Journal of animal science最新文献

{"title":"Zinc regulation of lipidome remodeling during boar sperm capacitation.","authors":"Ian J Shofner,Kayla Mills,Tyler Weide,Matthew W Breitzman,Karl Kerns","doi":"10.1093/jas/skag009","DOIUrl":"https://doi.org/10.1093/jas/skag009","url":null,"abstract":"Sperm capacitation is essential for fertilization and is characterized by a cascade of biochemical signaling and membrane remodeling events. This process is highly dependent on membrane composition. Profiling lipid alterations provides a critical window into the molecular underpinnings of capacitation and the regulatory influence of zinc ions (Zn2+). Metabolomic studies in boar sperm have shown that capacitation coincides with broad shifts in metabolite abundance and that extracellular zinc attenuates or redirects many of these changes, highlighting its role as a key modulator. To extend this framework to the lipidome, we profiled boar sperm under three conditions: non-capacitated (0 h), capacitated in vitro (4 h), and capacitated with extracellular zinc (4 h + Zn), using liquid chromatography-mass spectrometry and image-based flow cytometry to validate capacitation status. Relative to 0 h, capacitation was associated with altered abundances of 30 lipids (P < 0.05) spanning several lipid categories: fatty acyls (n = 8), sterol lipids (n = 7), sphingolipids (n = 1), glycerolipids (n = 3), glycerophospholipids (n = 4), and unannotated lipids (n = 9). When exogenous Zn2+ was supplemented during in vitro capacitation, 12 of these shifts were maintained at 0 h-like levels (P < 0.05), suggesting an inhibiting or stabilizing role. In 2 of 16 hits, exogenously supplemented Zn2+ enhanced the capacitation-associated change (P < 0.05), whereas in the remaining 14 it exerted no measurable effect (P > 0.05). When exogenous Zn2+ was supplemented during in vitro capacitation, distinct lipid shifts were identified and organized using Tukey's lipid-pattern classification based on significance (P < 0.05) and directionality, organizing them into four categories: Type-1 lipids (capacitation-associated), Type-2 lipids (zinc-inhibited), Type-3 lipids (zinc-specific response), and Type-4 lipids (zinc-enhanced). These categories describe distinct modes of lipid regulation, where some species remained unaffected by zinc (Type-1, n = 16), others were stabilized or inhibited from progressing toward capacitation-associated levels (Type-2, n = 12), a subset responded exclusively to zinc independent of capacitation (Type-3, n = 4), and a small group exhibited amplified capacitation-linked shifts under zinc supplementation (Type-4, n = 2). Together, these data reveal a class-specific, zinc-dependent architecture of lipid remodeling that integrates metabolic and membrane regulation within the broader capacitation cascade.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"4 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145994904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-nutritive sweeteners improve growth, reduce diarrhea, and modulate intestinal and systemic metabolism in weaned pigs.","authors":"Mariah R Jansen,Charlotte Ludorf,Riley E Barber,Veronica I Polniak,Andrea M Luttman,Dale W Rozeboom,Kwangwook Kim","doi":"10.1093/jas/skag005","DOIUrl":"https://doi.org/10.1093/jas/skag005","url":null,"abstract":"Early weaning improves swine productivity but induces stress that impairs growth, compromises intestinal health, and increases diarrhea. A total of 288 weaned pigs (21 ± 1 days; PIC 800 x Yorkshire; initial body weight (BW) 6.21 ± 0.45 kg) were used in a randomized complete block design, with initial BW as the blocking factor and pen as the experimental unit (48 pens; 6 pig/pen). Pigs were assigned to one of four dietary treatments: a nursery basal diet (control; CON), CON supplemented with 150 mg/kg sucralose (SCL). CON supplemented with 30 mg/kg neotame (NEO), or CON supplemented with 50 mg/kg carbadox (CBX). This study investigated the effects of dietary SCL or NEO supplementation on growth performance, diarrhea incidence, immune responses, intestinal development, and serum metabolites in weaned pigs. Pigs supplemented with SCL tended to increase (P = 0.093) average daily gain (ADG) from day 0 to 7 and increased (P < 0.05) average daily feed intake (ADFI) during phase 1 compared with CON. Pigs supplemented with NEO tended to increase (P = 0.083, P = 0.090) BW on days 7 and 14 and ADG (P = 0.053), and increased ADFI (P < 0.05) during phase 1 compared with CON. Both NEO (P < 0.05) and SCL (P < 0.10) reduced diarrhea frequency during phase 1 and across the experimental period compared with CON. SCL improved villus height-to-crypt depth ratio (P < 0.05), and reduced crypt depth (P < 0.05) compared with CON on day 14. SCL downregulated (P < 0.05) tight junction protein 1 (TJP1) expression compared with CBX on day 28. Untargeted serum metabolomics revealed that both SCL and NEO altered amino acid, nucleoside, antioxidant, and lipid metabolic pathways relative to CON. On day 14, SCL altered β-alanine and glutathione metabolism, whereas NEO modulated amino acid-derived metabolites. By day 28, SCL modulated purine, D-amino acid, and ether lipid metabolism, while NEO was associated with taurine and hypotaurine metabolism. These findings indicate that SCL and NEO improve growth performance and reduce post-weaning diarrhea, with SCL additionally enhancing intestinal structure and barrier-related markers, while NEO may act through palatability enhancements or microbiota associated metabolic pathways.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"8 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145968705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overnutrition induced metabolic dysregulation and partially decreased semen quality in young beef bulls.","authors":"Pedro L P Fontes,Dylan B Davis,Lucas Melo-Gonçalves,Samir Burato,Molly S Smith,Alexander Stelzleni,Francis F Fluharty,R Lawton Stewart,Reinaldo F Cooke,John J Bromfield,Alexandra Else-Keller,Karl Kerns,Lew Strickland,Saulo M Zoca","doi":"10.1093/jas/skag004","DOIUrl":"https://doi.org/10.1093/jas/skag004","url":null,"abstract":"The objective of this study was to characterize the systemic metabolic response to overnutrition in young bulls and to evaluate the effects of overnutrition on semen characteristics. Half-sibling yearling beef bulls (n = 44) were utilized in a completely randomized design, where bulls were randomly assigned 1 of 2 dietary treatments (n = 4 pens/treatment): 1) Moderate Gain (MG): diet formulated to promote an average daily gain of 1.2 kg/day, or 2) High Gain (HG): diet formulated to promote an average daily gain of 1.8 kg/day. Bulls were housed in a feedlot facility equipped with an automated individual feed intake monitoring system and fed their respective diets for 114 days. Body weight, carcass ultrasonography, and blood samples were collected on days 0, 36, 76, and 114. Blood samples were utilized to determine circulating concentrations of cholesterol, triglycerides, glucose, insulin, leptin, low-density lipoprotein, non-esterified fatty acids (NEFA), beta-hydroxybutyrate (BHB), testosterone, and haptoglobin. Serial semen samples were collected at the end of the feeding period (days 109, 111, and 114) and analyzed using computer assisted sperm analysis and image-based flow cytometry. Body weight and subcutaneous backfat thickness were greater (P < 0.01) at the end of the feeding period in HG compared with MG bulls. Similar results were observed for circulating concentrations of glucose, insulin, leptin, cholesterol, and low-density lipoproteins (P ≤ 0.02). Alternatively, circulating concentrations of NEFA and BHB were decreased (P < 0.01) in HG bulls at the end of the feeding period. Bulls exposed to the HG diet had greater (P < 0.01) insulin resistance at the end of the feeding period based on insulin: glucose ratio and revised quantitative insulin sensitivity check index (RQUICKI). HG bulls had greater (P = 0.02) plasma haptoglobin compared with MG bulls, whereas testosterone concentrations were similar (P = 0.69). Bulls exposed to the HG diet tended to have decreased (P ≤ 0.09) total and progressive motility compared with MG bulls. Moreover, the proportion of sperm with partially damaged acrosome tended (P = 0.09) to be increased and the proportion of sperm with intact plasma membrane tended to be reduced (P ≤ 0.10) in HG bulls compared with MG bulls. In summary, HG dietary treatment promoted an obese-like metabolic profile that increased insulin resistance, circulating haptoglobin, and resulted in a subtle decrease in semen quality.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"250 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145956201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nontargeted plasma metabolomics associated with sow lifetime productivity traits.","authors":"L A Rempel,D J Nonneman","doi":"10.1093/jas/skag003","DOIUrl":"https://doi.org/10.1093/jas/skag003","url":null,"abstract":"The current study was conducted to characterize metabolomic plasma profiles among sows differing in their lifetime born alive and lifetime weaned after four parities. Plasma samples were collected at harvest between 12-15 days (luteal phase) following their fourth parity post-weaning estrus from 120 dams with consistent born alive and weaned at every farrowing event. Categories were derived as follows for average lifetime born alive (ba): High (H; 61ba), Mid (M; 50ba), and Low (L; 39ba) and raised (wn): High (H; 50wn) and Low (L; 34wn) generating 6 categories with 20 dams in each: HH, HL, MH, ML, LH, and LL, respective to born alive: raised. Plasma samples were submitted for ultra-performance liquid chromatography (positive and negative ionization modes) - mass spectrometry (UPLC-MS) to isolate putative compounds. Analysis of variance with a false discovery correction was performed to determine categorical differences of putative compounds. Negative mode ionization UPLC-MS yielded 92 compounds different (P < 0.05) by category, while positive ionization mode provided 644 compounds different (P < 0.05) by category. Twenty-five putative compounds were different (P < 0.05) for the LL and ML categories versus HH and MH categories. A putative unique secondary bacterial compound structurally similar to saponins, MK-800-62F1, and an annotated fatty acid, lignoceroylsphingosine, were increased (P < 0.05) in HH dams. Several possible fatty acid, eicosanoid, and steroid compounds had greater (P < 0.05) intensities in LL and LH dams. Several annotated eicosanoid compounds, leukotriene B4, 5(S)-HETE, 15(S)-Hpete and a PGF1α product, can be biosynthesized in blood in response to an inflammatory stimulus. Probable steroid compounds had increased signal intensity in plasma from LL and LH dams, including neuroactive steroids such as corticosteroids and allopregnanolone and suggested derivatives of testosterone and progesterone steroid compounds. Characterization of plasma profiles among post fourth parity dams with differing lifetime born alive and weaned production traits suggested unique features that may be related to various physiological systems including immunological, metabolic, and hormonal. Future work verifying compounds and validating in adolescent females may provide suitable predictors of lifetime production traits.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"5 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145937792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of pre-transit zinc supplementation and transit duration on performance, metabolites, and feeding behavior of beef steers.","authors":"Allison M Baumhover,Brock M Ortner,Daniel U Thomson,Karen S Schwartzkopf-Genswein,Stephanie L Hansen","doi":"10.1093/jas/skag002","DOIUrl":"https://doi.org/10.1093/jas/skag002","url":null,"abstract":"Effects of pre-transit zinc (Zn) supplementation and transit duration on performance, metabolites, and feeding behavior were assessed using 80 Angus-crossbred steers in a 2 × 2 factorial design. Pre-transit diets (DIET) of no supplemental Zn (Zn0; analyzed at 40 mg Zn/kg DM) or 100 mg Zn/kg DM supplemental Zn from ZnSO4 (Zn100) were fed for 42 days. Cattle were assigned to an 8 (8H) or 18 hours (18H) transit duration (DUR). All cattle received Zn100 diet for 56 days post-transit. Steers were stratified by bodyweight (BW) to pens (5 steers/pen) equipped with a GrowSafe® feeding behavior and intake system (Vytelle, Ames, IA USA). Weights were collected on d -42, -41, -1, 0, 1, 2, 7, 28, 55, and 56 relative to transit; blood was collected on d -42, -1, 1, 2, and 7. GrowSafe® feeding behavior data were collected continuously for 7d pre- and post-transit. Data were analyzed using the Mixed Procedure of SAS with DIET, DUR, and DIET×DUR as fixed effects. Feeding behavior, metabolites and weekly dry matter intakes (DMI) were analyzed as repeated measures with the repeated effect hour, day, or week. Pre-transit average daily gain (ADG), gain: feed (G: F), d -1 BW (DIET P ≤ 0.03) and DMI on weeks 2 and 6 (DIET×Week P = 0.01) were greater for the Zn100 cattle. Shrink was 7.3% for 18H and 5.2% 8H (DUR P < 0.01). Immediately post-transport, 8H cattle had greater serum glucose and insulin, but lesser non-esterified fatty acids (NEFA) than 18H (DUR P ≤ 0.05) cattle. On d 2, 8H had lesser glucose and NEFA than 18H (DUR P ≤ 0.01), and insulin was not different (DUR P = 0.17). Zn0-18H was the most insulin sensitive on d 1 (DIET×DUR P = 0.01), while 8H was more sensitive than 18H (DUR P = 0.01) on d 2. Feeding duration and frequency were affected by DIET×DUR×Day (P < 0.01). The rate of intake was greater for 8H than 18H at 24, but lesser at 48 and 72 h post-transit (DUR P < 0.01). Time spent at the bunk with head down was greater for 8H at 24, 48, 72, and 144 h post -transport than 18H cattle (DUR P < 0.01). Zn0 cattle had greater post-transit G: F than Zn100 group (DIET P < 0.01). On wk 7, Zn100 had greater weekly DMI, but lesser on wk 12 (DIET P = 0.01). By study's end, performance was not different between treatments (P ≥ 0.11). These data suggest a benefit for Zn supplementation prior to transit on performance, with minimal impacts of transit duration. However, in the short-term, transit duration altered metabolic demand and feeding behavior of beef cattle.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"49 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145937794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling the bacteriome of a diet fed in meal or pelleted form, delivered as dry, wet/dry or liquid feed and its impact on the fecal and intestinal bacteriome of grow-finisher pigs.","authors":"James T Cullen,Peadar G Lawlor,Paul Cormican,Gillian E Gardiner","doi":"10.1093/jas/skaf461","DOIUrl":"https://doi.org/10.1093/jas/skaf461","url":null,"abstract":"Research is limited on how feed-associated microbes impact the intestinal bacteriome, growth and feed efficiency of pigs. The aims of this study were to; (1) profile the bacteriome of a meal or pelleted diet, delivered as dry, wet/dry or liquid feed using 16S rRNA gene sequencing; (2) determine its impact on the fecal and intestinal bacteriome of grow-finisher pigs; (3) investigate if differentially abundant bacterial taxa are correlated with growth parameters of these pigs. The experiment was a 2 x 3 factorial arrangement, with two factors for feed form (meal, pellets) and three factors for feed delivery (dry, liquid, wet/dry). It involved 216 Danavil Duroc x (Large White x Landrace) pigs penned in same sex pen groups of 6 pigs of similar weight (average ∼33.3 kg). Pen groups were blocked by sex and weight before being randomly assigned to 1 of 6 wheat-barley-soya-based dietary treatments in a completely randomised block design: (1) Dry meal; (2) Dry pellets; (3) Liquid meal; (4) Liquid pellets; (5) Wet/dry meal; (6) Wet/dry pellets. Diets were fed on an ad-libitum basis for 64 days. Liquid feed was prepared at a water: feed ratio of 2.5:1 (fresh matter basis). Dry feed was sampled from silos and bags and liquid feed from mixing tanks and troughs. Bacterial richness was lower in the dry pellets compared to meal (P ≤ 0.05). The liquid feed bacteriome was more diverse than that of dry feed (P ≤ 0.001). Weissella and Leuconostoc had higher relative abundance (RA) in residual-trough sampled liquid feed compared to mixing tank and fresh trough-sampled feed. The ileal bacteriome was more diverse (P ≤ 0.01) in meal-fed than pellet-fed pigs, with higher RA of Megasphaera and Mitsuokella, while Streptococcus and Escherichia-Shigella had greater RA in pellet-fed pigs (P ≤ 0.01). Lactobacillus was enriched in the intestinal digesta of liquid meal-fed pigs (P ≤ 0.05), corresponding with its predominance in this diet. Liquid meal-, liquid pellet- and wet/dry pellet-fed pigs had the highest average daily gain (P < 0.001). Feed conversion efficiency (FCE) was better in dry pellet-fed compared to liquid-fed pigs (P < 0.001). Leuconostoc (associated with feed fermentation) was most abundant in the feces and ileal digesta of liquid-fed pigs and correlated with poorer FCE (P ≤ 0.05). The same Leuconostoc found in liquid feed were also detected in the digesta and feces of liquid-fed pigs, implicating feed bacteria as a potential cause of the poorer FCE of liquid-fed pigs.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"47 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145937790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of the Organization and Complexity of Immunoglobulin Light Chain Loci in Equids.","authors":"Yanbo Qiu,Yichen Lei,Xiaohua Yi,Xiaoqin Tang,Beibei Zhang,Shuhui Wang,Xiuzhu Sun","doi":"10.1093/jas/skag001","DOIUrl":"https://doi.org/10.1093/jas/skag001","url":null,"abstract":"This study investigated three donkey breeds-Guanzhong, Jiami, and Northern Shaanxi-to characterize the structural organization and diversification mechanisms of the immunoglobulin light-chain (IgL) loci, and to conduct both intra-breed and interspecies comparisons with horses. The donkey IGλ locus is located on chromosome 8 and arranged in a Vλ-(Jλ-Cλ)-Vλ configuration. It contains 7 Cλ genes, each preceded by a corresponding Jλ gene to form a Jλ-Cλ cluster. Upstream of this cluster, 156 Vλ genes were identified, including 29 potential functional genes; downstream, 98 Vλ genes were detected, of which 22 were potentially functional. The IGκ locus resides on chromosome 6 and exhibits a Vκ-Jκ-Cκ structure, comprising one Cκ gene, 5 Jκ genes, and 72 Vκ segments, including 22 potential functional Vκ genes. Expression profiling revealed clear light-chain usage preferences in donkeys. For the λ-chain, Vλ72, Vλ135, Vλ150, Vλ190, and Vλ196-mainly belonging to the IGLV1 and IGLV3 subgroups-were preferentially utilized. For the κ-chain, Vκ67 and Vκ71 were dominantly expressed, highlighting the prominent role of the IGκV4 subgroup. Horses showed a similar pattern, with strong biases toward the IGLV1, IGLV3, and IGκV4 subgroups. Although breed-specific differences were observed in certain IGL expression profiles (e.g. V-J combinations), these variations were largely restricted to the λ-chain and involved low-frequency genes. Thus, highly utilized genes and subgroups exhibit high conservation among donkey breeds. This study is the first to comprehensively elucidate the structure and expression preferences of the donkey IgL locus, laying a solid foundation for the development of donkey-derived antibody resources.","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"146 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145937791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Technologies and opportunities to improve sustainability of swine production systems.","authors":"Laura Greiner","doi":"10.1093/jas/skaf427","DOIUrl":"10.1093/jas/skaf427","url":null,"abstract":"","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":"104 ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12782652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ASAS-NANP symposium: mathematical modeling in animal nutrition: training the future generation in data and predictive analytics for sustainable development. A summary of the 2024 symposium.","authors":"Luis O Tedeschi, Hector M Menendez Iii","doi":"10.1093/jas/skaf452","DOIUrl":"10.1093/jas/skaf452","url":null,"abstract":"","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12873758/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145843725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of bovine respiratory disease on tissue-specific regulation of Zn and vitamin a metabolism and apparent absorption and retention of trace minerals.","authors":"Emma L Rients, Stephanie L Hansen, Jodi L Mcgill","doi":"10.1093/jas/skaf453","DOIUrl":"10.1093/jas/skaf453","url":null,"abstract":"<p><p>This study aimed to characterize trace mineral and vitamin A metabolism and redistribution during clinical and subclinical respiratory infection in beef on dairy crossbred steers (n = 29; BW = 230 ± 2.14 kg). Steers were assigned to one of four groups encompassing days -6 to -1, 0 to 5, 5 to 10, and 10 to 15 of an experimental viral-bacterial respiratory challenge. Steers were adapted to metabolism crates for 5 d prior to a 5-d total urine and fecal collection period and necropsied at the end of the period. On day 0, steers were inoculated with bovine respiratory syncytial virus strain 375 followed by an intratracheal inoculation with Mannheimia haemolytica strain D153 on day 7. A natural disease challenge occurred during the study, leading to all steers showing signs of disease at necropsy. Lung pathology scores, plasma Fe concentrations, and rectal temperatures for 5 d prior to necropsy were used to categorize animals into clinical (n = 9) and subclinical (n = 20) disease. These categories were confirmed by decreases in dry matter intake (P = 0.06) and nitrogen retention (P = 0.06) in animals with clinical disease compared to subclinical. Plasma concentrations of Zn and retinol were lesser in clinical disease (P ≤ 0.005). Conversely, liver (P = 0.02) and kidney (P = 0.06) concentrations of Zn were higher in clinical disease. This tissue sequestration occurred despite no difference in apparent Zn absorption or retention (P ≥ 0.69), providing evidence of systemic mineral redistribution. There was also no difference in the apparent absorption of Cu, Fe, and Mn (P ≥ 0.44), despite some differences in tissue concentrations. At the site of infection, expression of genes regulating vitamin A transport and metabolism (STRA6, RXRα, RBP4) increased (P ≤ 0.002) in non-lesion lung relative to diseased lung. In both lesion and non-lesion lung, clinical disease decreased RALDH2 expression relative to subclinical disease (P = 0.05). These findings demonstrate that BRD induces a coordinated redistribution of trace minerals from circulation to key tissues and alters local vitamin A metabolism in the lung. This highlights that plasma micronutrient concentrations during infection are not reflective of total body status, but rather an organized physiological response that prioritizes tissue-level demands.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145878325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}