Ndaleh Wozerou Nghonjuyi, Emmanuel Ojong, D. Ewane, Bertha Anyizi, Blessing Fongwi Fonda, Takang Eta Ndip Bessong, Samba Blake Ndakwar
{"title":"Graded levels of dried garlic as a feed additive: impacts on growth, haematology, and serum biochemistry parameters of broilers in Buea, Cameroon","authors":"Ndaleh Wozerou Nghonjuyi, Emmanuel Ojong, D. Ewane, Bertha Anyizi, Blessing Fongwi Fonda, Takang Eta Ndip Bessong, Samba Blake Ndakwar","doi":"10.31893/avr.2023006","DOIUrl":"https://doi.org/10.31893/avr.2023006","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88464006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Impact of seasons and management factors on seminal attributes and frozen semen doses production in Holstein Friesian crossbred dairy bulls","authors":"D. Mandal, Mahesh Kumar, S. Tyagi","doi":"10.31893/avr.2023004","DOIUrl":"https://doi.org/10.31893/avr.2023004","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"129 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72393649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Christman, K. Lohmann, M. Hirschfeld, J. Munoz-Pérez, J. Garcia, J. Hernandez, H. Westermeyer, G. Lewbart
{"title":"Intraocular pressure using rebound tonometry in the Galápagos marine iguana (Amblyrhynchus cristatus)","authors":"J. Christman, K. Lohmann, M. Hirschfeld, J. Munoz-Pérez, J. Garcia, J. Hernandez, H. Westermeyer, G. Lewbart","doi":"10.52547/injvr.3.1.1","DOIUrl":"https://doi.org/10.52547/injvr.3.1.1","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90576643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Common clinical signs of fish diseases- Quick reference tool for laboratory animal research veterinarians","authors":"D. Palić","doi":"10.52547/injvr.3.1.39","DOIUrl":"https://doi.org/10.52547/injvr.3.1.39","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90669112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of trace metals in some organs of Carassius carassius (Linnaeus, 1758) fish and histopathological change in the Babil drainage network, Iraq","authors":"Ghusoon Alneamah, S. Alshkarchy, S. Al-Haider","doi":"10.52547/injvr.3.1.13","DOIUrl":"https://doi.org/10.52547/injvr.3.1.13","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84750672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neonatal döneminde en önemli mortalite sebebi olan buzağı ishalleri birçok faktöre bağlı olarak gelişmekte ve büyük ekonomik kayıplara sebep olmaktadır. Türkiye’ de bugüne kadar yapılan epidemiyolojik çalışmalarda, viral etkenlerin yeni doğan buzağılarda önemli oranda neonatal ishal olgularına neden olduğu gösterilmiştir. Bu çalışmada şiddetli ishal ve neonatal ölümlerin görüldüğü buzağılarda çoklu viral enfeksiyonların rolü araştırıldı. Çalışma kapsamında toplanan dışkı örnekleri (n=16) üç farklı hücre hattında (MDBK, HRT-18 ve MA-104) virus izolasyonuna alınmıştır. Tüm örneklere 4 kör pasaj işlemi ve sonrasında IPMA testi uygulandı. Bu süreçte araştırılan enterik virusların (BRV, BCoV, BVDV, BToV) hücre kültüründe izolasyonu yapılamadı. Çalışma kapsamında dışkı örneklerinden PCR ve Ag ELISA testlerinin sonuçlarına göre, ishal semptomu gösteren 16 buzağıdan alınan dışkı örneklerinin 14 (%87,5) adedinde araştırılan enterik virusların en az bir tanesi tespit edildi. Araştırılan olgularda tek BRV enfeksiyonuna rastlanmazken; 2 (%12,5) hayvanda tek BCoV, 2 (%12,5) hayvanda tek BVDV, 2 (% 12,5) hayvanda tek BToV enfeksiyonu saptandı. Diğer yandan 1 (%6,25) hayvanda BRV ve BVDV, 1 (%6,25) hayvanda BCoV ve BToV, 4 (%25) hayvanda BCoV ve BVDV, 1 (%6,25) hayvanda BVDV ve BToV içeren ikili enfeksiyonlar saptandı. 1 (%6,25) hayvanda ise BVDV, BToV ve BCoV olmak üzere enterik virusların oluşturduğu üçlü enfeksiyon saptandı. SDS-PAGE testinde akrilamid jel üzerinde RNA segmentlerinin bant profillerine göre (4/2/3/2) tespit edilen rotavirusun grup A'da yer aldığı gösterildi. Ayrıca çalışmada da PCR testi ile pozitif saptanan rotavirusun, G10P[11] genotipine sahip olduğu, genotip spesifik primerler kullanılarak tespit edildi. Bu araştırmada neonatal buzağı ishallerine sebep olabilen önemli viral etkenler olan BRV, BCoV, BVDV ve BToV’ un aynı işletmede eş zamanlı olarak görülebileceği ve şiddetli hastalık bulguları ve kayıplara neden olabileceği gösterilmiştir.
{"title":"Neonatal buzağı ishal olgularında enterik virusların (BRV, BCoV, BVDV, BToV) çoklu enfeksiyonu","authors":"Özer Ateş, Kadir Yeşi̇lbağ","doi":"10.30782/jrvm.1166863","DOIUrl":"https://doi.org/10.30782/jrvm.1166863","url":null,"abstract":"Neonatal döneminde en önemli mortalite sebebi olan buzağı ishalleri birçok faktöre bağlı olarak gelişmekte ve büyük ekonomik kayıplara sebep olmaktadır. Türkiye’ de bugüne kadar yapılan epidemiyolojik çalışmalarda, viral etkenlerin yeni doğan buzağılarda önemli oranda neonatal ishal olgularına neden olduğu gösterilmiştir. Bu çalışmada şiddetli ishal ve neonatal ölümlerin görüldüğü buzağılarda çoklu viral enfeksiyonların rolü araştırıldı. Çalışma kapsamında toplanan dışkı örnekleri (n=16) üç farklı hücre hattında (MDBK, HRT-18 ve MA-104) virus izolasyonuna alınmıştır. Tüm örneklere 4 kör pasaj işlemi ve sonrasında IPMA testi uygulandı. Bu süreçte araştırılan enterik virusların (BRV, BCoV, BVDV, BToV) hücre kültüründe izolasyonu yapılamadı. Çalışma kapsamında dışkı örneklerinden PCR ve Ag ELISA testlerinin sonuçlarına göre, ishal semptomu gösteren 16 buzağıdan alınan dışkı örneklerinin 14 (%87,5) adedinde araştırılan enterik virusların en az bir tanesi tespit edildi. Araştırılan olgularda tek BRV enfeksiyonuna rastlanmazken; 2 (%12,5) hayvanda tek BCoV, 2 (%12,5) hayvanda tek BVDV, 2 (% 12,5) hayvanda tek BToV enfeksiyonu saptandı. Diğer yandan 1 (%6,25) hayvanda BRV ve BVDV, 1 (%6,25) hayvanda BCoV ve BToV, 4 (%25) hayvanda BCoV ve BVDV, 1 (%6,25) hayvanda BVDV ve BToV içeren ikili enfeksiyonlar saptandı. 1 (%6,25) hayvanda ise BVDV, BToV ve BCoV olmak üzere enterik virusların oluşturduğu üçlü enfeksiyon saptandı. SDS-PAGE testinde akrilamid jel üzerinde RNA segmentlerinin bant profillerine göre (4/2/3/2) tespit edilen rotavirusun grup A'da yer aldığı gösterildi. Ayrıca çalışmada da PCR testi ile pozitif saptanan rotavirusun, G10P[11] genotipine sahip olduğu, genotip spesifik primerler kullanılarak tespit edildi. Bu araştırmada neonatal buzağı ishallerine sebep olabilen önemli viral etkenler olan BRV, BCoV, BVDV ve BToV’ un aynı işletmede eş zamanlı olarak görülebileceği ve şiddetli hastalık bulguları ve kayıplara neden olabileceği gösterilmiştir.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83749826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ceren Anlas, T. Bakirel, U. Çalışkan, Ceylan Dönmez, Fulya ÜSTÜN ALKAN, Oya ÜSTÜNER KELEŞ
Plant-based compounds have been used for medicinal purposes since ancient times, as easily accessible and low-cost treatment options. Despite the widespread belief that plants are quite safe and devoid of side effects, scientific studies have revealed the toxicity potential of active components of plants on healthy cells. The present study was designed to investigate in vitro cytotoxicity and genotoxicity potential of Achillea wilhelmsii C. Koch and Cuscuta arvensis Beyr., which are frequently used in traditional medicine. In this context, cytotoxicity evaluation of the extracts was performed by MTT (3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. Our cytotoxicity results indicated that the extract from A. wilhelmsii did not affect the viability of fibroblasts at any of the concentrations, but rather significantly stimulated cell proliferation from a concentration of 25 µg/mL. On the other hand, the extract from C. arvensis significantly reduced the viability of fibroblasts at all concentrations tested. In the second part of this research, the DNA damaging potential of the extracts was investigated by in vitro comet assay at non-cytotoxic concentrations. A. wilhelmsii extract caused a significant increase in the percentage of DNA in the tail (%TDNA), which is considered an indicator of DNA damage, only at the highest concentration, while C. arvensis extract did not significantly affect %TDNA at concentrations tested. The results of the present study indicated that the methanolic extract from A. wilhelmsii may be considered safe up to a concentration of 100 μg/mL, however, the cytotoxicity potential of C. arvensis may be a factor limiting its safe use.
植物基化合物自古以来就被用于医疗目的，作为易于获得和低成本的治疗选择。尽管人们普遍认为植物是非常安全的，没有副作用，但科学研究表明，植物的活性成分对健康细胞有潜在的毒性。本研究旨在探讨阿喀琉叶(Achillea wilhelmsii C. Koch)和秋葵(Cuscuta arvensis Beyr)的体外细胞毒性和遗传毒性潜力。这是传统医学中经常使用的。在这种情况下，采用MTT(3-[4,5-二甲基噻唑-2-基]-2,5-二苯基溴化四唑)测定法对提取物进行细胞毒性评价。我们的细胞毒性结果表明，在任何浓度下，黄颡鱼提取物都不影响成纤维细胞的活力，但在浓度为25µg/mL时，反而显著刺激细胞增殖。另一方面，在所有浓度的测试中，红草提取物都显著降低了成纤维细胞的活力。在本研究的第二部分，通过体外彗星试验研究了非细胞毒性浓度下提取物的DNA损伤潜力。只有在最高浓度下，黄颡鱼提取物才会显著增加尾DNA百分比(%TDNA)，这被认为是DNA损伤的一个指标，而黄颡鱼提取物在不同浓度下对%TDNA没有显著影响。本研究结果表明，在100 μg/mL的浓度下，黄菖蒲醇提物是安全的，但黄菖蒲的细胞毒性可能是限制其安全使用的一个因素。
{"title":"In vitro cytotoxicity and genotoxicity screening of Cuscuta arvensis Beyr. and Achillea wilhelmsii C. Koch in preclinical analysis for further drug design","authors":"Ceren Anlas, T. Bakirel, U. Çalışkan, Ceylan Dönmez, Fulya ÜSTÜN ALKAN, Oya ÜSTÜNER KELEŞ","doi":"10.30782/jrvm.1161358","DOIUrl":"https://doi.org/10.30782/jrvm.1161358","url":null,"abstract":"Plant-based compounds have been used for medicinal purposes since ancient times, as easily accessible and low-cost treatment options. Despite the widespread belief that plants are quite safe and devoid of side effects, scientific studies have revealed the toxicity potential of active components of plants on healthy cells. The present study was designed to investigate in vitro cytotoxicity and genotoxicity potential of Achillea wilhelmsii C. Koch and Cuscuta arvensis Beyr., which are frequently used in traditional medicine. In this context, cytotoxicity evaluation of the extracts was performed by MTT (3- [4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. Our cytotoxicity results indicated that the extract from A. wilhelmsii did not affect the viability of fibroblasts at any of the concentrations, but rather significantly stimulated cell proliferation from a concentration of 25 µg/mL. On the other hand, the extract from C. arvensis significantly reduced the viability of fibroblasts at all concentrations tested. In the second part of this research, the DNA damaging potential of the extracts was investigated by in vitro comet assay at non-cytotoxic concentrations. A. wilhelmsii extract caused a significant increase in the percentage of DNA in the tail (%TDNA), which is considered an indicator of DNA damage, only at the highest concentration, while C. arvensis extract did not significantly affect %TDNA at concentrations tested. The results of the present study indicated that the methanolic extract from A. wilhelmsii may be considered safe up to a concentration of 100 μg/mL, however, the cytotoxicity potential of C. arvensis may be a factor limiting its safe use.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90251259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was carried out to determine the effects of vinasse on bone strength and some material properties in laying hens with specific amounts of vinasse added to their diets. The study was the first to determine the mechanical properties of laying hens fed a diet supplemented with vinasse. The study was conducted on tibiotarsi of two hundred 94-week-old laying hens which were selected randomly from a commercial farm. Morphometric measurements were made using a digital scale and caliper, and mechanical data were collected from tibiotarsi using a three-point bending test with a compression-tension machine. The laying hens fed with vinasse had higher tibiotarsal length and weight than those in Control group. The groups fed with vinasse were significantly stiffer and had a higher breaking force than Control group (P=0.002, P
{"title":"Promising effects of vinasse use on bone strength in laying hens.","authors":"Bayram Süzer, Gülsüm Eren, Kerem Atamay","doi":"10.30782/jrvm.1201727","DOIUrl":"https://doi.org/10.30782/jrvm.1201727","url":null,"abstract":"This study was carried out to determine the effects of vinasse on bone strength and some material properties in laying hens with specific amounts of vinasse added to their diets. The study was the first to determine the mechanical properties of laying hens fed a diet supplemented with vinasse. The study was conducted on tibiotarsi of two hundred 94-week-old laying hens which were selected randomly from a commercial farm. Morphometric measurements were made using a digital scale and caliper, and mechanical data were collected from tibiotarsi using a three-point bending test with a compression-tension machine. The laying hens fed with vinasse had higher tibiotarsal length and weight than those in Control group. The groups fed with vinasse were significantly stiffer and had a higher breaking force than Control group (P=0.002, P","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75851924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Meat is an important source of protein with high biological value. Due to this importance, the production and consumption of meat and meat products such as minced meat, salami, sausage, meatballs and roasted meat has been increasing rapidly in recent years. In order to earn more money, some companies sell animal meats that are not consumed by the society by mixing them into meat products without considering the health, habits, ethical and cultural values of people. In this study, it is aimed to examine whether there are undesirable tissues and muscle tissue belonging to different species in meat products by making histological and molecular evaluations in raw meatball products offered for sale. Evaluations were made by taking samples of raw meatball products offered for sale by 6 different well known supermarkets. In the results we obtained, it was observed that the integrity of the muscle tissue was not impaired in sample 1, but the presence of cartilage fragments in places. In sample 2, different sizes of tendon fragments and nerve fiber bundles were detected, with less muscle tissue. In sample number 3, it was observed that the integrity of the muscle tissue was not impaired and the spice pieces were excessive. In sample number 4, it was observed that the integrity of the muscle tissue was partially damaged. In sample 5, large tendon fragments and abundant connective tissue were detected. In sample 6, an image was detected, suggesting that it was caused by too much use of the additive. In the RT-PCR results, no tissue belonging to different species was found in any of the 6 samples we received. These results, in addition to being inappropriate in terms of public health and consumer rights, also appear as attempts to gain unfair financial gain.
{"title":"Histological and Molecular Evaluation of Raw Meatball Products","authors":"T. Ilhan, A. Özcan","doi":"10.30782/jrvm.1170017","DOIUrl":"https://doi.org/10.30782/jrvm.1170017","url":null,"abstract":"Meat is an important source of protein with high biological value. Due to this importance, the production and consumption of meat and meat products such as minced meat, salami, sausage, meatballs and roasted meat has been increasing rapidly in recent years. In order to earn more money, some companies sell animal meats that are not consumed by the society by mixing them into meat products without considering the health, habits, ethical and cultural values of people. In this study, it is aimed to examine whether there are undesirable tissues and muscle tissue belonging to different species in meat products by making histological and molecular evaluations in raw meatball products offered for sale. Evaluations were made by taking samples of raw meatball products offered for sale by 6 different well known supermarkets. In the results we obtained, it was observed that the integrity of the muscle tissue was not impaired in sample 1, but the presence of cartilage fragments in places. In sample 2, different sizes of tendon fragments and nerve fiber bundles were detected, with less muscle tissue. In sample number 3, it was observed that the integrity of the muscle tissue was not impaired and the spice pieces were excessive. In sample number 4, it was observed that the integrity of the muscle tissue was partially damaged. In sample 5, large tendon fragments and abundant connective tissue were detected. In sample 6, an image was detected, suggesting that it was caused by too much use of the additive. In the RT-PCR results, no tissue belonging to different species was found in any of the 6 samples we received. These results, in addition to being inappropriate in terms of public health and consumer rights, also appear as attempts to gain unfair financial gain.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87289281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. O. Girişgin, Rafiga Gazi̇, B. Ahmedov, A. E. Ütük, L. Aydın
Nosemosis is one of the most dangerous infectious diseases of honeybees in Azerbaijan and the World. Nosema ceranae is the dominant species detected in Azerbaijan, and this study aimed to detect the prevalence of the infection in the country. For this purpose, an average of 100 honeybee samples were collected from 64 hives, 24 from three regions in the north and 40 from five areas in the south. In the lab, the abdomens of 50 bees from each group were dissected and crushed in a container, adding 50 ml of distilled water. According to obtained data after microscopic examination, the N. ceranae spores were found to have a high-level prevalence in northern regions (45.8% average) than in the southern areas (22.5% average) of Azerbaijan. Molecular diagnoses of Nosema-positive samples have already been made with PCR and N. ceranae has been detected from all regions. Data show us that the Nosemosis is common in Azerbaijan and is a significant thread in the beekeeping industry.
{"title":"PREVALENCE OF NOSEMA CERANAE IN NORTH AND SOUTH REGIONS OF AZERBAIJAN","authors":"A. O. Girişgin, Rafiga Gazi̇, B. Ahmedov, A. E. Ütük, L. Aydın","doi":"10.30782/jrvm.1121263","DOIUrl":"https://doi.org/10.30782/jrvm.1121263","url":null,"abstract":"Nosemosis is one of the most dangerous infectious diseases of honeybees in Azerbaijan and the World. Nosema ceranae is the dominant species detected in Azerbaijan, and this study aimed to detect the prevalence of the infection in the country. For this purpose, an average of 100 honeybee samples were collected from 64 hives, 24 from three regions in the north and 40 from five areas in the south. In the lab, the abdomens of 50 bees from each group were dissected and crushed in a container, adding 50 ml of distilled water. According to obtained data after microscopic examination, the N. ceranae spores were found to have a high-level prevalence in northern regions (45.8% average) than in the southern areas (22.5% average) of Azerbaijan. Molecular diagnoses of Nosema-positive samples have already been made with PCR and N. ceranae has been detected from all regions. Data show us that the Nosemosis is common in Azerbaijan and is a significant thread in the beekeeping industry.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83701131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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