Heat stress (HS) incidence is associated with the accumulation of reactive substances, which might be associated with bone loss. N-Acetylcysteine (NAC) exhibits strong antioxidants due to its sulfhydryl group and being as the precursor for endogenous glutathione synthesis. Therefore, interplay between oxidative stress and bone turnover of broilers and the effects of dietary NAC inclusion on antioxidant capability and “gut-bone” axis were evaluated during chronic HS. Implementing cyclic chronic HS (34 °C for 7 h/d) evoked reactive oxygen species excessive production and oxidant stress, which was accompanied by compromised tibia mass. The RNA-seq of proximal tibia also revealed the enrichment of oxidation–reduction process and inflammatory outbursts during HS. Although no notable alterations in the growth performance and cecal microbiota were found, the diet contained 2 g/kg NAC enhanced the antioxidant capability of heat-stressed broiler chickens by upregulating the expression of Nrf2 in the ileum, tibia, and bone marrow. Simultaneously, NAC tended to hinder NF-κB pathway activation and decreased the mRNA levels of the proinflammatory cytokines in both the ileum and bone marrow. As a result, NAC suppressed osteoclastogenesis and osteoclast activity, thereby increasing osteocyte-related gene expression. Furthermore, the inclusion of NAC tended to increase the ash content and density of the whole tibia, as well as improve cortical thickness and bone volume of the diaphysis. These findings HS-mediated outburst of oxidant stress accelerates bone resorption and negatively regulates the bone quality of tibia, which is inhibited by NAC in broilers.
{"title":"Improvement of antioxidant capability by dietary N-acetyl cysteine supplementation alleviates bone loss induced by chronic heat stress in finisher broilers","authors":"Huaiyong Zhang, Herinda Pertiwi, Joris Michiels, Djoere Gaublomme, Maryam Majdeddin, Yuhuang Hou, Matthieu Boone, Dirk Elewaut, Iván Josipovic, Jeroen Degroote","doi":"10.1186/s40104-024-01114-4","DOIUrl":"https://doi.org/10.1186/s40104-024-01114-4","url":null,"abstract":"Heat stress (HS) incidence is associated with the accumulation of reactive substances, which might be associated with bone loss. N-Acetylcysteine (NAC) exhibits strong antioxidants due to its sulfhydryl group and being as the precursor for endogenous glutathione synthesis. Therefore, interplay between oxidative stress and bone turnover of broilers and the effects of dietary NAC inclusion on antioxidant capability and “gut-bone” axis were evaluated during chronic HS. Implementing cyclic chronic HS (34 °C for 7 h/d) evoked reactive oxygen species excessive production and oxidant stress, which was accompanied by compromised tibia mass. The RNA-seq of proximal tibia also revealed the enrichment of oxidation–reduction process and inflammatory outbursts during HS. Although no notable alterations in the growth performance and cecal microbiota were found, the diet contained 2 g/kg NAC enhanced the antioxidant capability of heat-stressed broiler chickens by upregulating the expression of Nrf2 in the ileum, tibia, and bone marrow. Simultaneously, NAC tended to hinder NF-κB pathway activation and decreased the mRNA levels of the proinflammatory cytokines in both the ileum and bone marrow. As a result, NAC suppressed osteoclastogenesis and osteoclast activity, thereby increasing osteocyte-related gene expression. Furthermore, the inclusion of NAC tended to increase the ash content and density of the whole tibia, as well as improve cortical thickness and bone volume of the diaphysis. These findings HS-mediated outburst of oxidant stress accelerates bone resorption and negatively regulates the bone quality of tibia, which is inhibited by NAC in broilers. ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"203 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142756380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-29DOI: 10.1186/s40104-024-01127-z
Seonggyu Bang, Ahmad Yar Qamar, Sung Ho Yun, Na‑Yeon Gu, Heyyoung Kim, Ayeong Han, Heejae Kang, Hye Sun Park, Seung II Kim, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho
<p><b>Correction</b><b>: </b><b>J Animal Sci Biotechnol 15, 145 (2024)</b></p><p><b>https://doi.org/10.1186/s40104-024-01106-4</b></p><br/><p>Following publication of the original article [1], the authors reported an error in authors’ affiliations due to typesetting error, where the 3<sup>rd</sup> and 4<sup>th</sup> institutions are the same. Also, a full stop was erroneously added to author Seung II Kim’s name.</p><p>The full list of authors and affiliations is changed from:</p><p>Seonggyu Bang<sup>1,2</sup>, Ahmad Yar Qamar<sup>3</sup>, Sung Ho Yun<sup>5</sup>, Na-Yeon Gu<sup>6</sup>, Heyyoung Kim<sup>2,7</sup>, Ayeong Han<sup>1,2</sup>, Heejae Kang<sup>1,2</sup>, Hye Sun Park<sup>4</sup>, Seung II. Kim<sup>4</sup>, Islam M. Saadeldin<sup>2,8</sup>, Sanghoon Lee<sup>2</sup> and Jongki Cho<sup>1</sup><sup>*</sup></p><p>1 College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.</p><p>2 College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.</p><p>3 College of Veterinary and Animal Sciences, Jhang Sub-campus of University of Veterinary and Animal Sciences, Lahore, Pakistan.</p><p>4 College of Veterinary and Animal Sciences, Jhang, Sub-Campus of University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.</p><p>5 Korea Basic Science Institute (KBSI), Ochang, Chungcheongbuk‑Do 28119, Republic of Korea.</p><p>6 Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbuk‑Do 39660, Republic of Korea.</p><p>7 Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA)</p><p>Laboratory, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.</p><p>8 Comparative Medicine Department, King Faisal Specialist Hospital & Research Centre, Riyadh 11211, Saudi Arabia.</p><p>To:</p><p>Seonggyu Bang<sup>1,2</sup>, Ahmad Yar Qamar<sup>3</sup>, Sung Ho Yun<sup>4</sup>, Na‑Yeon Gu<sup>5</sup>, Heyyoung Kim<sup>2,6</sup>, Ayeong Han<sup>1,2</sup>, Heejae Kang<sup>1,2</sup>, Hye Sun Park<sup>4</sup>, Seung II Kim<sup>4</sup>, Islam M. Saadeldin<sup>2,7</sup>, Sanghoon Lee<sup>2</sup> and Jongki Cho<sup>1</sup><sup>*</sup></p><p>1 College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.</p><p>2 College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.</p><p>3 College of Veterinary and Animal Sciences, Jhang Sub-campus of University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.</p><p>4 Korea Basic Science Institute (KBSI), Ochang, Chungcheongbuk‑Do 28119, Republic of Korea.</p><p>5 Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbuk‑Do 39660, Republic of Korea.</p><p>6 Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA)</p><p>Laboratory, Johns Hopkins
{"title":"Correction: Embryotrophic effect of exogenous protein contained adipose-derived stem cell extracellular vesicles","authors":"Seonggyu Bang, Ahmad Yar Qamar, Sung Ho Yun, Na‑Yeon Gu, Heyyoung Kim, Ayeong Han, Heejae Kang, Hye Sun Park, Seung II Kim, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho","doi":"10.1186/s40104-024-01127-z","DOIUrl":"https://doi.org/10.1186/s40104-024-01127-z","url":null,"abstract":"<p><b>Correction</b><b>: </b><b>J Animal Sci Biotechnol 15, 145 (2024)</b></p><p><b>https://doi.org/10.1186/s40104-024-01106-4</b></p><br/><p>Following publication of the original article [1], the authors reported an error in authors’ affiliations due to typesetting error, where the 3<sup>rd</sup> and 4<sup>th</sup> institutions are the same. Also, a full stop was erroneously added to author Seung II Kim’s name.</p><p>The full list of authors and affiliations is changed from:</p><p>Seonggyu Bang<sup>1,2</sup>, Ahmad Yar Qamar<sup>3</sup>, Sung Ho Yun<sup>5</sup>, Na-Yeon Gu<sup>6</sup>, Heyyoung Kim<sup>2,7</sup>, Ayeong Han<sup>1,2</sup>, Heejae Kang<sup>1,2</sup>, Hye Sun Park<sup>4</sup>, Seung II. Kim<sup>4</sup>, Islam M. Saadeldin<sup>2,8</sup>, Sanghoon Lee<sup>2</sup> and Jongki Cho<sup>1</sup><sup>*</sup></p><p>1 College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.</p><p>2 College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.</p><p>3 College of Veterinary and Animal Sciences, Jhang Sub-campus of University of Veterinary and Animal Sciences, Lahore, Pakistan.</p><p>4 College of Veterinary and Animal Sciences, Jhang, Sub-Campus of University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.</p><p>5 Korea Basic Science Institute (KBSI), Ochang, Chungcheongbuk‑Do 28119, Republic of Korea.</p><p>6 Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbuk‑Do 39660, Republic of Korea.</p><p>7 Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA)</p><p>Laboratory, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.</p><p>8 Comparative Medicine Department, King Faisal Specialist Hospital & Research Centre, Riyadh 11211, Saudi Arabia.</p><p>To:</p><p>Seonggyu Bang<sup>1,2</sup>, Ahmad Yar Qamar<sup>3</sup>, Sung Ho Yun<sup>4</sup>, Na‑Yeon Gu<sup>5</sup>, Heyyoung Kim<sup>2,6</sup>, Ayeong Han<sup>1,2</sup>, Heejae Kang<sup>1,2</sup>, Hye Sun Park<sup>4</sup>, Seung II Kim<sup>4</sup>, Islam M. Saadeldin<sup>2,7</sup>, Sanghoon Lee<sup>2</sup> and Jongki Cho<sup>1</sup><sup>*</sup></p><p>1 College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea.</p><p>2 College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.</p><p>3 College of Veterinary and Animal Sciences, Jhang Sub-campus of University of Veterinary and Animal Sciences, Lahore 54000, Pakistan.</p><p>4 Korea Basic Science Institute (KBSI), Ochang, Chungcheongbuk‑Do 28119, Republic of Korea.</p><p>5 Viral Disease Research Division, Animal and Plant Quarantine Agency, Gimcheon, Gyeongsangbuk‑Do 39660, Republic of Korea.</p><p>6 Department of Plastic and Reconstructive Surgery, Vascularized Composite Allotransplantation (VCA)</p><p>Laboratory, Johns Hopkins ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"2675 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142753705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<p><b>Correction</b><b>: </b><b>J Animal Sci Biotechnol 15, 133 (2024)</b></p><p><b>https://doi.org/10.1186/s40104-024-01090-9</b></p><br/><p>Following publication of the original article [1], the authors reported errors in the legend of Fig. 7 and the <i>P</i> value of Fig. 8G (0.66 should be corrected to 0.066). </p><p>The originally published legend of Fig. 7 was:</p><p>Effect of PTE on milk composition, antioxidant capacity, inflammatory factors and immunoglobulins. <b>A</b> Colostrum composition. <b>B</b> Colostrum antioxidant capacity. <b>C</b> Colostrum inflammatory factor levels. <b>D</b> Colostrum immunoglobulin levels. <b>E</b> Milk composition. <b>F</b> Milk antioxidant capacity. <b>G</b> Milk inflammatory factor levels. <b>H</b> Milk immunoglobulin levels. CON: control group; PTE: Pterostilbene group. Data are expressed as mean ± SD (<i>n</i> = 6 for each group). <sup>*</sup><i>P</i> < 0.05, compared to the control group.</p><p>The corrected legend of Fig. 7 should read:</p><p>Effect of PTE on milk composition, antioxidant capacity, inflammatory factors and immunoglobulins. <b>A</b> Colostrum composition. <b>B</b> Colostrum antioxidant capacity. <b>C</b> Colostrum inflammatory factor levels and colostrum immunoglobulin levels. <b>D</b> Milk composition. <b>E</b> Milk antioxidant capacity. <b>F</b> Milk inflammatory factor levels and milk immunoglobulin levels. CON: Control group; PTE: Pterostilbene group. Data are expressed as mean ± SD (<i>n</i> = 6 for each group). <sup>*</sup><i>P</i> < 0.05, compared to the control group.</p><p>The originally published Fig. 8 was:</p><figure><figcaption><b data-test="figure-caption-text">Fig. 8</b></figcaption><picture><source srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01124-2/MediaObjects/40104_2024_1124_Fig1_HTML.png?as=webp" type="image/webp"/><img alt="figure 1" aria-describedby="Fig1" height="431" loading="lazy" src="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01124-2/MediaObjects/40104_2024_1124_Fig1_HTML.png" width="685"/></picture><p>Effect of PTE on fecal SCFAs in sows (<b>A</b>–<b>G</b>). CON: control group; PTE: Pterostilbene group. Data are expressed as mean ± SD (<i>n</i> = 6 for each group). <sup>*</sup><i>P</i> < 0.05, compared to the control group</p><span>Full size image</span><svg aria-hidden="true" focusable="false" height="16" role="img" width="16"><use xlink:href="#icon-eds-i-chevron-right-small" xmlns:xlink="http://www.w3.org/1999/xlink"></use></svg></figure><p>The corrected Fig. 8 should read:</p><figure><figcaption><b data-test="figure-caption-text">Fig. 8</b></figcaption><picture><source srcset="//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01124-2/MediaObjects/40104_2024_1124_Fig2_HTML.png?as=webp" type="image/webp"/><img alt="figure 2" aria-describedby="Fig2" height="483" loading="lazy" src="//media.springernature.com/lw685/springer-static/ima
更正：J Animal Sci Biotechnol 15, 133 (2024)https://doi.org/10.1186/s40104-024-01090-9Following 原文[1]发表后，作者报告了图 7 的图例和图 8G 的 P 值错误（0.66 应更正为 0.066）。最初发表的图 7 图例为：PTE 对牛奶成分、抗氧化能力、炎症因子和免疫球蛋白的影响。A 牛初乳成分。B 初乳抗氧化能力。C 牛初乳炎症因子水平。D 初乳免疫球蛋白水平。E 牛奶成分。F 牛奶抗氧化能力G 牛奶炎症因子水平H 牛奶免疫球蛋白水平。CON：对照组；PTE：紫檀芪组。数据以平均值 ± SD 表示（每组 n = 6）。*图 7 更正后的图例应为：PTE 对牛奶成分、抗氧化能力、炎症因子和免疫球蛋白的影响。A 牛初乳成分。B 初乳抗氧化能力。C 初乳炎症因子水平和初乳免疫球蛋白水平。D 牛奶成分。E 牛奶抗氧化能力F 牛奶炎症因子水平和牛奶免疫球蛋白水平。CON：对照组；PTE：紫檀芪组。数据以平均值 ± SD 表示（每组 n = 6）。*图 8 PTE 对母猪粪便 SCFAs 的影响（A-G）。CON：对照组；PTE：紫檀芪组。数据以平均值 ± SD 表示（每组 n = 6）。与对照组相比，*P < 0.05全图经更正后的图 8 应为：图 8 PTE 对母猪粪便 SCFAs 的影响（A-G）。CON：对照组；PTE：紫檀芪组。数据以平均值 ± SD 表示（每组 n = 6）。*Cao M, Bai L, Wei H, et al. Dietary supplementation with pterostilbene activates the PI3K-AKT-mTOR signalling pathway to alleviate progressive oxidative stress and promote placental nutrient transport.J Animal Sci Biotechnol.2024;15:133. https://doi.org/10.1186/s40104-024-01090-9.Article Google Scholar Download references作者及单位东北农业大学动物科技学院，哈尔滨，150030曹明明，白丽云，魏浩云，郭艳彤，孙国栋，孙浩洋&；Baoming Shi作者：Mingming Cao查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Liyun Bai查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Haoyun Wei查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者Yantong Guo查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者在 PubMed Google Scholar中查看作者发表的文章您也可以在 PubMed Google Scholar中搜索该作者孙浩洋在 PubMed Google Scholar中查看作者发表的文章您也可以在 PubMed Google Scholar中搜索该作者石宝明在 PubMed Google Scholar中查看作者发表的文章您也可以在 PubMed Google Scholar中搜索该作者通讯作者给孙浩洋或石宝明的回信。开放存取 本文采用知识共享署名 4.0 国际许可协议进行许可，该协议允许以任何媒介或格式使用、共享、改编、分发和复制本文，但必须注明原作者和出处，提供知识共享许可协议的链接，并说明是否进行了修改。本文中的图片或其他第三方材料均包含在文章的知识共享许可协议中，除非在材料的署名栏中另有说明。如果材料未包含在文章的知识共享许可协议中，且您打算使用的材料不符合法律规定或超出许可使用范围，则您需要直接从版权所有者处获得许可。要查看该许可的副本，请访问 http://creativecommons.org/licenses/by/4.0/。除非在数据的信用行中另有说明，否则知识共享公共领域专用免责声明 (http://creativecommons.org/publicdomain/zero/1.0/) 适用于本文提供的数据。转载与许可引用本文Cao, M., Bai, L., Wei, H. et al. Correction：膳食补充紫檀芪可激活PI3K-AKT-mTOR信号通路，缓解进行性氧化应激，促进胎盘营养运输。J Animal Sci Biotechnol 15, 161 (2024). https://doi.org/10.1186/s40104-024-01124-2Download citationPublished: 25 November 2024DOI: https://doi.org/10.1186/s40104-024-01124-2Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative.
{"title":"Correction: Dietary supplementation with pterostilbene activates the PI3K-AKT-mTOR signalling pathway to alleviate progressive oxidative stress and promote placental nutrient transport","authors":"Mingming Cao, Liyun Bai, Haoyun Wei, Yantong Guo, Guodong Sun, Haoyang Sun, Baoming Shi","doi":"10.1186/s40104-024-01124-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01124-2","url":null,"abstract":"<p><b>Correction</b><b>: </b><b>J Animal Sci Biotechnol 15, 133 (2024)</b></p><p><b>https://doi.org/10.1186/s40104-024-01090-9</b></p><br/><p>Following publication of the original article [1], the authors reported errors in the legend of Fig. 7 and the <i>P</i> value of Fig. 8G (0.66 should be corrected to 0.066).\u0000</p><p>The originally published legend of Fig. 7 was:</p><p>Effect of PTE on milk composition, antioxidant capacity, inflammatory factors and immunoglobulins. <b>A</b> Colostrum composition. <b>B</b> Colostrum antioxidant capacity. <b>C</b> Colostrum inflammatory factor levels. <b>D</b> Colostrum immunoglobulin levels. <b>E</b> Milk composition. <b>F</b> Milk antioxidant capacity. <b>G</b> Milk inflammatory factor levels. <b>H</b> Milk immunoglobulin levels. CON: control group; PTE: Pterostilbene group. Data are expressed as mean ± SD (<i>n</i> = 6 for each group). <sup>*</sup><i>P</i> < 0.05, compared to the control group.</p><p>The corrected legend of Fig. 7 should read:</p><p>Effect of PTE on milk composition, antioxidant capacity, inflammatory factors and immunoglobulins. <b>A</b> Colostrum composition. <b>B</b> Colostrum antioxidant capacity. <b>C</b> Colostrum inflammatory factor levels and colostrum immunoglobulin levels. <b>D</b> Milk composition. <b>E</b> Milk antioxidant capacity. <b>F</b> Milk inflammatory factor levels and milk immunoglobulin levels. CON: Control group; PTE: Pterostilbene group. Data are expressed as mean ± SD (<i>n</i> = 6 for each group). <sup>*</sup><i>P</i> < 0.05, compared to the control group.</p><p>The originally published Fig. 8 was:</p><figure><figcaption><b data-test=\"figure-caption-text\">Fig. 8</b></figcaption><picture><source srcset=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01124-2/MediaObjects/40104_2024_1124_Fig1_HTML.png?as=webp\" type=\"image/webp\"/><img alt=\"figure 1\" aria-describedby=\"Fig1\" height=\"431\" loading=\"lazy\" src=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01124-2/MediaObjects/40104_2024_1124_Fig1_HTML.png\" width=\"685\"/></picture><p>Effect of PTE on fecal SCFAs in sows (<b>A</b>–<b>G</b>). CON: control group; PTE: Pterostilbene group. Data are expressed as mean ± SD (<i>n</i> = 6 for each group). <sup>*</sup><i>P</i> < 0.05, compared to the control group</p><span>Full size image</span><svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-chevron-right-small\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></figure><p>The corrected Fig. 8 should read:</p><figure><figcaption><b data-test=\"figure-caption-text\">Fig. 8</b></figcaption><picture><source srcset=\"//media.springernature.com/lw685/springer-static/image/art%3A10.1186%2Fs40104-024-01124-2/MediaObjects/40104_2024_1124_Fig2_HTML.png?as=webp\" type=\"image/webp\"/><img alt=\"figure 2\" aria-describedby=\"Fig2\" height=\"483\" loading=\"lazy\" src=\"//media.springernature.com/lw685/springer-static/ima","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"256 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-14DOI: 10.1186/s40104-024-01108-2
Bin Wang, Xiaodan Zhang, Yongfa Liu, Mingkun Gao, Mi Wang, Yuan Wang, Xinzhi Wang, Yuming Guo
Research on low-protein-level diets has indicated that even though the profiles of essential amino acids (EAAs) follow the recommendation for a normal-protein-level diet, broilers fed low-protein diets failed to achieve productive performance compared to those fed normal diets. Therefore, it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance. Furthermore, identifying novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acid nutrition and application technology. In this study, 12 dietary treatments [Con(+), Con(-), L&A(-), L&A(+), M&C(-), M&C(+), BCAA (-), BCAA(+), Thr(-), Thr(+), Trp(-) and Trp(+)] were established by combining different EAAs including lysine and arginine, methionine and cysteine, branched-chain amino acid (BCAA), threonine, and tryptophan to observe the growth and development of the broiler chickens fed with low-protein-level diets. Based on the biochemical parameters and untargeted metabolomic analysis of animals subjected to different treatments, biomarkers associated with optimal and suboptimal amino acid balance were identified. Growth performance, carcass characteristics, hepatic enzyme activity, serum biochemical parameters, and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns. Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns than female broilers. For the low-protein diet, the dietary concentrations of lysine, arginine, and tryptophan, but not of methionine, cystine, or threonine, needed to be increased. Therefore, further research on individual BCAA is required. For untargeted metabolomic analysis, Con(+) was selected as a normal diet (NP) while Con(-) represented a low-protein diet (LP). L&A(+) denotes a low-protein amino acid balanced diet (LPAB) and Thr(+) represents a low-protein amino acid imbalance diet (LPAI). The metabolites oxypurinol, pantothenic acid, and D-octopine in birds were significantly influenced by different dietary amino acid patterns. Adjusting the amino acid profile of low-protein diets is required to achieve normal growth performance in broiler chickens fed normal-protein diets. Oxypurinol, pantothenic acid, and D-octopine have been identified as potentially sensitive biomarkers for assessing amino acid balance.
{"title":"Assessment of the dietary amino acid profiles and the relative biomarkers for amino acid balance in the low-protein diets for broiler chickens","authors":"Bin Wang, Xiaodan Zhang, Yongfa Liu, Mingkun Gao, Mi Wang, Yuan Wang, Xinzhi Wang, Yuming Guo","doi":"10.1186/s40104-024-01108-2","DOIUrl":"https://doi.org/10.1186/s40104-024-01108-2","url":null,"abstract":"Research on low-protein-level diets has indicated that even though the profiles of essential amino acids (EAAs) follow the recommendation for a normal-protein-level diet, broilers fed low-protein diets failed to achieve productive performance compared to those fed normal diets. Therefore, it is imperative to reassess the optimum profile of EAAs in low-protein diets and establish a new ideal pattern for amino acid balance. Furthermore, identifying novel sensitive biomarkers for assessing amino acid balance will greatly facilitate the development of amino acid nutrition and application technology. In this study, 12 dietary treatments [Con(+), Con(-), L&A(-), L&A(+), M&C(-), M&C(+), BCAA (-), BCAA(+), Thr(-), Thr(+), Trp(-) and Trp(+)] were established by combining different EAAs including lysine and arginine, methionine and cysteine, branched-chain amino acid (BCAA), threonine, and tryptophan to observe the growth and development of the broiler chickens fed with low-protein-level diets. Based on the biochemical parameters and untargeted metabolomic analysis of animals subjected to different treatments, biomarkers associated with optimal and suboptimal amino acid balance were identified. Growth performance, carcass characteristics, hepatic enzyme activity, serum biochemical parameters, and breast muscle mRNA expression differed significantly between male and female broilers under different dietary amino acid patterns. Male broilers exhibited higher sensitivity to the adjustment of amino acid patterns than female broilers. For the low-protein diet, the dietary concentrations of lysine, arginine, and tryptophan, but not of methionine, cystine, or threonine, needed to be increased. Therefore, further research on individual BCAA is required. For untargeted metabolomic analysis, Con(+) was selected as a normal diet (NP) while Con(-) represented a low-protein diet (LP). L&A(+) denotes a low-protein amino acid balanced diet (LPAB) and Thr(+) represents a low-protein amino acid imbalance diet (LPAI). The metabolites oxypurinol, pantothenic acid, and D-octopine in birds were significantly influenced by different dietary amino acid patterns. Adjusting the amino acid profile of low-protein diets is required to achieve normal growth performance in broiler chickens fed normal-protein diets. Oxypurinol, pantothenic acid, and D-octopine have been identified as potentially sensitive biomarkers for assessing amino acid balance.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"35 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The antibacterial and immunomodulatory activities of bacteriocins make them attractive targets for development as anti-infective drugs. Although the importance of the enteric nervous system (ENS) in the struggle against infections of the intestine has been demonstrated, whether it is involved in bacteriocins anti-infective mechanisms is poorly defined. Here, we demonstrated that the bacteriocin Microcin J25 (J25) significantly alleviated diarrhea and intestinal inflammation in piglets caused by enterotoxigenic Escherichia coli (ETEC) infection. Mechanistically, macrophage levels were significantly downregulated after J25 treatment, and this was replicated in a mouse model. Omics analysis and validation screening revealed that J25 treatment induced significant changes in the dopaminergic neuron pathway, but little change in microbial structure. The alleviation of inflammation may occur by down-regulating dopamine receptor (DR) D1 and the downstream DAG-PKC pathway, thus inhibiting arachidonic acid decomposition, and the inhibition of macrophages may occur through the up-regulation of DRD5 and the downstream cAMP-PKA pathway, thus inhibiting NF-κB. Our studies’ findings provide insight into the changes and possible roles of the ENS in J25 treatment of ETEC infection, providing a more sophisticated foundational understanding for developing the application potential of J25.
{"title":"Bacteriocin Microcin J25’s antibacterial infection effects and novel non-microbial regulatory mechanisms: differential regulation of dopaminergic receptors","authors":"Lijun Shang, Fengjuan Yang, Qingyun Chen, Ziqi Dai, Guangxin Yang, Xiangfang Zeng, Shiyan Qiao, Haitao Yu","doi":"10.1186/s40104-024-01115-3","DOIUrl":"https://doi.org/10.1186/s40104-024-01115-3","url":null,"abstract":"The antibacterial and immunomodulatory activities of bacteriocins make them attractive targets for development as anti-infective drugs. Although the importance of the enteric nervous system (ENS) in the struggle against infections of the intestine has been demonstrated, whether it is involved in bacteriocins anti-infective mechanisms is poorly defined. Here, we demonstrated that the bacteriocin Microcin J25 (J25) significantly alleviated diarrhea and intestinal inflammation in piglets caused by enterotoxigenic Escherichia coli (ETEC) infection. Mechanistically, macrophage levels were significantly downregulated after J25 treatment, and this was replicated in a mouse model. Omics analysis and validation screening revealed that J25 treatment induced significant changes in the dopaminergic neuron pathway, but little change in microbial structure. The alleviation of inflammation may occur by down-regulating dopamine receptor (DR) D1 and the downstream DAG-PKC pathway, thus inhibiting arachidonic acid decomposition, and the inhibition of macrophages may occur through the up-regulation of DRD5 and the downstream cAMP-PKA pathway, thus inhibiting NF-κB. Our studies’ findings provide insight into the changes and possible roles of the ENS in J25 treatment of ETEC infection, providing a more sophisticated foundational understanding for developing the application potential of J25.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"69 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-12DOI: 10.1186/s40104-024-01113-5
Dan Hu, Xiaoran Yang, Ming Qin, Li’an Pan, Haiyan Fang, Pengnan Chen, Yingdong Ni
Salmonella Typhimurium (S. Typhimurium) is a common pathogenic microorganism and poses a threat to the efficiency of poultry farms. As signaling molecules regulating the interaction between the host and gut microbiota, bile acids (BAs) play a protective role in maintaining gut homeostasis. However, the antibacterial effect of BAs on Salmonella infection in broilers has remained unexplored. Therefore, the aim of this study was to investigate the potential role of feeding BAs in protecting against S. Typhimurium infection in broilers. A total of 144 1-day-old Arbor Acres male broilers were randomly assigned to 4 groups, including non-challenged birds fed a basal diet (CON), S. Typhimurium-challenged birds (ST), S. Typhimurium-challenged birds treated with 0.15 g/kg antibiotic after infection (ST-ANT), and S. Typhimurium-challenged birds fed a basal diet supplemented with 350 mg/kg of BAs (ST-BA). BAs supplementation ameliorated weight loss induced by S. Typhimurium infection and reduced the colonization of Salmonella in the liver and small intestine in broilers (P < 0.05). Compared to the ST group, broilers in ST-BA group had a higher ileal mucosal thickness and villus height, and BAs also ameliorated the increase of diamine oxidase (DAO) level in serum (P < 0.05). It was observed that the mucus layer thickness and the number of villous and cryptic goblet cells (GCs) were increased in the ST-BA group, consistent with the upregulation of MUC2 gene expression in the ileal mucosa (P < 0.05). Moreover, the mRNA expressions of Toll-like receptor 5 (TLR5), Toll-like receptor 4 (TLR4), and interleukin 1 beta (IL1b) were downregulated in the ileum by BAs treatment (P < 0.05). 16S rDNA sequencing analysis revealed that, compared to ST group, BAs ameliorated the decreases in Bacteroidota, Bacteroidaceae and Bacteroides abundances, which were negatively correlated with serum DAO activity, and the increases in Campylobacterota, Campylobacteraceae and Campylobacter abundances, which were negatively correlated with body weight but positively correlated with serum D-lactic acid (D-LA) levels (P < 0.05). Dietary BAs supplementation strengthens the intestinal mucosal barrier and reverses dysbiosis of gut microbiota, which eventually relieves the damage to the intestinal barrier and weight loss induced by S. Typhimurium infection in broilers.
鼠伤寒沙门氏菌（S. Typhimurium）是一种常见的病原微生物，对家禽养殖场的效率构成威胁。作为调节宿主与肠道微生物群之间相互作用的信号分子，胆汁酸（BAs）在维持肠道平衡方面发挥着保护作用。然而，胆汁酸对肉鸡沙门氏菌感染的抗菌作用仍有待探索。因此，本研究旨在探讨饲喂 BAs 在防止肉鸡感染鼠伤寒沙门氏菌方面的潜在作用。研究人员将144只1日龄的Arbor Acres雄性肉鸡随机分为4组，包括饲喂基础日粮（CON）的未受感染鸡、受鼠伤寒杆菌感染的鸡（ST）、受鼠伤寒杆菌感染后使用0.15克/千克抗生素治疗的鸡（ST-ANT）以及饲喂添加了350毫克/千克BAs的基础日粮（ST-BA）的受鼠伤寒杆菌感染的鸡（ST-BA）。补充 BAs 可改善伤寒杆菌感染引起的体重下降，并减少沙门氏菌在肉鸡肝脏和小肠中的定植（P < 0.05）。与 ST 组相比，ST-BA 组肉鸡的回肠粘膜厚度和绒毛高度更高，而且 BA 还能改善血清中二胺氧化酶（DAO）水平的升高（P < 0.05）。观察发现，ST-BA 组黏液层厚度、绒毛和隐性鹅口疮细胞（GCs）数量增加，与回肠黏膜 MUC2 基因表达上调一致（P < 0.05）。此外，Toll 样受体 5（TLR5）、Toll 样受体 4（TLR4）和白细胞介素 1 beta（IL1b）的 mRNA 表达在 BAs 处理后的回肠中下调（P < 0.05）。16S rDNA 测序分析表明，与 ST 组相比，BAs 可改善类杆菌属、类杆菌科和类杆菌丰度的下降（与血清 DAO 活性呈负相关），以及弯曲杆菌属、弯曲杆菌科和弯曲杆菌丰度的增加（与体重呈负相关，但与血清 D-乳酸（D-LA）水平呈正相关）（P < 0.05）。膳食中补充 BAs 可增强肠道黏膜屏障，逆转肠道微生物区系失调，最终缓解伤寒杆菌感染对肉鸡肠道屏障的破坏和体重下降。
{"title":"Dietary bile acids supplementation protects against Salmonella Typhimurium infection via improving intestinal mucosal barrier and gut microbiota composition in broilers","authors":"Dan Hu, Xiaoran Yang, Ming Qin, Li’an Pan, Haiyan Fang, Pengnan Chen, Yingdong Ni","doi":"10.1186/s40104-024-01113-5","DOIUrl":"https://doi.org/10.1186/s40104-024-01113-5","url":null,"abstract":"Salmonella Typhimurium (S. Typhimurium) is a common pathogenic microorganism and poses a threat to the efficiency of poultry farms. As signaling molecules regulating the interaction between the host and gut microbiota, bile acids (BAs) play a protective role in maintaining gut homeostasis. However, the antibacterial effect of BAs on Salmonella infection in broilers has remained unexplored. Therefore, the aim of this study was to investigate the potential role of feeding BAs in protecting against S. Typhimurium infection in broilers. A total of 144 1-day-old Arbor Acres male broilers were randomly assigned to 4 groups, including non-challenged birds fed a basal diet (CON), S. Typhimurium-challenged birds (ST), S. Typhimurium-challenged birds treated with 0.15 g/kg antibiotic after infection (ST-ANT), and S. Typhimurium-challenged birds fed a basal diet supplemented with 350 mg/kg of BAs (ST-BA). BAs supplementation ameliorated weight loss induced by S. Typhimurium infection and reduced the colonization of Salmonella in the liver and small intestine in broilers (P < 0.05). Compared to the ST group, broilers in ST-BA group had a higher ileal mucosal thickness and villus height, and BAs also ameliorated the increase of diamine oxidase (DAO) level in serum (P < 0.05). It was observed that the mucus layer thickness and the number of villous and cryptic goblet cells (GCs) were increased in the ST-BA group, consistent with the upregulation of MUC2 gene expression in the ileal mucosa (P < 0.05). Moreover, the mRNA expressions of Toll-like receptor 5 (TLR5), Toll-like receptor 4 (TLR4), and interleukin 1 beta (IL1b) were downregulated in the ileum by BAs treatment (P < 0.05). 16S rDNA sequencing analysis revealed that, compared to ST group, BAs ameliorated the decreases in Bacteroidota, Bacteroidaceae and Bacteroides abundances, which were negatively correlated with serum DAO activity, and the increases in Campylobacterota, Campylobacteraceae and Campylobacter abundances, which were negatively correlated with body weight but positively correlated with serum D-lactic acid (D-LA) levels (P < 0.05). Dietary BAs supplementation strengthens the intestinal mucosal barrier and reverses dysbiosis of gut microbiota, which eventually relieves the damage to the intestinal barrier and weight loss induced by S. Typhimurium infection in broilers.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"4 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142599665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-11DOI: 10.1186/s40104-024-01123-3
Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Haifang Li, Yunlei Zhou, Aizhi Cao, Jianmin Wang, Xiaojuan Wang, Hai Lin
<p> <b>Correction</b><b>: </b> <b>J Animal Sci Biotechnol 15, 113 (2024)</b> </p><p><b>https://doi.org/10.1186/s40104-024-01071-y</b></p><br/><p>Following publication of the original article [1], the authors reported a typo in the author name.</p><p>The author name <b>Jianming Wang</b> should be corrected to <b>Jianmin Wang</b>.</p><p>The original article [1] has been updated.</p><ol data-track-component="outbound reference" data-track-context="references section"><li data-counter="1."><p>Wang M, Li K, Jiao H, et al. Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens. J Animal Sci Biotechnol. 2024;15:113. https://doi.org/10.1186/s40104-024-01071-y.</p><p>Article CAS Google Scholar </p></li></ol><p>Download references<svg aria-hidden="true" focusable="false" height="16" role="img" width="16"><use xlink:href="#icon-eds-i-download-medium" xmlns:xlink="http://www.w3.org/1999/xlink"></use></svg></p><h3>Authors and Affiliations</h3><ol><li><p>College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Xiaojuan Wang & Hai Lin</p></li><li><p>College of Life Sciences, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Haifang Li</p></li><li><p>College of Chemistry and Material Science, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Yunlei Zhou</p></li><li><p>Shandong Longchang Animal Health Products Co., Ltd., Jinan, P. R. China</p><p>Aizhi Cao & Jianmin Wang</p></li></ol><span>Authors</span><ol><li><span>Minghui Wang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Kelin Li</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Hongchao Jiao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jingpeng Zhao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Haifang Li</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Yunlei Zhou</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Aizhi Cao</span>View author publications<p>You can also search for
更正：J Animal Sci Biotechnol 15, 113 (2024)https://doi.org/10.1186/s40104-024-01071-yFollowing 原文[1]发表时，作者报告了作者姓名中的一个错字。作者姓名Jianming Wang应更正为Jianmin Wang。原文[1]已更新。Wang M, Li K, Jiao H, et al. Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens.J Animal Sci Biotechnol.2024;15:113. https://doi.org/10.1186/s40104-024-01071-y.Article CAS Google Scholar 下载参考文献作者与单位山东农业大学动物科技学院，山东省动物生物技术与疫病防控重点实验室，农业农村部非粮饲料资源高效利用重点实验室（部省共建），山东农业大学，泰安市岱宗大街61号。山东省泰安市岱宗大街 61 号 山东农业大学生命科学学院 王明辉 李克林 焦洪超 赵景鹏 王晓娟 & Hai LinCollege of Life Sciences, Shandong Agricultural University, No.李海芳 山东省泰安市岱宗大街 61 号山东农业大学化学与材料科学学院 邮编：271018 周云雷 山东龙昌动物保健品有限公司，泰安市岱岳区岱宗大街 61 号、山东龙昌动物保健品有限公司中国济南中国曹爱芝 &；王建民作者：王明辉查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者李克林查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者焦洪超查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者赵景鹏查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者李海芳查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者周云雷查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者周云雷查看作者发表的论文您也可以在PubMed Google Scholar中搜索该作者周云雷查看作者发表的论文发表文章您也可以在PubMed Google Scholar中搜索该作者曹爱芝查看发表文章您也可以在PubMed Google Scholar中搜索该作者王建民查看发表文章您也可以在PubMed Google Scholar中搜索该作者王晓娟查看发表文章您也可以在PubMed Google Scholar中搜索该作者林海查看发表文章您也可以在PubMed Google Scholar中搜索该作者通讯作者：王晓娟或林海。开放存取 本文采用知识共享署名 4.0 国际许可协议进行许可，该协议允许以任何媒介或格式使用、共享、改编、分发和复制本文，但需注明原作者和出处，提供知识共享许可协议的链接，并说明是否进行了修改。本文中的图片或其他第三方材料均包含在文章的知识共享许可协议中，除非在材料的署名栏中另有说明。如果材料未包含在文章的知识共享许可协议中，且您打算使用的材料不符合法律规定或超出许可使用范围，则您需要直接从版权所有者处获得许可。要查看该许可的副本，请访问 http://creativecommons.org/licenses/by/4.0/。除非在数据的信用行中另有说明，否则知识共享公共领域专用免责声明（http://creativecommons.org/publicdomain/zero/1.0/）适用于本文提供的数据。转载和许可引用本文Wang, M., Li, K., Jiao, H. et al. Correction：补充胆汁酸可减少肉鸡肝脏脂肪沉积，肠道微生物群和肝脏胆汁酸谱改变参与其中J Animal Sci Biotechnol 15, 154 (2024). https://doi.org/10.1186/s40104-024-01123-3Download citationPublished: 11 November 2024DOI: https://doi.org/10.1186/s40104-024-01123-3Share this articleAnyone you share the following link with will be able to read this content:Get shareable linkSorry, a shareable link is not currently available for this article.Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative
{"title":"Correction: Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens","authors":"Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Haifang Li, Yunlei Zhou, Aizhi Cao, Jianmin Wang, Xiaojuan Wang, Hai Lin","doi":"10.1186/s40104-024-01123-3","DOIUrl":"https://doi.org/10.1186/s40104-024-01123-3","url":null,"abstract":"<p>\u0000<b>Correction</b><b>: </b>\u0000<b>J Animal Sci Biotechnol 15, 113 (2024)</b>\u0000</p><p><b>https://doi.org/10.1186/s40104-024-01071-y</b></p><br/><p>Following publication of the original article [1], the authors reported a typo in the author name.</p><p>The author name <b>Jianming Wang</b> should be corrected to <b>Jianmin Wang</b>.</p><p>The original article [1] has been updated.</p><ol data-track-component=\"outbound reference\" data-track-context=\"references section\"><li data-counter=\"1.\"><p>Wang M, Li K, Jiao H, et al. Dietary bile acids supplementation decreases hepatic fat deposition with the involvement of altered gut microbiota and liver bile acids profile in broiler chickens. J Animal Sci Biotechnol. 2024;15:113. https://doi.org/10.1186/s40104-024-01071-y.</p><p>Article CAS Google Scholar </p></li></ol><p>Download references<svg aria-hidden=\"true\" focusable=\"false\" height=\"16\" role=\"img\" width=\"16\"><use xlink:href=\"#icon-eds-i-download-medium\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"></use></svg></p><h3>Authors and Affiliations</h3><ol><li><p>College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction By Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Minghui Wang, Kelin Li, Hongchao Jiao, Jingpeng Zhao, Xiaojuan Wang & Hai Lin</p></li><li><p>College of Life Sciences, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Haifang Li</p></li><li><p>College of Chemistry and Material Science, Shandong Agricultural University, No. 61, Daizong Street, Taian, 271018, Shandong, P. R. China</p><p>Yunlei Zhou</p></li><li><p>Shandong Longchang Animal Health Products Co., Ltd., Jinan, P. R. China</p><p>Aizhi Cao & Jianmin Wang</p></li></ol><span>Authors</span><ol><li><span>Minghui Wang</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Kelin Li</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Hongchao Jiao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Jingpeng Zhao</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Haifang Li</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Yunlei Zhou</span>View author publications<p>You can also search for this author in <span>PubMed<span> </span>Google Scholar</span></p></li><li><span>Aizhi Cao</span>View author publications<p>You can also search for ","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"69 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142598476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-10DOI: 10.1186/s40104-024-01112-6
Gaiping Wen, Klaus Eder, Robert Ringseis
The use of conventional two-dimensional (2D) culture of the porcine intestinal epithelial cell (IEC) line IPEC-J2 in animal nutrition research has the disadvantage that IEC function is studied under unphysiological conditions, which limits the ability of transferring knowledge to the in vivo-situation. Thus, the aim of the present study was to establish a more convincing and meaningful three-dimensional (3D) culture of IPEC-J2 cells, which allows to study cell function in a more tissue-like environment, and to compare the effect of the endoplasmic reticulum (ER) stress inducer tunicamycin (TM) on ER stress indicators and the expression of tight junction proteins (TJP), inflammatory and apoptosis-related genes and the modulatory role of 1,25-dihydroxy-vitamin D3 (1,25D3) on these parameters in 2D and 3D cultures of IPEC-J2 cells. A published protocol for 3D culture of Caco-2 cells was successfully adopted to IPEC-J2 cells as evident from fully differentiated 3D IPEC-J2 spheroids showing the characteristic spherical architecture with a single layer of IPEC-J2 cells surrounding a central lumen. Treatment of 2D IPEC-J2 cells and 3D IPEC-J2 spheroids with TM for 24 h markedly increased mRNA and/or protein levels of the ER stress target genes, heat shock protein family A (Hsp70) member 5 (HSPA5) and DNA damage inducible transcript 3 (DDIT3), whereas co-treatment with TM and 1,25D3 did not mitigate TM-induced ER stress in IPEC-J2 cells in the 2D and the 3D cell culture. In contrast, TM-induced expression of pro-inflammatory [interleukin-6 (IL6), IL8] and pro-apoptotic genes [BCL2 associated X, apoptosis regulator (BAX), caspase 3 (CASP3), CASP8] and genes encoding TJP [TJP1, claudin 1 (CLDN1), CLDN3, occludin (OCLN), cadherin 1 (CDH1), junctional adhesion molecule 1 (JAM1)] was reduced by co-treatment with TM and 1,25D3 in 3D IPEC-J2 spheroids but not in the 2D cell culture. The effect of 1,25D3 in the IPEC-J2 cell culture is dependent on the culture model applied. While 1,25D3 does not inhibit TM-induced expression of genes involved in inflammation, apoptosis and TJP in conventional 2D cultures of IPEC-J2 cells, TM-induced expression of these genes is abrogated by 1,25D3 in the more meaningful 3D IPEC-J2 cell culture model.
{"title":"Comparative evaluation of the modulatory role of 1,25-dihydroxy-vitamin D3 on endoplasmic reticulum stress-induced effects in 2D and 3D cultures of the intestinal porcine epithelial cell line IPEC-J2","authors":"Gaiping Wen, Klaus Eder, Robert Ringseis","doi":"10.1186/s40104-024-01112-6","DOIUrl":"https://doi.org/10.1186/s40104-024-01112-6","url":null,"abstract":"The use of conventional two-dimensional (2D) culture of the porcine intestinal epithelial cell (IEC) line IPEC-J2 in animal nutrition research has the disadvantage that IEC function is studied under unphysiological conditions, which limits the ability of transferring knowledge to the in vivo-situation. Thus, the aim of the present study was to establish a more convincing and meaningful three-dimensional (3D) culture of IPEC-J2 cells, which allows to study cell function in a more tissue-like environment, and to compare the effect of the endoplasmic reticulum (ER) stress inducer tunicamycin (TM) on ER stress indicators and the expression of tight junction proteins (TJP), inflammatory and apoptosis-related genes and the modulatory role of 1,25-dihydroxy-vitamin D3 (1,25D3) on these parameters in 2D and 3D cultures of IPEC-J2 cells. A published protocol for 3D culture of Caco-2 cells was successfully adopted to IPEC-J2 cells as evident from fully differentiated 3D IPEC-J2 spheroids showing the characteristic spherical architecture with a single layer of IPEC-J2 cells surrounding a central lumen. Treatment of 2D IPEC-J2 cells and 3D IPEC-J2 spheroids with TM for 24 h markedly increased mRNA and/or protein levels of the ER stress target genes, heat shock protein family A (Hsp70) member 5 (HSPA5) and DNA damage inducible transcript 3 (DDIT3), whereas co-treatment with TM and 1,25D3 did not mitigate TM-induced ER stress in IPEC-J2 cells in the 2D and the 3D cell culture. In contrast, TM-induced expression of pro-inflammatory [interleukin-6 (IL6), IL8] and pro-apoptotic genes [BCL2 associated X, apoptosis regulator (BAX), caspase 3 (CASP3), CASP8] and genes encoding TJP [TJP1, claudin 1 (CLDN1), CLDN3, occludin (OCLN), cadherin 1 (CDH1), junctional adhesion molecule 1 (JAM1)] was reduced by co-treatment with TM and 1,25D3 in 3D IPEC-J2 spheroids but not in the 2D cell culture. The effect of 1,25D3 in the IPEC-J2 cell culture is dependent on the culture model applied. While 1,25D3 does not inhibit TM-induced expression of genes involved in inflammation, apoptosis and TJP in conventional 2D cultures of IPEC-J2 cells, TM-induced expression of these genes is abrogated by 1,25D3 in the more meaningful 3D IPEC-J2 cell culture model.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"196 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During the transition period, excessive negative energy balance (NEB) lead to metabolic disorders and reduced milk yield. Rumen microbes are responsible for resolving plant material and producing volatile fatty acids (VFA), which are the primary energy source for cows. In this study, we aimed to investigate the effect of citrus peel extract (CPE) supplementation on rumen microbiota composition, energy metabolism and milk performance of peripartum dairy cows. Dairy cows were fed either a basal diet (CON group) or the same basal diet supplemented with CPE via intragastric administration (4 g/d, CPE group) for 6 weeks (3 weeks before and 3 weeks after calving; n = 15 per group). Samples of serum, milk, rumen fluid, adipose tissue, and liver were collected to assess the effects of CPE on rumen microbiota composition, rumen fermentation parameters, milk performance, and energy metabolic status of dairy cows. CPE supplementation led to an increase in milk yield, milk protein and lactose contents, and serum glucose levels, while reduced serum concentrations of non-esterified fatty acid, β-hydroxybutyric acid, insulin, aspartate aminotransferase, alanine aminotransferase, and haptoglobin during the first month of lactation. CPE supplementation also increased the content of ruminal VFA. Compared to the CON group, the abundance of Prevotellaceae, Methanobacteriaceae, Bacteroidales_RF16_group, and Selenomonadaceae was found increased, while the abundance of Oscillospiraceae, F082, Ruminococcaceae, Christensenellaceae, Muribaculaceae UCG-011, Saccharimonadaceae, Hungateiclostridiaceae, and Spirochaetaceae in the CPE group was found decreased. In adipose tissue, CPE supplementation decreased lipolysis, and inflammatory response, while increased insulin sensitivity. In the liver, CPE supplementation decreased lipid accumulation, increased insulin sensitivity, and upregulated expression of genes involved in gluconeogenesis. Our findings suggest that CPE supplementation during the peripartum period altered rumen microbiota composition and increased ruminal VFA contents, which further improved NEB and lactation performance, alleviated lipolysis and inflammatory response in adipose tissue, reduced lipid accumulation and promoted gluconeogenesis in liver. Thus, CPE might contribute to improve energy metabolism and consequently lactation performance of dairy cows during the transition period.
{"title":"Dietary supplementation with citrus peel extract in transition period improves rumen microbial composition and ameliorates energy metabolism and lactation performance of dairy cows","authors":"Lingxue Ju, Qi Shao, Zhiyuan Fang, Erminio Trevisi, Meng Chen, Yuxiang Song, Wenwen Gao, Lin Lei, Xinwei Li, Guowen Liu, Xiliang Du","doi":"10.1186/s40104-024-01110-8","DOIUrl":"https://doi.org/10.1186/s40104-024-01110-8","url":null,"abstract":"During the transition period, excessive negative energy balance (NEB) lead to metabolic disorders and reduced milk yield. Rumen microbes are responsible for resolving plant material and producing volatile fatty acids (VFA), which are the primary energy source for cows. In this study, we aimed to investigate the effect of citrus peel extract (CPE) supplementation on rumen microbiota composition, energy metabolism and milk performance of peripartum dairy cows. Dairy cows were fed either a basal diet (CON group) or the same basal diet supplemented with CPE via intragastric administration (4 g/d, CPE group) for 6 weeks (3 weeks before and 3 weeks after calving; n = 15 per group). Samples of serum, milk, rumen fluid, adipose tissue, and liver were collected to assess the effects of CPE on rumen microbiota composition, rumen fermentation parameters, milk performance, and energy metabolic status of dairy cows. CPE supplementation led to an increase in milk yield, milk protein and lactose contents, and serum glucose levels, while reduced serum concentrations of non-esterified fatty acid, β-hydroxybutyric acid, insulin, aspartate aminotransferase, alanine aminotransferase, and haptoglobin during the first month of lactation. CPE supplementation also increased the content of ruminal VFA. Compared to the CON group, the abundance of Prevotellaceae, Methanobacteriaceae, Bacteroidales_RF16_group, and Selenomonadaceae was found increased, while the abundance of Oscillospiraceae, F082, Ruminococcaceae, Christensenellaceae, Muribaculaceae UCG-011, Saccharimonadaceae, Hungateiclostridiaceae, and Spirochaetaceae in the CPE group was found decreased. In adipose tissue, CPE supplementation decreased lipolysis, and inflammatory response, while increased insulin sensitivity. In the liver, CPE supplementation decreased lipid accumulation, increased insulin sensitivity, and upregulated expression of genes involved in gluconeogenesis. Our findings suggest that CPE supplementation during the peripartum period altered rumen microbiota composition and increased ruminal VFA contents, which further improved NEB and lactation performance, alleviated lipolysis and inflammatory response in adipose tissue, reduced lipid accumulation and promoted gluconeogenesis in liver. Thus, CPE might contribute to improve energy metabolism and consequently lactation performance of dairy cows during the transition period.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"36 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbiosis. However, it has been challenging to clarify which specific intestinal microbiota and their metabolites play a crucial role in the antidiarrhea process of weaned piglets. In this study, we first observed that piglets with diarrhea exhibited a lower average daily gain and higher diarrhea score, and elevated levels of lipopolysaccharide (LPS) and D-lactate (D-LA) compared to healthy piglets. Subsequently, we analyzed the differences in intestinal microbial composition and metabolite levels between healthy and diarrheal weaned piglets. Diarrheal piglets demonstrated intestinal microbiota dysbiosis, characterized primarily by a higher Firmicutes to Bacteroidota ratio, a deficiency of Lactobacillus amylovorus and Lactobacillus reuteri, and an increased abundance of Bacteroides sp.HF-5287 and Bacteroides thetaiotaomicron. Functional profiling of the gut microbiota based on Kyoto Encyclopedia of Genes and Genomes (KEGG) data was performed, and the results showed that tryptophan metabolism was the most significantly inhibited pathway in piglets with diarrhea. Most tryptophan metabolites were detected at lower concentrations in diarrheal piglets than in healthy piglets. Furthermore, we explored the effects of dietary indole-3-aldehyde (IAld), a key tryptophan metabolite, on intestinal development and gut barrier function in weaned piglets. Supplementation with 100 mg/kg IAld in the diet increased the small intestine index and improved intestinal barrier function by promoting intestinal stem cell (ISC) expansion in piglets. The promotion of ISC expansion by IAld was also confirmed in porcine intestinal organoids. These findings revealed that intestinal microbial tryptophan metabolite IAld alleviates impaired intestinal development by promoting ISC expansion in weaned piglets.
{"title":"The gut microbial metabolite indole-3-aldehyde alleviates impaired intestinal development by promoting intestinal stem cell expansion in weaned piglets","authors":"Jiaqi Zhang, Yahui Chen, Xin Guo, Xuan Li, Ruofan Zhang, Mengting Wang, Weiyun Zhu, Kaifan Yu","doi":"10.1186/s40104-024-01111-7","DOIUrl":"https://doi.org/10.1186/s40104-024-01111-7","url":null,"abstract":"Weaning stress-induced diarrhea is widely recognized as being associated with gut microbiota dysbiosis. However, it has been challenging to clarify which specific intestinal microbiota and their metabolites play a crucial role in the antidiarrhea process of weaned piglets. In this study, we first observed that piglets with diarrhea exhibited a lower average daily gain and higher diarrhea score, and elevated levels of lipopolysaccharide (LPS) and D-lactate (D-LA) compared to healthy piglets. Subsequently, we analyzed the differences in intestinal microbial composition and metabolite levels between healthy and diarrheal weaned piglets. Diarrheal piglets demonstrated intestinal microbiota dysbiosis, characterized primarily by a higher Firmicutes to Bacteroidota ratio, a deficiency of Lactobacillus amylovorus and Lactobacillus reuteri, and an increased abundance of Bacteroides sp.HF-5287 and Bacteroides thetaiotaomicron. Functional profiling of the gut microbiota based on Kyoto Encyclopedia of Genes and Genomes (KEGG) data was performed, and the results showed that tryptophan metabolism was the most significantly inhibited pathway in piglets with diarrhea. Most tryptophan metabolites were detected at lower concentrations in diarrheal piglets than in healthy piglets. Furthermore, we explored the effects of dietary indole-3-aldehyde (IAld), a key tryptophan metabolite, on intestinal development and gut barrier function in weaned piglets. Supplementation with 100 mg/kg IAld in the diet increased the small intestine index and improved intestinal barrier function by promoting intestinal stem cell (ISC) expansion in piglets. The promotion of ISC expansion by IAld was also confirmed in porcine intestinal organoids. These findings revealed that intestinal microbial tryptophan metabolite IAld alleviates impaired intestinal development by promoting ISC expansion in weaned piglets.","PeriodicalId":14928,"journal":{"name":"Journal of Animal Science and Biotechnology","volume":"150 1","pages":""},"PeriodicalIF":7.0,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142597787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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