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Molecular Method to Diagnosis of some Strongylide Nematode of goats in Nyala Area South Darfur State- Sudan 苏丹南达尔富尔州尼亚拉地区山羊类圆线虫的分子诊断方法
Pub Date : 2017-05-01 DOI: 10.9790/2380-1004025456
S. Hassan
A total of 100 positive faecal samples were examined using floatation technique to detect strongylide eggs, were confirmed by PCR technique, faecal samples were storage by frozen in -20 ° C and DNA extraction by using phenol/chloroform/isoamylalcohol protocol. PCR leading to amplify the region of the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA (r DNA) of strongylide species, amplification by three different primers in separate reactions, in each reaction used species specific primer forward and a universal reverse primer to srongylide species NC2. Haemonchus contortus, Trichostrongylus spp and Oesophagostomum columbianum prevalence rates were recorded as 47(47%), 4(4%) and (0%) respectively, Most commonly, small ruminants are affected by multiple strongylide nematodes, mixed infection between Haemonchus contortus and Trichostrongylus spp was recorded 3(3%) Keyword: Strongylide, PCR, ITS-2, NC2, goats, Sudan.
采用浮法检测100例阳性粪便标本,采用PCR技术鉴定,-20℃冷冻保存,采用苯酚/氯仿/异戊醇提取DNA。聚合酶链反应(PCR)对强酰基内酯种核糖体DNA (r DNA)第二段内转录间隔区(ITS-2)进行扩增,通过三种不同的引物在不同的反应中进行扩增,每种反应使用物种特异性引物正向和通用反向引物对强酰基内酯种NC2进行扩增。弯血蜱、毛圆线虫和柱状食道线虫的患病率分别为47(47%)、4(4%)和0(0%),最常见的是小反刍动物感染多种圆形线虫,弯血蜱和毛圆线虫混合感染3(3%)。关键词:圆形线虫,PCR, ts -2, NC2,山羊,Sudan。
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引用次数: 0
The Economic Efficiency of Wheat Farms in Baghdad Province (Abu-Ghraib), (In Iraq - 2015) 巴格达省小麦农场经济效益(Abu-Ghraib),(伊拉克- 2015)
Pub Date : 2017-05-01 DOI: 10.9790/2380-1005014046
H. Mohammed
Wheat is considered as a strategic crop and it occupies economic position in most of the world countries due to its nutrition importance ,it is a food resource of more than 35% of world population and it is considered the most importance cereal crop that covered the largest area grown by wheat on the earth surface .in spite of its importance, wheat production suffers a clear turbulence because farmers don’t go on planting wheat due to the gated net return reduction resulted by not using the new technical and scientific tools in farming besides bad management which is considered in general as the important limitation factor in agriculture in Iraq and in wheat cultivation in specific case. ,Abu-Graib region was chosen as an applicable model in this study. The data was collected by using questionnaires that were given to 72 farmers in AbuGhraib region in agricultural season 2014-2015 , and it formed 3.42% of wheat farms in the studied society and 2103 farmers . The research subjected analysis of revenues and costs items to study the economic efficiency by application of economic efficiency criterias and determine the technical efficiency on concept of the randomized border analysis after determination of the production logarithmic function by using Frontier program. , The result showed that all the farms achieved valuable economic income when the economic efficiency criterias were applied .Results showed values ,(profit , income net, farming return to Labor returns to farming management incomes and Benefit Cost ratio were ( 492246.88 , 470825.88 , 462078.8 , 29568.3 and 4.6 ) thousand dinars respectively.The best period to recover the capital was in the area farm group 31 donums and more, It reached to 0.20 .at break – even point was 16.12 tons and there was positive relation between rising income level and land area .The technical efficiency was determined according to stochastic frontier analysis model after determinations of transcendence logarithmic function by using Frontier program which pointed that the work and the capital had positive effect on yield production quantity when their increase in 1% ratio resulted to increase production to 0.36% and 0.64% respectively. The technical efficiency was 99%and this means that the farmers can increase the production to 1% without increase in the used economic resources.
小麦被认为是一种战略作物,由于其营养的重要性,它在世界上大多数国家都占有经济地位,它是世界上35%以上人口的食物资源,它被认为是最重要的谷类作物,它在地球表面上覆盖的面积最大。小麦生产遭受了明显的动荡,因为农民不再继续种植小麦,这是由于没有使用新的技术和科学的耕作工具而导致的门控净收益减少,而管理不善通常被认为是伊拉克农业的重要限制因素,特别是在小麦种植方面。,选择阿布-格莱布地区作为本研究的适用模型。数据通过2014-2015年农季对AbuGhraib地区72名农民进行问卷调查收集,占研究社会小麦农场的3.42%,农户2103人。本研究对收益和成本项目进行分析,应用经济效率标准研究经济效率,并利用Frontier程序确定生产对数函数后,在随机边界分析的概念上确定技术效率。结果表明,采用经济效益标准后,所有农场均获得了有价值的经济收入,利润、净收入、农业劳动报酬、农业经营报酬和效益成本比分别为(492246.88、470825.88、462078.8、29568.3和4.6)千第纳尔。恢复资金的最佳时期是在农场群31个月以上的地区。收入水平的提高与土地面积呈正相关。采用frontier程序确定超越对数函数后,根据随机前沿分析模型确定技术效率,指出劳动和资本每增加1%的比例,产量分别提高到0.36%和0.64%,对产量生产数量有正影响。技术效率为99%,这意味着农民可以在不增加经济资源使用的情况下将产量提高到1%。
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引用次数: 0
Studies on Amyloodinium Infestation in European Seabass (Dicentrarchuslabrax.) Fishes with Special Reference for Treatment. 欧洲鲈鱼(Dicentrarchuslabrax.)中淀粉菌侵染的研究有特殊参考价值的鱼。
Pub Date : 2017-05-01 DOI: 10.9790/2380-1005022948
Samar Saad Mohamed Seoud, V. Zaki, G. Ahmed, Nevien K. Abd El-Khalek
Amyloodinium ocellatum, a dinoflagellate which causes one of the most serious diseases of marine aquaculture. It is one of the most important of the ectoparasitic protozoa. In this respect, the present study was aimed to investigate the occurrence of Amyloodiniosis among cultured European seabass (Dicentrarchus labrax) fishes. The present study was aimed to investigate the occurrence of Amyloodiniosis among cultured European seabass (Dicentrarchus labrax) fishes. This work was carried out on 1065 European seabass fishes of different life stages (546 fry-222 fingerling and 78 adults) which were collected in different seasons of the year. Cultured fish were collected from different marine farms in Egypt during the period from April 2015 to April 2016 and subjected to full clinical parasitological and histopathological examination studies. The interaction between the environmental stressors and Amyloodinium ocellatum Infestation were also analyzed. The ecological factors that affects Amyloodiniosis occurrence also be discussed in the present study. As well as examining the antiseptic activity of hydrogen peroxide against the ectoparasitic protozoan A. ocellatum as a preliminary trial for treatment. It was cleared that, the treatment with hydrogen peroxide with a concentration 20% in two doses 100ppm & 200ppm for two weeks was more effective, safe and excellent and that was confirmed through lower the load of parasitic infestation and recovery of infested fishes with no mortalities were recorded at the end of the treatment trial.
一种引起海洋水产养殖最严重疾病之一的鞭毛藻。它是最重要的体外寄生原生动物之一。在这方面,本研究旨在调查在养殖的欧洲鲈鱼(Dicentrarchus labrax)鱼类中淀粉样变性的发生情况。本研究的目的是调查欧洲鲈鱼(Dicentrarchus labrax)养殖鱼类中淀粉样变性的发生情况。本研究以不同季节采集的1065条不同生命阶段的欧洲鲈鱼(546条炸鱼-222条鱼种和78条成鱼)为研究对象。2015年4月至2016年4月,在埃及的不同海洋养殖场采集了养殖鱼,并进行了全面的临床寄生虫学和组织病理学检查。分析了环境胁迫因子与绒淀粉虫侵染的相互作用。本研究还讨论了影响淀粉样变发生的生态因素。同时研究了过氧化氢对体外寄生原生动物的杀菌活性,作为治疗的初步试验。结果表明,过氧化氢浓度为20%,分100ppm和200ppm两种剂量处理两周,更有效、更安全、更优秀,这一点通过降低寄生虫感染负荷和在处理试验结束时记录的受感染鱼类的恢复情况得到了证实。
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引用次数: 0
Genetic Diversity in Cultivars of Phaseolus vulgaris L.by using Biochemical Markers. 利用生化标记分析菜豆品种的遗传多样性。
Pub Date : 2017-05-01 DOI: 10.9790/2380-1005012933
M. Kapil, V. N. Nathar
SDS-PAGE was used to evaluate and characterize the patterns of seed storage proteins in eight cultivars of Common bean.Electrophorogram for each variety was scored for presence and absence of protein band, which was transformed into a binary data matrix.Jaccards similarity coefficient, generated to determine the genetic similarity among cultivars, it revealed that most of the cultivars were genetically distant from each other. .Eight cultivars of Phaseolus vulgaris L. were investigated for genetic divergence based on seed protein profile using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Based on seed proteins, eleven maximum band were observed in B4 (Beans French yellow )and three minimum number of protein bands in B1 (BB) .The highest similarity index was observed in cultivar B2(Surbhi) 0.909 and lowest in B4(Beans French Yellow) and B6(Laffa) 0.272 in both cultivars .The highest dissimilarity value found in B4 and B6 0.728 in both the cultivars and lowest value in B2 cultivar 0.09 The un-weighted paired group method with arithmetic averages (UPGMA) exhibited two clusters A and B which were grouped into four sub-clusters I,II,III and IV. In broader spectrum, the genotypes from various sourcesdiffered in grouping and it was difficult to establish relationship between origin and cluster pattern. Particular clusters were better for specific traits that are suggested to utilize in crop improvement program. It is concluded that SDS-PAGE analysis of common bean seed storage protein is useful for evaluation of genetic variability and cultivars identification that help in common bean breeding program.
利用SDS-PAGE技术对8个普通豆品种的种子贮藏蛋白进行了鉴定。对每个品种的蛋白带的存在和不存在进行电泳评分,并将其转换成二进制数据矩阵。利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)技术,对8个菜豆(Phaseolus vulgaris L.)品种的种子蛋白谱进行了遗传差异分析。基于种子蛋白质,11最大带观察B4 (bean法国黄色)和三个最小数量的蛋白质乐队B1 (BB)相似性指数最高,在品种B2 (Surbhi) 0.909, B4 (bean法国黄色)和最低B6 (Laffa) 0.272在这两个品种不同价值最高,B4和B6 0.728在品种和最小值在0.09 B2品种un-weighted配对组与算术平均法(UPGMA)展出A和B两个集群分组在更广的光谱范围内,不同来源的基因型在分组上存在差异,难以建立起源与聚类模式之间的关系。在作物改良计划中,建议利用特定性状的特定集群。综上所述,蚕豆种子贮藏蛋白的SDS-PAGE分析可用于遗传变异评价和品种鉴定,为蚕豆育种提供参考。
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引用次数: 0
Loan Repayment Capacity of Cooperative Farmers in Ohaukwu Local Government Area of Ebonyi State, Nigeria 尼日利亚埃邦伊州Ohaukwu地方政府地区合作社农民的贷款偿还能力
Pub Date : 2017-05-01 DOI: 10.9790/2380-1005014753
S. U. Nwibo, C. Nwakpu
Loan repayment is crucial for the long-term sustenance of the credit institutions. But despite the repayment abilities of borrowers, studies showed that the loan repayment capacities of cooperative farmers in Ohaukwu LGA of Ebonyi state seems not to have been empirically documented. Both multistage and purposive sampling techniques were employed to generate data from 80 cooperative farmers using questionnaire administered as interview schedule. Data collected were analysed using both descriptive statistics percentages and mean, and inferential statistics – principal component factor analysis. Results showed that about 65 % of the cooperative farmers were of producer cooperative society while others belongs to either consumer or thrift and saving cooperative societies. The cooperative farmers repaid about 78 % of the total amount loan obtained. The study identified nature of investment, farm yield, household size, health condition of household member, time of loan disbursement, interest rate of the loan, amount of loan obtained, collateral value, and repayment conditions as the factors influencing cooperative farmers loan repayment, while high interest rate, amount of loan obtained, late disbursement, natural hazard, high illiteracy among co-operator, diversion of fund to nonfarm venture, family size, low farm output/income, and weather vagaries were identified as constraints to loan repayment among cooperative farmers. Based on the findings, the study recommended that financial institutions should address the issue of inadequate credit extended to cooperative farmers by increasing credit supply to them as such will help to improve productivity and income generation.
贷款偿还对信贷机构的长期生存至关重要。但是,尽管借款人有还款能力,研究表明,Ebonyi州Ohaukwu地方政府合作农民的贷款偿还能力似乎没有实证记录。采用多阶段抽样和目的抽样两种方法,对80名合作农户进行问卷调查。收集到的数据用描述性统计、百分比和平均值、推理统计-主成分因子分析进行分析。结果表明,约65%的合作社农民属于生产合作社,其他合作社农民属于消费合作社或节俭储蓄合作社。合作社农民偿还了约78%的贷款总额。研究确定投资的本质,农业产量、家庭规模、家庭成员健康状况,贷款支付,贷款的利率,贷款额度,抵押品价值,和还款条件影响农民合作贷款偿还的因素,利率高时,所获得的贷款,支付,自然灾害,co-operator文盲率高,非农转移的基金风险,家庭规模,农业产出/收入低,天气变化无常被认为是限制合作社农民偿还贷款的因素。根据调查结果,该研究建议金融机构应通过增加对合作社农民的信贷供应来解决向他们提供的信贷不足的问题,因为这将有助于提高生产力和创收。
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引用次数: 4
Epidemiology of Helminths in Cattle of Kirinyaga County, Kenya 肯尼亚基里尼亚加县牛中蠕虫流行病学研究
Pub Date : 2017-05-01 DOI: 10.9790/2380-100501106109
F. Njonge
The gastrointestinal tract (GIT) of animals harbor a variety of parasites particularly helminths, which causes clinical and sub-clinical parasitism. These parasites adversely affect the health status of animals and cause enormous economic losses to the livestock industry. The aim of this study was to determine the prevalence of helminths in cattle of Kirinyaga County. Epidemiological data was collected using a questionnaire survey administered to 244 respondents while rectal faecal samples from 261 cattle were collected within a period of four months to determine helminths egg counts. Sampling of herbage was carried out from 6 communal grazing sites for analysis of infective nematode larvae levels in the pasture. The cattle were weighed and blood collected by jugular venipuncture to determine the Total Protein (TP). The prevalence of GIN in the study area were species of Haemonchus (59%), Trichostrongylus (26%), Nematodirus (5%) and Oesophagostomum (10%). The most prevalent infective nematode larvae species in herbage was Haemonchus (55%) followed by species of Trichostrongylus (23.8%) and Oesophagostomum (13.4%). Trematodes species of Fasciola, Shistosoma and Paramphistomum were seen to be common in zebu cattle with Schistosoma showing the highest prevalence of 19%. The Total Protein (TP) on 60% of the sampled cattle were within the normal range of between 6 and 8%. However, 5% of the animals had low level of TP. The study shows the epidemiological profile of cattle helminth infections and the results form the basis for recommending the control strategies of helminths in this area.
动物胃肠道内寄生着多种寄生虫,尤其是蠕虫,引起临床和亚临床寄生。这些寄生虫对动物的健康状况产生不利影响,并给畜牧业造成巨大的经济损失。本研究的目的是确定蠕虫在Kirinyaga县牛中的流行程度。通过对244名应答者进行问卷调查收集流行病学数据,同时在4个月内收集261头牛的直肠粪便样本,以确定蠕虫卵数。对6个公共放牧区的牧草进行采样,分析牧场的感染性线虫幼虫水平。称重,颈静脉穿刺采血,测定总蛋白(TP)含量。研究区GIN的流行种为血蜱(59%)、毛线虫(26%)、线虫(5%)和食道口(10%)。在牧草中最常见的感染性线虫幼虫种类是血蜱(55%),其次是毛线虫(23.8%)和食道口线虫(13.4%)。吸虫种类主要有片形虫、血吸虫和副血吸虫,其中以血吸虫感染率最高,为19%。60%的样本牛的总蛋白(TP)在6% - 8%的正常范围内。然而,5%的动物TP水平较低。该研究显示了牛蠕虫感染的流行病学概况,结果为推荐该地区的蠕虫控制策略提供了依据。
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引用次数: 7
A study on the Length- Weight Relationship and Relative Condition Factor in Glossogobius giuris found in River Singla in the Karimganj district of Assam, India. 印度阿萨姆邦Karimganj地区Singla河中Glossogobius giuris的长重关系及相关条件因子研究。
Pub Date : 2017-05-01 DOI: 10.9790/2380-1004026769
Satyajit Das, Masuk Ahmed Barbhuiya, Riazul Islam Barbhuiya, D. Kar
An attempt had been made to develop a comparative relationship between the length and the weight of Glossogobius giuris and also the Relative condition factor (Kn) collected from the river Singla which is the inlet of one of the biggest wetland, Sone Beel in the Karimganj district of Assam. During the study period the value of exponent ‘n’ in the equation W = cL n was found to be 1.47 for the species. The computed ‘t’ value indicated that the ‘n’ value of the species is significantly different from the expected value ‘3’ and hence the Cube Law W = cL n did not hold good in the case of Glossogobius giuris in the river. The correlation co-efficient (r) in Glossogobius giuris was found to be 1, indicating that there is high positive correlation between length and weight in the species. The relative condition factor (Kn = 1.523) remained greater than 1 for the species indicating their general well being to be good in the river.
在阿萨姆邦Karimganj地区最大的湿地之一Sone Beel的入口Singla河上收集了一项研究,试图在长和重之间建立一种比较关系,以及相对条件因子(Kn)。在研究期间,发现W = cL n方程中指数n的值为1.47。计算的t值表明该物种的n值与期望值3有显著差异,因此立方定律W = cL n不适用于该河流中舌鱼的情况。舌鱼的相关系数r为1,表明该物种的长度与体重之间存在高度正相关关系。相对条件因子(Kn = 1.523)均大于1,表明其总体健康状况良好。
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引用次数: 5
Prevalence of Clinical Mastitis and the Suitability of Gamma Function in Predicting Milk Yield of Mastitic and Non-Mastitic Friesian x Bunaji Dairy Cows in Zaria, Nigeria 尼日利亚扎里亚乳糜和非乳糜Friesian x Bunaji奶牛临床乳腺炎患病率及Gamma函数预测产奶量的适用性
Pub Date : 2017-05-01 DOI: 10.9790/2380-10050197102
N. H. Moru, J. Umoh, B. Maikai, P. Barje, I. Adeyinka
Bovine mastitis has been known to cause considerable losses in the dairy industry. Knowledge of the occurrence and incidence of clinical mastitis in dairy cows is of great importance for monitoring the health status of the mammary glands, also in institution and implementation of control programs for the disease. The aim of this study was to determine the occurrence of clinical mastitis in Friesian X Bunaji crossbred dairy cows in a Research farm in Zaria, Nigeria. This was a cross-sectional study which involved the use of passive data (lactation records) generated from 2000 to 2015 in the Dairy Research Programme of a National Animal Production Research Institute, Shika-Zaria. The prevalence of clinical mastitis was determined and the lactation curve for the Friesian x Bunaji cows was designed and analyzed using the Gamma Function by Wood(1967). The prevalence of clinical mastitis was found to increase with increasing parity number (0.30, 0.32 and 0.43 for parity 1, 2 and 3 respectively). High prevalence of clinical mastitis occurred in early lactation period of parity 1 cows, but highest prevalence was recorded during the late lactation period in parities 2 and 3 cows.
众所周知,牛乳腺炎会给乳制品行业造成相当大的损失。了解奶牛临床乳腺炎的发生和发病率对于监测乳腺的健康状况,以及制定和实施疾病控制方案具有重要意义。本研究的目的是确定在尼日利亚Zaria研究农场的Friesian X Bunaji杂交奶牛临床乳腺炎的发生情况。这是一项横断面研究,涉及使用2000年至2015年在Shika-Zaria国家动物生产研究所乳制品研究计划中产生的被动数据(泌乳记录)。测定乳腺炎的临床患病率,设计弗里斯和布纳吉奶牛的泌乳曲线,并使用Wood(1967)的Gamma函数进行分析。临床乳腺炎的患病率随着胎次的增加而增加(胎次1、2和3分别为0.30、0.32和0.43)。临床乳腺炎的高发发生在第1胎奶牛的泌乳早期,但最高的发生率发生在第2胎和第3胎奶牛的泌乳后期。
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引用次数: 0
Genetic Diversity, Heritability and Correlation Studies for Yield and its Components in Bread Wheat under Heat Stress Conditions 热胁迫条件下面包小麦产量及其组成因素的遗传多样性、遗传力及相关研究
Pub Date : 2017-05-01 DOI: 10.9790/2380-1005017177
A. U. Islam, A. Chhabra, S. S. Dhanda, O. Peerzada
One hundred diverse bread wheat genotypes were studied for various morpho-physiological traits under timely sown and late sown conditions. The experiment were conducted during the cropping seasons 201213 and 2013-14 at research area of Wheat and Barley Section, Department of Genetics and Plant Breeding, CCSHAU, Hisar. Yield and yield contributing traits were found significant under both conditions. The estimates of GCV, PCV, heritability and genetic advance were high to moderate in timely sown and late sown conditions for the traits yield per plant, number of productive tillers, 1000 grain weight, spike length, number of spikelet per spike, flag leaf area, biological yield, cell membrane stability and grain filling rate. Correlation coefficients revealed that number of productive tillers, 1000 grain weight, number of grains per spike, biological yield, flag leaf area, cell membrane stability, grain filling duration and grain filling rate gave the positive associations with grain yield in both environments. Consequently, selection can be based on these traits in both environments for breeding of bread wheat under heat stress condition.
对100个不同面包小麦基因型在播时和晚播条件下的形态生理性状进行了研究。试验于2012 - 213和2013-14种植季在希萨尔市中央农业大学遗传与植物育种系小麦大麦科研究区进行。在两个条件下,产量和产量贡献性状均显著。单株产量、有效分蘖数、千粒重、穗长、穗粒数、旗叶面积、生物产量、细胞膜稳定性和籽粒灌浆率的GCV、PCV、遗传力和遗传进步在播时和播后条件下均为高至中等水平。相关系数显示,两种环境下,有效分蘖数、千粒重、穗粒数、生物产量、旗叶面积、细胞膜稳定性、籽粒灌浆期和籽粒灌浆速率与籽粒产量呈正相关。因此,在热胁迫条件下,可以根据这些性状在两种环境下选育面包小麦。
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引用次数: 3
Molecular Divergence of Staphylococcus Aureus Isolated from Pork Byproducts Based on Coagulase Gene Polymorphism and The Presence of Enterotoxigenic genes 基于凝固酶基因多态性和产肠毒素基因存在的猪肉副产品金黄色葡萄球菌分子分化
Pub Date : 2017-05-01 DOI: 10.9790/2380-1005013439
A. Hakim, A. Samy, Sohad M. Dorgham, S. Omara, E. Elgabry
Background: Pork by-products are appeared to be a source of consumer coagulase positive S. aureus strains food poisoning. Material and Methods: The genetic lineage of coagulase positive Staphylococcus aureus (coa) recovered from 20 imported and 30 locally manufactured pork byproducts in Cairo was checked using PCR, and restriction fragment length polymorphism analysis (RFLP). Results: A total number of 27 S. aureus were isolated (54%), the coa gene was identified in 17 out of that isolates (62.9%) with molecular sizes ranging from 195 bp to 1196bp. The coa RFLP analysis using BsuRI revealed 4 different band patterns related to the source of isolates. The detection of 7 enterotoxigenic strains out of the 27 S. aureus isolates suggested the public health importance. Conclusion: There were different polymorphism patterns between local and imported isolates with the detection of enterotoxigenic genes represent a public health concern.
背景:猪肉副产品似乎是消费者凝固酶阳性金黄色葡萄球菌菌株食物中毒的来源。材料与方法:采用PCR和限制性片段长度多态性分析(RFLP)对开罗20个进口猪肉和30个本地猪肉副产品中分离到的凝固酶阳性金黄色葡萄球菌(coa)进行遗传谱检测。结果:共分离到27株金黄色葡萄球菌(54%),其中17株(62.9%)分离到coa基因,分子量在195 ~ 1196bp之间。利用BsuRI进行coa RFLP分析,发现与分离株来源相关的4种不同波段。从27株金黄色葡萄球菌分离株中检出7株产肠毒素菌株,提示其对公共卫生的重要性。结论:本地分离株与输入分离株存在不同的多态性模式,产肠毒素基因的检测具有公共卫生意义。
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引用次数: 0
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IOSR Journal of Agriculture and Veterinary Science
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