International Biodeterioration & Biodegradation最新文献_第4页

Performance, sulfate removal, and microbial response of anaerobic baffled reactors coupled with microbial electrochemical cells treating lithium battery wastewater containing N-methylpyrrolidone 厌氧折流板反应器耦合微生物电化学电池处理含n -甲基吡咯烷酮锂电池废水的性能、硫酸盐去除及微生物响应

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-19 DOI: 10.1016/j.ibiod.2025.106251

Zhengpeng Chen , Jieying Liu , Tingting Huang , Shu Gao , Tongshu Dong , Jiahao Chen , Yuxiang Lu , Shenglong Chen , Chengyuan Su

N-methylpyrrolidone (NMP) is widely used as an effective solvent for various lithium battery cathode materials. In this study, a microbial electrolysis cell-anaerobic baffled reactor (MEC-ABR) was developed to investigate the performance of simultaneous removal of NMP and sulfate under varying NMP concentrations. The MEC-ABR system achieved an average sulfate removal of 88.13 %, significantly higher than the ABR control (78.64 %), and removal progressively increased across stages, indicating enhanced sulfate reduction via microbial electron transfer. In the first stage (NMP 1 mmol/L) and third stage (NMP 10 mmol/L), the removal rates of NMP by the MEC-ABR were about 20 % and 88 %, respectively. The NMP removal improved over time (MEC-ABR 88.96 %, ABR 90.73 %), but MEC did not significantly enhance NMP removal, attributed to oxygen-dependent NMP degradation and electron competition with methanogens and sulfate-reducing bacteria. Fluorescence 3D excitation-emission matrix spectroscopy (EEM), sludge morphology, cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) analyses confirmed that MEC promoted secretion of electron-active proteins, enhanced electron generation and transfer, and improved sludge flocculation. Microbial and functional gene analyses revealed dominant methanogens (Methanothrix) and sulfate-reducing bacteria, increased expression of electron-generation (mdh) and electron-transfer (sdhA) genes, and voltage-stimulated amo/hao expression, facilitating NMP hydrolysis and nitrification. Overall, MEC enhanced system stability and sulfate reduction, while NMP removal remained limited by oxygen-dependent degradation and microbial electron competition. These findings provide mechanistic insights into MEC-ABR operation and offer guidance for optimizing lithium battery wastewater treatment.

n -甲基吡咯烷酮（NMP）被广泛用作各种锂电池正极材料的有效溶剂。在本研究中，开发了一种微生物电解-厌氧折流板反应器（MEC-ABR），研究了在不同NMP浓度下同时去除NMP和硫酸盐的性能。MEC-ABR系统的硫酸盐平均去除率为88.13%，显著高于ABR对照（78.64%），并且在各个阶段的去除率逐渐增加，表明微生物电子转移增强了硫酸盐的还原。在第一阶段（NMP为1 mmol/L）和第三阶段（NMP为10 mmol/L）， MEC-ABR对NMP的去除率分别为20%和88%。随着时间的推移，NMP的去除率有所提高（MEC-ABR为88.96%，ABR为90.73%），但MEC对NMP的去除率没有显著提高，原因是NMP的氧依赖性降解以及与产甲烷菌和硫酸盐还原菌的电子竞争。荧光3D激发-发射矩阵光谱（EEM）、污泥形态、循环伏安法和电化学阻抗谱（EIS）分析证实，MEC促进了电子活性蛋白的分泌，增强了电子的产生和转移，改善了污泥的絮凝。微生物和功能基因分析显示，优势产甲烷菌（Methanothrix）和硫酸盐还原菌，电子产生（mdh）和电子转移（sdhA）基因的表达增加，以及电压刺激的amo/hao表达，促进了NMP的水解和硝化。总体而言，MEC增强了系统稳定性和硫酸盐还原，而NMP的去除仍然受到氧依赖降解和微生物电子竞争的限制。这些发现为MEC-ABR操作提供了机理见解，并为优化锂电池废水处理提供了指导。

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引用次数: 0

Triclosan-induced shifts in aryl sulfatase activity: Implications in aquatic sediments 三氯生诱导芳基硫酸盐酶活性的变化：对水生沉积物的影响

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-17 DOI: 10.1016/j.ibiod.2025.106247

Priya Varghese , Kundan Kumar , Saurav Kumar , S.P. Shukla , Sutanu Karmakar , Pritam Sarkar , Puja Rani Basak

The effects of triclosan (TCS), a widely distributed broad-spectrum antimicrobial agent, on aryl sulfatase (ARS) activity in aquatic sediments were systematically investigated. Sediment samples from Versova Creek, Mumbai, were exposed to TCS concentrations of 0, 3, and 6 mg/kg over 28 days in a microcosm set-up. ARS activity increased in a concentration-dependent manner, rising by 37 % at 3 mg/kg and 20 % at 6 mg/kg on the final day (51.66 ± 1.36 and 45.18 ± 0.15 PNP g/hr, respectively) relative to the initial control value (37.65 ± 0.64 PNP g/hr). This activity was positively correlated with sulfur availability, demonstrating enhanced sulfur cycling under TCS exposure. Metagenomic analysis identified Pseudomonas, Corynebacterium, and Aeromonas species as dominant ARS producers, with microbial diversity impacted by TCS concentration. At lower concentrations, TCS stimulated ARS-producing bacteria, which may have implications for bioremediation, while higher concentrations inhibited heterotrophic bacterial growth. The findings highlighted the role of TCS in altering sediment microbial dynamics and biogeochemistry, indicating its potential impact on sediment nutrient cycles and microbial functions essential for ecosystem stability. These alterations underscore the importance of further research into pollutant-driven shifts in microbial and nutrient cycling, which could contribute towards the development of sustainable environmental management strategies, including effective bioremediation approaches.

系统研究了广泛分布的广谱抗菌药物三氯生（TCS）对水生沉积物中芳基硫酸酯酶（ARS）活性的影响。来自孟买Versova Creek的沉积物样本在微观环境中暴露于浓度为0、3和6 mg/kg的TCS中28天。ARS活性呈浓度依赖性增加，与初始对照值（37.65±0.64 PNP g/hr）相比，3 mg/kg组和6 mg/kg组在最后一天分别提高了37%和20%（分别为51.66±1.36和45.18±0.15 PNP g/hr）。该活性与硫有效性正相关，表明在TCS暴露下硫循环增强。宏基因组分析发现假单胞菌、杆状杆菌和气单胞菌是ARS的主要产生菌，其微生物多样性受TCS浓度的影响。在较低浓度下，TCS刺激产生ars的细菌，这可能对生物修复有影响，而较高浓度则抑制异养细菌的生长。这些发现强调了TCS在改变沉积物微生物动力学和生物地球化学中的作用，表明其对生态系统稳定所必需的沉积物养分循环和微生物功能的潜在影响。这些变化强调了进一步研究污染物驱动的微生物和养分循环变化的重要性，这可能有助于制定可持续的环境管理战略，包括有效的生物修复方法。

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引用次数: 0

Degradation of polystyrene microplastics mediated by intestinal microorganisms of earthworms and exploration of functional strain resources 蚯蚓肠道微生物对聚苯乙烯微塑料的降解及功能菌株资源的探索

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-15 DOI: 10.1016/j.ibiod.2025.106249

Liping Zhang , Meitong Li , Shaolin Qiu , Xuening Fei , Lingyun Cao , Yuhong Xie , Xiaoqin Jin

PS, one of the most extensively used synthetic polymers, persists in terrestrial ecosystems owing to its high chemical stability and inherent resistance to degradation. Yet, the biological mechanisms for its degradation remain unclear. This study investigated the degradation of PS-MPs mediated by intestinal microorganisms from two earthworm species, Eisenia fetida and Perionyx guillelmi. An integrative approach combining SEM, GC-MS, and microbial diversity analysis was applied to characterize alterations, identify degradation intermediates, and assess gut microbial dynamics. The PS-MP content in soil decreased progressively, achieving degradation efficiencies of 2.33 % on day 7 and 56.67 % on day 28. Three bacterial strains, Bacillus cereus, Klebsiella variicola, and Citrobacter portucalensis, exhibited PS degradation efficiencies of 2.33 %, 2.48 %, and 2.60 %, respectively. Collectively, our findings provide compelling evidence that the earthworm intestinal microbiota actively mediate the biodegradation of PS-MPs. These results elucidate a microbial biodegradation pathway and highlight earthworm microorganisms as promising biocatalysts for sustainable plastic bioremediation.

PS是使用最广泛的合成聚合物之一，由于其高度的化学稳定性和固有的抗降解性而持续存在于陆地生态系统中。然而，其降解的生物学机制尚不清楚。本研究研究了两种蚯蚓肠道微生物对PS-MPs的降解作用，分别为臭Eisenia fetida和Perionyx guillelmi。采用SEM， GC-MS和微生物多样性分析相结合的综合方法来表征变化，识别降解中间体，并评估肠道微生物动力学。土壤中PS-MP含量逐渐降低，第7天和第28天降解效率分别为2.33%和56.67%。蜡样芽孢杆菌、水痘克雷伯菌和葡萄牙柠檬酸杆菌对PS的降解效率分别为2.33%、2.48%和2.60%。总的来说，我们的研究结果提供了令人信服的证据，表明蚯蚓肠道微生物群积极地介导PS-MPs的生物降解。这些结果阐明了微生物生物降解途径，并突出了蚯蚓微生物作为可持续塑料生物修复的有前途的生物催化剂。

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引用次数: 0

Antifungal activity of essential oils and their use as alternative wood preservatives against rot fungi 精油的抗真菌活性及其作为木材防腐剂的应用

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-15 DOI: 10.1016/j.ibiod.2025.106250

Tarcisio Francisco de Camargo , Adriana Terumi Itako , Cristian Soldi , Magnos Alan Vivian , Juliana Aparecida Teixeira Stanck Bireahls , Camila Alves Corrêa , João Batista Tolentino Júnior , Alexsandro Bayestorff da Cunha

The objective of this study was to verify the antifungal activity of three essential oils and their efficiency as natural wood preservatives against the action of the rot fungi Trametes versicolor, Pycnoporus sanguineus, and Gloeophyllum trabeum. The essential oils, extracted by hydrodistillation in a Clevenger apparatus, were obtained from leaves of the plants Cymbopogon citratus, Cupressus lusitanica, and Drimys brasiliensis, collected in the region of Curitibanos, Santa Catarina, Brazil. The chemical composition of the essential oils, their in vitro antifungal activity, and subsequent preservative treatment of Pinus taeda test specimens were determined. Chemical analysis indicated a higher percentage of isobornyl acetate (22.8 %), bicyclogermacrene (16.0 %), and α-citral (44.9 %) in the essential oils of C. lusitanica, D. brasiliensis, and C. citratus, respectively. The results indicate that all essential oils have in vitro antifungal potential against the tested decay fungi. Preservative treatment with C. citratus essential oil provided greater protection to the wood, which was classified, after treatment, as highly resistant, with less mass loss caused by the white rot fungus T. versicolor.

本研究的目的是验证三种精油的抗真菌活性，以及它们作为天然木材防腐剂对腐真菌Trametes versicolor， Pycnoporus sanguineus和Gloeophyllum trabeum的作用。精油在Clevenger装置中通过加氢蒸馏提取，从植物Cymbopogon citratus， Cupressus lusitanica和Drimys brasiliensis的叶子中获得，这些植物采集于巴西圣卡塔琳娜州库里提巴诺斯地区。测定了油松精油的化学成分、体外抗真菌活性以及随后的防腐处理。化学分析结果表明，芦西花、巴西花和柑桔精油中乙酸异硼酯（22.8%）、双环革烯（16.0%）和α-柠檬醛（44.9%）含量较高。结果表明，各挥发油对所测腐真菌均有体外抑菌作用。柑橘精油防腐处理对木材有更好的保护作用，处理后的木材被分类为高抗，由白腐真菌T. versicolor造成的质量损失较小。

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引用次数: 0

Penicillin and streptomycin in ethanol mist against spore-forming Bacillus bacteria isolated from surfaces of historical objects 乙醇雾中青霉素和链霉素对从历史文物表面分离的芽孢杆菌的抑制作用

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-13 DOI: 10.1016/j.ibiod.2025.106246

Anna Wawrzyk , Natalia Pydyn , Dorota Rybitwa , Nel Jastrzębiowska , Lilianna Szyk-Warszyńska , Małgorzata Zimowska , Jacek Gurgul , Dagmara Zeljaś , Filip Bielec

The objective of the study was to investigate the antimicrobial efficacy of ethanol mist enriched with penicillin and/or streptomycin and to examine its effects on the surface properties of model and historical textile materials from the collections of the Auschwitz-Birkenau State Museum (ABSM) in Oświęcim (Poland). Bacillus bacteria, which inhabited historical textile objects in the ABSM, were inoculated onto samples of textiles. Then, penicillin and/or streptomycin suspended in water or ethanol were applied in the form of mist. Sensitivity of the bacterial strains to the antibiotics was tested with disk diffusion (vegetative forms) and agar imprint (spores) methods. After that, surface alterations were analysed using SEM, confocal microscopy and XPS techniques. Even though initial effectiveness of presented disinfection method was observed, both for cells and spores, it resulted only in a temporary inhibition of the growth of tested bacteria. Importantly, subsequent analyses revealed that this treatment did not induce any detectable alterations in the surface morphology or chemistry of the textile materials. The developed method of applying antibiotics together with ethanol mist to increase effectiveness of ethanol against spore-forming bacteria is non-destructive and preserves the original structural and chemical integrity of historical fabric. However, the method has a biostatic effect on spore-forming Bacillus, not biocidal, so the addition of tested antibiotics does not allow the desired effect to be achieved. Nevertheless, ethanol in the form of mist without additives is biocidally effective against a wide range of microorganisms.

本研究的目的是研究含有青霉素和/或链霉素的乙醇薄雾的抗菌效果，并检查其对Oświęcim（波兰）奥斯威辛-比克瑙国家博物馆（ABSM）收藏的模型和历史纺织品表面性能的影响。将居住在ABSM历史纺织品上的芽孢杆菌接种到纺织品样品上。然后，将盘尼西林和/或链霉素悬浮在水或乙醇中以雾状施用。采用盘片扩散法（营养形态法）和琼脂印记法（孢子法）检测菌株对抗生素的敏感性。之后，使用扫描电镜、共聚焦显微镜和XPS技术分析表面变化。尽管观察到所提出的消毒方法对细胞和孢子的初步有效性，但它只能暂时抑制被测细菌的生长。重要的是，随后的分析表明，这种处理不会引起纺织材料表面形态或化学的任何可检测的变化。所开发的将抗生素与乙醇雾一起施用以提高乙醇对孢子形成细菌的有效性的方法是非破坏性的，并且保留了历史织物的原始结构和化学完整性。然而，该方法对孢子形成芽孢杆菌具有生物抑制作用，而不是杀菌剂，因此添加所测试的抗生素不能达到预期的效果。然而，不含添加剂的雾状乙醇对多种微生物具有杀灭效果。

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引用次数: 0

Long-term nitrogen removal through sludge augmentation in biochar-based constructed wetlands treating low C/N ratio wastewater 生物炭基人工湿地处理低碳氮比废水的污泥强化长期脱氮研究

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-11 DOI: 10.1016/j.ibiod.2025.106244

Deepti Negi , Punyasloke Bhadury , Achlesh Daverey

Efficient nitrogen (N) removal from low carbon-to-nitrogen (C/N) ratio wastewater remains a major challenge. This study evaluates the effect of sludge amendment [anaerobic (AnS) or activated sludge (AS)] on the long-term (227 days) performance of biochar-integrated vertical flow constructed wetlands (VFCWs) for the treatment of simulated low COD/N ratio (3.6 and 2) wastewater. TN removal efficiencies ranged from 30.59 to 94.97% in Phase I and 50.62–90.97% in Phase II, with the maximum removal observed in AS-inoculated CWs. Sludge addition enhanced N removal, with 26.77–50.96% of N remaining unaccounted, likely reflecting microbial transformations, gaseous emissions and other N losses. The 16S rRNA sequencing based metabarcoding approach revealed the enrichment of anammox bacteria (Candidatus Kuenenia), particularly in AS (0.016–0.021%) added CWs. The coexistence of nitrifiers (Nitrosospira, Nitrospira), heterotrophic and aerobic denitrifiers (Pseudomonas, Thauera, Bacillus), nitrate reducing bacteria (Clostridium, Enterobacter) and sulfur oxidizing bacteria (Halothiobacillus, Thiobacillus, Thiothrix) in treatment layer (sand + biochar) reflects the interplay of multiple N transformation processes in the VFCWs. Biochar facilitated microbial colonization and enhanced redox stratification, thereby supporting these processes. Overall, sludge augmentation in biochar integrated VFCWs significantly improved N removal and altered dominant N transformation pathways, offering valuable insights for optimizing CWs treating low C/N ratio wastewater.

从低碳氮比废水中高效去除氮(N)仍然是一个重大挑战。本研究评估了污泥改性[厌氧（AnS）或活性污泥（AS）]对生物炭整合垂直流人工湿地（VFCWs）处理模拟低COD/N比（3.6和2）废水的长期（227天）性能的影响。第一阶段的TN去除率为30.59 ~ 94.97%，第二阶段为50.62 ~ 90.97%，其中接种as的CWs去除率最高。污泥添加增强了N的去除，有26.77-50.96%的N未计算，可能反映了微生物转化、气体排放和其他N损失。基于16S rRNA测序的元条形码方法显示厌氧氨氧化菌（Candidatus Kuenenia）富集，特别是在添加CWs的AS（0.016-0.021%）中。处理层（砂+生物炭）中硝化菌（亚硝基螺旋体、硝化螺旋体）、异养好氧反硝化菌（假单胞菌、Thauera、芽孢杆菌）、硝酸盐还原菌（Clostridium、Enterobacter）和硫氧化菌（Halothiobacillus、Thiobacillus、thiiothrix）的共存反映了VFCWs中多个N转化过程的相互作用。生物炭促进微生物定植和增强氧化还原分层，从而支持这些过程。总体而言，生物炭集成VFCWs中污泥的增加显著提高了N的去除效果，并改变了主要的N转化途径，为优化处理低碳氮比废水的cfcws提供了有价值的见解。

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引用次数: 0

Synergistic ultrasound and zero-valent iron pretreatment for enhanced waste activated sludge anaerobic fermentation: Mechanism elucidation, process optimization, and benefit analysis 超声与零价铁协同预处理强化污泥厌氧发酵：机理阐述、工艺优化及效益分析

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-11 DOI: 10.1016/j.ibiod.2025.106242

Yingte Li , Dingxun Ma , Minggang Liu , Yizhou Tu , Hongbin Chen , Xianchun Tang

This study employed synergistic ultrasound and zero-valent iron (ZVI) pretreatment to enhance waste activated sludge (WAS) anaerobic fermentation. Using response surface methodology (RSM) to systematically optimize the ultrasonic power, ultrasound time, and ZVI dosage, aiming to determine the optimal synergistic pretreatment conditions for enhancing subsequent anaerobic fermentation. The results indicated that pretreatment reduced the particle size of WAS, increased fractal dimension and zeta potential absolute value, and resulted in a higher specific surface area, promoting electrostatic repulsion, system stability, and microbial growth. Alpha diversity increased (as reflected by the Chao index), and functional microbiota involved in hydrolysis and acidification, such as Actinomycetota and Bacteroidota, were further enriched. Further confirmation through three-dimensional excitation-emission matrix (3D-EEM) spectroscopy and molecular weight distribution analysis of dissolved organic matter revealed that the synergistic pretreatment significantly enhanced sludge hydrolysis efficiency and released substantial biodegradable components, with a higher biological index (BIX) and lower humification index (HIX). Specific analysis demonstrated that a maximum soluble chemical oxygen demand (SCOD) concentration of 1632.99 mg/L in the pretreated supernatant was achieved at an ultrasonic power of 1.86 W/mL, an ultrasound time of 14.10 min, and a ZVI dosage of 0.38 g/L. Furthermore, the maximum volatile fatty acids (VFAs) production (1722.65 mg COD/L) during the fermentation period was attained at an ultrasonic power of 1.78 W/mL, an ultrasound time of 14.48 min, and a ZVI dosage of 0.38 g/L. Benefit analysis suggests that this optimized process demonstrates promising application potential, offering an efficient and cost-effective strategy for sludge resource recovery.

本研究采用超声与零价铁（ZVI）协同预处理的方法，加强了废活性污泥（WAS）的厌氧发酵。采用响应面法（RSM）对超声功率、超声时间和ZVI投加量进行系统优化，旨在确定促进后续厌氧发酵的最佳协同预处理条件。结果表明，预处理使WAS的粒径减小，分形维数和zeta电位绝对值增大，比表面积增大，有利于静电斥力的提高，有利于系统稳定性和微生物生长。α多样性增加（由Chao指数反映），参与水解和酸化的功能微生物群，如放线菌群和拟杆菌群进一步丰富。通过三维激发-发射矩阵（3D-EEM）光谱和溶解有机物分子量分布分析进一步证实，协同预处理显著提高了污泥水解效率，释放了大量可生物降解组分，具有较高的生物指数（BIX）和较低的腐殖化指数（HIX）。具体分析表明，在超声功率为1.86 W/mL、超声时间为14.10 min、ZVI用量为0.38 g/L的条件下，预处理上清液中可溶化学需氧量（SCOD）的最大浓度为1632.99 mg/L。在超声功率为1.78 W/mL、超声时间为14.48 min、ZVI用量为0.38 g/L的条件下，发酵过程中挥发性脂肪酸（VFAs）的最大产量为1722.65 mg COD/L。效益分析表明，该优化工艺具有良好的应用潜力，为污泥资源回收提供了一种高效、经济的策略。

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引用次数: 0

Enhancement of microbial biofilms against chloramphenicol stress by AHL-driven quorum sensing ahl驱动的群体感应增强微生物生物膜对氯霉素胁迫的抵抗作用

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-11 DOI: 10.1016/j.ibiod.2025.106241

Jia Zhou , Weilong Xu , Zhanbo Xu , Sihan Liu , Yifan Liu , Leizhen Zhao , Shuai Zhao , Jianhang Qu , Bingjie Ni

Quorum sensing (QS) is a promising strategy to positively regulate the physiochemical properties of microbiota and consequently enhance the performance of biofilm reactor, however, its role in strengthening microbial biofilms against chloramphenicol (CAP) stress has not yet been fully elucidated. In this study, an N-acyl homoserine lactones (AHLs)-mediated QS signaling molecule, N-hexanoyl-L-homoserine lactone (C6-HSL), was supplemented into a biological contact oxidation reactor (BCOR) to evaluate its effect on reactor performance and microbial community under CAP shock conditions. When the influent CAP concentration increased from 20 to 50 mg/L, the addition of 0.1 μM C6-HSL enhanced the CAP removal efficiency from 84 % to 94 % in the BCOR. Meanwhile, the C6-HSL supplementation markedly stimulated the secretion of extracellular polymeric substances (EPS), particularly leading to a twofold increase in polysaccharide (PS) content, which played a vital role in maintaining biofilm stability and improving tolerance to CAP stress. Metagenomic analysis further revealed that C6-HSL supplementation enriched Sphingopyxis, Hydrogenophaga, Sphingobium, the major CAP-degrading genera harboring the capO gene edcoding CAP oxidoreductase, and upregulated QS-related functional genes involved in microbial communication. These findings demonstrate that QS regulation can effectively reinforce pollutant removal under antibiotic stress, offering a potential strategy for engineering robust microbial consortia in antibiotic-contaminated wastewater treatment systems.

群体感应（Quorum sensing， QS）是一种积极调节微生物群理化性质从而提高生物膜反应器性能的有前景的策略，但其在增强微生物生物膜抗氯霉素（chloramphenicol， CAP）胁迫中的作用尚未完全阐明。本研究将n -酰基高丝氨酸内酯（AHLs）介导的QS信号分子n -己醇- l-高丝氨酸内酯（C6-HSL）添加到生物接触氧化反应器（BCOR）中，评估其在CAP冲击条件下对反应器性能和微生物群落的影响。当进水CAP浓度从20 mg/L增加到50 mg/L时，添加0.1 μM C6-HSL可使BCOR中CAP的去除率从84%提高到94%。同时，添加C6-HSL显著刺激了胞外聚合物质（EPS）的分泌，特别是使多糖（PS）含量增加2倍，这对维持生物膜稳定性和提高对CAP胁迫的耐受性起着至关重要的作用。宏基因组分析进一步发现，添加C6-HSL可使Sphingopyxis、Hydrogenophaga、Sphingobium等主要的CAP降解属（含有编码CAP氧化还原酶的capO基因）富集，并上调参与微生物通讯的qs相关功能基因。这些发现表明，QS调控可以有效地加强抗生素胁迫下的污染物去除，为在抗生素污染的废水处理系统中设计强大的微生物群落提供了潜在的策略。

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引用次数: 0

Hydrodynamic modelling of biofilm detachment via the Immersed Boundary Method 基于浸入边界法的生物膜分离流体动力学建模

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-10 DOI: 10.1016/j.ibiod.2025.106245

Jiankun Wang , Yumeng Fu , Jin Wu , Jin Li , Xiaoling Wang

Biofilm detachment under fluid shear stress is a critical yet poorly understood phenomenon in biomedical and environmental systems. This study develops a 2D biofilm detachment model using IB2d within the Immersed Boundary Method (IBM) framework. By discretizing the biofilm into small units composed of elastic elements and connecting them through elastic/viscoelastic components in our 2D simulations, we dynamically model fluid-structure interactions. The strain threshold fracture criterion was experimentally calibrated and used to simulate biofilm detachment. Numerical simulations in straight microchannels reveal the existence of a critical detachment modulus in elastic biofilm systems within our model, which varies depending on the initial biofilm configuration. Specifically, the critical detachment modulus of semi-circular biofilms is lower than that of rectangular biofilms, while biofilms with parallel units exhibit a lower critical detachment modulus compared to those with randomly distributed units in our setup. Mechanical analysis further demonstrates that the parallel arrangement of units optimizes the stress transmission pathway, enhancing biofilm structural integrity in flow fields within our 2D simulations. Fluid dynamics studies indicate that fluid shear forces play a decisive role in biofilm structural disruption, with complete detachment occurring within seconds at high flow rates (e.g., 97 % detachment within 3 s at u_Max = 1 × 10⁻³ m/s). Viscoelastic analysis reveals that low-viscosity biofilms fail to effectively aggregate units, whereas high-viscosity biofilms behave similarly to those with elastic unit connections in our 2D model. This work bridges micro-mechanics and macro-detachment behavior in a two-dimensional context, offering a predictive tool for biofilm management strategies.

流体剪切应力作用下的生物膜剥离是生物医学和环境系统中一个重要但尚未被充分了解的现象。本研究在浸入边界法（浸入边界法）框架内使用IB2d开发了一个二维生物膜脱离模型。通过将生物膜离散成由弹性元件组成的小单元，并在二维模拟中通过弹性/粘弹性元件将它们连接起来，我们动态地模拟了流体-结构相互作用。实验校准了应变阈值断裂准则，并将其用于模拟生物膜脱离。在直微通道中的数值模拟表明，在我们的模型中，弹性生物膜系统中存在临界脱离模量，该模量取决于初始生物膜配置。具体来说，半圆形生物膜的临界脱离模量低于矩形生物膜，而平行单元的生物膜的临界脱离模量低于随机分布单元的生物膜。力学分析进一步表明，单元的平行排列优化了应力传递途径，增强了二维模拟流场中生物膜结构的完整性。流体动力学研究表明，流体剪切力在生物膜结构破坏中起着决定性作用，在高流速下，几秒钟内就会发生完全分离（例如，在uMax = 1 × 10−3 m/s时，3秒内97%的分离）。粘弹性分析表明，低粘度生物膜不能有效地聚集单元，而高粘度生物膜的行为与我们二维模型中具有弹性单元连接的生物膜相似。这项工作在二维背景下将微观力学和宏观脱离行为联系起来，为生物膜管理策略提供了预测工具。

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引用次数: 0

Rhizosphere phenolics by urban plants drive laccase-like oxidative conditions and selective degradation of phenolic/anilinic antibiotics 城市植物的根际酚类物质驱动漆酶样氧化条件和酚类/苯胺类抗生素的选择性降解

IF 4.1 2区环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Biodeterioration & Biodegradation

Pub Date : 2025-11-09 DOI: 10.1016/j.ibiod.2025.106243

Zhimin Zhou , Shuzhuang Wu , Junchao Huang , Yuxin Chen , Yongpeng Li , Baobao Cai , Pengkang Yan , Dionisio Zaldivar-Silva , Shaojie Liu , Weiyi Lv , Bangxiao Zheng

Antibiotics increasingly occur at urban root–soil–water interfaces, yet the proximate chemical driver of any in-situ dissipation remains unclear. We tested the hypothesis that phenolic root exudates establish laccase-like oxidative conditions that selectively shorten the persistence of phenolic or anilinic antibiotics. Using planted microcosms spanning a gradient in exudate phenolics, paired with complementary exudate assays with copper chelation and dephenolization, we quantified H₂O₂-free oxidative activity and first-order decay of tetracycline, sulfamethoxazole, and a recalcitrant comparator, carbamazepine. High-phenolic rhizospheres produced the strongest extracellular oxidation and yielded two-to fourfold shorter half-lives for tetracycline and sulfamethoxazole, whereas carbamazepine was unchanged. Across treatments, oxidative activity was strongly and inversely associated with half-life for the phenolic or anilinic antibiotics and a structural model linked phenolic exudation to oxidation and then to antibiotic persistence, explaining 76 % of the variance, while the same conditions coincided with lower abundances of resistance markers and higher bacterial evenness after two weeks. These findings verify the laccase-like rhizosphere mechanism and identify exudate chemistry as a practical lever for selective antibiotic dissipation and risk reduction in urban green infrastructures.

抗生素越来越多地出现在城市根-土壤-水界面，但任何原位耗散的近似化学驱动因素尚不清楚。我们检验了酚根渗出物建立漆酶样氧化条件的假设，选择性地缩短酚类或苯胺类抗生素的持续时间。通过在渗出物酚类物质中设置梯度的种植显微镜，结合铜螯合和去酚化的互补渗出物测定，我们定量了四环素、磺胺甲恶唑和顽固性比较物卡马西平的无h2o2氧化活性和一级衰变。高酚根际产生最强的细胞外氧化作用，使四环素和磺胺甲恶唑的半衰期缩短2 - 4倍，而卡马西平没有变化。在不同的处理中，氧化活性与酚类或苯胺类抗生素的半衰期呈强烈的负相关，并且结构模型将酚类分泌物与氧化联系起来，然后与抗生素持久性联系起来，解释了76%的差异，而相同的条件下，两周后耐药标记物的丰度较低，细菌均匀性较高。这些发现证实了漆酶样根际机制，并确定了渗出化学是城市绿色基础设施中选择性抗生素消散和降低风险的实用杠杆。

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引用次数: 0