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Cytogenetics and Importance of Genetic Counselling in Recurrent Pregnancy Losses: Experience from Tertiary Care Laboratory 细胞遗传学和遗传咨询在复发性妊娠丢失中的重要性:来自三级保健实验室的经验
Pub Date : 2017-10-31 DOI: 10.21013/jas.v9.n1.p2
Shailesh Pande, A. Pais, Gauri N. Pradhan, Yamini Jadhav, Chaitali Parab, Bharat Kalthe, S. Matkar
Loss of pregnancy either naturally or by medical termination is a destructive experience to the couple, especially those experiencing recurrent pregnancy losses (RPL). It is important to rule out the genetic aspect as the cause of pregnancy wastages. This retrospective study aimed to determine the frequency of chromosomal abnormalities and its various cytogenetic types in the samples received by Metropolis Healthcare laboratory, Mumbai. This study was conducted on the samples referred for chromosomal karyotyping with a history of Bad Obstetric History (BOH). The couples who had an experience of two or more pregnancy losses were included in this study. Out of the 2102 samples referred, chromosomal abnormality was recorded in 384 (18.27%) cases. Out of chromosomal abnormal cases, 126 (5.99%) patients had reciprocal translocations out of which 27 (21.43%) were Robertsonian translocations. Inversion of chromosome 9 was seen in 81 (21.09%) patients, while inversion Y in 28 (7.29%) patients,  and polymorphic variation like increase in length of satellite or heterochromatic region recorded in almost 149 (38.30%) patients. Cytogenetic evaluation of couples with recurrent pregnancy losses (RPL) is very important as after knowing the parental chromosomal pattern appropriate counseling can be offered to know the risk of recurrence, option of prenatal diagnosis and also opens the option of reproduction in some cases. This will also help them to have a cytogenetically healthy baby. Since the cytogenetic abnormalities are usually familial, the close blood relatives may also be benefited once the abnormality is detected.
无论是自然流产还是医疗终止妊娠,对夫妇来说都是一种破坏性的经历,尤其是那些反复流产的夫妇。重要的是要排除遗传因素作为妊娠浪费的原因。本回顾性研究旨在确定孟买大都会卫生保健实验室收到的样本中染色体异常及其各种细胞遗传学类型的频率。本研究对有不良产科史(BOH)的患者进行染色体核型分析。有两次或两次以上流产经历的夫妇被纳入这项研究。在2102例样本中,染色体异常384例(18.27%)。染色体异常126例(5.99%)为反向易位,其中罗伯逊易位27例(21.43%)。9号染色体反转81例(21.09%),Y染色体反转28例(7.29%),卫星区或异色区长度增加等多态性变异149例(38.30%)。反复流产夫妇的细胞遗传学评估非常重要,因为在了解父母染色体模式后,可以提供适当的咨询,以了解复发的风险,产前诊断的选择,在某些情况下还可以选择生育。这也将有助于他们有一个细胞遗传学健康的婴儿。由于细胞遗传学异常通常是家族性的,一旦发现异常,近亲也可能受益。
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引用次数: 0
Determination of Some Mechanical and Thermal Properties of Cattle Manure Pellets 牛粪颗粒若干力学和热性能的测定
Pub Date : 2017-10-23 DOI: 10.21013/JAS.V9.N1.P1
Javad Rezaeifar, M. Pourjafar
One of the important subsidiary materials of livestock units is excreta. The livestock waste (including the combination of the Cattle Manure and products case in the bed) as a valuable source of the minerals (Nitrogen 1.78 % , phosphor us 0.5% and potassium 0.99%).For the transportation of the cattle manure in the natural state is difficult and costly due to being low the special mass and the nature of mass. It is produced around 6 million thons the cattle manure yearly in Iran which it is usually filled in the place according to the lack of organic materials of the soils country, the use of the cattle manure causes increasing the productivity of crops. We usually need to some of the mechanical and thermal properties of the cattle manure for producing the pellet by Extruder, so we Survey some of the mechanical and thermal properties of the cattle manure pellet in this research such as the shear stress and the coefficient of thermal diffusivity cattle manure. We obtain the coefficient of the thermal diffusivity manure (
牲畜单位的重要附属物质之一是排泄物。畜禽粪便(包括床上的牛粪和产品的组合)是矿物质(氮1.78%,磷0.5%和钾0.99%)的宝贵来源。由于天然状态下牛粪的特殊质量和质量性质低,运输难度大,成本高。伊朗每年生产大约600万加仑的牛粪,由于土壤缺乏有机物质,牛粪通常被填充在这个地方,使用牛粪可以提高作物的生产力。为了利用挤出机生产球团,通常需要了解牛粪的一些力学和热性能,因此本研究对牛粪球团的一些力学和热性能进行了研究,如牛粪的剪切应力和热扩散系数。我们得到了热扩散系数(
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引用次数: 0
Application of Fuzzy Logic in Agricultural Systems: A Review 模糊逻辑在农业系统中的应用综述
Pub Date : 2017-10-23 DOI: 10.21013/jas.v8.n3.p1
M. Pourjafar, M. Mazloomzadeh
Fuzzy logic is a development of Boolean logic dealing with the meaning of sectional truth. Whiles classical logic holds that everything can be expressed in double terms (0 or 1, black or white, yes or no). Fuzzy logic permits the use of linguistic values of variables and ambiguous relationships for modeling system treatment and it is a strong concept for handling time-varying, non-linear and adaptive systems. Because of need to improving agricultural systems and enhancing the farmer's income, the current study presents a review of fuzzy logic literature, applications to agricultural systems and fuzzy modeling techniques. One example has been expressed the steps of building a fuzzy model and consider the strategies for improving of agricultural systems.
模糊逻辑是布尔逻辑的发展,它处理的是部分真值的含义。而经典逻辑认为,一切都可以用双项表示(0或1,黑或白,是或否)。模糊逻辑允许使用变量的语言值和模糊关系来建模系统处理,它是处理时变、非线性和自适应系统的强大概念。基于改进农业系统和提高农民收入的需要,本文综述了模糊逻辑的相关文献、模糊逻辑在农业系统中的应用以及模糊建模技术。举例说明了建立模糊模型并考虑农业系统改进策略的步骤。
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引用次数: 3
Chemical Composition of Some Natural Palm Wine Preservatives 几种天然棕榈酒防腐剂的化学成分
Pub Date : 2017-10-10 DOI: 10.21013/JAS.V08.N03.P01
Ngounou Batchanji Steve, Feumba Dibanda Romelle, Yadang Germaine, Nyobe Emillienne Carine, Achegui Nestor, Ndanga Thomas, Medoua Nama Gabriel
Palm wine is the commonest name of the beverage obtained from fermented palm sap, the exudate from tapped unopened spathe of oil palm tree (Elaeis guineensis). This refreshing wine of West and Central Africa is very sweet but within 24 hours the concentration of sucrose falls to less than 50% the initial amounts due to a rapid sugar fermentation by microorganisms. In Cameroon, Central Africa, traditional attempts to preserve palm wine imply the introduction of natural preservatives mostly barks and leaves from edible or medicinal plants. The leaves of Cymbopogon citratus, Pimenta racemosa, Vernonia amygdalina, Ocimum basilicum and the barks and the leaves of Garcinia lucida and of Adansonia digitata commonly used in palm wine preservation were analyzed for their chemical composition. The results showed that lipid, protein, ash, crude fiber, total sugars and vitamin C contents were respectively from 15.70 ± 1.13 to 23.57 ± 1.80%, from 8.38 ± 0.38 to 43.81 ± 3.69%, from 1.14 ± 0.17 to 10.06 ± 0.39%, from 11.74 ± 1.11 to 48.42 ± 0.55%, and from 1.49 ± 0.02 to 22.56 ± 0.59% and from 55.76 ± 3.15 to 175.71 ± 2.09 μg /100 g dw. The phyto-chemicals levels were respectively from 1.37 ± 0.22 to 3.61 ± 0.6 % for total alkaloids, 2.66 ± 0.12 to 4.80 ± 0.24 % for total phenolics and 0.57 ± 0.09 to 1.88 ± 0.09 mg /100 g dw for total saponins. Concerning anti-nutrients, the oxalates levels were higher than the threshold value (250 mg/100 g) reported as safety limit. Despise the proven antimicrobial benefits of alkaloids, saponins and phenolics found in those leaves and barks, caution shall be paid during their use due to their high oxalates levels.
棕榈酒是最常见的饮料,从发酵棕榈汁中获得,从油棕树(Elaeis guineensis)未打开的芽中渗出。这种产于西非和中非的清爽葡萄酒非常甜,但在24小时内,由于微生物的快速糖发酵,蔗糖浓度下降到初始量的50%以下。在中非的喀麦隆,保存棕榈酒的传统尝试意味着引入天然防腐剂,主要是可食用或药用植物的树皮和叶子。对棕榈酒保鲜常用的香槟酒、总状红桃、苦杏仁、罗勒叶和透明藤黄、水仙叶的化学成分进行了分析。结果表明:脂肪、蛋白质、灰分、粗纤维、总糖和维生素C含量分别为15.70±1.13 ~ 23.57±1.80%、8.38±0.38 ~ 43.81±3.69%、1.14±0.17 ~ 10.06±0.39%、11.74±1.11 ~ 48.42±0.55%、1.49±0.02 ~ 22.56±0.59%和55.76±3.15 ~ 175.71±2.09 μg /100 g dw。总生物碱含量为1.37±0.22 ~ 3.61±0.6%,总酚含量为2.66±0.12 ~ 4.80±0.24%,总皂苷含量为0.57±0.09 ~ 1.88±0.09 mg /100 g dw。在抗营养素方面,草酸盐含量高于安全限值(250 mg/100 g)。轻视在这些叶子和树皮中发现的生物碱、皂苷和酚类物质的抗菌作用,由于它们的草酸盐含量高,在使用过程中应谨慎。
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引用次数: 0
Determination of Some Physical Properties of Dried Jujube Fruit 枣干果实若干物理性质的测定
Pub Date : 2017-09-01 DOI: 10.21013/JAS.V8.N2.P1
M. Pourjafar
Some physical properties of Iranian Jujube fruit were evaluated. Jujube fruit samples mean values of length, width, thickness were found as 18.005mm, 14.96 mm, 15.26 mm. Arithmetic mean diameter, geometric mean diameter were found as 16.08mm, 16.01 respectively and surface area, Sphericity, porosity, mass, volume, bulk density and true density were also found as: 805.65 mm 2 ,89%, 77.2%, 1.45gr, 2.61 cm 3 , 0.23gr cm -3 , 0.55gr cm -3 respectively. Projected area perpendicular to length, width and thickness were measured as, 152.03 mm 2 , 220.02 mm 2 , 271.4mm 2 respectively. With comparison between friction coefficients on different surface for Jujube fruit. The results showed that the static coefficient of friction was more than dynamic coefficient of friction.The tests done on the galvanized steel surface, plywood and glass surface. The most coefficient of friction was found on the plywood surface and the least coefficient of friction was measured on the glass surface.
对伊朗枣果实的一些物理特性进行了评价。红枣果实样品的长、宽、厚均值分别为18.00mm、14.96 mm、15.26 mm。算术平均直径、几何平均直径分别为16.08mm、16.01,比表面积、球度、孔隙度、质量、体积、容重、真密度分别为805.65 mm 2、89%、77.2%、1.45gr、2.61 cm 3、0.23gr cm -3、0.55gr cm -3。垂直于长度、宽度和厚度的投影面积分别为152.03 mm 2、220.02 mm 2、271.4mm 2。对红枣果实不同表面摩擦系数进行了比较。结果表明:静摩擦系数大于动摩擦系数;试验在镀锌钢板表面、胶合板表面和玻璃表面进行。胶合板表面的摩擦系数最大,玻璃表面的摩擦系数最小。
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引用次数: 0
Quantitative Activity Structure Relationship (QSAR) of a Series of Azetidinones Derived from Dapsone by the Method of Density Functional Theory (DFT) 密度泛函理论(DFT)方法研究氨苯砜衍生的一系列氮杂二酮的定量活性结构关系
Pub Date : 2017-09-01 DOI: 10.21013/JAS.V8.N2.P2
Jean Stéphane N’dri, M. Koné, C. Kodjo, S. T. Affi, Ahmont Landry Claude Kablan, O. Ouattara, Doh Soroand, N. Ziao
This QSAR study, which involved a series of Azetidinones derived from 4,4'-diaminodiphenylsulfone (dapsone), yielded two models based on molecular descriptors and the antibacterial activities Escherichia coli and Staphylococcus aureus.The molecular descriptors were obtained by applying the methods of quantum chemistry at the B3LYP/6-31G (d) level. The statistical indicators of the first model which is a function of the Escherichia coli activity are: the coefficient of determination R 2 equals 0.992, the standard deviation S equals 0.342, the Fischer coefficient F equals 185.088 and the cross-validation coefficient Q 2 CV equals 0.992. Those of the second model showing the activity of Staphylococcus aureus are: the regression coefficient R 2 = 0.987, a standard deviation S=0.193, the Fischer coefficient F=114.955 and the cross-validation coefficient Q 2 CV = 0.987. These models have good statistical performances. The quantum descriptors of dipole moment (μ), global softness (σ) and electronegativity (χ) are responsible of the antibacterial activity of the Azetidinones derived from dapsone. In addition, the dipole moment is the priority descriptor for the prediction of the antibacterial activity of the studied compounds. The Eriksson et al. acceptance criteria used for the test set is verified. The values of the dtheo/dexp ratio of the theoretical and experimental activities for the test set tend towards unity.
本QSAR研究涉及一系列由4,4'-二氨基二苯砜(氨苯砜)衍生的氮杂二酮,基于分子描述符和抗菌活性建立了两个模型大肠杆菌和金黄色葡萄球菌。应用量子化学方法在B3LYP/6-31G (d)水平上获得了分子描述符。第一个模型的统计指标为:决定系数r2 = 0.992,标准差S = 0.342, Fischer系数F = 185.088,交叉验证系数q2 CV = 0.992。第二个金黄色葡萄球菌活性模型的回归系数r2 = 0.987,标准差S=0.193, Fischer系数F=114.955,交叉验证系数q2 CV = 0.987。这些模型具有良好的统计性能。偶极矩(μ)、整体柔软度(σ)和电负性(χ)的量子描述子对氨苯砜衍生物的抑菌活性起重要作用。此外,偶极矩是预测所研究化合物抗菌活性的优先描述符。验证了Eriksson等人用于测试集的验收标准。理论活动和实验活动对测试集的dtheo/dexp比值值趋于一致。
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引用次数: 7
Analysis of Buccal Cortical Plate Thickness for Placement of Mini-Implants – A CBCT Study 植入微型种植体时颊皮质板厚度的分析- CBCT研究
Pub Date : 2017-08-15 DOI: 10.21013/JAS.V8.N1.P3
R. David, Raghu Ranjan, R. DineshM, M. Dharmar, C. AmaranthB, Sharmila Arjunan
The purpose of this study was to quantitatively evaluate buccal cortical bone thickness in the maxilla and mandible so as to provide guidelines for mini-implant placement. Cone beam computed tomographic scans of thirty patients (Age 16-30 years) were collected from preorthodontic records. Each measurement area was coded with a number beginning in the maxillary right quadrant distal to the second molar with number 1 and ending in the mandibular right quadrant distal to the second molar with number 30. For analysis these measurement areas were also grouped as sextants. Three measurement points were then defined at 2 mm, 4 mm and 6 mm from the alveolar crest in each measurement. The measuring locations were recorded on the four quadrants, resulting in 90 locations in the buccal cortical plate of maxillary and mandibular arch. The region of interest was explored at each measuring location of the 30 sites using Galileos software. The optimal site for miniimplant placement in the anterior region is between the central and lateral incisors in the maxilla and between the lateral incisor and the canine in the mandible at the 6-mm level from the alveolar crest. At the buccal aspect of the posterior region of both jaws, the optimal sites are between between the first and second molars i.e. in the Maxillary arch at 4 mm level on the right side and left side and in the Mandibular arch at 6 mm level on the right side and left side.
本研究的目的是定量评估上下颌颊皮质骨厚度,为微型种植体的植入提供指导。从正畸前记录中收集了30例患者(年龄16-30岁)的锥形束计算机断层扫描。每个测量区域都用数字编码,从第二磨牙远端的上颌右象限开始,编号为1,到第二磨牙远端的下颌右象限结束,编号为30。为了便于分析,这些测量区域也被归类为六分仪。然后在每次测量中分别在距牙槽嵴2 mm、4 mm和6 mm处定义三个测量点。在四个象限上记录测量位置,得到上颌弓颊皮质板的90个位置。使用伽利略软件在30个站点的每个测量位置探索感兴趣的区域。在前牙区放置微型种植体的最佳位置是在上颌骨的中门牙和侧门牙之间以及下颌骨的侧门牙和犬齿之间距离牙槽嵴6毫米的水平。在双颌后区颊面,最佳位置在第一磨牙和第二磨牙之间,即在左右两侧的上颌弓4毫米水平处和左右两侧的下颌弓6毫米水平处。
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引用次数: 0
Adsorption Study of the Removal of Copper (II) Ions using Activated Carbon Based Canarium Schweinfurthii Shells Impregnated with ZnCl2 浸渍ZnCl2的活性炭基Canarium Schweinfurthii壳对铜(II)离子的吸附研究
Pub Date : 2017-08-15 DOI: 10.21013/JAS.V8.N1.P2
Kammegne Adelaide Maguie, N. J. Nsami, Kouotou Daouda, Che Nangah Randy, K. J. Mbadcam
The adsorption of Cu2+ions on activated carbon based canarium schweinfurthii impregnated with ZnCl2 was studied. The shells of canarium schweinfurthii were impregnated with ZnCl2 at varying ZnCl2 concentrations, temperature, residence time while keeping the heating rate fixed at 10 °C / min and the ratio of impregnation of 1:1. The activated carbon with the highest surface area in term of iodine number of 860,817 mg/g, the highest methylene blue of 741,6 mg/g and 74,66 % of yield of carbon was obtained at 650 °C, 60 % ZnCl 2 and 30min. From the batch adsorption studies, the equilibrium time was found to be 40 min. Analysis of equilibrium isotherm models revealed a good correlation of the experimental data with the Tempkin (R 2 = 0.909) model. This confirms a high affinity of the activated carbon for Cu 2+ ions on the heterogeneous surface. The value of energy obtained from the Tempkin model was 60,606 J/mol and the presence of pics between 487 cm-1 to 871 cm-1 indicating that physisorption and chemisorption were taking place during this sorption. The pseudo-second order kinetics(R 2 = 0.999) governs the adsorption of Cu2+ions on this activated carbon.
研究了氯化锌浸渍活性炭基施韦因富氏canarium对Cu2+离子的吸附性能。在升温速率为10℃/ min、浸渍比为1:1的条件下,采用不同浓度、不同温度、不同浸渍时间的ZnCl2对施韦因氏鸭壳进行浸渍。在650℃、zncl2含量为60%、反应时间为30min的条件下,所得活性炭的比表面积为860,817 mg/g,亚甲基蓝为741,6 mg/g,收率为74.66%。平衡等温线模型分析表明,实验数据与Tempkin模型(r2 = 0.909)具有良好的相关性。这证实了活性炭对非均相表面cu2 +离子的高亲和力。从Tempkin模型得到的能量值为60606 J/mol,在487 ~ 871 cm-1之间存在光子,表明在吸附过程中发生了物理吸附和化学吸附。拟二级动力学(r2 = 0.999)决定了该活性炭对Cu2+离子的吸附。
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引用次数: 4
Generation of a Chimeric Antibody against a Synthetic Peptide Derived From IL-23p19 with Potential Therapeutic Application IL-23p19合成肽嵌合抗体的产生及其潜在的治疗应用
Pub Date : 2017-08-15 DOI: 10.21013/JAS.V8.N1.P1
Pérez-Etcheverry Diana, L. Carmen
Aim : To construct an express a mouse-human chimeric antibody against IL-23p19 using a synthetic peptide as immunogen. Methods : Immunization of mice with a synthetic peptide derived from the IL-23p19 sequence and generation of hybridoma secreting specific antibodies. The chimeric antibody was created using two eukaryotic plasmid constructions; one of them carrying the light mouse-human chain and the other the heavy mouse-human chain. CHO-K1 cells were cotransfected with both plasmids and stable transfectants were grown in selective culture medium. Results : A chimeric version of anti-IL-23p19 was successfully constructed and expressed in eukaryotic cells. The expressed chimeric antibody showed specific recognition not only of the peptide used as immunogen but also the subunit p19 and the complete interleukin Il-23. Conclusion : A Chimeric antibody that was developed against a synthetic peptide, which is able to recognize the parent protein IL23 biologically active, could be developed into a targeted therapy in diseases with chronic inflammation.
目的:利用合成的肽段作为免疫原,构建表达人-鼠嵌合抗IL-23p19抗体。方法:用IL-23p19合成肽段免疫小鼠,产生分泌特异性抗体的杂交瘤。利用两个真核质粒构建嵌合抗体;其中一个带着轻的鼠人链,另一个带着重的鼠人链。用两种质粒共转染CHO-K1细胞,并在选择性培养基中培养稳定的转染物。结果:成功构建抗il -23p19嵌合蛋白并在真核细胞中表达。所表达的嵌合抗体不仅能特异性识别作为免疫原的肽,还能识别亚基p19和完整的白细胞介素Il-23。结论:合成的嵌合抗体能够识别具有生物活性的亲本蛋白il - 23,可用于慢性炎症疾病的靶向治疗。
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引用次数: 0
Role of Local Building Materials on the Energy Behaviour of Habitats in Ouagadougou 瓦加杜古当地建筑材料对栖息地能源行为的作用
Pub Date : 2017-08-10 DOI: 10.21013/JAS.V8.N2.P3
A. Compaoré, B. Ouédraogo, H. Guengane, Etienne Malbila, D. Bathiébo
The present work is a contribution to the energy efficiency in the habitats by the valorization of the local building materials in Burkina Faso. A comparative numerical study on the thermal behavior and energy consumption of some habitats in local and modern building materials is carried out. The simulations were carried out using computer code written in FORTRAN language over a period of one year. Analysis of the results shows that habitats in local construction materials (BTC, BLT, adobe) have a better thermal response compared to modern building materials (hollow cinder block). Generally speaking, the temperature and humidity profiles obtained do not make it possible to ensure the summer comfort in these buildings throughout the year. The evaluation of air-conditioning loads to ensure thermal comfort in these habitats shows that they are higher respectively in the blocks, BLT, BTC, adobe constructions. We therefore consider that local building materials represent a good alternative for the search for energy efficiency in buildings.
目前的工作是通过布基纳法索当地建筑材料的增值,为栖息地的能源效率做出贡献。本文对当地和现代建筑材料的一些生境的热行为和能耗进行了数值比较研究。模拟是在一年的时间里用FORTRAN语言编写的计算机代码进行的。分析结果表明,与现代建筑材料(空心煤渣砖)相比,当地建筑材料(BTC, BLT,土坯)的栖息地具有更好的热响应。一般来说,获得的温度和湿度分布并不能保证这些建筑物全年的夏季舒适。空调负荷的热舒适性评价结果表明,砌块建筑、BLT建筑、BTC建筑和土坯建筑的空调负荷均较高。因此,我们认为本地建筑材料是寻求建筑物能源效率的一个很好的选择。
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引用次数: 8
期刊
IRA-International Journal of Applied Sciences
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