Arachidonic acid (AA), which is released from phospholipids in the cell membrane by a variety of stimuli, has physiological and pathophysiological roles on the cardiovascular system. The current study designed to determine the effect of intravenously (iv) injected AA on the electrocardiography (ECG) of the anesthetized rats. The ECG waves obtained from the lead II were written by placing electrodes to limbs of the ketamine and xylazine mixture (50 mg/kg/20 mg/kg; im) anesthetized adult Spraque Dawley rats. AA (3 mg/kg; iv) statistically significantly (p
{"title":"Effect of Intravenously Injected Arachidonic Acid on Electrocardiography in Rats","authors":"Esra Kasikci, M. Yalçın","doi":"10.30782/jrvm.1124608","DOIUrl":"https://doi.org/10.30782/jrvm.1124608","url":null,"abstract":"Arachidonic acid (AA), which is released from phospholipids in the cell membrane by a variety of stimuli, has physiological and pathophysiological roles on the cardiovascular system. The current study designed to determine the effect of intravenously (iv) injected AA on the electrocardiography (ECG) of the anesthetized rats. \u0000The ECG waves obtained from the lead II were written by placing electrodes to limbs of the ketamine and xylazine mixture (50 mg/kg/20 mg/kg; im) anesthetized adult Spraque Dawley rats. AA (3 mg/kg; iv) statistically significantly (p","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85259245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heat shock proteins (Hsps) are molecular chaperones that play critical functions in the survival and development of cells. Hsps influence adaptive and innate immune responses and may promote cross-talk between the two systems. Superoxide dismutases (SODs) are metalloenzymes that play an essential role in the body's defense against oxidative stress by efficiently removing excess reactive oxygen species. This study is an experimental study that was conducted to determine the relationship between SOD1 and Hsp70 expression in the ileum during the post-hatching development of the broiler. In the study, samples were taken from ileum tissue of 0-, 21- and 42-day-old broilers were used as material. While the Hsp70 immunoreactivity observed in the epithelial cells was specific to a few cells on day 0, it was detected in more villus epithelial cells on days 21 and 42. The Hsp70 expression in the ileum increased from the age 0 to up to day 42, especially in villus epithelial cells. In sections stained by SOD1, the ileum's villus epithelial cells and smooth muscle cells showed an intracytoplasmic reaction. From day 21 to day 42, a regular increase in SOD1 expression was detected in the crypt and villus epithelial cells. As a remarkable finding, a more intense intracytoplasmic staining was detected in villus epithelial cells located at the apex of intestinal villi. In conclusion, it was observed that SOD1 and Hsp70 expression increased in the ileum tissue throughout post-hatching development in broilers with a positive correlation with age. Based on the histological findings, it can be concluded that SOD1 and Hsp70 play a critical protective role in the small intestine after hatching and contribute to the rapid development of the intestine.
{"title":"The relationship between SOD1 and Hsp70 expression in broiler ileum throughout post-hatching development","authors":"T. Ertugrul, Ş. Tütüncü, A. Ceylan, A. Çalık","doi":"10.30782/jrvm.1137803","DOIUrl":"https://doi.org/10.30782/jrvm.1137803","url":null,"abstract":"Heat shock proteins (Hsps) are molecular chaperones that play critical functions in the survival and development of cells. Hsps influence adaptive and innate immune responses and may promote cross-talk between the two systems. Superoxide dismutases (SODs) are metalloenzymes that play an essential role in the body's defense against oxidative stress by efficiently removing excess reactive oxygen species. This study is an experimental study that was conducted to determine the relationship between SOD1 and Hsp70 expression in the ileum during the post-hatching development of the broiler. In the study, samples were taken from ileum tissue of 0-, 21- and 42-day-old broilers were used as material. While the Hsp70 immunoreactivity observed in the epithelial cells was specific to a few cells on day 0, it was detected in more villus epithelial cells on days 21 and 42. The Hsp70 expression in the ileum increased from the age 0 to up to day 42, especially in villus epithelial cells. In sections stained by SOD1, the ileum's villus epithelial cells and smooth muscle cells showed an intracytoplasmic reaction. From day 21 to day 42, a regular increase in SOD1 expression was detected in the crypt and villus epithelial cells. As a remarkable finding, a more intense intracytoplasmic staining was detected in villus epithelial cells located at the apex of intestinal villi. In conclusion, it was observed that SOD1 and Hsp70 expression increased in the ileum tissue throughout post-hatching development in broilers with a positive correlation with age. Based on the histological findings, it can be concluded that SOD1 and Hsp70 play a critical protective role in the small intestine after hatching and contribute to the rapid development of the intestine.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91213397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The embryonic stem cells (ESCs) are pluripotent, self-renewing cells that able to differentiate into any of the germ layers involved in embryogenesis. However, the molecular mechanisms that control ESC pluripotency and differentiation remain poorly understood. The family of Pyruvate dehydrogenase kinase (PDK1-4), inactivates the mitochondrial pyruvate dehydrogenase complex via phosphorylation, plays a crucial role in the control of glucose homeostasis. In the current study, gene expression levels of PDK isoenzymes were analyzed on undifferentiated mouse embryonic stem cells (mESCs) and compared to mESCs induced to differentiate by removal of leukemia inhibitory factor (LIF) for 1, 3 and 5 days. Besides, we performed gene expression analysis of several genes related to pluripotency and differentiation. In addition, we also determined glucose uptake rates by a colorimetric assay kit in early differentiated and undifferentiated mESCs. Differently expression level of PDK isoenzymes in pluripotent and LIF-depleted mESCs suggest that they may have roles in differentiation and pluripotency of ESCs. Furthermore, this study lays the foundation for detailed investigation of molecular mechanisms underlying the roles of PDKs in the pluripotency and transition to differentiated state of ESCs.
{"title":"Changes in the Expression of Pyruvate Dehydrogenase Kinase Isoenzymes During Early Differentiation of Mouse Embryonic Stem Cells","authors":"Saime Güzel","doi":"10.30782/jrvm.1131148","DOIUrl":"https://doi.org/10.30782/jrvm.1131148","url":null,"abstract":"The embryonic stem cells (ESCs) are pluripotent, self-renewing cells that able to differentiate into any of the germ layers involved in embryogenesis. However, the molecular mechanisms that control ESC pluripotency and differentiation remain poorly understood. The family of Pyruvate dehydrogenase kinase (PDK1-4), inactivates the mitochondrial pyruvate dehydrogenase complex via phosphorylation, plays a crucial role in the control of glucose homeostasis. In the current study, gene expression levels of PDK isoenzymes were analyzed on undifferentiated mouse embryonic stem cells (mESCs) and compared to mESCs induced to differentiate by removal of leukemia inhibitory factor (LIF) for 1, 3 and 5 days. Besides, we performed gene expression analysis of several genes related to pluripotency and differentiation. In addition, we also determined glucose uptake rates by a colorimetric assay kit in early differentiated and undifferentiated mESCs. Differently expression level of PDK isoenzymes in pluripotent and LIF-depleted mESCs suggest that they may have roles in differentiation and pluripotency of ESCs. Furthermore, this study lays the foundation for detailed investigation of molecular mechanisms underlying the roles of PDKs in the pluripotency and transition to differentiated state of ESCs.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"122 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82643886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cryoprotectants have critical roles to prevent cell damages during cryopreservation. However, the adjustment of cryoprotectant concentration is also very crucial to protect cells from cryoprotectant toxicity. The present study was designed to investigate the effect of extenders including high concentration dimethyl sulfoxide (DMSO) on post-thaw rabbit sperm quality. Pooled rabbit sperm samples (n=7) were diluted and cryopreserved in extenders including 250 mmol/L Tris, 88 mmol/L citric acid, 47 mmol/L glucose, 1% sucrose and different concentrations of DMSO. The presence of high concentration DMSO (12 and 14%) in extender decreased sperm total and progressive motility (P < 0.01). The 8 and 10 percent DMSO supplementations in extender increased live sperm rates (P < 0.01). Live and intact acrosome or intact membrane sperm rates were detected higher in 8 and 10 DMSO groups (P < 0.05). When total intact membrane sperm rates were similar in all groups, total intact acrosome sperm rate was higher in 8 DMSO group compared to 12 DMSO group (P < 0.05). In conclusion, when 12 and 14 percent DMSO additions in extender adversely affected post-thaw sperm parameters, the presence of 8 percent DMSO in extender provided the highest post-thaw sperm quality.
{"title":"Effect of Extenders Including High Concentrations Dimethyl Sulfoxide (DMSO) on Post-Thaw Rabbit Sperm Parameters","authors":"N. Küçük","doi":"10.30782/jrvm.1125759","DOIUrl":"https://doi.org/10.30782/jrvm.1125759","url":null,"abstract":"Cryoprotectants have critical roles to prevent cell damages during cryopreservation. However, the adjustment of cryoprotectant concentration is also very crucial to protect cells from cryoprotectant toxicity. The present study was designed to investigate the effect of extenders including high concentration dimethyl sulfoxide (DMSO) on post-thaw rabbit sperm quality. Pooled rabbit sperm samples (n=7) were diluted and cryopreserved in extenders including 250 mmol/L Tris, 88 mmol/L citric acid, 47 mmol/L glucose, 1% sucrose and different concentrations of DMSO. The presence of high concentration DMSO (12 and 14%) in extender decreased sperm total and progressive motility (P < 0.01). The 8 and 10 percent DMSO supplementations in extender increased live sperm rates (P < 0.01). Live and intact acrosome or intact membrane sperm rates were detected higher in 8 and 10 DMSO groups (P < 0.05). When total intact membrane sperm rates were similar in all groups, total intact acrosome sperm rate was higher in 8 DMSO group compared to 12 DMSO group (P < 0.05). In conclusion, when 12 and 14 percent DMSO additions in extender adversely affected post-thaw sperm parameters, the presence of 8 percent DMSO in extender provided the highest post-thaw sperm quality.","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76731799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survey of Arsenic concentration in the goat's blood, wool and liver at Kurdistan Province, Iran","authors":"S. Fakour","doi":"10.52547/injvr.2.1.39","DOIUrl":"https://doi.org/10.52547/injvr.2.1.39","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"417 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77845306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluating the antioxidant activity of epidermal mucus extract in marine fishes Anguilla anguilla and Brachirus orientalis","authors":"G. Hemapoojavalli, S. Bragadeeswaran","doi":"10.52547/injvr.2.1.27","DOIUrl":"https://doi.org/10.52547/injvr.2.1.27","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"26 11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80730135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Masterson, T. R. Locklear, D. Páez-Rosas, J. Munoz-Pérez, C. Ortega, G. Lewbart, S.L. Vaden
{"title":"Intraocular pressure using rebound tonometry in the San Cristobal Galapagos tortoise (Chelonoidis chathamensis)","authors":"C. Masterson, T. R. Locklear, D. Páez-Rosas, J. Munoz-Pérez, C. Ortega, G. Lewbart, S.L. Vaden","doi":"10.52547/injvr.2.1.13","DOIUrl":"https://doi.org/10.52547/injvr.2.1.13","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75345430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The nutritional effect of probiotic Lactobacillus acidophilus on intestinal microbial flora and inhibition of Shiga toxin (Stx2) gene expression in zebrafish (Danio rerio) exposed to Escherichia coli O157:H7","authors":"P. Salimi, R. Kazempoor","doi":"10.52547/injvr.2.1.1","DOIUrl":"https://doi.org/10.52547/injvr.2.1.1","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81244652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Nahavandi, I. Adeshina, H. Rameshi, S. Nooraei, Y. Abdollahzadeh
{"title":"Different disease control methods in shrimp aquaculture: A review","authors":"R. Nahavandi, I. Adeshina, H. Rameshi, S. Nooraei, Y. Abdollahzadeh","doi":"10.52547/injvr.2.1.19","DOIUrl":"https://doi.org/10.52547/injvr.2.1.19","url":null,"abstract":"","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"152 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81723213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Güner, I. Kisadere, H. Tavşanlı, Serpil KAHYA DEMİRBİLEK, A. Keskin
The aim of the study was to investigate the effect of different intravaginal treatment strategies on the vaginal discharge score, vaginal microbiota, bacterial and Enterobacteriaceae counts in nulliparous Merino ewes. All ewes (n=45) received intravaginal sponges containing 60 mg medroxyprogesterone acetate for 7 days and allocated into three equal groups (n=15). Sponges were injected Lactobacillus plantarum cell-free supernatant (SUPER), enrofloxacin (ENRO), or physiologic saline (CON) prior to sponge insertion. At sponge removal, 500 IU equine chorionic gonadotropin (eCG) were administered in all ewes. For the detection of vaginal microbiota, bacterial and Enterobactericeae counts, samples were collected prior to sponge insertion, at sponge withdrawal, and 48 h later after sponge withdrawal. Vaginal discharge score was not different in ENRO (2.26±0.18) and SUPER (2.20±0.14) compared to CON (2.46±0.16). The time-dependent alteration was significant for the mean bacterial and Enterobacteriaceae count in all groups (P
{"title":"Comparison of the Efficacy of Enrofloxacin and Lactobacillus Plantarum Cell-Free Supernatant Treatments on Vaginitis in Ewes","authors":"B. Güner, I. Kisadere, H. Tavşanlı, Serpil KAHYA DEMİRBİLEK, A. Keskin","doi":"10.30782/jrvm.1139354","DOIUrl":"https://doi.org/10.30782/jrvm.1139354","url":null,"abstract":"The aim of the study was to investigate the effect of different intravaginal treatment strategies on the vaginal discharge score, vaginal microbiota, bacterial and Enterobacteriaceae counts in nulliparous Merino ewes. All ewes (n=45) received intravaginal sponges containing 60 mg medroxyprogesterone acetate for 7 days and allocated into three equal groups (n=15). Sponges were injected Lactobacillus plantarum cell-free supernatant (SUPER), enrofloxacin (ENRO), or physiologic saline (CON) prior to sponge insertion. At sponge removal, 500 IU equine chorionic gonadotropin (eCG) were administered in all ewes. For the detection of vaginal microbiota, bacterial and Enterobactericeae counts, samples were collected prior to sponge insertion, at sponge withdrawal, and 48 h later after sponge withdrawal. Vaginal discharge score was not different in ENRO (2.26±0.18) and SUPER (2.20±0.14) compared to CON (2.46±0.16). The time-dependent alteration was significant for the mean bacterial and Enterobacteriaceae count in all groups (P","PeriodicalId":13839,"journal":{"name":"International Journal of Applied Research in Veterinary Medicine","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90671783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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