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Recent Unique Research for Enhancing Micropropagation Protocol of Balanites aegyptiaca (L.) Via Mammalian Sex Hormones 埃及巴兰虫(Balanites aegyptica, L.)微繁技术研究进展通过哺乳动物性激素
Pub Date : 2017-05-30 DOI: 10.22192/IJARBS.2017.04.05.002
H. E.A, Abd El-Kadder E.M, Aref M.S, E. E.A
In the present study, trials were done to investigate the influence of supplementing Murashige and Skoog’s culture medium with mammalian sex hormones (progesterone, estrogen, testosterone, human chorionic gonadotropin (HCG) and anabolic steroids on enhancing regeneration of Balanites aegyptiaca L., in vitro. For this purpose, nodal segment explants which were excised from in vitro grown plantlets (5-6 weeks old) were cultured on MS medium supplemented with 0.5 mg/L BAP in addition to progesterone, estrogen, testosterone, HCG and anabolic steroids (1.0, 3.0 and 5.0 mg/L each). For rooting, the regenerated shoots were cultured on half strength, hormone-free MS medium (as a control) or supplemented with 1.0 mg/L anabolic steroids alone or combined with β-cyclodextrin (10 and 20mg/L) and/or IBA (1.0 and 2.0 mg/L). Results of the present study have revealed that impregnating the culture medium with mammalian sex hormones (MSHs) had, in the great majority, significant positive effects on number of shoots per explant, shoot length, number of leaves per single shoot, and leaf area of Balanites aegyptiaca after 5 weeks of incubation. For example, impregnating the culture medium with 5.0 mg/L testosterone had resulted in the maximum shoot length, number of leaves and leaf area (15.33cm, 29 and 2.78cmrespectively). The highest significant shoots number per explant reached up 4.0using 1.0 mg/L testosterone. On other hand, the least significant number of shoots/nodal segment, shoot length, number of leaves and mean leaf area were(1.0, 1.93cm, 3.67, and 0.1cmrespectively)in response to treatment with 5.0 mg/L HCG. The results obtained showed also that half strength MS medium supplemented with 1.0 mg/L anabolic steroid hormone with or without 1.0 mg/L IBA had the highest significant rooting percentage (75%) and number of rootlets per shoot (5.67). The highest significant root length (5.50cm) was obtained when regenerants were cultured on half strength MS medium supplemented with 1.0 mg/L anabolic steroids hormones combined with 20.0 mg/l βcyclodextrin. The obtained regenerants were easily acclimatized within 4 weeks and transferred to greenhouse with a survival percentage of 95%. The results obtained in this study may indicate the promising future role that mammalian sex hormones (MSHs) can play in enhancement of shoot and root morphogenesis in vitro
本实验研究了在Murashige和Skoog培养基中添加哺乳动物性激素(孕酮、雌激素、睾酮、人绒毛膜促性腺激素(HCG)和合成代谢类固醇)对埃及巴兰虫体外再生的影响。为此,从5-6周龄的离体苗上取下节段外植体,在MS培养基上培养,MS培养基中添加0.5 mg/L BAP和黄体酮、雌激素、睾酮、HCG和合成代谢类固醇(分别为1.0、3.0和5.0 mg/L)。为了生根,再生芽在半强度无激素的MS培养基上培养(作为对照),或单独添加1.0 mg/L合成代谢类固醇,或与β-环糊精(10和20mg/L)和/或IBA(1.0和2.0 mg/L)联合培养。本研究结果表明,用哺乳动物性激素(MSHs)浸渍培养基,在培养5周后,对埃及巴兰(Balanites aegyptiaca)的外植体芽数、芽长、单枝叶数和叶面积均有显著的正影响。以5.0 mg/L的睾酮为培养基,茎长、叶数和叶面积最大,分别为15.33cm、29 cm和2.78cm。当睾酮浓度为1.0 mg/L时,每外植体显著芽数最高可达4.0个。5.0 mg/L HCG处理的芽数/节段数、芽长、叶数和平均叶面积最不显著(分别为1.0、1.93、3.67和0.1cm)。结果还表明,在添加1.0 mg/L合成代谢类固醇激素和不添加1.0 mg/L IBA的半强度MS培养基中生根率最高(75%),每枝生根数最高(5.67根)。再生体在添加1.0 mg/L合成代谢类固醇激素和20.0 mg/L β环糊精的半强度MS培养基上培养时,根长最高,为5.50cm。获得的再生体在4周内容易驯化,并转移到温室,成活率达95%。本研究结果可能预示着哺乳动物性激素(MSHs)在体外促进茎和根形态发生方面具有广阔的应用前景
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引用次数: 1
Effect of microbial inoculants on Growth and Yield of bhendi [Abelmoschus esculentus(L.)Moench] in field trial. 微生物接种剂对肉苁茸生长及产量的影响Moench在田间试验中。
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.024
U. S. sundari, A. Gandhi
An experiment was conducted in agriculture field, Karaikal during the season of JuneSeptember to observe the efficacy of isolated bacterial endophytes from the roots of bhendi as microbial inoculants in seeds on growth, yield and some biochemical constitutions of bhendi cv. Arkaanamika [Abelmoschus esculentus (L.) Moench] and the results revealed that the height of plant, number of branches, number of leaves, number of flowers, number of fruits, fruit weight, fruit length, fruit diameter and yield/plot were recorded maximum in T7and proved that the application of microbial inoculants and their combinations with inorganic fertilizers significantly influenced the growth, yield, quality and biochemical contents of bhendi. The combined application rates of 75% Chemical fertilizer with Azospirillum braziliense and Pseudomonas fluorescens gave the best performance of bhendi compared to other treatments. From the results, microbial inoculants willreduce the farmer’s budget for crop fertilization and inclusion of organic fertilizer in the combination will surely ensure production of crop under a less polluted environment.
本试验于6 - 9月在Karaikal农业大田进行,观察了从根中分离出的内生细菌作为种子微生物接种剂对种子生长、产量及部分生化指标的影响。Arkaanamika[绿毛鼠](L.)Moench]和结果显示,株高、枝数、叶数、花数、果数、果重、果长、果径和产量/畦均在t7中最高,证明微生物接种剂的施用及其与无机肥料的配施对白莲的生长、产量、品质和生化含量有显著影响。与其他处理相比,75%的氮肥配施巴西偶氮螺旋菌和荧光假单胞菌的效果最好。从结果来看,微生物接种剂可以减少农民对作物施肥的预算,在组合中加入有机肥肯定会确保作物在污染较少的环境下生产。
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引用次数: 1
ffect of addition trehalose and steps of freezing on sperm properties of bull frozen in liquid nitrogen. 添加海藻糖和冷冻步骤对液氮冷冻公牛精子性能的影响。
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.025
Qusay Mohammed Al-Badrany, Kreem Iwaid Al-Badry, F. Ibrahim, W. Y. Lateef
Object of study to improve semen preserve ability through the use of trehalose sugar as extender additive expressed in frozen semen and education effect steps of freezing on sperm properties. The research trail was carried on the semen samples collected from 4 bulls through a period of 16 weeks, were diluted with Tris-based extender containing different Trehalose concentrations (viz. 50, 100, 150,200) mM and control, evaluated for semen characteristics at steps of freezing (after dilution, after cooling and Post-Thawing). Results clearly indicated that, 100mM Trehalose group had significantly (P<0.05) higher individual motility in comparison to the 50Mm, 150mM, 200Mm and control group. Moreover, the sperm dead and abnormality was significantly (P<0.05) lower, when compared to the control group. The freezing process negatively affects (P < 0.05) the sperm parameters (individual motility, dead and abnormality), but the current study revealed that this effects were changes from treatment to another’s, it means there is interaction between effect steps of freezing and addition trehalose to Tris diluents of bull spermatozoa, however, overall, this effect was lower when the 100mM trehalose was added to this diluents. Conclussion: The addition of 100 Mm trehalose to TFEG diluents had their benefits on freezing-thawing bull semen. A step of freezing process was detrimental to bull sperm properties, but this effect was less when adding 100Mm trehalose to the diluted.
目的研究海藻糖作为冷冻精液中表达的扩展剂添加剂,提高精液的保存能力,以及冷冻对精子性能的影响步骤。在16周的时间里,研究人员采集了4头公牛的精液样本,用含有不同海藻糖浓度(即50、100、150、200)mM的tris扩增剂和对照进行稀释,在冷冻(稀释后、冷却后和解冻后)的步骤中评估精液特征。结果清楚地表明,100mM海藻糖组个体活力显著(P<0.05)高于50Mm、150mM、200Mm和对照组。与对照组相比,精子死亡和异常显著(P<0.05)降低。冷冻过程对精子参数(个体活力、死亡和异常)有负性影响(P < 0.05),但本研究表明,这种影响是不同处理之间的变化,说明牛精子Tris稀释液中冷冻和添加海藻糖的效果步骤之间存在交互作用,但总体而言,在Tris稀释液中添加100mM海藻糖时,这种影响较低。结论:在TFEG稀释液中添加100 Mm海藻糖对牛精液的冻融效果显著。冷冻过程中的一个步骤对公牛精子的性能有不利影响,但在稀释液中加入100Mm海藻糖对公牛精子的影响较小。
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引用次数: 1
Effect of Hizikia fusiformis extracts on reactive oxygen species mediated oxidative damage 梭梭菌提取物对活性氧介导的氧化损伤的影响
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.017
Jaeyeol Baek, Sun‐Young Lim
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引用次数: 2
Importance of the Tomato as such as medical plant 西红柿作为药用植物的重要性
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.015
H. Kheyrodin, Sadaf Kheyrodin
The tomato is a major vegetable crop that has achieve tremendous popularity over the last century. It is grown in practically every country of the world – in outdoor fields, greenhouses and net houses. The tomato plant is very versatile and the crop can be divided into two categories; fresh market tomatoes, which we are concerned with and processing tomatoes, which are grown only outdoors for the canning industry and mechanically harvested. In both cases, world production and consumption has grown quite rapidly over the past 25 years. Tomatoes can make people healthier and decrease the risk of conditions such as cancer, osteoporosis and cardiovascular disease. People who ate tomatoes regularly have a reduced risk of contracting cancer diseases such as lung, prostate, stomach, cervical, breast, oral, colorectal, esophageal, pancreatic, and many other types of cancer. Some studies show that tomatoes and garlic should be taken together at the same time to have its cancer preventive effects. Whatever it is, we really do not know how or why tomatoes work against cancers. We believe that lycopene and the newly discovered bioflavonoids in tomatoes are responsible as cancer fighting agents. Not only raw tomatoes but also cooked or processed tomato products such as ketchup, sauce, and paste, are counted as good sources of cancer prevention. Tomato is also good for liver health. Tomatoes, aside from being tasty, are very healthy as they are a good source of vitamins A and C. Vitamin A is important for bone growth, cell division and differentiation, for helping in the regulation of immune system and maintaining surface linings of eyes, respiratory, urinary and intestinal tracts. Vitamin C is important in forming collagen, a protein that gives structures to bones, cartilage, muscle and blood vessels. It also helps maintain capillaries, bones and teeth and aids in the absorption of iron.
西红柿是一种主要的蔬菜作物,在上个世纪获得了极大的普及。它在世界上几乎每个国家都有种植——在室外、温室和网房。番茄植物用途广泛,可分为两类;我们关注的新鲜市场西红柿和加工西红柿,这些西红柿只在户外种植,用于罐装工业和机械收获。在这两种情况下,世界生产和消费在过去25年里增长相当迅速。西红柿可以使人更健康,降低患癌症、骨质疏松症和心血管疾病的风险。经常吃西红柿的人患肺癌、前列腺癌、胃癌、宫颈癌、乳腺癌、口腔癌、结肠直肠癌、食道癌、胰腺癌和许多其他类型癌症的风险降低。一些研究表明,西红柿和大蒜应该同时食用,以达到预防癌症的效果。不管是什么原因,我们真的不知道西红柿抗癌的原理和原因。我们相信西红柿中的番茄红素和新发现的生物类黄酮是抗癌剂。不仅生番茄,煮熟或加工过的番茄制品,如番茄酱、酱汁和番茄酱,都被认为是预防癌症的良好来源。番茄对肝脏健康也有好处。西红柿除了美味之外,也是非常健康的,因为它们是维生素a和c的良好来源。维生素a对骨骼生长、细胞分裂和分化很重要,有助于调节免疫系统,维护眼睛、呼吸道、泌尿系统和肠道的表层。维生素C在形成胶原蛋白中很重要,胶原蛋白是一种为骨骼、软骨、肌肉和血管提供结构的蛋白质。它还有助于维持毛细血管、骨骼和牙齿,并有助于铁的吸收。
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引用次数: 1
Review on Estrus Synchronization and Its Application in Cattle 牛发情同步及其应用研究进展
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.010
L. Yizengaw
Numbers of estrus synchronization programmes are available in cattle based on the use of various hormones like progesterone, prostaglandin F2α and their various combinations with other hormones like estrogen and Gonadotrophin Releasing hormone (GnRH). Selection of appropriate estrus synchronization protocol should be made on the basis of management capabilities and expectations of the farmer. Synchronization of oestrus can be accomplished with the injection of prostaglandin F2α alone, but it needs proper detection of the ovarian status of the cows as prostaglandin F2α is active in only functional corpus luteum in between 8 to 17 days of estrous cycle. Progesterone may reduce fertility up to 14 percent, but short time progesterone exposure (less than 14 days) is beneficial. Addition of GnRH in the Progesterone or Prostaglandin based synchronization programme is helpful for more synchrony in estrus as GnRH may be helpful to synchronize the oestrous cycle in delayed pubertal heifers and post partum cows (Post partum anoestrum) and further a single, timed artificial insemination is possible with this method. New methods of synchronizing estrus in which the GnRH-PG protocol is preceded by progesterone treatment offer effective synchronization of estrus with high fertility.
根据黄体酮、前列腺素F2α等各种激素的使用,以及它们与雌激素和促性腺激素释放激素(GnRH)等其他激素的各种组合,牛的发情同步方案的数量是可用的。应根据农民的管理能力和期望选择合适的发情同步方案。单独注射前列腺素F2α可以实现奶牛的同步发情,但由于前列腺素F2α仅在发情周期的8 ~ 17天功能黄体中有活性,因此需要对奶牛卵巢状态进行适当的检测。黄体酮可使生育力降低14%,但短时间黄体酮暴露(少于14天)是有益的。在以孕酮或前列腺素为基础的同步程序中添加GnRH有助于更多的发情同步,因为GnRH可能有助于同步延迟发情的母牛和产后奶牛的发情周期(产后无发情),并且可以使用这种方法进行单次定时人工授精。同步发情的新方法,其中GnRH-PG方案之前,孕酮治疗提供了有效的同步发情与高生育能力。
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引用次数: 3
Isolation and screening of pyocyanin producing Pseudomonas spp. from soil 土壤中产pyocyanin假单胞菌的分离与筛选
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.020
Mayur Gahlout, H. Prajapati, P. Chauhan, Nilam C. Patel, Dhruti Solanki
Pseudomonas spp. produces large quantities of water soluble blue green phenazine pigment pyocyanin. The blue green chloroform soluble phenazine pigment extracted from Pseudomonas spp has antimicrobial activity. In present study a total of nine bacterial isolated screened from the different environment sample. The isolates obtained were gram negative, non-spore forming and exhibited fluorescence under UV light. The isolate showing blue green pigment on king’s B agar medium at a temperature of 35oC after 48-72 hr. of incubation and considered as having capabilities to produce pyocyanin.
假单胞菌产生大量的水溶性蓝绿色菲那嗪色素pyocyanin。从假单胞菌中提取的蓝绿色氯仿可溶性吩那嗪色素具有抗菌活性。本研究共从不同环境样品中分离出9种细菌。分离得到的菌株革兰氏阴性,不形成孢子,在紫外光下表现出荧光。分离物在35℃的king’s B琼脂培养基上培养48-72小时后呈现蓝绿色色素。被认为有能力产生花青素的。
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引用次数: 9
Antifeedant activity of different solvent extracts of Gliricidia sepium against third instar larvae of Helicoverpa armigera (Hubner) (Lepidoptera : Noctuidae) 不同溶剂提取物对棉铃虫三龄幼虫的拒食活性(鳞翅目:夜蛾科)
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.026
S. Jose, K. Sujatha
Plant based pesticides are known as phytopesticides. Phytopesticide formulations offer a more eco-friendly approach to Insect Pest Management than chemical pesticides. The biological compounds present in these formulations have several biological activities against insect pests. In the present work, an attempt has been made to study the antifeedant activity of Gliricidia sepium leaf extracts using three solvents, i.e; petroleum ether, ethyl acetate and methanol against third instar larvae of Helicoverpa armigera. All extracts exhibited as significant antifeedant activity at LC50. Methanol extract deterred feeding potential of the insect larvae by 62.38% at 1000 ppm, followed by ethyl acetate (46.67%) and petroleum ether (40.37%). Hence this naturally occurring plant extracts could be useful for managing the Helicoverpa armigera population.
植物基农药被称为植物农药。植物农药配方提供了一种比化学农药更环保的害虫管理方法。这些制剂中的生物化合物具有多种抗虫活性。本研究尝试用三种溶剂,即;石油醚、乙酸乙酯和甲醇对棉铃虫3龄幼虫的杀伤作用。在LC50时,各提取物均表现出显著的拒食活性。在1000 ppm浓度下,甲醇提取物对幼虫取食潜力的抑制率为62.38%,其次是乙酸乙酯(46.67%)和石油醚(40.37%)。因此,这种天然存在的植物提取物可以用于管理棉铃虫的数量。
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引用次数: 7
Impact of Industrial effluents on Water Quality of Betwa River near Mandideep, Raisen 工业废水对波兰曼迪普附近贝特瓦河水质的影响
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.023
M. Kulshrestha, Birjesh Singh, S. Pani
{"title":"Impact of Industrial effluents on Water Quality of Betwa River near Mandideep, Raisen","authors":"M. Kulshrestha, Birjesh Singh, S. Pani","doi":"10.22192/IJARBS.2017.04.04.023","DOIUrl":"https://doi.org/10.22192/IJARBS.2017.04.04.023","url":null,"abstract":"","PeriodicalId":13830,"journal":{"name":"International Journal of Advanced Research in Biological Sciences","volume":"71 1","pages":"169-181"},"PeriodicalIF":0.0,"publicationDate":"2017-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75941411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Molecular identification and characterization of Fusarium spp. associated with wheat grains 小麦穗部镰刀菌的分子鉴定与特性研究
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.011
M. Mahmoud, S. M. Shehata
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引用次数: 4
期刊
International Journal of Advanced Research in Biological Sciences
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