J. Alander, V. Bochko, B. Martinkauppi, S. Saranwong, Timo Mantere
This paper is a review of optical methods for online nondestructive food quality monitoring. The key spectral areas are the visual and near-infrared wavelengths. We have collected the information of over 260 papers published mainly during the last 20 years. Many of them use an analysis method called chemometrics which is shortly described in the paper. The main goal of this paper is to provide a general view of work done according to different FAO food classes. Hopefully using optical VIS/NIR spectroscopy gives an idea of how to better meet market and consumer needs for high-quality food stuff.
{"title":"A Review of Optical Nondestructive Visual and Near-Infrared Methods for Food Quality and Safety","authors":"J. Alander, V. Bochko, B. Martinkauppi, S. Saranwong, Timo Mantere","doi":"10.1155/2013/341402","DOIUrl":"https://doi.org/10.1155/2013/341402","url":null,"abstract":"This paper is a review of optical methods for online nondestructive food quality monitoring. The key spectral areas are the visual and near-infrared wavelengths. We have collected the information of over 260 papers published mainly during the last 20 years. Many of them use an analysis method called chemometrics which is shortly described in the paper. The main goal of this paper is to provide a general view of work done according to different FAO food classes. Hopefully using optical VIS/NIR spectroscopy gives an idea of how to better meet market and consumer needs for high-quality food stuff.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"41 1","pages":"1-36"},"PeriodicalIF":0.0,"publicationDate":"2013-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86411786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Surface-enhanced Raman scattering (SERS) was discovered in 1974 and impacted Raman spectroscopy and surface science. Although SERS has not been developed to be an applicable detection tool so far, nanotechnology has promoted its development in recent decades. The traditional SERS substrates, such as silver electrode, metal island film, and silver colloid, cannot be applied because of their enhancement factor or stability, but newly developed substrates, such as electrochemical deposition surface, Ag porous film, and surface-confined colloids, have better sensitivity and stability. Surface enhanced Raman scattering is applied in other fields such as detection of chemical pollutant, biomolecules, DNA, bacteria, and so forth. In this paper, the development of nanofabrication and application of surface-enhanced Ramans scattering substrate are discussed.
{"title":"The Nanofabrication and Application of Substrates for Surface-Enhanced Raman Scattering","authors":"Xian Zhang, Qin Zhou, Yu Huang, Zhengcao Li, Zhengjun Zhang","doi":"10.1155/2012/350684","DOIUrl":"https://doi.org/10.1155/2012/350684","url":null,"abstract":"Surface-enhanced Raman scattering (SERS) was discovered in 1974 and impacted Raman spectroscopy and surface science. Although SERS has not been developed to be an applicable detection tool so far, nanotechnology has promoted its development in recent decades. The traditional SERS substrates, such as silver electrode, metal island film, and silver colloid, cannot be applied because of their enhancement factor or stability, but newly developed substrates, such as electrochemical deposition surface, Ag porous film, and surface-confined colloids, have better sensitivity and stability. Surface enhanced Raman scattering is applied in other fields such as detection of chemical pollutant, biomolecules, DNA, bacteria, and so forth. In this paper, the development of nanofabrication and application of surface-enhanced Ramans scattering substrate are discussed.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"15 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2012-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81898469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yao-Fang Hsieh, M. Ou‐Yang, J. Duann, J. Chiou, N. Chang, C. Jan, M. Tsai, Shuen-De Wu, Yung-Jiun Lin, Cheng-chung Lee
This paper develops a novel embedded relay lens microscopic hyperspectral imaging system (ERL-MHSI) with high spectral resolution (nominal spectral resolution of 2.8 nm) and spatial resolution (30 μm × 10 μm) for cancer diagnosis. The ERL-MHSI system has transmittance and fluorescence mode. The transmittance can provide the morphological information for pathological diagnosis, andthe fluorescence of cells or tissue can provide the characteristic signature for identification of normal and abnormal. In this work, the development of the ERL-MHSI system is discussed and the capability of the system is demonstrated by diagnosing early stage oral cancer of twenty mice in vitro. The best sensitivity for identifying normal cells and squamous cell carcinoma (SCC) was 100%. The best specificity for identifying normal cells and SCC was 99%. The best sensitivity for identifying normal cells and dysplasia was 99%. The best specificity for identifying normal cells and dysplasia was 97%. This work also utilizes fractal dimension to analyze the morphological information and find the significant different values between normal and SCC.
{"title":"Development of a Novel Embedded Relay Lens Microscopic Hyperspectral Imaging System for Cancer Diagnosis: Use of the Mice with Oral Cancer to Be the Example","authors":"Yao-Fang Hsieh, M. Ou‐Yang, J. Duann, J. Chiou, N. Chang, C. Jan, M. Tsai, Shuen-De Wu, Yung-Jiun Lin, Cheng-chung Lee","doi":"10.1155/2012/710803","DOIUrl":"https://doi.org/10.1155/2012/710803","url":null,"abstract":"This paper develops a novel embedded relay lens microscopic hyperspectral imaging system (ERL-MHSI) with high spectral resolution (nominal spectral resolution of 2.8 nm) and spatial resolution (30 μm × 10 μm) for cancer diagnosis. The ERL-MHSI system has transmittance and fluorescence mode. The transmittance can provide the morphological information for pathological diagnosis, andthe fluorescence of cells or tissue can provide the characteristic signature for identification of normal and abnormal. In this work, the development of the ERL-MHSI system is discussed and the capability of the system is demonstrated by diagnosing early stage oral cancer of twenty mice in vitro. The best sensitivity for identifying normal cells and squamous cell carcinoma (SCC) was 100%. The best specificity for identifying normal cells and SCC was 99%. The best sensitivity for identifying normal cells and dysplasia was 99%. The best specificity for identifying normal cells and dysplasia was 97%. This work also utilizes fractal dimension to analyze the morphological information and find the significant different values between normal and SCC.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"42 1","pages":"1-13"},"PeriodicalIF":0.0,"publicationDate":"2012-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85121725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We report on a novel and extremely low-cost surface-enhanced Raman spectroscopy (SERS) substrate fabricated depositing gold nanoparticles on common lab filter paper using thermal inkjet technology. The paper-based substrate combines all advantages of other plasmonic structures fabricated by more elaborate techniques with the dynamic flexibility given by the inherent nature of the paper for an efficient sample collection, robustness, and stability. We describe the fabrication, characterization, and SERS activity of our substrate using 2,4,6-trinitrotoluene, 2,4-dinitrotoluene, and 1,3,5-trinitrobenzene as analytes. The paper-based SERS substrates presented a high sensitivity and excellent reproducibility for analytes employed, demonstrating a direct application in forensic science and homeland security.
{"title":"Highly Sensitive Filter Paper Substrate for SERS Trace Explosives Detection","authors":"P. Fierro-Mercado, S. Hernández‐Rivera","doi":"10.1155/2012/716527","DOIUrl":"https://doi.org/10.1155/2012/716527","url":null,"abstract":"We report on a novel and extremely low-cost surface-enhanced Raman spectroscopy (SERS) substrate fabricated depositing gold nanoparticles on common lab filter paper using thermal inkjet technology. The paper-based substrate combines all advantages of other plasmonic structures fabricated by more elaborate techniques with the dynamic flexibility given by the inherent nature of the paper for an efficient sample collection, robustness, and stability. We describe the fabrication, characterization, and SERS activity of our substrate using 2,4,6-trinitrotoluene, 2,4-dinitrotoluene, and 1,3,5-trinitrobenzene as analytes. The paper-based SERS substrates presented a high sensitivity and excellent reproducibility for analytes employed, demonstrating a direct application in forensic science and homeland security.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"14 5 Suppl 3 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2012-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84982847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Zenkevich, T. Blaudeck, A. Milekhin, C. Borczyskowski
We review recent experimental work to utilize the size dependence of the luminescence quenching of colloidal semiconductor quantum dots induced by functionalized porphyrin molecules attached to the surface to describe a photoluminescence (PL) quenching process which is different from usual models of charge transfer (CT) or Foerster resonant energy transfer (FRET). Steady-state and picosecond time-resolved measurements were carried out for nanocomposites based on colloidal CdSe/ZnS and CdSe quantum dots (QDs) of various sizes and surfacely attached tetra-mesopyridyl-substituted porphyrin molecules (“Quantum Dot-Porphyrin” nanocomposites), in toluene at 295 K. It was found that the major part of the observed strong quenching of QD PL in “QD-Porphyrin” nanocomposites can neither be assigned to FRET nor to photoinduced charge transfer between the QD and the chromophore. This PL quenching depends on QD size and shell and is stronger for smaller quantum dots: QD PL quenching rate constants 𝑘𝑞 scale inversely with the QD diameter. Based on the comparison of experimental data and quantum mechanical calculations, it has been concluded that QD PL quenching in “QD-Porphyrin” nanocomposites can be understood in terms of a tunneling of the electron (of the excited electron-hole pair) followed by a (self-) localization of the electron or formation of trap states. The major contribution to PL quenching is found to be proportional to the calculated quantum-confined exciton wave function at the QD surface. Our findings highlight that single functionalized molecules can be considered as one of the probes for the complex interface physics and dynamics of colloidal semiconductor QD.
{"title":"Size-Dependent Non-FRET Photoluminescence Quenching in Nanocomposites Based on Semiconductor Quantum Dots CdSe/ZnS and Functionalized Porphyrin Ligands","authors":"E. Zenkevich, T. Blaudeck, A. Milekhin, C. Borczyskowski","doi":"10.1155/2012/971791","DOIUrl":"https://doi.org/10.1155/2012/971791","url":null,"abstract":"We review recent experimental work to utilize the size dependence of the luminescence quenching of colloidal semiconductor quantum dots induced by functionalized porphyrin molecules attached to the surface to describe a photoluminescence (PL) quenching process which is different from usual models of charge transfer (CT) or Foerster resonant energy transfer (FRET). Steady-state and picosecond time-resolved measurements were carried out for nanocomposites based on colloidal CdSe/ZnS and CdSe quantum dots (QDs) of various sizes and surfacely attached tetra-mesopyridyl-substituted porphyrin molecules (“Quantum Dot-Porphyrin” nanocomposites), in toluene at 295 K. It was found that the major part of the observed strong quenching of QD PL in “QD-Porphyrin” nanocomposites can neither be assigned to FRET nor to photoinduced charge transfer between the QD and the chromophore. This PL quenching depends on QD size and shell and is stronger for smaller quantum dots: QD PL quenching rate constants 𝑘𝑞 scale inversely with the QD diameter. Based on the comparison of experimental data and quantum mechanical calculations, it has been concluded that QD PL quenching in “QD-Porphyrin” nanocomposites can be understood in terms of a tunneling of the electron (of the excited electron-hole pair) followed by a (self-) localization of the electron or formation of trap states. The major contribution to PL quenching is found to be proportional to the calculated quantum-confined exciton wave function at the QD surface. Our findings highlight that single functionalized molecules can be considered as one of the probes for the complex interface physics and dynamics of colloidal semiconductor QD.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"252 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2012-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77531290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Azhniuk, Yu. I. Hutych, V. Lopushansky, Mykola V. Prymak, A. Gomonnai, D. Zahn
A series of samples with quaternary CdS1−x−ySexTey nanocrystals with cations of three types distributed over nanocrystal lattice sites were grown in a borosilicate glass matrix by the diffusion-limited growth technique. Their Raman spectra confirm the three-mode compositional behaviour of CdS1−x−ySexTey phonon spectra. The compositional dependence of the LO phonon frequencies is analysed experimentally for a series of samples containing nanocrystals with x≈y.
{"title":"Raman Spectra of Quaternary CdS1−x−ySexTey Nanocrystals Embedded in Borosilicate Glass","authors":"Y. Azhniuk, Yu. I. Hutych, V. Lopushansky, Mykola V. Prymak, A. Gomonnai, D. Zahn","doi":"10.1155/2012/495896","DOIUrl":"https://doi.org/10.1155/2012/495896","url":null,"abstract":"A series of samples with quaternary CdS1−x−ySexTey nanocrystals with cations of three types distributed over nanocrystal lattice sites were grown in a borosilicate glass matrix by the diffusion-limited growth technique. Their Raman spectra confirm the three-mode compositional behaviour of CdS1−x−ySexTey phonon spectra. The compositional dependence of the LO phonon frequencies is analysed experimentally for a series of samples containing nanocrystals with x≈y.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"39 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2012-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77134354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
We have calculated the vibrational solvent shifts of the fundamental bands of HCl diluted in Ar, Kr, and Xe solutions at different thermodynamic conditions by means of the molecular dynamics technique and a model for the isotropic part of the interaction depending on the vibration. The theoretical vibrational shifts, which were compared with the available experimental data, have been determined by considering both, the usual linear Buckingham terms and the nonlinear anharmonic corrections, and the latter omitted in a previous work for the HCl in Ar and Kr. We have found that the Buckingham contributions dominate the solvent shifts of the fundamental bands of HCl in Ar, Kr, and Xe, although the anharmonic shifts’ present significant greater values than those obtained previously for N2 diluted in liquid Ar and pure liquid N2, both at normal conditions. We have analyzed the solvent shifts influence of the linear and quadratic (in the vibrational coordinate) oscillator-bath interaction terms and also the Dunham intramolecular potential effects on the anharmonic contributions.
{"title":"A Simulation Study of the Fundamental Vibrational Shifts of HCl Diluted in Ar, Kr, and Xe: Anharmonic Corrections Effects","authors":"A. Padilla, J. Pérez","doi":"10.1155/2012/851923","DOIUrl":"https://doi.org/10.1155/2012/851923","url":null,"abstract":"We have calculated the vibrational solvent shifts of the fundamental bands of HCl diluted in Ar, Kr, and Xe solutions at different thermodynamic conditions by means of the molecular dynamics technique and a model for the isotropic part of the interaction depending on the vibration. The theoretical vibrational shifts, which were compared with the available experimental data, have been determined by considering both, the usual linear Buckingham terms and the nonlinear anharmonic corrections, and the latter omitted in a previous work for the HCl in Ar and Kr. We have found that the Buckingham contributions dominate the solvent shifts of the fundamental bands of HCl in Ar, Kr, and Xe, although the anharmonic shifts’ present significant greater values than those obtained previously for N2 diluted in liquid Ar and pure liquid N2, both at normal conditions. We have analyzed the solvent shifts influence of the linear and quadratic (in the vibrational coordinate) oscillator-bath interaction terms and also the Dunham intramolecular potential effects on the anharmonic contributions.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"9 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2012-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86945925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vibrational properties of hydrogenated silicon-rich nitride ( S i N 𝑥 : H ) of various stoichiometry ( 0 . 6 ≤ 𝑥 ≤ 1 . 3 ) and hydrogenated amorphous silicon ( a - S i : H ) films were studied using Raman spectroscopy and Fourier transform infrared spectroscopy. Furnace annealing during 5 hours in Ar ambient at 1 1 3 0 ∘ C and pulse laser annealing were applied to modify the structure of films. Surprisingly, after annealing with such high-thermal budget, according to the FTIR data, the nearly stoichiometric silicon nitride film contains hydrogen in the form of Si–H bonds. From analysis of the FTIR data of the Si–N bond vibrations, one can conclude that silicon nitride is partly crystallized. According to the Raman data a - S i : H films with hydrogen concentration 15% and lower contain mainly Si–H chemical species, and films with hydrogen concentration 30–35% contain mainly Si–H 2 chemical species. Nanosecond pulse laser treatments lead to crystallization of the films and its dehydrogenization.
不同化学计量的氢化富硅氮化物(S i N≥:H)的振动性质。6≤≤1。利用拉曼光谱和傅里叶变换红外光谱对氢化非晶硅(a - S i: H)薄膜进行了研究。采用11330°C氩气环境下5小时的炉内退火和脉冲激光退火来改变薄膜的结构。令人惊讶的是，经过如此高的热收支退火后，根据FTIR数据，几乎化学计量的氮化硅薄膜含有Si-H键形式的氢。通过分析Si-N键振动的FTIR数据，可以得出氮化硅部分结晶的结论。根据拉曼数据，氢浓度为15%及以下的a - Si: H薄膜主要含有Si-H化学物质，氢浓度为30-35%的薄膜主要含有si - h2化学物质。纳秒脉冲激光处理导致薄膜的结晶和脱氢。
{"title":"Vibrational Spectroscopy of Chemical Species in Silicon and Silicon-Rich Nitride Thin Films","authors":"K. O. Bugaev, A. Zelenina, V. Volodin","doi":"10.1155/2012/281851","DOIUrl":"https://doi.org/10.1155/2012/281851","url":null,"abstract":"Vibrational properties of hydrogenated silicon-rich nitride ( S i N 𝑥 : H ) of various stoichiometry ( 0 . 6 ≤ 𝑥 ≤ 1 . 3 ) and hydrogenated amorphous silicon ( a - S i : H ) films were studied using Raman spectroscopy and Fourier transform infrared spectroscopy. Furnace annealing during 5 hours in Ar ambient at 1 1 3 0 ∘ C and pulse laser annealing were applied to modify the structure of films. Surprisingly, after annealing with such high-thermal budget, according to the FTIR data, the nearly stoichiometric silicon nitride film contains hydrogen in the form of Si–H bonds. From analysis of the FTIR data of the Si–N bond vibrations, one can conclude that silicon nitride is partly crystallized. According to the Raman data a - S i : H films with hydrogen concentration 15% and lower contain mainly Si–H chemical species, and films with hydrogen concentration 30–35% contain mainly Si–H 2 chemical species. Nanosecond pulse laser treatments lead to crystallization of the films and its dehydrogenization.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"2 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2012-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83530769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ZnO nanorods prepared through chemical vapor deposition technique are characterized by microscopic and X-ray photoelectron spectroscopy (XPS) techniques to correlate the effects of size on the binding energy of Zn 2p3/2 photoelectrons. A positive shift in Zn 2p3/2-binding energy as compared to that in bulk ZnO is assumed to be the effect of size of ZnO tips. The shift in binding energy has been explained in terms of relaxation energy in the photoemission process. Simultaneously, Auger parameter of the nanorods is evaluated for stoichiometric composition. The extra peak in O1s spectrum of nanorods is explained as adsorbed O-bearing species or surface contaminants.
{"title":"Analysis on Binding Energy and Auger Parameter for Estimating Size and Stoichiometry of ZnO Nanorods","authors":"S. Bera, S. Dhara, S. Velmurugan, A. K. Tyagi","doi":"10.1155/2012/371092","DOIUrl":"https://doi.org/10.1155/2012/371092","url":null,"abstract":"ZnO nanorods prepared through chemical vapor deposition technique are characterized by microscopic and X-ray photoelectron spectroscopy (XPS) techniques to correlate the effects of size on the binding energy of Zn 2p3/2 photoelectrons. A positive shift in Zn 2p3/2-binding energy as compared to that in bulk ZnO is assumed to be the effect of size of ZnO tips. The shift in binding energy has been explained in terms of relaxation energy in the photoemission process. Simultaneously, Auger parameter of the nanorods is evaluated for stoichiometric composition. The extra peak in O1s spectrum of nanorods is explained as adsorbed O-bearing species or surface contaminants.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"20 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2012-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90869075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Atanu Sengupta, C. Shende, S. Farquharson, Frank Inscore
The need for portable technologies that can rapidly identify biological warfare agents (BWAs) in the field remains an international priority as expressed at the 2011 Biological Weapons Convention. In recent years, the ability of surface-enhanced Raman spectroscopy (SERS) to rapidly detect various BWAs at very low concentrations has been demonstrated. However, in the specific case of Bacillus anthracis, differentiation at the species level is required since other bacilli are common in the environment, representing potential false-positive responses. To overcome this limitation, we describe the use of a peptide attached to the SERS-active metal that selectively binds Bacillus anthracis-Sterne as the target analyte. Using this approach, 109 B. anthracis-Sterne spores/mL produced an intense dipicolinic acid spectrum upon the addition of acetic acid, while the same concentration and treatment of B. cereus and B. subtilis did not.
{"title":"Detection of Bacillus anthracis Spores Using Peptide Functionalized SERS-Active Substrates","authors":"Atanu Sengupta, C. Shende, S. Farquharson, Frank Inscore","doi":"10.1155/2012/176851","DOIUrl":"https://doi.org/10.1155/2012/176851","url":null,"abstract":"The need for portable technologies that can rapidly identify biological warfare agents (BWAs) in the field remains an international priority as expressed at the 2011 Biological Weapons Convention. In recent years, the ability of surface-enhanced Raman spectroscopy (SERS) to rapidly detect various BWAs at very low concentrations has been demonstrated. However, in the specific case of Bacillus anthracis, differentiation at the species level is required since other bacilli are common in the environment, representing potential false-positive responses. To overcome this limitation, we describe the use of a peptide attached to the SERS-active metal that selectively binds Bacillus anthracis-Sterne as the target analyte. Using this approach, 109 B. anthracis-Sterne spores/mL produced an intense dipicolinic acid spectrum upon the addition of acetic acid, while the same concentration and treatment of B. cereus and B. subtilis did not.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"44 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2012-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82217969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：