Pub Date : 2020-06-14DOI: 10.14419/ijac.v8i1.30723
M. Mohammed El Amine, A. E. Ghanjaoui, A. Mandil, A. Ait Sidi Mou, R. Slimani
This review gives a general overview of High Performance Liquid Chromatography (HPLC) Method for the detection, the separation and the quantification of the active compounds from the organics matrices. A brief description of the instrumentation and the method devel-opment is provided. The principles of HPLC including different separation modes and detection methods for the quantitative analysis are summarized. Finely, the validation procedures in real samples are also described.
{"title":"High performance liquid chromatography quality control","authors":"M. Mohammed El Amine, A. E. Ghanjaoui, A. Mandil, A. Ait Sidi Mou, R. Slimani","doi":"10.14419/ijac.v8i1.30723","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30723","url":null,"abstract":"This review gives a general overview of High Performance Liquid Chromatography (HPLC) Method for the detection, the separation and the quantification of the active compounds from the organics matrices. A brief description of the instrumentation and the method devel-opment is provided. The principles of HPLC including different separation modes and detection methods for the quantitative analysis are summarized. Finely, the validation procedures in real samples are also described. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82800486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-04DOI: 10.14419/ijac.v8i1.30526
Alhassan S.I, Sule S.Y, M. R., R. H.I, Baso A.A, Sheshe F.A, Jafar S.C
Acetylation of gum Arabic was achieved using acetic anhydride as solvent. The ester group formed was confirmed by FTIR spectra having absorption band of 750 cm-1 – 700 cm-1. Viscometric study of the pure and acetylated samples was carried out. Relative viscosity of acetylated gum was found to be higher than that of the pure gum. Intrinsic viscosity was determined for the two samples using different plot methods taking Huggin’s plot as standard. The intrinsic viscosity was found to be 86.43 cm3/g and 64.59 cm3/g for acetylated and pure gum arabic respectively. Relative errors of other methods for the two samples was compared to that of Huggins and the plots that are most comparable to Huggins with relative errors less than 5% are; Martin, Lyon-Tobolsky, Staudinger-Heuer, Maron-Reznik and our proposed method. The proposed method which was a modification of the Kreiser method gave relative error less than 2 %, for both pure and modified gum. Whereas the Kreiser method gave relative error greater than 15 % for both methods. The critical concentration for the samples was found to be 0.0116 g/cm3 and 0.0155 g/cm3 for acetylated and pure gum respectively. This shows that there was no molecule-molecule entanglements during viscosity measurements.
{"title":"Comparative viscometric study of pure and acetylated gum Arabic using different plot methods","authors":"Alhassan S.I, Sule S.Y, M. R., R. H.I, Baso A.A, Sheshe F.A, Jafar S.C","doi":"10.14419/ijac.v8i1.30526","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30526","url":null,"abstract":"Acetylation of gum Arabic was achieved using acetic anhydride as solvent. The ester group formed was confirmed by FTIR spectra having absorption band of 750 cm-1 – 700 cm-1. Viscometric study of the pure and acetylated samples was carried out. Relative viscosity of acetylated gum was found to be higher than that of the pure gum. Intrinsic viscosity was determined for the two samples using different plot methods taking Huggin’s plot as standard. The intrinsic viscosity was found to be 86.43 cm3/g and 64.59 cm3/g for acetylated and pure gum arabic respectively. Relative errors of other methods for the two samples was compared to that of Huggins and the plots that are most comparable to Huggins with relative errors less than 5% are; Martin, Lyon-Tobolsky, Staudinger-Heuer, Maron-Reznik and our proposed method. The proposed method which was a modification of the Kreiser method gave relative error less than 2 %, for both pure and modified gum. Whereas the Kreiser method gave relative error greater than 15 % for both methods. The critical concentration for the samples was found to be 0.0116 g/cm3 and 0.0155 g/cm3 for acetylated and pure gum respectively. This shows that there was no molecule-molecule entanglements during viscosity measurements. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75825974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-06-04DOI: 10.14419/ijac.v8i1.30541
Ashok Kumar Sahoo, Sunil Kumar Pradhan, Ajaya Kumar Patnaik, S. Das
Molecular interaction studies using ultrasonic technique and spectroscopic investigation in ternary liquid mixture of drug 1,10-Phenanthroline, an α- amino acid Glycine and a transition metal salt like CuCl2 in aqueous medium has been carried out at 300.15K and 2 MHz frequency. Using experimental values of density (ρ),ultrasonic velocity (U) and UV analysis, different acoustical parameters such as adiabatic compressibility (β),intermolecular free length (Lf), acoustic impedance (z),relative association (RA),apparent molar volume (V∅), apparent molar adiabatic compressibility (K∅) are evaluated for the mixture of drug, glycine and inorganic salt CuCl2 at various concentrations. The ultrasonic and spectroscopic investigation clearly shows coordinative association of drug as a ligand with transition metal salt. Further interaction of glycine as an α- amino acid indicates a competitive association in the ternary system. The outcomes were expressed in terms of molecular interactions and the variation in parameters under varying solute concentrations providing conclusive remarks regarding the strength of intermolecular interactions in the ternary system with a bio-physical novelty.
{"title":"Study of ternary association of anti-microbial drug 1, 10-phenanthroline and α - amino acid with copper salt, an ultrasonic investigation of competitive interaction","authors":"Ashok Kumar Sahoo, Sunil Kumar Pradhan, Ajaya Kumar Patnaik, S. Das","doi":"10.14419/ijac.v8i1.30541","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30541","url":null,"abstract":"Molecular interaction studies using ultrasonic technique and spectroscopic investigation in ternary liquid mixture of drug 1,10-Phenanthroline, an α- amino acid Glycine and a transition metal salt like CuCl2 in aqueous medium has been carried out at 300.15K and 2 MHz frequency. Using experimental values of density (ρ),ultrasonic velocity (U) and UV analysis, different acoustical parameters such as adiabatic compressibility (β),intermolecular free length (Lf), acoustic impedance (z),relative association (RA),apparent molar volume (V∅), apparent molar adiabatic compressibility (K∅) are evaluated for the mixture of drug, glycine and inorganic salt CuCl2 at various concentrations. The ultrasonic and spectroscopic investigation clearly shows coordinative association of drug as a ligand with transition metal salt. Further interaction of glycine as an α- amino acid indicates a competitive association in the ternary system. The outcomes were expressed in terms of molecular interactions and the variation in parameters under varying solute concentrations providing conclusive remarks regarding the strength of intermolecular interactions in the ternary system with a bio-physical novelty. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81729108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-15DOI: 10.14419/ijac.v8i1.30513
D. N. Vidya, R. Chaithra, G. P. Senthil Kumar, D. R. Bhadresh
A simple, specific, quick, isocratic Reversed Phase High Performance Liquid Chromatographic method was developed and validated for the analysis of Pregabalin in bulk and 5-different pharmaceutical formulations, the separation was accomplished on a C18, 5μm Reverse Phase column (250 mm × 4.6 mm) using a methanol : water (95:5, v/v) mobile phase. The compound was eluted isocratically at a flow rate of 0.8 ml /min. The UV detector was set at 288 nm for the detection of Pregabalin (PRG). The method was linear over the range of 5-45 μg/ml and validated with respect to accuracy, precision, linearity, and specificity, limit of detection and limit of quantization. Robustness testing was also conducted to evaluate the effect of minor changes to the chromatographic system and to establish appropriate system suitability parameters. This method was used successfully for the quality assessment of 5-different pharmaceutical formulations with good precision and accuracy.
采用甲醇:水(95:5,v/v)流动相，采用C18, 5μm反相柱(250 mm × 4.6 mm)进行分离，建立了一种简单、特异、快速的反相高效液相色谱分析普瑞巴林原料药和5种不同制剂的方法。以0.8 ml /min的流速等量洗脱。采用分光光度为288 nm的紫外检测器检测普瑞巴林(PRG)。方法在5 ~ 45 μg/ml范围内呈线性，准确度、精密度、线性、特异性、检出限和定量限均得到验证。还进行了稳健性测试，以评估对色谱系统的微小变化的影响，并建立适当的系统适用性参数。该方法可用于5种不同制剂的质量评价，具有良好的精密度和准确度。
{"title":"Determination and validation of pregabalin in bulk and pharmaceutical formulations by reversed phase-high performance liquid chromatography","authors":"D. N. Vidya, R. Chaithra, G. P. Senthil Kumar, D. R. Bhadresh","doi":"10.14419/ijac.v8i1.30513","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30513","url":null,"abstract":"A simple, specific, quick, isocratic Reversed Phase High Performance Liquid Chromatographic method was developed and validated for the analysis of Pregabalin in bulk and 5-different pharmaceutical formulations, the separation was accomplished on a C18, 5μm Reverse Phase column (250 mm × 4.6 mm) using a methanol : water (95:5, v/v) mobile phase. The compound was eluted isocratically at a flow rate of 0.8 ml /min. The UV detector was set at 288 nm for the detection of Pregabalin (PRG). The method was linear over the range of 5-45 μg/ml and validated with respect to accuracy, precision, linearity, and specificity, limit of detection and limit of quantization. Robustness testing was also conducted to evaluate the effect of minor changes to the chromatographic system and to establish appropriate system suitability parameters. This method was used successfully for the quality assessment of 5-different pharmaceutical formulations with good precision and accuracy. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78738884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-15DOI: 10.14419/ijac.v8i1.30549
K. Bello
The present study is aimed at designing a pullulan based hydrogel through grafting technique for slow release of fluorouracil drug. To achieve this, pullulan grafted acrylic acid was synthesized and characterized by FTIR, and FESEM analyses. Swelling was done in different pH solutions with better swelling in pH 2. As such, the drug loading was done in the specified pH environment. The release profile revealed that more than 90 % of the drug could be released within 5 days. The prepared hydrogel may be considered as stimuli responsive materials for oral drug delivery of anti-cancer drugs.
{"title":"Synthesis and characterization of pullulan derived hydrogel matrix as carrier for slow release of anti-cancer drug","authors":"K. Bello","doi":"10.14419/ijac.v8i1.30549","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30549","url":null,"abstract":"The present study is aimed at designing a pullulan based hydrogel through grafting technique for slow release of fluorouracil drug. To achieve this, pullulan grafted acrylic acid was synthesized and characterized by FTIR, and FESEM analyses. Swelling was done in different pH solutions with better swelling in pH 2. As such, the drug loading was done in the specified pH environment. The release profile revealed that more than 90 % of the drug could be released within 5 days. The prepared hydrogel may be considered as stimuli responsive materials for oral drug delivery of anti-cancer drugs. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78969643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-15DOI: 10.14419/ijac.v8i1.30542
Likitha T G, J. Gnana Babu C., S. H G
A rapid, simple, precise and accurate high performance thin layer chromatographic method has been developed and validated for the estima-tion of Levetiracetam in bulk and tablet dosage form. The Levetiracetam was chromatographed on silica gel 60 F254 HPTLC plate as a sta-tionary phase. The mobile phase was Ethyl acetate: Methanol: Ammonia in the ratio of 7:1:2 respectively. It gave a dense and compact spot of Levetiracetam with an Rf value of 0.56. The quantitation was carried out at 210 nm. The method was validated in terms of linearity, accu-racy, precision and specificity. The statistical analysis proved that the developed method is accurate and reproducible with linearity in the range of 100 to 500 ng/spot. The limit of detection and limit of quantitation for Levetiracetam were 3.55 and 10.66 ng/spot respectively. The developed method can be employed for the routine analysis of Levetiracetam in the tablet dosage form.
{"title":"Validated HPTLC method for the quantitation of levetiracetam in pure and tablet dosage form","authors":"Likitha T G, J. Gnana Babu C., S. H G","doi":"10.14419/ijac.v8i1.30542","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30542","url":null,"abstract":"A rapid, simple, precise and accurate high performance thin layer chromatographic method has been developed and validated for the estima-tion of Levetiracetam in bulk and tablet dosage form. The Levetiracetam was chromatographed on silica gel 60 F254 HPTLC plate as a sta-tionary phase. The mobile phase was Ethyl acetate: Methanol: Ammonia in the ratio of 7:1:2 respectively. It gave a dense and compact spot of Levetiracetam with an Rf value of 0.56. The quantitation was carried out at 210 nm. The method was validated in terms of linearity, accu-racy, precision and specificity. The statistical analysis proved that the developed method is accurate and reproducible with linearity in the range of 100 to 500 ng/spot. The limit of detection and limit of quantitation for Levetiracetam were 3.55 and 10.66 ng/spot respectively. The developed method can be employed for the routine analysis of Levetiracetam in the tablet dosage form.","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90704034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-28DOI: 10.14419/ijac.v8i1.30498
C. R., Senthil Kumar Gp, P. P, Vidya Dn, Bhadresh Dr, M. S
In the present research a simple, accurate, precise and cost-effective High-performance thin layer chromatographic method for the estimation of clobazam, in bulk and pharmaceutical dosage form was illustrated. The RF value of the drug was found to be 0.74 in the mobile phase, acetone: toluene: formic acid (1: 1: 0.05 v/v/v). A linear response was observed in the range of 100-700 ng with a regression coefficient of 0.999. Validation parameters were carried out as per the guidelines of International Conference for Harmonization (ICH). This method can be used in the industries for determination of clobazam to analyze the quality of formulation without interference of the excipients.
{"title":"Method development and validation of clobazam in bulk and pharmaceutical dosage forms by using high performance thin layer chromatographic method","authors":"C. R., Senthil Kumar Gp, P. P, Vidya Dn, Bhadresh Dr, M. S","doi":"10.14419/ijac.v8i1.30498","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30498","url":null,"abstract":"In the present research a simple, accurate, precise and cost-effective High-performance thin layer chromatographic method for the estimation of clobazam, in bulk and pharmaceutical dosage form was illustrated. The RF value of the drug was found to be 0.74 in the mobile phase, acetone: toluene: formic acid (1: 1: 0.05 v/v/v). A linear response was observed in the range of 100-700 ng with a regression coefficient of 0.999. Validation parameters were carried out as per the guidelines of International Conference for Harmonization (ICH). This method can be used in the industries for determination of clobazam to analyze the quality of formulation without interference of the excipients. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"142 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77952074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-28DOI: 10.14419/ijac.v8i1.30607
Alireza Heidari, Katrin E. Schmitt, M. Henderson, E. Besana
Cancer is one of the malignant diseases and millions of people worldwide die from cancer annually. Breast cancer diagnosis requires the analysis of images and attributes as well as collecting many clinical and mammography variables. In diagnosis of breast cancer, it is im-portant to determine whether a tumor is benign or malignant. The information about breast cancer risk prediction along with the type of tu-mor are crucial for patients and effective medical decision making. An ideal diagnostic system could effectively distinguish between benign and malignant cells; however, such a system has not been created yet. In this study, a model is developed to improve the prediction probabil-ity of breast cancer. It is necessary to have such a prediction model as the survival probability of breast cancer is high when patients are diagnosed at early stages.
{"title":"Hereditary immunity in cancer","authors":"Alireza Heidari, Katrin E. Schmitt, M. Henderson, E. Besana","doi":"10.14419/ijac.v8i1.30607","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30607","url":null,"abstract":"Cancer is one of the malignant diseases and millions of people worldwide die from cancer annually. Breast cancer diagnosis requires the analysis of images and attributes as well as collecting many clinical and mammography variables. In diagnosis of breast cancer, it is im-portant to determine whether a tumor is benign or malignant. The information about breast cancer risk prediction along with the type of tu-mor are crucial for patients and effective medical decision making. An ideal diagnostic system could effectively distinguish between benign and malignant cells; however, such a system has not been created yet. In this study, a model is developed to improve the prediction probabil-ity of breast cancer. It is necessary to have such a prediction model as the survival probability of breast cancer is high when patients are diagnosed at early stages. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78050926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-18DOI: 10.14419/ijac.v8i1.30490
Alireza Heidari, Katrin E. Schmitt, M. Henderson, E. Besana
Cancer is the most popular reason of death worldwide that many people struggle with it. Although the cancer is dangerous, but if it detects in early stages increases the chance of patient survival. The miRNAs are one of the important ways for early cancer detection that it caused to return an interesting field for researches. All the miRNAs haven’t any role in cancer detection. The Quantum Genetic Algorithm (QGA) is a developed Genetic Algorithm (GA) that by using of quantum computing on top of the genetic algorithm to alleviate the pre convergence problem. The interest of this study is to adopt the QGA for solving of informative miRNAs selection and irrelevant miRNAs removing problem. However, in the suggested algorithm, SVM classifier performance and the dimension of the selected feature vector are dependent on heuristic information for QGA. As a result, the proposed approach selects the adaptive feature subset with respect to the shortest feature dimension and the improved performance of the classifier. The performances of this method are evaluated on the popular data set which the experimental results show that since QGA-SVM is used as one of wrapper methods, as a result, its overall performance is better separation between normal and cancer expression for all types of cancer and better classification rate.
{"title":"A chemical review on cancer immunology and immunodeficiency","authors":"Alireza Heidari, Katrin E. Schmitt, M. Henderson, E. Besana","doi":"10.14419/ijac.v8i1.30490","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30490","url":null,"abstract":"Cancer is the most popular reason of death worldwide that many people struggle with it. Although the cancer is dangerous, but if it detects in early stages increases the chance of patient survival. The miRNAs are one of the important ways for early cancer detection that it caused to return an interesting field for researches. All the miRNAs haven’t any role in cancer detection. The Quantum Genetic Algorithm (QGA) is a developed Genetic Algorithm (GA) that by using of quantum computing on top of the genetic algorithm to alleviate the pre convergence problem. The interest of this study is to adopt the QGA for solving of informative miRNAs selection and irrelevant miRNAs removing problem. However, in the suggested algorithm, SVM classifier performance and the dimension of the selected feature vector are dependent on heuristic information for QGA. As a result, the proposed approach selects the adaptive feature subset with respect to the shortest feature dimension and the improved performance of the classifier. The performances of this method are evaluated on the popular data set which the experimental results show that since QGA-SVM is used as one of wrapper methods, as a result, its overall performance is better separation between normal and cancer expression for all types of cancer and better classification rate. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85053827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-04-18DOI: 10.14419/ijac.v8i1.30500
Alireza Heidari, Victoria Peterson
In this research, poly (D, L-lactide-co-glycolide)-block-poly (ethylene glycol), (PLGA-PEG) nanoparticles (NPs) of less than 195 nm in diameter containing of Naringenin (NRG) a naturally flavonoid were synthesized. Encapsulated form NRG improves its medical properties and solubility. The therapeutic efficacy of the encapsulated naringenin (NRG-NPs) and NRG on human lung epithelial (A549) and mouse mammary (4T1) carcinoma cells proliferation was determined by MTT assays. The cytotoxicity potency was rated as follows: NRG-NPs > NRG. The antioxidant effects of the NRG and NRG-NPs were also determined by FRAP method. Our results show that NRG-NPs are cytotoxic compounds for cancer cells and anti-cancer effect can be attributed to the presence of Fe chelatory and antioxidant effects of NRG-NPs.
本研究合成了含天然类黄酮柚皮素(NRG)的直径小于195 nm的聚(D, l -丙交酯-共乙醇酸酯)-嵌段聚乙二醇(PLGA-PEG)纳米颗粒(NPs)。包封后的NRG改善了其医用性能和溶解性。MTT法观察柚皮素包被物(NRG- nps)和NRG对人肺上皮细胞(A549)和小鼠乳腺(4T1)癌细胞增殖的影响。细胞毒效价分级如下:NRG- nps > NRG。采用FRAP法测定NRG和NRG- nps的抗氧化作用。我们的研究结果表明，NRG-NPs是癌细胞的细胞毒性化合物，其抗癌作用可能归因于NRG-NPs的铁螯合和抗氧化作用。
{"title":"A comprehensive review on functional roles of cancerous immunoglobulins and potential applications in cancer immunodiagnostics and immunotherapy","authors":"Alireza Heidari, Victoria Peterson","doi":"10.14419/ijac.v8i1.30500","DOIUrl":"https://doi.org/10.14419/ijac.v8i1.30500","url":null,"abstract":"In this research, poly (D, L-lactide-co-glycolide)-block-poly (ethylene glycol), (PLGA-PEG) nanoparticles (NPs) of less than 195 nm in diameter containing of Naringenin (NRG) a naturally flavonoid were synthesized. Encapsulated form NRG improves its medical properties and solubility. The therapeutic efficacy of the encapsulated naringenin (NRG-NPs) and NRG on human lung epithelial (A549) and mouse mammary (4T1) carcinoma cells proliferation was determined by MTT assays. The cytotoxicity potency was rated as follows: NRG-NPs > NRG. The antioxidant effects of the NRG and NRG-NPs were also determined by FRAP method. Our results show that NRG-NPs are cytotoxic compounds for cancer cells and anti-cancer effect can be attributed to the presence of Fe chelatory and antioxidant effects of NRG-NPs. ","PeriodicalId":13723,"journal":{"name":"International Journal of Advanced Chemistry","volume":"88 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74593231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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