Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5297
Ali Nasir Hussein, H. J. K. Al-Janabi, J. K. Al-Janabi, Ali R. Shakir Al-Shujairi
One of the most problematic and devastating diseases affecting cucumber production is Fusarium wilt (Cucumis sativus L.). The present study aimed to characterize Fusarium species isolated from three different regions of cucumber fields in Babylon province, Iraq and identify them based on morphological features and phylogenetic analyses. This study showed the presence of Fusarium incarnatum, F. solani and F. oxysporum in cucumber roots. Fusarium species were phylogenetically analyzed based on internal transcribed spacer (ITS) regions. All Fusarium isolates were pathogenic to cucumber cultivars but varied significantly in their growth and pathogenicity toward cucumber seeds during the experiment. F. solani-2 was greater in growth and pathogenicity than all other Fusarium species. In contrast, F. solani-1 was the lowest. The data obtained from morphological and molecular studies sufficiently supported each other, and the phylogenetic trees based on ITS were distinguished. Closely related species and distinctly separated all morphological taxa. These findings are reported for the first time for the cucumber plant in Babylon province. All these ITS sequences showed homologous to those of Fusarium species isolates in the GenBank database with a similarity percentage of 99%. To the best of present knowledge, this is the first molecular record of F. incarnatum on the cucumber plants in Iraq. The study concluded that F. incarnatum was reported for the first time in Iraq and worldwide as a causal agent of wilt disease in cucumber plants. The three species of Fusarium have different pathogenic abilities, highlighting their disease incidence, growth, and pathogenicity.
镰刀菌枯萎病（Cucumis sativus L.）是影响黄瓜生产的最棘手和最具破坏性的病害之一。本研究旨在描述从伊拉克巴比伦省三个不同地区的黄瓜田中分离出来的镰刀菌种的特征，并根据形态特征和系统发育分析对其进行鉴定。研究结果表明，黄瓜根部存在 Fusarium incarnatum、F. solani 和 F. oxysporum。根据内部转录间隔区（ITS）对镰刀菌进行了系统发育分析。所有镰刀菌分离物对黄瓜栽培品种都有致病性，但在实验过程中，它们对黄瓜种子的生长和致病性有显著差异。 F.solani-2的生长和致病性高于所有其他镰刀菌种。相比之下，F. solani-1 的致病力最低。形态学研究和分子研究获得的数据相互充分支持，基于 ITS 的系统发生树被区分开来。所有形态类群中都有近缘种和明显的分离种。这些发现是巴比伦省黄瓜植物的首次报道。所有这些 ITS 序列都与 GenBank 数据库中的镰刀菌种分离物具有同源性，相似度高达 99%。据目前所知，这是伊拉克黄瓜植物上首次出现 F. incarnatum 的分子记录。该研究得出结论，在伊拉克和世界范围内首次报道了 F. incarnatum 是黄瓜枯萎病的病原菌。这三种镰刀菌具有不同的致病能力，突出了它们的发病率、生长和致病性。
{"title":"Identification and characterizations of a few species of Fusarium infecting cucumber in greenhouse conditions","authors":"Ali Nasir Hussein, H. J. K. Al-Janabi, J. K. Al-Janabi, Ali R. Shakir Al-Shujairi","doi":"10.31018/jans.v16i1.5297","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5297","url":null,"abstract":"One of the most problematic and devastating diseases affecting cucumber production is Fusarium wilt (Cucumis sativus L.). The present study aimed to characterize Fusarium species isolated from three different regions of cucumber fields in Babylon province, Iraq and identify them based on morphological features and phylogenetic analyses. This study showed the presence of Fusarium incarnatum, F. solani and F. oxysporum in cucumber roots. Fusarium species were phylogenetically analyzed based on internal transcribed spacer (ITS) regions. All Fusarium isolates were pathogenic to cucumber cultivars but varied significantly in their growth and pathogenicity toward cucumber seeds during the experiment. F. solani-2 was greater in growth and pathogenicity than all other Fusarium species. In contrast, F. solani-1 was the lowest. The data obtained from morphological and molecular studies sufficiently supported each other, and the phylogenetic trees based on ITS were distinguished. Closely related species and distinctly separated all morphological taxa. These findings are reported for the first time for the cucumber plant in Babylon province. All these ITS sequences showed homologous to those of Fusarium species isolates in the GenBank database with a similarity percentage of 99%. To the best of present knowledge, this is the first molecular record of F. incarnatum on the cucumber plants in Iraq. The study concluded that F. incarnatum was reported for the first time in Iraq and worldwide as a causal agent of wilt disease in cucumber plants. The three species of Fusarium have different pathogenic abilities, highlighting their disease incidence, growth, and pathogenicity. \u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5316
Agus Suharyanto, Alwafi Pujiraharjo, M. T. Iqbal, Article Info
An increase in population increases the rate of urbanization. This results in changes in land cover from vegetation to artificial material. As a result, much of the land surface reflects the sun's energy. Consequently, this increases the surface temperature of the land. An increase in land surface temperature (LST) will increase the intensity of rainfall. Therefore, the present study aimed to investigate the relationship between the increase in LST and rainfall intensity. Changes in land cover can be detected by normalized difference vegetation index (NDVI) and normalized difference built-up index (NDBI) parameters. Landsat satellite imagery was used to detect NDVI, NDBI, and LST. Image processing was done for imageries scanned in 1995, 2015, 2017, and 2021. Two areas in the East Java Province of Indonesia, namely Malang City and Pasuruan Area, were selected. The daily rainfall intensity data were collected from related rainfall stations in the same year. The Mononobe method was applied to analyze hourly and minute rainfall intensity. IDF curves were drawn from the analyzed results. The relationship between both parameters was analyzed by comparing the LST and hourly rainfall intensity from the IDF curve. The studied results showed that the maximum temperature increase from 1995 to 2021 for the Malang City and Pasuruan Area was 2.60 C and 7.60 C, respectively. For rain, the maximum rainfall intensity increased by 58 mm for Malang City and 18 mm for the Pasuruan Area. LST and rainfall intensity change trends of the two areas had a positive coefficient of regression. The findings can be used to predict the rainfall intensity and floods based on the LST data.
{"title":"Influence of vegetation index to the rainfall intensity in Pasuruan Area, East Java Province, Indonesia","authors":"Agus Suharyanto, Alwafi Pujiraharjo, M. T. Iqbal, Article Info","doi":"10.31018/jans.v16i1.5316","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5316","url":null,"abstract":"An increase in population increases the rate of urbanization. This results in changes in land cover from vegetation to artificial material. As a result, much of the land surface reflects the sun's energy. Consequently, this increases the surface temperature of the land. An increase in land surface temperature (LST) will increase the intensity of rainfall. Therefore, the present study aimed to investigate the relationship between the increase in LST and rainfall intensity. Changes in land cover can be detected by normalized difference vegetation index (NDVI) and normalized difference built-up index (NDBI) parameters. Landsat satellite imagery was used to detect NDVI, NDBI, and LST. Image processing was done for imageries scanned in 1995, 2015, 2017, and 2021. Two areas in the East Java Province of Indonesia, namely Malang City and Pasuruan Area, were selected. The daily rainfall intensity data were collected from related rainfall stations in the same year. The Mononobe method was applied to analyze hourly and minute rainfall intensity. IDF curves were drawn from the analyzed results. The relationship between both parameters was analyzed by comparing the LST and hourly rainfall intensity from the IDF curve. The studied results showed that the maximum temperature increase from 1995 to 2021 for the Malang City and Pasuruan Area was 2.60 C and 7.60 C, respectively. For rain, the maximum rainfall intensity increased by 58 mm for Malang City and 18 mm for the Pasuruan Area. LST and rainfall intensity change trends of the two areas had a positive coefficient of regression. The findings can be used to predict the rainfall intensity and floods based on the LST data.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Using chemical defoliants to remove cotton leaves represents a groundbreaking shift in cotton cultivation. The mechanization of cotton harvest is increasing, but a substantial amount of foliage that remains on the plant even at maturity is the major barrier for mechanical harvest. Properly completing mechanical and manual harvests requires artificial leaf detachment through defoliants. Still there is no proper defoliant concentration, application times and mechanism of action available. Therefore, the present study aimed to find an effective defoliant and application time to enhance mechanical harvest efficiency, along with a clear description of the mechanism of actions in cotton CO17 (Gossypium hirsutum). The field experiment was conducted during the year 2019-20 and used five concentrations of Thidiazuron defoliant (100, 150, 200, 250 and 300ppm) and Ethephon@0.5% (T2) in cotton variety CO17 to study the physiological, biochemical and hormonal responses at 120, 127 and 134 days after sowing. As a result, the concentrations of plant growth hormones, indole-3 acetic acid (4.9 fold), zeatin (32.7%) and gibberellic acid (7 fold) reduced. In contrast, abscisic acid (48.6%), jasmonic acid (34.9%), salicylic acid (2.15 fold) increased in the T7- Thidiazuron + Diuron (300 ppm) treatment followed by T5-Thidiazuron + Diuron (200 ppm). Additionally, the antioxidant enzymes ascorbate peroxidase, peroxidase, catalase, superoxide dismutase, cellulase in leaves, petiole and bolls were decreased due to defoliant T5- Thidiazuron + Diuron (200 ppm) followed by T7-Thidiazuron + Diuron (300 ppm), indicating that the hormone concentration, antioxidative and hydrolytic enzymes are ruled out and forces the defoliation process.
{"title":"Dissecting the biochemical and hormonal changes of thidiazuron on defoliation of cotton CO17 (Gossypium hirsutum) to enhance mechanical harvest efficiency","authors":"C. Perumal, Ashok Subiramaniyan, Ashokkumar Natarajan, Rajeshkumar Arumugam, Ajaykumar Ramasamy, Ramadass Sivalingam, Karpagavalli Sivasubramanian","doi":"10.31018/jans.v16i1.4860","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4860","url":null,"abstract":"Using chemical defoliants to remove cotton leaves represents a groundbreaking shift in cotton cultivation. The mechanization of cotton harvest is increasing, but a substantial amount of foliage that remains on the plant even at maturity is the major barrier for mechanical harvest. Properly completing mechanical and manual harvests requires artificial leaf detachment through defoliants. Still there is no proper defoliant concentration, application times and mechanism of action available. Therefore, the present study aimed to find an effective defoliant and application time to enhance mechanical harvest efficiency, along with a clear description of the mechanism of actions in cotton CO17 (Gossypium hirsutum). The field experiment was conducted during the year 2019-20 and used five concentrations of Thidiazuron defoliant (100, 150, 200, 250 and 300ppm) and Ethephon@0.5% (T2) in cotton variety CO17 to study the physiological, biochemical and hormonal responses at 120, 127 and 134 days after sowing. As a result, the concentrations of plant growth hormones, indole-3 acetic acid (4.9 fold), zeatin (32.7%) and gibberellic acid (7 fold) reduced. In contrast, abscisic acid (48.6%), jasmonic acid (34.9%), salicylic acid (2.15 fold) increased in the T7- Thidiazuron + Diuron (300 ppm) treatment followed by T5-Thidiazuron + Diuron (200 ppm). Additionally, the antioxidant enzymes ascorbate peroxidase, peroxidase, catalase, superoxide dismutase, cellulase in leaves, petiole and bolls were decreased due to defoliant T5- Thidiazuron + Diuron (200 ppm) followed by T7-Thidiazuron + Diuron (300 ppm), indicating that the hormone concentration, antioxidative and hydrolytic enzymes are ruled out and forces the defoliation process.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5286
P. Abhilash, Nilesh Talekar, I. Delvadiya, S. Anvesh, Article Info
Evaluating genetic diversity simplifies the identification of superior genotypes, facilitating the development of high-yielding, resilient to climatic conditions and promoting effective crop improvement. The present study aimed to examine the divergence, correlation and path analysis across 55 okra (Abelmoschus esculentus (L.) germplasms for 17 traits during the summer season (March- July) of 2022. The experiment utilized randomized complete block design with three replications conducted in Phagwara, Punjab. Analysis of variance suggested a sufficient amount of genetic variation was found among all genotypes. Using Mahalanobis D2 analysis, the samples were classified into seven clusters, the largest being cluster I, composing 34 germplasm. Only one germplasm was found in Clusters II, IV, V, VI, and VII. The inter-cluster distance was highest between clusters II and VII, whereas the intra-cluster distance was greatest in Cluster III. The number of fruits per plant had the highest percent contribution to the divergence, accounting for 49.63%. At both phenotypic and genotypic levels, there was a strong positive correlation (+) observed between fruit yield and various characteristics, including plant height, fruit length, number of fruits per plant, number of marketable fruits per plant, average fruit weight, and the number of pickings. Genotypic path analysis revealed that characteristics such as the first flowering node, days to first flowering, days to 50% flowering, plant height, inter-nodal length, number of nodes per plant, number of fruits per plant, marketable fruits per plant, and average fruit weight exhibited a positive and direct effect on fruit yield. When selecting this trait to improve yield in okra through breeding, it is essential to focus on specific characteristics that directly contribute to higher production. This research will help resilient okra varieties understand yield-influencing factors in Punjab environmental conditions.
{"title":"Exploring multivariate associations of yield and yield-associated traits in okra (Abelmoschus esculentus (L.) Moench) accessions in the Northwestern Region of India","authors":"P. Abhilash, Nilesh Talekar, I. Delvadiya, S. Anvesh, Article Info","doi":"10.31018/jans.v16i1.5286","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5286","url":null,"abstract":"Evaluating genetic diversity simplifies the identification of superior genotypes, facilitating the development of high-yielding, resilient to climatic conditions and promoting effective crop improvement. The present study aimed to examine the divergence, correlation and path analysis across 55 okra (Abelmoschus esculentus (L.) germplasms for 17 traits during the summer season (March- July) of 2022. The experiment utilized randomized complete block design with three replications conducted in Phagwara, Punjab. Analysis of variance suggested a sufficient amount of genetic variation was found among all genotypes. Using Mahalanobis D2 analysis, the samples were classified into seven clusters, the largest being cluster I, composing 34 germplasm. Only one germplasm was found in Clusters II, IV, V, VI, and VII. The inter-cluster distance was highest between clusters II and VII, whereas the intra-cluster distance was greatest in Cluster III. The number of fruits per plant had the highest percent contribution to the divergence, accounting for 49.63%. At both phenotypic and genotypic levels, there was a strong positive correlation (+) observed between fruit yield and various characteristics, including plant height, fruit length, number of fruits per plant, number of marketable fruits per plant, average fruit weight, and the number of pickings. Genotypic path analysis revealed that characteristics such as the first flowering node, days to first flowering, days to 50% flowering, plant height, inter-nodal length, number of nodes per plant, number of fruits per plant, marketable fruits per plant, and average fruit weight exhibited a positive and direct effect on fruit yield. When selecting this trait to improve yield in okra through breeding, it is essential to focus on specific characteristics that directly contribute to higher production. This research will help resilient okra varieties understand yield-influencing factors in Punjab environmental conditions.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5347
Anupama Shukla
The Laboulbeniales are a group of lesser-known fungi. They occur as ectoparasites on the exoskeleton of various arthropod species, mainly beetles, cockroaches, ants, dipterans and a few millipede genera appearing as hairs/bristles coming out of their cuticle. They are small, non-mycelial fungi forming compact multicellular thalli, starting from a two-celled sheathed ascospore which continues to envelop the mature thallus. The fungi, being obligate, spend their entire life cycle on their insect hosts. The present study attempted to locate the Laboulbeniales fungi in the common insects of India, isolate them and identify them based on morphology. Different species of insects; Carabid beetles - Stenolophus sp., Bembidion sp., Black ants - Camponotus sp., and Earwigs - Labidura riparia; were collected from different habitats of damp garden soil, river banks, light sources and entomological collection museum situated as different sites New Delhi. The insects were scrutinized for the presence of fungi through light and scanning electron microscopy. The morphological features of each fungus were recorded, and the species were characterized. The studies showed the presence of Laboulbenia anaplogenii and L. stenolophi on the legs of Stenolophus sp.; L. egens on Bembidion sp. legs; L. camponoti on the wings of black ants and Dimeromyces anisolabis on the legs of earwigs. The identification was made primarily based on the size, shape and structure of basal foot, receptacles, antheridia and foot of the thallus or it’s remnant scar found on the insect cuticle. This is the first Indian report of conducting scanning imaging of the Laboulbeniales fungi.
Laboulbeniales 是一组鲜为人知的真菌。它们作为外寄生虫出现在各种节肢动物的外骨骼上，主要是甲虫、蟑螂、蚂蚁、双翅目动物和一些千足类，表现为从角质层中伸出的毛发/绒毛。它们是小型的非菌丝真菌，形成紧密的多细胞菌柄，从两室的有鞘子囊孢子开始，一直到成熟的菌丝体。这种真菌必须在昆虫宿主身上度过整个生命周期。本研究试图在印度常见的昆虫中找到拉布贝氏真菌，分离它们并根据形态鉴定它们。研究人员从新德里不同地点的潮湿花园土壤、河岸、光源和昆虫学收藏博物馆等不同栖息地收集了不同种类的昆虫：食肉甲虫--Stenolophus sp.、Bembidion sp.、黑蚂蚁--Camponotus sp.和蠼--Labidura riparia。通过光学显微镜和扫描电子显微镜检查昆虫体内是否存在真菌。记录了每种真菌的形态特征，并对其种类进行了鉴定。研究表明，在 Stenolophus sp.的腿上有 Laboulbenia anaplogenii 和 L. stenolophi；在 Bembidion sp.的腿上有 L. egens；在黑蚂蚁的翅膀上有 L. camponoti；在蠼螋的腿上有 Dimeromyces anisolabis。鉴定的主要依据是基脚、花托、花药和菌褶脚的大小、形状和结构，或在昆虫角质层上发现的残留疤痕。这是印度第一份对拉布贝菌进行扫描成像的报告。
{"title":"Species of Laboulbeniales fungi parasitic on some common Indian insects: Isolation, visualization and characterization by Scanning Electron Micrographs (SEM)","authors":"Anupama Shukla","doi":"10.31018/jans.v16i1.5347","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5347","url":null,"abstract":"The Laboulbeniales are a group of lesser-known fungi. They occur as ectoparasites on the exoskeleton of various arthropod species, mainly beetles, cockroaches, ants, dipterans and a few millipede genera appearing as hairs/bristles coming out of their cuticle. They are small, non-mycelial fungi forming compact multicellular thalli, starting from a two-celled sheathed ascospore which continues to envelop the mature thallus. The fungi, being obligate, spend their entire life cycle on their insect hosts. The present study attempted to locate the Laboulbeniales fungi in the common insects of India, isolate them and identify them based on morphology. Different species of insects; Carabid beetles - Stenolophus sp., Bembidion sp., Black ants - Camponotus sp., and Earwigs - Labidura riparia; were collected from different habitats of damp garden soil, river banks, light sources and entomological collection museum situated as different sites New Delhi. The insects were scrutinized for the presence of fungi through light and scanning electron microscopy. The morphological features of each fungus were recorded, and the species were characterized. The studies showed the presence of Laboulbenia anaplogenii and L. stenolophi on the legs of Stenolophus sp.; L. egens on Bembidion sp. legs; L. camponoti on the wings of black ants and Dimeromyces anisolabis on the legs of earwigs. The identification was made primarily based on the size, shape and structure of basal foot, receptacles, antheridia and foot of the thallus or it’s remnant scar found on the insect cuticle. This is the first Indian report of conducting scanning imaging of the Laboulbeniales fungi.","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5207
Djohan Djohan
Cartilage and bones (CB) are primary organs for lead accumulation in domestic animals, but very few studies have addressed the occurrence of lead in both organs. CBs, especially from goat and chicken, although classified as non-edible food, are still being consumed by many local communities globally. Male goat and rooster were bought in traditional markets in Yogyakarta, Indonesia. Lead in CBs of humerus, radius, femur, and tibia (HRFT) were extracted by aqua regia digestible method and measured by Atomic Absorption Spectrophotometry. A set of data ratios, generated by data ratio analysis (DRA), namely concentration ratio (CR), amounts ratio (AR), variation ratio (VR), coefficient of variation ratio (CVR), joint probability (JP) and conditional probability (CP) based on the AR and CR, were determined to characterize the lead exposure in CB. The means of lead concentrations in CB in goat were 4.9 and 5.2 µg.g-1, and those in chicken were 5.00 and 5.20 µg.g-1, respectively. The CRs in goats and chickens were 0.95 and 0.96, and the ARs in goats and chickens were 1.34 and 1.16, respectively. A high linear relationship was found between Ln VR and Ln CVR, and the JP and CP supported decision-making on CB consumption. By targeting CB as primary organs for lead accumulation, considering its potential exposure for many communities, and comparing its further uses as animal feed, the DRA in this study is useful to provide broadened comparability in bioaccumulation studies.
{"title":"Application of data ratio analysis of lead accumulation in cartilage and bones of goats and chickens in Yogyakarta, Indonesia","authors":"Djohan Djohan","doi":"10.31018/jans.v16i1.5207","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5207","url":null,"abstract":"Cartilage and bones (CB) are primary organs for lead accumulation in domestic animals, but very few studies have addressed the occurrence of lead in both organs. CBs, especially from goat and chicken, although classified as non-edible food, are still being consumed by many local communities globally. Male goat and rooster were bought in traditional markets in Yogyakarta, Indonesia. Lead in CBs of humerus, radius, femur, and tibia (HRFT) were extracted by aqua regia digestible method and measured by Atomic Absorption Spectrophotometry. A set of data ratios, generated by data ratio analysis (DRA), namely concentration ratio (CR), amounts ratio (AR), variation ratio (VR), coefficient of variation ratio (CVR), joint probability (JP) and conditional probability (CP) based on the AR and CR, were determined to characterize the lead exposure in CB. The means of lead concentrations in CB in goat were 4.9 and 5.2 µg.g-1, and those in chicken were 5.00 and 5.20 µg.g-1, respectively. The CRs in goats and chickens were 0.95 and 0.96, and the ARs in goats and chickens were 1.34 and 1.16, respectively. A high linear relationship was found between Ln VR and Ln CVR, and the JP and CP supported decision-making on CB consumption. By targeting CB as primary organs for lead accumulation, considering its potential exposure for many communities, and comparing its further uses as animal feed, the DRA in this study is useful to provide broadened comparability in bioaccumulation studies.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5218
Vinay Kumar, P. K. R. Kumar, Y. T. R. Babu, Article Info
Leaf spot disease caused by Alternaria perpunctulata (E.G. Simmons) poses a significant threat to Alternanthera philoxeroides (Mart.) Griseb, commonly known as alligator weed and Ceylon Spinach, contributes to persistent weed proliferation in Indian paddy fields. This study focuses on the molecular identification and phylogenetic analysis of the Alternaria perpunctulata strain GVKNSV7, which is responsible for leaf spot disease on A. philoxeroides. Diseased plant leaves were collected from Kakumanu Mandal, Guntur, and local vegetable markets of Visakhapatnam, Andhra Pradesh, India, in December 2021, revealing symptomatic samples with pink to reddish spots on A. philoxeroides leaves. Employing a comprehensive approach that included morphological assessment, pathogenicity testing, and ITS region sequencing, the isolated fungus was conclusively identified as A. perpunctulata with a high sequence similarity of 98.54%. Molecular comparison with sequences in the NCBI database further validated this identification. The resulting genetic sequence, formally catalogued as "Alternaria perpunctulata GVKNSV7," with the GenBank accession number OQ073752, has been successfully submitted to the NCBI database. This study emphasized the utility of ITS sequencing and molecular analyses for accurately identifying and documenting emerging pathogens. Such documentation provides essential insights for developing future bioherbicides and effective weed management strategies. Furthermore, it highlights the potential use of A. philoxeroides as a leafy vegetable, contributing to food security and agricultural sustainability. Overall, this research contributes to understanding Alternaria perpunctulata and its implications for crop health and management practices.
由Alternaria perpunctulata (E.G. Simmons) 引起的叶斑病对Alternanthera philoxeroides (Mart.) Griseb（俗称鳄鱼草和锡兰菠菜）构成了严重威胁，导致印度稻田杂草持续泛滥。本研究的重点是对造成 A. philoxeroides 叶斑病的 Alternaria perpunctulata 菌株 GVKNSV7 进行分子鉴定和系统发育分析。2021 年 12 月，研究人员从印度安得拉邦贡图尔的卡库马努曼达尔（Kakumanu Mandal）和维萨卡帕特南的当地蔬菜市场采集了病株叶片，发现有症状的样本中，茴芹叶片上出现粉红色至淡红色斑点。通过形态学评估、致病性测试和 ITS 区域测序等综合方法，最终确定分离出的真菌为 A. perpunctulata，其序列相似度高达 98.54%。与 NCBI 数据库中的序列进行的分子比较进一步验证了这一鉴定结果。由此产生的基因序列正式编目为 "Alternaria perpunctulata GVKNSV7"，GenBank登录号为 OQ073752，并已成功提交至 NCBI 数据库。这项研究强调了 ITS 测序和分子分析在准确识别和记录新兴病原体方面的作用。这种记录为开发未来的生物除草剂和有效的杂草管理策略提供了重要依据。此外，它还强调了 A. philoxeroides 作为叶菜的潜在用途，有助于粮食安全和农业可持续发展。总之，这项研究有助于了解Alternaria perpunctulata及其对作物健康和管理实践的影响。
{"title":"Molecular identification and phylogenetic analysis of Alternaria perpunctulata GVKNSV7 causing leaf spot disease on Alternanthera philoxeroides (Mart.) Griseb : A first report in India","authors":"Vinay Kumar, P. K. R. Kumar, Y. T. R. Babu, Article Info","doi":"10.31018/jans.v16i1.5218","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5218","url":null,"abstract":"Leaf spot disease caused by Alternaria perpunctulata (E.G. Simmons) poses a significant threat to Alternanthera philoxeroides (Mart.) Griseb, commonly known as alligator weed and Ceylon Spinach, contributes to persistent weed proliferation in Indian paddy fields. This study focuses on the molecular identification and phylogenetic analysis of the Alternaria perpunctulata strain GVKNSV7, which is responsible for leaf spot disease on A. philoxeroides. Diseased plant leaves were collected from Kakumanu Mandal, Guntur, and local vegetable markets of Visakhapatnam, Andhra Pradesh, India, in December 2021, revealing symptomatic samples with pink to reddish spots on A. philoxeroides leaves. Employing a comprehensive approach that included morphological assessment, pathogenicity testing, and ITS region sequencing, the isolated fungus was conclusively identified as A. perpunctulata with a high sequence similarity of 98.54%. Molecular comparison with sequences in the NCBI database further validated this identification. The resulting genetic sequence, formally catalogued as \"Alternaria perpunctulata GVKNSV7,\" with the GenBank accession number OQ073752, has been successfully submitted to the NCBI database. This study emphasized the utility of ITS sequencing and molecular analyses for accurately identifying and documenting emerging pathogens. Such documentation provides essential insights for developing future bioherbicides and effective weed management strategies. Furthermore, it highlights the potential use of A. philoxeroides as a leafy vegetable, contributing to food security and agricultural sustainability. Overall, this research contributes to understanding Alternaria perpunctulata and its implications for crop health and management practices.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5243
Manish Bakshi, Pallvi Verma, Anis Ahmad Mirza, S. Singh, Suhel Mehndi, Article Info
Foliar spraying of nanoparticles (NPs) improves the absorption of plant nutrient application compared to traditional soil–root application, and it also enhances the yield and quality of fruits. The present study aimed to evaluate the qualitative effects of foliar sprays of two concentrations of nano-zinc and nano-copper (40 ppm, 60 ppm; 20 ppm, 30 ppm respectively), in comparison to ZnSo4 (recommended by, Punjab Agriculture University, Ludhiana) and control (foliar spray of water) on the guava crop (var. Allahabad Safeda). The experiment was conducted at Lovely Professional University Research farm, Phagwara, Jalandhar (Punjab) by applying a simple randomized block design, with ten treatments applied as T1: control, T2: nano-Zn1, T3: nano-Zn2, T4: nano-Cu1, T5: nano-Cu2, T6: nano-Zn1+ nano-Cu1, T7: nano-Zn1+ nano-Cu2, T8: nano-Zn2 + nano-Cu1, T9: nano-Zn2 + nano-Cu2, T10: ZnSO4 (PAU recommendation) in three replications. The treatments were sprayed two times, first at the flowering stage and second when the fruit reached pea size. The nutrient spray increases the concentration of nutrients in the leaves while also affecting the biochemical parameters. The performance for total soluble solids (9.89°B), total sugars (8.74%), titratable acidity (0.98%), antioxidants (7.49%), firmness (5.71kg/cm2), non-reducing sugars (3.32%), Vitamin-C (268.90mg/100g pulp), pectin content (2.13%), reducing sugars (5.46%), and TSS/acid ratio (10.06) was superior with the application of nano-Zn2 + nano-Cu2 (T9). The application of nano-micronutrients (zinc and copper) in combination is favorable for the quality of guava fruit (Allahabad Safeda).
{"title":"Impact of foliar spray of nano-Zn and nano-Cu on biochemical characteristics of guava cv. Allahabad Safeda","authors":"Manish Bakshi, Pallvi Verma, Anis Ahmad Mirza, S. Singh, Suhel Mehndi, Article Info","doi":"10.31018/jans.v16i1.5243","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5243","url":null,"abstract":"Foliar spraying of nanoparticles (NPs) improves the absorption of plant nutrient application compared to traditional soil–root application, and it also enhances the yield and quality of fruits. The present study aimed to evaluate the qualitative effects of foliar sprays of two concentrations of nano-zinc and nano-copper (40 ppm, 60 ppm; 20 ppm, 30 ppm respectively), in comparison to ZnSo4 (recommended by, Punjab Agriculture University, Ludhiana) and control (foliar spray of water) on the guava crop (var. Allahabad Safeda). The experiment was conducted at Lovely Professional University Research farm, Phagwara, Jalandhar (Punjab) by applying a simple randomized block design, with ten treatments applied as T1: control, T2: nano-Zn1, T3: nano-Zn2, T4: nano-Cu1, T5: nano-Cu2, T6: nano-Zn1+ nano-Cu1, T7: nano-Zn1+ nano-Cu2, T8: nano-Zn2 + nano-Cu1, T9: nano-Zn2 + nano-Cu2, T10: ZnSO4 (PAU recommendation) in three replications. The treatments were sprayed two times, first at the flowering stage and second when the fruit reached pea size. The nutrient spray increases the concentration of nutrients in the leaves while also affecting the biochemical parameters. The performance for total soluble solids (9.89°B), total sugars (8.74%), titratable acidity (0.98%), antioxidants (7.49%), firmness (5.71kg/cm2), non-reducing sugars (3.32%), Vitamin-C (268.90mg/100g pulp), pectin content (2.13%), reducing sugars (5.46%), and TSS/acid ratio (10.06) was superior with the application of nano-Zn2 + nano-Cu2 (T9). The application of nano-micronutrients (zinc and copper) in combination is favorable for the quality of guava fruit (Allahabad Safeda). \u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Over the past few years, the biological properties of garlic have been utilized as an attractive natural alternative to many therapeutic drugs. The biological effects of garlic have been ascribed to organosulfur compounds, secondary metabolites derived from amino acids. The present study aimed to investigate the extracts of a novel garlic variety (Hisar garlic 17) after processing it as fresh, dry, heated, and aged in different solvents and then analyzed with highly sensitive and rapid technique i.e., High resolution liquid chromatograph mass spectrometer quadrupole time of flight (HR-LCMS-QTOF) Mass spectrometer to study the amino acids and organosulfur compounds. 47 amino acids and 11 organosulfur compounds were detected out of which 8 organosulfur compounds were found as secondary metabolites of amino acids. Among the 22 crucial α-amino acids, garlic extracts revealed the presence of 18 amino acids, either in their native state or associated with various complex compounds. The study reported major organosulfur compounds, including Alliin, S-allyl cysteine, S-methyl cysteine, N-gamma-Glutamyl-S-allylcysteine, and 2-Propenyl 1-(2- propenylsulfinyl)propyl disulfide. This study demonstrated that HG17 garlic is abundant in amino acids and organosulfur compounds, suggesting its potential utilization as a supplement in nutraceuticals.
{"title":"Profiling of organosulfur compounds and amino acids in novel variety of Allium sativum (Hisar garlic 17) by HR-LCMS-QTOF","authors":"Monika Monika, Anita Dua, Sandeep Kumar, Sanjay Sharma, S. Gupta, Ashwani Mittal","doi":"10.31018/jans.v16i1.5386","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5386","url":null,"abstract":"Over the past few years, the biological properties of garlic have been utilized as an attractive natural alternative to many therapeutic drugs. The biological effects of garlic have been ascribed to organosulfur compounds, secondary metabolites derived from amino acids. The present study aimed to investigate the extracts of a novel garlic variety (Hisar garlic 17) after processing it as fresh, dry, heated, and aged in different solvents and then analyzed with highly sensitive and rapid technique i.e., High resolution liquid chromatograph mass spectrometer quadrupole time of flight (HR-LCMS-QTOF) Mass spectrometer to study the amino acids and organosulfur compounds. 47 amino acids and 11 organosulfur compounds were detected out of which 8 organosulfur compounds were found as secondary metabolites of amino acids. Among the 22 crucial α-amino acids, garlic extracts revealed the presence of 18 amino acids, either in their native state or associated with various complex compounds. The study reported major organosulfur compounds, including Alliin, S-allyl cysteine, S-methyl cysteine, N-gamma-Glutamyl-S-allylcysteine, and 2-Propenyl 1-(2- propenylsulfinyl)propyl disulfide. This study demonstrated that HG17 garlic is abundant in amino acids and organosulfur compounds, suggesting its potential utilization as a supplement in nutraceuticals.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5303
Anita Narang, Surinder Kaur, Anupama Shukla
Major problems encountered in establishing axenic cultures are contamination and browning of explants and media. Contamination is initiated in explants excised from soil-borne tissues or adult trees. Several sterilizing agents (Tween 20, chlorine water, silver nitrate, mercuric chloride, etc.) or a wash in antibiotic solution are recommended to reduce this. . Establishing in vitro cultures of Acacia nilotica old tree nodal explants was a serious problem. The present study was undertaken to create a suitable protocol for in vitro micropropagation of nodal explants of Acacia nilotica subsp. indica. Contamination was controlled by sterilizing the explants by washing in polysan (5%, v/v along with 500mg/l PVP), thorough washing under tap water, 70% alcohol treatment and 0.1% HgCl2 treatment. Plant tissues release phenolic substances through their cut ends, which turn the media dark brown and toxic. Addition of various antioxidants or/and transfer of explants to fresh medium twice or thrice, at a few days’ intervals may overcome the problem. Collection of explants in antioxidant solution and a wash in antioxidant solution prior to inoculation was helpful in reducing the phenolic exudation in the present investigations. The addition of antioxidant (citric acid) to the medium also checked browning to some extent. The rate of infection and browning of explants varied in different seasons, the maximum being during winters and the minimum during summers of 2019. This was inversely related to the morphogenic response of explants i.e. maximum caulogenesis occurred in vitro during July (6.3±1.4 shoots per explant). The in vitro raised shoots showed 100% rooting on 2mg/l IBA augmented Nitsch’s (N) medium.
{"title":"Seasonal variation in contamination and browning of Acacia nilotica nodal explants in vitro","authors":"Anita Narang, Surinder Kaur, Anupama Shukla","doi":"10.31018/jans.v16i1.5303","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5303","url":null,"abstract":"Major problems encountered in establishing axenic cultures are contamination and browning of explants and media. Contamination is initiated in explants excised from soil-borne tissues or adult trees. Several sterilizing agents (Tween 20, chlorine water, silver nitrate, mercuric chloride, etc.) or a wash in antibiotic solution are recommended to reduce this. . Establishing in vitro cultures of Acacia nilotica old tree nodal explants was a serious problem. The present study was undertaken to create a suitable protocol for in vitro micropropagation of nodal explants of Acacia nilotica subsp. indica. Contamination was controlled by sterilizing the explants by washing in polysan (5%, v/v along with 500mg/l PVP), thorough washing under tap water, 70% alcohol treatment and 0.1% HgCl2 treatment. Plant tissues release phenolic substances through their cut ends, which turn the media dark brown and toxic. Addition of various antioxidants or/and transfer of explants to fresh medium twice or thrice, at a few days’ intervals may overcome the problem. Collection of explants in antioxidant solution and a wash in antioxidant solution prior to inoculation was helpful in reducing the phenolic exudation in the present investigations. The addition of antioxidant (citric acid) to the medium also checked browning to some extent. The rate of infection and browning of explants varied in different seasons, the maximum being during winters and the minimum during summers of 2019. This was inversely related to the morphogenic response of explants i.e. maximum caulogenesis occurred in vitro during July (6.3±1.4 shoots per explant). The in vitro raised shoots showed 100% rooting on 2mg/l IBA augmented Nitsch’s (N) medium.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 42","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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