Pub Date : 2022-10-18DOI: 10.33647/2074-5982-18-3-118-121
S. Okovitiy, E. B. Shustov
Exogenous RNAs alter interspecifically the transmission of signals in organisms by regulating the expression of their genes. This process leads to phenotypic cellular changes, thus representing a possible new tactic for the treatment of various diseases. Exogenous RNAs are a promising approach to the creation of a fundamentally new class of drugs or biologically active additives (for plant exogenous RNAs) with a promising pharmacological activity and minimal side effects.
{"title":"Exogenous RNAs as Potential Pharmacological Agents","authors":"S. Okovitiy, E. B. Shustov","doi":"10.33647/2074-5982-18-3-118-121","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-118-121","url":null,"abstract":"Exogenous RNAs alter interspecifically the transmission of signals in organisms by regulating the expression of their genes. This process leads to phenotypic cellular changes, thus representing a possible new tactic for the treatment of various diseases. Exogenous RNAs are a promising approach to the creation of a fundamentally new class of drugs or biologically active additives (for plant exogenous RNAs) with a promising pharmacological activity and minimal side effects.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"83 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75012441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-18DOI: 10.33647/2074-5982-18-3-104-108
V. Bolotova, I. A. Titovich, E. B. Shustov
The effect of succinic salt of diethylethanolamine fumaric ester on the cognitive abilities of male CBA mice and outbred male rats was studied. It was found that the test substance at doses of 10 and 75 mg/kg in the “Extrapolation escape task” test has a positive effect on the short-term and long-term memory of animals, contributing to the preservation and reproduction of the information received. In the “T-maze” test, the succinic salt of diethylethanolamine fumaric ester in both doses was superior, and in the “Extrapolation escape task” test, it had a comparable effect with the reference drug piracetam (dose 900 mg/kg).
{"title":"Effect of the Succinic Salt of Diethylethanolamine Fumar Ester on Cognitive Functions of Laboratory Animals","authors":"V. Bolotova, I. A. Titovich, E. B. Shustov","doi":"10.33647/2074-5982-18-3-104-108","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-104-108","url":null,"abstract":"The effect of succinic salt of diethylethanolamine fumaric ester on the cognitive abilities of male CBA mice and outbred male rats was studied. It was found that the test substance at doses of 10 and 75 mg/kg in the “Extrapolation escape task” test has a positive effect on the short-term and long-term memory of animals, contributing to the preservation and reproduction of the information received. In the “T-maze” test, the succinic salt of diethylethanolamine fumaric ester in both doses was superior, and in the “Extrapolation escape task” test, it had a comparable effect with the reference drug piracetam (dose 900 mg/kg).","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84561105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.33647/2074-5982-18-3-95-98
A. Machulin, I. Kosarev, V. Khlebnikov, R. Vasilenko, V. A. Samoilenko, S. Pchelintsev, V. Abramov
The consortium of symbiotic strains of lactobacilli Lactobacillus plantarum MDIIE 2165 and Lactobacillus rhamnosus MDIIE 2166, deposited in the All-Russian Collection of Microorganisms (IBFM RAS), is the most suitable starter for obtaining a fermented beet juice (FBJ). These innovative probiotic lactobacilli ensure a deep processing of raw materials and allow a specialized food product with high organoleptic characteristics, biological value and adaptogenic properties to be obtained. FBJ can be used as a specialized nutrition product to improve the performance of athletes and people working and living in extreme conditions, as well as to prolong the professional longevity of the population.
{"title":"Fermented Beet Juice with a Highly Active Symbiotic Consortium of Probiotic Lactobacillus Strains as a Specialized Product for Nutrition of People Working and Living in Extreme Conditions","authors":"A. Machulin, I. Kosarev, V. Khlebnikov, R. Vasilenko, V. A. Samoilenko, S. Pchelintsev, V. Abramov","doi":"10.33647/2074-5982-18-3-95-98","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-95-98","url":null,"abstract":"The consortium of symbiotic strains of lactobacilli Lactobacillus plantarum MDIIE 2165 and Lactobacillus rhamnosus MDIIE 2166, deposited in the All-Russian Collection of Microorganisms (IBFM RAS), is the most suitable starter for obtaining a fermented beet juice (FBJ). These innovative probiotic lactobacilli ensure a deep processing of raw materials and allow a specialized food product with high organoleptic characteristics, biological value and adaptogenic properties to be obtained. FBJ can be used as a specialized nutrition product to improve the performance of athletes and people working and living in extreme conditions, as well as to prolong the professional longevity of the population.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88541313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.33647/2074-5982-18-3-84-89
O. Galzitskaya, A. Machulin, E. I. Deryusheva, A. Glyakina, S. Grishin, S. Kurpe, A. V. Panfilov, P. Domnin, S. V. Kravchenko, S. Ermolaeva
An original approach to the development of antimicrobial peptides (AMPs) with a new mechanism of action based on directed coaggregation of a peptide with a target protein is proposed. The unique multifunctional bacterial ribosomal protein S1 was chosen as the target protein. The amyloidogenic and antibacterial effects of various peptides synthesized on the basis of S1 ribosomal protein sequences were studied. The results obtained can serve as a basis for the creation of new AMPs against various strains of pathogenic organisms.
{"title":"Antimicrobial Peptides Based on Bacterial S1 Protein Sequences as a Potential Alternative to Antibiotics","authors":"O. Galzitskaya, A. Machulin, E. I. Deryusheva, A. Glyakina, S. Grishin, S. Kurpe, A. V. Panfilov, P. Domnin, S. V. Kravchenko, S. Ermolaeva","doi":"10.33647/2074-5982-18-3-84-89","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-84-89","url":null,"abstract":"An original approach to the development of antimicrobial peptides (AMPs) with a new mechanism of action based on directed coaggregation of a peptide with a target protein is proposed. The unique multifunctional bacterial ribosomal protein S1 was chosen as the target protein. The amyloidogenic and antibacterial effects of various peptides synthesized on the basis of S1 ribosomal protein sequences were studied. The results obtained can serve as a basis for the creation of new AMPs against various strains of pathogenic organisms.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87204692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.33647/2074-5982-18-3-37-44
O. Stepanova, R. A. Klesov, K. K. Semenov, I. Pomytkin, V. Karkischenko
For the first time, LASMA ST, a device for laser diagnostic, was adapted for preclinical studies on laboratory db/db mouse genetic models of type 2 diabetes. The proposed method for studying of tissue changes during diabetes mellitus consists in a simultaneous control of microcirculation compartments: blood and lymph flow and oxidative coenzymes. The presented approach is characterized by a high informational value, safety and objectivity, as well as by the possibility of dynamic monitoring and obtaining online data on tissue metabolism (reduced nicotinamide adenine dinucleotide - NADH and oxidized flavin adenine dinucleotide - FAD).
{"title":"A New Diagnostic Approach to Assessing Tissue Changes in Type 2 Diabetes Mellitus in Mice Using “LASMA ST” Device","authors":"O. Stepanova, R. A. Klesov, K. K. Semenov, I. Pomytkin, V. Karkischenko","doi":"10.33647/2074-5982-18-3-37-44","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-37-44","url":null,"abstract":"For the first time, LASMA ST, a device for laser diagnostic, was adapted for preclinical studies on laboratory db/db mouse genetic models of type 2 diabetes. The proposed method for studying of tissue changes during diabetes mellitus consists in a simultaneous control of microcirculation compartments: blood and lymph flow and oxidative coenzymes. The presented approach is characterized by a high informational value, safety and objectivity, as well as by the possibility of dynamic monitoring and obtaining online data on tissue metabolism (reduced nicotinamide adenine dinucleotide - NADH and oxidized flavin adenine dinucleotide - FAD).","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89276708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.33647/2074-5982-18-3-58-61
I. G. Vilkova, N. S. Tropskaya, T. V. Chernen'kaya, T. S. Popova
Variations in the basic parameters of the electrical activity of the small intestine in rats were studied using an experimental model of hypochlorhydria caused by an intragastric administration of rabeprazole for 16 days. Hypochlorhydria was found to lead to an increased duration of irregular electric activity and a reduction in the period of quiescence, which indicates an increase in uncoordinated contractions of the small intestine. A decrease in the number of migrating myoelectric complexes and, consequently, a partial suppression of propulsive peristalsis contributes to an excessive bacterial overgrowth in the proximal small intestine.
{"title":"Small Bowel Electrical Activity in an Experimental Model of Hypochlorhydria","authors":"I. G. Vilkova, N. S. Tropskaya, T. V. Chernen'kaya, T. S. Popova","doi":"10.33647/2074-5982-18-3-58-61","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-58-61","url":null,"abstract":"Variations in the basic parameters of the electrical activity of the small intestine in rats were studied using an experimental model of hypochlorhydria caused by an intragastric administration of rabeprazole for 16 days. Hypochlorhydria was found to lead to an increased duration of irregular electric activity and a reduction in the period of quiescence, which indicates an increase in uncoordinated contractions of the small intestine. A decrease in the number of migrating myoelectric complexes and, consequently, a partial suppression of propulsive peristalsis contributes to an excessive bacterial overgrowth in the proximal small intestine.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90551004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.33647/2074-5982-18-3-79-83
N. S. Benderskiy, A. Safronenko, O. M. Kudelina, E. Gantsgorn, A. V. Krishtopa, A. O. Golubeva, S. Babyuk
The group of natural organic compounds referred to as humic substances are increasingly attracting attention both in Russia and globally. These compounds exhibit a fairly extensive range of biological properties, thus finding successful application in animal husbandry, agriculture and veterinary medicine. Fulvic acid represents one of the most prominent representatives of this group of substances. The chemical and biological properties of fulvic acid make it a promising candidate for application in traditional medicine and as a basis for the development of modern pharmacological preparations.
{"title":"Biological activity of fulvic acid: prospects of application in medicine","authors":"N. S. Benderskiy, A. Safronenko, O. M. Kudelina, E. Gantsgorn, A. V. Krishtopa, A. O. Golubeva, S. Babyuk","doi":"10.33647/2074-5982-18-3-79-83","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-79-83","url":null,"abstract":"The group of natural organic compounds referred to as humic substances are increasingly attracting attention both in Russia and globally. These compounds exhibit a fairly extensive range of biological properties, thus finding successful application in animal husbandry, agriculture and veterinary medicine. Fulvic acid represents one of the most prominent representatives of this group of substances. The chemical and biological properties of fulvic acid make it a promising candidate for application in traditional medicine and as a basis for the development of modern pharmacological preparations.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91280120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.33647/2074-5982-18-3-32-36
E. Orlova, I. Kondratov, O. Ogarkov
In this work, we obtain a producer of the recombinant SARS-CoV-2 E protein and develop a system for its expression and purification in a soluble form. ELISA confirmed its antigenic properties. In COVID-19 convalescents, the level of IgG against the E protein was significantly increased in comparison with the control group of patients.
{"title":"COVID-19 Convalescents Exhibit Humoral Immunity to SARS-COV-2 Envelope (E) Protein","authors":"E. Orlova, I. Kondratov, O. Ogarkov","doi":"10.33647/2074-5982-18-3-32-36","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-32-36","url":null,"abstract":"In this work, we obtain a producer of the recombinant SARS-CoV-2 E protein and develop a system for its expression and purification in a soluble form. ELISA confirmed its antigenic properties. In COVID-19 convalescents, the level of IgG against the E protein was significantly increased in comparison with the control group of patients.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76299900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.33647/2074-5982-18-3-128-132
V. Prikhodko
In this study, L-ornithine L-aspartate and empagliflozin were evaluated in terms of their potential effects on the hippocampus-dependent memory status in non-alcoholic steatohepatitis (NASH) in C57Bl/6 mice. NASH was found to be associated with an early transient decrease in the short-term recognition memory as well as a progressive reduction in the short-term spatial memory. L-ornithine L aspartate effectively prevented the impairment of recognition, rather than spatial, memory. At the same time, empagliflozin failed to improve the memory status of the animals.
{"title":"Effects of Ornithine Aspartate and Empagliflozin on Memory Deficit Symptoms in Experimental Steatohepatitis","authors":"V. Prikhodko","doi":"10.33647/2074-5982-18-3-128-132","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-128-132","url":null,"abstract":"In this study, L-ornithine L-aspartate and empagliflozin were evaluated in terms of their potential effects on the hippocampus-dependent memory status in non-alcoholic steatohepatitis (NASH) in C57Bl/6 mice. NASH was found to be associated with an early transient decrease in the short-term recognition memory as well as a progressive reduction in the short-term spatial memory. L-ornithine L aspartate effectively prevented the impairment of recognition, rather than spatial, memory. At the same time, empagliflozin failed to improve the memory status of the animals.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89930299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-10-17DOI: 10.33647/2074-5982-18-3-62-66
D. Petrov, A. A. Ivanov, E. V. Panina, N. V. Petrova, N. A. Laryushina
The article presents a brief overview of the prospects for using the long-tailed chinchilla as a model in biomedical research.
本文对长尾栗鼠在生物医学研究中的应用前景作了简要综述。
{"title":"Chinchilla lanigera — a promising model in biomedicine","authors":"D. Petrov, A. A. Ivanov, E. V. Panina, N. V. Petrova, N. A. Laryushina","doi":"10.33647/2074-5982-18-3-62-66","DOIUrl":"https://doi.org/10.33647/2074-5982-18-3-62-66","url":null,"abstract":"The article presents a brief overview of the prospects for using the long-tailed chinchilla as a model in biomedical research.","PeriodicalId":14837,"journal":{"name":"Journal Biomed","volume":"129 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80463855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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