Pub Date : 2025-10-09DOI: 10.1016/j.ibiod.2025.106225
Qianwei Li, Hairun Ma, Miao Zhang, Biao Wei, Daoqing Liu
Microplastics (MPs) with high surface area and reactivity readily bind microbial proteins to form the environment's MP–protein corona complexes (MP-PCs). Here, MPs leached from disposable face masks were characterized and incubated with extracellular proteins from Staphylococcus amber (S. amber) to induce MP-PCs formation. SDS-PAGE and microplate assays confirmed the variability of the MP-PCs, while chemical analysis revealed the presence of six detectable heavy metals (Cu, Fe, Mn, Pb, Cr, Zn) and slight pH changes in the leachates. The formation of MP-PCs facilitates the adsorption of heavy metals onto MPs and modulates MP-cell interactions, thereby enhancing the generation of bacterial reactive oxygen species (ROS). Moreover, the ROS produced by bacteria, catalyzed by transition metals adsorbed on the protein corona, contribute to the degradation of MPs through a Fenton-like reaction. These findings underscore the complex ecological risks associated with mask-derived MPs, which not only inhibit microbial growth but also accelerate their environmental transformation.
{"title":"Synergistic effects of protein coronas and heavy metals on ROS generation: Implications for microplastic-microbe interactions","authors":"Qianwei Li, Hairun Ma, Miao Zhang, Biao Wei, Daoqing Liu","doi":"10.1016/j.ibiod.2025.106225","DOIUrl":"10.1016/j.ibiod.2025.106225","url":null,"abstract":"<div><div>Microplastics (MPs) with high surface area and reactivity readily bind microbial proteins to form the environment's MP–protein corona complexes (MP-PCs). Here, MPs leached from disposable face masks were characterized and incubated with extracellular proteins from <em>Staphylococcus amber</em> (<em>S. amber</em>) to induce MP-PCs formation. SDS-PAGE and microplate assays confirmed the variability of the MP-PCs, while chemical analysis revealed the presence of six detectable heavy metals (Cu, Fe, Mn, Pb, Cr, Zn) and slight pH changes in the leachates. The formation of MP-PCs facilitates the adsorption of heavy metals onto MPs and modulates MP-cell interactions, thereby enhancing the generation of bacterial reactive oxygen species (ROS). Moreover, the ROS produced by bacteria, catalyzed by transition metals adsorbed on the protein corona, contribute to the degradation of MPs through a Fenton-like reaction. These findings underscore the complex ecological risks associated with mask-derived MPs, which not only inhibit microbial growth but also accelerate their environmental transformation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"207 ","pages":"Article 106225"},"PeriodicalIF":4.1,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145270884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-08DOI: 10.1016/j.ibiod.2025.106224
Yufei Wu , Ziwei Jiang , Liang Ma , Xiaodong Wu , Qian Lu , Shuying Zang
Biochar amendment to soil is regarded as a promising approach to enhance soil carbon sequestration in agroforestry ecosystems. However, the effects of biochar on the mineralization of soil organic carbon (SOC) in permafrost-affected forest soils and their regulating mechanisms remain unknown. Here, humus layer soil samples from a permafrost-affected Larix gmelinii forest were incubated with biochar additions of CK (no biochar), 2 % (BC2), 4 % (BC4), and 8 % (BC8). Biochar reduced SOC mineralization rates by 4.72 %–7.02 %, with concurrent increases in soil total organic carbon (8.8 %–28.8 %) and dissolved organic carbon (1.5–3.4 times). Soil substrates (NH4+-N, pH, cation exchange capacity, electrical conductivity) and enzyme activities (dehydrogenase, polyphenol oxidase, urease) followed similar trends. Bacterial co-occurrence networks exhibited enhanced complexity and stability (e.g., network size, connectivity, modules, keystone species), with community assembly shifting from deterministic toward stochastic processes. The partial least squares structural equation modeling revealed that biochar might mitigate the SOC mineralization by reducing microbial activity through enhanced bacterial synergistic effects. These results highlight the importance of incorporating wildfire-produced biochar into the projections of permafrost carbon cycle.
{"title":"Enhanced stability of bacterial co-occurrence networks to biochar amendment reduces soil carbon mineralization in permafrost-affected soils","authors":"Yufei Wu , Ziwei Jiang , Liang Ma , Xiaodong Wu , Qian Lu , Shuying Zang","doi":"10.1016/j.ibiod.2025.106224","DOIUrl":"10.1016/j.ibiod.2025.106224","url":null,"abstract":"<div><div>Biochar amendment to soil is regarded as a promising approach to enhance soil carbon sequestration in agroforestry ecosystems. However, the effects of biochar on the mineralization of soil organic carbon (SOC) in permafrost-affected forest soils and their regulating mechanisms remain unknown. Here, humus layer soil samples from a permafrost-affected Larix gmelinii forest were incubated with biochar additions of CK (no biochar), 2 % (BC2), 4 % (BC4), and 8 % (BC8). Biochar reduced SOC mineralization rates by 4.72 %–7.02 %, with concurrent increases in soil total organic carbon (8.8 %–28.8 %) and dissolved organic carbon (1.5–3.4 times). Soil substrates (NH<sub>4</sub><sup>+</sup>-N, pH, cation exchange capacity, electrical conductivity) and enzyme activities (dehydrogenase, polyphenol oxidase, urease) followed similar trends. Bacterial co-occurrence networks exhibited enhanced complexity and stability (e.g., network size, connectivity, modules, keystone species), with community assembly shifting from deterministic toward stochastic processes. The partial least squares structural equation modeling revealed that biochar might mitigate the SOC mineralization by reducing microbial activity through enhanced bacterial synergistic effects. These results highlight the importance of incorporating wildfire-produced biochar into the projections of permafrost carbon cycle.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"207 ","pages":"Article 106224"},"PeriodicalIF":4.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145270882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-06DOI: 10.1016/j.ibiod.2025.106223
Muna Faeq Ali , Nahla Sh. Ajeel , Hasanain Saad Alhares , Sabah J. Mohammed , Mohanad J. M-Ridha
This study investigated phytoremediation of Congo red (CR) and methyl orange (MO) dye-contaminated water samples with Ceratophyllum demersum L. (coontail). Phytotoxicity assessments were also conducted to clarify the effects of dye concentrations on the growth and half-life of the plant. Based on the results, the 100 mg/L dye killed half of the plant (half-life) within 27 days. Increased dye concentration and exposure period also led to elevated toxicity in the plants. The results indicated that the dyes negatively affected plant growth. Conversely, incorporating NPK nutrients contributed to the plants overcoming toxicity and enhanced their growth. Furthermore, the MO dye exhibited fewer negative effects on the plant than the CR dye. Nevertheless, the phytoremediation process applied was successful. A 20 g of the coontail plant (dye solution volume = 5 L, initial pH = 7.0, initial dye level = 25 mg/L) dye removal percentages 100 % and 98 % CR and MO dyes during 15 days of exposure, respectively. FTIR data revealed that the various functional groups on the plant surface enabled coontail to absorb dyes from aqueous solutions, including carboxyl and carbonyl groups. Comparisons of control (without dye) and exposed (25 mg/L of initial dye concentration) coontail FESEM images demonstrated considerably altered morphological properties during phytoremediation of the dyes. These changes indicated effective loading of the dye onto the surfaces of the coontail samples. The uptake of dyes by coontail follows the Michaelis-Menten kinetics, indicating that adsorption occurs through a limited number of active sites on the plant's surface. Consequently, coontail is a notably efficient plant, applicable in eradicating dye-contaminated wastewater.
{"title":"Phytoremediation of Congo red and methyl orange dye-contaminated water with the coontail Ceratophyllum demersum aquatic plant","authors":"Muna Faeq Ali , Nahla Sh. Ajeel , Hasanain Saad Alhares , Sabah J. Mohammed , Mohanad J. M-Ridha","doi":"10.1016/j.ibiod.2025.106223","DOIUrl":"10.1016/j.ibiod.2025.106223","url":null,"abstract":"<div><div>This study investigated phytoremediation of Congo red (CR) and methyl orange (MO) dye-contaminated water samples with <em>Ceratophyllum demersum</em> L. (coontail). Phytotoxicity assessments were also conducted to clarify the effects of dye concentrations on the growth and half-life of the plant. Based on the results, the 100 mg/L dye killed half of the plant (half-life) within 27 days. Increased dye concentration and exposure period also led to elevated toxicity in the plants. The results indicated that the dyes negatively affected plant growth. Conversely, incorporating NPK nutrients contributed to the plants overcoming toxicity and enhanced their growth. Furthermore, the MO dye exhibited fewer negative effects on the plant than the CR dye. Nevertheless, the phytoremediation process applied was successful. A 20 g of the coontail plant (dye solution volume = 5 L, initial pH = 7.0, initial dye level = 25 mg/L) dye removal percentages 100 % and 98 % CR and MO dyes during 15 days of exposure, respectively. FTIR data revealed that the various functional groups on the plant surface enabled coontail to absorb dyes from aqueous solutions, including carboxyl and carbonyl groups. Comparisons of control (without dye) and exposed (25 mg/L of initial dye concentration) coontail FESEM images demonstrated considerably altered morphological properties during phytoremediation of the dyes. These changes indicated effective loading of the dye onto the surfaces of the coontail samples. The uptake of dyes by coontail follows the Michaelis-Menten kinetics, indicating that adsorption occurs through a limited number of active sites on the plant's surface. Consequently, coontail is a notably efficient plant, applicable in eradicating dye-contaminated wastewater.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"207 ","pages":"Article 106223"},"PeriodicalIF":4.1,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145270883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-10-01DOI: 10.1016/j.ibiod.2025.106222
Lei Wu , Ming-Shu Zhang , Zhen-Dong Yang , Zhi-Wei Li , Jun-Jin Deng , Xiao-Chun Luo
Maintaining sulfur homeostasis is critical for cell viability. However, sulfide transmembrane transportation is not well revealed. This study identifies SsTauE, a CPA/AT family sulfite exporter in Streptomyces sp. SCUT-3, a potent feather degrader. sstauE and cysteine dioxygenase gene cdo1 form an operon up-regulated by cysteine. Overexpression of sstauE significantly enhances sulfite efflux and sulfite resistance. Site-directed mutagenesis reveals that Lys289 and Arg292, crucial for Lys-Arg-Val hydrogen bond bridge, are essential for core subdomains formation and function of SstauE. Co-overexpression with sstauE alleviates the sulfite toxicity from cdo1 overexpression alone, increases extracellular sulfite production 2.5-fold and facilitates feather degradation by enhancing disulfide bond breakdown, which increases 2.1 times total keratin amino acid and polypeptide recovery compared to wild-type at day 2. This study elucidates the coordinated regulation of sulfite production by CDO1 and efflux SsTauE in Streptomyces, providing insights into sulfur homeostasis and offering potential strategies for improved waste feather biodegradation.
{"title":"Sulfite exporter SsTauE enhances bacterial feather degradation by maintaining sulfur homeostasis","authors":"Lei Wu , Ming-Shu Zhang , Zhen-Dong Yang , Zhi-Wei Li , Jun-Jin Deng , Xiao-Chun Luo","doi":"10.1016/j.ibiod.2025.106222","DOIUrl":"10.1016/j.ibiod.2025.106222","url":null,"abstract":"<div><div>Maintaining sulfur homeostasis is critical for cell viability. However, sulfide transmembrane transportation is not well revealed. This study identifies SsTauE, a CPA/AT family sulfite exporter in <em>Streptomyces</em> sp. SCUT-3, a potent feather degrader. <em>sstauE</em> and cysteine dioxygenase gene <em>cdo1</em> form an operon up-regulated by cysteine. Overexpression of <em>sstauE</em> significantly enhances sulfite efflux and sulfite resistance. Site-directed mutagenesis reveals that Lys289 and Arg292, crucial for Lys-Arg-Val hydrogen bond bridge, are essential for core subdomains formation and function of SstauE. Co-overexpression with <em>sstauE</em> alleviates the sulfite toxicity from <em>cdo1</em> overexpression alone, increases extracellular sulfite production 2.5-fold and facilitates feather degradation by enhancing disulfide bond breakdown, which increases 2.1 times total keratin amino acid and polypeptide recovery compared to wild-type at day 2. This study elucidates the coordinated regulation of sulfite production by CDO1 and efflux SsTauE in <em>Streptomyces</em>, providing insights into sulfur homeostasis and offering potential strategies for improved waste feather biodegradation.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"207 ","pages":"Article 106222"},"PeriodicalIF":4.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145189614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-27DOI: 10.1016/j.ibiod.2025.106221
Peng Zhang , Bing Wang , Peichao Hu , Hui Liu , Junjie Tian , Guomin Li , Rao Fu , Jian Zhang
This study examines the influence of tourmaline (TM) supplementation on nitrogen retention, microbial functionality, and lignocellulose degradation during composting. TM application significantly reduced ammonia emissions and promoted nitrate accumulation by upregulating key nitrification genes (amoA, nxrA) while suppressing denitrification genes (nirS, norB, nosZ). TM exhibited superior nitrogen retention, primarily attributable to its strong NH3 adsorption during the thermophilic phase of composting (qTM = 0.9–2.4 mg g−1 TM d−1). Co-occurrence network analysis demonstrated that TM restructured microbial interactions by suppressing denitrifiers and enriching nitrifiers. Moreover, TM enhanced the activity of carbohydrate-active enzymes (CAZymes)—including GH51, AA3, GH16, and AA7—thereby expediting the degradation of cellulose and lignin. This process elevated the levels of fermentable sugars and facilitated the biosynthesis of amino acids, including L-lysine and L-aspartate. Collectively, these findings indicate that TM enhances microbial metabolic efficiency, accelerates compost maturation, and conserves nitrogen, thereby offering a promising strategy for high-efficiency composting.
{"title":"Effects of tourmaline additive on carbon and nitrogen metabolism dynamics during sludge composting","authors":"Peng Zhang , Bing Wang , Peichao Hu , Hui Liu , Junjie Tian , Guomin Li , Rao Fu , Jian Zhang","doi":"10.1016/j.ibiod.2025.106221","DOIUrl":"10.1016/j.ibiod.2025.106221","url":null,"abstract":"<div><div>This study examines the influence of tourmaline (TM) supplementation on nitrogen retention, microbial functionality, and lignocellulose degradation during composting. TM application significantly reduced ammonia emissions and promoted nitrate accumulation by upregulating key nitrification genes (<em>amoA</em>, <em>nxrA</em>) while suppressing denitrification genes (<em>nirS</em>, <em>norB</em>, <em>nosZ</em>). TM exhibited superior nitrogen retention, primarily attributable to its strong NH<sub>3</sub> adsorption during the thermophilic phase of composting (q<sub>TM</sub> = 0.9–2.4 mg g<sup>−1</sup> TM d<sup>−1</sup>). Co-occurrence network analysis demonstrated that TM restructured microbial interactions by suppressing denitrifiers and enriching nitrifiers. Moreover, TM enhanced the activity of carbohydrate-active enzymes (CAZymes)—including GH51, AA3, GH16, and AA7—thereby expediting the degradation of cellulose and lignin. This process elevated the levels of fermentable sugars and facilitated the biosynthesis of amino acids, including L-lysine and L-aspartate. Collectively, these findings indicate that TM enhances microbial metabolic efficiency, accelerates compost maturation, and conserves nitrogen, thereby offering a promising strategy for high-efficiency composting.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"206 ","pages":"Article 106221"},"PeriodicalIF":4.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-22DOI: 10.1016/j.ibiod.2025.106219
Diego Hernández-Ospina , Jean Viccari- Pereira , Carlos S. Osorio-González , Richard Martel , Satinder K. Brar
The increasing demand for petroleum hydrocarbons has led to rising levels of high-risk pollutants such as Benzene-Toluene-Ethylbenzene-Xylene (BTEX) in the environment, and widely recognized for their carcinogenic nature. This study evaluates the potential tolerance and the single and multi-compound degradation of BTEX compounds by a coculture of Serratia fonticola and Microbacterium esteraromaticum. Batch treatments were conducted in synthetic media, supplemented with 30 mg L−1 of benzene, xylene, toluene and ethylbenzene for a single compound degradation test and with 120 mg L−1 of BTEX compounds in a 1:1:1:1 ratio (equal weight-based contributions) for a multiple compound degradation test. Coculture showed a BTEX multicompound degradation of 47 %, which is 5 % and 2 % higher degradation than the one achieved by S. fonticola and M. esteraromaticum, respectively. Degradation for single-compound shows 99 % for benzene, 85 % for ethylbenzene, 72 % for toluene, and 62 % for xylene. These findings provide new insights into bacterial coculture interactions under mixed-contaminant stress, its biodegradation performance and provide a strong basis for developing tailored bioremediation strategies in both single-compound and multi-compound degradation of BTEX. Furthermore, the glucose consumption and BTEX tolerance could serve as potential indicators for monitoring of the progress and efficacy of BTEX remediation in contaminated environments.
{"title":"BTEX degradation by the coculture of S. fonticola and M. esteraromaticum","authors":"Diego Hernández-Ospina , Jean Viccari- Pereira , Carlos S. Osorio-González , Richard Martel , Satinder K. Brar","doi":"10.1016/j.ibiod.2025.106219","DOIUrl":"10.1016/j.ibiod.2025.106219","url":null,"abstract":"<div><div>The increasing demand for petroleum hydrocarbons has led to rising levels of high-risk pollutants such as Benzene-Toluene-Ethylbenzene-Xylene (BTEX) in the environment, and widely recognized for their carcinogenic nature. This study evaluates the potential tolerance and the single and multi-compound degradation of BTEX compounds by a coculture of <em>Serratia fonticola</em> and <em>Microbacterium esteraromaticum</em>. Batch treatments were conducted in synthetic media, supplemented with 30 mg L<sup>−1</sup> of benzene, xylene, toluene and ethylbenzene for a single compound degradation test and with 120 mg L<sup>−1</sup> of BTEX compounds in a 1:1:1:1 ratio (equal weight-based contributions) for a multiple compound degradation test. Coculture showed a BTEX multicompound degradation of 47 %, which is 5 % and 2 % higher degradation than the one achieved by <em>S. fonticola</em> and <em>M. esteraromaticum</em>, respectively. Degradation for single-compound shows 99 % for benzene, 85 % for ethylbenzene, 72 % for toluene, and 62 % for xylene. These findings provide new insights into bacterial coculture interactions under mixed-contaminant stress<u>,</u> its biodegradation performance and provide a strong basis for developing tailored bioremediation strategies in both single-compound and multi-compound degradation of BTEX. Furthermore, the glucose consumption and BTEX tolerance could serve as potential indicators for monitoring of the progress and efficacy of BTEX remediation in contaminated environments.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"206 ","pages":"Article 106219"},"PeriodicalIF":4.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-21DOI: 10.1016/j.ibiod.2025.106220
Ayesha Alam , Shafeeq Rahman , Labeeb Ali , Mohammednoor Altarawneh
Microplastics are notorious class of environmental pollutants that are added to the environment by the slow degradation of plastic infrastructure commonly used everywhere. Microplastics are small particles that tend to flow through the xylem vessels of plant roots and bioaccumulate in the plant tissues. Saline sandy soil in the United Arab Emirates (UAE) is colonized by beneficial bacterial strains that exhibit plastic degradation properties. This area is of great interest for new insights; however, very little is known about microplastic-degrading microorganisms, particularly in the Middle Eastern region of the world. To fill this gap, bacterial strains isolated from hypersaline soil offer a promising and sustainable approach to mitigate microplastic pollution in the form of granular biofertilizer and liquid cell suspension in soil and soilless crop production systems. The following study entails the relative potential of Bacillus subtilis and Halomonas meridiana, identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS), to mineralize plastics by forming biofilms. The study indicated the plastic biodegradation efficiency of the bacterial strains ranged from as low as 6.42 ± 5.73 % and as high as 16.95 ± 3.37 % in liquid and solid culture media, with an average of 10 % loss of polymer weight. The bacterial strains exhibited a strong ability of biofilms (optical density ≥0.3–0.5) and enzymatic activity (enzymatic units ≥0.006–0.01), confirmed by optical density spectrophotometric absorbances. Scanning Electron Microscopy/Energy Dispersive X-ray Spectrometry (SEM-EDS) and Fourier Transform Infrared Spectroscopy (FTIR-S) revealed the formation of minerals, oxidation, increased O/C ratios, and significant variation in the surface anatomy of plastic particles co-cultured with bacteria. The complete absence of the C-Cl peak as a result of FTIR and the highest O/C of (0.600) as a result of SEM-EDS suggested the high degradation efficiency of B. subtilis as compared to H. meridiana. These outcomes confirm the incidence of plastic degradation efficiency and biofilm formation ability of B. subtilis and H. meridiana in both the solid and liquid matrix, signifying their dual application as granular biofertilizer as well as cell suspension to minimize the traces of plastic particles in the agricultural production systems purifying the tropic level of the food chain.
{"title":"Bacillus subtilis and Halomonas meridiana isolated from saline sandy soils mediate the biodegradation of polyvinyl chloride (PVC) microplastics","authors":"Ayesha Alam , Shafeeq Rahman , Labeeb Ali , Mohammednoor Altarawneh","doi":"10.1016/j.ibiod.2025.106220","DOIUrl":"10.1016/j.ibiod.2025.106220","url":null,"abstract":"<div><div>Microplastics are notorious class of environmental pollutants that are added to the environment by the slow degradation of plastic infrastructure commonly used everywhere. Microplastics are small particles that tend to flow through the xylem vessels of plant roots and bioaccumulate in the plant tissues. Saline sandy soil in the United Arab Emirates (UAE) is colonized by beneficial bacterial strains that exhibit plastic degradation properties. This area is of great interest for new insights; however, very little is known about microplastic-degrading microorganisms, particularly in the Middle Eastern region of the world. To fill this gap, bacterial strains isolated from hypersaline soil offer a promising and sustainable approach to mitigate microplastic pollution in the form of granular biofertilizer and liquid cell suspension in soil and soilless crop production systems. The following study entails the relative potential of <em>Bacillus subtilis</em> and <em>Halomonas meridiana,</em> identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS), to mineralize plastics by forming biofilms. The study indicated the plastic biodegradation efficiency of the bacterial strains ranged from as low as 6.42 ± 5.73 % and as high as 16.95 ± 3.37 % in liquid and solid culture media, with an average of 10 % loss of polymer weight. The bacterial strains exhibited a strong ability of biofilms (optical density ≥0.3–0.5) and enzymatic activity (enzymatic units ≥0.006–0.01), confirmed by optical density spectrophotometric absorbances. Scanning Electron Microscopy/Energy Dispersive X-ray Spectrometry (SEM-EDS) and Fourier Transform Infrared Spectroscopy (FTIR-S) revealed the formation of minerals, oxidation, increased O/C ratios, and significant variation in the surface anatomy of plastic particles co-cultured with bacteria. The complete absence of the C-Cl peak as a result of FTIR and the highest O/C of (0.600) as a result of SEM-EDS suggested the high degradation efficiency of <em>B. subtilis</em> as compared to <em>H. meridiana.</em> These outcomes confirm the incidence of plastic degradation efficiency and biofilm formation ability of <em>B. subtilis</em> and <em>H. meridiana</em> in both the solid and liquid matrix, signifying their dual application as granular biofertilizer as well as cell suspension to minimize the traces of plastic particles in the agricultural production systems purifying the tropic level of the food chain.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"206 ","pages":"Article 106220"},"PeriodicalIF":4.1,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145099497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-18DOI: 10.1016/j.ibiod.2025.106218
Shuang Deng , Jiabin Wang , Wei Song , Lijuan Zhang , Di Cao , Liyang Li
Shale oil sludge, as a petroleum hydrocarbon pollutant, poses a serious threat to the environment. This study is the first to focus on the degradation of shale oil sludge from the Daqing Gulong Oilfield, aiming to establish an environmentally friendly, low-cost, and highly effective bioremediation method. This approach aims to reduce the cost of treating oil sludge, increase corporate profits, and achieve the goal of coordinated economic and environmental development. The experiment successfully constructed a composite bacterial consortium containing Acinetobacter calcoaceticus, Bacillus cereus, and Pseudomonas qingdaonensis. Under laboratory conditions, the degradation rate of petroleum hydrocarbons reached 91.47 %. The composite bacterial consortium can rapidly proliferate in shale oil sludge and become the dominant bacterial population, maintaining a stable microbial community structure in complex environments. In practical applications, it shows excellent degradation effects on shale oil sludge. The degradation rate of the composite bacterial strain can reach up to 59.9 % in practical applications. This research not only provides a new technical approach and bacterial resources for the remediation of shale oil sludge in the Daqing Gulong Oilfield but also holds significant theoretical and practical significance.
{"title":"Research on the construction of degradation bacterial communities for Daqing Gulong Oilfield and their application effects","authors":"Shuang Deng , Jiabin Wang , Wei Song , Lijuan Zhang , Di Cao , Liyang Li","doi":"10.1016/j.ibiod.2025.106218","DOIUrl":"10.1016/j.ibiod.2025.106218","url":null,"abstract":"<div><div>Shale oil sludge, as a petroleum hydrocarbon pollutant, poses a serious threat to the environment. This study is the first to focus on the degradation of shale oil sludge from the Daqing Gulong Oilfield, aiming to establish an environmentally friendly, low-cost, and highly effective bioremediation method. This approach aims to reduce the cost of treating oil sludge, increase corporate profits, and achieve the goal of coordinated economic and environmental development. The experiment successfully constructed a composite bacterial consortium containing <em>Acinetobacter calcoaceticus, Bacillus cereus</em>, and <em>Pseudomonas qingdaonensis</em>. Under laboratory conditions, the degradation rate of petroleum hydrocarbons reached 91.47 %. The composite bacterial consortium can rapidly proliferate in shale oil sludge and become the dominant bacterial population, maintaining a stable microbial community structure in complex environments. In practical applications, it shows excellent degradation effects on shale oil sludge. The degradation rate of the composite bacterial strain can reach up to 59.9 % in practical applications. This research not only provides a new technical approach and bacterial resources for the remediation of shale oil sludge in the Daqing Gulong Oilfield but also holds significant theoretical and practical significance.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"206 ","pages":"Article 106218"},"PeriodicalIF":4.1,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145099495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-09-18DOI: 10.1016/j.ibiod.2025.106217
Katarzyna Wojtowicz , Teresa Steliga , Joanna Brzeszcz , Janusz Fyda , Tomasz Skalski , Piotr Kapusta
The research was aimed at determining the efficiency of phytoremediation supported by bioaugmentation using a microbial consortium based on autochthonous bacteria and wild plants naturally growing in an area of natural petroleum seeps (Scirpus sylvaticus and Cirsium oleraceum),transplanted for the cleanup of hydrocarbon-contaminated soil. A six-month phytoremediation process resulted in a decrease in concentrations: TPH—from 4320 to 455–261 mg/kg dry mass, and PAHs—from 7.19 to 1.47–1.76 mg kg−1 dry mass. The greatest reductions were observed for n-C10–n-C21 (91.9–99.8 %) and naphthalene (85.3–87.4 %), and the lowest for n-C30–n-C36 (62.0–87.3 %) and 4–6-ring PAHs (44.0–76.3 %). Intense growth of roots and shoots was found in plants from bioaugmented soils. Toxicological studies performed using biotests with varying sensitivity (Ostracodtoxkit < Microtox < MARA) and phytotoxicity test (Phytotoxkit) indicated a decrease in toxicity levels after phytoremediation supported by bioaugmentation, which correlated with the drop in TPH and PAHs contents in the treated soil. The study also evaluated the structure of the microbial community in the remediated soil. The presence of bacteria in the rhizosphere accelerated the degradation of contaminants and increased plant tolerance to adverse environmental conditions. Additionally, changes in the community of soil ciliates were assessed, showingthat functional group responses, rather than species richness alone, may serve as sensitive indicators of soil recovery and ecological stability.This study highlights the novelty and ecological relevance of combining wild-adapted plants and microbial consortia for sustainable remediation, while also demonstrating the potential of ciliates as sensitive bioindicators of soil recovery.
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Pub Date : 2025-09-17DOI: 10.1016/j.ibiod.2025.106216
Siyao Hou, Han Wang, Jiameng Sun, Jing Liu, Manxu Bai, Wanli Zhang, Wanli Xing, Rundong Li
This study investigated effects of hydrothermal temperature and time on hydrothermal carbonization (HTC) of sewage sludge (SS) in terms of biochar properties, migration and transformation of heavy metals (HMs). The surface functional groups of biochar were greatly affected by hydrothermal temperature and the stretching vibration peak intensities of hydroxyl, carboxyl and aliphatic compound declined with the increased temperature. HMs concentrations in hydrothermal liquid were sorted in the order: Mn > Zn > Cr > Ni > Pb > Cu and those in biochar were Mn > Zn > Cu > Cr > Pb > Ni. HMs concentrations in biochar were much higher than those in hydrothermal liquid, indicating the significant enrichment of HMs in biochar. The enhanced hydrothermal temperature further actuated HMs enrichment in biochar to higher concentrations, but prolonging reaction time only led to slight fluctuations. The bioavailability of HMs in biochar was sorted in the order: Mn > Zn > Ni > Cu > Pb and Cr. More HMs in biochar existed in residual and oxidizable fractions of low bioavailability, indicating HTC treatment reduced the ecological risk of HMs. The hydrothermal temperature was the key factor affecting migration and transformation of HMs. Driven by high hydrothermal temperature, Zn, Ni and Mn elements in biochar transformed from water soluble, acid soluble and reducible fractions to oxidizable or residual fraction. The bioavailability of Cu element in biochar first enhanced then declined with the increasing hydrothermal temperature, but Pb and Cr elements were not sensitive to reaction condition change.
研究了水热温度和时间对污泥水热炭化（HTC）的生物炭特性、重金属迁移和转化的影响。生物炭表面官能团受水热温度影响较大，羟基、羧基和脂肪族化合物的拉伸振动峰强度随温度升高而降低。水热液中HMs浓度排序为Mn >； Zn > Cr > Ni > Pb > Cu；生物炭中HMs浓度排序为Mn >； Zn > Cu > Cr > Pb > Ni。生物炭中HMs的浓度远高于热液中，说明生物炭中HMs富集显著。水热温度的提高进一步促使生物炭中HMs的富集达到更高的浓度，但延长反应时间只会引起轻微的波动。生物炭中HMs的生物利用度排序为：Mn >； Zn > Ni > Cu >； Pb和Cr。生物炭中HMs较多存在于生物利用度较低的残余部分和可氧化部分，说明HTC处理降低了HMs的生态风险。热液温度是影响溶质迁移转化的关键因素。在高温水热作用下，生物炭中的锌、镍、锰元素由水溶性、酸溶性和可还原组分转化为可氧化组分或残余组分。随着水热温度的升高，生物炭中Cu元素的生物利用度先升高后降低，而Pb和Cr元素对反应条件的变化不敏感。
{"title":"Hydrothermal carbonization of sewage sludge: Effects of hydrothermal temperature and time on biochar properties and migration and transformation of heavy metals","authors":"Siyao Hou, Han Wang, Jiameng Sun, Jing Liu, Manxu Bai, Wanli Zhang, Wanli Xing, Rundong Li","doi":"10.1016/j.ibiod.2025.106216","DOIUrl":"10.1016/j.ibiod.2025.106216","url":null,"abstract":"<div><div>This study investigated effects of hydrothermal temperature and time on hydrothermal carbonization (HTC) of sewage sludge (SS) in terms of biochar properties, migration and transformation of heavy metals (HMs). The surface functional groups of biochar were greatly affected by hydrothermal temperature and the stretching vibration peak intensities of hydroxyl, carboxyl and aliphatic compound declined with the increased temperature. HMs concentrations in hydrothermal liquid were sorted in the order: Mn > Zn > Cr > Ni > Pb > Cu and those in biochar were Mn > Zn > Cu > Cr > Pb > Ni. HMs concentrations in biochar were much higher than those in hydrothermal liquid, indicating the significant enrichment of HMs in biochar. The enhanced hydrothermal temperature further actuated HMs enrichment in biochar to higher concentrations, but prolonging reaction time only led to slight fluctuations. The bioavailability of HMs in biochar was sorted in the order: Mn > Zn > Ni > Cu > Pb and Cr. More HMs in biochar existed in residual and oxidizable fractions of low bioavailability, indicating HTC treatment reduced the ecological risk of HMs. The hydrothermal temperature was the key factor affecting migration and transformation of HMs. Driven by high hydrothermal temperature, Zn, Ni and Mn elements in biochar transformed from water soluble, acid soluble and reducible fractions to oxidizable or residual fraction. The bioavailability of Cu element in biochar first enhanced then declined with the increasing hydrothermal temperature, but Pb and Cr elements were not sensitive to reaction condition change.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"206 ","pages":"Article 106216"},"PeriodicalIF":4.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145099578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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