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Inhibition of metastasis using particles that release chitosan upon radiation: A preliminary study 放射释放壳聚糖颗粒抑制肿瘤转移的初步研究
Pub Date : 2019-01-01 DOI: 10.1142/S0129083519500104
S. Harada, Takahiro Sato
This study aimed to investigate the effect of the particles releasing chitosan upon exposure to radiation on inhibition of metastasis. A 10 mL solution of water containing 0.2% weight/volume alginate, 0.1% hyaluronic acid, and 100-mg chitosan was sprayed into the vibrating solution through a stainless mesh filter (pore size: 0.8 [Formula: see text]m) using an ultrasound disintegrator, thereby generating chitosan particles. Further, [Formula: see text] particles floating in 0.1 mL normal saline were subcutaneously injected around the 4TI cells-derived tumor in the left hind legs of six-week-old male C3He/N mice. Six hours after injection, tumors were exposed to 10 Gy or 20 Gy of 100-keV soft X-ray radiation. The release of chitosan was expressed as the frequency of ruptured chitosan particles 12 h after radiation. The antimetastatic effect was confirmed by a reduction in the number of metastatic pulmonary nodules 21 days after completion of treatment. More than [Formula: see text]% of the chitosan particles released chitosan in response to radiation. The particles releasing chitosan had a prolonged antimetastatic effect when compared with the particles not releasing chitosan, thereby resulting in a significantly greater antimetastatic effect lasting for four weeks since the completion of treatment, in tumors treated with both 10 Gy and 20 Gy of radiation. Hence, particlizing chitosan could be useful in reducing metastasis in irradiated tumors.
本研究旨在探讨受辐射后释放壳聚糖对肿瘤转移的抑制作用。将含有0.2%重量/体积海藻酸盐、0.1%透明质酸和100 mg壳聚糖的10 mL水溶液,通过超声波破碎机将孔径为0.8 m的不锈钢网过滤器(公式:见文)喷入振动溶液中,生成壳聚糖颗粒。在6周龄雄性C3He/N小鼠左后腿4TI细胞源性肿瘤周围皮下注射悬浮于0.1 mL生理盐水中的颗粒。注射后6小时,肿瘤分别接受10 Gy或20 Gy的100 kev软x射线照射。壳聚糖的释放量表示为辐照后12 h壳聚糖颗粒破裂的频率。治疗结束后21天,转移性肺结节数量减少,证实了抗转移效果。超过[公式:见文]%的壳聚糖颗粒释放壳聚糖响应辐射。与不释放壳聚糖的颗粒相比,释放壳聚糖的颗粒具有较长的抗转移作用,因此在接受10 Gy和20 Gy放射治疗的肿瘤中,在治疗结束后的四周内,其抗转移作用明显更大。因此,壳聚糖颗粒化可能有助于减少放射肿瘤的转移。
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引用次数: 0
Quantification of major and trace elements contained in aircraft JET A-1 fuel by in-vacuum PIXE analysis JET A-1飞机燃料中主要和微量元素的定量分析
Pub Date : 2019-01-01 DOI: 10.1142/S0129083519500165
K. Saitoh, A. Fushimi, N. Takegawa, K. Sera
To characterize the chemical composition of aircraft exhaust particles, we developed a treatment method of jet fuel for an elemental analysis by an in-vacuum PIXE system. Eleven elements (Si, S, Cl, K, Ca, Cr, Fe, Ni, Cu, Zn, and Pb) were identified from each sample. The concentrations of S from five JET A-1 fuel samples collected on different days ranged from 30.4 to 440 wt.-ppm. The concentration level of S agreed well with the measurement results obtained by an in-air PIXE analysis, which we have previously performed to determine the major content elements and their concentration levels. Nine elements out of the identified 11 elements (Si, Cl, K, Ca, Cr, Ni, Cu, Zn, and Pb), which were not detected by the in-air PIXE analysis, were detected in all the JET A-1 fuel samples measured. Among these elements, Si, Ni, Cu, Zn, and Pb were found to be the major components. It is suggested that particles emitted from aircraft jet engines, which are generally in the size range smaller than 100 nm (ultrafine particles: UFPs), may contain Si, Ni, Cu, Zn, and Pb. These findings provide useful insights into the source apportionment of UFPs in and around airports.
为了表征飞机废气颗粒的化学成分,我们开发了一种用于真空pxie系统元素分析的航空燃料处理方法。从每个样品中鉴定出11种元素(Si, S, Cl, K, Ca, Cr, Fe, Ni, Cu, Zn和Pb)。5个JET A-1燃料样品在不同日期的S浓度范围为30.4 ~ 440 wt.-ppm。S的浓度水平与我们之前进行的测定主要含量元素及其浓度水平的空气中PIXE分析的测量结果吻合得很好。所鉴定的11种元素(Si、Cl、K、Ca、Cr、Ni、Cu、Zn和Pb)中,有9种元素在JET A-1燃料样品中均被检测到,而这些元素是空气中PIXE分析无法检测到的。其中,Si、Ni、Cu、Zn和Pb是主要成分。飞机喷气发动机排放的颗粒,通常在小于100 nm的尺寸范围内(超细颗粒:UFPs),可能含有Si, Ni, Cu, Zn和Pb。这些发现为机场内外ufp的来源分配提供了有用的见解。
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引用次数: 1
Long-term and season-specific changes in the body concentrations of iron, copper, zinc, and selenium by means of quantitative analyses of beard samples collected every day — A case study 通过对每天收集的胡须样本进行定量分析,研究人体铁、铜、锌和硒浓度的长期和季节变化-一个案例研究
Pub Date : 2019-01-01 DOI: 10.1142/s0129083519500207
K. Sera, S. Goto, T. Hosokawa, Y. Saitoh
A total of 4269 beard samples were collected from the same person every day over a 12-year period and analyzed by PIXE using a standard-free method. It was found that the concentrations of copper and zinc showed certain short-term changes but did not show a noticeable long-term trend over the study period, with only iron showing a slightly decreasing tendency with age. All of these elements showed clear yearly variations with a cycle of a few years possibly due to periodic metabolic changes in the subject’s body or long-term changes in eating habits. In contrast, however, selenium showed clear seasonal variations. Its concentration significantly increased in the summer and decreased in the winter, just as was observed with arsenic and mercury. This suggests that most of the subject’s selenium intake was from marine products, whose supply and consumption were increased in the summer. These findings confirmed that beard analyses are useful not only for evaluating essential-element intake but also for estimating the relationship between the body-element concentrations and ingestion of certain foods.
在12年的时间里,每天从同一个人身上收集4269份胡须样本,并通过PIXE使用无标准方法进行分析。研究发现,铜和锌的浓度在研究期间有一定的短期变化,但没有明显的长期趋势,只有铁随年龄的增长略有下降。所有这些因素都显示出明显的年变化,周期为几年,可能是由于受试者体内的周期性代谢变化或饮食习惯的长期变化。相反,硒表现出明显的季节变化。其浓度在夏季显著升高,在冬季显著降低,正如砷和汞所观察到的那样。这表明受试者的硒摄入量主要来自海产品,其供应和消费量在夏季增加。这些发现证实,胡须分析不仅对评估必需元素的摄入量有用,而且对估计人体元素浓度与某些食物摄入之间的关系也有用。
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引用次数: 0
Analysis of tooth-bound fluoride after the application of coating materials on the enamel surface with an in-air micro PIXE/PIGE 在牙釉质表面应用涂层材料后,用空气微型PIXE/PIGE分析牙结合氟化物
Pub Date : 2019-01-01 DOI: 10.1142/s0129083519500116
K. Okuyama, Y. Tamaki, Y. Matsuda, T. Saito, H. Yamamoto, K. Naito, M. Hayashi, K. Suzuki
Tooth-bound (KOH-insoluble) fluoride prevents caries via long retention within the tooth structure. Tooth-bound fluoride in the enamel was investigated after applying various fluoride-containing tooth-coating materials using an in-air micro [Formula: see text] PIXE/PIGE system. Three different fluoride-containing or a non-fluoride containing coating materials were applied to the coronal enamel surface of 24 extracted human molars. The samples were stored in water for 1 or 7 days and then sectioned into two slices. One slice was immersed in 1M KOH solution for 24 h for KOH-insoluble fluoride (KOH(T)) analysis. The second slice was not subjected to KOH treatment and was used to analyze the whole fluoride (KOH-soluble plus insoluble fluoride: KOH(U)). Fluorine and calcium distribution were measured in all the slices with [Formula: see text]PIXE/PIGE. KOH(U) was higher than KOH(T) on some materials, especially those that released more fluoride. For KOH(T), there was no significant difference among all fluoride-containing materials in the 7-day group. However, there were significant differences in the KOH(U) samples during the same period. PIXE/PIGE analysis confirmed the presence of tooth-bound fluoride in the enamel adjacent to the fluoride-containing coating materials. The amount of tooth-bound fluoride depended on the type of material.
结合在牙齿上的氟化物(氢氧化钾不溶性)通过在牙齿结构中长时间保留来防止龋齿。在使用空气微型[配方:见文本]PIXE/PIGE系统涂抹各种含氟牙齿涂层材料后,研究了牙釉质中结合的氟化物。采用三种不同的含氟或不含氟涂层材料对24颗拔除的人磨牙的冠状牙釉质表面进行涂层处理。样品在水中保存1天或7天,然后切成两片。其中一片在1M KOH溶液中浸泡24 h,进行KOH不溶性氟化物(KOH(T))分析。第二层未进行KOH处理,用于分析全氟(KOH-可溶性加不溶性氟:KOH(U))。用[公式:见文]PIXE/PIGE测定各切片中氟、钙的分布。某些材料的KOH(U)高于KOH(T),特别是那些释放较多氟化物的材料。对于KOH(T), 7 d组各含氟材料间无显著差异。然而,同一时期的KOH(U)样品存在显著差异。PIXE/PIGE分析证实,在含氟涂层材料附近的牙釉质中存在与牙齿结合的氟化物。牙齿上氟化物的含量取决于材料的种类。
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引用次数: 1
Development of a 20MeV proton particle-induced X-ray emission analysis method in a helium atmosphere 氦大气中20MeV质子粒子诱导x射线发射分析方法的建立
Pub Date : 2019-01-01 DOI: 10.1142/s0129083519500219
K. Ishii, A. Terakawa, K. Hitomi, M. Nogami
We developed a 20[Formula: see text]MeV particle-induced X-ray emission (PIXE) analysis method using a medical cyclotron, which is conventionally used for positron emission tomography analysis performed in vacuo, during which the target sample is damaged. For non-destructive analysis and ease of switching between target samples, we developed a technique allowing 20[Formula: see text]MeV proton PIXE analysis to be performed at the atmospheric pressure. We filled the PIXE analysis chamber with helium and checked that the continuous background of the Compton tails of nuclear reaction [Formula: see text]-rays increased only minimally, and that the quasi-free electron bremsstrahlung (QFEB) did not increase at all, in the X-ray energy spectrum.
我们利用医用回旋加速器开发了一种20 MeV粒子诱导x射线发射(PIXE)分析方法,该方法通常用于在真空中进行的正电子发射断层扫描分析,在此过程中目标样品被破坏。为了非破坏性分析和易于在目标样品之间切换,我们开发了一种技术,允许在大气压下进行20[公式:见文本]MeV质子PIXE分析。我们用氦填充了PIXE分析室,并检查了核反应康普顿尾射线的连续背景仅轻微增加,并且在x射线能谱中准自由电子轫致辐射(QFEB)根本没有增加。
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引用次数: 0
FT-IR measurement on the damage of Japanese paper induced by PIXE analysis FT-IR对日本纸pixie损伤的测量
Pub Date : 2019-01-01 DOI: 10.1142/S012908351950013X
Y. Oguri, J. Hasegawa, H. Fukuda, N. Hagura
Analysis of cultural heritage samples by PIXE involves the risk of damaging precious samples due to MeV-proton irradiation. To investigate appropriate methods to detect invisible damage due to PIXE analysis, we analyzed the change in chemical bonding of the sample surface subjected to PIXE and RBS measurement by Fourier Transform InfraRed spectroscopy (FT-IR). We used Japanese hemp paper as a simulated cultural property sample. The proton irradiation for the PIXE/RBS measurement was performed in a vacuum with an incident beam energy of 2.5 MeV, a beam current of 1 nA, and an irradiation time up to 10 min. The corresponding beam flux and fluence were 0.06 nA/mm2 and 4 [Formula: see text]Coulomb/cm2, respectively. When the irradiation time was 10 min, the absorbed dose was 480 kGy on the sample surface. We identified neither change of elemental composition nor visible change such as discoloration due to irradiation. However, we found changes in peak heights in the measured FT-IR spectra, which suggest the destruction of chemical bonds such as O–H and C–O due to proton-induced radiation damage.
PIXE对文化遗产样本的分析涉及到由于mev质子辐照而损坏珍贵样本的风险。为了寻找合适的方法来检测由于PIXE分析造成的不可见损伤,我们利用傅里叶变换红外光谱(FT-IR)分析了样品表面经过PIXE和RBS测量的化学键的变化。我们使用日本麻纸作为模拟文物样本。用于PIXE/RBS测量的质子辐照是在真空中进行的,入射光束能量为2.5 MeV,光束电流为1 nA,辐照时间为10 min,相应的束流和通量分别为0.06 nA/mm2和4[公式:见文本]库仑/cm2。辐照时间为10 min时,样品表面的吸收剂量为480 kGy。我们既没有发现元素组成的变化,也没有发现辐照引起的变色等可见变化。然而,我们在测量的FT-IR光谱中发现了峰高的变化,这表明由于质子诱导的辐射损伤,O-H和C-O等化学键被破坏。
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引用次数: 0
Adsorption behavior of Cs+ ions to vermiculite demonstrated by molecular dynamics simulations 分子动力学模拟研究了Cs+离子在蛭石上的吸附行为
Pub Date : 2018-01-01 DOI: 10.1142/s0129083519500013
A. Takeuchi
The present paper discusses the simulation results, performed by classical molecular dynamics (MD), for vermiculite. The kinetic process of the Cs+ ions in water that are adsorbed into vermiculite ...
本文讨论了经典分子动力学(MD)对蛭石的模拟结果。研究了水中Cs+离子吸附到蛭石上的动力学过程。
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引用次数: 1
Mineral pigment studies on ancient ceramics and glass artifacts from commercial settlements on Danube border between Muntenia and Dobruja 蒙泰尼亚和多布鲁加之间多瑙河边界商业定居点古代陶瓷和玻璃文物的矿物颜料研究
Pub Date : 2018-01-01 DOI: 10.1142/s0129083519500025
B. Constantinescu, D. Cristea-Stan, I. Kovács, Z. Szökefalvi-nagy, I. Poll
We analyzed the compositions of mineral pigments for some ceramics and glass items excavated from the commercial settlements on Danube at the border between Ottoman Empire (Dobruja) and Romanian Pr...
我们分析了在奥斯曼帝国(多布鲁加)和罗马尼亚共和国边界多瑙河上的商业定居点出土的一些陶瓷和玻璃制品的矿物颜料组成。
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引用次数: 0
Analysis of strontium in soil and plant samples contaminated during the 2011 Fukushima Daiichi Nuclear Power Plant accident 2011年福岛第一核电站事故污染土壤和植物样品中的锶分析
Pub Date : 2018-01-01 DOI: 10.1142/s0129083519500049
K. Ishii, H. Arai, Tohru Ohnuma, A. Takeuchi
An accident in March 2011 released small amounts of radioactive strontium isotopes (90Sr) along with large amounts of radioactive caesium isotopes (137Cs). 90Sr isotopes accumulate in bone and are ...
2011年3月的一次事故释放了少量放射性锶同位素(90Sr)和大量放射性铯同位素(137Cs)。90Sr同位素在骨骼中积累,并且…
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引用次数: 1
Elemental characterization of gold-plated costume jewelry using particle-induced X-ray emission 使用粒子诱导x射线发射的镀金人造珠宝的元素表征
Pub Date : 2018-01-01 DOI: 10.1142/s0129083519500074
R. Debastiani, I. Almansa, L. A. Boufleur, T. M. Ferrari, C. T. Souza, D. Bauer, L. Amaral, J. Dias
Nine gold-plated costume jewelries bought in the street market of Porto Alegre and from Chinese websites were investigated through Particle-Induced X-ray Emission (PIXE) and Rutherford Backscatteri...
通过粒子诱导x射线发射(PIXE)和卢瑟福反向散射(Rutherford backscatter)对在阿雷格里港街头市场和中国网站上购买的9件镀金人造珠宝进行了研究。
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引用次数: 1
期刊
International Journal of PIXE
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