Pub Date : 2024-07-10DOI: 10.47552/ijam.v15i2.4593
Dhanashri Joil, S. Tavhare, Avinash Sanap, Ramesh Bhonde
Obesity can be described as the "New World Syndrome". It is a multi-factorial disorder, which is often associated with many other significant diseases such as diabetes, hypertension and other cardiovascular diseases, osteoarthritis and certain cancers. The management of obesity will therefore require a comprehensive range of strategies focussing on existing weight problems and obesity complications. Herbal drugs are an integral part of the traditional medicines due to presence of wide range of nature made bioactive compounds. Musta (Cyperus rotundus L.) is one such drug practiced for management of obesity in Ayurveda and other traditional system of medicines. The present study reports the cytotoxicity and anti-adipogenic effect of aqueous and ethanolic extracts of C. rotundus in 3T3L1 cell line at concentration 1, 10 and 100 μg /ml. MTT (3-(4,5-dimetrhylthiazole-2-yl)-2,5-diphenyl tetrazolium) assay revealed non-toxic nature of both extracts. Inhibition of lipid accumulation in cells was observed significant in ethanolic extract. Significant reduction in lipid accumulation at 100 μg /ml concentration was observed in ethanol extract sample. The anti-adipogenic effect of the Musta is attributed to the reduced expression of PPARg (Peroxisome proliferator-activated receptor gamma), and increased expression of GLUT4 (Glucose transporter protein type-4) in 3T3 L1 cells as assessed by the RT-PCR. The Cyperus rotundus L. in ethanolic extract proves a potential herb for the management of obesity and related non-communicable diseases.
{"title":"Anti- adipogenic actions of Cyperus rotundus L. in 3T3L-1 Cells","authors":"Dhanashri Joil, S. Tavhare, Avinash Sanap, Ramesh Bhonde","doi":"10.47552/ijam.v15i2.4593","DOIUrl":"https://doi.org/10.47552/ijam.v15i2.4593","url":null,"abstract":"Obesity can be described as the \"New World Syndrome\". It is a multi-factorial disorder, which is often associated with many other significant diseases such as diabetes, hypertension and other cardiovascular diseases, osteoarthritis and certain cancers. The management of obesity will therefore require a comprehensive range of strategies focussing on existing weight problems and obesity complications. Herbal drugs are an integral part of the traditional medicines due to presence of wide range of nature made bioactive compounds. Musta (Cyperus rotundus L.) is one such drug practiced for management of obesity in Ayurveda and other traditional system of medicines. The present study reports the cytotoxicity and anti-adipogenic effect of aqueous and ethanolic extracts of C. rotundus in 3T3L1 cell line at concentration 1, 10 and 100 μg /ml. MTT (3-(4,5-dimetrhylthiazole-2-yl)-2,5-diphenyl tetrazolium) assay revealed non-toxic nature of both extracts. Inhibition of lipid accumulation in cells was observed significant in ethanolic extract. Significant reduction in lipid accumulation at 100 μg /ml concentration was observed in ethanol extract sample. The anti-adipogenic effect of the Musta is attributed to the reduced expression of PPARg (Peroxisome proliferator-activated receptor gamma), and increased expression of GLUT4 (Glucose transporter protein type-4) in 3T3 L1 cells as assessed by the RT-PCR. The Cyperus rotundus L. in ethanolic extract proves a potential herb for the management of obesity and related non-communicable diseases. ","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141661899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-07-10DOI: 10.47552/ijam.v15i2.4651
Tamali Halder, Sameer Gupta, Chandana Haldar, Parimal Das
Ayurveda is a traditional medication system in India. Experimental validation of ayurvedic formulations to combat with progressive, non-curable diseases like cancer, neurodegenerative disorders and knowing their mode of action are of current interest. The present study was aimed to explore the effect of dietary supplement of Rasa-sindoor (RS), an organo-metallic derivative of mercury, on drug (MPTP: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine) induced mouse model of Parkinson disease (PD). For this, mice were divided into three groups: (1) vehicle control (Normal saline treated), (2) Treated 1 (MPTP treated: 15mg/kg body weight, twice a day in 2 hours interval for 2 days, intraperitoneal injection) and (3) Treated 2 (MPTP: same as Treated 1 and Rasa Sindoor: 1gm/kg RS twice a day for 4 days). Total cellular RNA was extracted from sacrificed mice brain tissue and Immunohistochemistry (IHC) was performed for Tyrosine Hydroxylase (TH) in 100 µ section of mid-brain region to check dopaminergic neurodegeneration. IHC revealed reduced TH activity in Treated 1 and Treated 2 as compared to vehicle control while Treated 2 showed greater TH activity than that of Treated 1. This preliminary observation was followed by a microarray based gene expression analysis, which results differential expression of a new set of genes involving neurogenesis, growth cone formation and axon guidance, activation of latent precursor cells etc.; validated by semi-quantitative real time PCR. Our result suggests that RS is neuroprotective and its mode of action may be through prevention of cell apoptosis and activation of latent precursor cells and cell growth.
{"title":"The effect of an Indian traditional Ayurvedic formulation (Rasa-sindoor) on Parkinson disease mouse model","authors":"Tamali Halder, Sameer Gupta, Chandana Haldar, Parimal Das","doi":"10.47552/ijam.v15i2.4651","DOIUrl":"https://doi.org/10.47552/ijam.v15i2.4651","url":null,"abstract":"Ayurveda is a traditional medication system in India. Experimental validation of ayurvedic formulations to combat with progressive, non-curable diseases like cancer, neurodegenerative disorders and knowing their mode of action are of current interest. The present study was aimed to explore the effect of dietary supplement of Rasa-sindoor (RS), an organo-metallic derivative of mercury, on drug (MPTP: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine) induced mouse model of Parkinson disease (PD). For this, mice were divided into three groups: (1) vehicle control (Normal saline treated), (2) Treated 1 (MPTP treated: 15mg/kg body weight, twice a day in 2 hours interval for 2 days, intraperitoneal injection) and (3) Treated 2 (MPTP: same as Treated 1 and Rasa Sindoor: 1gm/kg RS twice a day for 4 days). Total cellular RNA was extracted from sacrificed mice brain tissue and Immunohistochemistry (IHC) was performed for Tyrosine Hydroxylase (TH) in 100 µ section of mid-brain region to check dopaminergic neurodegeneration. IHC revealed reduced TH activity in Treated 1 and Treated 2 as compared to vehicle control while Treated 2 showed greater TH activity than that of Treated 1. This preliminary observation was followed by a microarray based gene expression analysis, which results differential expression of a new set of genes involving neurogenesis, growth cone formation and axon guidance, activation of latent precursor cells etc.; validated by semi-quantitative real time PCR. Our result suggests that RS is neuroprotective and its mode of action may be through prevention of cell apoptosis and activation of latent precursor cells and cell growth.","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141661590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Acalypha indica Linn.(AI) is a herbaceous annual catkin-like inflorescences belonging to the Euphorbiaceae family. AI has a wide range of therapeutic effects including laxative properties, anthelmintic, wound healing, antiseptic etc. Objectives: This study was to carry out the comparative standardisation study for a siddha source herb Acalypha indica(Linn) aerial parts and marketed raw material by collection and authentication of aerial parts of AI and to evaluate the physicochemical constants such as LOD, ash values, extractive values as per the standard methods. Materials and Methods: The preparation of hydro alcoholic and ethyl acetate extract was carried out by cold maceration extraction procedure for both fresh leaves and raw material of AI. Results: The preliminary physicochemical screening tests, quantitative determination of total flavonoid and phenolic contents, hydrogel preparation of AI was carried out. The HPTLC finger printing for ethyl acetate extract of fresh leaf was developed in comparison with three standards Quercetin, Gallic acid and Beta sitosterol and were scanned under UV light at 397nm, 280nm, 580nm respectively. The number of peaks obtained in AI extract were observed to be 3, 8 and 28 respectively. Pesticide analysis regulations often require chemical testing using confirmatory techniques such as GC/MS and about 26 compounds were identified under suitable gas chromatographic conditions. Conclusion: The presence of phytoconstituents were analyzed and the studies were performed to aid further investigations for the identification of therapeutic value.
背景介绍Acalypha indica Linn.（AI）是一种一年生柔荑花序状草本植物，属于大戟科。AI具有广泛的治疗作用，包括润肠通便、驱虫、伤口愈合、防腐等。研究目的本研究旨在通过采集和鉴定 AI 的气生部分，对一种释迦源草药 Acalypha indica（Linn）的气生部分和市场上销售的原材料进行比较标准化研究，并根据标准方法对 LOD、灰分值、萃取值等理化常数进行评估。材料和方法：采用冷浸渍萃取法对人工湿地的新鲜叶片和原材料进行水醇和乙酸乙酯萃取。结果：进行了初步的理化筛选试验、总黄酮和酚含量的定量测定以及人工合成植物水凝胶的制备。将新鲜叶片的乙酸乙酯提取物与三种标准物质槲皮素、没食子酸和β-谷甾醇进行对比，并在紫外光下分别于 397nm、280nm 和 580nm 处进行扫描。在 AI 提取物中观察到的峰数分别为 3、8 和 28。农药分析法规通常要求使用气相色谱/质谱等确证技术进行化学测试，在合适的气相色谱条件下，鉴定出了约 26 种化合物。结论对植物成分的存在进行了分析和研究，以帮助进一步调查确定其治疗价值。
{"title":"Comparative Standardisation Study of a Siddha Source Herb Acalypha indica Linn. Aerial Parts and Marketed Raw Material - An Insight to Develop Monograph","authors":"Teshini S, Swetha T, Mangathayaru K, Husna Kanswal Qureshi, Vinodhini C","doi":"10.47552/ijam.v15i2.4093","DOIUrl":"https://doi.org/10.47552/ijam.v15i2.4093","url":null,"abstract":"Background: Acalypha indica Linn.(AI) is a herbaceous annual catkin-like inflorescences belonging to the Euphorbiaceae family. AI has a wide range of therapeutic effects including laxative properties, anthelmintic, wound healing, antiseptic etc. Objectives: This study was to carry out the comparative standardisation study for a siddha source herb Acalypha indica(Linn) aerial parts and marketed raw material by collection and authentication of aerial parts of AI and to evaluate the physicochemical constants such as LOD, ash values, extractive values as per the standard methods. Materials and Methods: The preparation of hydro alcoholic and ethyl acetate extract was carried out by cold maceration extraction procedure for both fresh leaves and raw material of AI. Results: The preliminary physicochemical screening tests, quantitative determination of total flavonoid and phenolic contents, hydrogel preparation of AI was carried out. The HPTLC finger printing for ethyl acetate extract of fresh leaf was developed in comparison with three standards Quercetin, Gallic acid and Beta sitosterol and were scanned under UV light at 397nm, 280nm, 580nm respectively. The number of peaks obtained in AI extract were observed to be 3, 8 and 28 respectively. Pesticide analysis regulations often require chemical testing using confirmatory techniques such as GC/MS and about 26 compounds were identified under suitable gas chromatographic conditions. Conclusion: The presence of phytoconstituents were analyzed and the studies were performed to aid further investigations for the identification of therapeutic value.","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141661735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-24DOI: 10.47552/ijam.v15i1.3745
Siva Lakshmi S, Vetha Merlin Kumari H, Nalina Sofia H, L. T, M. R
Abstract: Background: Kumaara veeriya kaantha chenduram (KVKC) is a siddha herbomineral formulation indicated for paandunoi in Siddha literature (1).Paandu noi is a disease characterized by changes in the body's natural color andpallor found in the skin, nails and conjunctiva. Objectives: To determine the efficacy of the KVKC, the open labed clinical study was conducted in National Institute of Siddha OPD. Materials and Methods: The 30 patients with Iron deficiency anaemiawere selected and treated with KVKC orally with the dose of 260 milli gram twice in a day with Nellikailegiyam6 gram continuously for 45 days. Laboratory investigations for hemoglobin was done on 1st day and every 15 days once of the study. Results: Clinical assessment was done, based on the before and after treatment scores which was compared statistically and recorded.Regarding Hemoglobin level, 24(80%) patients showed increase of 1 to 3 grams from base level, 6(20%) patients showed increase of 0.1 to 0.9 grams from base level.Regarding Serum Ferritin, out of 30 patients, 9 (30%) patients showed 0.1-3.5 ng/ml increase from its base level, 21 (70%) patients showed 3.6-7.0 ng/ml increase from its base level.From the Statistical studies, the Mean ± Standard deviation for Haemoglobin before treatment was 9.7 ± 0.93 and after treatment was 11.18 ± 1.08 which was statistically highly significant (p<0.0001).And Serum ferritin before treatment was 9.27 ± 3.31, after treatment was 13.5 ± 3.49 which was statistically significant (p<0.0001).
{"title":"Clinical evaluation of therapeutic efficacy of a Siddha herbo mineral formulation “Kumaara Veeriya Kaantha Chenduram’’ with “Nellikkai Legiyam” (internal) in the treatment of Iron Deficiency Anemia","authors":"Siva Lakshmi S, Vetha Merlin Kumari H, Nalina Sofia H, L. T, M. R","doi":"10.47552/ijam.v15i1.3745","DOIUrl":"https://doi.org/10.47552/ijam.v15i1.3745","url":null,"abstract":"Abstract:\u0000Background:\u0000Kumaara veeriya kaantha chenduram (KVKC) is a siddha herbomineral formulation indicated for paandunoi in Siddha literature (1).Paandu noi is a disease characterized by changes in the body's natural color andpallor found in the skin, nails and conjunctiva.\u0000Objectives:\u0000To determine the efficacy of the KVKC, the open labed clinical study was conducted in National Institute of Siddha OPD.\u0000Materials and Methods:\u0000The 30 patients with Iron deficiency anaemiawere selected and treated with KVKC orally with the dose of 260 milli gram twice in a day with Nellikailegiyam6 gram continuously for 45 days. Laboratory investigations for hemoglobin was done on 1st day and every 15 days once of the study.\u0000 \u0000 \u0000Results:\u0000Clinical assessment was done, based on the before and after treatment scores which was compared statistically and recorded.Regarding Hemoglobin level, 24(80%) patients showed increase of 1 to 3 grams from base level, 6(20%) patients showed increase of 0.1 to 0.9 grams from base level.Regarding Serum Ferritin, out of 30 patients, 9 (30%) patients showed 0.1-3.5 ng/ml increase from its base level, 21 (70%) patients showed 3.6-7.0 ng/ml increase from its base level.From the Statistical studies, the Mean ± Standard deviation for Haemoglobin before treatment was 9.7 ± 0.93 and after treatment was 11.18 ± 1.08 which was statistically highly significant (p<0.0001).And Serum ferritin before treatment was 9.27 ± 3.31, after treatment was 13.5 ± 3.49 which was statistically significant (p<0.0001).","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140659502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.47552/ijam.v15i1.4261
Ilakkiya B Gandhi, Meenakshi Sundaram M
Cancer is an uncontrolled proliferation of cells that can affect nearby tissues as well as distant organs. Cervical cancer is predicted to be among the four most prevalent cancers in women overall in 2020, with 342,000 mortality and 604,000 new cases. Around 6-29% of all cancers in women in India are caused by cervical cancer. Women who have had several sexual partners, recurrent abortions, or vaginal deliveries—all of which cause repetitive stress to the cervix are more likely to develop cervical cancer. Radiation, chemotherapy, and surgery are being used to treat cancer. All of these treatments come with side effects, which can range in severity and have a serious psychological impact on patients. The Siddha system has a number of accessible herbo mineral formulations for treating cervical cancer. According to Siddha literature (Agasthiyar Vaidhya Rathina Churukkam, 360), the treatment of cervical cancer (yoni puttru) is advised for the Siddha medicine Vallarai Kirutham with Rasa parpam. The goal of this research was to find out this formulation's potential anti-cancer properties using MTT assays on HeLa cell lines. These findings of the current study suggest that the MTT technique's lowest reading for cell viability was 1.26±0.009% at a concentration of 100 µl/ml. This was followed by concentrations of 75 µl/ml, 50µl/ml and 25µl/ml which showed 7.91±0.004%, 23.91 ±0.01% and 37.17± 0.01% similarly, 10 µl/ml shows 52.09±0.01%.The matching IC50 value was discovered to be 12.44% and AO/EB dual staining was used to examine apoptotic activity. The outcomes of in-vitro experiments using the HeLa cell line. According to the outcome of in vitro experiments done on the HeLa cell line, the drug had substantial anti-cancer and therapeutic value in the management of cervical tumours .With a variety of time tested medications the Siddha system has shown outcomes that were clinically important for the management of cervical carcinoma. One of those medications has to undergo additional testing in patients with cervical cancer through clinical research.
癌症是一种不受控制的细胞增殖，可影响邻近组织和远处器官。预计到 2020 年，宫颈癌将成为女性四大高发癌症之一，死亡人数将达到 34.2 万，新增病例将达到 60.4 万。在印度，大约 6-29% 的女性癌症是由宫颈癌引起的。有多个性伴侣、反复人工流产或阴道分娩的妇女--所有这些都会对宫颈造成反复压力--更容易患上宫颈癌。目前正在使用放射治疗、化学治疗和手术治疗癌症。所有这些治疗方法都会产生副作用，其严重程度不一，并对患者的心理造成严重影响。释迦系统有许多可用于治疗宫颈癌的herbo矿物配方。根据悉达文献（Agasthiyar Vaidhya Rathina Churukkam, 360），治疗宫颈癌（yoni puttru）建议使用悉达药物 Vallarai Kirutham 和 Rasa parpam。本研究的目的是通过对 HeLa 细胞系进行 MTT 检测，找出这种配方的潜在抗癌特性。目前的研究结果表明，在浓度为 100 µl/ml 时，MTT 技术的细胞存活率最低读数为 1.26±0.009%。相匹配的 IC50 值为 12.44%，AO/EB 双染色用于检测细胞凋亡活性。使用 HeLa 细胞系进行体外实验的结果。根据在 HeLa 细胞系上进行的体外实验结果，该药物在治疗宫颈肿瘤方面具有很高的抗癌和治疗价值。其中一种药物必须通过临床研究在宫颈癌患者中进行更多测试。
{"title":"In vitro cytotoxicity and apoptosis inducing effect of Vallarai Kirutham with Rasa Parpam in HeLa Cell Lines","authors":"Ilakkiya B Gandhi, Meenakshi Sundaram M","doi":"10.47552/ijam.v15i1.4261","DOIUrl":"https://doi.org/10.47552/ijam.v15i1.4261","url":null,"abstract":"Cancer is an uncontrolled proliferation of cells that can affect nearby tissues as well as distant organs. Cervical cancer is predicted to be among the four most prevalent cancers in women overall in 2020, with 342,000 mortality and 604,000 new cases. Around 6-29% of all cancers in women in India are caused by cervical cancer. Women who have had several sexual partners, recurrent abortions, or vaginal deliveries—all of which cause repetitive stress to the cervix are more likely to develop cervical cancer. Radiation, chemotherapy, and surgery are being used to treat cancer. All of these treatments come with side effects, which can range in severity and have a serious psychological impact on patients. The Siddha system has a number of accessible herbo mineral formulations for treating cervical cancer. According to Siddha literature (Agasthiyar Vaidhya Rathina Churukkam, 360), the treatment of cervical cancer (yoni puttru) is advised for the Siddha medicine Vallarai Kirutham with Rasa parpam. The goal of this research was to find out this formulation's potential anti-cancer properties using MTT assays on HeLa cell lines. These findings of the current study suggest that the MTT technique's lowest reading for cell viability was 1.26±0.009% at a concentration of 100 µl/ml. This was followed by concentrations of 75 µl/ml, 50µl/ml and 25µl/ml which showed 7.91±0.004%, 23.91 ±0.01% and 37.17± 0.01% similarly, 10 µl/ml shows 52.09±0.01%.The matching IC50 value was discovered to be 12.44% and AO/EB dual staining was used to examine apoptotic activity. The outcomes of in-vitro experiments using the HeLa cell line. According to the outcome of in vitro experiments done on the HeLa cell line, the drug had substantial anti-cancer and therapeutic value in the management of cervical tumours .With a variety of time tested medications the Siddha system has shown outcomes that were clinically important for the management of cervical carcinoma. One of those medications has to undergo additional testing in patients with cervical cancer through clinical research.","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140775441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.47552/ijam.v15i1.4188
Santosh Marennavar, Sakshi Shahapurmath, Shafin Paneri, Tanvi Nayak, Akshay K Patil, S. Jalalpure
Aim: To incorporate standardized Curcuma longa Linn extract into phytosomes and evaluate for in vitro anti-inflammatory and BSL bioassay. Method: The quality of the plant material was determined by various pharmacognostic parameters. The plant material was then subjected to maceration for extraction using ethanol: water as solvent followed by Soxhlet extraction. The resulting extract was subjected to phytochemical analysis to determine the presence of plant metabolites. The drug and excipients compatibility was evaluated by FTIR study. Furthermore, using the thin film hydration approach, a new lipid-based phytosome was prepared. In vitro anti-inflammatory and brine shrimp lethality tests were performed on prepared phytosome. Results: Moisture content, total ash, acid insoluble ash, water soluble ash values, aqueous, alcohol, and petroleum ether extractive values are all found to be within limits. The phytochemical analysis validated the existence of alkaloids, tannins, resins, carbohydrates, proteins, flavonoids, and saponins. The compatibility study demonstrates the compatibility of excipients with drugs. Thin film hydration technique was employed successfully to prepare the phytosomes containing Curcuma longa linn extract. In vitro anti-inflammatory activity revealed that prepared phytosome could serve as natural based therapeutic option for anti-inflammatory potential. Brine shrimp lethality assay also confirmed the bioactivity of prepared phytosomes. Conclusion: The method used for standardization can be used to aid with plant identification and quality analysis of Curcuma longa Linn for future research. It can be inferred from the findings that phytosomes loaded with Curcuma longa Linn extract exhibited promising anti-inflammatory and cytotoxic effects.
{"title":"Incorporation of standardised extract of Curcuma longa Linn into phytosomes and its evaluation for in vitro Anti-inflammatory potential and Brine shrimp lethality assay","authors":"Santosh Marennavar, Sakshi Shahapurmath, Shafin Paneri, Tanvi Nayak, Akshay K Patil, S. Jalalpure","doi":"10.47552/ijam.v15i1.4188","DOIUrl":"https://doi.org/10.47552/ijam.v15i1.4188","url":null,"abstract":"Aim: To incorporate standardized Curcuma longa Linn extract into phytosomes and evaluate for in vitro anti-inflammatory and BSL bioassay. Method: The quality of the plant material was determined by various pharmacognostic parameters. The plant material was then subjected to maceration for extraction using ethanol: water as solvent followed by Soxhlet extraction. The resulting extract was subjected to phytochemical analysis to determine the presence of plant metabolites. The drug and excipients compatibility was evaluated by FTIR study. Furthermore, using the thin film hydration approach, a new lipid-based phytosome was prepared. In vitro anti-inflammatory and brine shrimp lethality tests were performed on prepared phytosome. Results: Moisture content, total ash, acid insoluble ash, water soluble ash values, aqueous, alcohol, and petroleum ether extractive values are all found to be within limits. The phytochemical analysis validated the existence of alkaloids, tannins, resins, carbohydrates, proteins, flavonoids, and saponins. The compatibility study demonstrates the compatibility of excipients with drugs. Thin film hydration technique was employed successfully to prepare the phytosomes containing Curcuma longa linn extract. In vitro anti-inflammatory activity revealed that prepared phytosome could serve as natural based therapeutic option for anti-inflammatory potential. Brine shrimp lethality assay also confirmed the bioactivity of prepared phytosomes. Conclusion: The method used for standardization can be used to aid with plant identification and quality analysis of Curcuma longa Linn for future research. It can be inferred from the findings that phytosomes loaded with Curcuma longa Linn extract exhibited promising anti-inflammatory and cytotoxic effects.","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140757699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.47552/ijam.v15i1.4315
Bhagyashree Jibkate, B. Rathi, A. Wanjari, Akshay Pargaonkar
Medicated oils are used for external as well as internal administration to treat various disorders. In Ayurvedic literature Murchhana is a procedure carried out on crude oil as treatment to enhance properties of oil. In this study, Chincha Lavan Taila a medicated formulation is prepared by two procedures Murchhita Chincha Lavan Taila (MCLT) and Amurchhita Chincha Lavan Taila (ACLT). This study aims to assess the in-vitro diffusion of Chincha Lavan Taila formulated by processed sesame oil and crude sesame oil. The in-vitro rate and extent of permeability of drug dosage forms are good markers to access the absorbance of the drug. The objective of this study is to evaluate the drug release of both oils by using in- vitro absorbance methodology of Franz diffusion cell apparatus. The in- vitro absorbance was studied in Franz diffusion cell apparatus at pH 3 and pH 8. The samples were collected and analysed under UV spectrophotometer to note absorbance of oil at different wavelength. It was noted that in the buffer solution of pH 8, there was maximum absorbance of all the samples. This experimental study shows that both formulations have well sustained absorption through gastrointestinal tract. The results indicate that the rate of absorption exhibited to be higher in MCLT compared to ACLT. This work helps to develop a new method to evaluate intestinal absorption and compare formulation for therapeutic efficacy and drug absorbance.
{"title":"Assessment of in vitro diffusion of Chincha Lavan Taila: An Ayurveda medicated oil","authors":"Bhagyashree Jibkate, B. Rathi, A. Wanjari, Akshay Pargaonkar","doi":"10.47552/ijam.v15i1.4315","DOIUrl":"https://doi.org/10.47552/ijam.v15i1.4315","url":null,"abstract":"Medicated oils are used for external as well as internal administration to treat various disorders. In Ayurvedic literature Murchhana is a procedure carried out on crude oil as treatment to enhance properties of oil. In this study, Chincha Lavan Taila a medicated formulation is prepared by two procedures Murchhita Chincha Lavan Taila (MCLT) and Amurchhita Chincha Lavan Taila (ACLT). This study aims to assess the in-vitro diffusion of Chincha Lavan Taila formulated by processed sesame oil and crude sesame oil. The in-vitro rate and extent of permeability of drug dosage forms are good markers to access the absorbance of the drug. The objective of this study is to evaluate the drug release of both oils by using in- vitro absorbance methodology of Franz diffusion cell apparatus. The in- vitro absorbance was studied in Franz diffusion cell apparatus at pH 3 and pH 8. The samples were collected and analysed under UV spectrophotometer to note absorbance of oil at different wavelength. It was noted that in the buffer solution of pH 8, there was maximum absorbance of all the samples. This experimental study shows that both formulations have well sustained absorption through gastrointestinal tract. The results indicate that the rate of absorption exhibited to be higher in MCLT compared to ACLT. This work helps to develop a new method to evaluate intestinal absorption and compare formulation for therapeutic efficacy and drug absorbance. ","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140771323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.47552/ijam.v15i1.3705
Gabhane Shilpa M, Waghmare Sandip, Khade Sachin S, Waghmare Gopal
Seborrheic keratosis is a typical, benign disorder that affects the ageing population. In Ayurveda it can be compared with Charmakeel which is one of the types of Kshudraroga. Acharya Sushruta described several therapy methods such as the internal administration of medication, external application, and para-surgical techniques in the treatment of Charmakeel. In present case report 26 years old female patient visited in OPD complaining of two thick, black patches on right ankle joint with mild itching since 6 months. The case was diagnosed as Charmakeel. Patient was treated with Shamana Chikitsa Mahamanjishthadi kashay, Aarogyavardhini Vati, Punarnavadi mandur, PanchtiktaGhrut guggul as internal medication and Panchvalkaladi taila for local application. Treatment was given for two months and Shodhana Chikitsa (Raktamokshana by Jalauka) was carried out at every 15 days interval during treatment. Patient reported with significant results. Follow-up was taken upto 6 months and patient had no any complaints or recurrence. This case shows that Ayurveda can treat keratosis effectively and without any side effect.
{"title":"A case study on Ayurvedic management of Seborrhoeic Keratosis with special reference to Charmakeel","authors":"Gabhane Shilpa M, Waghmare Sandip, Khade Sachin S, Waghmare Gopal","doi":"10.47552/ijam.v15i1.3705","DOIUrl":"https://doi.org/10.47552/ijam.v15i1.3705","url":null,"abstract":"Seborrheic keratosis is a typical, benign disorder that affects the ageing population. In Ayurveda it can be compared with Charmakeel which is one of the types of Kshudraroga. Acharya Sushruta described several therapy methods such as the internal administration of medication, external application, and para-surgical techniques in the treatment of Charmakeel. In present case report 26 years old female patient visited in OPD complaining of two thick, black patches on right ankle joint with mild itching since 6 months. The case was diagnosed as Charmakeel. Patient was treated with Shamana Chikitsa Mahamanjishthadi kashay, Aarogyavardhini Vati, Punarnavadi mandur, PanchtiktaGhrut guggul as internal medication and Panchvalkaladi taila for local application. Treatment was given for two months and Shodhana Chikitsa (Raktamokshana by Jalauka) was carried out at every 15 days interval during treatment. Patient reported with significant results. Follow-up was taken upto 6 months and patient had no any complaints or recurrence. This case shows that Ayurveda can treat keratosis effectively and without any side effect.","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140792682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pruthvisara taila is a formulation indicated in the chikitsa of vrana as topical medicine in classical text Chakradatta. It contains Shuddha chitrakamoola, Shuddha vatsanabha, Shuddha karaveera, Nirgundi moola, Nadibeeja, Kanji and Karanja taila as the base. Aims and Objectives: Evaluation of wound healing property of Pruthvisara taila through experimental study. Methodology: Wound healing property in albino rats by excision wound healing model and its histopathology study. Results: Results of the study on the parameters assessed like percentage of wound contraction and histopathology study; percentage of wound closure was observed in Control group was 94.92%, in standard it was 93.73% and in test drug the percentage of wound contraction was 95.73%.The test drug, Pruthvisara taila showed more angiogenesis and formation of new blood vessels than standard group and in control group there was absence of formation of new blood vessels and proliferation of fibroblast cells. The control group did not show any collagen formation and scab formation the test drug Pruthvisara taila showed moderate formation and deposition compared to standard drug. Compared to control and standard group, the test drug, Pruthvisara taila selected in this study showed remarkable wound healing property in excised wound.
{"title":"Experimental study of Pruthvisara taila in excised wound model in Wistar albino rats","authors":"Archana Pagad, Abhayakumar Mishra, Vinay R Kadibagil, Sudhakar Bhat, Prasanna Mathad","doi":"10.47552/ijam.v15i1.4450","DOIUrl":"https://doi.org/10.47552/ijam.v15i1.4450","url":null,"abstract":" Pruthvisara taila is a formulation indicated in the chikitsa of vrana as topical medicine in classical text Chakradatta. It contains Shuddha chitrakamoola, Shuddha vatsanabha, Shuddha karaveera, Nirgundi moola, Nadibeeja, Kanji and Karanja taila as the base. Aims and Objectives: Evaluation of wound healing property of Pruthvisara taila through experimental study. Methodology: Wound healing property in albino rats by excision wound healing model and its histopathology study. Results: Results of the study on the parameters assessed like percentage of wound contraction and histopathology study; percentage of wound closure was observed in Control group was 94.92%, in standard it was 93.73% and in test drug the percentage of wound contraction was 95.73%.The test drug, Pruthvisara taila showed more angiogenesis and formation of new blood vessels than standard group and in control group there was absence of formation of new blood vessels and proliferation of fibroblast cells. The control group did not show any collagen formation and scab formation the test drug Pruthvisara taila showed moderate formation and deposition compared to standard drug. Compared to control and standard group, the test drug, Pruthvisara taila selected in this study showed remarkable wound healing property in excised wound.","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140796137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-04-01DOI: 10.47552/ijam.v15i1.4310
Gomathi R, U. Tn, Rajesh Kumar S
Universe has millions and millions of plants, but not all the plants have medicinal values. One such gift from nature is Sesame and is crowned as the “Queen of oilseed crops” due to its various medical properties like anti-inflammatory, antioxidant, anti- microbial etc. This research paper aims to compare the antioxidant activity of sesame leaf extract and sesame root extract with the standard ascorbic acid using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) assay. Different concentrations of the extracts and ascorbic acid were prepared (10 µg/mL, 20 µg/mL, 30 µg/mL, 40 µg/mL, and 50 µg/mL), and the antioxidant activity was measured. The results indicate that both sesame leaf extract and sesame root extract exhibit antioxidant activity, with sesame leaf extract showing slightly higher activity than sesame root extract. The findings suggest that sesame leaf extract has potential as a natural antioxidant source and could be used as an alternative to synthetic antioxidants. Overall, this study provides valuable information on the potential health benefits of sesame extracts and highlights the importance of natural antioxidants in preventing free radical damage and associated diseases.
{"title":"Comparative antioxidant activity of Sesame root and leaf extract - An In-vitro study","authors":"Gomathi R, U. Tn, Rajesh Kumar S","doi":"10.47552/ijam.v15i1.4310","DOIUrl":"https://doi.org/10.47552/ijam.v15i1.4310","url":null,"abstract":"Universe has millions and millions of plants, but not all the plants have medicinal values. One such gift from nature is Sesame and is crowned as the “Queen of oilseed crops” due to its various medical properties like anti-inflammatory, antioxidant, anti- microbial etc. This research paper aims to compare the antioxidant activity of sesame leaf extract and sesame root extract with the standard ascorbic acid using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) assay. Different concentrations of the extracts and ascorbic acid were prepared (10 µg/mL, 20 µg/mL, 30 µg/mL, 40 µg/mL, and 50 µg/mL), and the antioxidant activity was measured. The results indicate that both sesame leaf extract and sesame root extract exhibit antioxidant activity, with sesame leaf extract showing slightly higher activity than sesame root extract. The findings suggest that sesame leaf extract has potential as a natural antioxidant source and could be used as an alternative to synthetic antioxidants. Overall, this study provides valuable information on the potential health benefits of sesame extracts and highlights the importance of natural antioxidants in preventing free radical damage and associated diseases.","PeriodicalId":13751,"journal":{"name":"International Journal of Ayurvedic Medicine","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140779537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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