Journal of animal science最新文献

{"title":"The impact of exogenous vasoactive intestinal polypeptide (VIP) on inflammatory responses and mRNA expression of tight junction genes in lambs fed a high-grain diet","authors":"Golam K Mia, Emma Hawley, Mustapha Yusuf, Samat Amat, Alison K Ward, Wanda L Keller, Glenn Dorsam, Kendall C Swanson","doi":"10.1093/jas/skae309","DOIUrl":"https://doi.org/10.1093/jas/skae309","url":null,"abstract":"This study assessed the impact of administering vasoactive intestinal polypeptide (VIP) on inflammation and intestinal VIP and tight junction mRNA expression in lambs fed grain-based finishing diets. Sixteen wether lambs (69.6 ± 1.9 kg) were individually housed, adapted to a corn-based diet containing no forage, and randomly assigned to 2 treatment groups. Lambs were intraperitoneally injected every other day for 28 d with either saline (0.9% NaCl) with no VIP (n = 8; control) or saline with VIP (n = 8; 1.3 nmol/kg BW). Blood samples were collected weekly for analysis of cytokine concentrations, and on day 0 and 28 for lipopolysaccharide (LPS), and LPS binding protein (LBP) concentrations. Upon completion of the treatment period, lambs were euthanized and gastrointestinal tissues, including rumen, jejunum, cecum, and colon samples, collected for analysis of the expression of tight junction mRNA (claudin-1, claudin-4, occludin, and ZO-1), endogenous VIP, and VIP receptor (VPAC-1). No treatment effects (P ≥ 0.38) were observed for VIP and VPAC-1 mRNA expression in colon. Supplementation with VIP did not influence (P ≥ 0.28) the expression of claudin-1, claudin-4, occludin, and ZO-1 tight junction mRNA in the rumen, jejunum, cecum, and colon. Lambs treated with VIP had greater (P ≤ 0.01) plasma concentrations of the anti-inflammatory cytokines, IL-10 and IL-36RA. There were treatment by day interactions observed (P ≤ 0.02) for concentrations of the proinflammatory cytokines, MIP-1α and MIP-1β. Lambs that did not receive VIP had greater serum concentrations of LPS (P = 0.05) than the lambs receiving VIP. These data suggest that VIP administration may not influence tight junction mRNA expression but may decrease LPS concentrations and thus inflammation in lambs fed a grain-based diet.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"9 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142430491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary fibre and weaning age affect stress and immune markers in saliva of sows and their offspring","authors":"Łukasz Grześkowiak, José Joaquín Cerón, Marina Lopez-Arjona, Beatriz Martínez-Vallespín, Johannes Schulze Holthausen, Philip Krüsselmann, Cornelia C Metges, Björn Kuhla, Wilfried Vahjen, Jürgen Zentek, Eva-Maria Saliu","doi":"10.1093/jas/skae312","DOIUrl":"https://doi.org/10.1093/jas/skae312","url":null,"abstract":"Diet, especially the intake of dietary fibre, and weaning practices may influence pig wellbeing. This study assessed changes in salivary stress and immune markers in sows and their offspring fed either hay (HAY) or sugar beet pulp (SBP), either fine (F) or coarse (C), during gestation and lactation. The effect of weaning age (conventional-CW, late-LW) on these markers was also evaluated. Saliva was analysed for chromogranin A (CgA), cortisol, alpha-amylase, oxytocin, immunoglobulin A (IgA) and adenosine deaminase (ADA). CgA was higher at CW vs. LW in sows fed SBP-F (P=0.038). Alpha-amylase was higher in sows fed HAY-C at CW vs. LW (P=0.005) and in sows fed SBP-C at LW vs. CW (P=0.096). CgA and oxytocin were higher in sows fed SBP-F at CW vs. LW (P =0.038 and P =0.017, respectively). ADA was higher in sows fed SBP-C vs. HAY-C (P=0.035) at LW and at LW vs. CW in sows fed SBP-C (P=0.002). Piglet salivary CgA was higher at CW vs. LW in HAY-F (P=0.002) and SBP-F (P=0.031). Oxytocin was higher at CW vs. LW in piglets fed HAY-F (P=0.006). Piglet salivary IgA was higher in HAY-C vs. HAY-F at CW (P=0.010) and at LW vs. CW in piglets fed HAY-F (P=0.021). ADA was higher in piglets fed SBP-F vs. HAY-F (P=0.053) at CW and SBP-F vs. SBP-C (P=0.042) at LW. Dietary fibre type, weaning age and to a lesser extent grinding degree affect stress and immune markers in pigs. These findings highlight the impact of diet and weaning practice on pig welfare.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"5 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142430478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chicken slurry inclusion on apparent total tract macronutrient digestibility, palatability, and fecal characteristics, microbiota, and metabolites of healthy adult dogs","authors":"Elizabeth L Geary, Christina L Vogel, Patrícia M Oba, Julio C Mioto, Maria R C de Godoy, Kelly S Swanson","doi":"10.1093/jas/skae313","DOIUrl":"https://doi.org/10.1093/jas/skae313","url":null,"abstract":"“Premium” pet foods are often formulated with meat slurries. Meat slurries are believed to be of higher quality than rendered meals, but inadequate research has been performed to test how their inclusion affects palatability, digestibility, or indicators of gastrointestinal health. Therefore, the objectives of this study were to determine how chicken slurry inclusion affected the palatability and apparent total tract macronutrient digestibility (ATTD) of dog foods and to assess their effects on the fecal characteristics, metabolites, and microbiota of dogs. A replicated 3x3 Latin square design digestibility study was conducted using 9 healthy adult dogs (age = 5.44 ± 0.53 yr) to test diets containing 0% (control; CON), 8% (low inclusion; LOW), and 16% (high inclusion; HIGH) chicken slurry. The experiment comprised three 21-day experimental periods (14 days of adaptation, 5 days of total fecal collection (used for ATTD calculations), and 2 days of blood collection). On the first day of fecal collections, one fresh sample was collected for measurement of pH, dry matter (DM) content, fermentative metabolite concentrations, and microbiota populations. A 2-day palatability study (n=20 dogs) was also conducted to compare CON vs. HIGH. Data were analyzed statistically by Mixed Models using SAS 9.4, with P<0.05 being significant. In the palatability study, dogs were shown to prefer (P<0.05) the HIGH diet by a ratio of 2:1. In the digestibility study, fecal output, scores, pH, and DM percentage were not different among diets. The ATTD of protein was higher (P<0.05) for the HIGH diet (84.6%) than for the LOW (82.7%) or CON (82.6%) diets. The ATTD of other nutrients and energy were not different among diets (all over 80%). Fecal propionate, butyrate, and total short-chain fatty acid concentrations were higher (P<0.05) in dogs fed the LOW diet (122.0, 67.4, and 408.2 βmol/g, respectively) than those fed the HIGH diet (89.0, 46.9, and 338.2 βmol/g, respectively). The other fecal metabolites (acetate, branched-chain fatty acids, ammonia, phenol, and indole) were not different among treatments. Few changes to the fecal microbiota were noted. However, the relative abundance of fecal Fusobacterium was higher (P<0.05) in dogs fed the CON diet than those fed the HIGH diet (25.% vs. 20.0% relative abundance). In summary, chicken slurry inclusion improved palatability but had minimal effects on nutrient digestibility and fecal characteristics, metabolites, and microbiota.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"60 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142415651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taurine enhances growth performance by improving intestinal integrity and antioxidant capacity of weaned piglets","authors":"Jianjun Chen, Xiaofeng Zhang, Yuhui Zhang, Shouchuan Jiang, Yu Han, Lei Zhang, Yuanyuan Zhang, Huahua Du","doi":"10.1093/jas/skae311","DOIUrl":"https://doi.org/10.1093/jas/skae311","url":null,"abstract":"Taurine is an amino acid that has been considered by animal husbandry as a feed additive due to its abundant biological functions. However, the effective dose of taurine added to feed is unknown. The aim of current study was to determine the optimal taurine supplementation level by investigating its effects on growth performance, diarrhea index, intestinal health and antioxidant capacity of weaned piglets. A total of 160 crossbred piglets (Landrace × Yorkshire, initially 8.39 ±0.11 kg) were assigned to 4 groups (10 pigs/pen and 4 replicates/group). Basal diets containing 0 (control, CON), 0.1%, 0.3%, and 0.5% taurine were respectively provided to the piglets for a duration of 28 days. Six piglets from each group were selected for euthanasia and subsequent sample collection on day 29. The results showed that dietary 0.3% or 0.5% taurine supplementation increased average daily gain (P < 0.05), feed to gain ratio (P < 0.01), and serum albumin (P < 0.05), and decreased diarrhea index (P < 0.01) and diamine oxidase (DAO) level in the serum (P < 0.05). The greater expression of tight junction-related genes, including ZO-1 (P < 0.05) and Claudin-1 (P < 0.01), were observed in the duodenum after supplementation with 0.5% taurine. The supplementation of 0.3% or 0.5% taurine resulted in a significant reduction of crypt depth (P < 0.01) and an increase of villus height-to-crypt depth ratio (P < 0.01) in the duodenum. A greater abundance of goblet cells was detected in the duodenum and jejunum of piglets fed 0.5% taurine (P < 0.05). In addition, serum superoxide dismutase (SOD) level, liver catalase (CAT) level and liver total antioxidant capacity level were all significantly (P < 0.05) increased with 0.1%, 0.3% or 0.5% dietary taurine supplementation. On the whole, dietary supplementation with 0.3% or 0.5% taurine has the potential to significantly enhance the growth performance of piglets by improving the integrity of the intestinal barrier and boosting their antioxidant capacity.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"229 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142430477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium deficiency modulates necroptosis-mediated intestinal inflammation in broiler through the lncRNAWSF27/miRNA1696/GPX3 axis.","authors":"Yiming Zhang,Chunyu Wei,Jiayi Ding,Jiahong Chu,Bo Huang,Guangliang Shi,Shu Li","doi":"10.1093/jas/skae288","DOIUrl":"https://doi.org/10.1093/jas/skae288","url":null,"abstract":"Deficiency of selenium (Se), an important trace element, causes diarrhea and even death in broilers, thereby affecting the economic development of poultry production. Adding Se is one way to relieve this situation; however, it has not fundamentally resolved intestinal inflammation. Therefore, we sought a new strategy to alleviate intestinal inflammation by studying the specific mechanisms of Se deficiency. By replicating the Se-deficient broiler model and establishing a chicken small intestinal epithelial cell (CSIEC) model, we determined that Se deficiency caused intestinal oxidative stress and necroptotic intestinal inflammation in broilers by decreasing glutathione peroxidase (GPX) 3 expression. Simultaneously, the expression of long non-coding RNA (lncRNA)WSF27 decreased and that of miR-1696 increased in Se-deficient intestines. Recently discovered competing endogenous RNAs (ceRNAs) form novel regulatory networks, which were found that selenoproteins involved in ceRNA regulation. However, the mechanism of action of the non-coding RNA/GPX3 axis in Se-deficient broiler intestinal inflammation remains unclear. This study aimed to explore the mechanism through which Se deficiency regulates intestinal inflammation in broilers through the lncRNAWSF27/miR-1696/GPX3 axis. Our previous studies showed that lncRNAWSF27, miR-1696, and GPX3 have ceRNA-regulatory relationships. To further determine the role of the lncRNAWSF27/miR-1696/GPX3 axis in Se-deficient broiler intestinal inflammation, CSIEC models with GPX3 knockdown/overexpression, lncRNAWSF27 knockdown, or miR-1696 knockdown/overexpression were established to simulate intestinal injury. GPX3 knockdown, as well as lncRNAWSF27 and miR-1696 overexpression, aggravated cell damage. On the contrary, it can alleviate this situation. Our results reveal that mechanism of lncRNAWSF27/miR-1696/GPX3 regulated Se-deficient broiler intestinal inflammation. This conclusion enriches our understanding of the mechanism of intestinal injury caused by Se deficiency, and contributes to the diagnosis of Se-deficient intestinal inflammation and relevant drug development.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"24 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rate of body weight gain during early gestation in F0 beef heifers has effects that extend multigenerationally to the F2 fetuses.","authors":"Friederike Baumgaertner,Germán Darío Ramírez-Zamudio,Ana Clara B Menezes,Isabella M Jurgens,Mara R Hirchert,Jennifer L Hurlbert,Kerri A Bochantin,Wellison J S Diniz,Lawrence P Reynolds,Alison K Ward,Pawel P Borowicz,S R Underdahl,James D Kirsch,Sheri T Dorsam,Kevin K Sedivec,Kendall C Swanson,Joel S Caton,Carl R Dahlen","doi":"10.1093/jas/skae295","DOIUrl":"https://doi.org/10.1093/jas/skae295","url":null,"abstract":"Our aim was to investigate the effects of maternal (F0) body weight (BW) gain during the first 84 d of gestation on body composition, ovarian reserve, and hormonal and metabolic parameters of breeding-age F1 heifers, as well as the body weight and morphometry of F2 fetuses. The study also evaluated the effect of maternal body weight gain (F0) on the mRNA relative abundance of the small intestine of both F1 heifers and F2 fetuses. Crossbred Angus heifers (F0; n = 100) were managed to gain 0.20 kg/d (low gain [LG], n = 50) or 0.75 kg/d (moderate gain [MG], n = 50) for the first 84 d of gestation. Subsequently, F0 dams were managed on a common forage-based diet for the rest of gestation until the weaning of the F1 offspring. At 15 months of age a subset of F1 heifers was randomly selected for the current experiment (n = 8 LG and n = 8 MG). Heifers were bred via artificial insemination (AI; d 0), then harvested on d 84 of gestation. On d -10, 42, and 84, BW was recorded, and blood was collected and analyzed for concentrations of glucose, non-esterified fatty acids, progesterone, insulin, and insulin-like growth factor-1. Weight of F1 carcasses, organs, gravid uteri, and F2 fetuses and organs were recorded at harvest. Visible follicles were counted on F1 ovaries at harvest, and histology was used to count microscopic follicles. Liver and jejunal samples from F1 heifers were collected to measure tissue oxygen consumption and jejunal samples from F1 heifers and F2 fetuses were collected for mRNA relative abundance analysis. Body weight of F1 heifers from MG dams tended to be 12 kg greater (P = 0.06) than for F1 heifers from LG dams. Concentrations of glucose were greater (P = 0.03) in F1 heifers from the MG group, with no differences in other blood metabolites or follicular populations (P ≥ 0.16). Interestingly, mammary glands were heavier (P = 0.05) and placentas and body depth tended to be heavier and greater, respectively (P ≤ 0.10), for F2 fetuses from F0 LG heifers. Oxygen consumption in the liver and jejunum, as well as mRNA relative abundance in the jejunum of F1 heifers, were not affected by F0 rate of gain (P ≥ 0.16). However, the NDUFC1, SDHA, UQCR1, and PPARG genes were upregulated (P ≤ 0.05) in the jejunum of F2 fetuses from the LG group. In conclusion, BW gain of F0 heifers during early gestation exerts subtle effects on pre-breeding BW and blood metabolites in F1 offspring, with impacts present in F2 placenta, mammary gland, and intestine.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"32 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142325104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary curcumin supplementation enhances growth performance and anti-inflammatory functions by modulating gut microbiota, microbiota-derived metabolites, and expression of inflammation-related genes in broilers.","authors":"Xinyu Chen,Fei Li,Xingyu Xu,Lijun Wang,Yichen Yu,Jie Yan,Xuemei Shan,Rui Zhang,Hua Xing,Tangjie Zhang,Min Du,Shifeng Pan","doi":"10.1093/jas/skae296","DOIUrl":"https://doi.org/10.1093/jas/skae296","url":null,"abstract":"Curcumin (CUR) is a natural polyphenolic substance that has been widely used since ancient times for its multiple beneficial functions. However, whether CUR affects growth performance of broilers by altering gut microbiota and metabolite and the underlying mechanism are largely unknown. This study was conducted to evaluate the effect of dietary CUR supplementation on growth performance, anti-inflammatory function, intestinal morphology and barrier, cecum microbiota and metabolite profile of broilers. Sixty 1-day-old male broilers were randomly divided into control group (CON, fed a control diet) and CUR group (fed a control diet supplemented with 200 mg/kg CUR) after 2 days of adaptation. Results showed that after feeding to 52-day-old, compared with CON broilers, the CUR broilers showed improved feed utilization efficiency and growth performance. Furthermore, the CUR broilers showed an improved intestinal morphology, which was demonstrated by a lower crypt depth in the jejunum. The 16S rRNA gene sequencing and non-targeted metabonomics (LC-MS/MS) analysis results showed that the cecum microbiota ecology and function were significantly improved, and the abundance of beneficial flora and metabolites were increased, while the harmful bacteria and metabolites were significantly decreased. In addition, RT-qPCR results showed that CUR significantly reduced inflammatory responses, promoted the formation of the mucosal barrier and enhanced digestion, absorption and transport of lipids and glucose related genes expression in the intestine. These above findings demonstrated that dietary CUR supplementation improved growth performance, intestinal morphology and anti-inflammatory functions, mainly by manipulating cecum microbiota and microbiota-derived metabolites, which provides a credible explanation for the growth-promoting effect and anti-inflammatory functions of CUR and aids our understanding of the mechanisms underlying.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"6 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142325097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of supplementing algae to breeding and early gestation first calf heifers on growth and reproduction","authors":"G T Nicholls, B J Funnell, K R Stewart, R P Lemenager, N G Briggs, J P Schoonmaker","doi":"10.1093/jas/skae282","DOIUrl":"https://doi.org/10.1093/jas/skae282","url":null,"abstract":"Fat supplementation has potential to improve reproductive performance and increase pregnancy rates in cattle by increasing the energy density of the diet. However, some of the positive effects of fat seem to be influenced by the type of fatty acid fed. Docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3) are long-chain n-3 poly-unsaturated fatty acids (PUFA) that have important biological effects on reproduction through their involvement in hormone and series 3 prostaglandin synthesis. Ruminant tissues are naturally almost devoid of n-3 long-chain PUFA, specifically EPA and DHA. Algae biomass provides a consistent source of DHA and EPA that could be fed to alter hormonal profiles and improve reproduction of beef heifers. Eighty-eight Angus × Simmental heifers (427 ± 1.8 kg BW) were blocked by BW and allotted to 2 treatments (44/treatment, 4 pens/treatment, 11 heifers/pen). Control heifers were fed a diet that contained (DM basis) 52.8% mixed grass silage, 32% corn silage, and 15.2% concentrate. DHAgold™ (49% fat; 21.8% DHA; DSM Inc.) was included in the algae diet at 1.65% of DM, replacing equal parts of corn and DDGS. Diets were formulated to contain 12% CP and 0.85 Mcal/kg NEg. Heifers were fed treatment diets from 54 d prior to the breeding season through the first trimester. Follicular fluid was collected on day 47 for hormonal analysis. Artificial insemination (AI) was from d 54 to 77. Heifers that did not get pregnant to AI were removed from the study on d 109 and placed with a bull. The study ended on d 180. Data were analyzed using the MIXED procedure of SAS. During the pre-breeding period, algae heifers had lesser DMI (P=0.006) compared to control heifers. Heifers supplemented with algae had greater ADG (P=0.03) during the breeding period and BW tended (P=0.06) to be greater for algae compared to control heifers on d 98 and was greater on d 180 (P=0.03). Dominant follicle diameter and follicular estrogen concentration were unaffected by treatment (P≥0.12). Follicular insulin-like growth factor-1 was greater in algae compared to control heifers (P=0.03). First service conception rate did not differ between treatments (P=0.67); however, second service and overall conception were lesser (P≤0.03) in algae compared to control heifers. These data suggest supplementing DHA-rich algae improved growth but decreased conception rates of primiparous beef females.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"24 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of different culture salinities on the growth and muscle quality of grass carp (Ctenopharyngodon idellus)","authors":"Yongkang Jia, Jia Du, Rujuan Xi, Qi Zhang, Li Li, Dapeng Li, Yasuaki Takagi, Xi Zhang","doi":"10.1093/jas/skae281","DOIUrl":"https://doi.org/10.1093/jas/skae281","url":null,"abstract":"Grass carp (Ctenopharyngodon idellus) is an economically important farmed fish. This experiment was conducted to study the effects of different culture salinities on the growth and muscle quality of grass carp. We found that salinity of 0 – 5 had no significant effect on the growth of grass carp, but it significantly decreased at salinities above 5. Compared to salinity 0, the protein content of serum was significantly higher at salinities of 3 and 5. However, all serum biochemical compound measured, except glucose and total protein, decreased significantly at salinity 9. Muscle textural properties of grass carp were significantly higher at salinities of 3 and 5 than at 0, 7, and 9. Salinities of 0 – 3 had no significant effect on muscle nutrition of grass carp, but this parameter tended to decrease at salinities above 7. Salinities of 3 and 5 significantly favored muscle growth and expression of collagen-related genes, whereas the opposite was true for salinities of 7 and 9. These results indicated that grass carp grew normally at salinities of 3 and 5, with some improvement in muscle quality, whereas salinities of 7 and 9 had a negative effect on growth and quality. Therefore, appropriate salinity levels can help to improve the muscle quality of grass carp without affecting their growth.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"11 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142328643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of increasing levels of salt on intake, digestion, and rumen fermentation with beef cattle consuming low-quality forages","authors":"Hayley C White, Noah G Davis, Megan L Van Emon, Hannah M DelCurto-Wyffels, Samuel A Wyffels, Timothy DelCurto","doi":"10.1093/jas/skae284","DOIUrl":"https://doi.org/10.1093/jas/skae284","url":null,"abstract":"The objectives of this study were to evaluate the levels of supplemental salt on low-quality forage intake, water intake, dry matter digestibility, and rumen fermentation. Six ruminally cannulated, Angus crossbred heifers (14 mo of age; 449 kg ± 24 kg BW) were used in a dual 3 × 3 Latin square design. The heifers were housed in individual stalls with two animals assigned to each treatment per period. Salt treatments were mixed into a protein supplement of 50% cracked corn and 50% soybean meal and fed at 0.3% of shrunk BW. Salt treatments consisted of: 1) control, no salt (CON), 2) 0.05% of BW salt (LOW), and 3) 0.1% of BW salt (HIGH). Chopped, low-quality (CP = 7.4%; NDF = 64.2%), grass hay was used as the base ration and was provided daily at 120% of the average daily intake of the previous 3 days. Each period included a 14-day diet adaptation, 6 days of sample collection, 1 day collection of rumen fluid samples for ruminal and microbial profiles. Individual forage dry matter intake, water intake, and dry matter digestibility were measured during the 6-day collection period. Rumen pH, ammonia levels, and VFA concentrations were measured during the 1-day ruminal profile. Rumen DM and liquid fill were determined with a 5-hour post feeding rumen evacuation. Supplemental salt had no influence on forage intake (P = 0.19) expressed on a kg/day basis yet tended to decrease linearly (P = 0.06) with increasing levels of salt when expressed on a grams/kg body weight basis. Dry matter digestibility was not influenced by salt levels (P > 0.05), but DM fill tended to increase linearly with increasing salt levels (P = 0.06). Water intake and liquid fill, however, increased linearly with increasing level of salt (P < 0.01) with an 18.9% increase in water intake and 17.0% increase in liquid fill compared to control animals. Ruminal pH and ammonia levels both decreased linearly with increasing salt (P < 0.01). Acetate concentration and acetate: propionate ratio increased linearly with increasing levels of salt (P < 0.01). In contrast, isobutyrate and butyrate concentrations decreased linearly with increasing levels of salt (P < 0.01). Our research suggests that increasing levels of salt tends to influence dry matter intake, DM fill, liquid kinetics, and rumen fermentation characteristics. Results from this research provides additional information on how salt-limited supplements may impact beef cattle consuming low-quality forage diets.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"103 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142321498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}