Pub Date : 2023-06-16DOI: 10.52419/issn2072-2419.2023.1.64
R. Slobodyanik, S. Zykova, A. Lunegov, O. Shcherbakov, E. Engasheva
Due to the ability of ixodid ticks to transmit pathogens transovarially and transphasically, natural foci of dangerous diseases are formed, which is important for preventive measures. Of particular danger are foci of diseases that have formed on the territory of settlements. The aim of our study was to determine the therapeutic efficacy of the insectoacaricidal drug for veterinary use "OKVET Tabs Express for dogs". In the period from June 24 to August 27, 2022, the study drug was tested on 28 dogs of both sexes with a confirmed diagnosis of ixodidosis aged from 10 months to 10 years in the farms of the Meghri district of the Syunik region of the Republic of Armenia. The species affiliation of ticks found in the studied dogs was determined jointly with the specialists of the Laboratory of Molecular Parasitology of the Scientific Center of Zoology and Hydroecology of the National Academy of Sciences of the Republic of Armenia using determinants. According to the results of the experiment, it was found that 40% of all ixodid ticks found on dogs were R. turanicus Pomerantsev 1936, and ticks R. sanguineus Latreille, 1806 and H. scupense Schulze, 1919 were found in 30% of cases. After a single oral administration of the drug, we did not observe the presence of ticks in animals during daily examination for 65 days. In animals during the entire experiment, no deviations from physiological norms and anxiety were found, signs of allergic and other visible side effects were not detected
由于粘蜱具有经卵巢和经卵巢传播病原体的能力，形成了危险疾病的自然疫源地，对预防具有重要意义。特别危险的是在定居点领土上形成的疾病焦点。本研究的目的是确定兽用杀虫螨药“OKVET狗片特快”的治疗效果。在2022年6月24日至8月27日期间，研究人员在亚美尼亚共和国Syunik地区Meghri区的农场对28只雌雄犬进行了研究药物测试，这些犬的年龄在10个月至10岁之间，确诊为顽固性疾病。与亚美尼亚共和国国家科学院动物和水文生态学科学中心分子寄生虫学实验室的专家共同确定了在所研究的狗身上发现的蜱虫的物种归属。根据实验结果，在犬身上发现的所有伊蚊蜱中，40%为turanicus R. Pomerantsev 1936, 30%为血蜱R. Latreille, 1806和H. scupense Schulze, 1919。在单次口服该药物后，我们在65天的日常检查中未观察到动物中蜱虫的存在。在整个实验过程中，没有发现动物偏离生理规范和焦虑，没有发现过敏迹象和其他明显的副作用
{"title":"Study of the therapeutic efficacy of okvet","authors":"R. Slobodyanik, S. Zykova, A. Lunegov, O. Shcherbakov, E. Engasheva","doi":"10.52419/issn2072-2419.2023.1.64","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.1.64","url":null,"abstract":"Due to the ability of ixodid ticks to transmit pathogens transovarially and transphasically, natural foci of dangerous diseases are formed, which is important for preventive measures. Of particular danger are foci of diseases that have formed on the territory of settlements. The aim of our study was to determine the therapeutic efficacy of the insectoacaricidal drug for veterinary use \"OKVET Tabs Express for dogs\". In the period from June 24 to August 27, 2022, the study drug was tested on 28 dogs of both sexes with a confirmed diagnosis of ixodidosis aged from 10 months to 10 years in the farms of the Meghri district of the Syunik region of the Republic of Armenia. The species affiliation of ticks found in the studied dogs was determined jointly with the specialists of the Laboratory of Molecular Parasitology of the Scientific Center of Zoology and Hydroecology of the National Academy of Sciences of the Republic of Armenia using determinants. According to the results of the experiment, it was found that 40% of all ixodid ticks found on dogs were R. turanicus Pomerantsev 1936, and ticks R. sanguineus Latreille, 1806 and H. scupense Schulze, 1919 were found in 30% of cases. After a single oral administration of the drug, we did not observe the presence of ticks in animals during daily examination for 65 days. In animals during the entire experiment, no deviations from physiological norms and anxiety were found, signs of allergic and other visible side effects were not detected","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86851249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-16DOI: 10.52419/issn2072-2419.2023.1.98
O. Evdokimova, S. Engashev, A. Novak, M. Novak, E. Engasheva
Tests of drugs Florfenicol 40% LLC «AVZ SP » and Florox LLC «NITA PHARM» from the group of fenicols for bacterial respiratory and intestinal infections were carried out on 43 calves 1.5- 3.5 months old in a large dairy complex in the Central District of the Russian Federation.Based on the results of bacteriological studies before the use of antibiotics Florfenicol 40% and Florox, in lavages from the upper respiratory tract and intestines of calves of experimental and control groups, Corynebacterium spp., Staphylococcus spp., Staphylococcus aureus, Streptococcus spp., Enterococcus spp., Micrococcus spp., Moraxella bovis, Pseudomonas spp., Proteus spp., Bordetella bronchiseptica, Neisseria spp., Clostridium spp., Escherichia spp., Enterobacter spp., Citrobacter spp., Serratia spp., Edvardsiella spp., Providencia spp., Salmonella spp., including pathogenic serotypes and bacterial strains.The results of bacteriological studies of isolated cultures from calves of experimental and control groups after specific therapy showed the absence of pathogenic serotypes and strains of Escherichia coli, Staphylococcus aureus, Bordetella bronchiseptica, Pseudomonas spp., Salmonella spp. in swabs from the nasal cavity and rectum, but the presence of non-fermentative forms of Escherichia spp. and other opportunistic bacteria.Based on the results obtained, it was found that the drug Florfenicol 40% in doses of 1 ml per 20 kg of body weight intramuscularly twice with an interval of 48 hours and 2 ml per 20 kg subcutaneously once has a pronounced activity against aerobic and facultative anaerobic bacteria. According to the results of hematological and biochemical studies, the antibiotic Florfenicol 40% does not have a negative effect on homeostasis with a positive effect on metabolic processes and a decrease in the activity of acute and chronic inflammation in tissues and organs
{"title":"Study of the therapeutic efficacy of the drug florfenicolin respirato-ry and intestinalbacterial infections of cattle","authors":"O. Evdokimova, S. Engashev, A. Novak, M. Novak, E. Engasheva","doi":"10.52419/issn2072-2419.2023.1.98","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.1.98","url":null,"abstract":"Tests of drugs Florfenicol 40% LLC «AVZ SP » and Florox LLC «NITA PHARM» from the group of fenicols for bacterial respiratory and intestinal infections were carried out on 43 calves 1.5- 3.5 months old in a large dairy complex in the Central District of the Russian Federation.Based on the results of bacteriological studies before the use of antibiotics Florfenicol 40% and Florox, in lavages from the upper respiratory tract and intestines of calves of experimental and control groups, Corynebacterium spp., Staphylococcus spp., Staphylococcus aureus, Streptococcus spp., Enterococcus spp., Micrococcus spp., Moraxella bovis, Pseudomonas spp., Proteus spp., Bordetella bronchiseptica, Neisseria spp., Clostridium spp., Escherichia spp., Enterobacter spp., Citrobacter spp., Serratia spp., Edvardsiella spp., Providencia spp., Salmonella spp., including pathogenic serotypes and bacterial strains.The results of bacteriological studies of isolated cultures from calves of experimental and control groups after specific therapy showed the absence of pathogenic serotypes and strains of Escherichia coli, Staphylococcus aureus, Bordetella bronchiseptica, Pseudomonas spp., Salmonella spp. in swabs from the nasal cavity and rectum, but the presence of non-fermentative forms of Escherichia spp. and other opportunistic bacteria.Based on the results obtained, it was found that the drug Florfenicol 40% in doses of 1 ml per 20 kg of body weight intramuscularly twice with an interval of 48 hours and 2 ml per 20 kg subcutaneously once has a pronounced activity against aerobic and facultative anaerobic bacteria. According to the results of hematological and biochemical studies, the antibiotic Florfenicol 40% does not have a negative effect on homeostasis with a positive effect on metabolic processes and a decrease in the activity of acute and chronic inflammation in tissues and organs","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90907290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.52419/issn2072-2419.2023.1.11
E. Sizova, E. Yausheva, K. S. Nechitailo
Modern methods of nanotechnology make it possible to transform macro- and micro-objects into ultrafine particles (UFP), while the process itself is accompanied by a change in the chemical and physical properties of the base material. Naturally, the biological activity of complexes of UHF microelements will differ from that of individual UHFs. In this regard, the purpose of our study was to study the dependence of the biological activity of the UHF copper + zinc complex on their physicochemical characteristics.Analysis of the data obtained allowed us to state that sample «C» had a high level of toxicity of the studied samples of UHF against the strain E. coli E. coli K12 TG1 pF1. Further, according to the observed effect, sample A was noted, while the effect of exposure to the studied UHF was dose- and time-dependent.As a result, the data obtained on the basis of the reaction of strains E. coli pSoxS::lux and E. coli pKatG-lux, the induction of luminescence of which is associated with the development of oxidative stress in cells, on the action of the studied samples of the copper+ zinc complex, made it possible to form a sample with the maximum effect «C». Further, according to the degree of development of the toxic effect, samples «B» and «A» decreased in the series. At the same time, the interpretation of the results of the response of the E. coli pRecA -lux strain, the induction of which is caused by DNA damage in cells, forms a different series of specificity: the greatest effect on bacterial cells among the studied substances, sample B was rendered, while the maximum luminescence amplitude was 70 units at 0.002 M, and the sensitivity threshold was at a concentration of 0.0004 M.Based on the data obtained, in our study, the degree of biological activity of UHF for a living system was determined by a combination of a number of physicochemical parameters: shape, phase composition, and specific surface area
{"title":"Comparative evaluation of the biological activity of a complex of ultrafine particles based on copper and zinc in the bioluminescence inhibition test","authors":"E. Sizova, E. Yausheva, K. S. Nechitailo","doi":"10.52419/issn2072-2419.2023.1.11","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.1.11","url":null,"abstract":"Modern methods of nanotechnology make it possible to transform macro- and micro-objects into ultrafine particles (UFP), while the process itself is accompanied by a change in the chemical and physical properties of the base material. Naturally, the biological activity of complexes of UHF microelements will differ from that of individual UHFs. In this regard, the purpose of our study was to study the dependence of the biological activity of the UHF copper + zinc complex on their physicochemical characteristics.Analysis of the data obtained allowed us to state that sample «C» had a high level of toxicity of the studied samples of UHF against the strain E. coli E. coli K12 TG1 pF1. Further, according to the observed effect, sample A was noted, while the effect of exposure to the studied UHF was dose- and time-dependent.As a result, the data obtained on the basis of the reaction of strains E. coli pSoxS::lux and E. coli pKatG-lux, the induction of luminescence of which is associated with the development of oxidative stress in cells, on the action of the studied samples of the copper+ zinc complex, made it possible to form a sample with the maximum effect «C». Further, according to the degree of development of the toxic effect, samples «B» and «A» decreased in the series. At the same time, the interpretation of the results of the response of the E. coli pRecA -lux strain, the induction of which is caused by DNA damage in cells, forms a different series of specificity: the greatest effect on bacterial cells among the studied substances, sample B was rendered, while the maximum luminescence amplitude was 70 units at 0.002 M, and the sensitivity threshold was at a concentration of 0.0004 M.Based on the data obtained, in our study, the degree of biological activity of UHF for a living system was determined by a combination of a number of physicochemical parameters: shape, phase composition, and specific surface area","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86903539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-15DOI: 10.52419/issn2072-2419.2023.1.20
S. Makavchik, A. Krotova
For causative agents of purulent-septic processes in animals, the following features are characteristic: polytropism, polyetiology, polyhostality, plasticity and polyadaptationThe purpose of the study was to rank clinically significant pathogens of purulentseptic processes in animals in veterinary practice.Methods. Based on the results for the period 2021-2022, we conducted bacteriological studies of 87 biomaterial samples from sick dogs and cats with skin lesions (abscesses, wounds), otitis media, cystitis, rhinitis, as well as from the corpses of puppies at the age of 3 days.Identification of streptococci and enterococci to species was carried out using the test system: STREPTOtest 24 (Erba Lachema, Czech Republic) and api 20 Strep (BIOMERIEUX, France), api 20 E and Rapid 20 E (BIOMERIEUX) were used to identify enterobacteria. ”, France), Nefermtest 24 (“Erba Lachema”, Czech Republic) was used to identify gram-negative non-fermenting bacteria.Results. 100 isolates were isolated from dogs with purulent-inflammatory processes, among which 18% are gram-negative and 82% are gram-positive microorganisms.
{"title":"Etiological structure of purulent-septic infections of animals in veterinary practice","authors":"S. Makavchik, A. Krotova","doi":"10.52419/issn2072-2419.2023.1.20","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2023.1.20","url":null,"abstract":"For causative agents of purulent-septic processes in animals, the following features are characteristic: polytropism, polyetiology, polyhostality, plasticity and polyadaptationThe purpose of the study was to rank clinically significant pathogens of purulentseptic processes in animals in veterinary practice.Methods. Based on the results for the period 2021-2022, we conducted bacteriological studies of 87 biomaterial samples from sick dogs and cats with skin lesions (abscesses, wounds), otitis media, cystitis, rhinitis, as well as from the corpses of puppies at the age of 3 days.Identification of streptococci and enterococci to species was carried out using the test system: STREPTOtest 24 (Erba Lachema, Czech Republic) and api 20 Strep (BIOMERIEUX, France), api 20 E and Rapid 20 E (BIOMERIEUX) were used to identify enterobacteria. ”, France), Nefermtest 24 (“Erba Lachema”, Czech Republic) was used to identify gram-negative non-fermenting bacteria.Results. 100 isolates were isolated from dogs with purulent-inflammatory processes, among which 18% are gram-negative and 82% are gram-positive microorganisms.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88505604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-04DOI: 10.52419/issn2072-2419.2022.4.445
E. L. Bezruk, T. Romanova
The article discusses the issues of changes in the state of the musculoskeletal system, in particular the skeletal system, in ewes and lambs with thyroid pathologies with iodine deficiency, kept in the conditions of peasant farms in the Beisky district of the Republic of Khakassia. The results are obtained from 20 ewes and their lambs with severe thyroid hyperplasia, for the period from 2020 to 2021. In the study of sheep using clinical, biochemical and radiological methods of research, they turned to the clinical status of animals with details on the musculoskeletal system: the state of muscles, joints, bones. As a result of the research, it was found that system-wide deviations in the body of sheep with endemic goiter and hypothyroidism are caused by the complex effect of iodine deficiency, against the background of an increased content of fluorine, cobalt, calcium in animal feed and water, which is due to being in an ecologically unfavorable zone. Endemic goiter and hypothyroidism occurs in adult sheep in a latent form and is clinically expressed in their offspring. Clinical signs of hypothyroidism in lambs are expressed by the development of myxedema, the development of myopathies and osteoarthropathy, caused by disturbances in oxidative processes and thermogenesis in the body. X-ray signs of thyroid osteoarthropathies are expressed by a slowdown in osteogenesis, especially epiphyseal growth zones of the type of punctate dysplasia, more pronounced on the pelvic limbs, the presence of moderate osteoporosis with an elevated level of calcium in the blood. The established biochemical abnormalities in blood parameters are characterized by an increase in the level of liver enzymes: ALT, AST, alkaline phosphatase and are directly proportional to the severity of clinical signs and the degree of thyroid hyperplasia.
{"title":"Osteoartropathies associated with hypoteriosis in endemic goiter in sheep in the Republic of Khakasia","authors":"E. L. Bezruk, T. Romanova","doi":"10.52419/issn2072-2419.2022.4.445","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2022.4.445","url":null,"abstract":"The article discusses the issues of changes in the state of the musculoskeletal system, in particular the skeletal system, in ewes and lambs with thyroid pathologies with iodine deficiency, kept in the conditions of peasant farms in the Beisky district of the Republic of Khakassia. The results are obtained from 20 ewes and their lambs with severe thyroid hyperplasia, for the period from 2020 to 2021. In the study of sheep using clinical, biochemical and radiological methods of research, they turned to the clinical status of animals with details on the musculoskeletal system: the state of muscles, joints, bones. As a result of the research, it was found that system-wide deviations in the body of sheep with endemic goiter and hypothyroidism are caused by the complex effect of iodine deficiency, against the background of an increased content of fluorine, cobalt, calcium in animal feed and water, which is due to being in an ecologically unfavorable zone. Endemic goiter and hypothyroidism occurs in adult sheep in a latent form and is clinically expressed in their offspring. Clinical signs of hypothyroidism in lambs are expressed by the development of myxedema, the development of myopathies and osteoarthropathy, caused by disturbances in oxidative processes and thermogenesis in the body. X-ray signs of thyroid osteoarthropathies are expressed by a slowdown in osteogenesis, especially epiphyseal growth zones of the type of punctate dysplasia, more pronounced on the pelvic limbs, the presence of moderate osteoporosis with an elevated level of calcium in the blood. The established biochemical abnormalities in blood parameters are characterized by an increase in the level of liver enzymes: ALT, AST, alkaline phosphatase and are directly proportional to the severity of clinical signs and the degree of thyroid hyperplasia.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80003743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-04DOI: 10.52419/issn2072-2419.2022.4.434
V. V. Guliaeva, V. Gaponova
Entropion, or inversion of the edge of the eyelid, is a frequent multifactorial disease in which the ciliated edge of the eyelid is wrapped inside, which leads to permanent corneal trauma and eye irritation. Currently, subcutaneous fillers based on hyaluronic acid are used to correct entropion due to the fact that it does not have organ- and speciesspecificity and, therefore, carries a minimal risk of allergic reactions. The advantage of subcutaneous fillers based on HA is that the injection can be reversed with the development of complications with the help of drugs based on hyaluronidase. The assessment was carried out by the amount of periocular moistening, alopecia and/or hyperemia of the eyelids with manual rotation of the eyelids outward during the examination. All patients underwent a complete ophthalmological examination with a slit lamp and indirect ophthalmoscopy, a Schirmer test and intraocular pressure. The injection methods used included linear threading, a series of single-point injections, or a combination of them. The criteria for evaluating the effectiveness of the use of hyaluronic acid as a means for correcting entropion in animals were local reactions to skin filler, inflammatory processes in the eye area associated with a relapse of entropion, as well as the duration of the period before repeated administration of the drug. The technique of subcutaneous injection of the GC filler is a simple, reliable, effective, safe, prolonged procedure that requires a minimum amount of time, which allows you to immediately alleviate the patient's condition from mild to moderate severity as a result of entropion in cats and dogs. This procedure is especially preferable for age-related patients and patients with high anesthetic risk.
{"title":"Hyaluronic acid as a means to correct entropion in animals","authors":"V. V. Guliaeva, V. Gaponova","doi":"10.52419/issn2072-2419.2022.4.434","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2022.4.434","url":null,"abstract":"Entropion, or inversion of the edge of the eyelid, is a frequent multifactorial disease in which the ciliated edge of the eyelid is wrapped inside, which leads to permanent corneal trauma and eye irritation. Currently, subcutaneous fillers based on hyaluronic acid are used to correct entropion due to the fact that it does not have organ- and speciesspecificity and, therefore, carries a minimal risk of allergic reactions. The advantage of subcutaneous fillers based on HA is that the injection can be reversed with the development of complications with the help of drugs based on hyaluronidase. The assessment was carried out by the amount of periocular moistening, alopecia and/or hyperemia of the eyelids with manual rotation of the eyelids outward during the examination. All patients underwent a complete ophthalmological examination with a slit lamp and indirect ophthalmoscopy, a Schirmer test and intraocular pressure. The injection methods used included linear threading, a series of single-point injections, or a combination of them. The criteria for evaluating the effectiveness of the use of hyaluronic acid as a means for correcting entropion in animals were local reactions to skin filler, inflammatory processes in the eye area associated with a relapse of entropion, as well as the duration of the period before repeated administration of the drug. The technique of subcutaneous injection of the GC filler is a simple, reliable, effective, safe, prolonged procedure that requires a minimum amount of time, which allows you to immediately alleviate the patient's condition from mild to moderate severity as a result of entropion in cats and dogs. This procedure is especially preferable for age-related patients and patients with high anesthetic risk.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84799108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-04DOI: 10.52419/issn2072-2419.2022.4.440
V. A. Zolotukhina, E. L. Bezruk
The article evaluates the dynamics of toxemia and detoxification capabilities of various wound dressings in thermal burn injury in rats in the experiment. A wound dressing based on collagen and chitosan Collachit® and a membrane wound dressing containing a hyperosmolar solution in the cavity were compared. Studies of the dynamics of toxemia in burn disease after experimental thermal injury were carried out on Wistar rats (n=20). Analysis of the results of the study was carried out on the basis of clinical, morphological and biochemical parameters of the peripheral blood of rats with experimental burn injury. The study of the morphology of blood cells is carried out according to generally accepted methods. Biochemical manifestations of endotoxemia are established by the dynamics of ALT, AST, urea, creatinine. In the 1st group of rats (n=10), the Collachit® wound dressing was applied. In the 2nd group (n=10), a semi -permeable membrane filled with a hyperosmolar solution, constituting a necrolytic enzyme, was fixed on the surface of the burn. As a result, it was found that burn toxemia in rats proceeded in 2 phases and was accompanied by depression, food refusal, leukocytosis, increased LII, liver transferases, creatinine and urea. Hematological deviations were characterized by non-uniform dynamics, corresponding to the phase of burn toxemia with maximum increases on days 1-2 and 5-7, a fall on days 3-4, and days 8-10. Hematological manifestations of toxemia depended on the method of treatment and were less pronounced with the use of membrane wound dressings than with collachitis. External signs of toxemia do not in all cases correspond to the hematological and biochemical profile of toxemia, which indicates the possibility of a latent course of burn disease with some methods of treatment. The applied wound dressings had a pronounced antiseptic efficacy, characterized by the absence of purulent-inflammatory complications over the entire period of the experiment.
{"title":"Characteristics of endotoxemia in different ways of treatment of experimental burn in rats","authors":"V. A. Zolotukhina, E. L. Bezruk","doi":"10.52419/issn2072-2419.2022.4.440","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2022.4.440","url":null,"abstract":"The article evaluates the dynamics of toxemia and detoxification capabilities of various wound dressings in thermal burn injury in rats in the experiment. A wound dressing based on collagen and chitosan Collachit® and a membrane wound dressing containing a hyperosmolar solution in the cavity were compared. Studies of the dynamics of toxemia in burn disease after experimental thermal injury were carried out on Wistar rats (n=20). Analysis of the results of the study was carried out on the basis of clinical, morphological and biochemical parameters of the peripheral blood of rats with experimental burn injury. The study of the morphology of blood cells is carried out according to generally accepted methods. Biochemical manifestations of endotoxemia are established by the dynamics of ALT, AST, urea, creatinine. In the 1st group of rats (n=10), the Collachit® wound dressing was applied. In the 2nd group (n=10), a semi -permeable membrane filled with a hyperosmolar solution, constituting a necrolytic enzyme, was fixed on the surface of the burn. As a result, it was found that burn toxemia in rats proceeded in 2 phases and was accompanied by depression, food refusal, leukocytosis, increased LII, liver transferases, creatinine and urea. Hematological deviations were characterized by non-uniform dynamics, corresponding to the phase of burn toxemia with maximum increases on days 1-2 and 5-7, a fall on days 3-4, and days 8-10. Hematological manifestations of toxemia depended on the method of treatment and were less pronounced with the use of membrane wound dressings than with collachitis. External signs of toxemia do not in all cases correspond to the hematological and biochemical profile of toxemia, which indicates the possibility of a latent course of burn disease with some methods of treatment. The applied wound dressings had a pronounced antiseptic efficacy, characterized by the absence of purulent-inflammatory complications over the entire period of the experiment.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83166156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.52419/issn2072-2419.2022.4.401
V. I. Zimnikov, O. Manzhurina, E. V. Tyurina
The largest number of currently produced drugs for the treatment of mastitis in lactating cows contain antimicrobial components (antibiotics) as active substances. The studies were carried out on Red-Motley Holstein cows, in which subclinical mastitis was diagnosed. The animals selected for the experiment were divided into three groups. The first group included 8 animals, they were not treated and served as a negative control. In the second group, 10 cows were selected for treatment with Biferon-B for three days once a day at a dose of 10 ml. per animal. The animals of the third group were simultaneously administered interferon-λ and BiferonB intramuscularly, 10 ml each during those days. According to the results of clinical studies, it was found that no recovery was recorded in the animals in the negative control group. In the group of animals where Biferon-B was used, the efficacy of treatment was 60.0%, and with the combined use of Biferon-B and interferon-λ, the effect of treatment was 83.3%. In these animals, the number of somatic cells decreased by 14.3 times, the concentration of neutrophils – by 2.6 times, the amount of total Ig - by 67.5%, CIC - by 61.5%, against the background of an increase in the number of lymphocytes by 6.0 times, monocytes - by 2.1 times and lysozyme - by 45.3%, and the bacterial contamination of the udder secretion also decreased to 0.08 thousand CFU/ml, which was by 120 times less compared to the initial state. Only Escherichia coli has been isolated from the udder secretion in 20.0% of cases that confirms the clinical efficacy of the treatment.
{"title":"Indicators of mammary gland secretion in cows with subclinical mastitis when using interferon-λ","authors":"V. I. Zimnikov, O. Manzhurina, E. V. Tyurina","doi":"10.52419/issn2072-2419.2022.4.401","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2022.4.401","url":null,"abstract":"The largest number of currently produced drugs for the treatment of mastitis in lactating cows contain antimicrobial components (antibiotics) as active substances. The studies were carried out on Red-Motley Holstein cows, in which subclinical mastitis was diagnosed. The animals selected for the experiment were divided into three groups. The first group included 8 animals, they were not treated and served as a negative control. In the second group, 10 cows were selected for treatment with Biferon-B for three days once a day at a dose of 10 ml. per animal. The animals of the third group were simultaneously administered interferon-λ and BiferonB intramuscularly, 10 ml each during those days. According to the results of clinical studies, it was found that no recovery was recorded in the animals in the negative control group. In the group of animals where Biferon-B was used, the efficacy of treatment was 60.0%, and with the combined use of Biferon-B and interferon-λ, the effect of treatment was 83.3%. In these animals, the number of somatic cells decreased by 14.3 times, the concentration of neutrophils – by 2.6 times, the amount of total Ig - by 67.5%, CIC - by 61.5%, against the background of an increase in the number of lymphocytes by 6.0 times, monocytes - by 2.1 times and lysozyme - by 45.3%, and the bacterial contamination of the udder secretion also decreased to 0.08 thousand CFU/ml, which was by 120 times less compared to the initial state. Only Escherichia coli has been isolated from the udder secretion in 20.0% of cases that confirms the clinical efficacy of the treatment.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78553941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.52419/issn2072-2419.2022.4.374
M. Lapina, G. Kovaleva, N. Sulyga, V. Vitol
In recent years, there has been a tendency to reduce the age of the first insemination of replacement heifers of dairy breeds. However, calving at an early age is often accompanied by complications, which lead to stillborn offspring. In order to reduce the risk of a pathological calving, it is recommended to use stud bulls with an index value of “calving ease” of no more than 2.2 for insemination of heifers of dairy breeds, but there are no objective data on the effect of this index on birth defects. The purpose of our research was to study the calving ease and the stillbirth in offspring of first-calf heifers of three age groups, which were inseminated by bulls with an index of “calving ease” of 1.6 and 1.8-2.1. The difference in the number of difficult calving in favor of first-calf heifers, which were inseminated by stud bulls with an index of “calving ease” of 1.6, was 8.7%. The greatest difference between the experimental groups was at the first insemination at the age of 12-13 months – 29.2%. With an increase in the age of the first insemination, the difference between the experimental groups decreased and amounted to 11.3% at the age of 14-15 months. The minimum difference between the experimental groups was when animals were inseminated at the age of 16-18 months – 5.2%. From using stud bulls with an index of “calving ease” of 1.6, they obtained 9.7% of stillborn offspring, while with an index of “calving ease” of 1.8-2.1 – 17.7%. As for age subgroups, the difference in favor of the animals of the first experimental group, in comparison with the second experimental group, was 5.5%, 4.5% and 10.8%, respectively. The greatest number of difficult calving and stillborn offspring was registered with the first insemination of experimental animals at the age of 12-13 months. This correlation was typical for both the first and second experimental groups.
{"title":"Birth defects in first-calf heifers with the use of stud bulls with different index values of “calving ease”","authors":"M. Lapina, G. Kovaleva, N. Sulyga, V. Vitol","doi":"10.52419/issn2072-2419.2022.4.374","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2022.4.374","url":null,"abstract":"In recent years, there has been a tendency to reduce the age of the first insemination of replacement heifers of dairy breeds. However, calving at an early age is often accompanied by complications, which lead to stillborn offspring. In order to reduce the risk of a pathological calving, it is recommended to use stud bulls with an index value of “calving ease” of no more than 2.2 for insemination of heifers of dairy breeds, but there are no objective data on the effect of this index on birth defects. The purpose of our research was to study the calving ease and the stillbirth in offspring of first-calf heifers of three age groups, which were inseminated by bulls with an index of “calving ease” of 1.6 and 1.8-2.1. The difference in the number of difficult calving in favor of first-calf heifers, which were inseminated by stud bulls with an index of “calving ease” of 1.6, was 8.7%. The greatest difference between the experimental groups was at the first insemination at the age of 12-13 months – 29.2%. With an increase in the age of the first insemination, the difference between the experimental groups decreased and amounted to 11.3% at the age of 14-15 months. The minimum difference between the experimental groups was when animals were inseminated at the age of 16-18 months – 5.2%. From using stud bulls with an index of “calving ease” of 1.6, they obtained 9.7% of stillborn offspring, while with an index of “calving ease” of 1.8-2.1 – 17.7%. As for age subgroups, the difference in favor of the animals of the first experimental group, in comparison with the second experimental group, was 5.5%, 4.5% and 10.8%, respectively. The greatest number of difficult calving and stillborn offspring was registered with the first insemination of experimental animals at the age of 12-13 months. This correlation was typical for both the first and second experimental groups.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80514083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-03DOI: 10.52419/issn2072-2419.2022.4.415
D. F. Valiullina, D. Morozova, D. R. Amirov
Currently, veterinary specialists use in clinical practice modern, minimally invasive and suitable methods for determining and detecting pathologies of bone mineralization, one of which is densitometry. Bone mineralization density was determined by radiography and subsequent analysis of radiographs using the HiScene program. The obtained results of digital processing of radiographic images in puppies of the control and experimental groups on radiographs of the pelvic bones showed the absence of destructive changes in bone and cartilage tissues. The shape and size of the pelvic bones are normal, the contours are clear, even, the bone architectonics is not changed, there were no signs of arthrosis of the hip joints. On x-rays of the hip joints, the nuclei of ossification of the heads (left and right) of the femurs were not visualized – the age norm, they were rounded, symmetrical, centered in the joints. Assessment of changes in bone mineral density was carried out by densitometry (determination of the density of materials by checking their resistance to the passage of X-rays) in puppies aged 6 to 9 months, subjected to ovariohysterectomy (OHEC) and without surgery. As a result of the research, the authors found that in animals, against the background of ovariohysterectomy (experimental group), there is a slow increase in the mineral density of the bone tissue of the pelvis and femur compared to the control group. According to the authors, it is associated with the maturation of puppies and with the intensive growth of the skeleton. The accumulation of the mineral component in animals subjected to OHEC is confirmed by the difference between the indices of the groups (≈9.3%). This fact allows us to conclude that OHEC has a negative effect on bone mineralization.
{"title":"Effect of ovariohysterectomy on pelvic mineral density in puppies","authors":"D. F. Valiullina, D. Morozova, D. R. Amirov","doi":"10.52419/issn2072-2419.2022.4.415","DOIUrl":"https://doi.org/10.52419/issn2072-2419.2022.4.415","url":null,"abstract":"Currently, veterinary specialists use in clinical practice modern, minimally invasive and suitable methods for determining and detecting pathologies of bone mineralization, one of which is densitometry. Bone mineralization density was determined by radiography and subsequent analysis of radiographs using the HiScene program. The obtained results of digital processing of radiographic images in puppies of the control and experimental groups on radiographs of the pelvic bones showed the absence of destructive changes in bone and cartilage tissues. The shape and size of the pelvic bones are normal, the contours are clear, even, the bone architectonics is not changed, there were no signs of arthrosis of the hip joints. On x-rays of the hip joints, the nuclei of ossification of the heads (left and right) of the femurs were not visualized – the age norm, they were rounded, symmetrical, centered in the joints. Assessment of changes in bone mineral density was carried out by densitometry (determination of the density of materials by checking their resistance to the passage of X-rays) in puppies aged 6 to 9 months, subjected to ovariohysterectomy (OHEC) and without surgery. As a result of the research, the authors found that in animals, against the background of ovariohysterectomy (experimental group), there is a slow increase in the mineral density of the bone tissue of the pelvis and femur compared to the control group. According to the authors, it is associated with the maturation of puppies and with the intensive growth of the skeleton. The accumulation of the mineral component in animals subjected to OHEC is confirmed by the difference between the indices of the groups (≈9.3%). This fact allows us to conclude that OHEC has a negative effect on bone mineralization.","PeriodicalId":14419,"journal":{"name":"International Journal of Veterinary Medicine","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85323973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：