Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1751
R. M. S. Al-khalidy, R. A. Aburesha
This study was concentrated for isolation and identification of 60 (35.2%) Proteus mirabilis isolates out of 170 urine samples from patients suffering from urinary tract infection from different hospitals in Baghdad city during a period from September 2020 to January 2021. The isolates were cultivated on selective media and biochemical reactions were used to identify them confirmatory APi 20 E tests. The sixty selected isolates were tested for resistance against four antibiotics. The results shown that there were differences in the antibiotic resistance of isolates. High resistance to nalidixic acid and ampicillin were found among isolates as (75%) and (51%) respectively while the resistance of Proteus mirabilis isolates to amikacin and impenem, were(8.3%). Some important virulence factor to Proteus mirabilis was detected by using molecular techniques include PCR and it was found that only 18 (60%) of isolates gave positive result for rsbA at 467 bp. 27 (90%) of them gave positive result for luxS at 464 bp.
{"title":"MOLECULAR DETECTION OF SOME VIRULENCE GENE IN Proteus Mirabilis ISOLATED FROM URINARY TRACT INFECTION IN IRAQ","authors":"R. M. S. Al-khalidy, R. A. Aburesha","doi":"10.36103/ijas.v54i3.1751","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1751","url":null,"abstract":"This study was concentrated for isolation and identification of 60 (35.2%) Proteus mirabilis isolates out of 170 urine samples from patients suffering from urinary tract infection from different hospitals in Baghdad city during a period from September 2020 to January 2021. The isolates were cultivated on selective media and biochemical reactions were used to identify them confirmatory APi 20 E tests. The sixty selected isolates were tested for resistance against four antibiotics. The results shown that there were differences in the antibiotic resistance of isolates. High resistance to nalidixic acid and ampicillin were found among isolates as (75%) and (51%) respectively while the resistance of Proteus mirabilis isolates to amikacin and impenem, were(8.3%). Some important virulence factor to Proteus mirabilis was detected by using molecular techniques include PCR and it was found that only 18 (60%) of isolates gave positive result for rsbA at 467 bp. 27 (90%) of them gave positive result for luxS at 464 bp.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"9 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79915496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1748
D.W.K.Alsaadoon, F. M. Hassan, W. Mahdi, R. Prof, Asisst. Prof
The present study was performed to assess the seasonal changes in the water quality of Diyala River (Diyala Governorate, Iraq), from five sites along the river during the period from September 2020 to April 2021, by applying the Overall Index of Pollution (OIP). OIP is one of the reliable indicators in evaluating the quality of surface water. To calculate the value of the OIP, twelve parameters were measured in the water samples, which are (water temperature, pH, electrical conductivity, dissolved oxygen, biological oxygen demand, total hardness, calcium, magnesium, total dissolved solids, total alkalinity, and concentrations of dissolved nitrates and phosphates). The results showed spatial and temporal variation of physicochemical parameters. The obtained values of OIP (1.72-2.72) revealed acceptable water quality in some sites to slight pollution in the others sites. The current study recommends establishing a novel program for the maintenance and control of Iraqi waters. Such program should be based on scientific means to handle changes that occurred during the past years, such as insufficient release of Iraq’s water shares by neighboring countries, low-rate precipitation rates, and practices of water abuse committed by the population.
{"title":"ASSESSEMENT OF WATER QUALITY OF DIYALA RIVER USING OVERALL INDEX OF POLLUTION (OIP) IN IRAQ","authors":"D.W.K.Alsaadoon, F. M. Hassan, W. Mahdi, R. Prof, Asisst. Prof","doi":"10.36103/ijas.v54i3.1748","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1748","url":null,"abstract":"The present study was performed to assess the seasonal changes in the water quality of Diyala River (Diyala Governorate, Iraq), from five sites along the river during the period from September 2020 to April 2021, by applying the Overall Index of Pollution (OIP). OIP is one of the reliable indicators in evaluating the quality of surface water. To calculate the value of the OIP, twelve parameters were measured in the water samples, which are (water temperature, pH, electrical conductivity, dissolved oxygen, biological oxygen demand, total hardness, calcium, magnesium, total dissolved solids, total alkalinity, and concentrations of dissolved nitrates and phosphates). The results showed spatial and temporal variation of physicochemical parameters. The obtained values of OIP (1.72-2.72) revealed acceptable water quality in some sites to slight pollution in the others sites. The current study recommends establishing a novel program for the maintenance and control of Iraqi waters. Such program should be based on scientific means to handle changes that occurred during the past years, such as insufficient release of Iraq’s water shares by neighboring countries, low-rate precipitation rates, and practices of water abuse committed by the population.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"22 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84249370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1744
A. K. Aljbouri, S. M. Mahmood
This study was conducted to show the effect of Hemazole fungicide on the growth and vitality of earthworms when treated with different concentrations of the pesticide (1000,200,40) mg.kgˉ¹ and for periods of (7,14,21,and28) days, a clear decrease was observed in the growth rate of worms treated with the pesticide with the increase in the time period, and the highest decrease in growth was recorded at the concentration of 40 mg.kgˉ¹ amounted to 83.23% during the third period of rearing (21 days), as for the survival of worms, it was noted that young worms were affected by the three concentrations, and the time period for killing worms 100% was observed after (7, 15 and 21)minutes, respectively, while the time period for the death of adult worms was much higher, reaching (72, 30 and 13) minutes. As for the effect of the pesticide on the thickness of the body wall and the occurrence of tissue abnormalities, it was noted that the thickness of the body wall was significantly affected, especially at the concentration of 40 mg.kgˉ¹, where the lowest thickness was 28,333, 170, 39.67 micrometers compared to the control group, which recorded the thickness of the body wall amounted to 56.667, 532.67, 124 Histological abnormalities were also observed, including necrosis in the epidermal layer, a decrease in the thickness of the longitudinal muscles, and flattening or necrosis in the epithelium of the alimentary canal.
研究了不同浓度(1000、200、40)mg的杀真菌剂Hemazole对蚯蚓生长和活力的影响。在(7、14、21、28)d内，随着时间的增加，虫体的生长速率明显下降，在浓度为40 mg时，虫体的生长速率下降幅度最大。在第3期(21 d)， Kg - h = 83.23%， 3种浓度对幼虫的成虫存活率均有影响，分别在(7、15、21)min后达到100%的杀灭时间，而成虫的杀灭时间要长得多，达到(72、30、13)min。至于农药对体壁厚度和组织异常发生的影响，注意到体壁厚度受到明显影响，特别是在浓度为40 mg时。对照组体壁厚度分别为56.667、532.67、124，体壁厚度分别为28,333、170、39.67微米，并观察到表皮层坏死、纵肌厚度减少、消化道上皮变平或坏死等组织学异常。
{"title":"EVALUTION THE EFFECT OF HEMAZOLE PESTICIDE ON THE VITALITY, GROWTH AND TISSUE CHANGES OF EARTHWORMS","authors":"A. K. Aljbouri, S. M. Mahmood","doi":"10.36103/ijas.v54i3.1744","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1744","url":null,"abstract":"This study was conducted to show the effect of Hemazole fungicide on the growth and vitality of earthworms when treated with different concentrations of the pesticide (1000,200,40) mg.kgˉ¹ and for periods of (7,14,21,and28) days, a clear decrease was observed in the growth rate of worms treated with the pesticide with the increase in the time period, and the highest decrease in growth was recorded at the concentration of 40 mg.kgˉ¹ amounted to 83.23% during the third period of rearing (21 days), as for the survival of worms, it was noted that young worms were affected by the three concentrations, and the time period for killing worms 100% was observed after (7, 15 and 21)minutes, respectively, while the time period for the death of adult worms was much higher, reaching (72, 30 and 13) minutes. As for the effect of the pesticide on the thickness of the body wall and the occurrence of tissue abnormalities, it was noted that the thickness of the body wall was significantly affected, especially at the concentration of 40 mg.kgˉ¹, where the lowest thickness was 28,333, 170, 39.67 micrometers compared to the control group, which recorded the thickness of the body wall amounted to 56.667, 532.67, 124 Histological abnormalities were also observed, including necrosis in the epidermal layer, a decrease in the thickness of the longitudinal muscles, and flattening or necrosis in the epithelium of the alimentary canal.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"10 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82997957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1759
Zahraa K. K. Al- Salihi, Salloom B. Salim
A field experiment was carried out during the spring season 2020 at the University of Baghdad to study the effect of the length and depth of the drip tape on wetting advance ofsubsurface drip irrigation system using Hydrus 2D/3D program. The site was planted with sunflower in a clay loam texture. The drip tape was installed at 0.3m below soil surfacein two lengths; 20m and 40m. The experiment was designed according to the arrangement of split plotsdesign and with three replications. The treatments were randomly distributed and planted with sunflower crop. The results showed that the moisture contents of the 20 mlength drip tape was higher compared with the 40 m drip tape length. Also, the length of the tape was superior in some components of the yield if the length of the drip tape exceeded 20 m in the character of the number of seeds in one disc, and the result was a significant superiority.
{"title":"EFFECT OF LENGTHANDDEPTHOF THEDRIPTAPEON WETTING FRONT ADVANCE IN THE ROOT ZONE OF SUNFLOWR USING HYDRUS 2D/3D PROGRAM","authors":"Zahraa K. K. Al- Salihi, Salloom B. Salim","doi":"10.36103/ijas.v54i3.1759","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1759","url":null,"abstract":"A field experiment was carried out during the spring season 2020 at the University of Baghdad to study the effect of the length and depth of the drip tape on wetting advance ofsubsurface drip irrigation system using Hydrus 2D/3D program. The site was planted with sunflower in a clay loam texture. The drip tape was installed at 0.3m below soil surfacein two lengths; 20m and 40m. The experiment was designed according to the arrangement of split plotsdesign and with three replications. The treatments were randomly distributed and planted with sunflower crop. The results showed that the moisture contents of the 20 mlength drip tape was higher compared with the 40 m drip tape length. Also, the length of the tape was superior in some components of the yield if the length of the drip tape exceeded 20 m in the character of the number of seeds in one disc, and the result was a significant superiority.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"81 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90504106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1768
Dina M. Hameed, Nazik H. Khalil
This research was carried out at Research Station , Department of Horticulture and Landscape Engineering , College of Agricultural Engineering Sciences , University of Baghdad during fall season of 2020, it was aimed to improve flower productivity, quantity and quality of essential oils with increasing the concentration of N, P , K elements in the nutrient solution, and foliar app `lication of Plant growth promoters (PGP) on Rosa Damascene grown on a soilless culture system with coco peat substrate bags. A factorial experimental design was carried out according to (RCBD)with three replication. Results indicated the superiority of a high nitrogen treatment and 1.5 g of PGP (N2S2 ), of the flowers number, dry matter%, flower diameter, oil quantity and all physical and the anatomical characteristics, represented by the diameter of the head diameter of the glandular capillaries. Petals number increased significantly with a high potassium nutrient solution and PGP 1 g (N4S1).The high phosphorous solution, which was not treated with growth stimulator, showed superiority in the length of glandular capillaries.
本研究于2020年秋季在巴格达大学农业工程科学学院园艺与景观工程系研究站进行，旨在通过增加营养液中N、P、K元素的浓度来提高花卉产量、精油的数量和质量。植物生长促进剂(PGP)在可可泥炭基质袋无土栽培系统上的叶面应用。按RCBD进行三次重复的析因实验设计。结果表明，高氮处理和1.5 g PGP (N2S2)处理在花数、干物质%、花径、油量以及以腺体毛细血管头直径为代表的所有物理和解剖特征方面都具有优势。在高钾营养液和PGP 1 g (N4S1)处理下，花瓣数量显著增加。未使用生长刺激剂处理的高磷溶液在腺体毛细血管长度方面表现出优势。
{"title":"HIGH NPK SOLUTION AND PGP IMPROVED EOS PROPERTIES OF ROSE GROWN IN COCOPEAT","authors":"Dina M. Hameed, Nazik H. Khalil","doi":"10.36103/ijas.v54i3.1768","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1768","url":null,"abstract":"This research was carried out at Research Station , Department of Horticulture and Landscape Engineering , College of Agricultural Engineering Sciences , University of Baghdad during fall season of 2020, it was aimed to improve flower productivity, quantity and quality of essential oils with increasing the concentration of N, P , K elements in the nutrient solution, and foliar app `lication of Plant growth promoters (PGP) on Rosa Damascene grown on a soilless culture system with coco peat substrate bags. A factorial experimental design was carried out according to (RCBD)with three replication. Results indicated the superiority of a high nitrogen treatment and 1.5 g of PGP (N2S2 ), of the flowers number, dry matter%, flower diameter, oil quantity and all physical and the anatomical characteristics, represented by the diameter of the head diameter of the glandular capillaries. Petals number increased significantly with a high potassium nutrient solution and PGP 1 g (N4S1).The high phosphorous solution, which was not treated with growth stimulator, showed superiority in the length of glandular capillaries.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"33 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72743542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1742
Mohammed M. Farhan, Dhia S. Hassawi, Nabeel K. Ibraheem
Artichoke (Cynara scolymus L.) is a nutritious vegetable that grown all over the world. It is a promising herbal plant, rich in bioactive components. It is considered as medicinal plant due to its nutritional and phytochemical composition, especially high proportion of phenolic compounds. The primary aim of this study was to achieve chemical profile analyses of artichoke for different phytochemcials, especially Scolymoside and Cynaroside. Methanolic crude was extracted from Artichoke leaves by rotary evaporator and separated by column chromatography. The fractions monitored by Thin Layer Chromatography (TLC), and identified in High-Pressure Liquid Chromatography (HPLC). Two important compounds, Scolymoside and Cynaroside were separated and identified. In overall, Artichoke could be regarded as a rich source of biologically active compounds and considered as bio-functional with putative antioxidant effects.
{"title":"EXTRACTION, FRACTIONATION, AND SEPERATIONE OF CYNAROSIDE AND SCOLYMOSIDE FROM Cynara scolymus L. GROWN IN IRAQ","authors":"Mohammed M. Farhan, Dhia S. Hassawi, Nabeel K. Ibraheem","doi":"10.36103/ijas.v54i3.1742","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1742","url":null,"abstract":"Artichoke (Cynara scolymus L.) is a nutritious vegetable that grown all over the world. It is a promising herbal plant, rich in bioactive components. It is considered as medicinal plant due to its nutritional and phytochemical composition, especially high proportion of phenolic compounds. The primary aim of this study was to achieve chemical profile analyses of artichoke for different phytochemcials, especially Scolymoside and Cynaroside. Methanolic crude was extracted from Artichoke leaves by rotary evaporator and separated by column chromatography. The fractions monitored by Thin Layer Chromatography (TLC), and identified in High-Pressure Liquid Chromatography (HPLC). Two important compounds, Scolymoside and Cynaroside were separated and identified. In overall, Artichoke could be regarded as a rich source of biologically active compounds and considered as bio-functional with putative antioxidant effects. ","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"245 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76323063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1756
Maher. S. Al – Rubaye, Atheer. A. Al-doori
Two hundred and fifty (250) samples from both diarrheic and non-diarrheic dogs in both sex male and female multi breeds ( German shepherd and malinois ) in Academy for training police and military dogs for searching about drugs , explosives ,local dogs and pet dogs from different clinical cases in Baghdad city were tested. All samples are obtained in aseptically rectal swabs and transporting to the microbiology lab, for culturing and identification for salmonella species. The study gave 16 (6.4%) positive Salmonella. Virulent gene study gave us 16(6.4%) invA gene, invF 13(5.2%) and sit C 16 (6.4%).Antibiotic sensitivity test According to Bauer-Kirby method were done and gave 16 (6.4%) isolates resistance for ciprofloxacin which is the most clinical antibiotic drug of choice against Salmonella infection . Detection of gyrA gene resistance gene for ciprofloxacin using Polymerase chain reaction (PCR) gave 16 (6.4%) for all positive isolates.
{"title":"DETECTION OF SOME VIRULENCE, ANTIBACTERIAL RESISTANCE GENES FOR SALMONELLA ISOLATED FROM DOGS IN BAGHDAD CITY","authors":"Maher. S. Al – Rubaye, Atheer. A. Al-doori","doi":"10.36103/ijas.v54i3.1756","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1756","url":null,"abstract":"Two hundred and fifty (250) samples from both diarrheic and non-diarrheic dogs in both sex male and female multi breeds ( German shepherd and malinois ) in Academy for training police and military dogs for searching about drugs , explosives ,local dogs and pet dogs from different clinical cases in Baghdad city were tested. All samples are obtained in aseptically rectal swabs and transporting to the microbiology lab, for culturing and identification for salmonella species. The study gave 16 (6.4%) positive Salmonella. Virulent gene study gave us 16(6.4%) invA gene, invF 13(5.2%) and sit C 16 (6.4%).Antibiotic sensitivity test According to Bauer-Kirby method were done and gave 16 (6.4%) isolates resistance for ciprofloxacin which is the most clinical antibiotic drug of choice against Salmonella infection . Detection of gyrA gene resistance gene for ciprofloxacin using Polymerase chain reaction (PCR) gave 16 (6.4%) for all positive isolates.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"45 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85933380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1760
Yousef T. A., M. S. Al-Khshali
This study was aimed of determining the relationship of polymorphism of the growth hormone receptor gene with some of growth traits (daily growth rate, relative and specific growth, ratio and efficiency of feed conversion) in 45 samples of common carp (Cyprinus carpio). The following are the most important results obtained: The results of DNA sequencing and single nucleotide polymorphism (SNP) showed there are of three mutations in the following sites C24074T, A24375G and G24485A. No genetic makeup was significantly associated with the studied growth characteristics. It is concluded from this study that there is no significant effect of the difference in the genotypes of the growth hormone receptor gene on the growth characteristics of common carp fish.
{"title":"RELATIONSHIP OF GROWTH HORMONE RECEPTOR GENE WITH SOME OF PRODUCTIVE TRAITS OF COMMON CARP Cyprinus carpio","authors":"Yousef T. A., M. S. Al-Khshali","doi":"10.36103/ijas.v54i3.1760","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1760","url":null,"abstract":"This study was aimed of determining the relationship of polymorphism of the growth hormone receptor gene with some of growth traits (daily growth rate, relative and specific growth, ratio and efficiency of feed conversion) in 45 samples of common carp (Cyprinus carpio). The following are the most important results obtained: The results of DNA sequencing and single nucleotide polymorphism (SNP) showed there are of three mutations in the following sites C24074T, A24375G and G24485A. No genetic makeup was significantly associated with the studied growth characteristics. It is concluded from this study that there is no significant effect of the difference in the genotypes of the growth hormone receptor gene on the growth characteristics of common carp fish.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"50 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74375103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1741
R. .. Al-Niemi, A. A. Al-Kshab
Alburnus mossulensis has been exposed to lead and cadmium for (96)h in order to determination lethal concentration 50 (LC50) value. A total of 140 fish used in this experiment divided into two groups each group contain 70 fishes .The first group exposure to pbcl2 while the second group exposure to cdcl2. Method of Finney Probit Analysis used to analysis data. LC50 value for PbCl2 and CdCl2 was (57.8 and 29.6) mg/l respectively. Then used about 200 fishes divided into 4 groups, the first group exposure to 1/2LC50 of PbCl2 ,the second group exposure to 1/2LC50 CdCl2 while the third groups contained (1/2LC50 PbCl2+1/2LC50 of CdCl2) as so as control group for peroids (1,4,7 and 14) days to determination level of antioxidants Glutathione (GSH) and the level of lipid peroxidation (Malondialdehyde MDA) . The testes were carried out as three replications, lead ,cadmium and their mixture induced oxidative stress . The results of this study explained significant decrease of the level Glutathione and significant increase (Tukey test, P˂0.05) of the level of Malondialdehye compared with control in all treatments . It is possible to conclude that lead and Cadmium have cytotoxic effects, Fish mortality rates increase with increasing concentrations used and increasing exposure periods.
{"title":"PHYSIOLOGICAL EFFECTS OF LEAD ,CADMIUM AND THEIR MIXTURE ON SOME ORGANS OF Alburnus Mossulensis FISH","authors":"R. .. Al-Niemi, A. A. Al-Kshab","doi":"10.36103/ijas.v54i3.1741","DOIUrl":"https://doi.org/10.36103/ijas.v54i3.1741","url":null,"abstract":" Alburnus mossulensis has been exposed to lead and cadmium for (96)h in order to determination lethal concentration 50 (LC50) value. A total of 140 fish used in this experiment divided into two groups each group contain 70 fishes .The first group exposure to pbcl2 while the second group exposure to cdcl2. Method of Finney Probit Analysis used to analysis data. LC50 value for PbCl2 and CdCl2 was (57.8 and 29.6) mg/l respectively. Then used about 200 fishes divided into 4 groups, the first group exposure to 1/2LC50 of PbCl2 ,the second group exposure to 1/2LC50 CdCl2 while the third groups contained (1/2LC50 PbCl2+1/2LC50 of CdCl2) as so as control group for peroids (1,4,7 and 14) days to determination level of antioxidants Glutathione (GSH) and the level of lipid peroxidation (Malondialdehyde MDA) . The testes were carried out as three replications, lead ,cadmium and their mixture induced oxidative stress . The results of this study explained significant decrease of the level Glutathione and significant increase (Tukey test, P˂0.05) of the level of Malondialdehye compared with control in all treatments . It is possible to conclude that lead and Cadmium have cytotoxic effects, Fish mortality rates increase with increasing concentrations used and increasing exposure periods.","PeriodicalId":14562,"journal":{"name":"IRAQI JOURNAL OF AGRICULTURAL SCIENCES","volume":"41 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82212977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.36103/ijas.v54i3.1757
Mushtaq T. B. Al-Zuhariy
Newcastle disease (ND) is a paramyxovirus-based infectious, highly contagious, and pathogenic avian viral disease. Despite the widespread use of ND vaccinations, ND remains a danger to poultry breeders worldwide. The specific goal of this study was to identify the best vaccination route against ND in the layer hens at production stage following oily vaccine. One hundred chickens at 30 weeks of age were collected from layer flocks (ISSA brown) and randomly divided into four groups. The groups received the following vaccinations: G1: Chicks were vaccinated two doses against ND by (La Sota strain) through drinking water at (30 and 40) weeks. G2: Chicks were vaccinated two doses against ND by (La Sota strain) through cross spray at (30 and 40) weeks. G3: Chicks were vaccinated two doses against ND by (La Sota strain) through intraocular at (30 and 40) weeks. G4: Chicks were not vaccinated and consider as control group. All groups challenge with virulent Newcastle virus isolates in a dose ELD50 105 at 50 weeks. To measure the (IgG, IgA, and IFN-γ) against ND, blood samples were taken at 35, 45, and 55 weeks of age. According to the results of this experiment, the third group, followed by the second group, produced the highest mean (IgG, IgA, and IFN-γ) titres among the vaccinated groups, while the first group produced the lowest titres when compared to the control negative (fourth) group, which recorded the lowest immune response and highly decrease in eggs production. The results were showed that intraocular vaccination with a live vaccine provides layer hens with a higher level of homogenous protection against vvNDV than spraying or drinking water vaccination.
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