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A Novel Prehydrated Porcine-Derived Acellular Dermal Matrix: A Histological and Clinical Evaluation. 一种新颖的预脱水猪肝细胞真皮基质:组织学和临床评估
IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-06-27 eCollection Date: 2024-01-01 DOI: 10.1155/2024/7322223
Andreas van Orten, Werner Goetz, Hakan Bilhan

It is well known that soft tissue quality and quantity around dental implants is of paramount importance for later peri-implant health. For this purpose, the clinical and histological outcomes of the peri-implant mucosa, following soft tissue augmentation for soft tissue improvement with a novel prehydrated porcine acellular dermal matrix graft (PPADMG) in conjunction with simultaneous implant placement, were evaluated in this case series. Twenty-two patients were included in the study. They underwent a late implant placement protocol combined with PPADMG for soft tissue augmentation. A punch biopsy was taken at the time of uncovery of the submerged healed implant after a mean of 157 days healing time. Supracrestal soft tissue height (STH) was measured at the time of implant placement and uncovery. All sites showed a clinical increase in STH. The histological structure of the biopsies resembled a similar structure as found in the healthy oral mucosa. No unexpected tissue reactions could be found. Within the limits of this clinical and histological study, it may be concluded that STH improvement with this novel porcine-derived acellular dermal matrix, in combination with simultaneous implant placement, is a viable option to create a peri-implant tissue thickness and stability.

众所周知,牙科种植体周围软组织的质量和数量对日后种植体周围的健康至关重要。为此,本病例系列评估了使用新型预水化猪无细胞真皮基质移植体(PPADMG)进行软组织增量以改善软组织并同时植入种植体后,种植体周围粘膜的临床和组织学效果。研究共纳入了 22 名患者。他们接受了晚期种植体植入方案,并结合 PPADMG 进行了软组织增量。在平均 157 天的愈合时间后,在水下愈合的种植体未恢复时进行了打孔活检。在植入种植体和拔出种植体时测量了胸骨上软组织高度(STH)。所有部位的 STH 都出现了临床增长。活组织切片的组织学结构与健康口腔粘膜的结构相似。没有发现意外的组织反应。在这项临床和组织学研究的范围内,可以得出这样的结论:使用这种新型的孔源性非细胞真皮基质,结合同时植入的种植体,可以改善 STH,从而增加种植体周围组织的厚度和稳定性。
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引用次数: 0
The Influence of Thickness on the Mechanical Behaviors of 3D Printing Resins for Orthodontic Retainers. 厚度对用于正畸的 3D 打印树脂机械行为的影响
IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS Pub Date : 2024-06-25 eCollection Date: 2024-01-01 DOI: 10.1155/2024/7398478
Theerasak Nakornnoi, Patamaporn Bunjerdjin, Peerapong Santiwong, Kawin Sipiyaruk, Siew Peng Neoh, Rochaya Chintavalakorn

This study aimed to evaluate the mechanical behaviors of thermoformed and 3D-printed retainers with different thicknesses. Thermoformed retainers (Duran) and 3D-printed retainers (Dental LT Clear V2 and NextDent Ortho Flex) were fabricated at thicknesses of 0.5, 0.75, and 1 mm. Five samples of each material were subjected to compression, tensile, and flexural testing with the universal testing machine (Instron Ltd., Buckinghamshire, England). The results revealed that the mechanical behaviors were significantly influenced by thickness in each type of material. The increased thickness tended to increase strength and modulus in all three tests. However, Dental LT Clear V2 and Duran showed that flexural strength and modulus were inversely related to thickness. The compressive test revealed significantly greater compressive resistance in 3D-printed groups, except for the NextDent Ortho Flex at 0.5 mm. The tensile test showed that Dental LT Clear V2 at all thicknesses demonstrated significantly higher tensile strength and modulus, while NextDent Ortho Flex was significantly lowest at any thickness in tensile and flexural properties. In conclusion, the thickness significantly influenced the mechanical behaviors of the 3D-printed retainers. The 0.75 mm thickness of Dental LT Clear V2 could be considered as an alternative to fabricated retainers due to its similar mechanical properties compared with the thermoformed material.

本研究旨在评估不同厚度的热成型和三维打印固位体的机械性能。热成型固位体(Duran)和三维打印固位体(Dental LT Clear V2 和 NextDent Ortho Flex)的厚度分别为 0.5、0.75 和 1 毫米。使用万能试验机(Instron Ltd., Buckinghamshire, England)对每种材料的五个样品进行了压缩、拉伸和弯曲测试。结果表明,每种材料的机械性能都受到厚度的显著影响。在所有三项测试中,厚度增加往往会提高强度和模量。然而,牙科 LT Clear V2 和 Duran 显示,抗弯强度和模量与厚度成反比。抗压测试表明,除 0.5 毫米的 NextDent Ortho Flex 外,3D 打印组的抗压性能明显更强。拉伸测试表明,所有厚度的 Dental LT Clear V2 拉伸强度和模量都明显更高,而任何厚度的 NextDent Ortho Flex 拉伸和弯曲性能都明显最低。总之,厚度对 3D 打印固位体的机械性能有很大影响。与热成型材料相比,厚度为 0.75 毫米的 Dental LT Clear V2 具有相似的机械性能,因此可作为制造固位体的替代材料。
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引用次数: 0
Impact of Artificial Aging on the Physical and Mechanical Characteristics of Denture Base Materials Fabricated via 3D Printing. 人工老化对通过 3D 打印制作的义齿基底材料的物理和机械特性的影响。
IF 3 Q2 Engineering Pub Date : 2024-06-18 eCollection Date: 2024-01-01 DOI: 10.1155/2024/8060363
Ahmed Altarazi, Julfikar Haider, Abdulaziz Alhotan, Nick Silikas, Hugh Devlin

Three-dimensional (3D) printing is becoming more prevalent in the dental sector due to its potential to save time for dental practitioners, streamline fabrication processes, enhance precision and consistency in fabricating prosthetic models, and offer cost-effective solutions. However, the effect of aging in artificial saliva of this type of material has not been explored. To assess the physical and mechanical properties of the two types of 3D-printed materials before and after being subjected to artificial saliva, a total of 219 acrylic resin specimens were produced. These specimens were made with two types of 3D-printed materials, namely, NextDent (ND) and Formlabs (FLs), and a Schottlander heat-cured (HC) resin material that was used as a control. Water sorption and solubility specimens (n = 5) were tested after three months of storage in artificial saliva. Moreover, the Vickers hardness, Martens hardness, flexural strength/modulus, and impact strength were evaluated both under dry conditions and after three months of storage in artificial saliva. The degree of conversion (DC), elemental analysis, and filler content were also investigated. The ANOVA showed that 3D-printed resins had significantly greater sorption than the control group (p < 0.05). However, the flexural strength values of the 3D-printed materials were significantly greater (p < 0.05) than those of the heat-cured material. The DC of the 3D-printed resins was lower than that of the control group, but the difference was not significant (p > 0.05). The 3D-printed materials contained significantly more filler than the control (p < 0.05). Moreover, the artificial saliva had a significant effect on the Vickers hardness for all tested groups and on the Martens hardness for the control group only (p < 0.05). Compared with conventional heat-cured materials, 3D-printed denture base materials demonstrated relatively poorer performance in terms of sorption, solubility, and DC but exhibited either comparable or superior mechanical properties. The aging process also influenced the Vickers and Martens' hardness. The strength of the 3D-printed materials was in compliance with ISO recommendations, and the materials could be used alongside conventional heat-cured materials.

三维(3D)打印技术可以为牙科医生节省时间、简化制作流程、提高制作修复模型的精度和一致性,并提供具有成本效益的解决方案,因此在牙科领域正变得越来越普遍。然而,这类材料在人工唾液中的老化效应尚未得到研究。为了评估两种 3D 打印材料在人工唾液中老化前后的物理和机械性能,共制作了 219 个丙烯酸树脂试样。这些试样由两种 3D 打印材料制成,即 NextDent(ND)和 Formlabs(FLs),以及作为对照的 Schottlander 热固化(HC)树脂材料。在人工唾液中存放三个月后,对吸水性和溶解性试样(n = 5)进行了测试。此外,还对干燥条件下和在人工唾液中存放三个月后的维氏硬度、马氏硬度、抗弯强度/模量和冲击强度进行了评估。此外,还对转化率(DC)、元素分析和填料含量进行了研究。方差分析结果表明,三维打印树脂的吸附力明显高于对照组(p < 0.05)。不过,三维打印材料的抗弯强度值明显高于热固化材料(p < 0.05)。三维打印树脂的直流电低于对照组,但差异不显著(p > 0.05)。三维打印材料所含的填料明显多于对照组(p < 0.05)。此外,人工唾液对所有测试组的维氏硬度都有显著影响,仅对对照组的马氏硬度有显著影响(p < 0.05)。与传统的热固化材料相比,三维打印义齿基底材料在吸附性、溶解性和直流电方面的性能相对较差,但在机械性能方面却具有可比性或优越性。老化过程也会影响维氏硬度和马氏硬度。三维打印材料的强度符合 ISO 建议,可与传统热固化材料一起使用。
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引用次数: 0
Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression. 通过表达 OPG、RANK 和 RANKL,用纳米颗粒稻壳液体烟雾治疗牙周炎。
IF 3 Q2 Engineering Pub Date : 2024-06-14 eCollection Date: 2024-01-01 DOI: 10.1155/2024/5015893
Ira Arundina, Theresia Indah Budhy, Aqsa Sjuhada Oki, Meircurius Dwi Condro Surboyo, Arvind Babu Rajendra Santosh, Sidarningsih, Indeswati Diyatri, Tytania Rahmaputry, Arya Pradana, Mohammad Iqbal, Azzahra Salsabila Adira Moelyanto

Introduction: Periodontitis therapy employing nanomaterials with submicron sizes holds promise for enhancing osteogenesis and facilitating periodontal cell proliferation. This study aims to assess the potential of nanoparticle-based rice husk liquid smoke (n-RHLS) in an animal model of periodontitis by evaluating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kβ (RANK), and receptor activator of nuclear factor-kβ ligand (RANKL).

Methods: Twenty-eight male Wistar rats were inoculated with 109 CFU/ml of Porphyromonas gingivalis in the sulcus mandibular incisor region to create periodontitis and subsequently treated with n-RHLS while the control with saline. Immunohistochemical staining was performed on the mandibular incisor to assess OPG, RANK, and RANKL expression 2 and 7 days after treatment.

Results: OPG expression exhibited a significant increase at both 2 and 7 days, while RANKL expression decreased notably after 7 days of treatment using n-RHLS (p < 0.05). In contrast, RANK expression did not show significant differences compared to the control groups (p > 0.05).

Conclusion: Nanostructured liquid smoke derived from rice husk nanoparticles (n-RHLS) demonstrates potential as a therapeutic agent for periodontitis, especially on OPG/RANK/RANKL expression, by modulating OPG and RANKL expression to support periodontal tissue health.

导言:采用亚微米级纳米材料治疗牙周炎有望增强骨生成并促进牙周细胞增殖。本研究旨在通过评估骨保护素(OPG)、核因子-kβ受体激活剂(RANK)和核因子-kβ受体激活剂配体(RANKL)的表达,评估基于纳米颗粒的稻壳液体烟雾(n-RHLS)在牙周炎动物模型中的潜力:将 109 CFU/ml 的牙龈卟啉单胞菌接种到 28 只雄性 Wistar 大鼠的下颌切牙沟区域,造成牙周炎,然后用 n-RHLS 治疗,用生理盐水对照。治疗后 2 天和 7 天,对下颌切牙进行免疫组化染色,以评估 OPG、RANK 和 RANKL 的表达:结果:OPG 的表达在治疗 2 天和 7 天后都有明显增加,而 RANKL 的表达在使用 n-RHLS 治疗 7 天后明显下降(p < 0.05)。相比之下,RANK 的表达与对照组相比没有明显差异(p > 0.05):结论:稻壳纳米颗粒衍生的纳米结构液态烟雾(n-RHLS)具有治疗牙周炎的潜力,尤其是通过调节OPG/RANK/RANKL的表达来支持牙周组织的健康。
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引用次数: 0
New Nanosized V(III), Fe(III), and Ni(II) Complexes Comprising Schiff Base and 2-Amino-4-Methyl Pyrimidine: Synthesis, Properties, and Biological Activity 包含希夫碱和 2-氨基-4-甲基嘧啶的新型纳米级 V(III)、Fe(III) 和 Ni(II) 配合物:合成、性质和生物活性
IF 3.1 Q2 Engineering Pub Date : 2024-05-14 DOI: 10.1155/2024/9198129
Maged S. Al-Fakeh, Maha A. Alsikhan, J. Alnawmasi, Abdullah H. Alluhayb, Mona S. Al-Wahibi
A new synthesis of mixed ligand complexes vanadium(III), iron(III), and nickel(II), [M : L1 : L2], where L1 = Schiff base 2-((E)-((4-(((E)-benzylidene)amino)phenyl)imino)methyl)-naphthalene-1-ol (C24H18N2O) as for L2 = AMPY 2-amino-4-methyl pyrimidine (C5H7N3) were prepared in powder and investigated. Element analysis, molar conductivity, FT-IR, UV-vis, and magnetic susceptibility values have been acquired to describe the generated complexes. The values of vanadium(III), iron(III), and nickel(II) compounds are, respectively, 2.88 BM, 5.96 BM, and 2.92 BM, demonstrating that all compounds conform to the recommended octahedral geometry. Thermal gravimetric analysis (TGA) is used to further assess the complexes and establish the temperature stability and degradation of the metal complexes. The calculations abstracted from XRD patterns propose nanosized complexes (average size 29–50 nm). The microstructures of the samples have also been investigated by scanning electron microscopy (SEM). The disc diffusion method was used to assess and analyze the inhibition of the growth of compounds against harmful bacterial and fungal strains. The prepared complexes were tested against three strains of bacteria, one gram-positive strain (Bacillus subtilis), two gram-negative strains (Escherichia coli and Pseudomonas aeruginosa), and one fungus (Aspergillus fumigatus). The complexes inferred antimicrobial activity against the studied organisms. Specifically, vanadium(III) and nickel(II) are more effective than iron(III), making them promising drugs.
一种新的混合配体钒(III)、铁(III)和镍(II)络合物[M : L1 : L2]的合成方法以粉末状制备并研究了 L1 = 席夫碱 2-((E)-((4-(((E)-亚苄基)氨基)苯基)亚氨基)甲基)-1-萘酚(C24H18N2O)和 L2 = AMPY 2-氨基-4-甲基嘧啶(C5H7N3)。对生成的配合物进行了元素分析、摩尔电导率、傅立叶变换红外光谱、紫外-可见光谱和磁感应强度测定。钒(III)、铁(III)和镍(II)化合物的数值分别为 2.88 BM、5.96 BM 和 2.92 BM,表明所有化合物都符合推荐的八面体几何形状。热重分析(TGA)用于进一步评估复合物,并确定金属复合物的温度稳定性和降解情况。根据 X 射线衍射图计算得出的结果表明,络合物的尺寸为纳米级(平均尺寸为 29-50 纳米)。样品的微观结构也通过扫描电子显微镜(SEM)进行了研究。盘扩散法用于评估和分析化合物对有害细菌和真菌菌株生长的抑制作用。制备的复合物针对三株细菌进行了测试,其中一株为革兰氏阳性菌(枯草芽孢杆菌),两株为革兰氏阴性菌(大肠杆菌和绿脓杆菌),一株为真菌(曲霉菌)。这些配合物对所研究的生物具有抗菌活性。具体来说,钒(III)和镍(II)比铁(III)更有效,因此是很有前途的药物。
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引用次数: 0
Enhancing Presurgical Infant Orthopedic Appliances: Characterization, Mechanics, and Biofilm Inhibition of a Novel Chlorhexidine-Halloysite Nanotube-Modified PMMA 增强手术前婴儿矫形器:新型氯己定合金纳米管改性聚甲基丙烯酸甲酯的特性、力学和生物膜抑制作用
IF 3.1 Q2 Engineering Pub Date : 2024-05-10 DOI: 10.1155/2024/6281972
Nadia Al Ansari, Mushriq F Abid
Objectives. This in vitro study aimed to develop a novel nanocomposite acrylic resin with inherent antimicrobial properties. This study evaluated its effectiveness against microbial biofilm formation, while also assessing its physical and mechanical properties. Methods. Polymethylmethacrylate (PMMA) was modified with four different concentrations of chlorhexidine halloysite nanotubes (CHX-HNTs): 1%, 1.5%, 3%, and 4.5 wt.% by weight, along with a control group (0 wt.% CHX-HNTs). The biofilm inhibition ability of the modified CHX-HNTs acrylic against Candida albicans, Staphylococcus aureus, Streptococcus pneumoniae, and Streptococcus agalactiae was assessed using microtiter biofilm test. In addition, ten samples from each group were then tested for flexural strength, surface roughness, and hardness. Statistical analysis was performed using one-way ANOVA and Tukey’s test for comparison (P<0.05). Results. CHX-HNTs effectively reduced the adhesion of Candida albicans and bacteria to the PMMA in a dose-dependent manner. The higher the concentration of CHX-HNTs, the greater the reduction in microbial adhesion, with the highest concentration (4.5 wt.%) showing the most significant effect with inhibition rates ≥98%. The addition of CHX-HNTs at any tested concentration (1%, 1.5%, 3%, and 4.5 wt.%) did not cause any statistically significant difference in the flexural strength, surface roughness, or hardness of the PMMA compared to the control group. Conclusions. The novel integration of CHX-HNT fillers shows promising results as an effective biofilm inhibitor on acrylic appliances. This new approach has the potential to successfully control infectious diseases without negatively affecting the mechanical properties of the acrylic resin. Clinical Relevance. The integration of CHX-HNTs into presurgical infant orthopedic appliances should be thoroughly assessed as a promising preventive measure to mitigate microbial infections. This evaluation holds significant potential for controlling infectious diseases among infants with cleft lip and palate, thereby offering a valuable contribution to their overall well-being.
研究目的这项体外研究旨在开发一种具有固有抗菌特性的新型纳米复合丙烯酸树脂。本研究评估了它对微生物生物膜形成的有效性,同时还评估了它的物理和机械性能。方法:聚甲基丙烯酸甲酯在聚甲基丙烯酸甲酯(PMMA)中加入四种不同浓度的洗必泰哈洛石纳米管(CHX-HNTs):按重量计分别为 1%、1.5%、3% 和 4.5%,同时加入对照组(CHX-HNTs 含量为 0)。利用微滴定生物膜试验评估了改性 CHX-HNTs 丙烯酸盐对白色念珠菌、金黄色葡萄球菌、肺炎链球菌和无乳链球菌的生物膜抑制能力。此外,还对每组的十个样品进行了抗折强度、表面粗糙度和硬度测试。统计分析采用单因素方差分析和 Tukey 检验进行比较(P<0.05)。结果CHX-HNTs 能有效减少白色念珠菌和细菌在 PMMA 上的粘附,其效果与剂量有关。CHX-HNTs 的浓度越高,微生物附着力的降低幅度越大,其中最高浓度(4.5 wt.%)的效果最显著,抑制率≥98%。与对照组相比,以任何测试浓度(1%、1.5%、3% 和 4.5 wt.%)添加 CHX-HNTs 都不会导致 PMMA 的抗弯强度、表面粗糙度或硬度出现任何统计学上的显著差异。结论CHX-HNT填料的新型整合作为一种有效的生物膜抑制剂,在丙烯酸器械上显示出良好的效果。这种新方法有可能在不对丙烯酸树脂的机械性能产生负面影响的情况下成功控制感染性疾病。临床意义。应全面评估将 CHX-HNTs 整合到手术前婴儿矫形器具中的效果,将其作为一种有希望减轻微生物感染的预防措施。这项评估在控制唇腭裂婴儿感染性疾病方面具有巨大潜力,从而为他们的整体健康做出宝贵贡献。
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引用次数: 0
Alginate Nanoparticles Containing Cuminum cyminum and Zataria multiflora Essential Oils with Promising Anticancer and Antibacterial Effects. 含有孜然和多花蝙蝠葛精油的藻酸盐纳米粒子具有良好的抗癌和抗菌效果
IF 3.1 Q2 Engineering Pub Date : 2024-05-02 eCollection Date: 2024-01-01 DOI: 10.1155/2024/5556838
Mahmoud Osanloo, Razieh Ranjbar, Elham Zarenezhad

Cancer and bacterial infections are major global health concerns driving the need for innovative medicines. This study investigated alginate nanoparticles loaded with essential oils (EOs) from Cuminum cyminum and Zataria multiflora as potential drug delivery systems. The nanoparticles were comprehensively characterized using techniques such as gas chromatography-mass spectrometry (GC-MS), dynamic light scattering (DLS), zetasizer, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), and ultraviolet-visible spectroscopy (UV-Vis). Their biological properties against two human skin cancer cell lines (A-375 and A-431) and three bacteria (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) were also evaluated. Alginate nanoparticles containing C. cyminum and Z. multiflora EOs exhibited sizes of 160 ± 8 nm and 151 ± 10 nm, respectively. Their zeta potentials and encapsulation efficiencies were -18 ± 1 mV and 79 ± 4%, as well as -27 ± 2 mV and 86 ± 5%, respectively. The IC50 values against the tested cell lines and bacteria revealed superior efficacy for nanoparticles containing Z. multiflora EO. Considering the proper efficacy of the proposed nanoparticles, the straightforward preparation method and low cost suggest their potential for further in vivo studies.

癌症和细菌感染是全球关注的主要健康问题,推动了对创新药物的需求。本研究探讨了藻酸盐纳米颗粒作为潜在的药物输送系统,其中装载了孜然和多花蓼的精油(EO)。研究采用气相色谱-质谱(GC-MS)、动态光散射(DLS)、zetasizer、衰减全反射-傅立叶变换红外光谱(ATR-FTIR)和紫外-可见光谱(UV-Vis)等技术对纳米颗粒进行了全面表征。此外,还评估了它们对两种人类皮肤癌细胞系(A-375 和 A-431)和三种细菌(大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌)的生物特性。藻酸盐纳米颗粒含有西米露和多花植物萃取精华,尺寸分别为 160 ± 8 nm 和 151 ± 10 nm。它们的 zeta 电位和封装效率分别为 -18 ± 1 mV 和 79 ± 4%,以及 -27 ± 2 mV 和 86 ± 5%。对测试细胞系和细菌的 IC50 值显示,含有多花梓树环氧乙烷的纳米粒子具有卓越的功效。考虑到所提出的纳米颗粒具有适当的功效,其简单的制备方法和低廉的成本表明其具有进一步进行体内研究的潜力。
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引用次数: 0
Development of Intelligent Indicators Based on Cellulose and Prunus domestica Extracted Anthocyanins for Monitoring the Freshness of Packaged Chicken 开发基于纤维素和梅花提取花青素的智能指标,用于监测包装鸡肉的新鲜度
IF 3.1 Q2 Engineering Pub Date : 2024-03-28 DOI: 10.1155/2024/7949258
Mustafa Ahmed, Ipsheta Bose, Nousheen, Swarup Roy
Meat is a widely consumed food globally; however, variations in storage conditions along its supply chain can pose a potential food safety risk for consumers. Addressing this concern, we have developed freshness indicators designed to monitor the condition of packaged chicken. In this study, anthocyanins were infused with cellulose paper measuring 2 × 2 cm, and subsequent analysis focused on examining color changes concerning deteriorating chicken stored at 30°C for 48 h, with varying sample sizes being considered. The rise in total volatile nitrogen (TVB-N) compounds from an initial value of 3.64 ± 0.39 mg/100 g to 28.17 ± 1.46 mg/100 g acted as the stimulus for the color change in the indicator, simultaneously influencing the pH from the initial 7.03 ± 0.16 to 8.12 ± 0.39. The microbial load (aerobic plate count) of the chicken samples was also significantly increased. This collective shift in various parameters strongly suggests the occurrence of spoilage in chicken meat. The pH indicators exhibited a dark pink to red color for fresh chicken. As the chicken meat turned towards spoilage, the indicators changed to a dark blue and then a pale green color. FTIR spectroscopy results confirmed the presence of cellulose and anthocyanins. The FTIR analysis also validated the immobilization of plum anthocyanins within the cellulose paper and assessed their stability after 8 months of storage. Notably, the indicators demonstrated rapid sensitivity, showing a 20.5% response within one minute of ammonia exposure, which further increased to 29.5% after 3 min of exposure. The total color difference (ΔE) steadily rose in all the examined samples and also under various storage conditions. Overall, the indicators developed in this study exhibited a highly pronounced color transition, capable of distinguishing between fresh and spoiled chicken samples depending on the extent of spoilage and the specific day of observation.
肉类是全球广泛消费的食品;然而,其供应链上储存条件的变化会给消费者带来潜在的食品安全风险。针对这一问题,我们开发了新鲜度指标,用于监测包装鸡肉的状况。在这项研究中,花青素被注入 2 × 2 厘米的纤维素纸中,随后的分析重点是检测在 30°C 下存放 48 小时的变质鸡肉的颜色变化,并考虑不同的样本量。总挥发性氮化合物(TVB-N)从最初的 3.64 ± 0.39 mg/100 g 增加到 28.17 ± 1.46 mg/100 g,刺激了指示剂颜色的变化,同时影响了 pH 值,从最初的 7.03 ± 0.16 增加到 8.12 ± 0.39。鸡肉样品中的微生物量(需氧菌落总数)也显著增加。各种参数的集体变化强烈表明鸡肉发生了腐败。新鲜鸡肉的 pH 指标呈深粉红色至红色。随着鸡肉变质,指示剂变为深蓝色,然后是淡绿色。傅立叶变换红外光谱分析结果证实了纤维素和花青素的存在。傅立叶变换红外光谱分析还验证了李花青素在纤维素纸中的固定性,并评估了其在储存 8 个月后的稳定性。值得注意的是,指示剂显示出快速的灵敏度,在接触氨气一分钟内显示出 20.5% 的反应,接触 3 分钟后进一步增加到 29.5%。总色差(ΔE)在所有检测样品和各种储存条件下都稳步上升。总之,本研究开发的指标表现出非常明显的颜色变化,能够根据腐败程度和特定的观察日期区分新鲜和腐败鸡肉样品。
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引用次数: 0
Improving Biocompatibility of Polyurethanes Apply in Medicine Using Oxygen Plasma and Its Negative Effect on Increased Bacterial Adhesion. 利用氧等离子体改善医用聚氨酯的生物相容性及其对增加细菌粘附性的负面影响
IF 3.1 Q2 Engineering Pub Date : 2024-02-23 eCollection Date: 2024-01-01 DOI: 10.1155/2024/5102603
Kamil Drożdż, Monika Gołda-Cępa, Paulina Chytrosz-Wróbel, Andrzej Kotarba, Monika Brzychczy-Włoch

Polyurethanes (PUs) are versatile polymers used in medical applications due to their high flexibility and fatigue resistance. PUs are widely used for synthetic blood vessels, wound dressings, cannulas, and urinary and cardiovascular catheters. Many scientific reports indicate that surface wettability is crucial for biocompatibility and bacterial adhesion. The use of oxygen plasma to modify PUs is advantageous because of its effectiveness in introducing oxygen-containing functional groups, thereby altering surface wettability. The purpose of this study was to investigate the effect of the modification of the oxygen plasma of polyurethane on its biocompatibility with lung tissue (A549 cell line) and the adhesion of Gram-positive bacteria (S. aureus and S. epidermidis). The results showed that the modification of polyurethane by oxygen plasma allowed the introduction of functional groups containing oxygen (-OH and -COOH), which significantly increased its hydrophilicity (change from 105° ± 2° to 9° ± 2°) of PUs. Surface analysis by atomic force microscopy (AFM) showed changes in PU topography (change in maximum height from ∼110.3 nm to ∼32.1 nm). Moreover, biocompatibility studies on A549 cells showed that on the PU-modified surface, the cells exhibited altered morphology (increases in cell surface area and length, and thus reduced circularity) without concomitant effects on cell viability. However, serial dilution and plate count and microscopic methods confirmed that plasma modification significantly increased the adhesion of S. aureus and S. epidermidis bacteria. This study indicate the important role of surface hydrophilicity in biocompatibility and bacterial adhesion, which is important in the design of new medical biomaterials.

聚氨酯(PU)具有高柔韧性和抗疲劳性,是一种用于医疗领域的多功能聚合物。聚氨酯被广泛用于合成血管、伤口敷料、插管、导尿管和心血管导管。许多科学报告表明,表面润湿性对生物相容性和细菌粘附性至关重要。使用氧等离子体改性聚氨酯的优势在于它能有效引入含氧官能团,从而改变表面润湿性。本研究的目的是研究氧等离子体对聚氨酯的改性对其与肺组织(A549 细胞系)的生物相容性和革兰氏阳性细菌(金黄色葡萄球菌和表皮葡萄球菌)的粘附性的影响。结果表明,通过氧等离子体对聚氨酯进行改性,可引入含氧(-OH 和 -COOH)的官能团,从而显著提高聚氨酯的亲水性(从 105° ± 2° 变为 9° ± 2°)。原子力显微镜(AFM)的表面分析表明,聚氨酯的形貌发生了变化(最大高度从 110.3 纳米变为 32.1 纳米)。此外,对 A549 细胞进行的生物相容性研究表明,在聚氨酯改性表面上,细胞的形态发生了改变(细胞表面积和长度增加,因而圆形度降低),但细胞活力没有受到影响。然而,连续稀释、平板计数和显微镜方法证实,等离子体改性显著增加了金黄色葡萄球菌和表皮葡萄球菌的粘附力。这项研究表明了表面亲水性在生物相容性和细菌粘附性中的重要作用,这对新型医用生物材料的设计非常重要。
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引用次数: 0
Current Strategies for Tracheal Decellularization: A Systematic Review. 气管脱细胞的当前策略:系统回顾
IF 3.1 Q2 Engineering Pub Date : 2024-02-06 eCollection Date: 2024-01-01 DOI: 10.1155/2024/3355239
Dhihintia Jiwangga, Ferdiansyah Mahyudin, Gondo Mastutik, Juliana, Estya Nadya Meitavany

The process of decellularization is crucial for producing a substitute for the absent tracheal segment, and the choice of agents and methods significantly influences the outcomes. This paper aims to systematically review the efficacy of diverse tracheal decellularization agents and methods using the PRISMA flowchart. Inclusion criteria encompassed experimental studies published between 2018 and 2023, written in English, and detailing outcomes related to histopathological anatomy, DNA quantification, ECM evaluation, and biomechanical characteristics. Exclusion criteria involved studies related to 3D printing, biomaterials, and partial decellularization. A comprehensive search on PubMed, NCBI, and ScienceDirect yielded 17 relevant literatures. The integration of various agents and methods has proven effective in the process of tracheal decellularization, highlighting the distinct advantages and drawbacks associated with each agent and method.

脱细胞过程对于制作缺失气管段的替代物至关重要,而药剂和方法的选择对结果有很大影响。本文旨在利用PRISMA流程图系统回顾各种气管脱细胞制剂和方法的疗效。纳入标准包括 2018 年至 2023 年间发表的实验研究,以英文撰写,详细介绍了与组织病理学解剖、DNA 定量、ECM 评估和生物力学特征相关的结果。排除标准包括与 3D 打印、生物材料和部分脱细胞相关的研究。通过在 PubMed、NCBI 和 ScienceDirect 上进行全面搜索,共获得 17 篇相关文献。在气管脱细胞的过程中,各种药剂和方法的整合被证明是有效的,同时也突出了每种药剂和方法的明显优势和缺点。
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引用次数: 0
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International Journal of Biomaterials
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