The objective of this study was to evaluate the effects of dietary fatty acids (FA) saturation and lysophospholipids supplementation on growth, meat quality, oxidative stability, FA profiles, and lipid metabolism of finishing beef bulls. Thirty-two Angus bulls (initial body weight: 623 ± 22.6 kg; 21 ± 0.5 months of age) were used. The experiment was a completely randomized block design with a 2 × 2 factorial arrangement of treatments: 2 diets with FA of different degree of unsaturation [high saturated FA diet (HSFA) vs. high unsaturated FA diet (HUFA)] combined with (0.075%, dry matter basis) and without lysophospholipids supplementation. The bulls were fed a high-concentrate diet (forage to concentrate, 15:85) for 104 d including a 14-d adaptation period and a 90-d data and sample collection period. No interactions were observed between dietary FA and lysophospholipids supplementation for growth and meat quality parameters. A greater dietary ratio of unsaturated FA (UFA) to saturated FA (SFA) from 1:2 to 1:1 led to lower DM intake and backfat thickness, but did not affect growth performance and other carcass traits. Compared with HSFA, bulls fed HUFA had greater shear force in Longissimus thoracis (LT) muscle, but had lower intramuscular fat (IMF) content and SOD content in LT muscle. Compared with HUFA, feeding the HSFA diet up-regulated expression of ACC, FAS, PPARγ, and SCD1, but down-regulated expression of CPT1B. Compared with feeding HSFA, the HUFA diet led to greater concentrations of c9-C18:1 and other monounsaturated FA in LT muscle. Feeding HUFA also led to lower plasma concentrations of cholesterol, but there were no interactions between FA and lysophospholipids detected. Feeding lysophospholipids improved growth and feed conversion ratio and altered meat quality by increasing muscle pH24h, redness values (24 h), IMF content, and concentrations of C18:3, C20:5 and total polyunsaturated fatty acids. Furthermore, lysophospholipids supplementation led to lower malondialdehyde content and up-regulated the expression of ACC, FAS, and LPL in LT muscle. Results indicated that supplementing a high-concentrate diet with lysophospholipids to beef bulls can enhance growth rate, feed efficiency, meat quality, and beneficial FA. Increasing the dietary ratio of UFA to SFA reduced DM intake and backfat thickness without compromising growth, suggesting potential improvements in feed efficiency.
{"title":"Impact of dietary lysophospholipids supplementation on growth performance, meat quality, and lipid metabolism in finishing bulls fed diets varying in fatty acid saturation","authors":"Meimei Zhang, Haixin Bai, Ruixue Wang, Yufan Zhao, Wenzhu Yang, Jincheng Liu, Yonggen Zhang, Peixin Jiao","doi":"10.1186/s40104-024-01138-w","DOIUrl":"https://doi.org/10.1186/s40104-024-01138-w","url":null,"abstract":"The objective of this study was to evaluate the effects of dietary fatty acids (FA) saturation and lysophospholipids supplementation on growth, meat quality, oxidative stability, FA profiles, and lipid metabolism of finishing beef bulls. Thirty-two Angus bulls (initial body weight: 623 ± 22.6 kg; 21 ± 0.5 months of age) were used. The experiment was a completely randomized block design with a 2 × 2 factorial arrangement of treatments: 2 diets with FA of different degree of unsaturation [high saturated FA diet (HSFA) vs. high unsaturated FA diet (HUFA)] combined with (0.075%, dry matter basis) and without lysophospholipids supplementation. The bulls were fed a high-concentrate diet (forage to concentrate, 15:85) for 104 d including a 14-d adaptation period and a 90-d data and sample collection period. No interactions were observed between dietary FA and lysophospholipids supplementation for growth and meat quality parameters. A greater dietary ratio of unsaturated FA (UFA) to saturated FA (SFA) from 1:2 to 1:1 led to lower DM intake and backfat thickness, but did not affect growth performance and other carcass traits. Compared with HSFA, bulls fed HUFA had greater shear force in Longissimus thoracis (LT) muscle, but had lower intramuscular fat (IMF) content and SOD content in LT muscle. Compared with HUFA, feeding the HSFA diet up-regulated expression of ACC, FAS, PPARγ, and SCD1, but down-regulated expression of CPT1B. Compared with feeding HSFA, the HUFA diet led to greater concentrations of c9-C18:1 and other monounsaturated FA in LT muscle. Feeding HUFA also led to lower plasma concentrations of cholesterol, but there were no interactions between FA and lysophospholipids detected. Feeding lysophospholipids improved growth and feed conversion ratio and altered meat quality by increasing muscle pH24h, redness values (24 h), IMF content, and concentrations of C18:3, C20:5 and total polyunsaturated fatty acids. Furthermore, lysophospholipids supplementation led to lower malondialdehyde content and up-regulated the expression of ACC, FAS, and LPL in LT muscle. Results indicated that supplementing a high-concentrate diet with lysophospholipids to beef bulls can enhance growth rate, feed efficiency, meat quality, and beneficial FA. Increasing the dietary ratio of UFA to SFA reduced DM intake and backfat thickness without compromising growth, suggesting potential improvements in feed efficiency.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"13 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142937088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-07DOI: 10.1186/s40104-024-01140-2
Ziyu Liu, Tsungcheng Tsai, Bin Zuo, Samantha Howe, Jason E. Farrar, Christopher E. Randolph, Charles V. Maxwell, Jiangchao Zhao
Sow longevity and reproductivity are essential in the modern swine industry. Although many studies have focused on the genetic and genomic factors for selection, little is known about the associations between the microbiome and sows with longevity in reproduction. In this study, we collected and sequenced rectal and vaginal swabs from 48 sows, nine of which completed up to four parities (U4P group), exhibiting reproductive longevity. We first identified predictors of sow longevity in the rectum (e.g., Akkermansia) and vagina (e.g., Lactobacillus) of the U4P group using RandomForest in the early breeding stage of the first parity. Interestingly, these bacteria in the U4P group showed decreased predicted KEGG gene abundance involved in the biosynthesis of amino acids. Then, we tracked the longitudinal changes of the microbiome over four parities in the U4P sows. LEfSe analysis revealed parity-associated bacteria that existed in both the rectum and vagina (e.g., Streptococcus in Parity 1, Lactobacillus in Parity 2, Veillonella in Parity 4). We also identified patterns of bacterial change between the early breeding stage (d 0) and d 110, such as Streptococcus, which was decreased in all four parties. Furthermore, sows in the U4P group with longevity potential also showed better reproductive performance. Finally, we discovered bacterial predictors (e.g., Prevotellaceae NK3B31 group) for the total number of piglets born throughout the four parities in both the rectum and vagina. This study highlights how the rectal and vaginal microbiome in sows with longevity in reproduction changes within four parities. The identification of parity-associated, pregnancy-related, and reproductive performance-correlated bacteria provides the foundation for targeted microbiome modulation to improve animal production.
{"title":"The sow vaginal and gut microbiota associated with longevity and reproductive performance","authors":"Ziyu Liu, Tsungcheng Tsai, Bin Zuo, Samantha Howe, Jason E. Farrar, Christopher E. Randolph, Charles V. Maxwell, Jiangchao Zhao","doi":"10.1186/s40104-024-01140-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01140-2","url":null,"abstract":"Sow longevity and reproductivity are essential in the modern swine industry. Although many studies have focused on the genetic and genomic factors for selection, little is known about the associations between the microbiome and sows with longevity in reproduction. In this study, we collected and sequenced rectal and vaginal swabs from 48 sows, nine of which completed up to four parities (U4P group), exhibiting reproductive longevity. We first identified predictors of sow longevity in the rectum (e.g., Akkermansia) and vagina (e.g., Lactobacillus) of the U4P group using RandomForest in the early breeding stage of the first parity. Interestingly, these bacteria in the U4P group showed decreased predicted KEGG gene abundance involved in the biosynthesis of amino acids. Then, we tracked the longitudinal changes of the microbiome over four parities in the U4P sows. LEfSe analysis revealed parity-associated bacteria that existed in both the rectum and vagina (e.g., Streptococcus in Parity 1, Lactobacillus in Parity 2, Veillonella in Parity 4). We also identified patterns of bacterial change between the early breeding stage (d 0) and d 110, such as Streptococcus, which was decreased in all four parties. Furthermore, sows in the U4P group with longevity potential also showed better reproductive performance. Finally, we discovered bacterial predictors (e.g., Prevotellaceae NK3B31 group) for the total number of piglets born throughout the four parities in both the rectum and vagina. This study highlights how the rectal and vaginal microbiome in sows with longevity in reproduction changes within four parities. The identification of parity-associated, pregnancy-related, and reproductive performance-correlated bacteria provides the foundation for targeted microbiome modulation to improve animal production.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"36 3 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-06DOI: 10.1186/s40104-024-01135-z
Makenzie G. Newton, Arianna N. Lopez, Claire Stenhouse, Karina L. Hissen, Erin D. Connolly, Xingchi Li, Lan Zhou, Guoyao Wu, William B. Foxworth, Fuller W. Bazer
Meat goat production is a worldwide industry with products such as meat, milk, soap, and fiber being produced. There are approximately 2.6 million meat goats in the United States. For breeding female ruminants, early pregnancy loss is estimated to be 30% within the first month of gestation. Extracellular L-citrulline (a precursor to L-arginine) is not degraded by ruminal microbes due to the lack of uptake. L-Arginine and thus L-citrulline, have beneficial impacts on placentation and, subsequently, fetal-placental development and survival. This study aimed to determine the impact of feeding L-citrulline to meat goats during gestation to improve reproductive success. Meat goats were fed either a control (CON) or L-citrulline (CIT) supplemented diet from d 12 to 82 of gestation. Blood samples were collected and sera were subjected to high-performance liquid chromatography analyses to quantify the abundance of amino acids. Pregnancy rates were determined on d 30, 61, and 90 of gestation, and litter weight, individual birth weights, and 90 d adjusted weaning weights were collected. The concentrations of citrulline, ornithine, and arginine were greater in CIT does compared to CON does, but there was no difference in pregnancy rates between CON and CIT does. Birth weight was greater for male kids born as singles when compared to females, but this phenotype was not observed for kids born as twins or triplets. Further, males born to CON does had greater 90 d adjusted weaning weights than females, but this was not observed in the CIT group. Female kids born to CON and CIT Boer goats had heavier 90 d adjusted weaning weights than those born to Spanish or F1 Boer-Spanish does. This study provides proof of concept that feeding dietary L-citrulline increases concentrations of citrulline and arginine in blood of gestating meat goats. However, further studies are needed to understand the cellular mechanisms impacted by feeding this supplement. Regardless, this study demonstrated that feeding L-citrulline has the potential to increase reproductive performance in gestating ruminants.
{"title":"Impact of dietary supplementation of L-citrulline to meat goats during gestation on reproductive performance","authors":"Makenzie G. Newton, Arianna N. Lopez, Claire Stenhouse, Karina L. Hissen, Erin D. Connolly, Xingchi Li, Lan Zhou, Guoyao Wu, William B. Foxworth, Fuller W. Bazer","doi":"10.1186/s40104-024-01135-z","DOIUrl":"https://doi.org/10.1186/s40104-024-01135-z","url":null,"abstract":"Meat goat production is a worldwide industry with products such as meat, milk, soap, and fiber being produced. There are approximately 2.6 million meat goats in the United States. For breeding female ruminants, early pregnancy loss is estimated to be 30% within the first month of gestation. Extracellular L-citrulline (a precursor to L-arginine) is not degraded by ruminal microbes due to the lack of uptake. L-Arginine and thus L-citrulline, have beneficial impacts on placentation and, subsequently, fetal-placental development and survival. This study aimed to determine the impact of feeding L-citrulline to meat goats during gestation to improve reproductive success. Meat goats were fed either a control (CON) or L-citrulline (CIT) supplemented diet from d 12 to 82 of gestation. Blood samples were collected and sera were subjected to high-performance liquid chromatography analyses to quantify the abundance of amino acids. Pregnancy rates were determined on d 30, 61, and 90 of gestation, and litter weight, individual birth weights, and 90 d adjusted weaning weights were collected. The concentrations of citrulline, ornithine, and arginine were greater in CIT does compared to CON does, but there was no difference in pregnancy rates between CON and CIT does. Birth weight was greater for male kids born as singles when compared to females, but this phenotype was not observed for kids born as twins or triplets. Further, males born to CON does had greater 90 d adjusted weaning weights than females, but this was not observed in the CIT group. Female kids born to CON and CIT Boer goats had heavier 90 d adjusted weaning weights than those born to Spanish or F1 Boer-Spanish does. This study provides proof of concept that feeding dietary L-citrulline increases concentrations of citrulline and arginine in blood of gestating meat goats. However, further studies are needed to understand the cellular mechanisms impacted by feeding this supplement. Regardless, this study demonstrated that feeding L-citrulline has the potential to increase reproductive performance in gestating ruminants.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-05DOI: 10.1186/s40104-024-01136-y
Xiaodong Su, Lei Zhang, Yiyang Sun, Yanbo Wu, Jianrong Ren, Shengru Wu, Xinjian Lei, Jun Zhang, Dangdang Wang, Hao Ren, Junhu Yao
The diverse types and processing methods of grains intricately influence the sites and digestibility of starch digestion, thereby impacting energy utilization. This study aimed to explore the impact of grain variety and processing methods on the net energy (NE) in dairy goats, analyzing these effects at the level of nutrient digestion and metabolism. Eighteen castrated Guanzhong dairy goats (44.25 ± 3.59 kg BW) were randomly divided into 3 groups, each consisting of 6 replicates. The substitution method was employed to determine the NE values of the dry-rolled corn (DRC), dry-rolled wheat (DRW) or steam-flaked corn (SFC, 360 g/L). Briefly, two phases were performed. Throughout the basal phase, all goats were fed the same basal diet. In the substitution phase, 30% of the basal diet was replaced with DRC, DRW and SFC, respectively. In this study, the NE values of the DRC, DRW and SFC were 7.65, 7.54 and 7.44 MJ/kg DM, respectively. Compared to the DRC group, the DRW group showed increased digestibility of starch and crude protein (CP). Similarly, the SFC group exhibited increased organic matter (OM) and starch digestibility and a trend towards higher dry matter (DM) digestibility, reduced fecal OM and starch content. Additionally, fecal volatile fatty acid (VFA) concentrations decreased in goats fed SFC. Correspondingly, digestible energy (DE) in the DRW and SFC groups tended to be higher than in the DRC group. DRW increased total VFA concentration compared to DRC, while SFC increased the proportion of propionate and decreased the acetate-to-propionate ratio in the rumen. Both the DRW and SFC diets elevated serum glucose levels. Furthermore, heat increment (HI) and gaseous energy (GasE) related to fermentation were significantly higher in the DRW and SFC groups compared to the DRC group. Our findings indicated that DRW and SFC increased rumen starch fermentation in goats, thereby improving total tract starch digestion and DE. However, DRW and SFC failed to improve NE value due to increased heat and gas energy production from fermentation. Therefore, excessively refined grains processing in the diet of dairy goats does not effectively improve energy efficiency.
不同的谷物种类和加工方式错综复杂地影响着淀粉消化的部位和消化率，从而影响能量的利用。本研究旨在探讨籽粒品种和加工方式对奶山羊净能（NE）的影响，并在营养物质消化代谢水平上分析这些影响。选取18只去势（44.25±3.59 kg BW）的关中奶山羊，随机分为3组，每组6个重复。采用代入法测定干轧玉米（DRC）、干轧小麦（DRW）和蒸片玉米（SFC, 360 g/L）的NE值。简单地说，进行了两个阶段。在整个基础阶段，所有山羊均饲喂相同的基础饲粮。在替代阶段，分别用DRC、DRW和SFC替代30%的基础饲粮。本研究中，DRC、DRW和SFC的NE值分别为7.65、7.54和7.44 MJ/kg DM。与DRC组相比，DRW组的淀粉和粗蛋白质（CP）消化率均有所提高。同样，SFC组表现出有机物和淀粉消化率升高，干物质消化率升高，粪便有机质和淀粉含量降低的趋势。此外，SFC组山羊的粪便挥发性脂肪酸（VFA）浓度降低，相应的，DRW组和SFC组的消化能（DE）趋于高于DRC组。与DRC相比，DRW增加了瘤胃总VFA浓度，而SFC增加了瘤胃丙酸比例，降低了瘤胃乙酸/丙酸比。DRW和SFC饮食均能提高血清葡萄糖水平。此外，DRW和SFC组与发酵相关的热增量（HI）和气态能（GasE）显著高于DRC组。本研究结果表明，DRW和SFC提高了山羊瘤胃淀粉发酵，从而提高了全肠道淀粉消化和消化能，但由于发酵产生的热量和气体能量增加，DRW和SFC未能提高NE值。因此，在奶山羊日粮中过度加工精粮并不能有效提高能量利用效率。
{"title":"Net energy of grains for dairy goats differed with processing methods and grain types","authors":"Xiaodong Su, Lei Zhang, Yiyang Sun, Yanbo Wu, Jianrong Ren, Shengru Wu, Xinjian Lei, Jun Zhang, Dangdang Wang, Hao Ren, Junhu Yao","doi":"10.1186/s40104-024-01136-y","DOIUrl":"https://doi.org/10.1186/s40104-024-01136-y","url":null,"abstract":"The diverse types and processing methods of grains intricately influence the sites and digestibility of starch digestion, thereby impacting energy utilization. This study aimed to explore the impact of grain variety and processing methods on the net energy (NE) in dairy goats, analyzing these effects at the level of nutrient digestion and metabolism. Eighteen castrated Guanzhong dairy goats (44.25 ± 3.59 kg BW) were randomly divided into 3 groups, each consisting of 6 replicates. The substitution method was employed to determine the NE values of the dry-rolled corn (DRC), dry-rolled wheat (DRW) or steam-flaked corn (SFC, 360 g/L). Briefly, two phases were performed. Throughout the basal phase, all goats were fed the same basal diet. In the substitution phase, 30% of the basal diet was replaced with DRC, DRW and SFC, respectively. In this study, the NE values of the DRC, DRW and SFC were 7.65, 7.54 and 7.44 MJ/kg DM, respectively. Compared to the DRC group, the DRW group showed increased digestibility of starch and crude protein (CP). Similarly, the SFC group exhibited increased organic matter (OM) and starch digestibility and a trend towards higher dry matter (DM) digestibility, reduced fecal OM and starch content. Additionally, fecal volatile fatty acid (VFA) concentrations decreased in goats fed SFC. Correspondingly, digestible energy (DE) in the DRW and SFC groups tended to be higher than in the DRC group. DRW increased total VFA concentration compared to DRC, while SFC increased the proportion of propionate and decreased the acetate-to-propionate ratio in the rumen. Both the DRW and SFC diets elevated serum glucose levels. Furthermore, heat increment (HI) and gaseous energy (GasE) related to fermentation were significantly higher in the DRW and SFC groups compared to the DRC group. Our findings indicated that DRW and SFC increased rumen starch fermentation in goats, thereby improving total tract starch digestion and DE. However, DRW and SFC failed to improve NE value due to increased heat and gas energy production from fermentation. Therefore, excessively refined grains processing in the diet of dairy goats does not effectively improve energy efficiency.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"37 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-04DOI: 10.1186/s40104-024-01137-x
Xue Qin, Menghao Yang, Yang Yu, Xiaolin Wang, Yi Zheng, Rui Cai, Weijun Pang
Increased backfat thickness of sows in early gestation is negative to reproductive performance. Endometrial receptivity is an important determinant of reproductive success, but it is unclear whether the effect of sow backfat thickness on litter size is associated with endometrial receptivity and whether melatonin treatment may have benefits. The present study seeks to answer these questions through in vitro and in vivo investigations. Excessive lipid deposition and lower melatonin levels in the uterus are detrimental to endometrial receptivity and embryo implantation in high backfat thickness sows. In cells treated with melatonin, the MT2/PI3K/LIF axis played a role in reducing lipid accumulation in porcine endometrial epithelium cells and improved endometrial receptivity. Furthermore, we found a reduction of lipids in the uterus after eight weeks of intraperitoneal administration of melatonin to HFD mice. Notably, melatonin treatment caused a significant reduction in the deposition of endometrial collagen, an increase in the number of glands, and repair of the pinopode structure, ultimately improving endometrial receptivity, promoting embryo implantation, and increasing the number of litter size of mice. Collectively, the finding reveals the harmful effects of high backfat thickness sows on embryo implantation and highlight the role of melatonin and the MT2/PI3K/LIF axis in improving endometrial receptivity by enhancing metabolism and reducing the levels of uterine lipids in obese animals.
{"title":"Melatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows","authors":"Xue Qin, Menghao Yang, Yang Yu, Xiaolin Wang, Yi Zheng, Rui Cai, Weijun Pang","doi":"10.1186/s40104-024-01137-x","DOIUrl":"https://doi.org/10.1186/s40104-024-01137-x","url":null,"abstract":"Increased backfat thickness of sows in early gestation is negative to reproductive performance. Endometrial receptivity is an important determinant of reproductive success, but it is unclear whether the effect of sow backfat thickness on litter size is associated with endometrial receptivity and whether melatonin treatment may have benefits. The present study seeks to answer these questions through in vitro and in vivo investigations. Excessive lipid deposition and lower melatonin levels in the uterus are detrimental to endometrial receptivity and embryo implantation in high backfat thickness sows. In cells treated with melatonin, the MT2/PI3K/LIF axis played a role in reducing lipid accumulation in porcine endometrial epithelium cells and improved endometrial receptivity. Furthermore, we found a reduction of lipids in the uterus after eight weeks of intraperitoneal administration of melatonin to HFD mice. Notably, melatonin treatment caused a significant reduction in the deposition of endometrial collagen, an increase in the number of glands, and repair of the pinopode structure, ultimately improving endometrial receptivity, promoting embryo implantation, and increasing the number of litter size of mice. Collectively, the finding reveals the harmful effects of high backfat thickness sows on embryo implantation and highlight the role of melatonin and the MT2/PI3K/LIF axis in improving endometrial receptivity by enhancing metabolism and reducing the levels of uterine lipids in obese animals.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"7 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142924988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-03DOI: 10.1186/s40104-024-01132-2
Qianyun Zhang, Qidong Zhu, Yunqi Xiao, Qinghua Yu, Shourong Shi
Salmonella enterica serovar Enteritidis (S. Enteritidis) is a global foodborne pathogen that poses a significant threat to human health, with poultry being the primary reservoir host. Therefore, addressing S. Enteritidis infections in poultry is crucial to protect human health and the poultry industry. In this study, we investigated the effect of co-housing Arbor Acres (AA) chickens, a commercial breed susceptible to S. Enteritidis, with Tibetan chickens, a local breed resistant to S. Enteritidis infection, on the resistance of the latter to the pathogen. Ninety-six 1-day-old Tibetan chickens and 96 1-day-old AA chickens were divided into a Tibetan chicken housed alone group (n = 48), an AA chicken housed alone group (n = 48), and a co-housed group (48 birds from each breed for 2 cages). All birds were provided the same diet, and the experimental period lasted 14 d. At d 7, all chickens were infected with S. Enteritidis, and samples were collected at 1-, 3-, and 7-day-post-infection. We found that the body weight of AA chickens significantly increased when co-housed with Tibetan chickens at 1- and 3-day-post-infection (P < 0.05). In addition, the cecal S. Enteritidis load in AA chickens was significantly reduced at 1-, 3-, and 7-day-post-infection (P < 0.05). Furthermore, the inflammatory response in AA chickens decreased, as evidenced by the decreased expression of pro-inflammatory cytokines NOS2, TNF-α, IL-8, IL-1β, and IFN-γ in their cecal tonsils (P < 0.05). Co-housing with Tibetan chickens significantly increased the height of villi and number of goblet cells (P < 0.05), as well as the expression of claudin-1 (P < 0.05), a tight junction protein, in the jejunum of AA chickens. Further analysis revealed that co-housing altered the gut microbiota composition in AA chickens; specifically, the relative abundances of harmful microbes, such as Intestinimonas, Oscillibacter, Tuzzerella, Anaerotruncus, Paludicola, and Anaerofilum were reduced (P < 0.05). Our findings indicate that co-housing with Tibetan chickens enhanced the resistance of AA chickens to S. Enteritidis infection without compromising the resistance of Tibetan chickens. This study provides a novel approach for Salmonella control in practical poultry production.
{"title":"Co-housing with Tibetan chickens improved the resistance of Arbor Acres chickens to Salmonella enterica serovar Enteritidis infection by altering their gut microbiota composition","authors":"Qianyun Zhang, Qidong Zhu, Yunqi Xiao, Qinghua Yu, Shourong Shi","doi":"10.1186/s40104-024-01132-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01132-2","url":null,"abstract":"Salmonella enterica serovar Enteritidis (S. Enteritidis) is a global foodborne pathogen that poses a significant threat to human health, with poultry being the primary reservoir host. Therefore, addressing S. Enteritidis infections in poultry is crucial to protect human health and the poultry industry. In this study, we investigated the effect of co-housing Arbor Acres (AA) chickens, a commercial breed susceptible to S. Enteritidis, with Tibetan chickens, a local breed resistant to S. Enteritidis infection, on the resistance of the latter to the pathogen. Ninety-six 1-day-old Tibetan chickens and 96 1-day-old AA chickens were divided into a Tibetan chicken housed alone group (n = 48), an AA chicken housed alone group (n = 48), and a co-housed group (48 birds from each breed for 2 cages). All birds were provided the same diet, and the experimental period lasted 14 d. At d 7, all chickens were infected with S. Enteritidis, and samples were collected at 1-, 3-, and 7-day-post-infection. We found that the body weight of AA chickens significantly increased when co-housed with Tibetan chickens at 1- and 3-day-post-infection (P < 0.05). In addition, the cecal S. Enteritidis load in AA chickens was significantly reduced at 1-, 3-, and 7-day-post-infection (P < 0.05). Furthermore, the inflammatory response in AA chickens decreased, as evidenced by the decreased expression of pro-inflammatory cytokines NOS2, TNF-α, IL-8, IL-1β, and IFN-γ in their cecal tonsils (P < 0.05). Co-housing with Tibetan chickens significantly increased the height of villi and number of goblet cells (P < 0.05), as well as the expression of claudin-1 (P < 0.05), a tight junction protein, in the jejunum of AA chickens. Further analysis revealed that co-housing altered the gut microbiota composition in AA chickens; specifically, the relative abundances of harmful microbes, such as Intestinimonas, Oscillibacter, Tuzzerella, Anaerotruncus, Paludicola, and Anaerofilum were reduced (P < 0.05). Our findings indicate that co-housing with Tibetan chickens enhanced the resistance of AA chickens to S. Enteritidis infection without compromising the resistance of Tibetan chickens. This study provides a novel approach for Salmonella control in practical poultry production.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142917315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-02DOI: 10.1186/s40104-024-01129-x
Feng Yong, Bo Liu, Huijuan Li, Houxu Hao, Yueli Fan, Osmond Datsomor, Rui Han, Hailong Jiang, Dongsheng Che
There is a growing focus on using various plant-derived agricultural by-products to increase the benefits of pig farming, but these feedstuffs are fibrous in nature. This study investigated the relationship between dietary fiber physicochemical properties and feedstuff fermentation characteristics and their effects on nutrient utilization, energy metabolism, and gut microbiota in growing pigs. Thirty-six growing barrows (47.2 ± 1.5 kg) were randomly allotted to 6 dietary treatments with 2 apparent viscosity levels and 3 β-glucan-to-arabinoxylan ratios. In the experiment, nutrient utilization, energy metabolism, fecal microbial community, and production and absorption of short-chain fatty acid (SCFA) of pigs were investigated. In vitro digestion and fermentation models were used to compare the fermentation characteristics of feedstuffs and ileal digesta in the pig’s hindgut. The production dynamics of SCFA and dry matter corrected gas production of different feedstuffs during in vitro fermentation were different and closely related to the physical properties and chemical structure of the fiber. In animal experiments, increasing the dietary apparent viscosity and the β-glucan-to-arabinoxylan ratios both increased the apparent ileal digestibility (AID), apparent total tract digestibility (ATTD), and hindgut digestibility of fiber components while decreasing the AID and ATTD of dry matter and organic matter (P < 0.05). In addition, increasing dietary apparent viscosity and β-glucan-to-arabinoxylan ratios both increased gas exchange, heat production, and protein oxidation, and decreased energy deposition (P < 0.05). The dietary apparent viscosity and β-glucan-to-arabinoxylan ratios had linear interaction effects on the digestible energy, metabolizable energy, retained energy (RE), and net energy (NE) of the diets (P < 0.05). At the same time, the increase of dietary apparent viscosity and β-glucan-to-arabinoxylan ratios both increased SCFA production and absorption (P < 0.05). Increasing the dietary apparent viscosity and β-glucan-to-arabinoxylan ratios increased the diversity and abundance of bacteria (P < 0.05) and the relative abundance of beneficial bacteria. Furthermore, increasing the dietary β-glucan-to-arabinoxylan ratios led to a linear increase in SCFA production during the in vitro fermentation of ileal digesta (P < 0.001). Finally, the prediction equations for RE and NE were established. Dietary fiber physicochemical properties alter dietary fermentation patterns and regulate nutrient utilization, energy metabolism, and pig gut microbiota composition and metabolites.
{"title":"Relationship between dietary fiber physicochemical properties and feedstuff fermentation characteristics and their effects on nutrient utilization, energy metabolism, and gut microbiota in growing pigs","authors":"Feng Yong, Bo Liu, Huijuan Li, Houxu Hao, Yueli Fan, Osmond Datsomor, Rui Han, Hailong Jiang, Dongsheng Che","doi":"10.1186/s40104-024-01129-x","DOIUrl":"https://doi.org/10.1186/s40104-024-01129-x","url":null,"abstract":"There is a growing focus on using various plant-derived agricultural by-products to increase the benefits of pig farming, but these feedstuffs are fibrous in nature. This study investigated the relationship between dietary fiber physicochemical properties and feedstuff fermentation characteristics and their effects on nutrient utilization, energy metabolism, and gut microbiota in growing pigs. Thirty-six growing barrows (47.2 ± 1.5 kg) were randomly allotted to 6 dietary treatments with 2 apparent viscosity levels and 3 β-glucan-to-arabinoxylan ratios. In the experiment, nutrient utilization, energy metabolism, fecal microbial community, and production and absorption of short-chain fatty acid (SCFA) of pigs were investigated. In vitro digestion and fermentation models were used to compare the fermentation characteristics of feedstuffs and ileal digesta in the pig’s hindgut. The production dynamics of SCFA and dry matter corrected gas production of different feedstuffs during in vitro fermentation were different and closely related to the physical properties and chemical structure of the fiber. In animal experiments, increasing the dietary apparent viscosity and the β-glucan-to-arabinoxylan ratios both increased the apparent ileal digestibility (AID), apparent total tract digestibility (ATTD), and hindgut digestibility of fiber components while decreasing the AID and ATTD of dry matter and organic matter (P < 0.05). In addition, increasing dietary apparent viscosity and β-glucan-to-arabinoxylan ratios both increased gas exchange, heat production, and protein oxidation, and decreased energy deposition (P < 0.05). The dietary apparent viscosity and β-glucan-to-arabinoxylan ratios had linear interaction effects on the digestible energy, metabolizable energy, retained energy (RE), and net energy (NE) of the diets (P < 0.05). At the same time, the increase of dietary apparent viscosity and β-glucan-to-arabinoxylan ratios both increased SCFA production and absorption (P < 0.05). Increasing the dietary apparent viscosity and β-glucan-to-arabinoxylan ratios increased the diversity and abundance of bacteria (P < 0.05) and the relative abundance of beneficial bacteria. Furthermore, increasing the dietary β-glucan-to-arabinoxylan ratios led to a linear increase in SCFA production during the in vitro fermentation of ileal digesta (P < 0.001). Finally, the prediction equations for RE and NE were established. Dietary fiber physicochemical properties alter dietary fermentation patterns and regulate nutrient utilization, energy metabolism, and pig gut microbiota composition and metabolites.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142917178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-20DOI: 10.1186/s40104-024-01128-y
Ludmila Kosaristanova, Zuzana Bytesnikova, Tatiana Fialova, Jana Pekarkova, Pavel Svec, Frantisek Ondreas, Vendula Jemelikova, Andrea Ridoskova, Peter Makovicky, Ladislav Sivak, Monika Dolejska, Monika Zouharova, Petr Slama, Vojtech Adam, Kristyna Smerkova
Bovine mastitis is one of the main causes of reduced production in dairy cows. The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus, whose resistant strains make the treatment of mastitis with conventional antibiotics very difficult and result in high losses. Therefore, it is important to develop novel therapeutic agents to overcome the resistance of mastitis-causing strains. In this study, novel selenium-tellurium based nanoparticles (SeTeNPs) were synthesized and characterized. Their antibacterial activity and biocompatibility were evaluated both in vitro and in vivo using a bovine model. A total of 10 heifers were divided into experimental and control groups (5 animals each). After intramammary infection with methicillin resistant S. aureus (MRSA) and the development of clinical signs of mastitis, a dose of SeTeNPs was administered to all quarters in the experimental group. Based on in vitro tests, the concentration of 149.70 mg/L and 263.95 mg/L of Se and Te, respectively, was used for application into the mammary gland. Three days after SeTeNPs administration, MRSA counts in the experimental group showed a significant reduction (P < 0.01) compared to the control group. The inhibitory effect observed within the in vitro experiments was thus confirmed, resulting in the suppression of infection in animals. Moreover, the superior biocompatibility of SeTeNPs in the organism was demonstrated, as the nanoparticles did not significantly alter the inflammatory response or histopathology at the site of application, i.e., mammary gland, compared to the control group (P > 0.05). Additionally, the metabolic profile of the blood plasma as well as the histology of the main organs remained unaffected, indicating that the nanoparticles had no adverse effects on the organism. Our findings suggest that SeTeNPs can be used as a promising treatment for bovine mastitis in the presence of resistant bacteria. However, the current study is limited by its small sample size, making it primarily a proof of the concept for the efficacy of intramammary-applied SeTeNPs. Therefore, further research with a larger sample size is needed to validate these results.
{"title":"In vivo evaluation of selenium-tellurium based nanoparticles as a novel treatment for bovine mastitis","authors":"Ludmila Kosaristanova, Zuzana Bytesnikova, Tatiana Fialova, Jana Pekarkova, Pavel Svec, Frantisek Ondreas, Vendula Jemelikova, Andrea Ridoskova, Peter Makovicky, Ladislav Sivak, Monika Dolejska, Monika Zouharova, Petr Slama, Vojtech Adam, Kristyna Smerkova","doi":"10.1186/s40104-024-01128-y","DOIUrl":"https://doi.org/10.1186/s40104-024-01128-y","url":null,"abstract":"Bovine mastitis is one of the main causes of reduced production in dairy cows. The infection of the mammary gland is mainly caused by the bacterium Staphylococcus aureus, whose resistant strains make the treatment of mastitis with conventional antibiotics very difficult and result in high losses. Therefore, it is important to develop novel therapeutic agents to overcome the resistance of mastitis-causing strains. In this study, novel selenium-tellurium based nanoparticles (SeTeNPs) were synthesized and characterized. Their antibacterial activity and biocompatibility were evaluated both in vitro and in vivo using a bovine model. A total of 10 heifers were divided into experimental and control groups (5 animals each). After intramammary infection with methicillin resistant S. aureus (MRSA) and the development of clinical signs of mastitis, a dose of SeTeNPs was administered to all quarters in the experimental group. Based on in vitro tests, the concentration of 149.70 mg/L and 263.95 mg/L of Se and Te, respectively, was used for application into the mammary gland. Three days after SeTeNPs administration, MRSA counts in the experimental group showed a significant reduction (P < 0.01) compared to the control group. The inhibitory effect observed within the in vitro experiments was thus confirmed, resulting in the suppression of infection in animals. Moreover, the superior biocompatibility of SeTeNPs in the organism was demonstrated, as the nanoparticles did not significantly alter the inflammatory response or histopathology at the site of application, i.e., mammary gland, compared to the control group (P > 0.05). Additionally, the metabolic profile of the blood plasma as well as the histology of the main organs remained unaffected, indicating that the nanoparticles had no adverse effects on the organism. Our findings suggest that SeTeNPs can be used as a promising treatment for bovine mastitis in the presence of resistant bacteria. However, the current study is limited by its small sample size, making it primarily a proof of the concept for the efficacy of intramammary-applied SeTeNPs. Therefore, further research with a larger sample size is needed to validate these results.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"31 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142858452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Intestinal inflammation is a common and serious health problem in piglet production, especially enteritis caused by pathogenic Escherichia coli (E. coli). This condition often leads to high mortality, slow weight gain, and significant economic losses. In this study, we isolated an E. coli strain, SKLAN202302, from the colon of diarrheal piglets to create an intestinal inflammation model for evaluating the protective effects of baicalin. Piglets infected with E. coli exhibited significant reductions in body weight, feed intake, small intestine length, and ileal goblet cell count (P < 0.05), along with deteriorated ileal morphology. However, baicalin supplementation resulted in body weights, feed intake, and intestinal morphology similar to those of the control group. Notably, there was a significant increase in the colonization of Lactobacillus species, particularly Lactobacillus_reuteri, Lactobacillus_amylovorus, and Lactobacillus_johnii, compared to the E. coli group (P < 0.05). At the metabolic and transcriptional levels, E. coli infection increased inflammatory mediators, including eicosanoids (leukotriene F4, prostaglandin F1a, leukotriene E4, thromboxane B2, prostaglandin G2, and PGH2), monosaccharides, and TCA cycle intermediates (oxoglutaric acid, glutaric acid, adipic acid, citric acid, and isocitric acid) in the ileum. It also promoted the expression of genes related to autoimmune diseases and the Th17 differentiation signaling pathway (CTLA4, IFN-ALPHA-8, IL12RB2, TRAV3, TRAV16, FOS, and VEGFA), as well as inflammatory factors. Conversely, baicalin supplementation not only counteracted these effects but also enhanced the presence of metabolites such as phospholipids [including lysoPC (P-18:1(9Z)/0:0), PC (17:0/0:0), lysoPC (16:1(9Z)/0:0), PC (18:0/0:0), lysoPC (18:0/0:0), PA (10:0/i-16:0), and PA (10:0/8:0)] and amino acids. It also regulated genes within the IL-17 signaling pathway (IL4, CCL17, CXCL10, IFNG, and CXCL2), suggesting a mechanism by which baicalin mitigates E. coli-induced intestinal and microbial disturbances. Subsequent flow cytometry analysis showed that E. coli infection increased the numbers of CD3+ and Foxp3+ cells, decreased IL-17A+ cells, and reduced Th17/Treg ratios. Baicalin supplementation restored these parameters to control levels. Baicalin supplementation effectively alleviates E. coli-induced intestinal inflammation and microbial disturbances in piglets by enhancing beneficial Lactobacillus colonization, counteracting inflammatory mediators, and regulating immune-related gene expression and the Th17/Treg balance. These findings highlight baicalin’s potential in alleviating intestinal inflammation.
{"title":"Baicalin alleviates intestinal inflammation and microbial disturbances by regulating Th17/Treg balance and enhancing Lactobacillus colonization in piglets","authors":"Shunfen Zhang, Chengzeng Luo, Kai Li, Junhong Wang, Huixin Wang, Ruqing Zhong, Liang Chen, Qiugang Ma, Hongfu Zhang","doi":"10.1186/s40104-024-01126-0","DOIUrl":"https://doi.org/10.1186/s40104-024-01126-0","url":null,"abstract":"Intestinal inflammation is a common and serious health problem in piglet production, especially enteritis caused by pathogenic Escherichia coli (E. coli). This condition often leads to high mortality, slow weight gain, and significant economic losses. In this study, we isolated an E. coli strain, SKLAN202302, from the colon of diarrheal piglets to create an intestinal inflammation model for evaluating the protective effects of baicalin. Piglets infected with E. coli exhibited significant reductions in body weight, feed intake, small intestine length, and ileal goblet cell count (P < 0.05), along with deteriorated ileal morphology. However, baicalin supplementation resulted in body weights, feed intake, and intestinal morphology similar to those of the control group. Notably, there was a significant increase in the colonization of Lactobacillus species, particularly Lactobacillus_reuteri, Lactobacillus_amylovorus, and Lactobacillus_johnii, compared to the E. coli group (P < 0.05). At the metabolic and transcriptional levels, E. coli infection increased inflammatory mediators, including eicosanoids (leukotriene F4, prostaglandin F1a, leukotriene E4, thromboxane B2, prostaglandin G2, and PGH2), monosaccharides, and TCA cycle intermediates (oxoglutaric acid, glutaric acid, adipic acid, citric acid, and isocitric acid) in the ileum. It also promoted the expression of genes related to autoimmune diseases and the Th17 differentiation signaling pathway (CTLA4, IFN-ALPHA-8, IL12RB2, TRAV3, TRAV16, FOS, and VEGFA), as well as inflammatory factors. Conversely, baicalin supplementation not only counteracted these effects but also enhanced the presence of metabolites such as phospholipids [including lysoPC (P-18:1(9Z)/0:0), PC (17:0/0:0), lysoPC (16:1(9Z)/0:0), PC (18:0/0:0), lysoPC (18:0/0:0), PA (10:0/i-16:0), and PA (10:0/8:0)] and amino acids. It also regulated genes within the IL-17 signaling pathway (IL4, CCL17, CXCL10, IFNG, and CXCL2), suggesting a mechanism by which baicalin mitigates E. coli-induced intestinal and microbial disturbances. Subsequent flow cytometry analysis showed that E. coli infection increased the numbers of CD3+ and Foxp3+ cells, decreased IL-17A+ cells, and reduced Th17/Treg ratios. Baicalin supplementation restored these parameters to control levels. Baicalin supplementation effectively alleviates E. coli-induced intestinal inflammation and microbial disturbances in piglets by enhancing beneficial Lactobacillus colonization, counteracting inflammatory mediators, and regulating immune-related gene expression and the Th17/Treg balance. These findings highlight baicalin’s potential in alleviating intestinal inflammation. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"91 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142858353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-12-16DOI: 10.1186/s40104-024-01120-6
Wenmin Sheng, Miaomiao Wang, Yuqi Li, Zhenyu Sun, Xing Du, Qifa Li
Oxidative stress (OS) is involved in low female fertility by altering multi-omics such as the transcriptome, miRome, and lncRNome in follicular cells and follicular fluid. However, the mechanism by which OS affects multi-omics dynamics remains largely unknown. Here, we report that OS induces lncRNome dynamics in sow granulosa cells (sGCs), which is partially dependent on the transcription factor activity of its effector, FoxO1. A total of 2,283 putative FoxO recognition elements (FREs) were identified in the promoters of 394 lncRNAs, accounting for 91.20% (394/432) of the lncRNAs regulated by OS. ChIP and reporter assays showed that the effector FoxO1 mediated OS regulation of lncRNA transcription in a transcription factor activity-dependent manner. In sGCs, OS induces the transcription and function (e.g., apoptosis) of NORSF (non-coding RNA involved in sow fertility), a nuclear lncRNA involved in sGC function via FoxO1. Furthermore, FoxO1 has been identified as a transcriptional activator of NORSF in sGCs that interacts with the FRE motif of its promoter. Meanwhile, OS downregulates the transcription of CYP19A1, which encodes an essential enzyme for estrogen synthesis and 17β-estradiol (E2) release by sGCs via the FoxO1 and NORSF axis. Phenotypically, dysregulation of NORSF transcription caused by 2 novel adjacent transitions in the promoter leads to decreased sow fertility. These results suggest a model of OS-stimulated lncRNome dynamics in sGCs and a new signaling pathway of OS that influences sGC function and sow fertility.
{"title":"Oxidative stress controls lncRNA-mediated sow granulosa cell functions in a FoxO1-dependent manner","authors":"Wenmin Sheng, Miaomiao Wang, Yuqi Li, Zhenyu Sun, Xing Du, Qifa Li","doi":"10.1186/s40104-024-01120-6","DOIUrl":"https://doi.org/10.1186/s40104-024-01120-6","url":null,"abstract":"Oxidative stress (OS) is involved in low female fertility by altering multi-omics such as the transcriptome, miRome, and lncRNome in follicular cells and follicular fluid. However, the mechanism by which OS affects multi-omics dynamics remains largely unknown. Here, we report that OS induces lncRNome dynamics in sow granulosa cells (sGCs), which is partially dependent on the transcription factor activity of its effector, FoxO1. A total of 2,283 putative FoxO recognition elements (FREs) were identified in the promoters of 394 lncRNAs, accounting for 91.20% (394/432) of the lncRNAs regulated by OS. ChIP and reporter assays showed that the effector FoxO1 mediated OS regulation of lncRNA transcription in a transcription factor activity-dependent manner. In sGCs, OS induces the transcription and function (e.g., apoptosis) of NORSF (non-coding RNA involved in sow fertility), a nuclear lncRNA involved in sGC function via FoxO1. Furthermore, FoxO1 has been identified as a transcriptional activator of NORSF in sGCs that interacts with the FRE motif of its promoter. Meanwhile, OS downregulates the transcription of CYP19A1, which encodes an essential enzyme for estrogen synthesis and 17β-estradiol (E2) release by sGCs via the FoxO1 and NORSF axis. Phenotypically, dysregulation of NORSF transcription caused by 2 novel adjacent transitions in the promoter leads to decreased sow fertility. These results suggest a model of OS-stimulated lncRNome dynamics in sGCs and a new signaling pathway of OS that influences sGC function and sow fertility.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"40 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142825556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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