Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1752
H. H. Faik, S.A. Mustafa
This study was aimed to evaluate the role of vitamins C and E on genotoxicity, biochemical and hematological indices in common carp, Cyprinus carpio following zinc oxide nanoparticles exposure. Zinc oxide nanoparticles were synthesized (size was < 34nm) and characterized using Fourier Transform Infrared Spectra (FTIR) and X-Ray Diffraction (XRD) analysis. About 120 common carp (weight 20.0-32.0 g) were randomly divided into 12 tanks at rate of 10 fish/tank (two (replicates/treatment); fish were fed diet as follows: Control (C) were fed basal diet; T1 fish were fed basal diet mixed with vitamins C and E (400 mg/kg); T2 and T3 fish were fed basal diet mixed with 10%and 15% ZnONPs respectively; T4 and T5 fish were fed basal diet mixed with ZnONPs 10% and 15% plus vitamins C and E (400 mg/kg dw) respectively. Post 40 d feeding trail, variable changes were registered in blood indices (“Hb content, PCV%, WBCs and RBCs numbers”) in all treated groups compared to C and T1 groups. The highest DNA damage (% tail DNA using Comet assay) was seen in T2 and T3 which asserted highly significant increased (P≤0.01) compared to C and to treated groups. As well as, glutathione peroxidase (GPx) activity exhibited highly significant increase (P≤0.01) in T2 and T3 groups relative to C, T4, T1, T5 respectively. This investigation clearly proved that sub-lethal doses (10 and 15% in diet) of ZnONPs were able to induce an oxidative stress in carp fish as reflected by significantly increase of DNA damage to erythrocytes and “the combination of vitamins C and E was able to alleviate the oxidative stress generated due to exposure to ZnONPs.”
{"title":"ROLE OF VITAMIN C AND E ON GENOTOXICITY, HEMATOLOGICAL AND BIOCHEMICAL INVESTIGATION IN CYPRINUS CARPIO L. FOLLOWING ZINC OXIDE NANOPARTICLES EXPOSURE","authors":"H. H. Faik, S.A. Mustafa","doi":"10.36103/ijas.v54i3.1752","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1752","url":null,"abstract":"This study was aimed to evaluate the role of vitamins C and E on genotoxicity, biochemical and hematological indices in common carp, Cyprinus carpio following zinc oxide nanoparticles exposure. Zinc oxide nanoparticles were synthesized (size was < 34nm) and characterized using Fourier Transform Infrared Spectra (FTIR) and X-Ray Diffraction (XRD) analysis. About 120 common carp (weight 20.0-32.0 g) were randomly divided into 12 tanks at rate of 10 fish/tank (two (replicates/treatment); fish were fed diet as follows: Control (C) were fed basal diet; T1 fish were fed basal diet mixed with vitamins C and E (400 mg/kg); T2 and T3 fish were fed basal diet mixed with 10%and 15% ZnONPs respectively; T4 and T5 fish were fed basal diet mixed with ZnONPs 10% and 15% plus vitamins C and E (400 mg/kg dw) respectively. Post 40 d feeding trail, variable changes were registered in blood indices (“Hb content, PCV%, WBCs and RBCs numbers”) in all treated groups compared to C and T1 groups. The highest DNA damage (% tail DNA using Comet assay) was seen in T2 and T3 which asserted highly significant increased (P≤0.01) compared to C and to treated groups. As well as, glutathione peroxidase (GPx) activity exhibited highly significant increase (P≤0.01) in T2 and T3 groups relative to C, T4, T1, T5 respectively. This investigation clearly proved that sub-lethal doses (10 and 15% in diet) of ZnONPs were able to induce an oxidative stress in carp fish as reflected by significantly increase of DNA damage to erythrocytes and “the combination of vitamins C and E was able to alleviate the oxidative stress generated due to exposure to ZnONPs.”","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"36 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73274795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1751
R. M. S. Al-khalidy, R. A. Aburesha
This study was concentrated for isolation and identification of 60 (35.2%) Proteus mirabilis isolates out of 170 urine samples from patients suffering from urinary tract infection from different hospitals in Baghdad city during a period from September 2020 to January 2021. The isolates were cultivated on selective media and biochemical reactions were used to identify them confirmatory APi 20 E tests. The sixty selected isolates were tested for resistance against four antibiotics. The results shown that there were differences in the antibiotic resistance of isolates. High resistance to nalidixic acid and ampicillin were found among isolates as (75%) and (51%) respectively while the resistance of Proteus mirabilis isolates to amikacin and impenem, were(8.3%). Some important virulence factor to Proteus mirabilis was detected by using molecular techniques include PCR and it was found that only 18 (60%) of isolates gave positive result for rsbA at 467 bp. 27 (90%) of them gave positive result for luxS at 464 bp.
{"title":"MOLECULAR DETECTION OF SOME VIRULENCE GENE IN Proteus Mirabilis ISOLATED FROM URINARY TRACT INFECTION IN IRAQ","authors":"R. M. S. Al-khalidy, R. A. Aburesha","doi":"10.36103/ijas.v54i3.1751","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1751","url":null,"abstract":"This study was concentrated for isolation and identification of 60 (35.2%) Proteus mirabilis isolates out of 170 urine samples from patients suffering from urinary tract infection from different hospitals in Baghdad city during a period from September 2020 to January 2021. The isolates were cultivated on selective media and biochemical reactions were used to identify them confirmatory APi 20 E tests. The sixty selected isolates were tested for resistance against four antibiotics. The results shown that there were differences in the antibiotic resistance of isolates. High resistance to nalidixic acid and ampicillin were found among isolates as (75%) and (51%) respectively while the resistance of Proteus mirabilis isolates to amikacin and impenem, were(8.3%). Some important virulence factor to Proteus mirabilis was detected by using molecular techniques include PCR and it was found that only 18 (60%) of isolates gave positive result for rsbA at 467 bp. 27 (90%) of them gave positive result for luxS at 464 bp.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"9 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79915496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1748
D.W.K.Alsaadoon, F. M. Hassan, W. Mahdi, R. Prof, Asisst. Prof
The present study was performed to assess the seasonal changes in the water quality of Diyala River (Diyala Governorate, Iraq), from five sites along the river during the period from September 2020 to April 2021, by applying the Overall Index of Pollution (OIP). OIP is one of the reliable indicators in evaluating the quality of surface water. To calculate the value of the OIP, twelve parameters were measured in the water samples, which are (water temperature, pH, electrical conductivity, dissolved oxygen, biological oxygen demand, total hardness, calcium, magnesium, total dissolved solids, total alkalinity, and concentrations of dissolved nitrates and phosphates). The results showed spatial and temporal variation of physicochemical parameters. The obtained values of OIP (1.72-2.72) revealed acceptable water quality in some sites to slight pollution in the others sites. The current study recommends establishing a novel program for the maintenance and control of Iraqi waters. Such program should be based on scientific means to handle changes that occurred during the past years, such as insufficient release of Iraq’s water shares by neighboring countries, low-rate precipitation rates, and practices of water abuse committed by the population.
{"title":"ASSESSEMENT OF WATER QUALITY OF DIYALA RIVER USING OVERALL INDEX OF POLLUTION (OIP) IN IRAQ","authors":"D.W.K.Alsaadoon, F. M. Hassan, W. Mahdi, R. Prof, Asisst. Prof","doi":"10.36103/ijas.v54i3.1748","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1748","url":null,"abstract":"The present study was performed to assess the seasonal changes in the water quality of Diyala River (Diyala Governorate, Iraq), from five sites along the river during the period from September 2020 to April 2021, by applying the Overall Index of Pollution (OIP). OIP is one of the reliable indicators in evaluating the quality of surface water. To calculate the value of the OIP, twelve parameters were measured in the water samples, which are (water temperature, pH, electrical conductivity, dissolved oxygen, biological oxygen demand, total hardness, calcium, magnesium, total dissolved solids, total alkalinity, and concentrations of dissolved nitrates and phosphates). The results showed spatial and temporal variation of physicochemical parameters. The obtained values of OIP (1.72-2.72) revealed acceptable water quality in some sites to slight pollution in the others sites. The current study recommends establishing a novel program for the maintenance and control of Iraqi waters. Such program should be based on scientific means to handle changes that occurred during the past years, such as insufficient release of Iraq’s water shares by neighboring countries, low-rate precipitation rates, and practices of water abuse committed by the population.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"22 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84249370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1744
A. K. Aljbouri, S. M. Mahmood
This study was conducted to show the effect of Hemazole fungicide on the growth and vitality of earthworms when treated with different concentrations of the pesticide (1000,200,40) mg.kgˉ¹ and for periods of (7,14,21,and28) days, a clear decrease was observed in the growth rate of worms treated with the pesticide with the increase in the time period, and the highest decrease in growth was recorded at the concentration of 40 mg.kgˉ¹ amounted to 83.23% during the third period of rearing (21 days), as for the survival of worms, it was noted that young worms were affected by the three concentrations, and the time period for killing worms 100% was observed after (7, 15 and 21)minutes, respectively, while the time period for the death of adult worms was much higher, reaching (72, 30 and 13) minutes. As for the effect of the pesticide on the thickness of the body wall and the occurrence of tissue abnormalities, it was noted that the thickness of the body wall was significantly affected, especially at the concentration of 40 mg.kgˉ¹, where the lowest thickness was 28,333, 170, 39.67 micrometers compared to the control group, which recorded the thickness of the body wall amounted to 56.667, 532.67, 124 Histological abnormalities were also observed, including necrosis in the epidermal layer, a decrease in the thickness of the longitudinal muscles, and flattening or necrosis in the epithelium of the alimentary canal.
研究了不同浓度(1000、200、40)mg的杀真菌剂Hemazole对蚯蚓生长和活力的影响。在(7、14、21、28)d内，随着时间的增加，虫体的生长速率明显下降，在浓度为40 mg时，虫体的生长速率下降幅度最大。在第3期(21 d)， Kg - h = 83.23%， 3种浓度对幼虫的成虫存活率均有影响，分别在(7、15、21)min后达到100%的杀灭时间，而成虫的杀灭时间要长得多，达到(72、30、13)min。至于农药对体壁厚度和组织异常发生的影响，注意到体壁厚度受到明显影响，特别是在浓度为40 mg时。对照组体壁厚度分别为56.667、532.67、124，体壁厚度分别为28,333、170、39.67微米，并观察到表皮层坏死、纵肌厚度减少、消化道上皮变平或坏死等组织学异常。
{"title":"EVALUTION THE EFFECT OF HEMAZOLE PESTICIDE ON THE VITALITY, GROWTH AND TISSUE CHANGES OF EARTHWORMS","authors":"A. K. Aljbouri, S. M. Mahmood","doi":"10.36103/ijas.v54i3.1744","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1744","url":null,"abstract":"This study was conducted to show the effect of Hemazole fungicide on the growth and vitality of earthworms when treated with different concentrations of the pesticide (1000,200,40) mg.kgˉ¹ and for periods of (7,14,21,and28) days, a clear decrease was observed in the growth rate of worms treated with the pesticide with the increase in the time period, and the highest decrease in growth was recorded at the concentration of 40 mg.kgˉ¹ amounted to 83.23% during the third period of rearing (21 days), as for the survival of worms, it was noted that young worms were affected by the three concentrations, and the time period for killing worms 100% was observed after (7, 15 and 21)minutes, respectively, while the time period for the death of adult worms was much higher, reaching (72, 30 and 13) minutes. As for the effect of the pesticide on the thickness of the body wall and the occurrence of tissue abnormalities, it was noted that the thickness of the body wall was significantly affected, especially at the concentration of 40 mg.kgˉ¹, where the lowest thickness was 28,333, 170, 39.67 micrometers compared to the control group, which recorded the thickness of the body wall amounted to 56.667, 532.67, 124 Histological abnormalities were also observed, including necrosis in the epidermal layer, a decrease in the thickness of the longitudinal muscles, and flattening or necrosis in the epithelium of the alimentary canal.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"10 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82997957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1768
Dina M. Hameed, Nazik H. Khalil
This research was carried out at Research Station , Department of Horticulture and Landscape Engineering , College of Agricultural Engineering Sciences , University of Baghdad during fall season of 2020, it was aimed to improve flower productivity, quantity and quality of essential oils with increasing the concentration of N, P , K elements in the nutrient solution, and foliar app `lication of Plant growth promoters (PGP) on Rosa Damascene grown on a soilless culture system with coco peat substrate bags. A factorial experimental design was carried out according to (RCBD)with three replication. Results indicated the superiority of a high nitrogen treatment and 1.5 g of PGP (N2S2 ), of the flowers number, dry matter%, flower diameter, oil quantity and all physical and the anatomical characteristics, represented by the diameter of the head diameter of the glandular capillaries. Petals number increased significantly with a high potassium nutrient solution and PGP 1 g (N4S1).The high phosphorous solution, which was not treated with growth stimulator, showed superiority in the length of glandular capillaries.
本研究于2020年秋季在巴格达大学农业工程科学学院园艺与景观工程系研究站进行，旨在通过增加营养液中N、P、K元素的浓度来提高花卉产量、精油的数量和质量。植物生长促进剂(PGP)在可可泥炭基质袋无土栽培系统上的叶面应用。按RCBD进行三次重复的析因实验设计。结果表明，高氮处理和1.5 g PGP (N2S2)处理在花数、干物质%、花径、油量以及以腺体毛细血管头直径为代表的所有物理和解剖特征方面都具有优势。在高钾营养液和PGP 1 g (N4S1)处理下，花瓣数量显著增加。未使用生长刺激剂处理的高磷溶液在腺体毛细血管长度方面表现出优势。
{"title":"HIGH NPK SOLUTION AND PGP IMPROVED EOS PROPERTIES OF ROSE GROWN IN COCOPEAT","authors":"Dina M. Hameed, Nazik H. Khalil","doi":"10.36103/ijas.v54i3.1768","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1768","url":null,"abstract":"This research was carried out at Research Station , Department of Horticulture and Landscape Engineering , College of Agricultural Engineering Sciences , University of Baghdad during fall season of 2020, it was aimed to improve flower productivity, quantity and quality of essential oils with increasing the concentration of N, P , K elements in the nutrient solution, and foliar app `lication of Plant growth promoters (PGP) on Rosa Damascene grown on a soilless culture system with coco peat substrate bags. A factorial experimental design was carried out according to (RCBD)with three replication. Results indicated the superiority of a high nitrogen treatment and 1.5 g of PGP (N2S2 ), of the flowers number, dry matter%, flower diameter, oil quantity and all physical and the anatomical characteristics, represented by the diameter of the head diameter of the glandular capillaries. Petals number increased significantly with a high potassium nutrient solution and PGP 1 g (N4S1).The high phosphorous solution, which was not treated with growth stimulator, showed superiority in the length of glandular capillaries.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"33 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72743542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1742
Mohammed M. Farhan, Dhia S. Hassawi, Nabeel K. Ibraheem
Artichoke (Cynara scolymus L.) is a nutritious vegetable that grown all over the world. It is a promising herbal plant, rich in bioactive components. It is considered as medicinal plant due to its nutritional and phytochemical composition, especially high proportion of phenolic compounds. The primary aim of this study was to achieve chemical profile analyses of artichoke for different phytochemcials, especially Scolymoside and Cynaroside. Methanolic crude was extracted from Artichoke leaves by rotary evaporator and separated by column chromatography. The fractions monitored by Thin Layer Chromatography (TLC), and identified in High-Pressure Liquid Chromatography (HPLC). Two important compounds, Scolymoside and Cynaroside were separated and identified. In overall, Artichoke could be regarded as a rich source of biologically active compounds and considered as bio-functional with putative antioxidant effects.
{"title":"EXTRACTION, FRACTIONATION, AND SEPERATIONE OF CYNAROSIDE AND SCOLYMOSIDE FROM Cynara scolymus L. GROWN IN IRAQ","authors":"Mohammed M. Farhan, Dhia S. Hassawi, Nabeel K. Ibraheem","doi":"10.36103/ijas.v54i3.1742","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1742","url":null,"abstract":"Artichoke (Cynara scolymus L.) is a nutritious vegetable that grown all over the world. It is a promising herbal plant, rich in bioactive components. It is considered as medicinal plant due to its nutritional and phytochemical composition, especially high proportion of phenolic compounds. The primary aim of this study was to achieve chemical profile analyses of artichoke for different phytochemcials, especially Scolymoside and Cynaroside. Methanolic crude was extracted from Artichoke leaves by rotary evaporator and separated by column chromatography. The fractions monitored by Thin Layer Chromatography (TLC), and identified in High-Pressure Liquid Chromatography (HPLC). Two important compounds, Scolymoside and Cynaroside were separated and identified. In overall, Artichoke could be regarded as a rich source of biologically active compounds and considered as bio-functional with putative antioxidant effects. ","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"245 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76323063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1756
Maher. S. Al – Rubaye, Atheer. A. Al-doori
Two hundred and fifty (250) samples from both diarrheic and non-diarrheic dogs in both sex male and female multi breeds ( German shepherd and malinois ) in Academy for training police and military dogs for searching about drugs , explosives ,local dogs and pet dogs from different clinical cases in Baghdad city were tested. All samples are obtained in aseptically rectal swabs and transporting to the microbiology lab, for culturing and identification for salmonella species. The study gave 16 (6.4%) positive Salmonella. Virulent gene study gave us 16(6.4%) invA gene, invF 13(5.2%) and sit C 16 (6.4%).Antibiotic sensitivity test According to Bauer-Kirby method were done and gave 16 (6.4%) isolates resistance for ciprofloxacin which is the most clinical antibiotic drug of choice against Salmonella infection . Detection of gyrA gene resistance gene for ciprofloxacin using Polymerase chain reaction (PCR) gave 16 (6.4%) for all positive isolates.
{"title":"DETECTION OF SOME VIRULENCE, ANTIBACTERIAL RESISTANCE GENES FOR SALMONELLA ISOLATED FROM DOGS IN BAGHDAD CITY","authors":"Maher. S. Al – Rubaye, Atheer. A. Al-doori","doi":"10.36103/ijas.v54i3.1756","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1756","url":null,"abstract":"Two hundred and fifty (250) samples from both diarrheic and non-diarrheic dogs in both sex male and female multi breeds ( German shepherd and malinois ) in Academy for training police and military dogs for searching about drugs , explosives ,local dogs and pet dogs from different clinical cases in Baghdad city were tested. All samples are obtained in aseptically rectal swabs and transporting to the microbiology lab, for culturing and identification for salmonella species. The study gave 16 (6.4%) positive Salmonella. Virulent gene study gave us 16(6.4%) invA gene, invF 13(5.2%) and sit C 16 (6.4%).Antibiotic sensitivity test According to Bauer-Kirby method were done and gave 16 (6.4%) isolates resistance for ciprofloxacin which is the most clinical antibiotic drug of choice against Salmonella infection . Detection of gyrA gene resistance gene for ciprofloxacin using Polymerase chain reaction (PCR) gave 16 (6.4%) for all positive isolates.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"45 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85933380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1760
Yousef T. A., M. S. Al-Khshali
This study was aimed of determining the relationship of polymorphism of the growth hormone receptor gene with some of growth traits (daily growth rate, relative and specific growth, ratio and efficiency of feed conversion) in 45 samples of common carp (Cyprinus carpio). The following are the most important results obtained: The results of DNA sequencing and single nucleotide polymorphism (SNP) showed there are of three mutations in the following sites C24074T, A24375G and G24485A. No genetic makeup was significantly associated with the studied growth characteristics. It is concluded from this study that there is no significant effect of the difference in the genotypes of the growth hormone receptor gene on the growth characteristics of common carp fish.
{"title":"RELATIONSHIP OF GROWTH HORMONE RECEPTOR GENE WITH SOME OF PRODUCTIVE TRAITS OF COMMON CARP Cyprinus carpio","authors":"Yousef T. A., M. S. Al-Khshali","doi":"10.36103/ijas.v54i3.1760","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1760","url":null,"abstract":"This study was aimed of determining the relationship of polymorphism of the growth hormone receptor gene with some of growth traits (daily growth rate, relative and specific growth, ratio and efficiency of feed conversion) in 45 samples of common carp (Cyprinus carpio). The following are the most important results obtained: The results of DNA sequencing and single nucleotide polymorphism (SNP) showed there are of three mutations in the following sites C24074T, A24375G and G24485A. No genetic makeup was significantly associated with the studied growth characteristics. It is concluded from this study that there is no significant effect of the difference in the genotypes of the growth hormone receptor gene on the growth characteristics of common carp fish.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"50 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74375103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1740
A.A. H.Yassin, A. A. Al-Kazaz, A. M. Rahmah, T. Y. Ibrahim
This study was aimed to assess expression of IL-22 gene in patients with newly diagnosed autoantibodies against the thyroid antigens; anti-thyroglobulin (anti-TG), and anti-thyroid peroxidase (anti-TPO) in comparison with patients previously diagnosed and treated for autoimmune hypothyroidism as well as healthy control individuals. Twenty-eight patients with positive anti-TG and/or anti-TPO were enrolled in this study along with twelve age- and gender-matched healthy controls. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to assess gene expression and fold change among the study groups. Results revealed anti-TG concentration ranges of 134.3 to 2998.9 IU/mL and anti-TPO concentration ranges of 41.6 to 1018.4 IU/mL in patients while control group individuals were negative for these autoantibodies. The mean fold change of IL-22 expression (2-∆∆Ct) was the highest in newly diagnosed patients compared to patients on treatment for autoimmune hypothyroidism and healthy controls indicating the potential role of IL-22 in the early stages of the disease and its contribution in immune imbalance.
{"title":"STUDY THE EXPRESSION OF IL-22 GENE IN AUTOIMMUNE HYPOTHYROIDISM IN IRAQ","authors":"A.A. H.Yassin, A. A. Al-Kazaz, A. M. Rahmah, T. Y. Ibrahim","doi":"10.36103/ijas.v54i3.1740","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1740","url":null,"abstract":"This study was aimed to assess expression of IL-22 gene in patients with newly diagnosed autoantibodies against the thyroid antigens; anti-thyroglobulin (anti-TG), and anti-thyroid peroxidase (anti-TPO) in comparison with patients previously diagnosed and treated for autoimmune hypothyroidism as well as healthy control individuals. Twenty-eight patients with positive anti-TG and/or anti-TPO were enrolled in this study along with twelve age- and gender-matched healthy controls. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to assess gene expression and fold change among the study groups. Results revealed anti-TG concentration ranges of 134.3 to 2998.9 IU/mL and anti-TPO concentration ranges of 41.6 to 1018.4 IU/mL in patients while control group individuals were negative for these autoantibodies. The mean fold change of IL-22 expression (2-∆∆Ct) was the highest in newly diagnosed patients compared to patients on treatment for autoimmune hypothyroidism and healthy controls indicating the potential role of IL-22 in the early stages of the disease and its contribution in immune imbalance.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"4 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85620981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1757
Mushtaq T. B. Al-Zuhariy
Newcastle disease (ND) is a paramyxovirus-based infectious, highly contagious, and pathogenic avian viral disease. Despite the widespread use of ND vaccinations, ND remains a danger to poultry breeders worldwide. The specific goal of this study was to identify the best vaccination route against ND in the layer hens at production stage following oily vaccine. One hundred chickens at 30 weeks of age were collected from layer flocks (ISSA brown) and randomly divided into four groups. The groups received the following vaccinations: G1: Chicks were vaccinated two doses against ND by (La Sota strain) through drinking water at (30 and 40) weeks. G2: Chicks were vaccinated two doses against ND by (La Sota strain) through cross spray at (30 and 40) weeks. G3: Chicks were vaccinated two doses against ND by (La Sota strain) through intraocular at (30 and 40) weeks. G4: Chicks were not vaccinated and consider as control group. All groups challenge with virulent Newcastle virus isolates in a dose ELD50 105 at 50 weeks. To measure the (IgG, IgA, and IFN-γ) against ND, blood samples were taken at 35, 45, and 55 weeks of age. According to the results of this experiment, the third group, followed by the second group, produced the highest mean (IgG, IgA, and IFN-γ) titres among the vaccinated groups, while the first group produced the lowest titres when compared to the control negative (fourth) group, which recorded the lowest immune response and highly decrease in eggs production. The results were showed that intraocular vaccination with a live vaccine provides layer hens with a higher level of homogenous protection against vvNDV than spraying or drinking water vaccination.
{"title":"EVALUTION OF THE BEST VACCINAL ROUTES AGAINST NEWCASTLE IN THE PRODUCTION STAGE OF LAYING HENS","authors":"Mushtaq T. B. Al-Zuhariy","doi":"10.36103/ijas.v54i3.1757","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1757","url":null,"abstract":"Newcastle disease (ND) is a paramyxovirus-based infectious, highly contagious, and pathogenic avian viral disease. Despite the widespread use of ND vaccinations, ND remains a danger to poultry breeders worldwide. The specific goal of this study was to identify the best vaccination route against ND in the layer hens at production stage following oily vaccine. One hundred chickens at 30 weeks of age were collected from layer flocks (ISSA brown) and randomly divided into four groups. The groups received the following vaccinations: G1: Chicks were vaccinated two doses against ND by (La Sota strain) through drinking water at (30 and 40) weeks. G2: Chicks were vaccinated two doses against ND by (La Sota strain) through cross spray at (30 and 40) weeks. G3: Chicks were vaccinated two doses against ND by (La Sota strain) through intraocular at (30 and 40) weeks. G4: Chicks were not vaccinated and consider as control group. All groups challenge with virulent Newcastle virus isolates in a dose ELD50 105 at 50 weeks. To measure the (IgG, IgA, and IFN-γ) against ND, blood samples were taken at 35, 45, and 55 weeks of age. According to the results of this experiment, the third group, followed by the second group, produced the highest mean (IgG, IgA, and IFN-γ) titres among the vaccinated groups, while the first group produced the lowest titres when compared to the control negative (fourth) group, which recorded the lowest immune response and highly decrease in eggs production. The results were showed that intraocular vaccination with a live vaccine provides layer hens with a higher level of homogenous protection against vvNDV than spraying or drinking water vaccination.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"17 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83400061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：