Artemisia, one of the larger genera in the family Asteraceae, comprises from 200 to more than 500 taxa at the specific or subspecific level. Artemisia herba-alba Asso (Shih) is grown in North Africa and certain parts of Asia and Middle East. It is one of the most widely used plants in the Algerian folk medicine. The antioxidant and free radical scavenging activities of the methanolic extracted materials were tested together with their antibacterial effects against isolated bacterial strains causing food poisoning. In summary, A. herba-alba Asso represent a good phenolic and flavonoid content (161, 64 mg/g and 16,83mg/g, respectively) in comparison with T. capitatus L which represent a phenolic content of 131, 48 mg/g and 14, 96 of flavonoid content. The results shows also that the methanolic extracts of the two plants possess a strong antioxidant (DPPH, FRAP assays) and antibacterial activities, which supports their ethnopharmacological use and A. herba alba represent the highest values (IC 50: 2, 35 mg/ml and OD 1, 13 at 700nm). Our results show the interest of A. herba-alba Asso and T. capitatus L, among other medicinal plants, in search of new chemo-preventive agents against biofilm and planktonic growth of food spoilage pathogens. Further studies are envisaged to target the most interesting molecules responsible for these activities. It is concluded that organic extracts from Artemisia herba alba Asso, exert strong antioxidant activities which are related to their polyphenol contents.
{"title":"Effect of phenolic extracts of Algerian medicinal plants on the bacterial growth and adherence of Staphylococcus aureus and Bacillus cereus pathogens responsible of food poisoning.","authors":"I. Belkacem, Rebai Ouafa, D. Rachid","doi":"10.5138/09750185.2018","DOIUrl":"https://doi.org/10.5138/09750185.2018","url":null,"abstract":"Artemisia, one of the larger genera in the family Asteraceae, comprises from 200 to more than 500 taxa at the specific or subspecific level. Artemisia herba-alba Asso (Shih) is grown in North Africa and certain parts of Asia and Middle East. It is one of the most widely used plants in the Algerian folk medicine. The antioxidant and free radical scavenging activities of the methanolic extracted materials were tested together with their antibacterial effects against isolated bacterial strains causing food poisoning. In summary, A. herba-alba Asso represent a good phenolic and flavonoid content (161, 64 mg/g and 16,83mg/g, respectively) in comparison with T. capitatus L which represent a phenolic content of 131, 48 mg/g and 14, 96 of flavonoid content. The results shows also that the methanolic extracts of the two plants possess a strong antioxidant (DPPH, FRAP assays) and antibacterial activities, which supports their ethnopharmacological use and A. herba alba represent the highest values (IC 50: 2, 35 mg/ml and OD 1, 13 at 700nm). Our results show the interest of A. herba-alba Asso and T. capitatus L, among other medicinal plants, in search of new chemo-preventive agents against biofilm and planktonic growth of food spoilage pathogens. Further studies are envisaged to target the most interesting molecules responsible for these activities. It is concluded that organic extracts from Artemisia herba alba Asso, exert strong antioxidant activities which are related to their polyphenol contents.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"59 2 1","pages":"479-489"},"PeriodicalIF":0.0,"publicationDate":"2017-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79261510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present study was carried out in Sangina valley of Batkhela, Malakand District, Khyber Pakhtunkhwa Pakistan. The aim of the current work was the documentation of plants utilized by the local inhabitants for different medicinal purposes. Majority of cases utilized same plant species in several ways for distinct remedies. After collecting informations through an open-ended questionnaire, totality of 46 plant species used for different medicinal purposes were recorded in the study area belonging to 31 families. Family Lamiaceae ranked as top by contributing 10.86% to the total documented medicinal plant species in the studied area. Family Euphorbiaceae and family Solanacaea shares individually 8.69% and 6.52% to the total, followed by Family Mimosaceae, Asteraceae, Moraceae and family Polygonaceae each having a contribution of 4.34%. Of total 46 reported plant species, 12 plant species were used as diuretic, 8 species were found good as tonic, 7 plant species were significant in curing of cough and 6 plant species were used for remedy of stomach pain. 5 plant species each were used for curing of fever, diarrhea and dysentery.
{"title":"Traditional uses of wild medicinally important plants of Batkhela, kpk, Pakistan","authors":"F. Ali, I. Khan, S. Ullah, M. Ihsan, A. Khan","doi":"10.5138/09750185.2092","DOIUrl":"https://doi.org/10.5138/09750185.2092","url":null,"abstract":"The present study was carried out in Sangina valley of Batkhela, Malakand District, Khyber Pakhtunkhwa Pakistan. The aim of the current work was the documentation of plants utilized by the local inhabitants for different medicinal purposes. Majority of cases utilized same plant species in several ways for distinct remedies. After collecting informations through an open-ended questionnaire, totality of 46 plant species used for different medicinal purposes were recorded in the study area belonging to 31 families. Family Lamiaceae ranked as top by contributing 10.86% to the total documented medicinal plant species in the studied area. Family Euphorbiaceae and family Solanacaea shares individually 8.69% and 6.52% to the total, followed by Family Mimosaceae, Asteraceae, Moraceae and family Polygonaceae each having a contribution of 4.34%. Of total 46 reported plant species, 12 plant species were used as diuretic, 8 species were found good as tonic, 7 plant species were significant in curing of cough and 6 plant species were used for remedy of stomach pain. 5 plant species each were used for curing of fever, diarrhea and dysentery.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"12 1","pages":"471-478"},"PeriodicalIF":0.0,"publicationDate":"2017-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83693145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: To investigate the antidiabetic and antioxidative potentiality of ethyl-acetate fraction of methanolic extract of Camellia sinensis (Green tea) leaves in streptozotocin induced diabetic rat. Methods: Streptozotocin induced diabetic state was confirmed by increased level of fasting blood glucose, decreased level of serum insulin along with inhibition in carbohydrate metabolomics. Oxidative stress was assessed by measuring antioxidative enzyme activities of hepatic and skeleto-muscular tissue. Hepatic Hexokinase-I , pro-apoptotic Bax and anti-apoptotic Bcl-2 gene expression patterns were noted by qRT-PCR technique. Results: After treatment with ethyl-acetate fraction of methanolic extract of Camellia sinensis (Green tea) leaves at a dose of 100 mg/kg body weight/day to diabetic rats for 28 days, a significant (p < 0.05) recovery was noted in fasting blood glucose level, serum insulin level along with activities of carbohydrate metabolic enzymes in hepatic tissue in respect to the vehicle treated diabetic group. This fraction also resulted a significant (p < 0.05) recovery in the activities of antioxidative enzymes in hepatic and skeleto - mascular tissue. In streptozotocin induced diabetic rat the low level of expression of Hexokinase-I , anti-apoptotic Bcl-2 and high level of expression of pro-apoptotic Bax gene were observed in hepatic tissue in respect to vehicle treated control. There were recovered significantly after the treatment with the said fraction. Conclusion: From the results, it may be concluded that ethyl-acetate fraction of methanolic extract of leaves of C. sinensis has a promising anti-diabetic and antioxidative activities for the management of streptozotocin induced diabetic state.
{"title":"Evaluation of antidiabetic and antioxidative efficacy of ethyl acetate fraction of methanolic extract of Camellia sinensis (green tea) leaves in streptozotocin induced diabetic albino rat","authors":"B. Biswas, Barnali Das, Kishalay Jana, D. Ghosh","doi":"10.5138/09750185.2025","DOIUrl":"https://doi.org/10.5138/09750185.2025","url":null,"abstract":"Objective: To investigate the antidiabetic and antioxidative potentiality of ethyl-acetate fraction of methanolic extract of Camellia sinensis (Green tea) leaves in streptozotocin induced diabetic rat. Methods: Streptozotocin induced diabetic state was confirmed by increased level of fasting blood glucose, decreased level of serum insulin along with inhibition in carbohydrate metabolomics. Oxidative stress was assessed by measuring antioxidative enzyme activities of hepatic and skeleto-muscular tissue. Hepatic Hexokinase-I , pro-apoptotic Bax and anti-apoptotic Bcl-2 gene expression patterns were noted by qRT-PCR technique. Results: After treatment with ethyl-acetate fraction of methanolic extract of Camellia sinensis (Green tea) leaves at a dose of 100 mg/kg body weight/day to diabetic rats for 28 days, a significant (p < 0.05) recovery was noted in fasting blood glucose level, serum insulin level along with activities of carbohydrate metabolic enzymes in hepatic tissue in respect to the vehicle treated diabetic group. This fraction also resulted a significant (p < 0.05) recovery in the activities of antioxidative enzymes in hepatic and skeleto - mascular tissue. In streptozotocin induced diabetic rat the low level of expression of Hexokinase-I , anti-apoptotic Bcl-2 and high level of expression of pro-apoptotic Bax gene were observed in hepatic tissue in respect to vehicle treated control. There were recovered significantly after the treatment with the said fraction. Conclusion: From the results, it may be concluded that ethyl-acetate fraction of methanolic extract of leaves of C. sinensis has a promising anti-diabetic and antioxidative activities for the management of streptozotocin induced diabetic state.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"47 1","pages":"305-314"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75651657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim of the study: The purpose of this study was to investigate the possibility of garlic extract as a feed additive to prevent and treat Salmonella infection. Methods: Garlic extracts were prepared from fresh crushed garlic and dissolved in different concentrations of ethanol. Garlic extract ( Allium sativum ) was analyzed by using gas chromatography-mass spectrometry (GC-MS). To examine biological activity, the antimicrobial activity test was carried out by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Salmonella typhimurium. Antioxidant activity of garlic extracts was examined by ABTS radical scavenging assay Results and discussion : In the GC-MS analysis, allyl trisulfide, 2-hydroxy-gamma-butyrolactone, 1, 3-dihydroxyacetne dimer, propanoic acid, 2-propone were confirmed as predominant components of garlic extract. the MIC was 10 mg/mL in all extracts. However, antioxidant effect was highest in 20 mg/mL of 100% ethanol garlic extract (82.1%). The amount of IC₅₀ (50% inhibitory concentration) was measured at 1.6 mg/mL. Conclusion: 75% ethanol garlic extract was the most efficient in considering the recovery rate, antimicrobial and antioxidant activities among the various extracts. Based on the above biological results, we could confirm this possibility as a feed additive for anti-salmonella infection.
{"title":"GC/MS analysis, antimicrobial and Antioxidant Effect of Ethanol Garlic Extract","authors":"N. Park, Hyejin Jang, S. J. Lee, Seung-Chun Park","doi":"10.5138/09750185.2087","DOIUrl":"https://doi.org/10.5138/09750185.2087","url":null,"abstract":"Aim of the study: The purpose of this study was to investigate the possibility of garlic extract as a feed additive to prevent and treat Salmonella infection. Methods: Garlic extracts were prepared from fresh crushed garlic and dissolved in different concentrations of ethanol. Garlic extract ( Allium sativum ) was analyzed by using gas chromatography-mass spectrometry (GC-MS). To examine biological activity, the antimicrobial activity test was carried out by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Salmonella typhimurium. Antioxidant activity of garlic extracts was examined by ABTS radical scavenging assay Results and discussion : In the GC-MS analysis, allyl trisulfide, 2-hydroxy-gamma-butyrolactone, 1, 3-dihydroxyacetne dimer, propanoic acid, 2-propone were confirmed as predominant components of garlic extract. the MIC was 10 mg/mL in all extracts. However, antioxidant effect was highest in 20 mg/mL of 100% ethanol garlic extract (82.1%). The amount of IC₅₀ (50% inhibitory concentration) was measured at 1.6 mg/mL. Conclusion: 75% ethanol garlic extract was the most efficient in considering the recovery rate, antimicrobial and antioxidant activities among the various extracts. Based on the above biological results, we could confirm this possibility as a feed additive for anti-salmonella infection.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"87 1","pages":"324-331"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85625117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iftikhar Ali, H. Hussain, H. Batool, A. Dad, G. Raza, A. Falodun, R. Ullah, V. Ahmad
Majority of the livestock holders in different areas of Central Karakoram National Park (CKNP) region in Gilgit-Baltistan lack the modern veterinary healthcare facilities. The local community mostly depends upon the traditional floral therapeutic veterinary practices for the cure of various livestock diseases. The lack of the ethnoveterinary surveys in the CKNP region results in loss in the scientific documentation of the traditional information and practices about the livestock healthcare. The present study deals with the documentation of the livestock diseases and the ethnoveterinary herbal and non-herbal therapeutic practices by the livestock holders in CKNP region. The study was conducted from March 2012 to March 2013 in the valleys of CKNP region. The ethnoveterinary data was collected through the semi-structured questionnaires and the techniques of interviews and field visits. And the productive interaction provided an enabling background for the successful Rapid Rural Appraisal and Participatory Rural Appraisal research. Total 156 local community members were interviewed in Thallay, Khaplu, Hushe and Shigar valleys. The survey showed the prevalence of the most common diseases e.g. FMD, intestinal problems etc. Among the 51 medicinal plants, the Asteraceae family exhibited the highest number of genera with the therapeutic species. The parts of the plants that were most frequently used for the therapeutic purposes were the leaves (55%), seed (15%) etc. The present study contributed to the documentation of the medicinal plants used in the ethnoveterinary practices in valleys in CKNP region, Gilgit-Baltistan.
{"title":"Documentation of Ethnoveterinary Practices in the CKNP Region, Gilgit-Baltistan","authors":"Iftikhar Ali, H. Hussain, H. Batool, A. Dad, G. Raza, A. Falodun, R. Ullah, V. Ahmad","doi":"10.5138/09750185.1898","DOIUrl":"https://doi.org/10.5138/09750185.1898","url":null,"abstract":"Majority of the livestock holders in different areas of Central Karakoram National Park (CKNP) region in Gilgit-Baltistan lack the modern veterinary healthcare facilities. The local community mostly depends upon the traditional floral therapeutic veterinary practices for the cure of various livestock diseases. The lack of the ethnoveterinary surveys in the CKNP region results in loss in the scientific documentation of the traditional information and practices about the livestock healthcare. The present study deals with the documentation of the livestock diseases and the ethnoveterinary herbal and non-herbal therapeutic practices by the livestock holders in CKNP region. The study was conducted from March 2012 to March 2013 in the valleys of CKNP region. The ethnoveterinary data was collected through the semi-structured questionnaires and the techniques of interviews and field visits. And the productive interaction provided an enabling background for the successful Rapid Rural Appraisal and Participatory Rural Appraisal research. Total 156 local community members were interviewed in Thallay, Khaplu, Hushe and Shigar valleys. The survey showed the prevalence of the most common diseases e.g. FMD, intestinal problems etc. Among the 51 medicinal plants, the Asteraceae family exhibited the highest number of genera with the therapeutic species. The parts of the plants that were most frequently used for the therapeutic purposes were the leaves (55%), seed (15%) etc. The present study contributed to the documentation of the medicinal plants used in the ethnoveterinary practices in valleys in CKNP region, Gilgit-Baltistan.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"35 1","pages":"223-240"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82083679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iulia Antioch, A. Ciobica, M. Compaoré, A. Hilou, M. Kiendrebeogo, H. Foyet, S. Guenné
Schizophrenia is an extremely complex psychiatric disease where perception of pain is altered, varying from abolition to lack of any kind of changes compared to normal controls and even hypersensitivity. In this way, the hypothesis of amending schizophrenia through pain therapy enhanced the importance of pain medication. But managing pain phenomenon in schizophrenia has large and unknown implications. Nevertheless, pharmacological interactions between the medications for these two entities are unknown and most likely would have a lot of side-effects and therefore ethnopharmacological methods became once again an interesting option. Traditional medicine wisdom was followed in the pursuit of finding connections between ancient knowledge and current scientific proven facts. To our best of knowledge, this is the first time when pain, plants and schizophrenia are discussed together. In this way, it seems that by replacing fully synthesized chemical products, the risk of side-effects decreases. Also, it appears that some plants besides treating pain may have curing effects on the psychotic activities in schizophrenia. Therefore, through this mini-review we emphasized on the advantages of the ethonopharmacological approaches in pain conditions in the context of schizophrenia, but also highlighted some cases of inappropriate usage of plants in traditional therapy.
{"title":"Phytotherapeutical implications in pain perception - focusing on schizophrenia","authors":"Iulia Antioch, A. Ciobica, M. Compaoré, A. Hilou, M. Kiendrebeogo, H. Foyet, S. Guenné","doi":"10.5138/09750185.1891","DOIUrl":"https://doi.org/10.5138/09750185.1891","url":null,"abstract":"Schizophrenia is an extremely complex psychiatric disease where perception of pain is altered, varying from abolition to lack of any kind of changes compared to normal controls and even hypersensitivity. In this way, the hypothesis of amending schizophrenia through pain therapy enhanced the importance of pain medication. But managing pain phenomenon in schizophrenia has large and unknown implications. Nevertheless, pharmacological interactions between the medications for these two entities are unknown and most likely would have a lot of side-effects and therefore ethnopharmacological methods became once again an interesting option. Traditional medicine wisdom was followed in the pursuit of finding connections between ancient knowledge and current scientific proven facts. To our best of knowledge, this is the first time when pain, plants and schizophrenia are discussed together. In this way, it seems that by replacing fully synthesized chemical products, the risk of side-effects decreases. Also, it appears that some plants besides treating pain may have curing effects on the psychotic activities in schizophrenia. Therefore, through this mini-review we emphasized on the advantages of the ethonopharmacological approaches in pain conditions in the context of schizophrenia, but also highlighted some cases of inappropriate usage of plants in traditional therapy.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"21 3 1","pages":"167-180"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83954441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Developing traditional medicine in the field of hepatology and nephrology research is the key to pharmacology. This study demonstrates the mechanism of hepatoprotective and renal protective activity of Ficus dalhousiae Miq. (Moraceae) methanolic leaf extract on carbon tetrachloride induced hepatic and renal toxicity. Shade dried powder was subjected to shoxlet extraction with methanol and assessed for hepatoprotective and renal protective activities. Hepatotoxicity and renal toxicity were induced in rats by single oral dose of CCl 4 diluted with olive oil (1:1 v/v; ml/kg body weight) after pretreatment of methanolic extract for seven days. Sixteen hrs after CCl 4 administration, rats were sacrificed and biochemical markers like Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) estimated followed by the measurement of liver and kidney cytosolic antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and reactive oxygen species (ROS). The data were analysed by one way analysis of variance (ANOVA). The extract at the doses of 150 and mg/kg b.w. significantly reduces elevated levels of ALT, AST, ALP and LDH. The extracts also showed significant increase in the reduced level of SOD, CAT and GSH. The ROS activity also found down regulated. The activity of methanolic extracts were comparable with the standard Silymarin. These findings not only showed potential hepatoprotective and renal protective activities of Ficus dalhousiae but also manifested by restoring antioxidant enzymes. With this pilot study we can justify the medicinal importance of this plant.
{"title":"Ameliorative effect of Ficus dalhousiae Miq. (Moraceae) methanolic leaf extract on carbon tetrachloride induced hepatic and renal toxicity","authors":"S. Kujur, K. Sunil, Anand Barapatre, N. Geetha","doi":"10.5138/09750185.1992","DOIUrl":"https://doi.org/10.5138/09750185.1992","url":null,"abstract":"Developing traditional medicine in the field of hepatology and nephrology research is the key to pharmacology. This study demonstrates the mechanism of hepatoprotective and renal protective activity of Ficus dalhousiae Miq. (Moraceae) methanolic leaf extract on carbon tetrachloride induced hepatic and renal toxicity. Shade dried powder was subjected to shoxlet extraction with methanol and assessed for hepatoprotective and renal protective activities. Hepatotoxicity and renal toxicity were induced in rats by single oral dose of CCl 4 diluted with olive oil (1:1 v/v; ml/kg body weight) after pretreatment of methanolic extract for seven days. Sixteen hrs after CCl 4 administration, rats were sacrificed and biochemical markers like Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) estimated followed by the measurement of liver and kidney cytosolic antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and reactive oxygen species (ROS). The data were analysed by one way analysis of variance (ANOVA). The extract at the doses of 150 and mg/kg b.w. significantly reduces elevated levels of ALT, AST, ALP and LDH. The extracts also showed significant increase in the reduced level of SOD, CAT and GSH. The ROS activity also found down regulated. The activity of methanolic extracts were comparable with the standard Silymarin. These findings not only showed potential hepatoprotective and renal protective activities of Ficus dalhousiae but also manifested by restoring antioxidant enzymes. With this pilot study we can justify the medicinal importance of this plant.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"27 1","pages":"332-341"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73381218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Assessment of antioxidant activity was imperative in the screening of medicinal plants for potential health benefits. In present study methanol extract of Polygonum glabrum (polygonaceae) was screened for its in vitro antioxidant activity using biologically relevant methodologies which scavenge radicals such as 1,1 diphenyl 2 picryl hydrazyl (DPPH), nitric oxide, hydrogen peroxide, hydroxyl, superoxide anion and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). Total reducing ability by conversion of ferric (III) to ferrous (II) and molybdenum (VI) to molybdenum (V), metal ion chelating capacity and anti lipid peroxidation activities were also examined. The antioxidant ability of Polygonum glabrum whole plant extract was found to be in a dose dependent manner. The IC 50 values for scavenging of DPPH ● and ABTS ●+ free radicals were 13.18 μg/ml and 20.46 μg/ml. For scavenging of nitric oxide, hydrogen peroxide, hydroxyl and superoxide anion radicals, the IC 50 values were found to be 80.22 μg/ml, 33.06 μg/ml, 52.26μg/ml and 36.98 μg/ml respectively. Further, addition of 120μg/ml of plant extract to the reaction mixture produced 50% lipid peroxidation inhibition activity. Commercial antioxidants such as vitamin E, quercetin, butylated hydroxytoluene and ascorbic acid were used as reference compounds. The strong antioxidant activity of Polygonum glabrum may be credited to the presence of triterpenes [beta-hydroxyfriedalanol], phenols [3-hydroxy-5-methoxystilbene], flavonoids [pinocembrin and pinocembrin-5-methylether], steroids [sitosterol - (6-O-palmitoyl)- 3-O-β-D glucopyranoside and sitosterol-3-O-β-D glucopyranoside], sesqueterpenes [2,3-dihydroxy isodrimeninol] and pigments etc in methanol extract.
{"title":"In Vitro antioxidant activity of Polygonum Glabrum","authors":"R. Rajan, I. Ramya","doi":"10.5138/09750185.2049","DOIUrl":"https://doi.org/10.5138/09750185.2049","url":null,"abstract":"Assessment of antioxidant activity was imperative in the screening of medicinal plants for potential health benefits. In present study methanol extract of Polygonum glabrum (polygonaceae) was screened for its in vitro antioxidant activity using biologically relevant methodologies which scavenge radicals such as 1,1 diphenyl 2 picryl hydrazyl (DPPH), nitric oxide, hydrogen peroxide, hydroxyl, superoxide anion and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS). Total reducing ability by conversion of ferric (III) to ferrous (II) and molybdenum (VI) to molybdenum (V), metal ion chelating capacity and anti lipid peroxidation activities were also examined. The antioxidant ability of Polygonum glabrum whole plant extract was found to be in a dose dependent manner. The IC 50 values for scavenging of DPPH ● and ABTS ●+ free radicals were 13.18 μg/ml and 20.46 μg/ml. For scavenging of nitric oxide, hydrogen peroxide, hydroxyl and superoxide anion radicals, the IC 50 values were found to be 80.22 μg/ml, 33.06 μg/ml, 52.26μg/ml and 36.98 μg/ml respectively. Further, addition of 120μg/ml of plant extract to the reaction mixture produced 50% lipid peroxidation inhibition activity. Commercial antioxidants such as vitamin E, quercetin, butylated hydroxytoluene and ascorbic acid were used as reference compounds. The strong antioxidant activity of Polygonum glabrum may be credited to the presence of triterpenes [beta-hydroxyfriedalanol], phenols [3-hydroxy-5-methoxystilbene], flavonoids [pinocembrin and pinocembrin-5-methylether], steroids [sitosterol - (6-O-palmitoyl)- 3-O-β-D glucopyranoside and sitosterol-3-O-β-D glucopyranoside], sesqueterpenes [2,3-dihydroxy isodrimeninol] and pigments etc in methanol extract.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"564 1","pages":"351-363"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85143179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Florence, D. S. J. Philippe, D. J. Hubert, Gounoue Kamkumo Raceline, D. Désiré, Kamtchouing Pierre, D. Théophile
Hibiscus noldea leaves-stems aqueous extract is used in Cameroonian traditional medecine to manage diabetes. To investigate the preventive effect of Hibiscus noldea aqueous extract on dexamethasone-induced insulin resistance, the animals received one of the following treatments: distilled water (10 mL/kg), metformine (200 mg/kg), or H. noldea (100 or 200 mg/kg) concomitantly with dexamethasone (0.5 mg/kg, ip) for ten days. Body weight was evaluated daily and blood glucose levels were measured. At the end of experiment, insulin sensitivity test was performed and lipid profile, transaminases Aspartate amino transferase, Alanin amino transferase, malondialdehyde, superoxide dismutase, catalase, and reduced glutathione were evaluated. Histological analysis of the liver was investigated to estimate glycogen content using Periodic Acid Schiff coloration and histomorphometry of pancreatic islets area was performed. The administration of dexamethasone during ten days induced body weight loss, hyperglycaemia, insulinresistance, an imbalance in lipid profile, an increase in transaminases and oxidative stress. Dexamethasone treatment also induced an increase in the pancreatic islets area and depletion in the levels of hepatic glycogen. Concomitant administration of dexamethasone and the aqueous plant extract prevented the rise in blood glucose levels, reduced insulinresistance, improved lipid profile and oxidative status. The aqueous extract of H. noldea prevented the use of glycogen storage and the increase in pancreatic islet area in dose dependent manner. Conclusion: The stem leaves aqueous extract from Hibiscus noldea have the ability to reduce insulinresistance via its antihyperglycaemic, hypolipidemic and antioxidant activities. These results justify the use of this extract in the management of diabetic state.
{"title":"Hibiscus noldea (Malvaceae) Aqueous Extract Prevents Insulin Resistance and Protects Pancreatic Islets From Dexamethasone Damages in Rat.","authors":"N. Florence, D. S. J. Philippe, D. J. Hubert, Gounoue Kamkumo Raceline, D. Désiré, Kamtchouing Pierre, D. Théophile","doi":"10.5138/09750185.2036","DOIUrl":"https://doi.org/10.5138/09750185.2036","url":null,"abstract":"Hibiscus noldea leaves-stems aqueous extract is used in Cameroonian traditional medecine to manage diabetes. To investigate the preventive effect of Hibiscus noldea aqueous extract on dexamethasone-induced insulin resistance, the animals received one of the following treatments: distilled water (10 mL/kg), metformine (200 mg/kg), or H. noldea (100 or 200 mg/kg) concomitantly with dexamethasone (0.5 mg/kg, ip) for ten days. Body weight was evaluated daily and blood glucose levels were measured. At the end of experiment, insulin sensitivity test was performed and lipid profile, transaminases Aspartate amino transferase, Alanin amino transferase, malondialdehyde, superoxide dismutase, catalase, and reduced glutathione were evaluated. Histological analysis of the liver was investigated to estimate glycogen content using Periodic Acid Schiff coloration and histomorphometry of pancreatic islets area was performed. The administration of dexamethasone during ten days induced body weight loss, hyperglycaemia, insulinresistance, an imbalance in lipid profile, an increase in transaminases and oxidative stress. Dexamethasone treatment also induced an increase in the pancreatic islets area and depletion in the levels of hepatic glycogen. Concomitant administration of dexamethasone and the aqueous plant extract prevented the rise in blood glucose levels, reduced insulinresistance, improved lipid profile and oxidative status. The aqueous extract of H. noldea prevented the use of glycogen storage and the increase in pancreatic islet area in dose dependent manner. Conclusion: The stem leaves aqueous extract from Hibiscus noldea have the ability to reduce insulinresistance via its antihyperglycaemic, hypolipidemic and antioxidant activities. These results justify the use of this extract in the management of diabetic state.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"49 1","pages":"267-274"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84889312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phalloidin is a cyclic heptapeptide containing cysteine amino acids. The toxicity of phallodin is attributed to the sulfur atom of the sulfur in the indole ring of the molecule and it is responsible for acute gastroenteritis occurring during the initial period of poisoning. Pelargonium sidoides, reinforcing the immune system, antiviral, has antibacterial properties as well as antioxidant properties.The aim of this study is to investigate the protective role of Pelargonium sidoides and Curcumin against mushroom poisoning. 28 Wistar albino rats were divided into four groups. Group I; Along the study, 0.2 ml saline was administered intraperitoneally to the rats .Group II (phalloidin, 0.5 mg / kg) were administered for 5 days of study. Group III : From the beginning to the end of the study, 0.5 mg / kg phalloidin i.p.,0,2 ml/kg pelargonium sidoides were administered orally. Group IV : From the beginning to the end of the study, 0.5 mg / kg phalloidin intraperitoneally,0.2 ml/kg Curcumin were administered orally.At the end of the study, the rats were sacrificed. The blood of the rats was taken. In serum Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px), Paraxonase (PON), Arylesterase (ARE) ,Adenosine Deaminase (ADA) , Xhanthine oxidase (XO),Protein Karbonyl (PC), Malondialdehyde(MDA), Nitric oxide (NO), were measured by spectrophotometry. In Group II, MDA, , PC levels and XO activity increased significantly compared to Group I. (p<0.05).PS and Curcumin treatment ameliorated some enzyme levels (SOD, GSH-px, PON, ADA ) in serum with phalloidin induced rats. Values of Group III approached Group I. Phalloidin increases toxic ROS. Pelargonium sidoides and Curcumin are antioxidant, antitoxic. And They such as silibin can protective against mushroom poisoning.
{"title":"Role of protective Pelargonium Sidoides root extract and Curcumin on Mushroom poisoning: An Experimental study in rats","authors":"V. Unsal, E. Kurutaş, M. Güngör, Aksan M Emrah","doi":"10.5138/09750185.2050","DOIUrl":"https://doi.org/10.5138/09750185.2050","url":null,"abstract":"Phalloidin is a cyclic heptapeptide containing cysteine amino acids. The toxicity of phallodin is attributed to the sulfur atom of the sulfur in the indole ring of the molecule and it is responsible for acute gastroenteritis occurring during the initial period of poisoning. Pelargonium sidoides, reinforcing the immune system, antiviral, has antibacterial properties as well as antioxidant properties.The aim of this study is to investigate the protective role of Pelargonium sidoides and Curcumin against mushroom poisoning. 28 Wistar albino rats were divided into four groups. Group I; Along the study, 0.2 ml saline was administered intraperitoneally to the rats .Group II (phalloidin, 0.5 mg / kg) were administered for 5 days of study. Group III : From the beginning to the end of the study, 0.5 mg / kg phalloidin i.p.,0,2 ml/kg pelargonium sidoides were administered orally. Group IV : From the beginning to the end of the study, 0.5 mg / kg phalloidin intraperitoneally,0.2 ml/kg Curcumin were administered orally.At the end of the study, the rats were sacrificed. The blood of the rats was taken. In serum Superoxide dismutase (SOD), Glutathione peroxidase (GSH-Px), Paraxonase (PON), Arylesterase (ARE) ,Adenosine Deaminase (ADA) , Xhanthine oxidase (XO),Protein Karbonyl (PC), Malondialdehyde(MDA), Nitric oxide (NO), were measured by spectrophotometry. In Group II, MDA, , PC levels and XO activity increased significantly compared to Group I. (p<0.05).PS and Curcumin treatment ameliorated some enzyme levels (SOD, GSH-px, PON, ADA ) in serum with phalloidin induced rats. Values of Group III approached Group I. Phalloidin increases toxic ROS. Pelargonium sidoides and Curcumin are antioxidant, antitoxic. And They such as silibin can protective against mushroom poisoning.","PeriodicalId":14199,"journal":{"name":"International Journal of Phytomedicine","volume":"4 24 1","pages":"261-266"},"PeriodicalIF":0.0,"publicationDate":"2017-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90297872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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