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THE USE OF TEST-DAY MILK YIELD AND LAMBS WEIGHT FOR PREDICTION OF SOME PRODUCTIVE TRAITS IN AWASSI SHEEP 用试乳日产奶量和羔羊体重预测阿瓦西羊的某些生产性状
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-08-29 DOI: 10.36103/ijas.v54i4.1795
A. MuthannaF., Al-Juwari Lecturer Dept, Anim, Prod., Coll, Agric, Forestry
This study was included 155 records of Awassi ewes and 199 records of lambs born during two seasons (2011-2012), belonging to the College of Agriculture and Forestry, Mosul University. The effect of year was highly significant on all traits except for weight gain from weaning to 180 days (GW180d). Age of dam was significantly affected daily milk yield (DMY), birth weight (BW), weaning weight (WW) and gain from birth to weaning (GBW). Type of birth had significant  effect on DMY, BW,WW and weight at 180 days. The effect of lamb sex on WW, GBW was significant. Significant correlations coefficient were observed between DMY and total milk yield (TMY), months weight of lambs, GBW and GB180d being ranged from 0.46 to 0.99 and -0.44 to 0.99 respectively. The R2 values of prediction equations were high and ranged between 0.81 to 0.99 to predict TMY, and 0.97 to predict lamb weight at 180 days.
本研究纳入摩苏尔大学农林学院两个季节(2011-2012)出生的155只Awassi母羊和199只羔羊记录。除断奶至180d增重(GW180d)外,年份对其他性状的影响均极显著。犊牛日产奶量(DMY)、初生重(BW)、断奶重(WW)和初生至断奶增重(GBW)受日龄影响显著。出生类型对180 d DMY、BW、WW和体重有显著影响。羔羊性别对WW、GBW的影响显著。DMY与总产奶量(TMY)、羔羊月重、GBW和GB180d的相关系数分别为- 0.46 ~ 0.99和-0.44 ~ 0.99。预测公式的R2值较高,预测TMY的R2值在0.81 ~ 0.99之间,预测180日龄羔羊体重的R2值在0.97之间。
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引用次数: 0
EXTENDING THE SHELF-LIFE OF WHEAT BREAD USING PENTOSANES EXTRACTED FROM BARLEY 利用大麦中提取的戊烷延长小麦面包的保质期
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-08-29 DOI: 10.36103/ijas.v54i4.1783
R. G. Nashmi, J. M. Naser, Reasercher Assist. Prof, Dept, Coll. Agric. Engin. Sci Food Sci.
This research was aimed to delay the staling of wheat bread as one of the most used products globally by adding barley pentosanes, which is neglected and not taken into consideration despite their unique and multiple functional properties and their optimum storage temperature. Water-soluble pentosanes (WSP) and water-insoluble pentosanes (WIP)was extracted from barley (Hordeum vulgare) and added to wheat flour at different percentages. Five treatments  were prepared: (T1) from flour only, (T2 and T3) adding WSP at levels  of (1 and 2%), (T4 and T5) adding WIP at the same levels respectively. All treatments were stored at (20, 4 and -18 °C), for 72 h. The results indicated that the addition of pentosans (WSP and WIP) delayed the staling  of bread, especially at the level of addition (2%) for both, when was stored at freezing  temperature (-18°C). The results of the control treatment (T1) and the treatments that included the highest addition level (2%) ( T3 and T5) and after 72 hours of storage at (-18°C) were as follows: The moisture content of bread crumbs decreased to (39.44, 43.32 and 43.91%) respectively. While the moisture in the bread crust increased to (31.61, 33.98, 34.28%), respectively. The swelling power of the bread crumbs and the sediment volume also decreased to (0.74, 1.18, and 1.03%) and (23, 32 and 33%), respectively, which indicates the potency of pentosans and freezing temperature to delay the staling of wheat bread during the storage period.
小麦面包是世界上使用最广泛的食品之一,本研究旨在通过添加大麦戊烷来延缓小麦面包的变质,尽管大麦戊烷具有独特的多种功能特性和最佳储存温度,但它却被忽视和忽视。从大麦(Hordeum vulgare)中提取水溶性戊烷(WSP)和水不溶性戊烷(WIP),并按不同比例添加到小麦粉中。5个处理分别为:(T1)纯面粉处理,(T2和T3)分别添加1%和2%的WSP处理,(T4和T5)分别添加相同水平的WIP处理。结果表明,在冷冻温度(-18°C)下,添加戊聚糖(WSP和WIP)延缓了面包的变质,特别是在添加量(2%)时,这两种处理在冷冻温度(-18°C)下储存。对照处理(T1)和添加量最高(2%)的处理(T3和T5)以及(-18℃)贮藏72 h后,面包屑含水率分别降至(39.44%)、(43.32%)和(43.91%)。面包皮含水率分别为31.61%、33.98、34.28%。面包屑膨胀率和沉淀体积分别降低至0.74%、1.18%和1.03%和23%、32%和33%,说明戊聚糖和冷冻温度对小麦面包贮藏期的延缓变质有一定作用。
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引用次数: 0
EFFECT OF ADDING RESVERATROL TO SOYBEAN-LECITHIN EXTENDER ON SOME SEMEN ATTRIBUTES OF BUFFALO BULLS 大豆卵磷脂扩展剂中添加白藜芦醇对水牛精液某些性状的影响
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-08-29 DOI: 10.36103/ijas.v54i4.1797
A. M. Alhelal, T. A. A. R. Prof., Dept, Anim, Coll. Agric. Engin. Sci Prod.
This study was conducted to explain the effect of adding different resveratrol (Res) concentrations to soybean-lecithin extender on some post-cryopreserved (PC) semen characteristics of the Iraqi buffalo bulls for different preservation periods. Eight mature bulls were used, and polled semen was equally divided into four groups within one trial. Three Res concentrations (100, 150, and 200 μM) were added to the soybean-lecithin extender, and comparisons in the response were made with the control group (soybean-lecithin extender, Res-0). The differences among groups in sperm cell individual motility percentages at different preservation periods lacked significance. Greater (P≤0.01) sperm acrosome integrity percentage was noticed for the Res-150 group compared with the C and Res-200 groups at cooling, 48 hrs., and 2 months PC. Lesser (P≤0.01) malondialdehyde concentrations and sperm DNA damage percentage were observed for the Res-150 group as compared with the other groups, 2 months PC. Higher (P≤0.01) total antioxidant activity was shown in Res-150 and Res-200 groups as compared with the control and Res-100 groups, 2 months PC. In conclusion, adding Res to the soybean-lecithin extender enhanced some of the PC semen characteristics of Iraqi buffalo bulls at different preservation periods.
本研究旨在解释在大豆卵磷脂扩展剂中添加不同浓度的白藜芦醇(Res)对不同保存期伊拉克水牛精液某些冻存后性状的影响。使用八头成年公牛,在一次试验中将精液平均分为四组。在大豆-卵磷脂扩展剂中添加100、150和200 μM的三种Res浓度,并与对照组(大豆-卵磷脂扩展剂Res-0)进行反应比较。不同保存期各组间精子细胞个体活力百分比差异不显著。冷却48 h时,Res-150组精子顶体完整性高于C组和Res-200组(P≤0.01)。和2个月的个人电脑。2个月后,Res-150组丙二醛浓度和精子DNA损伤率低于其他组(P≤0.01)。2个月后,Res-150和Res-200组的总抗氧化活性均高于对照组和Res-100组(P≤0.01)。综上所述,在大豆卵磷脂扩展剂中添加Res可提高伊拉克水牛在不同保存时期的部分PC精液特性。
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引用次数: 0
THE INFLUENCE OF GENISTEIN IMPLANTATION ON OFFSPRING SEX RATIOS AND THEIR RELATION TO ESTROGEN LEVELS IN THE BLOOD OF IRAQI CHICKENS 染料木素植入对伊拉克鸡子代性别比的影响及其与血液雌激素水平的关系
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-08-29 DOI: 10.36103/ijas.v54i4.1790
R. A. S. Al-Ghurairi, A. W.Kh, A. Hayani, Y. M. Maaeni, Researcher Prof Scientific Researcher
This study was carried out at the Poultry Research Station / Agricultural Research Department / Ministry of Agriculture, to investigate the effects of implanting genistein (GE) for the period of 1/February/2021 to 16/August/2021. into Iraqi local chickens at various ages on primary (PSF) and secondary (SSF) sex ratios of female, fertility (FE), and hatchability (HA) traits. At the age of 12 weeks, 100 hens and 20 roosters of Iraqi local chickens from the Poultry Research Station were used in this study. After numbering the hens, the birds were housed in individual cages and divided into four treatments (each with 25 chickens) as follows: T1: none implantation; T2, T3, and T4: implantation of 10 mg GE /kg weight at 14, 18, and 22 weeks of age, respectively. The experiment was divided into three periods, each for 28 weeks, and then rated according to the overall average and all of the traits studied. The results showed that implanting GE into hens had a positive influence on FE, PSF, SSF, and estrogen level (ES), especially at 18 weeks of age. There were also significant correlations between traits and ES in hens’ blood. It was also shown that the regression of most traits on ES is first order linear. As a result, it can be concluded that GE has a positive effect on ES, PSF, SSF, with the possibility of predicting sex ratios and sex offspring based on estrogen levels in the blood, and that implantation at 18 weeks of age has produced great results.
本研究于2021年2月1日至2021年8月16日在农业部家禽研究站/农业研究司/家禽研究站进行,目的是研究植入染料木素(GE)的效果。对不同年龄伊拉克地方鸡的主要(PSF)和次要(SSF)雌性性别比、育性(FE)和孵化率(HA)性状进行研究。试验选用12周龄的伊拉克地方鸡100只母鸡和20只公鸡。母鸡编号后,分笼饲养,每笼25只,分为4个处理:T1:不着床;T2、T3、T4:分别在14、18、22周龄注入10 mg GE /kg体重。实验分为三个阶段,每个阶段28周,然后根据总体平均值和所有研究的特征进行评分。结果表明,饲粮中添加GE对蛋鸡FE、PSF、SSF和雌激素水平(ES)有显著影响,尤其是在18周龄时。母鸡血液中ES与性状也有显著相关。结果表明,大多数性状对ES的回归是一阶线性的。由此可见,GE对ES、PSF、SSF均有正向影响,并有可能根据血液雌激素水平预测性别比和性别后代,且18周龄植入效果显著。
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引用次数: 0
A COMPARATIVE HORMONAL STUDY FOR TWO TYPES OF AMNIOTIC ANIMALS 两种羊膜动物激素的比较研究
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-08-29 DOI: 10.36103/ijas.v54i4.1796
Assist. Prof, A. Lina, Salih
This research aims to study thyroid hormones, growth hormone, and estrogen hormone in two types of Iraqi amniotes. The study included two types of Mature animals, the local rabbit (Oryctolagus cuniculus), an example of mammals, and the Japanese quail (Coturnix coturnix Japanese), an example of birds. Twenty females were taken from each of the rabbits and quails, and they were of sexual maturity and weighed(1347.2 ± 404.2) g and (173.5 ± 42)g respectively. The level of thyroid function T3, T4, TSH, growth hormones (GH), and estrogen (E2) was measured in the blood serum of quail and local rabbit using ELISA technique following the steps in the leflet kit supplied by the company MY BioSource and CUSABIO. The results of the current study.showed a significant increase in the concentration of T3, T4, TSH, and estrogen hormones in the rabbit compared to the quail at a probability of (p≤0.01). The study also showed a Highly significant increase of growth hormone in rabbit serum compared with the quail at probability of (p≤0.05).
本研究旨在研究两种伊拉克羊膜的甲状腺激素、生长激素和雌激素激素。这项研究包括两种成熟的动物,一种是哺乳动物中的本地兔(Oryctolagus cuniculus),另一种是鸟类中的日本鹌鹑(Coturnix Coturnix Japanese)。取性成熟雌兔和鹌鹑各20只,体重分别为(1347.2±404.2)g和(173.5±42)g。按照MY BioSource和CUSABIO公司提供的试剂盒步骤,采用ELISA技术检测鹌鹑和当地家兔血清中甲状腺功能T3、T4、TSH、生长激素(GH)和雌激素(E2)水平。当前研究的结果。与鹌鹑相比,家兔体内T3、T4、TSH和雌激素浓度显著升高,且概率为(p≤0.01)。与鹌鹑相比,家兔血清中生长激素含量显著升高(p≤0.05)。
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引用次数: 0
CYTOTOXIC ACTIVITY OF BASIL SEEDS( Ocimum baslicum L) EXTRACTS ON SOME BREAST CANCER CELL LINES ( IN VITRO) 罗勒种子(ocum baslicum L)提取物对乳腺癌细胞株的细胞毒活性(体外)
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-08-29 DOI: 10.36103/ijas.v54i4.1782
J. M. Awda, Ayeda M.M Researcher Prof, Dept, Food Scine, Coll, Agric, Engin, Sci.
In this study, four types of basil seed extracts were used, being cold aqueous extract, hot aqueous extract, petroleum ether extract, and methanolic extract.Nine concentrations (10000, 5000, 2500, 1250, 625, 312.56, 156.25, 78.125, 39.0, 0.0) µg/ml were used to study the extracts cytotoxicity against two breast cancer cell lines MCF7 and AMJ13 beside REF cell line as normal cells. The methanol extract showed the highest inhibition rate of 64.4% on MCF7 cell line at concentration of 1250 µg/ml and 42.4% on AMJ13 cell line at a concentration  of 39.0 µg/ml with significant difference as compared to  control  sample during the 72hr exposure period. While the petroleum ether extract showed a lower inhibition rate of 16.02% at a concentration of 78.1 µg/ml on MCF7 cell line  and 35.3 % at a concentration of 312.5 µg /ml on AMJ13 cells during a 72hr exposure period, compared with the two aqueous extracts which showed a slight effect compared to the control. The cold aqueous extract showed the slight effect of 24.4% at a concentration of 5000 µg/ml on AMJ13 cell line and no toxicity was shown on MCF7 cells. All extracts showed no effect on REF normal cells. In all effective concentrations the basil seed extracts caused a damage to the DNA that cannot be repaired, and thus the cells are heading to programmed death. This effect on the genetic material was studied using the comet assay and mitotic index.
本研究采用四种罗勒种子提取液,分别为冷水提取液、热水提取液、石油醚提取液和甲醇提取液。以9个浓度(10000、5000、2500、1250、625、312.56、156.25、78.125、39.0、0.0)µg/ml研究其对乳腺癌MCF7和AMJ13的细胞毒性。甲醇提取物在1250µg/ml浓度下对MCF7细胞株的抑制率最高,为64.4%;在39.0µg/ml浓度下对AMJ13细胞株的抑制率为42.4%,与对照样品在72h内差异显著。石油醚提取物在浓度为78.1µg/ml时对MCF7细胞的抑制率为16.02%,在浓度为312.5µg/ml时对AMJ13细胞的抑制率为35.3%。冷水提取物在5000µg/ml浓度下对AMJ13细胞株的影响为24.4%,对MCF7细胞无毒性。所有提取物对REF正常细胞均无影响。在所有有效浓度下,罗勒籽提取物都会对DNA造成无法修复的损伤,因此细胞正在走向程序性死亡。利用彗星试验和有丝分裂指数研究了这种对遗传物质的影响。
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引用次数: 0
THE RELATIONSHIP OF PLASMA SOMATOMEDIN (IGF-1) WITH PERFORMANCE AND CARCASS CHARACTERISTICS OF AWASSI AND KARADI LAMBS 血浆生长激素(igf-1)与阿瓦西和卡拉迪羔羊生产性能和胴体特性的关系
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-08-29 DOI: 10.36103/ijas.v54i4.1803
K. Oray, Dept. Animal Production
Lambs’ performance and some carcass traits in relation to insulin-like growth factor-1 (IGF-1) were studied in twelve lambs from each of Awassi and Karadi slaughtered at three different weights raised at the animal farm, Department of Animal Production, College of Agricultural Engineering Science, University of Duhok.  Each group of lambs slaughtered at 30 kg, 40 kg, and 50kg was kept in an individual separate pen and fed concentrate ration at libitum. Weight gain and feed intake were recorded till the slaughter and blood samples were taken at the beginning of the experiment and one day before slaughter from the vena jugular for the IGF-1 analysis by using the enzyme immunoassay (EIA) method. Statistical analysis showed that the concentrations of insulin-like growth factor (IGF-1) were not affected significantly by breed.  Furthermore, lambs slaughtered at 50 kg, showed numerically the highest IGF-1 concentration, compared to lambs slaughtered at 30 kg and 40kg. There was a significant positive correlation between IGF-1 concentrations and each of the average daily gain and daily feed intake of Awassi lambs slaughtered at 30. Additionally, no consistent results were found between the IGF-1 concentration and carcass composition in studied breeds.
在杜霍克大学农业工程科学学院动物养殖场饲养的阿瓦西羊和卡拉迪羊各12只,以3种不同体重屠宰,研究了羔羊的生产性能和与胰岛素样生长因子-1 (IGF-1)有关的一些胴体性状。30 kg、40 kg和50kg时屠宰的每组羔羊单独饲养,自由饲喂精料日粮。记录增重和采食量直至屠宰,并在试验开始时和屠宰前1天取颈静脉血样,采用酶免疫分析法(EIA)检测IGF-1。统计分析表明,不同品种对胰岛素样生长因子(IGF-1)浓度影响不显著。此外,与30和40公斤屠宰的羔羊相比,50公斤屠宰的羔羊在数值上显示出最高的IGF-1浓度。30岁屠宰阿瓦西羔羊的平均日增重和日采食量均与IGF-1浓度呈显著正相关。此外,在所研究的品种中,IGF-1浓度与胴体组成之间没有一致的结果。
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引用次数: 0
HISTOPATHOLOGICAL CHANGES OF USING RAW LENTIL SEEDS AS A PARTIAL SUBSTITUTE FOR THE FISH MEAL IN DIETS OF THE COMMON CARP CYPRINUS CARPIO L. 生扁豆籽部分替代鲤鱼饲料中鱼粉的组织病理学变化。
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-08-29 DOI: 10.36103/ijas.v54i4.1807
N. M. Abdulrahman, Z. S. Abdulla, S. Prof.
 This experiment was carried out at College of Veterinary Medicine, University of Sulaimani. Microscopically, no adverse histopathological changes were seen in the kidneys, liver, gills and intestines of Cyprinus carpio for T2(replacing fishmeal with 5% lentil) and T3 (replacing fishmeal with 10% lentil) in comparison with the control (T1). However, various levels of adverse histopathological changes were seen in the T4(replacing fish meal with 15% lentil) and T5 (replacing fishmeal with 20% lentil). In the kidneys, dilation of the Bowman’s spaces was evidently associated with a decrease in the mesangial cellularity of glomerular tuft. In the liver, congestion of the central vein was apparent together with centrilobular infiltration of inflammatory cells. In the gills, hyperplasia of primary and secondary lamellae was evident. In the intestine, vacuolar degeneration of the lining epithelial cells was apparent together with extravasation of RBCs associated with marked infiltration of inflammatory cells in the lamina propria. In conclusion, the partial replacement of the fishmeal in diets of C. carpio by crude lentil seeds was safe at the 5% and 10% levels as indicated by various levels of adverse histopathological effects on the kidneys, liver, gills, and intestine.
本实验在苏莱曼尼大学兽医学院进行。显微镜下观察,T2组(用5%扁豆代替鱼粉)和T3组(用10%扁豆代替鱼粉)鲤的肾脏、肝脏、鳃和肠道与对照组(T1组)相比,未见不良的组织病理学变化。然而,在T4(用15%扁豆代替鱼粉)和T5(用20%扁豆代替鱼粉)中观察到不同程度的不良组织病理学变化。在肾脏中,鲍曼间隙的扩张明显与肾小球束系膜细胞的减少有关。肝脏中央静脉明显充血,伴有小叶中心炎性细胞浸润。在鳃中,初级和次级薄片增生明显。在肠内,上皮细胞的空泡变性很明显,红细胞外渗,固有层有明显的炎症细胞浸润。综上所述,在5%和10%的水平下,粗扁豆种子部分替代鲤鱼饲料中的鱼粉是安全的,对鱼的肾脏、肝脏、鳃和肠道产生了不同程度的不良组织病理学影响。
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引用次数: 0
EFFECT OF TOCOPHEROL, TREHALOSE AND SOIL IMPROVEMENT IN WATER PRODUCTIVITY AND INDUSTRIAL POTATOES UNDER WATER STRESS 水分胁迫下生育酚、海藻糖和土壤改良对马铃薯水分生产力和工业马铃薯的影响
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-08-29 DOI: 10.36103/ijas.v54i4.1787
AbdulKarim H. Sh, Al-rubaie, H. KadhimD., Al-Jubouri, R. Prof, Dept, Landscape Gard. Coll Hortic., Agricu, Eng, Sci.
This study was aimed to investigate the impact of tocopherol, trehalose and soil improvement in water productivity and industrial potatoes (Solanum tuberosum L.), Austin hybrid. The field experiments were carried out during the fall season 2020-2021 and spring season 2021. The experiments were carried out as a factorial experiment within a split plot design. The irrigation interval factor was set every 4, 8 and 12 days for the fall season 2020-2021 and every 4, 7 and 10 days for the spring season 2021 (symbolized by I0, I1 and I2) in the main plot, and the interaction factor between Eco Gel soil improvement and anti-stress spray in the Sub plot with three replications. a Eco Gel was added to the soil at the level of 50 kg ha-1 symbolized by E1 and added to the level of 100 kg ha-1 symbolized by E2 as well as the treatment without adding that Its symbol is E0, and spraying with anti-stress, spraying tocopherol at a concentration of 30 mg L-1, symbolized by T1, and spraying trehalose with a concentration of 30 mmol L-1 and symbolized by T2, in addition to spraying with ordinary water, symbolized by T0, the results showed a significant superiority for the treatment of the triple interaction I1E2T2 in the leaf area, the total chlorophyll concentration, the dry weight of the vegetative and the number of marketable tubers The marketable plant yield, the total marketable yield, the relative water content of leaf, the leaf water potential, and the water productivity for the two seasons respectively,compared to treatment I2E0T0.
本研究旨在探讨生育酚、海藻糖和土壤改良对水生产力和工业马铃薯(Solanum tuberosum L.) Austin杂交品种的影响。田间试验分别在2020-2021年秋季和2021年春季进行。实验采用分割图设计进行析因实验。2020-2021年秋季每4、8、12天灌水间隔因子,2021年春季每4、7、10天灌水间隔因子(用I0、I1、I2表示)在主区设置,在次区设置Eco Gel土壤改良与抗旱喷雾的交互作用因子,共3个重复。Eco凝胶添加到土壤的50公斤是象征E1和添加到100公斤是象征的E2水平以及治疗不增加它的象征是E0和喷洒抗应激,喷洒生育酚的浓度30毫克l - 1,象征着由T1和喷洒海藻糖浓度30更易与l - 1和象征T2,除了与普通水喷洒,象征T0,结果表明:三互作I1E2T2处理在两个季节的叶面积、总叶绿素浓度、营养块茎干重和可售块茎数、可售植株产量、总可售产量、叶片相对含水量、叶片水势和水分生产力均显著优于I2E0T0处理。
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引用次数: 0
ROLE OF VITAMIN C AND E ON GENOTOXICITY, HEMATOLOGICAL AND BIOCHEMICAL INVESTIGATION IN CYPRINUS CARPIO L. FOLLOWING ZINC OXIDE NANOPARTICLES EXPOSURE 氧化锌纳米颗粒暴露后,维生素c和e对鲤鱼遗传毒性、血液学和生化的影响
IF 0.6 Q3 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-06-25 DOI: 10.36103/ijas.v54i3.1752
H. H. Faik, S.A. Mustafa
This study was aimed to evaluate the role of vitamins C and E on genotoxicity, biochemical and hematological indices in common carp, Cyprinus carpio following zinc oxide nanoparticles exposure. Zinc oxide nanoparticles were synthesized (size was < 34nm) and characterized using Fourier Transform Infrared Spectra (FTIR) and X-Ray Diffraction (XRD) analysis. About 120 common carp (weight 20.0-32.0 g) were randomly divided into 12 tanks at rate of 10 fish/tank (two (replicates/treatment); fish were fed diet as follows: Control (C) were fed basal diet; T1 fish were fed basal diet mixed with vitamins C and E (400 mg/kg); T2 and T3 fish were fed basal diet mixed with 10%and 15% ZnONPs respectively; T4 and T5  fish were fed basal diet mixed with ZnONPs 10% and 15% plus vitamins C and E (400 mg/kg dw) respectively. Post 40 d feeding trail, variable changes were registered in blood indices (“Hb content, PCV%, WBCs and RBCs numbers”) in all treated groups compared to C and T1 groups. The highest DNA damage (% tail DNA using Comet assay) was seen in T2 and T3 which asserted highly significant increased (P≤0.01) compared to C and to treated groups. As well as, glutathione peroxidase (GPx) activity exhibited highly significant increase (P≤0.01) in T2 and T3 groups relative to C, T4, T1, T5 respectively. This investigation clearly proved that sub-lethal doses (10 and 15% in diet) of ZnONPs were able to induce an oxidative stress in carp fish as reflected by significantly increase of DNA damage to erythrocytes and “the combination of vitamins C and E was able to alleviate the oxidative stress generated due to exposure to ZnONPs.”
本研究旨在探讨维生素C和E对氧化锌纳米颗粒暴露后鲤鱼遗传毒性、生化和血液学指标的影响。合成了氧化锌纳米颗粒(粒径< 34nm),并利用傅里叶变换红外光谱(FTIR)和x射线衍射(XRD)对其进行了表征。试验选取体重20.0 ~ 32.0 g的鲤鱼约120尾,随机分为12个鱼缸,每鱼缸10尾(2个重复/处理);对照组(C)饲喂基础饲粮;T1鱼饲喂混合维生素C和维生素E (400 mg/kg)的基础饲料;T2和T3分别饲喂添加10%和15% ZnONPs的基础饲料;T4和T5鱼分别饲喂在基础饲粮中添加10%和15%的ZnONPs,外加维生素C和E (400 mg/kg dw)。饲喂40 d后,与C组和T1组相比,所有治疗组的血液指标(“Hb含量、PCV%、白细胞和红细胞数量”)均发生了变化。T2和T3的DNA损伤率最高,与C组和处理组相比显著升高(P≤0.01)。谷胱甘肽过氧化物酶(GPx)活性在T2和T3组分别较C、T4、T1、T5组极显著升高(P≤0.01)。这项研究清楚地证明,亚致死剂量(饮食中10%和15%)的ZnONPs能够诱导鲤鱼的氧化应激,这反映在红细胞DNA损伤的显著增加上,“维生素C和E的组合能够减轻由于暴露于ZnONPs而产生的氧化应激。”
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IRAQI JOURNAL OF AGRICULTURAL SCIENCES
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