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COMPARATIVE EFFECT OF THREE DIFFERENT ENERGY SOURCES ON HEMATOLOGY AND SERUM BIOCHEMISTRY OF RABBITS 三种不同能量源对家兔血液学和血清生化的影响比较
Pub Date : 2021-02-19 DOI: 10.47604/ijb.1223
Sani Babangida Salihu
Purpose: This study examined the effect of three different energy sources on hematology and serum biochemistry of rabbits. Methodology: Eighteen seven-weeks old rabbits where randomly divided into three groups (A, B and C), each consisting of six rabbits. Group A, B and C were given maize, sorghum and millet respectively as their sources of energy and were fed for a period of five weeks before sample collection and analysis. Findings: The results showed, that there was no significant different (P>0.05) in the hematology and serum biochemistry. Nevertheless, the red blood cells in all the treatment fell below the normal range. It was observed that the alkaline phosphatase of all the experimental rabbits were above the normal range. This study suggests that our test materials were not toxic to the muscle, kidney and liver of rabbits at 52.21% inclusion level. Unique contribution to theory, practice and policy: This implies that sorghum or millet can completely replace maize in rabbit diet with no adverse effect on the hematology and serum biochemistry parameters of rabbits.
目的:研究三种不同能量源对家兔血液学和血清生化的影响。方法:18只7周龄的家兔随机分为A、B、C三组,每组6只。A组、B组和C组分别饲喂玉米、高粱和谷子作为能量源,饲养5周后进行样品采集和分析。结果:两组患者血液学、血清生化指标无显著差异(P>0.05)。然而,在所有的治疗中,红细胞都低于正常范围。实验家兔碱性磷酸酶均高于正常范围。本研究表明,我们的实验材料在52.21%的包合水平下对家兔的肌肉、肾脏和肝脏没有毒性。理论、实践和政策上的独特贡献:说明高粱或谷子完全可以替代家兔饲粮中的玉米,且对家兔血液学和血清生化指标无不良影响。
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引用次数: 0
NUTRIENT COMPOSITION, ANTIOXIDANT COMPONENTS AND ASCORBIC ACID CONTENT RESPONSE OF PEPPER FRUIT (Capsicum annuum L.) CULTIVARS GROWN UNDER SALT STRESS 辣椒果实营养成分、抗氧化成分及抗坏血酸含量的响应盐胁迫下生长的品种
Pub Date : 2021-02-19 DOI: 10.47604/ijb.1224
Hand M.J., Nono G.V., Tonfack L.B, Youmbi E., Taffouo V.D.
Purpose: Salinity stress occurs due to the accumulation of high levels of salts in soil, which ultimately leads to the impairment of plant growth and crop loss. Stress tolerance-inducing compounds have a remarkable ability to improve growth and minimize the effects of salinity stress without negatively affecting the environment by controlling the physiological and molecular activities in plants. Methodology: The pots were arranged in a complete randomized design with one plant per pot and four replicates per treatment and carried out in 2017 and 2018 to study the influence of four levels of NaCl (0, 50, 100 and 200 mM) on the antioxidant, ascorbic acid, organic and inorganic compounds of three pepper fruits cultivars (‘Granada’, ‘Goliath’ and ‘Nobili’) at mature stage. Findings: The results obtained showed that salinity decreased the mineral content (Ca, K, Zn, Mg, Mn, Iron, S and P), relative water content, and agromorphological parameters of pepper fruit. This decrease was accompanied by a significant increase of Na, soluble proteins, proline content, soluble sugar (fructose, glucose) and antioxidants, including total phenolics and flavonoids, and reduced ascorbic acid and β-carotene content. It could be an indicator of salinity tolerance which explains the maintenance of a good water status among the studied plants. However, a varietal difference response to salt stress was observed between the studied varieties. Indeed, the varieties Granada and Nobili are characterized by their vigour in absence as in the presence of salt. Under the studied salinity level there was an enhancement of health-promoting compounds (phenolic compounds, flavonoids, β-carotene and ascorbic acid) synthesis in pepper fruits, with signifcant changes in other quality parameters. Unique contribution to theory, practice and policy: It is concluded that varieties ‘Granada’ and ‘Nobili’ is more tolerant to salinity compared to the variety ‘Goliath’. Hence, they have a significant role to play in agriculture, food, and economy.
目的:盐渍化胁迫是由于土壤中高水平盐的积累而产生的,最终导致植物生长受损和作物损失。耐胁迫诱导化合物通过控制植物的生理和分子活性,在不影响环境的情况下,显著提高植物的生长和减少盐胁迫的影响。方法:采用完全随机设计,每罐1株,每个处理4个重复,于2017年和2018年进行盆栽试验,研究4种水平NaCl(0、50、100和200 mM)处理对3个辣椒品种(格拉纳达、歌利亚和诺比利)成熟期抗氧化剂、抗坏血酸、有机和无机化合物的影响。结果表明:盐度降低了辣椒果实的钙、钾、锌、镁、锰、铁、硫、磷等矿质元素含量、相对含水量和农态参数。这种下降伴随着钠、可溶性蛋白、脯氨酸含量、可溶性糖(果糖、葡萄糖)和抗氧化剂(包括总酚类和类黄酮)的显著增加,以及抗坏血酸和β-胡萝卜素含量的降低。这可能是耐盐性的一个指标,解释了所研究植物保持良好的水分状态。但不同品种对盐胁迫的响应存在差异。事实上,品种格拉纳达和诺比利的特点是他们的活力在没有盐的存在。在不同盐度条件下,辣椒果实中酚类、黄酮类、β-胡萝卜素和抗坏血酸等有益健康物质的合成有所增加,其他品质参数变化显著。对理论、实践和政策的独特贡献:得出的结论是,品种“格拉纳达”和“诺比利”比品种“歌利亚”更耐盐。因此,它们在农业、食品和经济中发挥着重要作用。
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引用次数: 0
INFLUENCE OF INTRAPERITONEAL ADMINISTRATION OF CERIUM OXIDE ON BLOOD PARAMETERS AND LIPID PROFILE IN MICE (MUS MUSCULUS) 腹腔注射氧化铈对小鼠血液参数和血脂的影响
Pub Date : 2021-02-19 DOI: 10.47604/ijb.1221
Samaher Sabri Hammid, Sami Jeber Al-Maliky
Purpose: White laboratory mice Mus musculus L. strain BALB / C were used to study the effects cerium oxide on the hematological parameters and lipid profile values. Results: Results showed a significant decrease (p≤0.05) in the RBCs' hemoglobin Hb 'and PCV' parameters as well as the presence of a significant in the mean erythrocyte MCV volume in the two doses (2.5'and .3.75) micro liters / kg ' , while there was no significant difference in hemoglobin (MCH) and the mean concentration of hemoglobin in red blood cells (MCHC) when compared with the control group, while the two doses did not differ significantly in PCV, Hb, RBCs, while the two doses differed significantly between them in MCV. There is significant difference in the level of triglycerides (TG, LDL) and HDL (compared with the control group) for both doses, and it was found that there was a significant difference between the low and high dose (2.'3.75) micro liters / kg in the HDL level. TG, LDL) compared with the control group at the probability level (P≤0.05), while there was no difference between the high and low dose in the level of cholesterol and (VLDL).
目的:研究氧化铈对小家鼠BALB / C株血液学指标和血脂指标的影响。结果:结果显示,与对照组相比,2个剂量组(2.5'和3.75 ')患者红细胞血红蛋白Hb和PCV参数显著降低(p≤0.05),平均红细胞MCV体积显著降低(p≤0.05),而血红蛋白(MCH)和红细胞血红蛋白平均浓度(MCHC)无显著差异,PCV、Hb、红细胞、而两种剂量在MCV中存在显著差异。两种剂量的甘油三酯(TG, LDL)和HDL水平(与对照组相比)均有显著差异,并且发现低剂量和高剂量(2.3.75)微升/ kg的HDL水平存在显著差异。TG、LDL)与对照组相比,在概率水平上差异有统计学意义(P≤0.05),而高、低剂量组的胆固醇、VLDL水平差异无统计学意义(P < 0.05)。
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引用次数: 0
EXTRACTION AND QUANTIFICATION OF INTRACELLULAR LIPID AS POTENTIAL BIOFUEL FROM ALGAL BIOMASS 从藻类生物量中提取和定量作为潜在生物燃料的细胞内脂质
Pub Date : 2021-02-19 DOI: 10.47604/ijb.1222
Auyo, M. I, A. S., A. A.
Purpose: Fossil fuels occuring as ancient algae deposit is the major source of energy that we energise our engines today. It is a limited non-renewable resource that will eventually run out. Algae have been reported to generate renewable fuels known as biofuel. It is in this regards that this work was carried out to determine, extract and quantify intracellular lipid in algal biomass for subsequent use as biofuel. Methodology: Four average sized ponds within Federal University Dutse Campus, Nigeria (11.00̊ N to 13.00̊ N and longitude 8.00̊ E to 10.15̊ E and altitude 465.5m). were randomly selected and 20ml pond water were collected from top, middle and bottom of each pond. 60ml pond water was taken to laboratory and were processed by first preparing the media.  3ml NPK gel fertilizer was mixed with distilled water and autoclave at 121°C for 15 min. Bold's Basal Medium (BBM) consisting stock solution and BBM consisting of trace elements were separately mixed with distilled water. 10 ml of the stock solution and 1.0 ml of the trace elements were autoclave at 121°C for 15 min. The media (BBM and fertilizer) were cooled at room temperature, antibiotics (penicillin G, di-hydro-streptomycin sulfate and gentamycin sulfate) were added and pH adjusted to 7.5 and the media were kept ready for used. Isolations of species were carried out by serial dilution using BBM and under microscope sterile syringe and needles were used to isolate some target cells from the samples. Thus, four different algal species; Euglena, Spirogyra, Selenastrum and Chlorella were isolated and identified.  Culturing of the microalgae isolates was carried out by transferring into 50 ml growth chamber containing BBM and NPK fertilizer media separately and cultured using sunlight for 10 days under controlled pH.  Two successfully grown species; Spirogyra and Selenastrum were transferred aseptically into four constructed photobioreactors containing 400 ml liquid media; two containing BBM and the other fertilizer media. Nile Red was used to stain the algal wet biomass and observed under microscope and subsequently photographed. Microalgal cultures were dewatered by repeated centrifugations at 4000 rpm and the supernatants were discarded and the micoralgal biomass rinsed with deionised water to remove the residual salt and later dried using thermostatic drying oven and subsequently grinded. Growths were determined through dry weight determination and growth percentage was obtained as:  dried biomass of a species from each medium/total dried biomass of the species x 100. The dried algal biomass was extracted and evaporated and extracts were heated using vacuum rotary evaporator to separate the solvent (chlorofoam) from the extracts. Transesterification was carried out and first prepared solution was poured into a conical flask containing 5.2 g lipid of Selenastrum species and the second solution was poured into a conical flask containing 2 g lipid of Spirogyra spp. Both flasks were heated
目的:作为古老藻类沉积物的化石燃料是我们今天为发动机提供能量的主要来源。它是一种有限的不可再生资源,最终会耗尽。据报道,藻类可以产生被称为生物燃料的可再生燃料。正是在这方面进行了这项工作,以确定,提取和量化藻类生物质中的细胞内脂质,以便后续用作生物燃料。方法:尼日利亚联邦大学Dutse校区内四个平均大小的池塘(11.00°N ~ 13.00°N,经度8.00°E ~ 10.15°E,海拔465.5m)。随机抽取,每个池塘的上、中、底各取20ml池水。取60ml池水至实验室,先配制培养基进行处理。将3ml NPK凝胶肥与蒸馏水混合,在121℃下蒸15 min。将含有原液的Bold’s basic Medium (BBM)和含有微量元素的BBM分别与蒸馏水混合。取原液10 ml,微量元素1.0 ml, 121℃高压灭菌15 min。室温冷却培养基(BBM和肥料),加入抗生素(青霉素G、硫酸二氢链霉素和硫酸庆大霉素),调节pH至7.5,待用。物种分离采用BBM连续稀释法,显微镜下无菌注射器和针头从样本中分离出部分靶细胞。因此,有四种不同的藻类;分离鉴定了绿藻属、水绵属、硒藻属和小球藻。将分离的微藻分别移入含有BBM和NPK肥料培养基的50 ml生长室中,在控制ph下,日光下培养10 d。将Spirogyra和Selenastrum无菌转移到四个构建的光生物反应器中,反应器中含有400 ml液体培养基;两种含有BBM和其他肥料介质。用尼罗红对藻类湿生物量进行染色,在显微镜下观察并拍照。微藻培养物在4000转/分的转速下反复离心脱水,丢弃上清,微藻生物质用去离子水冲洗以去除残盐,然后用恒温干燥箱干燥,随后研磨。通过测定干重来确定生长,生长百分比为:每种培养基中某物种的干生物量/该物种的总干生物量× 100。对干燥的藻类生物质进行提取和蒸发,并使用真空旋转蒸发器对提取液进行加热,以分离提取液中的溶剂(氯泡沫)。进行酯交换反应,将第一种溶液倒入含有硒属植物5.2 g脂质的锥形瓶中,将第二种溶液倒入含有Spirogyra属植物2 g脂质的锥形瓶中,在磁力搅拌器上恒温加热,冷凝器保持在60℃。m相分离与净化一起进行,其中去除肥皂和其他杂质,得到的溶液仍然是易燃的生物柴油。研究结果:虽然绿藻和小球藻在初始培养基中不能生长,但Spirogyra和Selenastrum都能产生大量的生物柴油,其中Selenastrum的产量高于Spirogyra,因此是一种潜在的燃料来源。
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引用次数: 0
Assessment of Major Depressive Disorders through Neuroimaging Studies and their Treatment Methods 通过神经影像学研究评估重度抑郁症及其治疗方法
Pub Date : 2021-02-09 DOI: 10.46300/91011.2021.15.4
Jevetha Vijayadasan, Diksha Raghunathan, Sivakumar Rajagopal, Rahul Soangra
Many mental disorders are caused due to improper regulation of the brain and depression is one such. It affects both children and adults and is very common among teenagers. There are many challenges clinicians face regarding the management of this disease. These challenges have prompted the development of various neuroimaging techniques that effectively diagnose the condition. The main techniques are Magnetic resonance imaging (MRI) and Positron emission tomography (PET) which have gained momentum over the years. Advanced MRI techniques help study certain regions of the brain such as hippocampus and amygdala. Effective treatments for depression include antidepressant medications and brain stimulation techniques. Although treatments are effective for a lot of people, there is still room for improvement. This article (1) presents background on depression, its types, symptoms and risk factors; (2) elaborates the neuroimaging techniques used and reviews the various techniques adopted over the years to study depression; and (3) discusses the treatment methods that can be practised to cure depression. KeywordsBrain Stimulation Techniques, Depression,
许多精神障碍都是由于大脑调节不当造成的,抑郁症就是其中之一。它影响儿童和成人,在青少年中很常见。关于这种疾病的管理,临床医生面临许多挑战。这些挑战促使各种有效诊断这种疾病的神经成像技术的发展。磁共振成像(MRI)和正电子发射断层扫描(PET)是近年来发展迅速的主要技术。先进的核磁共振技术有助于研究大脑的某些区域,如海马体和杏仁核。治疗抑郁症的有效方法包括抗抑郁药物和脑刺激技术。虽然治疗方法对很多人都有效,但仍有改进的余地。本文(1)介绍抑郁症的背景、类型、症状和危险因素;(2)阐述了近年来用于抑郁症研究的神经影像学技术,并对其进行了综述;(3)讨论了治疗抑郁症的方法。关键词:脑刺激技术;抑郁症;
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引用次数: 2
Stress Classification Using K-means Clustering and Heart Rate Variability from Electrocardiogram 基于k均值聚类和心电图心率变异性的压力分类
Pub Date : 2021-01-18 DOI: 10.46300/91011.2020.14.32
Mingu Kang, Siho Shin, Jaehyo Jung, Y. Kim
In this study, we propose a method to classify individuals under stress and those without stress using k-means clustering. After extracting the R and S peak values from the ECG signal, the heart rate variability is extracted using a fast Fourier transform. Then, a criterion for classifying the ECG signal for the stress state is set, and the stress state is classified through k-means clustering. In addition, the stress level is indicated using the R − Speak value. This method is expected to be applied to the U-healthcare field to help manage the mental health of people suffering from stress. Keywords— K-means Clustering, Electrocardiogram (ECG), Heart Rate Variability (HRV), Fast Fourier Transform (FFT)
在本研究中,我们提出了一种基于k-均值聚类的压力个体和无压力个体的分类方法。在提取心电信号的R和S峰值后,利用快速傅里叶变换提取心率变异性。然后,设定心电信号的应激状态分类准则,通过k-均值聚类对应激状态进行分类;另外,用R−Speak值表示应力水平。该方法有望应用于u保健领域,以帮助管理压力患者的心理健康。关键词:k均值聚类,心电图,心率变异性,快速傅里叶变换(FFT)
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引用次数: 2
Acute and Sub-chronic Toxicity of Condensate Produced From Olive Mill Wastewater Using Solar Energy in Mice 橄榄厂太阳能废水冷凝液对小鼠的急性和亚慢性毒性研究
Pub Date : 2021-01-11 DOI: 10.46300/91011.2021.15.2
B. Al-Trad, S. Gharaibeh, Ameerah Qeshawy, M. A. Zoubi, Almuthanna K. Alkaraki, S. Omari, Malek Shehab
Olive mill wastewater (OMW) is one of the environmental challenges associated with the olive oil industry. This study was carried out to investigate the potential acute and sub-chronic toxicity of oral treatment of OMW condensate in mice. Different doses (250, 500, 1000, 2000, 4000, 8000 mg/kg) were applied once to investigate acute toxicity. Sub-chronic toxicity was investigated using thirty mice; two groups with (500, 4000 mg/kg/body weight) doses along with one control group. Acute toxicity study results showed that the LD50 was greater than the highest tested dose with no signs of systemic toxicity, mortality, or behavioral changes. In addition, the sub-chronic investigation did not show significant changes in behavior, body weight, and vital organs weight/body weight ratio along with no observed differences in the studied hematological parameters. Condensate dose of 500 mg/kg did not show significant differences in the levels of blood urea nitrogen (BUN), alanine aminotransferase and aspartate aminotransferase (AST). However, the AST serum level was significantly decreased and the serum level of BUN was increased at the dose of 4000 mg/kg. Results suggest that single and repeated oral doses of olive condensate administered orally are safe in mice.
橄榄油厂废水(OMW)是与橄榄油工业相关的环境挑战之一。本研究旨在探讨口服水杨酸对小鼠的急性和亚慢性毒性。不同剂量(250、500、1000、2000、4000、8000 mg/kg)一次性施用,观察急性毒性。30只小鼠进行亚慢性毒性研究;两组剂量分别为(500、4000毫克/公斤/体重),另设一个对照组。急性毒性研究结果显示,LD50大于最高测试剂量,没有出现全身毒性、死亡或行为改变的迹象。此外,亚慢性调查未显示行为、体重和重要器官重量/体重比发生显著变化,所研究的血液学参数也未观察到差异。凝结水剂量500 mg/kg对血尿素氮(BUN)、谷丙转氨酶(AST)和谷草转氨酶(丙氨酸转氨酶)水平无显著影响。4000mg /kg剂量组血清AST水平显著降低,BUN水平显著升高。结果表明,单次和多次口服橄榄凝聚物对小鼠是安全的。
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引用次数: 0
Arrhythmia Detection Algorithm using GoogLeNet and Generative Adversarial Network with Lifelog Signals 基于GoogLeNet和生成对抗网络的心律失常检测算法
Pub Date : 2021-01-11 DOI: 10.46300/91011.2021.15.1
Siho Shin, Jaehyo Jung, Mingu Kang, Y. Kim
Arrhythmia is a cardiovascular disease with an irregular heartbeat, which can lead to a heart attack if it lasts for an excessive amount of time. Because the symptoms of arrhythmia occur irregularly, the heart needs to be monitored for a lengthy time period. This study suggests an arrhythmia diagnosis algorithm using GoogLeNet and a GAN. Because the algorithm proposed in this study can add to the number of data using a GAN, it can accurately diagnose an arrhythmic occurrence from measured lifelog over a short period of time. The classification of ECG data using GoogLeNet and a GAN showed an accuracy of approximately 99%.
心律失常是一种伴有不规则心跳的心血管疾病,如果持续时间过长,可能会导致心脏病发作。由于心律失常的症状发生不规律,需要长时间监测心脏。本研究提出了一种基于GoogLeNet和GAN的心律失常诊断算法。由于本研究中提出的算法可以增加使用GAN的数据数量,因此它可以在短时间内从测量的生命日志中准确地诊断出心律失常的发生。使用GoogLeNet和GAN对心电数据进行分类,准确率约为99%。
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引用次数: 0
Ants’ Acquisition of A Symbol for Zero and Its Use to Add 蚂蚁对零符号的习得及其在加法中的应用
Pub Date : 2020-12-02 DOI: 10.5539/ijb.v12n4p1
M. Cammaerts, R. Cammaerts
It has been previously shown that workers of the ant Myrmica sabuleti can add up numbers of elements when seeing the result of the operation during training, can acquire multiple numerical symbolisms and can make additions using learned numeric symbols. They have also been shown to acquire the notion of zero through experiences. Here, we examined if these ants can associate a symbol with zero and can use this symbol to add. Working on four colonies, we found that the ants acquired a numerical symbolism for zero, and that they used this acquisition for making the additions 1 + 0, 2 + 0, 3 + 0, 4 + 0. This ability simply resulted from operative conditioning and from the ants’ capability in adding up two amounts sighted side by side. The latter imperative condition shows that ants’ numerical cognitive faculties are at a lower level than that observed in honeybees, in some birds and monkeys.
先前有研究表明,在训练过程中,工蚁Myrmica sabuleti在看到运算结果时可以将元素的数量相加,可以获得多个数字符号,并可以使用所学的数字符号进行加法。他们也被证明通过经验获得了零的概念。在这里,我们检查了这些蚂蚁是否可以将符号与零联系起来,并可以使用这个符号进行加法。在四个蚁群中,我们发现蚂蚁获得了零的数字符号,并且它们使用这种获取来进行加法1 + 0,2 + 0,3 + 0,4 + 0。这种能力仅仅来自于操作条件,以及蚂蚁将并排看到的两个数字相加的能力。后一种必要条件表明蚂蚁的数字认知能力低于蜜蜂、某些鸟类和猴子。
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引用次数: 1
Validation of a HPLC Method for the Determination of 4-Hydroxy-3-methoxycinnamyl Alcohol in Extracts Obtained from Stachyurus Chinensis Franeh 高效液相色谱法测定中国石竹提取物中4-羟基-3-甲氧基肉桂醇含量的验证
Pub Date : 2020-09-30 DOI: 10.5539/IJB.V12N3P40
Jing Ji, Jie Cao, Jianming Cheng
Stachyuri medulla helwingiae medulla is a wild used genus in clinic, especially in promoting lactation. Most study confirmed it’s evidently rich in polysaccharide. In this study, a HPLC method for the quantification of Coniferyl alcohol (4-Hydroxy-3-methoxycinnamyl alcohol) was validated. The results showed that the HPLC method was linear (0.01-0.16mg/ml) with good accuracy, precision and robustness. The content of Coniferyl alcohol in different formula granule ranged from 0.091-0.163mg/g. Thus, the method might be used for the standardization of the extracts based on the Coniferyl alcohol marker.
延髓Stachyuri medulla helwingiae medulla是临床上常用的野生属,尤其具有催乳作用。大多数研究证实它明显富含多糖。本研究建立了测定松柏醇(4-羟基-3-甲氧基肉桂醇)含量的高效液相色谱方法。结果表明,该方法线性(0.01 ~ 0.16mg/ml),准确度、精密度和鲁棒性好。不同配方颗粒中针叶醇的含量为0.091 ~ 0.163mg/g。因此,该方法可作为松柏醇标记物的标准化方法。
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引用次数: 0
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International Journal of Biology
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