International Biodeterioration & Biodegradation最新文献_第10页

Microbial remediation of polyethylene microplastic-tetracycline combined contamination in soil: Interactions and ecological implications 土壤中聚乙烯微塑料-四环素复合污染的微生物修复：相互作用和生态意义

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-07-21 DOI: 10.1016/j.ibiod.2025.106165

Huiyan Zhang, Xinyu Liu, Yuhan Gao, Yang Feng, Yang Wu

Microplastics bind organic/inorganic contaminants, forming complex composite pollution harder to remediate than isolated microplastics, particularly with antibiotics in soil. This study investigated the degradation mechanisms of four microorganisms (Aspergillus terreus, Aspergillus sydowii, Bacillus cereus and Lysinibacillus fusiformis) on composite contamination formed by polyethylene (PE) microplastics and tetracycline(TC). FTIR analysis showed new bands at 1700–1800 cm⁻¹ (indicating aldehyde, ketone, carboxylic acid) and a broad peak at 3400–3500 cm⁻¹ (suggesting hydrogen bonding or ketone formation), demonstrating polyethylene degraded and oxidized significantly by microorganisms, with tetracycline attached to PE microplastics. XRD indicated that tetracycline negatively affected microbial alteration of polyethylene's molecular structure. After identifying Lysinibacillus fusiformis as the dominant microorganism, further tests on PE after 32 days of microbial degradation by Lysinibacillus fusiformis revealed flaky surfaces with increased roughness, erosion, and crack deepening via SEM. GPC results showed increased Mn, Mw, and PD (43815, 129961, and 2.97, respectively) and indicated high molecular weight formation and low molecular weight degradation. Microbial population in composite-contaminated soil exhibited slow growth from 0 to 12 days, decline from 12 to 24 days, and stabilization thereafter. FDA enzyme activity decreased from 8 to 16 days and then gradually increased. Soil pH fluctuated between 7.20 and 7.70, while cation exchange capacity (CEC) decreased, with values below 10 cmol/kg for soils with three or more microbial strains, significantly reducing nutrient retention capacity. These findings show that pollutant addition altered soil properties and microbial living space, providing valuable insights into the biodegradation of microplastic composite contamination in soil.

微塑料结合有机/无机污染物，形成复杂的复合污染，比孤立的微塑料更难修复，特别是土壤中的抗生素。研究了四种微生物（土曲霉、西多曲霉、蜡样芽孢杆菌和梭状芽孢杆菌）对聚乙烯（PE）微塑料和四环素（TC）形成的复合污染物的降解机理。FTIR分析显示，在1700-1800 cm−1处有新的波段（表明醛、酮、羧酸），在3400-3500 cm−1处有一个宽峰（表明氢键或酮的形成），表明聚乙烯被微生物降解和氧化，四环素附着在PE微塑料上。XRD分析表明，四环素对聚乙烯分子结构的微生物改变有负面影响。在确定梭状芽胞杆菌为优势微生物后，对PE进行了32天的微生物降解后的进一步测试，通过扫描电镜发现，表面呈片状，粗糙度增加，侵蚀，裂缝加深。GPC结果显示Mn、Mw和PD增加（分别为43815、129961和2.97），表明高分子量形成和低分子量降解。复合污染土壤微生物数量在0 ~ 12 d呈缓慢增长，12 ~ 24 d呈下降趋势，之后趋于稳定。8 ~ 16 d， FDA酶活性下降，然后逐渐升高。土壤pH值在7.20 ~ 7.70之间波动，阳离子交换容量（CEC）下降，有3种或更多微生物菌株的土壤CEC值低于10 cmol/kg，显著降低了养分保持能力。这些发现表明，污染物的添加改变了土壤性质和微生物的生存空间，为土壤中微塑料复合材料污染的生物降解提供了有价值的见解。

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引用次数: 0

Improving compost nutrient composition and microbial community structure through Bacillus licheniformis 利用地衣芽孢杆菌改善堆肥营养成分和微生物群落结构

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-07-19 DOI: 10.1016/j.ibiod.2025.106164

Xufeng Dou , Xiangshuo Zeng , Hao Tang , Yuxia Mei , Haihong Jiao , Min Ren

Spent Mushroom Substrate (SMS) from mushroom cultivation is an agricultural waste with potential for resource utilization. Fermentation of waste mushroom sachets by Bacillus licheniformis has not been adequately studied for its effect on nutrient content and microbial composition. In this study, a strain of Bacillus licheniformis TRM58488 was isolated from Pamir Plateau soil. The strain is safe, and its effects on co-composting spent mushroom substrate (SMS) with chicken manure were further evaluated. After inoculation with the strain in the experimental group, the content of nutrients such as nitrogen, phosphorus, and potassium in the compost has changed, among which the effective potassium content has increased by 73.17 %, the organic matter and humic acid content was reduced, and the microbial community structure was significantly changed, with the abundance of degrading functional phyla, such as Actinomycetota and Pseudomonadota, increased. The study showed that this strain can optimize the nutrient composition and microbial composition of compost, but its safety and long - term fertilizer efficiency need to be further verified by extending the fermentation time, adding high - carbon auxiliaries and field trials. The study provides new options of microbial agents for resource utilization of agricultural wastes, while emphasizing the importance of ecological risk assessment of compost.

食用菌废基质是一种具有资源化利用潜力的农业废弃物。地衣芽孢杆菌发酵废香菇香囊对其营养成分和微生物组成的影响尚未得到充分的研究。本研究从帕米尔高原土壤中分离到一株地衣芽孢杆菌TRM58488。该菌株是安全的，并进一步评价了其在废菌基质与鸡粪共堆肥中的效果。接种该菌株后，试验组堆肥中氮、磷、钾等营养物质含量发生变化，其中有效钾含量提高73.17%，有机质和腐殖酸含量降低，微生物群落结构发生显著变化，放线菌门、假单胞菌门等降解功能门丰度增加。研究表明，该菌株可以优化堆肥的营养成分和微生物组成，但其安全性和长期肥效需要通过延长发酵时间、添加高碳助剂和田间试验进一步验证。该研究为农业废弃物资源化利用提供了新的微生物制剂选择，同时强调了堆肥生态风险评价的重要性。

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引用次数: 0

Distribution and control of the pest darkling beetles (Coleoptera, Tenebrionidae) at the world heritage site Mogao Grottoes 世界遗产地莫高窟黑甲（鞘翅目，拟甲科）的分布及防治

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-07-19 DOI: 10.1016/j.ibiod.2025.106161

Feiqing Zhu , Fasi Wu , Yuxin Chen , Jie Li , Guobin Zhang , Long Li , Zhang Chen

To explore the spatiotemporal distribution of darkling beetles (Coleoptera, Tenebrionidae) in the Mogao Grottoes, 26 sampling points were selected at the top of the caves from south to north. From 2022 to 2023, the trap method was employed to investigate the darkling beetle community. Canonical correspondence analysis was utilized to analyze the relationship between the number of adult darkling beetles and various meteorological factors. Twelve caves with frequently occurring darkling beetles were selected and paired with 12 out of the 26 monitoring sampling points. The spatial distribution of darkling beetles was investigated from May to July 2022. Physical, chemical, and biological control experiments were proposed to explore effective control methods for darkling beetles. A total of 10,596 specimens in 5 genera and 5 species were obtained. The peak period of darkling beetles at the top of the caves is from May to July. The dominant species, Pterocoma loczyi, and Colposcelis trisulcata, peak from April to July, with the highest in May. Solar radiation and precipitation are the main meteorological factors influencing the occurrence of darkling beetles. Regarding the spatial distribution of darkling beetle numbers, the insects in the southern section are positively correlated with the corresponding caves, unobvious relationship in the northern section. Moreover, the physical, chemical, and biological control methods all displayed significant effects on eliminating the darkling beetles with high kill rates. The above research results provide fundamental data for establishing a green and sustainable control system aimed at cultural heritage pests.

为探讨莫高窟暗甲（鞘翅目，拟甲科）的时空分布，从南向北选取了26个洞顶采样点。2022 - 2023年，采用诱捕法对黑甲群落进行调查。采用典型对应分析方法，分析了各气象因子与成虫数量的关系。在26个监测采样点中，选择了12个经常发生黑甲虫的洞穴，并将其与12个配对。于2022年5月至7月对暗甲虫的空间分布进行了调查。提出了物理、化学和生物防治实验，探索有效的防治方法。共获得5属5种10596份标本。5月至7月是洞顶黑甲虫活动的高峰期。4 ~ 7月为优势种，以斑点斑蝶（Pterocoma loczyi）和三叶草（Colposcelis trisulata）居多，5月最高。太阳辐射和降水是影响暗甲虫发生的主要气象因子。在暗甲虫数量的空间分布上，南段暗甲虫数量与相应洞穴数量呈正相关，北段暗甲虫数量关系不明显。此外，物理、化学和生物防治方法均对杀虫率高的黑甲有显著的杀灭效果。以上研究结果为建立绿色可持续的文物有害生物防治体系提供了基础数据。

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引用次数: 0

Detection of a conserved multi-heme cytochrome gene cluster in severely corrosive sulfate-reducing biofilms 在严重腐蚀硫酸盐还原生物膜中检测保守的多血红素细胞色素基因簇

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-07-15 DOI: 10.1016/j.ibiod.2025.106154

Sven Lahme, Jaspreet Mand , John Longwell, Dennis Enning

Sulfate-reducing bacteria (SRB), often considered primary culprits of microbially influenced corrosion (MIC), can be highly abundant in engineered anoxic environments such as oil and gas pipelines. The marine lithotrophic SRB Desulfovibrio ferrophilus strain IS5 is increasingly viewed as a model organism for anaerobic steel degradation due to its unrivalled ability to cause severe corrosion in laboratory tests where Fe⁰ provides the only electron donor. Recent studies suggest that multi-heme c-type cytochromes may be involved in the oxidation of Fe⁰ by this strain. We simulated oilfield MIC in bioreactors under sulfate-reducing conditions and observed material degradation rates ranging from negligible (0.01 mm Fe⁰ yr⁻¹) to severe corrosion (2.75 mm Fe⁰ yr⁻¹). DNA shotgun sequencing of severely corrosive mixed microbial communities revealed metagenome-assembled genomes of the Desulfovibrionaceae and Desulfobulbaceae families that contained a similar multi-heme cytochrome gene cluster like strain IS5. This prompted the development of primers and probes for the quantification of a c-type cytochrome in the conserved cluster with a proposed key function in MIC. The so-called micC gene was indeed detectable in all corrosive cultures yet absent in mixed SRB communities that failed to affect steel integrity. We further demonstrated the practical utility of the novel qPCR assay by surveying oil and gas infrastructure across different geographic locations. Here, micC was only detected in those assets with a history of MIC. The systematic quantification of genetic determinants underlying steel corrosion by lithotrophic microorganisms could transform microbial monitoring strategies, allowing for the proactive identification of critical MIC events in industrial applications.

硫酸盐还原菌（SRB）通常被认为是微生物影响腐蚀（MIC）的罪魁祸首，在石油和天然气管道等工程缺氧环境中可能非常丰富。海洋岩石营养SRB嗜铁Desulfovibrio菌株IS5越来越被视为厌氧钢降解的模式生物，因为它在实验室测试中具有无可比拟的严重腐蚀能力，在实验室测试中，Fe0是唯一的电子供体。最近的研究表明，多血红素c型细胞色素可能参与了该菌株对Fe0的氧化。我们在硫酸盐还原条件下的生物反应器中模拟了油田MIC，观察到材料的降解率从微不足道（0.01 mm Fe0 yr - 1）到严重腐蚀（2.75 mm Fe0 yr - 1）不等。对严重腐蚀混合微生物群落的DNA散弹枪测序显示，Desulfovibrionaceae和Desulfobulbaceae家族的宏基因组组装基因组包含与菌株IS5相似的多血红素细胞色素基因簇。这促使了引物和探针的发展，用于定量在MIC中具有关键功能的保守簇中的c型细胞色素。所谓的micC基因确实在所有腐蚀性培养物中都可以检测到，但在混合SRB群落中却没有检测到，这未能影响钢的完整性。我们通过调查不同地理位置的石油和天然气基础设施，进一步证明了新型qPCR检测的实用性。这里，micC仅在那些有micC历史的资产中检测到。系统地量化岩石营养微生物对钢铁腐蚀的遗传决定因素可以改变微生物监测策略，允许在工业应用中主动识别关键的MIC事件。

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引用次数: 0

Panchagavya-enhanced microbial biodegradation of steel slag: A sustainable phytoremediation approach panchagavya增强的钢渣微生物降解：一种可持续的植物修复方法

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-07-15 DOI: 10.1016/j.ibiod.2025.106162

AnjaniKumar S.V. Brahmandam , Khanindra Pathak , Varaprasad Kasa , Brajesh Kumar Dubey

Industrial steel slag (SS) is a challenging environmental pollutant, and its stockpiling results in areas with high pH, salinity, heavy metal content, and poor fertility. These properties render stockpiled SS sites inhospitable to natural plant colonization, creating a serious obstacle for land restoration. Current remediation methods are inadequate, warranting the development of innovative and sustainable strategies for the effective reclamation of such degraded lands. This study explores the use of Panchagavya (PG), a traditional organic formulation packed with nutrients, bioactive compounds, and beneficial microbes, as a groundbreaking amendment for phytoremediation of such sites. By combining PG with Sesbania grandiflora, a resilient plant species, we aimed to convert SS from a problematic by-product, into a resource for ecological recovery. Comprehensive chemical and biological characterizations of PG were performed, followed by pot studies using S. grandiflora grown under various SS concentrations (0 %–100 %). Results showed that PG successfully neutralizes alkaline stress, stimulates microbial activity, and enhances nutrient availability, significantly improving plant growth. Notably, the combination of PG and moderate SS concentrations (50 %) led to the highest growth rates, facilitated by increased microbial diversity and enzyme activity, which improved nutrient cycling and mitigated stress. Metagenomic analysis revealed substantial enrichment of beneficial bacteria, such as Sphingomonas and Bacillus, which are known for promoting plant growth, nutrient cycling, and pollutant degradation. The bioactive compounds present in PG further enhanced plant resilience and nutrient uptake. Our findings establish PG as a powerful, cost-effective, and scalable solution for phytoremediation of SS-contaminated sites.

工业钢渣（SS）是一种具有挑战性的环境污染物，在pH、盐度、重金属含量高、肥力差的地区积存。这些特性使得储存的SS站点不适合自然植物殖民，给土地恢复造成严重障碍。目前的补救方法是不够的，需要制定创新和可持续的战略，以便有效地复垦这种退化的土地。本研究探索了Panchagavya （PG）的使用，这是一种传统的有机配方，富含营养物质，生物活性化合物和有益微生物，作为植物修复这些场所的开创性修正。通过将甘油三酯（PG）与大田葵（Sesbania grandflora）结合，我们旨在将甘油三酯从有问题的副产品转化为生态恢复的资源。对PG进行了全面的化学和生物学表征，然后在不同SS浓度（0% - 100%）下进行了盆栽研究。结果表明，PG能有效中和碱性胁迫，刺激微生物活性，提高养分有效性，显著促进植物生长。值得注意的是，PG和中等SS浓度（50%）的组合导致了最高的生长速率，促进了微生物多样性和酶活性的增加，从而改善了养分循环并减轻了胁迫。宏基因组分析显示，有益菌如鞘氨单胞菌和芽孢杆菌大量富集，这些有益菌以促进植物生长、养分循环和污染物降解而闻名。PG中存在的生物活性化合物进一步增强了植物的抗逆性和养分吸收。我们的研究结果表明，PG是一种强大的、具有成本效益的、可扩展的植物修复ss污染场地的解决方案。

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引用次数: 0

Monitoring colonization dynamics and photosynthetic activity of lichens in the House of the Ancient Hunt, Pompeii (Italy), under the climate change scenario 气候变化情景下意大利庞贝古猎屋地衣的定植动态和光合活性监测

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-07-14 DOI: 10.1016/j.ibiod.2025.106159

Marta Cicardi , Mariagrazia Morando , Laura Guidorzi , Veronica Bellacicco , Simone Guion , Enrica Matteucci , Greta Rao-Torres , Alessia Romano , Arianna V. Scarcella , Daniele Castelli , Alessandro Lo Giudice , Valeria Meirano , Alessandro Re , Marco Serino , Diego Elia , Sergio E. Favero-Longo

Strategies to prevent (re-)growths on heritage surfaces are required to improve the management of biodeterioration issues. Nevertheless, (re-)colonization dynamics may be also limited by the divergence of current (micro-)climatic conditions from those of the past which allowed biomass accumulation, as modelized for the Mediterranean area. In this work, lichen communities were re-surveyed after eight years on the vertical walls of the House of the Ancient Hunt in Pompeii (VII,4,48) (S-Italy), displaying shifts in cover values varying for species and the cardinal aspect of walls. In particular, the hygrophytic Dirina massiliensis showed a regression on the S-facing wall, while the xerophytic Verrucaria macrostoma relatively increased and the mesophytic Lepraria lobificans was rather unchanged. To evaluate if these trends reflect a change in the wall microclimatic suitability, the photosynthetic activity of lichens (and of phototrophic biofilms as a comparison) was fluorimetrically monitored across the four seasons, under changing meteorological conditions, at different cardinal aspects and distances from the ground. GLM models indicated a maximum influence of spatial related factors (primary wall aspect) on the variability of basal fluorescence (F₀) and maximum quantum yield (F_v/F_m), higher than that of lithobiont type and the temporal factors (month, interval from sunrise). Relationships between photosynthetic activity and meteorological conditions also showed aspect dependence, with rainfall and consequent wind-driven rain (WDR) having remarkable importance, particularly for S-aspect. On this aspect, the hygrophytic D. massiliensis showed prolonged photosynthetic inactivity, even under WDR favourable conditions, suggesting the loss of suitability of its current micro-niche and realizing expectations of climate change models.

防止（再）生长在遗产表面的策略是必要的，以改善生物恶化问题的管理。然而，（再）殖民化动态也可能受到当前（微）气候条件与过去允许生物量积累的气候条件的差异的限制，如地中海地区的模型所示。在这项工作中，地衣群落在庞贝古狩猎屋（意大利南部）的垂直墙壁上进行了8年后的重新调查，显示了物种和墙壁主要方面的覆盖值变化。其中，s型壁上的湿生植物马塞利草（Dirina massiliensis）出现了退行，而旱生植物大瘤菌（Verrucaria macrostoma）相对增加，中生植物大叶麻草（Lepraria loificans）基本没有变化。为了评估这些趋势是否反映了壁面小气候适宜性的变化，在四季变化的气象条件下，在不同的主要方面和离地面的距离上，用荧光法监测了地衣（和光养生物膜作为比较）的光合活性。GLM模型显示，空间相关因子（主壁向）对基底荧光（F0）和最大量子产率（Fv/Fm）的影响最大，高于岩石生物类型和时间因素（月份、日出间隔）。光合作用与气象条件的关系也表现出向依赖性，其中降雨和后续风驱雨（WDR）的重要性显著，尤其是s向。在这方面，即使在WDR有利条件下，湿生植物d.m asiliensis也表现出长时间的光合不活性，表明其当前微生态位的适宜性丧失，实现了气候变化模型的预期。

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引用次数: 0

Dynamic characteristics of attachment genes and their role in aerobic granular sludge development 附着基因的动态特性及其在好氧颗粒污泥发育中的作用

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-07-09 DOI: 10.1016/j.ibiod.2025.106160

Jia Liu , Xuan Hu , Yi Yun Gao , Jianrong Zhu

Aerobic granular sludge (AGS) is a microbial aggregate with a biofilm structure. Understanding microbial attachment at the genetic level is crucial for elucidating the potential mechanisms underlying AGS biofilm formation. This study applied RT‒qPCR to assess the correlation between key attachment genes (rmlA, rpfF, and fliD) and granulation. Comparative analysis of different sludge structures revealed that the abundances of attachment genes were 1–2 orders of magnitude greater in mature AGS than in floccular sludge. This indicated that the degree of microbial aggregation increased in parallel with the abundance of these attachment genes. Furthermore, the presence and dynamic characteristics of three key attachment genes showed significant positive correlations with granulation, particularly granule size (rmlA: r = 0.91, p < 0.001; rpfF: r = 0.88, p < 0.01, fliD: r = 0.97, p < 0.001). Dynamic quantification of attachment gene expression during AGS cultivation revealed stage-specific dominance: rpfF facilitated early-stage aggregation via quorum sensing, whereas fliD exhibited a steep increase (from 1.6 × 10³ to 2.56 × 10⁵ copies·(g·SS)⁻¹) during AGS stabilization, surpassing rmlA (1.47 × 10⁴ copies·(g·SS)⁻¹ and rpfF (1.12 × 10⁵ copies·(g·SS)⁻¹) during maturation. Bioaugmentation with Stenotrophomonas AGS-1 increased attachment gene amplification by 2–3 log units while competitively excluding low-abundance microbial groups and selectively enriching functionally dominant consortia. High-attachment bacteria may mediate AGS formation via attachment gene expression. This study revealed aerobic granulation mechanisms as a gene-driven bacterial aggregation process for the first time, highlighting the role of attachment genes in AGS development and providing guidance on biofilm regulation in AGS technology.

好氧颗粒污泥（AGS）是一种具有生物膜结构的微生物集合体。在遗传水平上理解微生物附着对于阐明AGS生物膜形成的潜在机制至关重要。本研究采用RT-qPCR技术评估关键附着基因（rmlA、rpfF和fliD）与肉芽的相关性。不同污泥结构的对比分析表明，成熟AGS中附着基因的丰度比絮凝污泥高1-2个数量级。这表明微生物聚集的程度与这些附着基因的丰度平行增加。此外，3个关键附着基因的存在及其动态特性与颗粒大小呈显著正相关(rmlA: r = 0.91, p <；0.001;rpfF: r = 0.88, p <；0.01, fliD: r = 0.97, p <；0.001)。在AGS培养过程中，对附着基因表达的动态定量分析显示了阶段特异性优势：rpfF通过群体感应促进了早期的聚集，而在AGS稳定过程中，fliD的数量急剧增加（从1.6 × 103增加到2.56 × 105拷贝·（g·SS） - 1），在成熟过程中超过了rmlA (1.47 × 104拷贝·（g·SS） - 1和rpfF （1.12 × 105拷贝·（g·SS） - 1）。用窄养单胞菌AGS-1进行生物强化，可使附着基因扩增量增加2-3个对数单位，同时竞争性地排除低丰度微生物群，选择性地富集功能优势菌群。高附着细菌可能通过附着基因的表达介导AGS的形成。本研究首次揭示了好氧肉芽形成机制是一种基因驱动的细菌聚集过程，突出了附着基因在AGS发育中的作用，为AGS技术中的生物膜调控提供了指导。

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引用次数: 0

Substrate dynamics on anammox inhibition during domestic wastewater treatment under controlled and ambient temperature 在可控温度和环境温度下，生活污水处理过程中基质抑制厌氧氨氧化的动态研究

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-06-30 DOI: 10.1016/j.ibiod.2025.106158

Divyesh Parde, Ankit Singh, Manaswini Behera, Rajesh Roshan Dash

This study investigates the kinetic behavior and operational performance of an Anammox-based Moving Bed Biofilm Reactor (MBBR) treating synthetic and real domestic wastewater under varying COD/N ratios and hydraulic retention times (HRTs). The objective was to evaluate substrate removal kinetics for total nitrogen (TN) and chemical oxygen demand (COD), assess model applicability, assess and quantify the rates of reactions and identify constraints affecting anammox activity during real domestic wastewater treatment. Three kinetic models: first-order, Grau's second-order, and modified Stover–Kincannon were applied to quantify COD and TN removal. Results showed that while the first-order model provided limited accuracy (R² = 0.36 for COD), both the Grau's model (R² > 0.98) and Stover–Kincannon model (R² > 0.98) demonstrated strong predictive performance for both COD and TN removal. The reactor achieved up to 96 % TN removal and COD removal as high as 92 %, at 24 h HRT. However, higher COD/N ratios (>8) substantially suppressed Specific Anammox Activity (SAA), with up to 92 % inhibition at COD/N = 15, especially under ambient conditions. Comparisons of performance with synthetic and real domestic wastewater further revealed the impact of environmental fluctuations and complex organics on system stability, with TN removal declining from 89 % to 82 % from controlled to ambient scenarios. The study underscores the importance of COD/N ratio management, operational flexibility, and model-guided reactor design in sustaining anammox performance. These findings offer new insights into optimizing Anammox-MBBR reactor for synthetic and real domestic wastewater treatment in decentralized wastewater treatment applications.

研究了厌氧氨氧化移动床生物膜反应器（MBBR）在不同COD/N比和水力停留时间（HRTs）下处理合成废水和实际生活废水的动力学行为和运行性能。目的是评估总氮（TN）和化学需氧量（COD）的底物去除动力学，评估模型的适用性，评估和量化反应速率，并确定在实际生活污水处理过程中影响厌氧氨氧化活性的制约因素。采用一阶、Grau’s二阶和改进的Stover-Kincannon三种动力学模型对COD和TN的去除进行量化。结果表明，虽然一阶模型提供了有限的精度（R2 = 0.36），但Grau模型(R2 >；0.98)和stov - kincannon模型(R2 >；0.98)对COD和TN的去除率具有较强的预测性能。反应器在24 h HRT下TN去除率高达96%，COD去除率高达92%。然而，较高的COD/N比率（>8）大大抑制了特定厌氧氨氧化活性（SAA），在COD/N = 15时，特别是在环境条件下，抑制率高达92%。与合成废水和真实生活废水的性能比较进一步揭示了环境波动和复杂有机物对系统稳定性的影响，在受控和环境情景下，TN去除率从89%下降到82%。该研究强调了COD/N比管理、操作灵活性和模型引导反应器设计在维持厌氧氨氧化性能中的重要性。这些发现为优化Anammox-MBBR反应器在分散式污水处理应用中的合成和实际生活污水处理提供了新的见解。

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引用次数: 0

Sulphate reducing bacteria influenced corrosion of a low carbon steel in marine environment: the role of dissolved organic carbon and temperature 硫酸盐还原菌对海洋环境中低碳钢腐蚀的影响：溶解有机碳和温度的作用

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-06-27 DOI: 10.1016/j.ibiod.2025.106156

Michael Olatunde , Alfred R. Akisanya , Karen P. Scott , Jennifer C. Martin , M. Amir Siddiq , Srinivas Sriramula , Alethea Madgett

Sulphate Reducing Bacteria (SRB) are significant contributors to corrosion in marine environments. The corrosion induced by SRB can be influenced by the concentration of Dissolved Organic Carbon (DOC) in the media. In this study, we experimentally investigated the synergistic effects of DOC, temperature, and exposure time on the corrosion rate and morphology of a low-carbon steel in seawater containing SRB. The results showed that the corrosion rate was inversely proportional to the DOC concentration up to 25 days, but directly proportional for the subsequent 100 days of exposure, up to 125 days. There was no significant effect of temperatures between 15 and 25 °C on the corrosion rate. Localised corrosion activity of the SRB was found to be influenced by the heterogeneity of porous biofilm-corrosion product matrix on the steel surface.

硫酸盐还原菌（SRB）是海洋环境腐蚀的重要贡献者。介质中溶解有机碳（DOC）的浓度对SRB腐蚀的影响较大。在本研究中，我们通过实验研究了DOC、温度和暴露时间对含SRB海水中低碳钢腐蚀速率和形貌的协同效应。结果表明，在25天内，腐蚀速率与DOC浓度成反比，但在随后的100天内，腐蚀速率与DOC浓度成正比，达到125天。温度在15 ~ 25℃之间对腐蚀速率没有显著影响。发现SRB的局部腐蚀活性受多孔生物膜-腐蚀产物基质在钢表面的不均匀性的影响。

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A laboratory approach to mitigating ammonia in broiler litter material with chemical additives 用化学添加剂减少肉鸡窝料中氨的实验室方法

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-06-27 DOI: 10.1016/j.ibiod.2025.106157

Busra Yayli, Ilker Kilic

Broiler litter is a major source of ammonia (NH₃) emissions in poultry production systems, negatively impacting both animal welfare and worker health. This study aimed to evaluate the effectiveness of chemical additives in reducing NH₃ volatilization and to identify the optimal application dosage for practical use. The research was conducted in two phases. In Phase I, four additives — aluminum sulfate (Al₂(SO₄)₃), clinoptilolite zeolite, sodium bisulfate (NaHSO₄), and magnesium sulfate (MgSO₄) — were applied to spent broiler litter. Among them, NaHSO₄ demonstrated the highest NH₃ reduction efficiency (76.7 %). In Phase II, varying dosages of sodium bisulfate (2.5 %, 5 %, 7.5 %, and 10 % w/w) were tested, with the 7.5 % application achieving the highest mitigation performance (95.0 %). Additionally, sodium bisulfate treatment improved the ammonium nitrogen content of the litter, enhancing its agronomic value as a potential organic fertilizer. The findings highlight sodium bisulfate as a promising strategy for reducing NH₃ emissions, offering both environmental and economic benefits. This approach may enhance air quality in poultry houses. It can also increase the nutrient value of litter, contributing to sustainable crop production. The laboratory-scale results offer a strong basis for future field studies. These studies are needed to validate the method under commercial production conditions.

肉鸡产仔是家禽生产系统中氨（NH3）排放的主要来源，对动物福利和工人健康产生负面影响。本研究旨在评价化学添加剂减少NH3挥发的效果，并确定实际应用的最佳用量。研究分两个阶段进行。在第一阶段，将硫酸铝（Al2(SO4)3）、沸石沸石、硫酸氢钠（NaHSO4）和硫酸镁（MgSO4） 4种添加剂应用于废肉鸡窝产仔。其中NaHSO4的NH3还原效率最高（76.7%）。在第二阶段，测试了不同剂量的硫酸氢钠（2.5%、5%、7.5%和10% w/w），其中7.5%的应用达到了最高的缓解性能（95.0%）。此外，硫酸钠处理提高了凋落物的铵态氮含量，提高了其作为潜在有机肥的农艺价值。研究结果强调，硫酸氢钠是一种很有前途的减少NH3排放的策略，既能带来环境效益，又能带来经济效益。这种方法可以改善禽舍的空气质量。它还可以增加凋落物的营养价值，有助于可持续作物生产。实验室规模的结果为未来的实地研究提供了坚实的基础。这些研究需要在商业生产条件下验证该方法。

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