Pub Date : 2024-10-07DOI: 10.1016/j.ibiod.2024.105936
Vijendra Shah , Achlesh Daverey
In present study, response surface methodology (RSM) based central composite design (CCD) was used to study the interactive effect between two individual soil amendments, sophorolipids (SLs) and pine needle biochar for the phytoremediation of Cd contamination and enzymatic activity of soil. The experimented concentrations of SLs and biochar ranged from 0.5 to 2.6 g/kg and 0.4–1.8 %, respectively. The SLs and biochar at the concentration 1.1 g/kg and 1.55 % respectively exhibit the maximum uptake in shoot (125.33 mg/kg) and root (298.27 mg/kg). The results also showed higher R2 (>0.9) for Cd uptake in B. pilosa and R2 (>0.85) for soil enzymatic activity using model generated by CCD of RSM. These results signify the reliability of the model and suggested that this model could be used for the prediction of increased metal uptake by plants. The optimum concentrations of SLs and biochar predicted by the CCD were 1.23 g/kg and 1.55 %, respectively with desirability score of 1 for the uptake of Cd in B. pilosa. The results highlighted that application of these amendments can be a pivotal step in the direction of remediation of heavy metal contamination from soil at larger scale.
{"title":"Assessment of interactive effects of sophorolipids and pine needle biochar augmentation in the process of phytoremediation of Cd contaminated soil using response surface methodology","authors":"Vijendra Shah , Achlesh Daverey","doi":"10.1016/j.ibiod.2024.105936","DOIUrl":"10.1016/j.ibiod.2024.105936","url":null,"abstract":"<div><div>In present study, response surface methodology (RSM) based central composite design (CCD) was used to study the interactive effect between two individual soil amendments, sophorolipids (SLs) and pine needle biochar for the phytoremediation of Cd contamination and enzymatic activity of soil. The experimented concentrations of SLs and biochar ranged from 0.5 to 2.6 g/kg and 0.4–1.8 %, respectively. The SLs and biochar at the concentration 1.1 g/kg and 1.55 % respectively exhibit the maximum uptake in shoot (125.33 mg/kg) and root (298.27 mg/kg). The results also showed higher <em>R</em><sup><em>2</em></sup> (>0.9) for Cd uptake in <em>B. pilosa</em> and <em>R</em><sup><em>2</em></sup> (>0.85) for soil enzymatic activity using model generated by CCD of RSM. These results signify the reliability of the model and suggested that this model could be used for the prediction of increased metal uptake by plants. The optimum concentrations of SLs and biochar predicted by the CCD were 1.23 g/kg and 1.55 %, respectively with desirability score of 1 for the uptake of Cd in <em>B. pilosa</em>. The results highlighted that application of these amendments can be a pivotal step in the direction of remediation of heavy metal contamination from soil at larger scale.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105936"},"PeriodicalIF":4.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-07DOI: 10.1016/j.ibiod.2024.105933
Xueting Wang , Shihao Liu , Xiaoke Ding , Lele Zhang , Xueru Lv , Jie Li , Caihong Song , Chen Zhang , Shenghui Wang
The aromatic compound p-cresol is a notorious industrial pollutant characterized by its high toxicity, persistence and bioaccumulation within higher organisms. A thorough understanding of the microbial metabolic pathways involved in p-cresol degradation is critical for the design and optimization of microbial wastewater treatment systems. Despite numerous studies on the degradation pathways of p-cresol by various microbial species, the metabolic diversity within a single strain remains largely unexplored. This study investigated the metabolic mechanism of p-cresol in Bacillus subtilis ZW, a bacterium capable of degrading p-cresol. Through LC-MS analysis, we identified twelve distinct metabolic intermediates in the culture of strain ZW, leading to the proposal of three plausible degradation pathways. These include methyl hydroxylation, direct aromatic ring hydroxylation, and phosphorylation of the hydroxyl group, and all of which may concurrently contribute to p-cresol biodegradation by strain ZW. Further study showed that the genome of strain ZW harbored 47 coding genes associated with the degradation of p-cresol and its structural analogs, underscoring the metabolic versatility of this strain and its potential for xenobiotic biodegradation. These findings contribute valuable insights into the biodegradation mechanisms of pollutants. Under optimal degradation conditions of 35 °C and pH 7.0, strain ZW demonstrated the capacity to metabolize 27.5 % of p-cresol (10 mg/L) in minimal salt media within a week, and was capable of completely degrading 10 mg/L p-cresol in wastewater within five days. The potential utility of strain ZW in the bioremediation of p-cresol and other aromatic compounds is thus evident.
{"title":"Coexistence of diverse metabolic pathways promotes p-cresol biodegradation by Bacillus subtilis ZW","authors":"Xueting Wang , Shihao Liu , Xiaoke Ding , Lele Zhang , Xueru Lv , Jie Li , Caihong Song , Chen Zhang , Shenghui Wang","doi":"10.1016/j.ibiod.2024.105933","DOIUrl":"10.1016/j.ibiod.2024.105933","url":null,"abstract":"<div><div>The aromatic compound p-cresol is a notorious industrial pollutant characterized by its high toxicity, persistence and bioaccumulation within higher organisms. A thorough understanding of the microbial metabolic pathways involved in p-cresol degradation is critical for the design and optimization of microbial wastewater treatment systems. Despite numerous studies on the degradation pathways of p-cresol by various microbial species, the metabolic diversity within a single strain remains largely unexplored. This study investigated the metabolic mechanism of p-cresol in <em>Bacillus subtilis</em> ZW, a bacterium capable of degrading p-cresol. Through LC-MS analysis, we identified twelve distinct metabolic intermediates in the culture of strain ZW, leading to the proposal of three plausible degradation pathways. These include methyl hydroxylation, direct aromatic ring hydroxylation, and phosphorylation of the hydroxyl group, and all of which may concurrently contribute to p-cresol biodegradation by strain ZW. Further study showed that the genome of strain ZW harbored 47 coding genes associated with the degradation of p-cresol and its structural analogs, underscoring the metabolic versatility of this strain and its potential for xenobiotic biodegradation. These findings contribute valuable insights into the biodegradation mechanisms of pollutants. Under optimal degradation conditions of 35 °C and pH 7.0, strain ZW demonstrated the capacity to metabolize 27.5 % of p-cresol (10 mg/L) in minimal salt media within a week, and was capable of completely degrading 10 mg/L p-cresol in wastewater within five days. The potential utility of strain ZW in the bioremediation of p-cresol and other aromatic compounds is thus evident.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105933"},"PeriodicalIF":4.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-05DOI: 10.1016/j.ibiod.2024.105931
Jianzhao Li , Yangfan Xu , Longfei Li , Naidong Xiao , Mengjie Qu , Xiaoqiong Wan , Yumei Hua , Jianwei Zhao
Complete ammonia oxidizers (comammox bacteria) can convert ammonia into nitric acid through single-step nitrification. This study explored the spatial variations of comammox bacteria in the lakeshore area of the Houguan Lake in Wuhan, China. The abundance of the two comammox bacteria clades and two traditional ammonia-oxidizing microorganisms, ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), generally showed a gradually decreasing trend from the shore to the water. Moreover, a similar decreasing trend was observed for the respective and total nitrification rate of three types of ammonia-oxidizing microorganisms. The average nitrification rate of AOA, AOB and comammox bacteria was 0.568, 0.718, and 0.935 mg N kg−1 d−1, respectively. Besides, comammox bacteria exhibited a high biological diversity, with clade A and clade B and three subclades of clade A all present. Among different clades, clade B played a dominant role in the ammonia oxidation process. Both the abundance and nitrification rate of comammox bacteria were significantly positively correlated with total carbon and total nitrogen, indicating that these two nutrient substances are important factors influencing this microorganism. Our results demonstrate that the spatial variations of environmental elements in the lakeshore area lead to gradual decreases of comammox bacteria from the shore to the water.
完全氨氧化细菌（comammox bacteria）可通过单步硝化作用将氨转化为硝酸。本研究探讨了中国武汉后官湖湖岸地区氨氧化细菌的空间变化。研究结果表明，两种复合氧化细菌支系以及两种传统的氨氧化微生物--氨氧化古细菌（AOA）和氨氧化细菌（AOB）的丰度总体上呈现出从湖岸向水体逐渐降低的趋势。此外，三种氨氧化微生物的各自硝化率和总硝化率也呈类似的下降趋势。AOA 菌、AOB 菌和兼氧菌的平均硝化速率分别为 0.568、0.718 和 0.935 毫克 N kg-1 d-1。此外，复合氧化细菌表现出较高的生物多样性，A 支系、B 支系和 A 支系的三个亚支系均存在。在不同支系中，支系 B 在氨氧化过程中起主导作用。复合氧化细菌的丰度和硝化率均与总碳和总氮呈显著正相关，表明这两种营养物质是影响该微生物的重要因素。我们的研究结果表明，湖岸地区环境要素的空间变化导致彗星菌从湖岸向水中逐渐减少。
{"title":"Shore-to-water spatial variations of complete ammonia oxidizers in a lake in Wuhan, China","authors":"Jianzhao Li , Yangfan Xu , Longfei Li , Naidong Xiao , Mengjie Qu , Xiaoqiong Wan , Yumei Hua , Jianwei Zhao","doi":"10.1016/j.ibiod.2024.105931","DOIUrl":"10.1016/j.ibiod.2024.105931","url":null,"abstract":"<div><div>Complete ammonia oxidizers (comammox bacteria) can convert ammonia into nitric acid through single-step nitrification. This study explored the spatial variations of comammox bacteria in the lakeshore area of the Houguan Lake in Wuhan, China. The abundance of the two comammox bacteria clades and two traditional ammonia-oxidizing microorganisms, ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), generally showed a gradually decreasing trend from the shore to the water. Moreover, a similar decreasing trend was observed for the respective and total nitrification rate of three types of ammonia-oxidizing microorganisms. The average nitrification rate of AOA, AOB and comammox bacteria was 0.568, 0.718, and 0.935 mg N kg<sup>−1</sup> d<sup>−1</sup>, respectively. Besides, comammox bacteria exhibited a high biological diversity, with clade A and clade B and three subclades of clade A all present. Among different clades, clade B played a dominant role in the ammonia oxidation process. Both the abundance and nitrification rate of comammox bacteria were significantly positively correlated with total carbon and total nitrogen, indicating that these two nutrient substances are important factors influencing this microorganism. Our results demonstrate that the spatial variations of environmental elements in the lakeshore area lead to gradual decreases of comammox bacteria from the shore to the water.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105931"},"PeriodicalIF":4.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-05DOI: 10.1016/j.ibiod.2024.105937
Getnet Belay , Carolina Suarez , Addis Simachew , Catherine J. Paul
Alkaliphilic microorganisms are one option for the treatment of cyanide-polluted wastewater. This study reports the degradation of cyanide from simulated gold mine wastewater using alkaliphilic microbial consortia harvested from a soda lake, Lake Chitu, Ethiopia. A novel aerobic-anoxic integrated treatment setup was established for the treatment process. Colorimetry was used to measure residual cyanide concentration, and 16S rRNA amplicon gene sequencing was used to study microbial diversity. This treatment system was able to degrade 97.49 ± 0.12% of 200 mg/L sodium cyanide. However, changes were observed (p < 0.05) when the established consortia were stressed with heavy metals. About 28 % of the initial inoculum persisted until the end of the treatment days. Twenty-eight bacterial phyla were identified, with Firmicutes, Proteobacteria, and Bacteroidota being the most abundant. At the end of the treatment process, Alkalibacterium (74.43%), Exaguobacterium (6.6%), and Halomonas (3.89%) were dominant. These findings indicate that alkaliphilic microbial consortiums from Ethiopian Rift Valley soda lakes are effective for the treatment of cyanide-polluted wastewater.
{"title":"A systematic evaluation of alkaliphilic microbial consortia from a soda lake for the biodegradation of cyanide-rich wastewater","authors":"Getnet Belay , Carolina Suarez , Addis Simachew , Catherine J. Paul","doi":"10.1016/j.ibiod.2024.105937","DOIUrl":"10.1016/j.ibiod.2024.105937","url":null,"abstract":"<div><div>Alkaliphilic microorganisms are one option for the treatment of cyanide-polluted wastewater. This study reports the degradation of cyanide from simulated gold mine wastewater using alkaliphilic microbial consortia harvested from a soda lake, Lake Chitu, Ethiopia. A novel aerobic-anoxic integrated treatment setup was established for the treatment process. Colorimetry was used to measure residual cyanide concentration, and 16S rRNA amplicon gene sequencing was used to study microbial diversity. This treatment system was able to degrade 97.49 ± 0.12% of 200 mg/L sodium cyanide. However, changes were observed (p < 0.05) when the established consortia were stressed with heavy metals. About 28 % of the initial inoculum persisted until the end of the treatment days. Twenty-eight bacterial phyla were identified, with Firmicutes, Proteobacteria, and Bacteroidota being the most abundant. At the end of the treatment process, <em>Alkalibacterium</em> (74.43%), <em>Exaguobacterium</em> (6.6%), and <em>Halomonas</em> (3.89%) were dominant. These findings indicate that alkaliphilic microbial consortiums from Ethiopian Rift Valley soda lakes are effective for the treatment of cyanide-polluted wastewater.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105937"},"PeriodicalIF":4.1,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-02DOI: 10.1016/j.ibiod.2024.105935
Zheng-Fei Yan , Qing-Song Huang , Jing Yang , Xue-Yi Qiao , Bo Xu , Wei Xia , Ling-Qia Su
Microbial degradation is considered an effective and sustainable technique for the remediation of oily sludge; thus, the acquisition of crude oil-degrading bacteria is crucial for effective bioremediation. This study introduces a novel domestication-enrichment-isolation (DEI) strategy to isolate crude oil-degrading bacteria from oily sludge. Two strains, Rhodococcus rhodochrous JS-24 (R.rh) and Gordonia cholesterolivorans JS-13 (G.ch), demonstrated the highest degradation rates of 53.7% and 34.6%, respectively, within 7 days. While no synergistic effect was observed with their combined use in free bacterial consortia, and the overall degradation rate decreased to 51.9 %, which was weaker than that of R. rh treatment alone. The decrease in degradation rate is attributed to microbial flocculation towards crude oil: most droplets of crude oil were encapsulated into spherical aggregations by G. ch, thereby hindering the contact and degradation of droplets by R. rh. In contrast, immobilization technology significantly enhanced crude oil degradation by eliminating this flocculation effect. The immobilized bacterial consortium achieved a 95.5% degradation rate, representing the highest degradation rate reported for bacterial consortia. This study reveals for the first time that the side effects of bioflocculation on crude oil degradation and provides guidance for the construction of bacterial consortium.
微生物降解被认为是一种有效且可持续的油性污泥修复技术；因此，获得原油降解细菌对于有效的生物修复至关重要。本研究介绍了一种新型的驯化-富集-分离（DEI）策略，用于从含油污泥中分离原油降解细菌。两株菌株，即 Rhodococcus rhodochrous JS-24 (R.rh) 和 Gordonia cholesterolivorans JS-13 (G.ch)，在 7 天内分别表现出 53.7% 和 34.6% 的最高降解率。但在游离细菌群中联合使用这两种细菌时，没有观察到协同效应，总体降解率降至 51.9%，比单独使用 R. rh 的降解率低。降解率下降的原因是微生物对原油的絮凝作用：大多数原油液滴被 G. ch 包裹成球形聚集体，从而阻碍了 R. rh 与液滴的接触和降解。相比之下，固定化技术消除了这种絮凝效应，从而大大提高了原油降解效果。固定化细菌联合体的降解率达到了 95.5%，是目前报道的细菌联合体中降解率最高的。这项研究首次揭示了生物絮凝对原油降解的副作用，为构建细菌联合体提供了指导。
{"title":"Enhanced degradation of crude oil by immobilized bacterial consortium through eliminating microbial flocculation towards crude oil","authors":"Zheng-Fei Yan , Qing-Song Huang , Jing Yang , Xue-Yi Qiao , Bo Xu , Wei Xia , Ling-Qia Su","doi":"10.1016/j.ibiod.2024.105935","DOIUrl":"10.1016/j.ibiod.2024.105935","url":null,"abstract":"<div><div>Microbial degradation is considered an effective and sustainable technique for the remediation of oily sludge; thus, the acquisition of crude oil-degrading bacteria is crucial for effective bioremediation. This study introduces a novel domestication-enrichment-isolation (DEI) strategy to isolate crude oil-degrading bacteria from oily sludge. Two strains, <em>Rhodococcus rhodochrous</em> JS-24 (R.rh) and <em>Gordonia cholesterolivorans</em> JS-13 (G.ch), demonstrated the highest degradation rates of 53.7% and 34.6%, respectively, within 7 days. While no synergistic effect was observed with their combined use in free bacterial consortia, and the overall degradation rate decreased to 51.9 %, which was weaker than that of R. rh treatment alone. The decrease in degradation rate is attributed to microbial flocculation towards crude oil: most droplets of crude oil were encapsulated into spherical aggregations by G. ch, thereby hindering the contact and degradation of droplets by R. rh. In contrast, immobilization technology significantly enhanced crude oil degradation by eliminating this flocculation effect. The immobilized bacterial consortium achieved a 95.5% degradation rate, representing the highest degradation rate reported for bacterial consortia. This study reveals for the first time that the side effects of bioflocculation on crude oil degradation and provides guidance for the construction of bacterial consortium.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105935"},"PeriodicalIF":4.1,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-27DOI: 10.1016/j.ibiod.2024.105930
Qiangqiang Xia, Lisha Shi, Ding Ma, Tao Xu
To study the degradation behaviors of asphalt by microorganisms and reveal biodegradation mechanism of asphalt, 16S rRNA full-length sequencing was utilized to analyze the collected samples from three forest areas. Five strains, namely Sphingomonas polyaromaticivorans, Pseudomonas putida, Pseudomonas fluorescens, Bacillus cereus, and Bacillus subtilis, were selected for compounding microbial consortium to study its degradation effects on asphalt. The micromorphology, elemental chemical states, micromechanical properties, molecular weight distribution, and functional groups of degraded asphalt were studied using environmental scanning electron microscopy, X-ray photoelectron spectroscopy, atomic force microscopy, gel permeation chromatography, and Fourier transform infrared spectroscopy. The results show that the biodegradation of asphalt by microbial consortium increases the surface roughness of asphalt and reduces surface adhesion. Asphalt is used as the sole carbon source by microbial consortium, resulting in a continuous decrease in the contents of C and N elements, while the content of O element is continuously increased. Simultaneously, under the action of microbial enzymes, C-C/C-H bonds which are combined with molecular oxygen in asphalt are continuously broken and converted to C-O-C/C-O-H bonds. The continuous reductions in methylene index and butadiene index of asphalt, as well as the production of carbonyl functional groups confirm the oxidative degradation of asphalt by microbial consortium. Additionally, with the extension of degradation time, the large molecular substances in asphalt are gradually degraded by microbial consortium, leading to a more concentrated molecular weight distribution. This study is of great importance to understand biodegradation mechanism of asphalt.
{"title":"Biodegradation mechanism of asphalt by microbial consortia in asphalt pavement of forest area road","authors":"Qiangqiang Xia, Lisha Shi, Ding Ma, Tao Xu","doi":"10.1016/j.ibiod.2024.105930","DOIUrl":"10.1016/j.ibiod.2024.105930","url":null,"abstract":"<div><div>To study the degradation behaviors of asphalt by microorganisms and reveal biodegradation mechanism of asphalt, 16S rRNA full-length sequencing was utilized to analyze the collected samples from three forest areas. Five strains, namely <em>Sphingomonas polyaromaticivorans</em>, <em>Pseudomonas putida</em>, <em>Pseudomonas fluorescens</em>, <em>Bacillus cereus</em>, and <em>Bacillus subtilis</em>, were selected for compounding microbial consortium to study its degradation effects on asphalt. The micromorphology, elemental chemical states, micromechanical properties, molecular weight distribution, and functional groups of degraded asphalt were studied using environmental scanning electron microscopy, X-ray photoelectron spectroscopy, atomic force microscopy, gel permeation chromatography, and Fourier transform infrared spectroscopy. The results show that the biodegradation of asphalt by microbial consortium increases the surface roughness of asphalt and reduces surface adhesion. Asphalt is used as the sole carbon source by microbial consortium, resulting in a continuous decrease in the contents of C and N elements, while the content of O element is continuously increased. Simultaneously, under the action of microbial enzymes, C-C/C-H bonds which are combined with molecular oxygen in asphalt are continuously broken and converted to C-O-C/C-O-H bonds. The continuous reductions in methylene index and butadiene index of asphalt, as well as the production of carbonyl functional groups confirm the oxidative degradation of asphalt by microbial consortium. Additionally, with the extension of degradation time, the large molecular substances in asphalt are gradually degraded by microbial consortium, leading to a more concentrated molecular weight distribution. This study is of great importance to understand biodegradation mechanism of asphalt.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105930"},"PeriodicalIF":4.1,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-26DOI: 10.1016/j.ibiod.2024.105926
Binbin Ran , Qiang An , Shuman Deng , Jiali Song , Zhiruo Huang , Bin Zhao
With the continuous development of electroplating industry, a large amount of electroplating wastewater is generated, which can be harmful to soil and basement. Introducing microorganisms into polluted soil can improve the soil environment, but it has the disadvantages of easy loss and low activity. In this study, the synergistic effect of biochar and strain L1 (BL1) was utilized to effectively reduce the risk of groundwater contamination by Ni(II), Cu(II), Cr(VI), and Zn(II), which are common heavy metals in electroplating wastewater. And the mechanism was found as BL1 was found to increase the porosity and water retention of the soil by specific surface area determination (BET) and scanning electron microscopy (SEM), favored the growth of soil microorganisms. It was found that BL1 could improve soil pH, enzyme activity, total organic carbon and other indicators by measuring soil physical and chemical properties. The results of microbial community analysis showed that BL1 increased the diversity of soil community and enriched microorganisms with nitrification and denitrification functions, thus promoting the removal of NH4+-N and NO3-N. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) diffraction results showed that -OH, -CH, C=O, Si-O-Si, CO32− and PO43− of BL1 complexed with heavy metals to form precipitates. Thus, the conversion of heavy metals to the stable state was promoted. These results show that the addition of BL1 can effectively improve the soil environment and promote the self-recovery of soil function.
{"title":"Synergistic remediation of electroplating wastewater contaminated soil and reduction of risk of groundwater contamination by biochar and Pseudomonas hibiscicola strain L1","authors":"Binbin Ran , Qiang An , Shuman Deng , Jiali Song , Zhiruo Huang , Bin Zhao","doi":"10.1016/j.ibiod.2024.105926","DOIUrl":"10.1016/j.ibiod.2024.105926","url":null,"abstract":"<div><div>With the continuous development of electroplating industry, a large amount of electroplating wastewater is generated, which can be harmful to soil and basement. Introducing microorganisms into polluted soil can improve the soil environment, but it has the disadvantages of easy loss and low activity. In this study, the synergistic effect of biochar and strain L1 (BL1) was utilized to effectively reduce the risk of groundwater contamination by Ni(II), Cu(II), Cr(VI), and Zn(II), which are common heavy metals in electroplating wastewater. And the mechanism was found as BL1 was found to increase the porosity and water retention of the soil by specific surface area determination (BET) and scanning electron microscopy (SEM), favored the growth of soil microorganisms. It was found that BL1 could improve soil pH, enzyme activity, total organic carbon and other indicators by measuring soil physical and chemical properties. The results of microbial community analysis showed that BL1 increased the diversity of soil community and enriched microorganisms with nitrification and denitrification functions, thus promoting the removal of NH<sub>4</sub><sup>+</sup>-N and NO<sub>3</sub>-N. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) diffraction results showed that -OH, -CH, C=O, Si-O-Si, CO<sub>3</sub><sup>2−</sup> and PO<sub>4</sub><sup>3−</sup> of BL1 complexed with heavy metals to form precipitates. Thus, the conversion of heavy metals to the stable state was promoted. These results show that the addition of BL1 can effectively improve the soil environment and promote the self-recovery of soil function.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105926"},"PeriodicalIF":4.1,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-26DOI: 10.1016/j.ibiod.2024.105928
Adam Krajewski, Andrzej Antczak, Dominika Szadkowska, Piotr Witomski
Hylotrupes bajulus L. is a dangerous destroyer of wooden structures. Monoterpenes (mainly α-pinene and 3-carene) attract of this beetle females to lay eggs on wood. The phenomenon of sporadic occurrence of old house borer larvae in old wooden structures and its reappearance in these structures after the previous use of hot air to old house borer combating has been known for many decades. The causes of these phenomena were not explained. In this work, it was hypothesized that the cause of these phenomena was poor attraction of this wood for females due to the reduced amount of monoterpenes in such wood. An experiment was carried out on the possibility of developing old house borer larvae in old natural aged wood. Tests were also carried out on the profile of monoterpenes in uninfested old natural aged wood, contemporary wood and contemporary wood after heating at high temperature. The possibility of development of newly hatched H. bajulus larvae in old natural aged wood and a high concentration of monoterpenes (mainly α-pinene and 3-carene) in such wood were found. The sporadic presence of old house borer larvae in very old wooden structures cannot be explained directly by a reduction in the concentration of attracting monoterpenes. The concentration of monoterpenes in contemporary wood after exposure to high temperature was whereas significantly reduced. This may be the reason for sporadic cases of repeated attacks on structures previously exposed to very hot air.
Hylotrupes bajulus L. 是木质结构的危险破坏者。单萜烯（主要是 α-蒎烯和 3-蒈烯）会吸引这种甲虫的雌虫在木材上产卵。旧木结构中零星出现老房蛀虫幼虫，以及以前使用热空气防治老房蛀虫后这些结构中再次出现老房蛀虫幼虫的现象已为人所知几十年。但造成这些现象的原因却没有得到解释。在这项工作中，我们假设造成这些现象的原因是这种木材对雌虫的吸引力差，因为这种木材中的单萜含量减少。我们进行了一项实验，研究老房子螟幼虫在天然老化木材中生长的可能性。此外，还对未受侵染的天然老龄木材、现代木材和高温加热后的现代木材中的单萜烯含量进行了测试。结果发现，新孵化的巴氏蝙蝠幼虫有可能在天然老龄木材中生长，而且这些木材中的单萜烯（主要是 α-蒎烯和 3-蒈烯）浓度较高。在年代久远的木质结构中零星出现老屋蛀虫幼虫，并不能直接解释为吸引幼虫的单萜烯浓度降低了。暴露于高温后，当代木材中单萜烯的浓度明显降低。这可能是以前暴露在高温空气中的木结构反复受到攻击的零星案例的原因。
{"title":"Monoterpenes profile and high temperature as a potential factors in the infestation of wooden structures by Hylotrupes bajulus L","authors":"Adam Krajewski, Andrzej Antczak, Dominika Szadkowska, Piotr Witomski","doi":"10.1016/j.ibiod.2024.105928","DOIUrl":"10.1016/j.ibiod.2024.105928","url":null,"abstract":"<div><div><em>Hylotrupes bajulus</em> L. is a dangerous destroyer of wooden structures. Monoterpenes (mainly α-pinene and 3-carene) attract of this beetle females to lay eggs on wood. The phenomenon of sporadic occurrence of old house borer larvae in old wooden structures and its reappearance in these structures after the previous use of hot air to old house borer combating has been known for many decades. The causes of these phenomena were not explained. In this work, it was hypothesized that the cause of these phenomena was poor attraction of this wood for females due to the reduced amount of monoterpenes in such wood. An experiment was carried out on the possibility of developing old house borer larvae in old natural aged wood. Tests were also carried out on the profile of monoterpenes in uninfested old natural aged wood, contemporary wood and contemporary wood after heating at high temperature. The possibility of development of newly hatched <em>H. bajulus</em> larvae in old natural aged wood and a high concentration of monoterpenes (mainly α-pinene and 3-carene) in such wood were found. The sporadic presence of old house borer larvae in very old wooden structures cannot be explained directly by a reduction in the concentration of attracting monoterpenes. The concentration of monoterpenes in contemporary wood after exposure to high temperature was whereas significantly reduced. This may be the reason for sporadic cases of repeated attacks on structures previously exposed to very hot air.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105928"},"PeriodicalIF":4.1,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142318989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-26DOI: 10.1016/j.ibiod.2024.105929
Ruzhen Liu , Zhichao Wang , Minghuang Ling , Kai Zhang , Wei Xu , Zhijie Xu , Xiaomei Huang , Ying Qiao , Yu Luo , Wenzhou Zhang , Hans-Peter Grossart , Zhuhua Luo
Plastic pollution has emerged as a significant environmental issue in marine ecosystems. The prolonged presence of plastics in mangrove sediments can have a substantial impact on the carbon and nitrogen cycles of these vital environments. We conducted in situ burial exposure experiments to assess microbial colonization and activity on biodegradable and refractory plastics in mangrove sediments, with a focus on their influence on nitrogen cycling. Variations in microbial community succession and metabolic processes on plastic surfaces were primarily determined by the type of plastic polymer. After 60 days of exposure, the abundance of denitrifying bacteria and denitrification-related genes (narG, nirS, nosZ, and norB) was higher on biodegradable plastics. After 360 days, however, a high abundance of ammonia-oxidizing archaea and nitrification-related genes (pmoA-amoA, pmoB-amoB, pmoC-amoC, and hao) were found on refractory plastics. Consequently, plastic pollution in mangrove sediments could disrupt the nitrogen cycling equilibrium in these ecosystems, underscoring the critical necessity to regulate and alleviate the detrimental impacts of plastic pollution in crucial coastal areas.
{"title":"Dynamics of plastisphere microbial communities in mangrove sediments and their potential impact on N-cycling","authors":"Ruzhen Liu , Zhichao Wang , Minghuang Ling , Kai Zhang , Wei Xu , Zhijie Xu , Xiaomei Huang , Ying Qiao , Yu Luo , Wenzhou Zhang , Hans-Peter Grossart , Zhuhua Luo","doi":"10.1016/j.ibiod.2024.105929","DOIUrl":"10.1016/j.ibiod.2024.105929","url":null,"abstract":"<div><div>Plastic pollution has emerged as a significant environmental issue in marine ecosystems. The prolonged presence of plastics in mangrove sediments can have a substantial impact on the carbon and nitrogen cycles of these vital environments. We conducted <em>in situ</em> burial exposure experiments to assess microbial colonization and activity on biodegradable and refractory plastics in mangrove sediments, with a focus on their influence on nitrogen cycling. Variations in microbial community succession and metabolic processes on plastic surfaces were primarily determined by the type of plastic polymer. After 60 days of exposure, the abundance of denitrifying bacteria and denitrification-related genes (<em>narG</em>, <em>nirS</em>, <em>nosZ</em>, and <em>norB</em>) was higher on biodegradable plastics. After 360 days, however, a high abundance of ammonia-oxidizing archaea and nitrification-related genes (<em>pmoA-amoA</em>, <em>pmoB-amoB</em>, <em>pmoC-amoC</em>, and <em>hao</em>) were found on refractory plastics. Consequently, plastic pollution in mangrove sediments could disrupt the nitrogen cycling equilibrium in these ecosystems, underscoring the critical necessity to regulate and alleviate the detrimental impacts of plastic pollution in crucial coastal areas.</div></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105929"},"PeriodicalIF":4.1,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-09-21DOI: 10.1016/j.ibiod.2024.105924
Himanshi Gupta , Prithvi Pal Singh , S.G. Eswara Reddy
Essential oil (EO) of Citrus limon fresh (LF) and lemon waste peel (LW) was extracted using the hydro distillation method. A total of 17 chemical constituents (97.02–97.26%) were identified using GC, GC-MS, and NMR techniques. Major monoterpene hydrocarbons including d-limonene (52.42–54.17%), α-terpineol (16.82–21.15%), β-pinene (6.74–9.15%) and γ-terpinene (2.16–3.59%) were further identified using 1H and 13C NMR analysis. Furthermore, extracted EOs, their synergistic combinations and major constituent (d-limonene) were evaluated for fumigant toxicity, repellence, and ovipositional inhibitory (OI) potential using without food and with food conditions against pulse beetle, Callosobruchus chinensis and Callosobruchus maculatus. d-limonene was found to be most effective against C. chinensis and C. maculatus followed by LW oil in all the evaluated assays. EO of LW was found more effective against C. chinensis (LC50 = 2899.11 μl L−1) without food after 96 h than lemon fresh. Among synergistic combinations, LW and LF at 3:1 ratio without food reported more effective to C. chinensis (LC50 = 277.85 μl L−1) and C. maculatus (LC50 = 322.38 μl L−1) without food after 96 h. In repellent assay, EO of LW also displayed higher repellent to both species (RC50 = 430.71 to 525.56 and μl L−1). EO of LW showed higher OI (50.14 ± 3.09%) against C. chinensis at higher concentration after 24 h. EOs of LF and LW also inhibited glutathione -S-transferase and acetylcholine esterase activity in C. chinensis and C. maculatus.
{"title":"Exploring the chemical profiling and insecticidal properties of essential oils from fresh and discarded lemon peels, Citrus limon against pulse beetle","authors":"Himanshi Gupta , Prithvi Pal Singh , S.G. Eswara Reddy","doi":"10.1016/j.ibiod.2024.105924","DOIUrl":"10.1016/j.ibiod.2024.105924","url":null,"abstract":"<div><p>Essential oil (EO) of <em>Citrus limon</em> fresh (LF) and lemon waste peel (LW) was extracted using the hydro distillation method. A total of 17 chemical constituents (97.02–97.26%) were identified using GC, GC-MS, and NMR techniques. Major monoterpene hydrocarbons including d-limonene (52.42–54.17%), <em>α</em>-terpineol (16.82–21.15%), <em>β</em>-pinene (6.74–9.15%) and <em>γ</em>-terpinene (2.16–3.59%) were further identified using <sup>1</sup>H and <sup>13</sup>C NMR analysis. Furthermore, extracted EOs, their synergistic combinations and major constituent (d-limonene) were evaluated for fumigant toxicity, repellence, and ovipositional inhibitory (OI) potential using without food and with food conditions against pulse beetle, <em>Callosobruchus chinensis</em> and <em>Callosobruchus maculatus.</em> d-limonene was found to be most effective against <em>C. chinensis</em> and <em>C. maculatus</em> followed by LW oil in all the evaluated assays. EO of LW was found more effective against <em>C. chinensis</em> (LC<sub>50</sub> = 2899.11 μl L<sup>−1</sup>) without food after 96 h than lemon fresh. Among synergistic combinations, LW and LF at 3:1 ratio without food reported more effective to <em>C. chinensis</em> (LC<sub>50</sub> = 277.85 μl L<sup>−1</sup>) and <em>C. maculatus</em> (LC<sub>50</sub> = 322.38 μl L<sup>−1</sup>) without food after 96 h. In repellent assay, EO of LW also displayed higher repellent to both species (RC<sub>50</sub> = 430.71 to 525.56 and μl L<sup>−1</sup>). EO of LW showed higher OI (50.14 ± 3.09%) against <em>C. chinensis</em> at higher concentration after 24 h. EOs of LF and LW also inhibited glutathione -S-transferase and acetylcholine esterase activity in <em>C. chinensis</em> and <em>C. maculatus.</em></p></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"196 ","pages":"Article 105924"},"PeriodicalIF":4.1,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0964830524001951/pdfft?md5=39d31776ef419da1b48350dfda38dd6f&pid=1-s2.0-S0964830524001951-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142274853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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