microorganisms, present promising, eco-friendly, and cost-effective solutions for chromium pollution mitigation. The mechanisms of biosorption, bioaccumulation, and bio-reduction employed by these microorganisms are thoroughly examined. Furthermore, the review addresses the scalability and practical application of these biotechnologies in wastewater treatment facilities. This analysis highlights the critical need for innovative, sustainable chromium bioremediation strategies and outlines future research directions to advance this vital field.

六价铬[Cr(VI)]污染因其广泛的工业用途、毒性和致癌特性，对环境和公共健康构成了重大风险。包括电化学过程、离子交换、反渗透和吸附在内的传统六价铬修复方法面临着高能耗和解毒不彻底等挑战。本综述全面评估了去除六价铬的尖端生物修复技术，重点是涉及细菌和真菌的微生物机制。生物修复方法，特别是那些利用抗六价铬微生物的方法，为减轻铬污染提供了前景广阔、生态友好和经济高效的解决方案。综述深入研究了这些微生物的生物吸附、生物累积和生物还原机制。此外，研究还探讨了这些生物技术在废水处理设施中的可扩展性和实际应用。这项分析强调了对创新型、可持续铬生物修复战略的迫切需要，并概述了推进这一重要领域的未来研究方向。

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引用次数: 0

Co-fermentation of dark septate endophytes and Trichoderma viride can effectively degrade cellulose in different Chinese medicinal herbal residues 暗色隔内生菌和毛霉共同发酵可有效降解不同中药渣中的纤维素

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-05 DOI: 10.1016/j.ibiod.2024.105949

Wanyun Li , Menghui Wang , Xuemin Zhang , Yali Xie , Wenjing Chen , Li Yao , Chao He , Xueli He

Chinese medicinal herbal residues (CMHRs) are currently a widely overlooked renewable biomass resource, and developing controllable microbial fermentation technology for CMHRs will help achieve the resource utilization of CMHRs. In this study, the activity of various cellulases, reducing sugar content and cellulose degradation rate were combined to evaluate the solid state fermentation (SSF) potential of dark septate endophytes (DSE) and Trichoderma viride in separate fermentation and co-fermentation of different CMHRs (single Astragalus mongolicus residue and compound residues of Panax notoginseng and Salvia miltiorrhiza). The results showed that the cellulase activity of some DSE strains was higher than that of T. viride. T. viride fermentation alone could increase the content of substrate reducing sugars, while DSE fermentation could reduce the content of substrate reducing sugars. DSE strains such as Macrophomina pseudophaseoline, Paraboeremia selaginellae, Paraphoma chlamydocopiosa and Paraphoma radicina had the potential to co-ferment with T. viride. Optimization of the fermentation process by inoculation of T. viride after 3 d or 6 d of DSE effectively increased the cellulase activity of co-fermented CMHRs. After optimization, the activities of filter paper cellulase, carboxymethyl cellulase, and β-glucosidase in different fermentation combinations reached 2.51–5.61, 2.86–4.72 and 12.85–32.35 U/g, respectively. Further, the co-fermentation of DSE and T. viride effectively degraded cellulose in different CMHRs with a degradation rate of 54.43%–67.38%. Scanning electron microscope confirmed that the co-fermentation of DSE and T. viride could increase the external surface area and porosity of CMHRs, and destroy the structure of CMHRs. The results of this study both provided a basis for the microbial degradation of single and compound CMHR, which is of great significance for solving the treatment of waste such as Chinese herbal residue and further resource utilization.

中药渣是目前被广泛忽视的可再生生物质资源，开发可控的中药渣微生物发酵技术将有助于实现中药渣的资源化利用。本研究结合各种纤维素酶的活性、还原糖含量和纤维素降解率，评估了暗隔内生菌（DSE）和毛霉菌在不同中草药残体（单一黄芪残体、三七和丹参的复合残体）的单独发酵和联合发酵中的固态发酵（SSF）潜力。结果表明，一些 DSE 菌株的纤维素酶活性高于毒死蜱。单纯的病毒发酵可增加底物还原糖的含量，而 DSE 发酵则可降低底物还原糖的含量。DSE菌株，如假嗜酸性大酵母菌、Paraboeremia selaginellae、Paraphoma chlamydocopiosa和Paraphoma radicina，具有与T. viride共同发酵的潜力。通过在 DSE 3 d 或 6 d 后接种 T. viride 来优化发酵过程，可有效提高共发酵 CMHR 的纤维素酶活性。优化后，不同发酵组合的滤纸纤维素酶、羧甲基纤维素酶和β-葡萄糖苷酶的活性分别达到了2.51-5.61、2.86-4.72和12.85-32.35 U/g 。此外，DSE 和毒死蜱共同发酵可有效降解不同 CMHR 中的纤维素，降解率为 54.43%-67.38%。扫描电子显微镜证实，DSE 和病毒菌共同发酵能增加 CMHR 的外表面积和孔隙率，破坏 CMHR 的结构。该研究结果为微生物降解单一和复合中草药残渣提供了依据，对解决中草药残渣等废弃物的处理和进一步资源化利用具有重要意义。

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引用次数: 0

Basalt rock weathering by Peribacillus simplex from Wudalianchi volcanos in NE China and implications for Fe and Si biogeochemical cycling 中国东北五大连池火山的玄武岩岩石风化及对铁和硅生物地球化学循环的影响

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2024-11-02 DOI: 10.1016/j.ibiod.2024.105948

Shuang Zhang , Gaofei Ying , Tao Liu , Jiani Yang , Enci Zhu , Xindi Sun , Ji-Dong Gu , Lei Yan

Bacteria have an important role in weathering the different rocks, but little is known about the mechanism of microbial weathering in basalt rocks. In the present study, Peribacillus simplex WS-L19 isolated from Wudalianchi volcanos exhibits the highest Fe and Si rock dissolution, which revealed unique weathering ability. The optimal weathering conditions of LB medium diluted at 30.9%, the rotation speed of 127.2 rpm, and temperature at 36.6 °C, resulted in the Fe and Si releases of 1.70 ± 0.09 μg/mL and 20.11 ± 1.13 μg/mL, respectively. Simultaneously, complex ligands such as organic acids (tartaric acid, formic acid, lactic acid and succinic acid), siderophores, and biofilm were also found to be involved in weathering processes by P. simplex WS-L19. Kinetics study of Fe and Si release indicated that the weathering processes of volcanic rocks mediated by P. simplex WS-19 fits a Hyperbl model. Mineralogical analysis showed a decrease in Fe releasing ratio by 44.65% and an increase in Si releasing ratio by 28.45%, showing that Fe is more accessible than Si. P. simplex WS-L19 had a significant weathering effect on biotite, and Si-O bonding vibration might contribute to weathering. The results suggest that indigenous P. simplex WS-L19 plays a role in weathering Wudalianchi volcanos and provides new insights into the Fe and Si biogeochemical cycling of basalt rocks.

细菌在不同岩石的风化过程中发挥着重要作用，但人们对玄武岩微生物风化的机理知之甚少。在本研究中，从五大连池火山中分离出的单纯弧菌 WS-L19 对岩石中铁和硅的溶解度最高，显示了其独特的风化能力。在稀释度为 30.9% 的 LB 培养基、转速为 127.2 rpm、温度为 36.6 °C 的最佳风化条件下，Fe 和 Si 的释放量分别为 1.70 ± 0.09 μg/mL 和 20.11 ± 1.13 μg/mL。与此同时，还发现有机酸（酒石酸、甲酸、乳酸和琥珀酸）、嗜硒体和生物膜等复合配体也参与了单纯栉水母 WS-L19 的风化过程。铁和硅释放动力学研究表明，单胞菌 WS-19 介导的火山岩风化过程符合 Hyperbl 模型。矿物学分析表明，铁的释放率降低了 44.65%，而硅的释放率提高了 28.45%，这表明铁比硅更容易获得。单胞菌 WS-L19 对黑云母有明显的风化作用，Si-O 键振动可能有助于风化。研究结果表明，本地单胞菌 WS-L19 在五大连池火山风化过程中发挥了作用，并为玄武岩中铁和硅的生物地球化学循环提供了新的见解。

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引用次数: 0