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EFFECT OF ELASTIN HYDROLYSATE ON BACTERIA AND SOME SENSORY TRAITS OF CHILLED GROUND BEEF 弹性蛋白水解物对冷藏碎牛肉的细菌和一些感官特征的影响
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/8w3frt36
G. M. AL-Ghanimi, Alrubeii A. M. S Researcher Prof.
This study was aimed to evaluate the effect of elastin hydrolysates on the microbial content and sensory evaluation of ground beef refrigerated at 2°C for 12 days. The study included 10 treatments: T1 without addition, T2 addition (1000ppm/kg meat) and T3 addition (500ppm/kg meat) of elastin hydrolysates produced by the elastase enzyme (E.H.E).T4 addition (1000ppm/kg meat) and T5 addition (500ppm/kg meat) of Elastin hydrolysates produced by the collagenase enzyme (E.H.C), T6 addition (1000ppm/kg meat) and T7 addition (500ppm/kg meat)of elastin hydrolysates produced by the trypsin enzyme (E.H.T), T8 addition and (1000ppm/kg meat) T9 addition (500ppm/kg meat) of elastin hydrolysate produced by the pepsin enzyme(E.H.P) and T10 added to the antioxidant BHA (0.01) Per kg of meat. The treatments were stored cold for periods of 1, 3, 6, 9 and 12 days, and some laboratory tests were conducted on them that showed the effect of elastin hydrolysates when their effectiveness was tested against Escherichia coli Staphylococcus aureus bacteria. When hydrolysates were added to cold-stored minced beef, the lowest number of bacteria was recorded, as they decreased the logarithm. Total Plate Count TPC37, total psychrophilic count TPC4, and E.coli bacteria. Addition treatments also recorded significant increase in degrees of tenderness, flavor, juiciness, and general acceptability.
本研究旨在评估弹性蛋白水解物对在 2°C 下冷藏 12 天的碎牛肉中微生物含量和感官评价的影响。研究包括 10 个处理:T1 不添加、T2 添加(1000ppm/kg 肉)和 T3 添加(500ppm/kg 肉)由弹性蛋白酶(E.H.E)产生的弹性蛋白水解物、T4 添加(1000ppm/kg 肉)和 T5 添加(500ppm/kg 肉)由胶原酶(E.H.C)产生的弹性蛋白水解物、T6 添加(1000ppm/kg 肉)和 T7 添加(500ppm/kg 肉)由胶原酶(E.H.C)产生的弹性蛋白水解物。C),T6 添加(1000ppm/kg 肉)和 T7 添加(500ppm/kg 肉)胰蛋白酶(E.H.T)产生的弹性蛋白水解物,T8 添加和(1000ppm/kg 肉)T9 添加(500ppm/kg 肉)胃蛋白酶(E.H.P)产生的弹性蛋白水解物,T10 添加抗氧化剂 BHA(0.01)/kg 肉。将处理过的肉冷藏 1、3、6、9 和 12 天,并对它们进行了一些实验室测试,结果表明弹性蛋白水解物对大肠杆菌、金黄色葡萄球菌的抗菌效果很好。将水解物添加到冷藏的碎牛肉中时,记录到的细菌数量最少,因为它们的对数减少了。菌落总数 TPC37、总嗜酸性菌落总数 TPC4 和大肠杆菌。添加处理还显著提高了牛肉的嫩度、风味、多汁性和总体可接受性。
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引用次数: 0
MICROPROPAGATION AND ASSESSMENT OF GENETIC FIDELITY OF REGENERATE BY RAPD MARKERS OF SOLANUM NIGRUM 黑茄科植物的微繁殖和利用 RAPD 标记评估再生植物的遗传保真度
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/tnx6m684
Farqad M. K. ALdabbagh, I. H. M. AlZaidi, Magida Alshamari
In vitro propagation approach has been adopted for micropropagation and conservation of Solanum nigrum (black nightshade) to guarantee its sustainable phyto-industry and research availability. Within 1 month, in vitro shoot differentiated was accomplished by culturing shoot tip, nodal segment, and leaf on MS medium enriched with various BA (Benzyl Adenine) concentrations. A significant difference was observed with shoot tips compared with nodal explants and leaf at the shoot induction percentage and mean number of shoot per explant. The highest percentage of shoot induction (100%) and average number of micro shoot per shoo tip (9.200 shoot) were recorded on MS medium fortified with 1 mg/l BA. The findings showed that a satisfactory rooting percentage (100%) was recorded when I mg/l IBA (Indole Butyric Acid) added to half MS medium with  an average number of roots (2.600) root/shoot, a mean root length is 8.600cm. rooted plantlets could be successfully acclimatized in the shade house with 100% survival rate. The genetic stability of the micropropagated plants investigated by the RAPD markers indicated monomorphic and true to type when compare with the original plant.
黑茄科植物黑茄属(Solanum nigrum)的微繁殖和保护采用了离体繁殖方法,以确保其可持续的植物产业和研究可用性。通过在富含不同浓度 BA(苄基腺嘌呤)的 MS 培养基上培养芽尖、节段和叶片,在 1 个月内完成了离体芽分化。与节段外植体和叶片相比,芽尖的芽诱导率和每个外植体的平均芽数有明显差异。在添加了 1 毫克/升 BA 的 MS 培养基上,芽的诱导率最高(100%),每个芽尖的平均微芽数最高(9.200 个)。研究结果表明,在一半的 MS 培养基中添加 I mg/l IBA(吲哚丁酸)时,生根率(100%)令人满意,平均根数(2.600)根/芽,平均根长 8.600 厘米。通过 RAPD 标记对微繁殖植株的遗传稳定性进行了研究,结果表明,与原植株相比,微繁殖植株具有单形性和真型性。
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引用次数: 0
SOME BIOCHEMICAL ATTRIBUTES IN SEMINAL PLASMA OF IRAQI BUFFALO BULLS AND THEIR RELATION TO THE SEMEN QUALITY 伊拉克水牛精浆中的一些生化属性及其与精液质量的关系
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/nrfkex70
K. S. Musa, T. A. A. R. Prof, Dept, Anim, Coll. Agric. Engin. Sci Prod.
This study was undertaken to figure out the relationship between some biochemical characteristics in seminal plasma with the semen quality of Iraqi buffalo bulls (Bubalus bubalis). Semen was diluted with the Tris extender and divided based on the sperm cell's individual motility into two groups. The first group included good-semen quality (GS; n=3), whereas, the second group included poor-semen quality (PS; n=5). The differences between GS and BS groups in all seminal biochemical attributes lacked significance.  A negative and significant (P≤0.05) correlation coefficient was noticed between cholesterol concentration and both principal and terminal sperms abnormality (r= -0.52), and total abnormalities (r= -0.53). A positive and significant (P≤0.05) correlation coefficient was shown between cholesterol concentration and normal sperm percentage (r= 0.53). Moreover, there was a negative and significant correlation (P≤0.05) between the triglyceride concentration and each of the percentages of individual sperm motility (r = -0.50) and of live sperm (r = -0.51).  It can be concluded that some biochemical characteristics of seminal plasma can be used to predict the semen quality and fertility of Iraqi buffalo bulls.
本研究旨在找出伊拉克水牛(Bubalus bubalis)精浆中的一些生化特征与精液质量之间的关系。精液用特里斯扩展剂稀释,并根据精子细胞的单个运动能力分为两组。第一组包括精子质量好(GS;n=3),第二组包括精子质量差(PS;n=5)。GS 组和 BS 组在所有精液生化属性上的差异均不显著。 胆固醇浓度与主精子和末端精子畸形率(r= -0.52)和总畸形率(r= -0.53)之间呈显著负相关(P≤0.05)。胆固醇浓度与正常精子百分比(r= 0.53)之间呈显着性正相关(P≤0.05)。此外,甘油三酯浓度与单个精子活力百分比(r=-0.50)和活精子百分比(r=-0.51)之间呈显著负相关(P≤0.05)。 由此可以得出结论,精浆的某些生化特征可用来预测伊拉克水牛公牛的精液质量和繁殖力。
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引用次数: 2
THE ROLE OF CHLOROPHYLL SPRAYING ACCORDING TO THE EVOLUTIONARY STANDARD ZADOKS IN THE GROWTH CHARACTERISTICS OF TWO CULTIVARS OF BREAD WHEAT 根据 "扎多克 "进化标准喷洒叶绿素对两种面包小麦品种生长特性的影响
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/w1877d96
M. F. H. Al-Hassan, H. Baqir, J. W. Mahmood, Assist. Prof Assist, Prof Assist, Lecturer Dept, Field Crops, Coll, Agric, Eng, Sci.
A pilot study in the field was conducted within the study station of the Field Crops Department / College of Agricultural Engineering Sciences / “University of Baghdad during winter season of 2022-2023” .In order to find out the role of chlorophyll nutrient spraying on some growth traits of two varieties of bread wheat. Using RCBD design. The results showed a significant superiority of the V1 variety in all studied traits such as (Plant height measured in centimeters, number of tillers per square meter, and  dry weight flowering100%, “the growth rate of the crop is g.m2.d-1, the hay yield in “ton .ha-1 biological yield ton. ha-1”, flag leaf area (cm2), chlorophyll concentration (SPAD), dry weight flag leaf g. ,  flag leaf' specific gravity (g.), spike length (cm) which amounted to (100.04 cm, 281.34 g .m2.d-1, 242 .06 g.m2.d-1, 5.96 ton. ha, 11.79 ton .ha , 32.68 cm, 48.73 SPAD , 0.131 g. , 0.0039 g. and 12.13 cm). respectively . comparative V2 . chlorophyll nutrient concentrations showed that the level of Ch1 (1500) mg. L-1 represented a considerable improvement in most of which comprise the following attributes “plant height cm”, “number of tillers m2”, dry weight at flowering 100% g.m2, “crop growth rate g. m2.d-1, yield of straw ton. ha., The biological yield ton. ha., flag leaf area (cm), chlorophyll concentrations (SPAD) and spike length (cm) that reached (93.15 cm, 315.00 tillers m2, 257.08 g.m2, 1.904 g.m2, 5.96 ton. ha., 12.85 ton. ha. , 29.30 cm, and 12.07 cm) respectively, compared to the concentrations of Ch2 and Ch3.
2022-2023 年冬季,在巴格达大学农业工程科学学院大田作物系研究站内进行了一项田间试验研究,目的是了解叶绿素营养喷洒对两个面包小麦品种某些生长特性的影响。采用 RCBD 设计。结果表明,V1 品种在所有研究性状上都有明显优势,如(株高以厘米为单位、每平方米分蘖数、开花干重 100%、作物生长速率 g.m2.d-1、干草产量吨.公顷-1、生物产量吨.公顷-1、旗叶面积(平方厘米)、叶绿素浓度(SPAD)、旗叶干重 g.m2.d-1、旗叶比重(g.m2.叶绿素营养浓度比较 V2 表明,Ch1(1500)mg.L-1)的水平显著提高了大部分指标,包括 "株高厘米"、"分蘖数平方米"、开花干重 100% g.m2、"作物生长速率 g.m2.d-1、秸秆产量吨/公顷"、生物产量吨/公顷、旗叶面积(厘米)、叶绿素浓度(SPAD)和穗长(厘米)分别达到 93.15 厘米、315.00 分蘖平方米、257.08 克平方米、1.904 克平方米、5.96 吨/公顷、12.85 吨/公顷、29.30 厘米和 12.85 厘米。与 Ch2 和 Ch3 的浓度相比,分别达到了 93.15 厘米、315.00 分蘖平方米、257.08 克平方米、1.904 克平方米、5.96 吨公顷、12.85 吨公顷和 29.30 厘米。
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引用次数: 0
ESTIMATING OFF –FARM LABOR SUPPLY AND ANALYSING RELATIONSHIP BETWEEN RISK AND FARM SIZE 估算农场外劳动力供应,分析风险与农场规模之间的关系
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/afwgrz97
Awf A. A E. H. Ali, A. S. S. Lecturer, Prof. Lecturer Dept. of agri. Eco. Coll, of Agri, Eng, Scie
This study was aimed to estimate  off-farm labor supply model.  Data were collected randomly from 267 wheat producers in Salah El-Din province for the year 2020, 67.4% of them are produced using pivot sprinklers for irrigation and with three tenure categories (60,80,12) dunums. Furthermore. The KS coefficient was used to analyze the producers' risk-taking behavior after estimating the production function and determining the area variable that has the most influence on the production process. If it increased by 1%, production would increase by 0.802%. The farmer's decision to adopt the technology was based on economic, social, and institutional factors. It turns out that 40% of farmers make their decision based on financing. When analyzing the decisions of farmers under risk, it was found that 35% of them make the decision in relation to the price, which is the main incentive for production. In order to understand the relationship between the risk, the return, and the size of the farm, it was found that when the area is increased, the return increases, and the revenue of a dunum at the tenure size of 10 dunams amounted to 481,600 dinars and at a higher KS level, what is known as risk-haters, the revenue was the highest possible and the risk also increased with it, and the farmers became among the large holdings they prefer. In any case, some farmers believe that when you want to get a higher return, it is important to keep in mind that there is a greater risk. Risk is affected by a number of factors, including economic, divided into price and productivity, social ones, and others related to the farmer himself in terms of efficiency, management, skill, and experience. . The research recommended a review of tenure laws and the development of risk management strategies by providing adequate funding that ensures ,Providing factories within rural areas that contribute to absorbing surplus production, creating price stability, as well as creating a labor market that reduces poverty in the rural area.
本研究旨在估算农场外劳动力供应模型。 研究随机收集了萨拉赫丁省 267 名小麦生产者 2020 年的数据,其中 67.4% 的生产者使用枢轴喷灌机进行灌溉,土地保有权分为三类(60、80、12)杜努姆。此外。在估算生产函数并确定对生产过程影响最大的面积变量后,使用 KS 系数分析生产者的风险承担行为。如果增加 1%,产量将增加 0.802%。农民决定采用该技术是基于经济、社会和制度因素。事实证明,40% 的农民是根据融资情况做出决定的。在分析农民在风险下的决策时,发现 35% 的农民是根据价格做出决策的,而价格是生产的主要激励因素。为了了解风险、收益和农场规模之间的关系,研究发现,当面积增加时,收益也随之增加,在 10 德南的保有规模下,一德南的收益达到 481 600 第纳尔,而在更高的 KS 水平(即所谓的风险厌恶者)下,收益可能是最高的,风险也随之增加,农民成为他们更喜欢的大面积保有者之一。无论如何,一些农民认为,当你想获得更高的收益时,必须牢记存在更大的风险。风险受多种因素的影响,包括经济因素(分为价格和生产率)、社会因素以及与农民本身的效率、管理、技能和经验有关的其他因素。.研究建议对土地保有权法律进行审查,并通过提供充足的资金来制定风险管理策略,以确保在农村地区建立工厂,从而吸收过剩的生产,实现价格稳定,并建立劳动力市场,减少农村地区的贫困现象。
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引用次数: 0
EFFECT OF SPRAYING BA AND ZN AT VEGETATIVE AND ROOT SYSTEM GROWTH OF PLUM SAPLINGS 喷洒钡和锌对李树苗植被和根系生长的影响
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/pc2j7e81
A. Y. Salih, A. Q. Hamdan, S. M. Tarkan
This study was aimed to investigate effects of Benzyl Adenine and Zinc sulfate on Plum trees growth, at the field of Depart. Horticultural and Landscape, College of Agricultural Engineering Sciences, University of Baghdad, during the spring season of 2021. Spraying three different concentrations (0, 50, and 100) mg.L-1 of Benzyl adenine and foliar applications of Zinc sulfate with three different concentrations (0, 25, and 50) mg.L-1 on vegetative and root system traits, as well as the chemical content of Plum seedlings (Prunus domestica L.) grafted on to Apricot rootstocks. The experiment conducted using factorial experiment with a Randomized Complete Block Design and, three replicates, Two seedlings per treatment, were used. The results revealed significant differences concerning the foliar application of Benzyl Adenine, particularly at the 100 mg/L concentration. which had a substantial impact on various vegetative traits. Additionally, it exhibited a superior effect on root characteristics.. Conversely, Zinc element demonstrated significant superiority at the concentration of 50 mg/L concerning the same vegetative and root traits,.
本研究旨在调查苄基腺嘌呤和硫酸锌对李树生长的影响。2021 年春季,巴格达大学农业工程科学学院园艺与景观系的田间,研究了苄基腺嘌呤和硫酸锌对李树生长的影响。喷洒三种不同浓度(0、50 和 100)mg.L-1 的苄基腺嘌呤和叶面喷洒三种不同浓度(0、25 和 50)mg.L-1 的硫酸锌对嫁接在杏树砧木上的李子树(Prunus domestica L.)幼苗的植株和根系性状以及化学成分的影响。实验采用随机完全区组设计的因子实验,每个处理两个幼苗,三个重复。结果表明,叶面喷施苄基腺嘌呤(尤其是浓度为 100 mg/L 的苄基腺嘌呤)对各种植株性状有重大影响。此外,苄基腺嘌呤对根系特征也有很好的影响。相反,锌元素在 50 毫克/升的浓度下,对相同的植株和根系特征表现出明显的优势。
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引用次数: 0
EFFECT OF REPLACING TREATED AND UNTREATED CORN IMPURITIES WITH UREA INSTEAD OF WHEAT BRAN ON PRODUCTIVE PERFORMANCE OF AWASSI LAMBS 用尿素代替麦麸替代处理过和未处理过的玉米杂质对阿瓦西羔羊生产性能的影响
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/zr148430
Thaer A. M. Al-Mamouri, J. A. T. Al-Ani
The study was conducted to study the effect of replacing different levels of treated and untreated corn impurities with urea instead of wheat bran on productive performance of Awassi lambs. Wheat bran were replaced by five levels of treated and untreated corn impurities as: 44:0%, 32:11%, 20:22%, 10:32% and 0:39% bran: impurities in a 2×5 factorial experiment. Concentrated fed at 3% of live body weight as dry matter basis, while alfalfa hay was offered ad libitum. Forty Awassi lambs were used, with an initial weight of 27.45 ± 2.16 kg and 4-5 months old. All lambs were fed individually feeding for 70 days, preceded by 14 days as adaptation period. Results showed a non-significant superiority in dry matter and nutrients intake by increasing corn impurities and a highly significant increased (p<0.01) in ether extract and ash intake (g/day), with superiority of digestibility (P<0.05) of organic matter% (OM) and crude protein% (CP), feed efficiency, daily gain (g/ day) and total weight gain (kg) for feeding 30% of corn impurities treated with urea (T9). In conclusion, it is possible to use corn impurities instead of wheat bran, preferably treated with urea to increase crude protein content, degraded crude fibers and improved nutritional values.
本研究旨在探讨用尿素代替小麦麸皮,取代不同含量的经处理和未经处理的玉米杂质对阿瓦西羔羊生产性能的影响。小麦麸皮被五种水平的处理过和未处理过的玉米杂质替代,分别为在 2×5 因式试验中,用 44:0%、32:11%、20:22%、10:32% 和 0:39% 的麸皮:杂质取代小麦麸皮。以活体重 3% 的干物质为基础进行精饲,同时自由采食苜蓿干草。使用了 40 只初始体重为 27.45 ± 2.16 千克、4-5 个月大的阿瓦西羔羊。所有羔羊均单独饲喂 70 天,之前 14 天为适应期。结果表明,增加玉米杂质对干物质和营养物质摄入量的影响不显著,而对醚提取物和灰分摄入量(克/天)的影响则非常显著(P<0.01),用尿素处理 30% 的玉米杂质(T9)对有机物%(OM)和粗蛋白%(CP)的消化率(P<0.05)、饲料效率、日增重(克/天)和总增重(千克)的影响也非常显著(P<0.05)。总之,可以用玉米杂质代替麦麸,最好用尿素处理,以增加粗蛋白含量、降解粗纤维和提高营养价值。
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引用次数: 0
IMPROVING NUTRITIONAL AND QUALITATIVE PROPERTIES OF WHEAT BREAD BY USING MALLOW (MALVA NEGLECTA L.) LEAVES POWDER 使用锦葵叶粉改善小麦面包的营养和质量特性
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/8p73pr77
Ezuldeen K. Hammoud, Ahmed C. Saddam, J. M. N. L. Lecturer
This study  was investigates the effect of adding different concentrations (1, 2, 3, 4, 5, 6, 7, 8) % of mallow (Malva  neglecta L.) leaves powder (MLP) to wheat bread mix on nutritional and qualitative properties of the obtained bread. Results of the chemical composition based by dry matter, moisture, ash, protein, fat, and carbohydrates for wheat flour and mallow leaves powder were 87.4, 12.6, 0.65, 10.5, 1.25, 75% and 96.2, 3.8, 7.90, 12.70, 1.60, 74.20 %, respectively. The study included nine treatments (T1, T2, T3, T4, T5, T6, T7, T8, T9 ), where T1 was the control treatment. During the fermentation period, dough  mass was  increased to its maximum value, T1 recorded the lowest height. The addition of (MLP) led to a slight increase in the specific volume of (T2, T3, T4, T5, T6), and this may be due to the increase in the proportion of polysaccharides in the mallow plant, while the specific volume began to decrease significantly in treatments (T7, T8, T9). Each of (T7, T8, and T9) showed significant differences as compared with the control treatment (T1) for the characteristics of color of crust, symmetry of form, evenness of bake, grain, color, texture of crumb,  aroma, and taste. There were no significant differences between (T2, T3, T4, T5, T6) compared with T1. The results indicate that the consumer accepted T2, T3, T4, and T5 treatments as well as T1. GC analysis showed the presence of phenolic compounds in mallow leaves powder, such as phenol, 2-chlorophenol, 2-methyl phenol, 4-methyl phenol, 2-nitrophenol, 2,4-dimethylphenol, benzoic acid, 2, 4- Dinitrophenol (0.150, 0.036, 0.03, 0.127, 1.360, 0.042, 0.002, 5.552 mg100g) respectively.
本研究调查了在小麦面包混合物中添加不同浓度(1、2、3、4、5、6、7、8)%的锦葵(Malva neglecta L.)叶粉(MLP)对所获面包的营养和质量特性的影响。小麦粉和锦葵叶粉的化学成分(干物质、水分、灰分、蛋白质、脂肪和碳水化合物)分别为 87.4%、12.6%、0.65%、10.5%、1.25%、75% 和 96.2%、3.8%、7.90%、12.70%、1.60%、74.20%。研究包括九种处理(T1、T2、T3、T4、T5、T6、T7、T8、T9),其中 T1 为对照处理。在发酵期间,面团质量增加到最大值,T1 的高度最低。添加(MLP)后,(T2、T3、T4、T5、T6)的比容略有增加,这可能是由于锦葵植物中的多糖比例增加所致,而处理(T7、T8、T9)的比容开始显著下降。与对照处理(T1)相比,处理(T7、T8 和 T9)在饼皮颜色、形状对称性、烘烤均匀度、颗粒、颜色、饼皮质地、香气和口感方面均有显著差异。T2、T3、T4、T5、T6)与 T1 相比无明显差异。结果表明,消费者对 T2、T3、T4 和 T5 处理的接受程度与 T1 相当。气相色谱分析显示锦葵叶粉中含有酚类化合物,如苯酚、2-氯苯酚、2-甲基苯酚、4-甲基苯酚、2-硝基苯酚、2,4-二甲基苯酚、苯甲酸、2,4-二硝基苯酚(分别为 0.150、0.036、0.03、0.127、1.360、0.042、0.002、5.552 mg/100g)。
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引用次数: 0
Cytotoxic Effect of Plasma Activated Medium on the Treated Breast Cancer 血浆活化介质对治疗乳腺癌的细胞毒性作用
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/bm6h2e50
Rawnaq Z. Fadhil, Mouruj A. Al, Kadhim A. Aadim Researcher Prof, BRC. Universityof Alnahrain
This study was aimed to evaluate the effect of plasma activated medium (PAM) on breast cancer cell lines (MCF7 and AMJ13) and compared it to non malignant one (ASCs). The cytotoxic effect of 10,15,20, and 25 min prepared PAM was measured and the results indicated that all cells were influenced in the exposure times (20 and 25)min, especially after 48 hours of incubation with lesser influence on normal cells. Based on the results 20 min was determined as the effective dose on 50% of cancer cells with minimal influence on normal one. Further, tumor necrosis factor-alpha (TNF-α) was assessed and the results indicated reduction in the level of this cytokine after PAM treatment, MCF7 showed significant 2reduction in both incubation intervals while AMJ13 and ASCs significantly varied only after 48hrs.Finally, caspase-9was analyzed to identify the apoptosis route and the obtained data revealed that both MCF7 and AMJ13raisedsignificantlyafter 24 and 48hrs.In contrast, ASC cells revealed non-significant changes in both intervals.
本研究旨在评估血浆活化介质(PAM)对乳腺癌细胞系(MCF7 和 AMJ13)的影响,并将其与非恶性细胞系(ASCs)进行比较。测量了 10、15、20 和 25 分钟制备的 PAM 的细胞毒性效果,结果表明,所有细胞在暴露时间(20 和 25)分钟内都受到了影响,尤其是在培养 48 小时后,对正常细胞的影响较小。根据结果,20 分钟被确定为对 50% 癌细胞有效的剂量,而对正常细胞的影响极小。此外,还对肿瘤坏死因子-α(TNF-α)进行了评估,结果表明 PAM 处理后该细胞因子水平降低,MCF7 细胞在两个培养周期内均出现显著降低,而 AMJ13 和 ASC 细胞仅在 48 小时后才出现显著变化。最后,对 Caspase-9 进行了分析,以确定细胞凋亡的途径。
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引用次数: 0
EVALUATION OF FOLIAR SPRAY WITH EXTRACT OF MARINE ALGAE AND YEAST AND MOWING DATE ON GROWTH, YIELD, AND ACTIVE COMPONENTS OF WATERCRESS. 评估叶面喷洒海藻和酵母提取物以及刈割日期对水芹生长、产量和活性成分的影响。
IF 0.6 Q3 Veterinary Pub Date : 2024-02-25 DOI: 10.36103/6310fv68
Zainab J. Al-Mousawi, Y. F. Salloom, Z. M. Abdul-Qader, Lecturer Assis. Prof, Assis. Prof
The experiment was implemented in the fields of the College of Agricultural Engineering Sciences / University of Baghdad during the agricultural season 2021-2022 as a factorial experiment (2 x 3 x 3) with within Randomized Complete Block Design using 3 replcates, The spraying with marine algae extract (0, 4, and 6ml.l-1) represented the first factor symbolized by K0, K1, and K2; the second factor involved spraying with the yeast extract (0, 2, and 4 mg.l-1) referred to as Y0, Y1, and Y2; while the third factor was mowing date as tow mows were applied with 15 days interval between them. The research was aimed to examine the effect of the three mentioned factors and their interaction on the vegetative growth and leaf biochemical traits of watercress plants. The research objectives Results showed a significant superiority of the interaction treatment Y2K2 in increasing number of leaves, leaf area, fresh weight, and dry weight in the second mow (62.80 leaves.plant-1, 131.77cm.plant-1, 265.30g, and 113.5g) as well as increasing the biochemical traits in leaves including the content of carbohydrates, protein, N, P, K, Glucosinolates, total Glucosinolates, total phenolic compounds, and total flavonoids in the second mow.                   
该实验于 2021-2022 年农季在巴格达大学农业工程科学学院的田间进行,采用随机完全区组设计的阶乘实验 (2 x 3 x 3),共设 3 个重复,喷洒海藻提取物(0、4 和 6 毫升。第二个因素是喷洒酵母提取物(0、2 和 4 mg.l-1),分别称为 Y0、Y1 和 Y2;第三个因素是割草日期,两次割草间隔 15 天。研究旨在考察上述三个因子及其交互作用对水芹植株无性生长和叶片生化性状的影响。研究结果表明,交互作用处理 Y2K2 在增加第二刈割的叶片数、叶面积、鲜重和干重(62.80 片叶/株-1、131.77 厘米/株-1、265.30 克和 113.5 克)以及增加叶片的生化性状(包括碳水化合物、蛋白质、氮、磷、钾、葡萄糖苷酸盐、总葡萄糖苷酸盐、总酚类化合物和总黄酮类化合物的含量)方面具有显著优势。
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引用次数: 0
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IRAQI JOURNAL OF AGRICULTURAL SCIENCES
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