This study aimed to investigate the histomorphological and immunohistochemical changes in the ovaries of Small East African goats during the dry and rainy seasons. A total of 180 ovaries were subjected to analysis using hematoxylin and eosin staining and immunohistochemistry for desmin and smooth muscle actin as cytoskeletal components. The results demonstrated the presence of healthy and atretic primordial, primary, secondary, and antral follicles in the ovaries during the dry and rainy seasons. In the dry season, the proportions of healthy primordial, primary, secondary, and antral follicles were 236 (95%), 85 (97%), 25 (78%), and 6 (27%), respectively, whereas the atretic follicles accounted for 12 (5%), 3 (3%), 7 (22%), and 16 (73%). Conversely, during the rainy season, 157 (99%), 61 (98%), 48 (93%), and 31 (92%) were classified as healthy follicles, while 2 (1%), 1 (2%), 4 (7%), and 3 (8%) were categorized as atretic. There was a significant difference in healthy and atretic follicles between the dry and rainy seasons (P < 0.05). Immunohistochemical analysis revealed positive staining for both desmin and smooth muscle actin in the theca cells of secondary and antral follicles, cortical stroma, and tunica media of blood vessels in healthy and atretic follicles during dry and rainy seasons. However, staining was not observed in the granulosa cells and oocytes. Overall, this study indicates that most antral follicles undergo obliterated follicular atresia during the dry season.
{"title":"Histomorphological and immunohistochemical studies of the ovary during dry and rainy seasons in Small East African goats (Capra hircus)","authors":"J.A. Ngou, W. Kimaro, C. Luziga","doi":"10.31893/avr.2023013","DOIUrl":"https://doi.org/10.31893/avr.2023013","url":null,"abstract":"This study aimed to investigate the histomorphological and immunohistochemical changes in the ovaries of Small East African goats during the dry and rainy seasons. A total of 180 ovaries were subjected to analysis using hematoxylin and eosin staining and immunohistochemistry for desmin and smooth muscle actin as cytoskeletal components. The results demonstrated the presence of healthy and atretic primordial, primary, secondary, and antral follicles in the ovaries during the dry and rainy seasons. In the dry season, the proportions of healthy primordial, primary, secondary, and antral follicles were 236 (95%), 85 (97%), 25 (78%), and 6 (27%), respectively, whereas the atretic follicles accounted for 12 (5%), 3 (3%), 7 (22%), and 16 (73%). Conversely, during the rainy season, 157 (99%), 61 (98%), 48 (93%), and 31 (92%) were classified as healthy follicles, while 2 (1%), 1 (2%), 4 (7%), and 3 (8%) were categorized as atretic. There was a significant difference in healthy and atretic follicles between the dry and rainy seasons (P < 0.05). Immunohistochemical analysis revealed positive staining for both desmin and smooth muscle actin in the theca cells of secondary and antral follicles, cortical stroma, and tunica media of blood vessels in healthy and atretic follicles during dry and rainy seasons. However, staining was not observed in the granulosa cells and oocytes. Overall, this study indicates that most antral follicles undergo obliterated follicular atresia during the dry season.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"196 11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78344127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In Linnaeus’s two-toed sloths (Choloepus didactylus), there is no distinct sexual dimorphism. It is an obstacle for gender determination from the external genitalia, especially in newborns or young sloths. Hence, easy, rapid, and reliable genetics-based methods for gender identification of the sloths are needed to continue captive breeding more successfully. In this study, a PCR-based technique that allows gender determination of two-toed sloths by using a sex-determining region Y (SRY) gene marker was described. The hair samples from young (suspect gender) and adult sloths (known gender) were used in genetic analysis. Initially, genomic DNA was isolated from hair root samples using Roche high pure PCR template preparation kit. The SRY primers were specifically designed based on the NCBI and Ensembl databases, and they were verified with the BLAST program concerning the two-toed sloth genome. PCR amplification with the SRY-specific primers was carried out by a programmable thermal cycler device using FastStart High Fidelity PCR System, Roche dNTPack. The samples were then electrophoresed on 2% agarose gels and were visualized by a gel documentation and analysis system. A specific band in the electrophoresis pattern is diagnostic for a male individual with a partial SRY region. Hence, the analysis demonstrated that the samples belonged to a male two-toed sloth. Two-toed sloth species are commonly preferred animals in zoos. Gender determination is inevitable for these animals in captivity to be raised successfully and healthily. Molecular genetic techniques allow high efficiency in taxonomic evaluations and gender identification in species that do not display sexual dimorphism. The PCR assay described here may be helpful for a rapid genetic analysis that can be widely used in gender determination for two-toed sloths.
在林奈的二趾树懒(Choloepus didactylus)中，没有明显的两性二态性。这是外部生殖器决定性别的障碍，特别是在新生儿或年轻的树懒中。因此，为了更成功地继续圈养繁殖，需要一种简单、快速、可靠的基于遗传学的树懒性别鉴定方法。在这项研究中，描述了一种基于pcr的技术，通过使用性别决定区Y (SRY)基因标记来确定二趾树懒的性别。年轻树懒(疑似性别)和成年树懒(已知性别)的毛发样本被用于基因分析。首先，使用Roche高纯PCR模板制备试剂盒从发根样品中分离基因组DNA。SRY引物是基于NCBI和Ensembl数据库专门设计的，并通过BLAST程序对二趾树懒基因组进行验证。使用FastStart High Fidelity PCR System, Roche dNTPack的可编程热循环装置对sys特异性引物进行PCR扩增。然后将样品在2%琼脂糖凝胶上电泳，并通过凝胶记录和分析系统进行可视化。在电泳模式的特定带是诊断男性个体与部分SRY区域。因此，分析表明这些样本属于一只雄性二趾树懒。两趾树懒是动物园里最受欢迎的动物。为了使这些圈养动物成功健康地成长，性别决定是不可避免的。分子遗传学技术允许在分类评估和性别鉴定的物种不显示性别二态性的高效率。本文所描述的聚合酶链式反应(PCR)试验可能有助于快速遗传分析，可广泛用于二趾树懒的性别确定。
{"title":"Gender determination by PCR assay for the sex-determining region Y (SRY) gene amplification in Linnaeus’s two-toed sloth (Choloepus didactylus)","authors":"S. Ardıçlı, B. Bozkurt, Ezgi Vatansever","doi":"10.30782/jrvm.1283245","DOIUrl":"https://doi.org/10.30782/jrvm.1283245","url":null,"abstract":"In Linnaeus’s two-toed sloths (Choloepus didactylus), there is no distinct sexual dimorphism. It is an obstacle for gender determination from the external genitalia, especially in newborns or young sloths. Hence, easy, rapid, and reliable genetics-based methods for gender identification of the sloths are needed to continue captive breeding more successfully. In this study, a PCR-based technique that allows gender determination of two-toed sloths by using a sex-determining region Y (SRY) gene marker was described. The hair samples from young (suspect gender) and adult sloths (known gender) were used in genetic analysis. Initially, genomic DNA was isolated from hair root samples using Roche high pure PCR template preparation kit. The SRY primers were specifically designed based on the NCBI and Ensembl databases, and they were verified with the BLAST program concerning the two-toed sloth genome. PCR amplification with the SRY-specific primers was carried out by a programmable thermal cycler device using FastStart High Fidelity PCR System, Roche dNTPack. The samples were then electrophoresed on 2% agarose gels and were visualized by a gel documentation and analysis system. A specific band in the electrophoresis pattern is diagnostic for a male individual with a partial SRY region. Hence, the analysis demonstrated that the samples belonged to a male two-toed sloth. Two-toed sloth species are commonly preferred animals in zoos. Gender determination is inevitable for these animals in captivity to be raised successfully and healthily. Molecular genetic techniques allow high efficiency in taxonomic evaluations and gender identification in species that do not display sexual dimorphism. The PCR assay described here may be helpful for a rapid genetic analysis that can be widely used in gender determination for two-toed sloths.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76381594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Aktar, Hasan Özkan, Kemal Bagci, Ümit Can Uzun, Mustafa Akkaşoğlu, Selvi Ali̇şan, H. Sagirkaya, S. Alçay
Saha şartlarında sperma almak ve tohumlama yapmak her zaman aynı koşullarda ve temiz şartlarda yapılamamaktadır. Bu çalışmada saha şartlarında üç farklı dozda intraservikal yolla suni tohumlama uygulaması sonucunda gebelik oranlarının araştırılması amaçlandı. Araştırmada Doğu Friz ırkı 60 adet koyun 3 gruba ayrıldı (n=20) ve 3 adet koç kullanıldı. Koyunlarda kızgınlıkların senkronize edilmesi amacıyla 20 mg flugeston asestat emdirilmiş süngerler 3 gruba da 12 gün süreyle yerleştirildi. Süngerlerin çıkarılacağı gün ovulasyonları senkronize etmek için tüm koyunlara 500 IU PMSG enjeksiyonu yapıldı ve 24 saat içerisinde tüm koyunların östrus gösterdiği tespit edildi. Aynı çiftlikteki aynı ırk 3 koçtan alınan spermalarla östrustaki koyunlar intraservikal yolla tohumlandı. Tohumlama dozları 1.grup için 100x106 spermatozoa/ml, 2.grup için 200x106 spermatozoa/ml, 3.grup için 400x106 spermatozoa/ml olarak belirlendi. Tohumlamaların ardından toplamda 28 adet (%47) koyunda fertilizasyon olmadığı ve tekrar kızgınlık gösterdiği, diğer 32 adet (%53) koyunun gebe kaldığı tespit edildi. Gerçekleştirilen istatistiksel analiz sonucunda 3.Grup (400x106) 1. Gruba (100x106) göre anlamlı olarak daha başarılı bulunmuştur (P
{"title":"The Effect of Different Insemination Doses on Pregnancy Success in East Frisian Sheep","authors":"A. Aktar, Hasan Özkan, Kemal Bagci, Ümit Can Uzun, Mustafa Akkaşoğlu, Selvi Ali̇şan, H. Sagirkaya, S. Alçay","doi":"10.30782/jrvm.1250057","DOIUrl":"https://doi.org/10.30782/jrvm.1250057","url":null,"abstract":"Saha şartlarında sperma almak ve tohumlama yapmak her zaman aynı koşullarda ve temiz şartlarda yapılamamaktadır. Bu çalışmada saha şartlarında üç farklı dozda intraservikal yolla suni tohumlama uygulaması sonucunda gebelik oranlarının araştırılması amaçlandı. Araştırmada Doğu Friz ırkı 60 adet koyun 3 gruba ayrıldı (n=20) ve 3 adet koç kullanıldı. Koyunlarda kızgınlıkların senkronize edilmesi amacıyla 20 mg flugeston asestat emdirilmiş süngerler 3 gruba da 12 gün süreyle yerleştirildi. Süngerlerin çıkarılacağı gün ovulasyonları senkronize etmek için tüm koyunlara 500 IU PMSG enjeksiyonu yapıldı ve 24 saat içerisinde tüm koyunların östrus gösterdiği tespit edildi. Aynı çiftlikteki aynı ırk 3 koçtan alınan spermalarla östrustaki koyunlar intraservikal yolla tohumlandı. Tohumlama dozları 1.grup için 100x106 spermatozoa/ml, 2.grup için 200x106 spermatozoa/ml, 3.grup için 400x106 spermatozoa/ml olarak belirlendi. Tohumlamaların ardından toplamda 28 adet (%47) koyunda fertilizasyon olmadığı ve tekrar kızgınlık gösterdiği, diğer 32 adet (%53) koyunun gebe kaldığı tespit edildi. Gerçekleştirilen istatistiksel analiz sonucunda 3.Grup (400x106) 1. Gruba (100x106) göre anlamlı olarak daha başarılı bulunmuştur (P","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"66 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83254109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bu çalışma Balıkesir ilinde kuzulardaki Eimeriosis türlerinin tayini ve yaygınlığının belirlenmesi amacıyla yapılmıştır. Balıkesir merkez, Edremit, Bigadiç ve Bandırma’da bulunan dört farklı çiftlikten, her birinden 150’şer adet olmak üzere toplamda 600 adet dışkı örneği toplanmıştır. Çalışma konusu olan kuzular 30-60, 60-90, 90-120 gün yaş aralığındadır. Her yaş aralığı için 25 erkek, 25 dişi olacak şekilde toplamda 50 kuzu seçilmiştir. Altı yüz hayvanın 438 adetinde Eimeriosis tespit edilmiştir. Balıkesir merkezde bulunan 150 hayvanın 110 tanesi, Edremit’te bulunan 150 hayvanın 108 tanesi, Bigadiç’te bulunan 150 hayvanın 96 tanesi, Bandırma’da bulunan 150 hayvanın 124 tanesi Eimeria pozitif bulunmuştur. Yedi farklı Eimeria türü dört çiftlikte de gözlemlenmiştir. Eimeria pozitif olan hayvanlarda en az 3, en fazla 6 Eimeria türü ile çoklu enfeksiyonlar saptanmıştır. Patojenik türler olan Eimeria ahsata, Eimeria ovinoidalis ve Eimeria bakuensis, Balıkesir bölgesinde tespit edilmiştir. Pozitif bulunan 438 hayvanın 367 tanesinde Eimeria ahsata (% 83,78), 331 tanesinde Eimeria parva (%75,57), 310 tanesinde Eimeria ovinoidalis (% 70,77), 306 tanesinde Eimeria bakuensis (% 69,86), 278 tanesinde Eimeria faueri (% 63,47), 260 tanesinde Eimeria intricata (% 59,36), 250 tanesinde Eimeria pallida (% 57,07) oranlarında tespit edilmiştir. Elde edilen sonuçlara göre E. ahsata (% 83,78) en fazla, E. pallida (% 57,07) en az gözlemlenen türler olmuştur. Bu çalışma ile Balıkesir bölgesinde kuzularda ilk kez Eimeria yaygınlık oranı belirlenmiş ve Eimeria tür tanımları yapılmıştır.
{"title":"Eimeriosis Prevalence and Species Identifications in Lambs in Balıkesir City Center and Its Districts","authors":"Mehmet Özüiçli, A. I. Diker","doi":"10.30782/jrvm.1105726","DOIUrl":"https://doi.org/10.30782/jrvm.1105726","url":null,"abstract":"Bu çalışma Balıkesir ilinde kuzulardaki Eimeriosis türlerinin tayini ve yaygınlığının belirlenmesi amacıyla yapılmıştır. Balıkesir merkez, Edremit, Bigadiç ve Bandırma’da bulunan dört farklı çiftlikten, her birinden 150’şer adet olmak üzere toplamda 600 adet dışkı örneği toplanmıştır. Çalışma konusu olan kuzular 30-60, 60-90, 90-120 gün yaş aralığındadır. Her yaş aralığı için 25 erkek, 25 dişi olacak şekilde toplamda 50 kuzu seçilmiştir. Altı yüz hayvanın 438 adetinde Eimeriosis tespit edilmiştir. Balıkesir merkezde bulunan 150 hayvanın 110 tanesi, Edremit’te bulunan 150 hayvanın 108 tanesi, Bigadiç’te bulunan 150 hayvanın 96 tanesi, Bandırma’da bulunan 150 hayvanın 124 tanesi Eimeria pozitif bulunmuştur. Yedi farklı Eimeria türü dört çiftlikte de gözlemlenmiştir. Eimeria pozitif olan hayvanlarda en az 3, en fazla 6 Eimeria türü ile çoklu enfeksiyonlar saptanmıştır. Patojenik türler olan Eimeria ahsata, Eimeria ovinoidalis ve Eimeria bakuensis, Balıkesir bölgesinde tespit edilmiştir. Pozitif bulunan 438 hayvanın 367 tanesinde Eimeria ahsata (% 83,78), 331 tanesinde Eimeria parva (%75,57), 310 tanesinde Eimeria ovinoidalis (% 70,77), 306 tanesinde Eimeria bakuensis (% 69,86), 278 tanesinde Eimeria faueri (% 63,47), 260 tanesinde Eimeria intricata (% 59,36), 250 tanesinde Eimeria pallida (% 57,07) oranlarında tespit edilmiştir. Elde edilen sonuçlara göre E. ahsata (% 83,78) en fazla, E. pallida (% 57,07) en az gözlemlenen türler olmuştur. Bu çalışma ile Balıkesir bölgesinde kuzularda ilk kez Eimeria yaygınlık oranı belirlenmiş ve Eimeria tür tanımları yapılmıştır.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85749101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Demirkan, Ece Kavas, M. Bozkurt, M. Korkmaz, İ. Kiliç, T. Kavas
Bovine gallstone (BGS) has been suggested to be used in the treatment of many diseases including neoplastic maladies in traditional medicine. Thus, this study aimed at evaluating the possible protective effects of BGS in mice colon tumor model. In the experiment, 8 week-old 30 male BALB/c mice weighing 25-30 g were randomly allocated into control group (n=6), tumor group ((n=6; tumor induced by 1,2- dimethylhydrazine? [DMH]), BGS>DMH group (n=6; BGS given prior to DMH challenge), BGS//DMH group (n=6; simultaneous administration of BGS and DMH) and DMH>BGS group (n=6; tumor induced by DMH then BGS given). Colonic tumor was induced by weekly subcutaneous injection of DMH at a 20 mg/kg/mouse dose for consequetive 11 weeks. BGS preparations were given to mice cyclically. Atypical cryptic foci formation in the colon was examined by histopathological staining. The lowest rate of colon tumor was noted in BGS>DMH group where BGS was given prior to tumor challenge. In addition simultaneous administration of DMH and BGS to mice inhibited tumor formation and development more significantly than those in other groups (except for BGS>DMH group). It was concluded that BGS significantly prevented colonic tumor formation if given to subjects before or during tumor challenge however, therapeutic capacity of BGS was not promising in this study.
{"title":"Preventive effects of bovine gallstone on murine colon neoplasia: room for improvement?","authors":"I. Demirkan, Ece Kavas, M. Bozkurt, M. Korkmaz, İ. Kiliç, T. Kavas","doi":"10.30782/jrvm.985209","DOIUrl":"https://doi.org/10.30782/jrvm.985209","url":null,"abstract":"Bovine gallstone (BGS) has been suggested to be used in the treatment of many diseases including neoplastic maladies in traditional medicine. Thus, this study aimed at evaluating the possible protective effects of BGS in mice colon tumor model. In the experiment, 8 week-old 30 male BALB/c mice weighing 25-30 g were randomly allocated into control group (n=6), tumor group ((n=6; tumor induced by 1,2- dimethylhydrazine? [DMH]), BGS>DMH group (n=6; BGS given prior to DMH challenge), BGS//DMH group (n=6; simultaneous administration of BGS and DMH) and DMH>BGS group (n=6; tumor induced by DMH then BGS given). Colonic tumor was induced by weekly subcutaneous injection of DMH at a 20 mg/kg/mouse dose for consequetive 11 weeks. BGS preparations were given to mice cyclically. Atypical cryptic foci formation in the colon was examined by histopathological staining. The lowest rate of colon tumor was noted in BGS>DMH group where BGS was given prior to tumor challenge. In addition simultaneous administration of DMH and BGS to mice inhibited tumor formation and development more significantly than those in other groups (except for BGS>DMH group). It was concluded that BGS significantly prevented colonic tumor formation if given to subjects before or during tumor challenge however, therapeutic capacity of BGS was not promising in this study.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85399021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Musa, J. Mwacharo, Saber Y. Adam, Demin Cai, Abdelkareem A. Ahmed
{"title":"Climate change, nutrigenomics, immune function and diseases and production in dairy cattle: a comprehensive review","authors":"H. Musa, J. Mwacharo, Saber Y. Adam, Demin Cai, Abdelkareem A. Ahmed","doi":"10.31893/avr.2023010","DOIUrl":"https://doi.org/10.31893/avr.2023010","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81282797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khalied H. Elshareef, H. Musa, Saber Y. Adam, Jaafar S. Fedailb, Demin Cai, Abdelkareem A. Ahmed
{"title":"Genetic polymorphism of the BMP15 gene in West African sheep (Fulani) breed in Sudan","authors":"Khalied H. Elshareef, H. Musa, Saber Y. Adam, Jaafar S. Fedailb, Demin Cai, Abdelkareem A. Ahmed","doi":"10.31893/avr.2023009","DOIUrl":"https://doi.org/10.31893/avr.2023009","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"204 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75876079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of vitamin C, electrolyte, and jaggery on transportation stress in different seasons on biochemical parameters of goats","authors":"D. Gupta, G. Kashyap, M. Ashutosh, A. Yadav","doi":"10.31893/avr.2023008","DOIUrl":"https://doi.org/10.31893/avr.2023008","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74945158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Increasing environmental temperatures as a result of climate change adversely affect livestock welfare and health. Moreover, temperatures increase the distribution and survival of parasites and infectious agents. Livestock diseases that cause significant economic losses are a worldwide concern. Toll like receptor 4 (TLR4) is an ideal marker gene candidate, due to the key role it plays in initiating the immune response against pathogens. In this study, the toll-interleukin-1 receptor (TIR) domain polymorphisms were investigated in the Turkish Holstein cattle by DNA sequencing. The effects of polymorphisms on the protein structure and function were evaluated by computational tools (I-Mutant Suite, Project Hope and PyMOL). The rs8193069 C>T polymorphism was detected in the TIR domain. The SNP causes threonine to isoleucine substitution at position 674 of the TLR4 protein which is the second amino acid of the TIR domain. The I-Mutant Suite predicted that Thr674Ile substitution can result in decreased protein stability (DDG= -0.40 Kcal/mol). Project Hope results showed that mutant and wild-type amino acids have different properties and that this can disturb the TIR domain. The Thr674Ile polymorphism observed in the TIR domain of TLR4 protein in Turkish Holstein cattle might affect the function of the TLR4 by causing physicochemical changes.
{"title":"Characterization and computational investigation of polymorphisms in the TIR domain of the TLR4 protein in Turkish Holstein cattle.","authors":"Sertaç Atalay","doi":"10.30782/jrvm.1197130","DOIUrl":"https://doi.org/10.30782/jrvm.1197130","url":null,"abstract":"Increasing environmental temperatures as a result of climate change adversely affect livestock welfare and health. Moreover, temperatures increase the distribution and survival of parasites and infectious agents. Livestock diseases that cause significant economic losses are a worldwide concern. Toll like receptor 4 (TLR4) is an ideal marker gene candidate, due to the key role it plays in initiating the immune response against pathogens. In this study, the toll-interleukin-1 receptor (TIR) domain polymorphisms were investigated in the Turkish Holstein cattle by DNA sequencing. The effects of polymorphisms on the protein structure and function were evaluated by computational tools (I-Mutant Suite, Project Hope and PyMOL). The rs8193069 C>T polymorphism was detected in the TIR domain. The SNP causes threonine to isoleucine substitution at position 674 of the TLR4 protein which is the second amino acid of the TIR domain. The I-Mutant Suite predicted that Thr674Ile substitution can result in decreased protein stability (DDG= -0.40 Kcal/mol). Project Hope results showed that mutant and wild-type amino acids have different properties and that this can disturb the TIR domain. The Thr674Ile polymorphism observed in the TIR domain of TLR4 protein in Turkish Holstein cattle might affect the function of the TLR4 by causing physicochemical changes.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85897694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Ci̇han, S. Kasap, G. Akgül, Fatma Zehra Evci̇, S. Şentürk
Blood glucose level is one of the most commonly used parameters in assessing the energy metabolism in dairy cows. The presented study aimed to compare the glucose concentrations in blood samples taken from the coccygeal vein, jugular vein, and mammary vein from the same animals at the same time. Healthy Holstein-Friesian dairy cows (n=25) in their second and third lactation were selected for the study. The blood samples were taken from the coccygeal vein, jugular vein, and V. subcutanea abdominis (mammary vein) at the same time from each animal. To analyze blood glucose, Free Style Optium Blood Glucose Test Strip (Abbott, Istanbul, TURKIYE) was used. In comparison to the blood taken from both the jugular vein and coccygeal vein, glucose levels in blood samples taken from the mammary vein were significantly low (P
{"title":"Comparison of Glucose Levels in Blood Samples Taken from Coccygeal, Jugular and Mammary Vein of Cows in Lactation","authors":"H. Ci̇han, S. Kasap, G. Akgül, Fatma Zehra Evci̇, S. Şentürk","doi":"10.30782/jrvm.1195467","DOIUrl":"https://doi.org/10.30782/jrvm.1195467","url":null,"abstract":"Blood glucose level is one of the most commonly used parameters in assessing the energy metabolism in dairy cows. The presented study aimed to compare the glucose concentrations in blood samples taken from the coccygeal vein, jugular vein, and mammary vein from the same animals at the same time. Healthy Holstein-Friesian dairy cows (n=25) in their second and third lactation were selected for the study. The blood samples were taken from the coccygeal vein, jugular vein, and V. subcutanea abdominis (mammary vein) at the same time from each animal. To analyze blood glucose, Free Style Optium Blood Glucose Test Strip (Abbott, Istanbul, TURKIYE) was used. In comparison to the blood taken from both the jugular vein and coccygeal vein, glucose levels in blood samples taken from the mammary vein were significantly low (P","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75531880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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