Pub Date : 2017-10-10DOI: 10.4172/2155-9872.1000347
Z. Li, Kenta Nagahori, S. Kawata, Yuki Ogawa, H. Miyaso, N. Qu, S. Hayashi, M. Itoh, Y. Otsuki
Biacore is a system that is extensively used to characterize the interactions between molecules in terms of their binding specificity, affinity, and kinetics. The practical procedures, however, for measurement of protein-DNA association in crude nuclear extract are yet to be defined. In the present study, DNA fragments with the least protein binding activity were identified in database for transcription factors and included in Biacore assay as control, so that the signals from non-specific binding were markedly suppressed. It was known that when analytes were purified transcription factors, the dissociation curves in Biacore sensorgrams showed exponential tendency. Further analysis showed that the interaction between ERα complex from crude nuclear extract and DNA oligos could be fitted to mono- or bi-exponential functions. Discrimination between orders of exponential function was based on the results of several statistical analyses with an average score of more than 95%. As exponential characteristics allow extrapolation of the dissociation, theoretical amount of bound anti-ERα antibodies could thus be evaluated statistically. Our procedures made Biacore a practical technique like Supershift Assay to measure protein-DNA association in crude nuclear extract with reproducible and reliable results.
{"title":"Detection of Protein-DNA Binding in Crude Nuclear Extract Using Biacore Assay","authors":"Z. Li, Kenta Nagahori, S. Kawata, Yuki Ogawa, H. Miyaso, N. Qu, S. Hayashi, M. Itoh, Y. Otsuki","doi":"10.4172/2155-9872.1000347","DOIUrl":"https://doi.org/10.4172/2155-9872.1000347","url":null,"abstract":"Biacore is a system that is extensively used to characterize the interactions between molecules in terms of their binding specificity, affinity, and kinetics. The practical procedures, however, for measurement of protein-DNA association in crude nuclear extract are yet to be defined. In the present study, DNA fragments with the least protein binding activity were identified in database for transcription factors and included in Biacore assay as control, so that the signals from non-specific binding were markedly suppressed. It was known that when analytes were purified transcription factors, the dissociation curves in Biacore sensorgrams showed exponential tendency. Further analysis showed that the interaction between ERα complex from crude nuclear extract and DNA oligos could be fitted to mono- or bi-exponential functions. Discrimination between orders of exponential function was based on the results of several statistical analyses with an average score of more than 95%. As exponential characteristics allow extrapolation of the dissociation, theoretical amount of bound anti-ERα antibodies could thus be evaluated statistically. Our procedures made Biacore a practical technique like Supershift Assay to measure protein-DNA association in crude nuclear extract with reproducible and reliable results.","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77453366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-09-27DOI: 10.15436/2476-1869.17.1555
L. Vandanjon, Deniel Maureen, P. Maya, Douzenel Philippe, S. Valérie, Bedoux Gilles, Bourgougnon Nathalie, Vandanjon Laurent, Ommega Internationals
Proliferation of the introduced brown macro alga Sargassum muticum is known as a natural and hard to control phenomenon occurring along the Atlantic coasts. The phenomenon causes serious troubles for local ecosystems including the alteration of ecosystem structure, the reduction in indigenous biodiversity and economic losses (tourism, aquaculture). However, despite the serious troubles caused by S. muticum, this species contains highly remarkable bioactive metabolites. This macro alga is at present under-exploited and the valorization of its metabolites to give a positive value to this seaweed could be a solution of ecosystemic service. Biorefinery process could be one solution to valorize S. muticum. Comprehensive knowledge concerning the biochemical composition of S. muticum and the impact of environmental factors, particularly seasons, on its composition is a prerequisite before its valorization. In this study, the biochemical composition of S. muticum was evaluated by using classic colorimetric methods based on chemical analysis and Fourier Transform Infra-Red spectrometry (FTIR), and was used as a rapid and safe method that could bring advantages in screening studies and a more comprehensive management and use of seaweed products. Our results are globally in accordance, notably for phenolic compounds, showing the relevance of the use of infrared spectrometry. Moreover, based on the absorption bands of some specific and valuable compounds shown by FTIR, there was a seasonal variation in the polysaccharides, i.e., uronic acids and sulphated compounds, together with phenolic contents of S. muticum.
{"title":"Seasonal Variation of Sargassum Muticum Biochemical Composition Determined by Fourier Transform Infra-Red Spectroscopy","authors":"L. Vandanjon, Deniel Maureen, P. Maya, Douzenel Philippe, S. Valérie, Bedoux Gilles, Bourgougnon Nathalie, Vandanjon Laurent, Ommega Internationals","doi":"10.15436/2476-1869.17.1555","DOIUrl":"https://doi.org/10.15436/2476-1869.17.1555","url":null,"abstract":"Proliferation of the introduced brown macro alga Sargassum muticum is known as a natural and hard to control phenomenon occurring along the Atlantic coasts. The phenomenon causes serious troubles for local ecosystems including the alteration of ecosystem structure, the reduction in indigenous biodiversity and economic losses (tourism, aquaculture). However, despite the serious troubles caused by S. muticum, this species contains highly remarkable bioactive metabolites. This macro alga is at present under-exploited and the valorization of its metabolites to give a positive value to this seaweed could be a solution of ecosystemic service. Biorefinery process could be one solution to valorize S. muticum. Comprehensive knowledge concerning the biochemical composition of S. muticum and the impact of environmental factors, particularly seasons, on its composition is a prerequisite before its valorization. In this study, the biochemical composition of S. muticum was evaluated by using classic colorimetric methods based on chemical analysis and Fourier Transform Infra-Red spectrometry (FTIR), and was used as a rapid and safe method that could bring advantages in screening studies and a more comprehensive management and use of seaweed products. Our results are globally in accordance, notably for phenolic compounds, showing the relevance of the use of infrared spectrometry. Moreover, based on the absorption bands of some specific and valuable compounds shown by FTIR, there was a seasonal variation in the polysaccharides, i.e., uronic acids and sulphated compounds, together with phenolic contents of S. muticum.","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"42 1","pages":"75-84"},"PeriodicalIF":0.0,"publicationDate":"2017-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88036604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-31DOI: 10.4172/2155-9872.1000377
E. Taylan, G. SemraTuncel
Seasonal and spatial variation and sources of phase-distributed polycyclic aromatic hydrocarbons (PAHs) in Northwestern part of Turkey was studied for pollution level and sources appointment. Daily sampling of air was done for one year in two sites; BalA±kesir city and the dam lake. Samples were analyzed by using GC-MS. The sum of the total concentration of PAHs was 517 ± 425 ng/ml for city and 153 ± 108 ng/ml dam lake respectively. Seasonal variations were observed sharply, for the city winter highest of 700 ng/ml. Highest observed concentration for Dam Lake was 160 ng/ml in summer season. Gas to particle phase partitioning was calculated and dam lake data was more favoring for the study. In summer gas/particle ratio was 38. We have observed in city low molecular weight PAHs were in the gas phase. In winter Gas/particle ratio was in between 2-5. Diagnostic ratio analysis and factor analysis indicated combustion and vehicular emission as the major sources for PAHs pollution.
{"title":"Seasonal and Spatial Patterns of Phase-Segregated Atmospheric PolycyclicAromatic Hydrocarbons (PAHs) in North Western Part of Turkey Analysedby GC-MS","authors":"E. Taylan, G. SemraTuncel","doi":"10.4172/2155-9872.1000377","DOIUrl":"https://doi.org/10.4172/2155-9872.1000377","url":null,"abstract":"Seasonal and spatial variation and sources of phase-distributed polycyclic aromatic hydrocarbons (PAHs) in Northwestern part of Turkey was studied for pollution level and sources appointment. Daily sampling of air was done for one year in two sites; BalA±kesir city and the dam lake. Samples were analyzed by using GC-MS. The sum of the total concentration of PAHs was 517 ± 425 ng/ml for city and 153 ± 108 ng/ml dam lake respectively. Seasonal variations were observed sharply, for the city winter highest of 700 ng/ml. Highest observed concentration for Dam Lake was 160 ng/ml in summer season. Gas to particle phase partitioning was calculated and dam lake data was more favoring for the study. In summer gas/particle ratio was 38. We have observed in city low molecular weight PAHs were in the gas phase. In winter Gas/particle ratio was in between 2-5. Diagnostic ratio analysis and factor analysis indicated combustion and vehicular emission as the major sources for PAHs pollution.","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74300402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-08-29DOI: 10.4172/2155-9872.1000375
Lian-lin Su, Lijun Wang, Ping Li, Chunqin Mao, Min Hao, T. Lu
Schisandra chinensis (SC) is a well-known traditional Chinese herbal medicine that has been used in clinical practices for thousands of years. However, the differences between the effects of unprocessed and vinegarprocessed Schisandra chinensis (VSC) on cytochrome P450 (CYP450) activities are poorly understood. To evaluate the differences between processed and unprocessed SC on the metabolism of CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19 and CYP2D6 substrates in rats using a cocktail method based on a developed and validated high performance liquid chromatography-mass spectrometry (HPLC-MS) method. Six probe substrates (coumarin (CYP2A6), bupropion (CYP2B6), paclitaxel (CYP2C8), tolbutamide (CY2C9), omeprazole (CYP2C19) and metoprolol (CYP2D6)) were delivered simultaneously into rats treated with single or multiple doses of processed or crude SC extract. The plasma concentrations of the six probes were profiled by HPLC-MS, and their corresponding pharmacokinetic parameters were calculated. Treatment with single or multiple doses of either extract of SC or VSC induced CYP2A6, CYP2B6 and CYP2C9 enzyme activity and inhibited CYP2D6, CYP2C19 and CYP2C8 enzyme activity in rats. Furthermore, the inhibitory or induced effect of multiple doses of SC was more potent after vinegar processing than without vinegar processing. CYP2A6, 2B6 and 2C9 enzyme activity were induced significantly after treatment with multiple doses but not after a single dose. CYP2C19 enzyme activity were inhibited significantly after treatment with multiple doses but not after a single dose. These results provide useful scientific data for the safe clinical application of either extract of SC in combination with other drugs, which should lack the side effects induced by other herb-drug interactions.
{"title":"Effects of Unprocessed Versus Vinegar-Processed Schisandra chinensison the Activity of CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19 andCYP2D6 Enzymes in Rats","authors":"Lian-lin Su, Lijun Wang, Ping Li, Chunqin Mao, Min Hao, T. Lu","doi":"10.4172/2155-9872.1000375","DOIUrl":"https://doi.org/10.4172/2155-9872.1000375","url":null,"abstract":"Schisandra chinensis (SC) is a well-known traditional Chinese herbal medicine that has been used in clinical practices for thousands of years. However, the differences between the effects of unprocessed and vinegarprocessed Schisandra chinensis (VSC) on cytochrome P450 (CYP450) activities are poorly understood. To evaluate the differences between processed and unprocessed SC on the metabolism of CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19 and CYP2D6 substrates in rats using a cocktail method based on a developed and validated high performance liquid chromatography-mass spectrometry (HPLC-MS) method. Six probe substrates (coumarin (CYP2A6), bupropion (CYP2B6), paclitaxel (CYP2C8), tolbutamide (CY2C9), omeprazole (CYP2C19) and metoprolol (CYP2D6)) were delivered simultaneously into rats treated with single or multiple doses of processed or crude SC extract. The plasma concentrations of the six probes were profiled by HPLC-MS, and their corresponding pharmacokinetic parameters were calculated. Treatment with single or multiple doses of either extract of SC or VSC induced CYP2A6, CYP2B6 and CYP2C9 enzyme activity and inhibited CYP2D6, CYP2C19 and CYP2C8 enzyme activity in rats. Furthermore, the inhibitory or induced effect of multiple doses of SC was more potent after vinegar processing than without vinegar processing. CYP2A6, 2B6 and 2C9 enzyme activity were induced significantly after treatment with multiple doses but not after a single dose. CYP2C19 enzyme activity were inhibited significantly after treatment with multiple doses but not after a single dose. These results provide useful scientific data for the safe clinical application of either extract of SC in combination with other drugs, which should lack the side effects induced by other herb-drug interactions.","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"175 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76043664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-25DOI: 10.4172/2155-9872.1000373
W. AbdulkaderRasheed, T. FirasMaher, H. AkeelAlAisse
This study was conducted to purification G6PD enzyme from diabetic patients by using simple and cheap method the technique gel filtration on Sephadex G100 and determine molecular weight of enzyme and compare it with true molecular weight of enzyme and determine kinetic constant (Km, Vmax) and study the effect of temperature and substrate and pH and known the best condition to give optimum work of enzyme. study contain (60) patients with diabetes and (60) control Glucose and activity of G6PD were measured and the enzyme precipitated by Ammonium Sulfate with concentration (75%) and purification enzyme gel filtration on Sephadex G-100 with dimensions (1.5 × 30) cm and using the buffer solution from (Tris-HCl) at pH 8.2 to isolate the enzyme and determine molecular weight with same method. Specific activity was calculated (21.5 UI/mg), total activity (706.8 UI), number of purification (3.45) enzyme yield (23.188%) and enzyme activity (17.67 UI/ml). and the molecular weight was calculated with using same technique (57.82) kD. Effect of increased concentration of substrate on enzyme activity and found the activity increase with increase substrate and amount constant level not change however increase of concentration of substrate when drawing relation between activity and concentration of substrate format appear exchange excess and after study effect of pH found the optimum value (8.4) and study effect of temperature on activity found (38 C) the optimum temperature. Study of kinetic constant was done and the and the Michaelis-Menten (Km) value was (3.8 mM) and Vmax value (8 IU/ml).
{"title":"Purification, Determination Molecular Weight and Study KineticProperties of G6PD from Diabetes Patient","authors":"W. AbdulkaderRasheed, T. FirasMaher, H. AkeelAlAisse","doi":"10.4172/2155-9872.1000373","DOIUrl":"https://doi.org/10.4172/2155-9872.1000373","url":null,"abstract":"This study was conducted to purification G6PD enzyme from diabetic patients by using simple and cheap method the technique gel filtration on Sephadex G100 and determine molecular weight of enzyme and compare it with true molecular weight of enzyme and determine kinetic constant (Km, Vmax) and study the effect of temperature and substrate and pH and known the best condition to give optimum work of enzyme. study contain (60) patients with diabetes and (60) control Glucose and activity of G6PD were measured and the enzyme precipitated by Ammonium Sulfate with concentration (75%) and purification enzyme gel filtration on Sephadex G-100 with dimensions (1.5 × 30) cm and using the buffer solution from (Tris-HCl) at pH 8.2 to isolate the enzyme and determine molecular weight with same method. Specific activity was calculated (21.5 UI/mg), total activity (706.8 UI), number of purification (3.45) enzyme yield (23.188%) and enzyme activity (17.67 UI/ml). and the molecular weight was calculated with using same technique (57.82) kD. Effect of increased concentration of substrate on enzyme activity and found the activity increase with increase substrate and amount constant level not change however increase of concentration of substrate when drawing relation between activity and concentration of substrate format appear exchange excess and after study effect of pH found the optimum value (8.4) and study effect of temperature on activity found (38 C) the optimum temperature. Study of kinetic constant was done and the and the Michaelis-Menten (Km) value was (3.8 mM) and Vmax value (8 IU/ml).","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85257476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-25DOI: 10.4172/2155-9872.1000374
Emran Eisa Saleh, H. I. Kassas, S. E. Fiki, H. Diab, Ahmed Ali Saeed Al Nagashee
The knowledge of environmental hazards resulted from natural radioactivity and human activities is very important for monitoring of environmental contamination. In this study, the environmental hazards due to the natural radioactivity and human activities in Tuban delta in Yemen were discussed and evaluated using gamma ray spectroscopy. Thirty soil samples were collected from the study area. The results showed that the mean activity concentrations of 238U series (226Ra), 232Th, and 40K were 16.83 ± 2.3, 24.76 ± 2.3, 646.48 ± 13 Bq kg-1 and 15.22 ± 1.5, 21.99 ± 2.1, 472.58 ± 10 Bq kg-1 for farm soil samples treated with organic and urea fertilizer and farm soil samples treated with urea fertilizer, respectively. The corresponding values were 19.53 ± 1.5, 24.46 ± 3, and 500.76 ± 17 Bq kg-1 for uncultivated soil samples. The activity concentrations of 137Cs showed a significant value in some uncultivated soil samples. Also, physical and chemical properties of some soil samples were discussed and evaluated. A Radiological parameter, an absorbed dose rate in air, and an annual effective dose were calculated and evaluated. The results were compared with those of literature.
{"title":"Evaluation of Environmental Hazards Resulted from Human Activities andNatural Radioactivity in Tuban Delta in Yemen","authors":"Emran Eisa Saleh, H. I. Kassas, S. E. Fiki, H. Diab, Ahmed Ali Saeed Al Nagashee","doi":"10.4172/2155-9872.1000374","DOIUrl":"https://doi.org/10.4172/2155-9872.1000374","url":null,"abstract":"The knowledge of environmental hazards resulted from natural radioactivity and human activities is very important for monitoring of environmental contamination. In this study, the environmental hazards due to the natural radioactivity and human activities in Tuban delta in Yemen were discussed and evaluated using gamma ray spectroscopy. Thirty soil samples were collected from the study area. The results showed that the mean activity concentrations of 238U series (226Ra), 232Th, and 40K were 16.83 ± 2.3, 24.76 ± 2.3, 646.48 ± 13 Bq kg-1 and 15.22 ± 1.5, 21.99 ± 2.1, 472.58 ± 10 Bq kg-1 for farm soil samples treated with organic and urea fertilizer and farm soil samples treated with urea fertilizer, respectively. The corresponding values were 19.53 ± 1.5, 24.46 ± 3, and 500.76 ± 17 Bq kg-1 for uncultivated soil samples. The activity concentrations of 137Cs showed a significant value in some uncultivated soil samples. Also, physical and chemical properties of some soil samples were discussed and evaluated. A Radiological parameter, an absorbed dose rate in air, and an annual effective dose were calculated and evaluated. The results were compared with those of literature.","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"6 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82049945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-21DOI: 10.4172/2155-9872.1000372
P. Bansal, G. Gaurav, N. Nidhi, S. Rani, A. Malik
A new method for quantification of twelve nitroaromatic compounds including 2,4,6-TNT, its metabolites and Tetryl with microextraction by packed sorbent (MEPS), followed by gas chromatography - mass spectrometric (GC-MS) detection in environmental and biological samples is developed. MEPS employ 4 mg of C18 silica sorbent inserted into a micro-volume syringe for sample preparation. Several parameters capable of influencing the microextraction procedure viz., number of extraction cycles, washing solvent, volume of washing solvent, elution solvent, elution solvent volume and pH of matrix etc., were optimized. Helium gas was used as mobile phase during GC operation. The developed method produced satisfactory results with excellent values of coefficient of determination (R2>0.9804) within the established calibration range. The extraction yields were satisfactory for all analytes (>89.32%) for aqueous samples and (>87.45%) for biological samples. The limits of detection values lie in the range 14 - 828 pg/mL. Due to procedural simplicity, high sensitivity and efficient resolution of all analytes, the developed method was applied successfully for quantification of nitro aromatic explosives in real aqueous and fluidic biological samples.
{"title":"Development of Microextraction by Packed Sorbent - Gas Chromatography-MassSpectrometry Method for Quantification of Nitro-Explosives in Aqueous andFluidic Biological Samples","authors":"P. Bansal, G. Gaurav, N. Nidhi, S. Rani, A. Malik","doi":"10.4172/2155-9872.1000372","DOIUrl":"https://doi.org/10.4172/2155-9872.1000372","url":null,"abstract":"A new method for quantification of twelve nitroaromatic compounds including 2,4,6-TNT, its metabolites and Tetryl with microextraction by packed sorbent (MEPS), followed by gas chromatography - mass spectrometric (GC-MS) detection in environmental and biological samples is developed. MEPS employ 4 mg of C18 silica sorbent inserted into a micro-volume syringe for sample preparation. Several parameters capable of influencing the microextraction procedure viz., number of extraction cycles, washing solvent, volume of washing solvent, elution solvent, elution solvent volume and pH of matrix etc., were optimized. Helium gas was used as mobile phase during GC operation. The developed method produced satisfactory results with excellent values of coefficient of determination (R2>0.9804) within the established calibration range. The extraction yields were satisfactory for all analytes (>89.32%) for aqueous samples and (>87.45%) for biological samples. The limits of detection values lie in the range 14 - 828 pg/mL. Due to procedural simplicity, high sensitivity and efficient resolution of all analytes, the developed method was applied successfully for quantification of nitro aromatic explosives in real aqueous and fluidic biological samples.","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79204996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-20DOI: 10.4172/2155-9872.1000371
Marion Marlinge, Donato Vairo, Viviana Marolda, L. Bruzzese, N. Adjriou, C. Guiol, Nathalie Kipson, Anna Bonnardel, M. Gastaldi, F. Kerbaul, P. Michelet, J. Deharo, G. Mottola, P. Mace, M. Chefrour, R. Guieu
Background: Adenosine is a nucleoside that impacts the cardiovascular system during cardiovascular or inflammatory diseases. The rapid determination of adenosine in blood may be useful in emergency medicine especially in syncope diagnose or septic shock. We compare its measurement in blood using fixed potential amperometry (FPA), with usual methods: mass spectrometry (LC-MS/MS) or high performance liquid chromatography (HPLC). Methods: Twenty healthy subjects (14 men and 6 women) and ten patients suffering from vasovagal syncope (VVS, 6 women and 4 men) were included. Blood samples were collected by vein puncture for plasma adenosine assay and in the same time using finger puncture for direct FAP measurement and on blotting paper for LC-MS/MS. Results: Mean plasma adenosine concentration was 26% higher using HPLC compared with LC-MSMS; p<0.01. In whole blood, adenosine concentration was 35% higher using FPA compared with LC-MS/MS. We found a good correlation between adenosine values measured by FAP and LC-MS/MS in whole blood and between LCMS/ MS and HPLC in plasma. Mean adenosine concentration was higher in patients whatever the method used. Conclusion: Adenosine measurement to the patient’s bed, using FPA may be useful in some cases where high adenosine is associated with pejorative outcome.
{"title":"Rapid Measurement of Adenosine Concentration in Human Blood Using FixedPotential Amperometry: Comparison with Mass Spectrometry and High-Performance Liquid Chromatography","authors":"Marion Marlinge, Donato Vairo, Viviana Marolda, L. Bruzzese, N. Adjriou, C. Guiol, Nathalie Kipson, Anna Bonnardel, M. Gastaldi, F. Kerbaul, P. Michelet, J. Deharo, G. Mottola, P. Mace, M. Chefrour, R. Guieu","doi":"10.4172/2155-9872.1000371","DOIUrl":"https://doi.org/10.4172/2155-9872.1000371","url":null,"abstract":"Background: Adenosine is a nucleoside that impacts the cardiovascular system during cardiovascular or inflammatory diseases. The rapid determination of adenosine in blood may be useful in emergency medicine especially in syncope diagnose or septic shock. We compare its measurement in blood using fixed potential amperometry (FPA), with usual methods: mass spectrometry (LC-MS/MS) or high performance liquid chromatography (HPLC). Methods: Twenty healthy subjects (14 men and 6 women) and ten patients suffering from vasovagal syncope (VVS, 6 women and 4 men) were included. Blood samples were collected by vein puncture for plasma adenosine assay and in the same time using finger puncture for direct FAP measurement and on blotting paper for LC-MS/MS. Results: Mean plasma adenosine concentration was 26% higher using HPLC compared with LC-MSMS; p<0.01. In whole blood, adenosine concentration was 35% higher using FPA compared with LC-MS/MS. We found a good correlation between adenosine values measured by FAP and LC-MS/MS in whole blood and between LCMS/ MS and HPLC in plasma. Mean adenosine concentration was higher in patients whatever the method used. Conclusion: Adenosine measurement to the patient’s bed, using FPA may be useful in some cases where high adenosine is associated with pejorative outcome.","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"32 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2017-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87024865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-07-15DOI: 10.4172/2155-9872.1000370
Francois Gaie Levrel, N. C. Foraison, P. Gillery, V. Delatour
Nanostructured materials and their specific physical and chemical properties have been widely used over these past decades for a large range of applications, from electronics to energy, catalysis or medicine. However, for process optimization in the context of industrial production, air quality survey, biomedical applications and almost all areas where nanoparticles are involved, thorough and accurate characterization of these materials is needed [1-3]. According to the European recommendation 2011/696/EU, a nanomaterial is “a natural, incidental or manufactured material containing particles, in an unbound state or as an aggregate or as an agglomerate, and where for 50% or more of the particles in the number size distribution, one or more external dimension is in the size range 1 nm-100 nm” [4]. As evidenced by this definition, two major parameters are to be measured in order to characterize a nanostructured material and to determine whether it is considered nano or not: the particle size and the corresponding particle number concentration. However, the classification of a material should be independent of the method(s) chosen for its characterization, which implies that methods must provide comparable results.
{"title":"Establishing SI-Traceability of Nanoparticle Enumeration Techniques: A Case Study on Electrospray Differential Mobility Analysis","authors":"Francois Gaie Levrel, N. C. Foraison, P. Gillery, V. Delatour","doi":"10.4172/2155-9872.1000370","DOIUrl":"https://doi.org/10.4172/2155-9872.1000370","url":null,"abstract":"Nanostructured materials and their specific physical and chemical properties have been widely used over these past decades for a large range of applications, from electronics to energy, catalysis or medicine. However, for process optimization in the context of industrial production, air quality survey, biomedical applications and almost all areas where nanoparticles are involved, thorough and accurate characterization of these materials is needed [1-3]. According to the European recommendation 2011/696/EU, a nanomaterial is “a natural, incidental or manufactured material containing particles, in an unbound state or as an aggregate or as an agglomerate, and where for 50% or more of the particles in the number size distribution, one or more external dimension is in the size range 1 nm-100 nm” [4]. As evidenced by this definition, two major parameters are to be measured in order to characterize a nanostructured material and to determine whether it is considered nano or not: the particle size and the corresponding particle number concentration. However, the classification of a material should be independent of the method(s) chosen for its characterization, which implies that methods must provide comparable results.","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"223 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75553890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2017-06-22DOI: 10.4172/2155-9872.1000367
I. Ahmad, A. Zaman, N. Samad, M. Ayaz, Shah Rukh, A. Akbar, N. Ullah
The determination of the seven elements was performed by Perkin Elmer Atomic Absorption (AA) spectrophotometer. The present study highlights the importance of seven heavy metals residual concentration including Cd, Cr, Cu, Fe, Mn, Ni and Zn in milk of Camel, Cattle, Buffalo, Sheep and Goat from various areas of Khyber Pakhtunkhwa (KPK), Pakistan. It revealed that milk of camel comprising of high levels of Zn (5.150 ± 0.021 mg/kg), Mn (0.094 ± 0.003 mg/kg) and Fe (1.580 ± 0.530 mg/kg) with a definite correlation. In the milk of buffalo, high concentration of noxious heavy metals including Cu (0.223 ± 0.010 mg/kg) and Cd (0.117 ± 0.086 mg/kg) were found whereas in goat milk, high Ni (1.152 ± 0.045 mg/kg) and Cr (1.152 ± 0.045 mg/kg) was observed and detected. The analysis showed that camel and buffalo have similar high concentration of heavy metals. Overall results showed that milk of cattle shows higher concentration of Zn, Mn and Fe along with Buffalo.
{"title":"Atomic Absorption Spectrophotometery Detection of Heavy Metals in Milk ofCamel, Cattle, Buffalo and Goat from Various Areas of Khyber- Pakhtunkhwa(KPK), Pakistan","authors":"I. Ahmad, A. Zaman, N. Samad, M. Ayaz, Shah Rukh, A. Akbar, N. Ullah","doi":"10.4172/2155-9872.1000367","DOIUrl":"https://doi.org/10.4172/2155-9872.1000367","url":null,"abstract":"The determination of the seven elements was performed by Perkin Elmer Atomic Absorption (AA) spectrophotometer. The present study highlights the importance of seven heavy metals residual concentration including Cd, Cr, Cu, Fe, Mn, Ni and Zn in milk of Camel, Cattle, Buffalo, Sheep and Goat from various areas of Khyber Pakhtunkhwa (KPK), Pakistan. It revealed that milk of camel comprising of high levels of Zn (5.150 ± 0.021 mg/kg), Mn (0.094 ± 0.003 mg/kg) and Fe (1.580 ± 0.530 mg/kg) with a definite correlation. In the milk of buffalo, high concentration of noxious heavy metals including Cu (0.223 ± 0.010 mg/kg) and Cd (0.117 ± 0.086 mg/kg) were found whereas in goat milk, high Ni (1.152 ± 0.045 mg/kg) and Cr (1.152 ± 0.045 mg/kg) was observed and detected. The analysis showed that camel and buffalo have similar high concentration of heavy metals. Overall results showed that milk of cattle shows higher concentration of Zn, Mn and Fe along with Buffalo.","PeriodicalId":14865,"journal":{"name":"Journal of analytical and bioanalytical techniques","volume":"74 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2017-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80628126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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