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Investigation of phytochemical screening and antimicrobial activity of Curcuma longa 姜黄的植物化学筛选及抑菌活性研究
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.021
P. Chauhan, Kruti Keni, Raxita D Patel
Many plants have been screened for antimicrobial activity and drug properties. The aim of the present study encourages the use of spices as alternative or supplementary medicine to reduce the burden of high cost, side effects and progressively increasing drug resistance of pathogens. In present study methanol and chloroform extracts of Curcuma longa (fresh and dry) were prepared from the rhizome. The antimicrobial properties of both of the extract were studied by testing the antibacterial as well as antifungal activity. The antibacterial test was done by agar well diffusion method against bacterial species such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, Protease vulgaris, Pseudomonas aeruginosa, Bacillus cereus, Bacillus megatarium, Bacillus subtilis and fungal species such as Aspergillus niger. The zone of inhibition of the extract were determined and compared with the standard drug streptomycin to know the efficiency. The methanol extract of rhizome was found to be more effective when compared with other solvent like chloroform. The phytochemical analysis of both of the extract revealed the presence of alkaloids, flavonoids, terpenoids, tannins, saponin and steroids.
许多植物的抗菌活性和药性已被筛选。本研究的目的是鼓励使用香料作为替代或补充药物,以减轻高昂的费用、副作用和逐渐增加的病原体耐药性的负担。以姜黄根茎为原料,分别制备了鲜姜黄和干姜黄的甲醇和氯仿提取物。通过对两种提取物的抑菌活性和抗真菌活性进行研究。采用琼脂孔扩散法对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌、普通蛋白酶、铜绿假单胞菌、蜡样芽孢杆菌、巨型芽孢杆菌、枯草芽孢杆菌等细菌和黑曲霉等真菌进行抑菌试验。测定其抑菌范围,并与标准药物链霉素进行比较,了解其抑菌效果。与氯仿等溶剂相比,用甲醇提取根茎的效果更好。两种提取物的植物化学分析显示存在生物碱、类黄酮、萜类、单宁、皂苷和类固醇。
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引用次数: 12
Changes in nutrients content of Radish (Raphanus sativus) under copper toxicity 铜中毒对萝卜营养物质含量的影响
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.012
P. Vijayarengan, M. C. Uthayam
In this experiment, we tried to explore the changes that are occurring in radish due to the treatment with copper. A pot culture experiment was conducted and copper was applied to radish (Raphanus sativus) plant. Macronutrients such as, nitrogen, phosphorus, potassium, sodium, calcium, magnesium were analysed for the treated plants. The results showed that the low concentrations of copper level (50 mg kg) enhanced the macronutrients while higher concentration of copper(100 250 mg kg) in the soil decreased these parameters.
在这个实验中,我们试图探索由于铜处理在萝卜中发生的变化。采用盆栽试验方法,对萝卜植株施用铜。对处理过的植株进行了氮、磷、钾、钠、钙、镁等常量营养元素的分析。结果表明,低铜浓度(50 mg kg)可提高土壤中微量元素的含量,高铜浓度(100 ~ 250 mg kg)可降低土壤中微量元素的含量。
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引用次数: 2
Effects of fatty acids, nutrients and whole body vibration on bone histomorphometry, mechanical properties and metabolic parameters in male rat 脂肪酸、营养物质和全身振动对雄性大鼠骨组织形态学、力学性能和代谢参数的影响
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.018
H. Bahadoran, S. Salmasi, M. Karimfar, R. Shirazi, M. Asadi, M. Naghii, G. Torkaman, M. Mofid
The aims of this study are to investigate the effects of consumption of fatty acids, nutrients and application of vibration on bone histomorphometry and mechanical properties in addition to metabolic bone parameters in rat. Fifty six male Wistar rats were divided into seven independent groups and treated for 8 weeks as followings: (1) Control (received regular rat chaw food and drinking water); (2) RV (regular food + vibration); (3) SPM (regular food + vibration + calcium + vitamin D + boron); (4) CA (SPM + canola oil); (5) SU (SPM + sunflower oil); (6) CA + SU (SPM + Canola oil + Sunflower oil); and (7) CO (SPM + coconut oil). After 8 weeks, plasma samples were analyzed for vitamin D, parathyroid hormone, calcitonin, testosterone, free testosterone, and estrogen levels. The right femur bone was excised for histomorphometric and geometric analyses. The left femur and fifth lumbar vertebra were also used for evaluation of mechanical properties. Our results indicated that compact bone area and number of osteocytes in all treatment groups and energy to maximal load in RV compared to control group were significantly increased (P<0.05). The decline in trabecular separation area, femoral neck maximal load, and femoral neck stiffness in SPM compared to control, RV and both groups were significant (P<0.05). Also, several significant changes in spongy bone and trabecular separation areas, femoral neck maximal load and stiffness, lumbar maximal load and lumbar energy to maximal load between control and different treatment groups were detected (P<0.05). Decrease of trabecular separation area in CA, CA+SU, and CO groups compared to control group and femoral neck deformation in SU compared to CA group were significant. Finally, significant increase in estrogen level in CO group compared to all other groups was detected. Our findings demonstrated that combination of whole body vibration and coconut oil has beneficial effects on bone histomorphometry, mechanical properties and metabolic parameters.
本研究的目的是探讨脂肪酸、营养物质的消耗和振动对大鼠骨组织形态学和力学性能以及骨代谢参数的影响。将56只雄性Wistar大鼠分为7个独立组,按以下方法治疗8周:(1)对照组(给予常规大鼠咀嚼食物和饮水);(2) RV(正常食物+振动);(3) SPM(常规食物+振动+钙+维生素D +硼);(4) CA (SPM +菜籽油);(5) SU (SPM +葵花油);(6) CA + SU (SPM +菜籽油+葵花油);(7) CO (SPM +椰子油)。8周后,分析血浆样本的维生素D、甲状旁腺激素、降钙素、睾酮、游离睾酮和雌激素水平。切除右侧股骨进行组织形态学和几何分析。左股骨和第五腰椎也被用来评估机械性能。结果表明,与对照组相比,各治疗组大鼠骨致密面积、骨细胞数量和最大负荷能量均显著增加(P<0.05)。与对照组、RV组及两组相比,SPM组股骨小梁分离面积、股骨颈最大负荷、股骨颈刚度下降均有统计学意义(P<0.05)。对照组和不同治疗组的海绵骨和小梁分离面积、股骨颈最大负荷和刚度、腰椎最大负荷和腰椎能量对最大负荷的变化均有统计学意义(P<0.05)。CA组、CA+SU组和CO组股骨小梁分离面积较对照组减少,SU组股骨颈变形较CA组显著。最后,与其他各组相比,CO组雌激素水平显著升高。我们的研究结果表明,全身振动和椰子油的组合对骨组织形态学、力学性能和代谢参数有有益的影响。
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引用次数: 0
Mie Tyndal scattering of spheroidal RBC and WBC: T-matrix calculations 球形红细胞和白细胞的Mie Tyndal散射:t矩阵计算
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.013
F. Rzayeva, A. M. Efendiyev, Nargiz A. Isayeva, Amirullah M.Mamedov
The electromagnetic problem has been solved for randomly oriented nonspherical and inhomogeneous structures with complex refractive indices 1.33018–2:09 x 10i (immersed in water) due to the T -matrix method. The light scattering properties of spheroidal erythrocytes/leukocytes for each condition were obtained using so-called Mie theory. In this study, the spheroidal erythrocyte is assumed to have a radius of 2:78 mm; giving it a volume equivalent to the average actual erythrocyte, and is illuminated by a plane wave of l 1⁄4 873 nm: The T -matrix elements are obtained by numerical integration in each condition. Comparisons for erythrocytes/leukocytes with oxygenated hemoglobin and immersed in water, and its results for large, randomly oriented spheroids, show that the applicability range of the scattering process depends on the imaginary part of the refractive index and is changed for different elements of the scattering matrix.
用T矩阵法求解了复折射率为1.33018-2:09 x 10i(浸入水中)的随机取向非球面非均匀结构的电磁问题。球型红细胞/白细胞在各种情况下的光散射特性是用所谓的米氏理论得到的。在本研究中,假设球形红细胞的半径为2:78 mm;使其体积相当于实际红细胞的平均体积,并由1 / 4 873 nm的平面波照射。在每种情况下,T矩阵元素通过数值积分得到。对含氧血红蛋白的红细胞/白细胞和浸泡在水中的红细胞/白细胞的比较,以及对大的、随机定向的球体的结果表明,散射过程的适用范围取决于折射率的虚部,并且随着散射矩阵的不同元素而改变。
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引用次数: 1
Isolation and characterization of detergent degrading bacteria from soil 土壤中洗涤剂降解菌的分离与鉴定
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.022
H. Prajapati, P. Chauhan, Mayur Gahlout, Bijal Patel, Hemangi Patel
Detergents (surface active agents) are chemical compounds which are largely used for washing purpose. In present time the use of detergent has increased in household and industries, which causes numerous problems in sewage treatment facilities and causes toxic effect on organisms and eco system. Present study reports the isolation, morphological and estimation of detergent degrading bacteria. Soil samples collected from various detergent contaminated region ( Vapi and Valsad Region). Detergent was used as a sole source of carbon to enumerate detergent degrader. Total 16 isolates (S1..... S16) were obtained from soil sample, using basal salt medium. S7 isolate as shows maximum percentage of degradation at 1% concentration of detergent. The analysis shows that isolated organism belongs to Klebsiella species.
洗涤剂(表面活性剂)是主要用于洗涤目的的化合物。目前,洗涤剂在家庭和工业中的使用越来越多,这给污水处理设施带来了许多问题,并对生物和生态系统产生了毒性作用。本文报道了洗涤剂降解菌的分离、形态和鉴定。从各种洗涤剂污染地区(Vapi和Valsad地区)收集土壤样本。以洗涤剂为唯一碳源,列举了洗涤剂降解剂。共分离16株(S1.....S16)采用基础盐培养基从土壤样品中获得。S7分离物显示,在1%的洗涤剂浓度下,降解率最大。结果表明,分离的病原菌属克雷伯菌属。
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引用次数: 1
Isolation, screening and identification of cyanobacteria and its uses in bioremediation of industrial effluents and chromium sorption 蓝藻的分离、筛选和鉴定及其在工业废水生物修复和铬吸附中的应用
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.019
Mayur Gahlout, H. Prajapati, P. Chauhan, Leena Savande, P. Yadav
Blue Green Algae are Prokaryotic, Oxygenic photosynthetic, Autotrophic and some of them are able to fix atmospheric nitrogen. Cyanobacteria used in aquaculture, wastewater treatment, food, fertilizers. Its play an important role in maintainance and build-up of soil fertility, consequently increasing cereals growth and yield as a natural biofertilizer. In the present study, total 25 water sample were taken from of various ponds, lakes and rivers in valsad, vapi and surat region. A total of 8 cyanobacteria among 25 sample were isolated. Cyanobacteria grows well on algal culture broth and urea broth. The incubation period taken was 15-20 days for the growth of the cyanobacteria by using light energy. In microscopic examination cyanobacterial strain like oscillatoriasps, Nostocsps, Anabaena sps, ,Gloeocapsasps, PlectonemaspsandGloeothecespsetc were identified. The isolated cyanobacteria were used for further study of bioremediation of waste water and chromium sorption.
蓝藻是原核生物,具有氧光合作用和自养性,其中一些能够固定大气中的氮。蓝藻用于水产养殖、废水处理、食品、肥料。作为一种天然生物肥料,它在保持和积累土壤肥力方面发挥着重要作用,从而提高谷物的生长和产量。在本研究中,从valsad, vapi和surat地区的各种池塘,湖泊和河流中采集了25个水样。25份样品中共分离到8株蓝藻。蓝藻在海藻培养液和尿素培养液上生长良好。利用光能培养蓝藻的潜伏期为15 ~ 20天。镜检发现蓝藻菌株如振荡菌、Nostocsps、Anabaena sps、Gloeocapsasps、plectonemasps和gloeothecespsetc。将分离得到的蓝藻用于废水的生物修复和铬的吸附研究。
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引用次数: 5
Happy seeder zero tillage equipment for sowing of wheat in standing rice stubbles “快乐播种机”免耕设备,用于在直立稻谷中播种小麦
Pub Date : 2017-05-13 DOI: 10.22192/IJARBS.2017.04.04.014
M. Iqbal, M. Hussain, Naeem Faisal, J. Iqbal, A. Rehman, Maqsood Ahmad, Javed Akhtar Padyar
{"title":"Happy seeder zero tillage equipment for sowing of wheat in standing rice stubbles","authors":"M. Iqbal, M. Hussain, Naeem Faisal, J. Iqbal, A. Rehman, Maqsood Ahmad, Javed Akhtar Padyar","doi":"10.22192/IJARBS.2017.04.04.014","DOIUrl":"https://doi.org/10.22192/IJARBS.2017.04.04.014","url":null,"abstract":"","PeriodicalId":13830,"journal":{"name":"International Journal of Advanced Research in Biological Sciences","volume":"7 1","pages":"101-105"},"PeriodicalIF":0.0,"publicationDate":"2017-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76117436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Study of Candida albicans & Aspergillus fumigatus Sensitization among Egyptian patients with allergic Bronchial Asthma 埃及变应性支气管哮喘患者白色念珠菌和烟曲霉致敏的研究
Pub Date : 2017-05-12 DOI: 10.22192/IJARBS.2017.04.04.003
F. Ali, O. M. A. Latif, Hoda E. L. Sayed, R. H. A. E. Fotoh
Asthma is a major cause of disability, health resource utilization and poor quality of life for those who are affected. The global prevalence rates of doctor diagnosed asthma, clinical/treated asthma and wheezing in adults were 4.3%, 4.5%, and 8.6% respectively among 70 countries. Sensitization to fungi is an important factor in patients with allergic respiratory tract diseases, playing a major role in the development, persistence, and severity of lower airway disease, particularly asthma. The aim of this study was to determine the sensitization to Candida albicans and Aspergillus fumigatus fungi among Egyptian patients with allergic Asthma. This design of this study was cross-sectional, with enrolling of consecutive 100 adult asthmatic patients attended the allergy outpatient clinic; Ain Shams University hospital in the years 2015-2016. We found that 28 and 12 patients had positive Skin Prick Test to Candida albicans and Aspergillus fumigatus respectively, from those 22 and 10 were positive at Broncho-provocation test with positive predictive value of skin prick test 78.6% for Candida albicans and 83.3% for Aspergillus fumigatus.
哮喘是造成患者残疾、卫生资源利用和生活质量低下的主要原因。在70个国家中,医生诊断的哮喘、临床/治疗的哮喘和成人喘息的全球患病率分别为4.3%、4.5%和8.6%。真菌致敏是过敏性呼吸道疾病患者的一个重要因素,在下气道疾病,特别是哮喘的发展、持续和严重程度中起着重要作用。本研究的目的是确定埃及变应性哮喘患者对白色念珠菌和烟曲霉真菌的致敏性。本研究的设计是横断面的,连续入组100名在过敏门诊就诊的成年哮喘患者;艾因沙姆斯大学医院2015-2016年的数据。皮肤点刺试验白色念珠菌和烟曲霉分别阳性28例和12例,其中支气管激发试验阳性22例和10例,皮肤点刺试验白色念珠菌和烟曲霉的阳性预测值分别为78.6%和83.3%。
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引用次数: 0
Mycoflora associated with grain discolouration of common rice (Oryza sativa L.) cultivars and their management 普通水稻(Oryza sativa L.)品种籽粒变色相关菌群及其管理
Pub Date : 2017-05-12 DOI: 10.22192/IJARBS.2017.04.04.001
A. Mustafa, M. Mohsan
Six fungal species Alternaria alternata, Dreschelera oryzae, Curvularia oryzae, Fusarium moniliforme, Cercospora oryzae and Helminthosporium oryzae has been found associated with discoloured panicles of different varieties in the field. Helminthosporium oryzae and Alternaria alternata were the predominant species on all the varities. Similarly among the twelve treatment including control, Nativo and Kumulus outclassed all the chemicals in reducing discoloured panicle by 49.59% and 51.34% respectively as compared to control 67.40%.and hence improveing the paddy yield. Kumulus was the best treatment in reducing grain infection.
在田间发现与不同品种穗部变色有关的真菌种类有互交霉属、米Dreschelera、米曲霉属、monilifor镰刀菌属、米Cercospora和Helminthosporium oryzae。所有品种的优势种均为米绒球孢和互交霉。同样,在包括对照在内的12个处理中,土土和Kumulus在减少变色穗方面分别优于所有化学物质,分别为49.59%和51.34%,而对照为67.40%。从而提高水稻产量。Kumulus是减少粮食侵染的最佳药剂。
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引用次数: 4
Assessment of disinfection by-products levels in Aga surface water plant and its distribution system, Dakhlia, Egypt 埃及达赫利亚Aga地表水厂及其分配系统消毒副产物水平评估
Pub Date : 2017-05-12 DOI: 10.22192/ijarbs.2017.04.04.006
I. Moustafa, A. Bream, A. Awaad, A. Nader
Chlorine is the most common disinfectant used in drinking water that can reacts with naturally occurring organic matter to form a number of chemical byproducts as trihalomethanes (THMs). The aims of the present study are to investigate the levels of THMs in Aga surface water plant (ASWP) and its distribution system (DS)during the period between spring 2015 and winter 2016 as well as its levels in tanks in August 2015. Drinking water samples were collected from ASWP, in addition to4 sites away from the plant at different distances. Other parameters as temperature and residual chlorine were measured. The maximum concentrations of THMs in ASWP were detected during summer, 2015 while the minimum concentrations were recorded during autumn 2015. While in DS, the maximum value recorded atsite 4. Generally, all values of THMs during the study lies within the limits of the Egyptian standards 458 decreed in 2007.
氯是饮用水中最常用的消毒剂,它可以与天然存在的有机物发生反应,形成许多化学副产品,如三卤甲烷(THMs)。本研究的目的是调查2015年春季至2016年冬季期间阿加地表水厂(ASWP)及其分配系统(DS)中THMs的水平,以及2015年8月储罐中的THMs水平。饮用水样本采集自ASWP,以及距离电厂不同距离的4个站点。测量了温度、余氯等参数。ASWP中THMs的最高浓度出现在2015年夏季,最低浓度出现在2015年秋季。而在DS中,最大值记录在4点。总的来说,研究期间的所有THMs值都在2007年颁布的埃及标准458的限制范围内。
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引用次数: 5
期刊
International Journal of Advanced Research in Biological Sciences
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