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Evaluation on the development of soil fertility gradients with nutrient exhaustive crop (Sorghum bicolor) regard to N, P, and K in Inceptisols in semi-arid regions of Tamil Nadu 泰米尔纳德邦半干旱区籼稻(高粱)对N、P、K养分穷尽的土壤肥力梯度发展评价
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4788
Maragani Vamshi, S. Maragatham, R. Santhi, M. K. Kalarani, A. Sankari, M. Gopalakrishnan, R. Rajeswari
Soil test crop response (STCR's) soil fertility gradient approach is based on the idea that complex treatments are superimposed in a field to obtain crop responses for correlating with soil test values that are artificially created by differential fertiliser treatments prior to conducting the regular experiment, thereby providing a scientific basis for balanced fertilisation between applied and available forms of nutrients. The present study aimed to develop the fertility gradient with sorghum as test crop in the field concerning N, P, and K and also to evaluate its impact on sorghum nutrient uptake, and soil fertility. The experimental field was split into three equal strips. Strips I, II, and III each received three graded levels of fertiliser N (nitrogen), P2O5 (phosphorus pentoxide), and K2O (potassium oxide) as urea, single super phosphate, and muriate of potash, respectively. The green fodder yield of sorghum recorded at harvest in strip I, II & III was 16.4, 23.4 and 28.2 t ha-1, respectively. Whereas post-harvest soil available nitrogen in strip I, II & III was 155, 190 & 214 kg ha-1 respectively, for available phosphorus 12.5, 23.2 & 31.8 kg ha-1 respectively and for available potassium it is 332, 370 & 396 kg ha-1 respectively. Wide variations in green fodder yield and soil fertility were observed among the strips, establishing the influence of graded amounts of fertiliser treatment on these parameters and the formation of a soil fertility gradient.
土壤试验作物反应(STCR)土壤肥力梯度方法是基于这样一种想法,即在田地中叠加复杂的处理,以获得作物反应,并与土壤试验值相关联,这些土壤试验值是在进行常规试验之前通过差异施肥人工产生的,从而为施用和可利用的养分形式之间的平衡施肥提供科学依据。本研究以高粱为试验作物,在田间建立了氮、磷、钾的肥力梯度,并评价其对高粱养分吸收和土壤肥力的影响。试验田被分成三等长条。条I、条II和条III分别以尿素、单一过磷酸钾和钾肥盐三个等级分别施用N(氮)、P2O5(五氧化二磷)和K2O(氧化钾)。1、2、3条收获期高粱青饲料产量记录III分别为16.4、23.4和28.2 t ha-1。收获后I、II条土壤有效氮;III为155,190 &速效磷分别为12.5、23.2和22.5 kg hm -1;速效钾分别为332、370和31.8 kg hm -1;分别为396 kg ha-1。绿饲料产量和土壤肥力在条带之间存在很大差异,确定了分级施肥对这些参数的影响以及土壤肥力梯度的形成。
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引用次数: 0
Mitochondrial genetic homogeneity of South American leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) in Tamil Nadu, India 印度泰米尔纳德邦南美叶螨,Tuta absoluta (Meyrick)的线粒体遗传同质性(鳞翅目:蠓科)
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4502
K. Murugasridevi, S. Jeyarani, S. Mohan Kumar
The South American leafminer, Tuta absoluta is an exotic devastative pest on solanaceous vegetables, including tomatoes, which leads to a cent per cent economic loss in India. The molecular markers assist in assessing gene flow, migratory frequencies, and genetic variety, as well as helping to evaluate the genetic makeup and diversification of an exotic species population to indigenous ones. With this, the present study aimed to investigate the genetic divergence of T. absoluta in different districts of Tamil Nadu, India. The study depicted the examination of genetic divergence of T. absoluta by aiding amplified region of mitochondrial DNA encoding cytochrome oxidase I (COI) from the T. absoluta samples gathered from Coimbatore, Dharmapuri and Dindigul districts of Tamil Nadu. The findings showed that the phylogenetic tree constructed from all sequences of T. absoluta acquired from the NCBI (National Center for Biotechnology Information) and BOLD (The Barcode of Life Data System) databases exhibited 99 percent identity and aggregated together into a single clade. . 5Hence, the present study revealed the great genetic uniformity in T. absoluta populations in India and corroborates that most of the globe rely on the partial COI gene, evidenced by minimal nucleotide diversity.
南美叶螨是一种对茄类蔬菜(包括西红柿)具有破坏性的外来害虫,在印度造成了百分之百的经济损失。分子标记有助于评估基因流动、迁移频率和遗传多样性,并有助于评估外来物种种群与本地物种种群的遗传组成和多样性。因此,本研究旨在调查印度泰米尔纳德邦不同地区的绝对T.遗传差异。本研究通过对采集自泰米尔纳德邦哥印拜陀、达尔马布里和丁迪古尔地区的绝对T.绝对T.样本中编码细胞色素氧化酶I (COI)的线粒体DNA扩增区进行分析,描述了绝对T.绝对T.的遗传分化。研究结果表明,从NCBI (National Center for Biotechnology Information)和BOLD (The Barcode of Life Data System)数据库中获得的T. absoluta所有序列构建的系统发育树显示出99%的同一性,并聚集在一起形成一个单一的进化支。因此,本研究揭示了印度绝对T.种群的巨大遗传一致性,并证实了全球大部分地区依赖于部分COI基因,这是由最小核苷酸多样性证明的。
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引用次数: 0
Improving chemical, rheological and sensory properties of commercial low-fat cream by concentrate addition of whey proteins 通过浓缩添加乳清蛋白改善商品低脂奶油的化学、流变学和感官特性
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4672
Ali R. Mulakhudair, Dhia I. J. Al-Bedrani, Jasim M. S. Al-Saadi, Dhia Hattif Kadhim, Ali M. Saadi
The present study was conducted to determine the effect of whey protein concentrate (WPC) addition on the commercial low-fat cream's chemical, rheological and sensory properties. WPC was added to the low-fat cream (10% fat) in ratios of 1.0, 2.5, and 5.0% to represent the treatments C1, C2, and C3, respectively.In addition, a fat-rich, positive control treatment (C +) with a fat percentage of 30% and a negative low-fat control treatment (C-) with a fat percentage of 10% were investigated without adding WPC. Chemical tests were carried out, including the percentage of moisture, protein, fat, carbohydrates, total acidity, and pH, as well as rheological tests that included; hardness, springiness, and Cohesiveness. Also, a sensory evaluation was conducted. The results showed a decrease in the moisture percentage of the added treatments with the increment of the added WPC quantity. Also, a decrease in the fat percentage and pH of the WPC addition treatments was observed, combined with an increment in the percentage of protein, carbohydrates, ash, and total acidity. Regarding the microbiological properties, no bacterial or mycological contamination was observed during the manufacturing and storage periods. The results also showed the improvement of rheological and sensory characteristics by increasing the percentage of WPC addition compared with the positive and negative control treatments. The present study would be helpful in the production of low-fat cream fortified with whey proteins with high nutritional value.
本研究旨在确定乳清浓缩蛋白(WPC)的添加对商品低脂奶油的化学、流变学和感官特性的影响。将WPC以1.0、2.5和5.0%的比例添加到低脂奶油(10%脂肪)中,分别代表C1、C2和C3处理。此外,在不添加WPC的情况下,研究了脂肪率为30%的富脂阳性对照处理(C +)和脂肪率为10%的低脂阴性对照处理(C-)。进行了化学测试,包括水分、蛋白质、脂肪、碳水化合物、总酸度和pH值的百分比,以及流变学测试,包括;硬度、弹性和凝聚力。同时进行了感官评价。结果表明,随着木塑添加量的增加,各处理的含水率均呈下降趋势。此外,添加木聚糖处理降低了脂肪率和pH值,增加了蛋白质、碳水化合物、灰分和总酸度的百分比。关于微生物特性,在生产和储存期间未观察到细菌或真菌学污染。结果还表明,与正负对照相比,增加木塑添加量可改善其流变学和感官特性。本研究将有助于生产具有高营养价值的乳清蛋白强化低脂奶油。
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引用次数: 0
Estimation of Sensor-based site specific variable rate fertilizer application for maize (Zea mays L.) crop 基于传感器的玉米(Zea mays L.)作物定点变量施肥估算
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4724
Rita Patle, R. Kavitha, A. Surendrakumar, K. Balaji, S. Maragatham, B. Suthakar
Optical spectrometry sensors in crops offer a remarkable technological breakthrough in the field of variable-rate nitrogen fertilization. A field study was conducted during rainy (kharif) season of 2021 at the research farm of the Agricultural Engineering College and Research Institute Tamil Nadu Agricultural University Coimbatore to estimate maize crop nitrogen (N), Normalized Difference Vegetation Index (NDVI) value and chlorophyll content in hybrid maize COH (M) 8. Fertilizers were administered to the plots following the recommendations (250:75:75 kg NPK ha-1) given under Soil Test Crop Response, with a goal yield of 9t ha-1 predicted based on the initial soil available N, P, and K values. The experimental findings revealed a significant impact of nitrogen rate (P<0.001) on the percentage of nitrogen content in the leaves (% N leaf content). Additionally, there was a decrease in maize leaf chlorophyll content index over time, with ranges of 32.96 to 50.57, 28.78 to 41.78, 24.81 to 35.86, 22.12 to 28.54, and 14.34 to 20.56. On the contrary, the NDVI experienced an increase throughout the season, with ranges of 0.32 to 0.49, 0.30 to 0.55, 0.28 to 0.66, 0.46 to 0.88, and 0.56 to 0.84. The study will help foster sustainability within modern intensive farming practices by emphasizing the importance of reducing environmental pollution caused by applying Sensor-based site-specific nitrogen fertilizer for maize crop.
作物光学光谱传感器在变氮施肥领域提供了显著的技术突破。在2021年雨季(kharif),在泰米尔纳德邦农业大学哥印拜罗分校农业工程学院和研究所的研究农场进行了实地研究,估算了杂交玉米COH (M) 8作物氮素(N)、归一化植被指数(NDVI)值和叶绿素含量。按照土壤试验作物反应中给出的建议(250:75:75 kg NPK hm -1)施肥,根据初始土壤有效氮、磷和钾值预测目标产量为9t hm -1。结果表明,施氮量对叶片中氮含量百分比(% N叶含量)有显著影响(P<0.001)。玉米叶片叶绿素含量指数随时间呈下降趋势,分别为32.96 ~ 50.57、28.78 ~ 41.78、24.81 ~ 35.86、22.12 ~ 28.54和14.34 ~ 20.56。NDVI呈增加趋势,分别为0.32 ~ 0.49、0.30 ~ 0.55、0.28 ~ 0.66、0.46 ~ 0.88和0.56 ~ 0.84。该研究将通过强调减少因对玉米作物施用基于传感器的特定地点氮肥而造成的环境污染的重要性,有助于促进现代集约化农业实践的可持续性。
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引用次数: 0
Inter-annual variation of the mean temperature and rainfall in Chad and the solar activity during the period from 1950 to 2020 1950 - 2020年乍得平均气温、降雨量与太阳活动的年际变化
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4836
Ali Mahamat Nour, Masrabé Djimtolgar, Galmai Orozi
Given the increased variability of rainfall, the resurgence of extreme meteorological phenomena such as droughts, floods, heat waves, and violent winds and considering the influence of solar activity on certain meteorological parameters, a study on the variation of temperature and rainfall in relation to solar activity is necessary. The present study aimed to analyze the inter-annual evolution of meteorological parameters (mean temperature and rainfall) in Chad over the period from 1950 to 2020 in two geographically distinct areas (Sudanian and Sahelian zone) and then to establish a link between these parameters and solar activity to predict the variations of these two parameters over time. The rainfall results showed that the wettest years were 1959 in N'Djamena and 1951 in Moundou. On the other hand, the driest years recorded were 1984 in N'Djamena and 1998 in Moundou. The study of the mean temperature in these two areas during the same period led to the conclusion that the hottest year was 2010 in N'Djamena; however, in Moundou the hottest year recorded was 1997. Globally, in the two zones concerned, analysis of the results on variations in rainfall showed a downward trend. However, the study of average temperature showed a steady rise over the same period. The superposition of rainfall and mean temperature with the sunspot numbers Rz led to the conclusion that high sunspot activity increases temperature and thus warming, while low sunspot activity brings high precipitation.
鉴于降雨变异性增加,干旱、洪水、热浪和强风等极端气象现象死灰复燃,并考虑到太阳活动对某些气象参数的影响,有必要研究温度和降雨与太阳活动的关系。本研究旨在分析1950 - 2020年乍得两个地理区域(苏丹区和萨赫勒区)气象参数(平均温度和降雨量)的年际演变,然后建立这些参数与太阳活动之间的联系,以预测这两个参数随时间的变化。降水结果表明,恩贾梅纳和蒙杜的降水年份分别为1959年和1951年。另一方面,有记录以来最干旱的年份是1984年的恩贾梅纳和1998年的蒙杜。通过对这两个地区同期平均气温的研究,得出了2010年恩贾梅纳最热的结论;然而,在蒙杜,有记录以来最热的年份是1997年。就全球而言,在两个有关地区,对降雨变化结果的分析显示出下降趋势。然而,对平均气温的研究显示,同期气温稳步上升。降雨量和平均气温与太阳黑子数Rz的叠加可以得出太阳黑子活动高导致气温升高,而太阳黑子活动低导致降水增多的结论。
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引用次数: 0
Nanoparticles Titanium Dioxide with Thymus vulgaris extract in preservation and prolong the shelf life of cheese 纳米二氧化钛与寻常胸腺提取物在保存和延长奶酪的保质期
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4670
Hanan Abdullah Ali, Asmaa mansour Al-Hakeem, Quraish Abbas Kadhum
Cheese is considered a perishable food that is affected by microorganisms, and due to the properties of nanomaterials that have antimicrobial activity, they have been used synergistically with plant extracts in inhibiting the action of microorganisms that cause cheese spoilage. In this study, TiO2 (Titanium dioxide) nanoparticles were synthesized using Thymus vulgaris leaves extract (TVLE) . Atomic Force Microscopywas used to investigate Titanium dioxide/ TVLE nanoparticles characterize, which improved the regular spherical shape and granular distribution of nanoparticles with a particle size of 13 nm . The results showed that the minimum inhibitory concentration (MIC) of Titanium dioxide was at a concentration of 4 mg/ml and 80 mg/ml for TVLE, while it was 2 + 20 mg /ml for Titanium dioxide and TVLE .The inhibitory effect increased against Brucella melitensis recorded 12 mm when mixing Titanium dioxide and TVLE, compared with the inhibitory effect of Titanium dioxide, which recorded 10.5 mm and TVLE, with an inhibition diameter 8.1 mm. The effect of using titanium particles and thyme leaves’ extract was studied alone at a concentration of 4 mg/ml and 80 mg/ml and also when mixed in the microbial properties and pH of white soft cheese samples, which were prepared in the laboratory and contaminated with Brucella melitensis at refrigerated storage conditions (5Cº) for 21days. The effect of the synergism relationship between TiO2 / TVLE significantly reduced the total number of microorganisms in samples contaminated and uncontaminated with B. melitensis. Adding titanium dioxide and TVLE at concentrations of 4 and 80 mg/ml contributed significantly to maintaining the pH level during the storage period compared with the control group.
奶酪被认为是受微生物影响的易腐食品,由于纳米材料具有抗菌活性,因此它们与植物提取物协同使用,以抑制导致奶酪变质的微生物的作用。本研究以麝香叶提取物(TVLE)为原料合成了二氧化钛纳米颗粒。采用原子力显微镜对二氧化钛/ TVLE纳米颗粒进行表征,改善了纳米颗粒的规则球形和颗粒状分布,粒径为13 nm。结果表明,二氧化钛对tle的最小抑制浓度(MIC)分别为4 mg/ml和80 mg/ml,而二氧化钛和tle的最小抑制浓度(MIC)为2 + 20 mg/ml。二氧化钛和tle混合时对梅氏布鲁氏菌的抑制效果为12 mm,而二氧化钛和tle的抑制效果为10.5 mm,抑制直径为8.1 mm。研究了钛颗粒和百里香叶提取物分别在4 mg/ml和80 mg/ml的浓度下单独使用,以及混合使用对实验室制备的白色软质奶酪样品的微生物特性和pH值的影响,这些样品在冷藏条件下(5Cº)被布鲁氏菌污染了21天。TiO2 / TVLE之间的协同作用显著降低了被B. melitensis污染和未被B. melitensis污染的样品中微生物总数。与对照组相比,添加浓度为4和80 mg/ml的二氧化钛和tle对维持贮藏期间的pH水平有显著作用。
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引用次数: 0
Foliar application of Ascophyllum nodosum on improvement of photosynthesis, fruit setting percentage, yield and quality of tomato (Solanum lycopersicum L.) 叶面施药对番茄光合作用、坐果率、产量和品质的影响
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4725
Subramaniyan Lakshmi, Veerasamy Ravichandran, Selvaraj Anandakumar, Algarswamy Senthil, Loganathan Arul, Sengodan Radhamani, Ramasamy Anupriya
In recent days, liquid formulations of brown seaweed extract, Ascophyllum nodosum used as a biostimulant in agriculture. Various studies suggest that A. nodosum enhanced the growth and yield of agriculturally important crops, but still, there is a lack of information about the biostimulation effects on photosynthesis, flowering and fruit setting of tomato. Hence, the present study aimed to know the effect of foliar application of A. nodosum on photosynthesis, flowering, fruit setting, yield and quality of tomato. A biostimulant product, MC Set with A. nodosum extract applied to tomato as a foliar spray at rates of three different concentrations such as 1.0 L ha−1 (MS 1), 2.0 L ha−1 (MS 2), 3.0 L ha−1 (MS 3) for six times during flowering of 2nd (30 Days after transplanting – DAT), 3rd (40 DAT) and 4th (50 DAT) cluster and fruit setting of 2nd (60 DAT), 3rd (70 DAT) and 4th (80 DAT) cluster respectively. The MC Set treatments enhanced the plant photosynthesis, flower number and fruit number per cluster, yield and quality traits of tomato. However, the middle concentration MS 2 showed highest photosynthetic rate, stomatal conductance, SPAD value, flower and fruit in 2nd, 3rd and 4th cluster. It also had better average fruit weight and yield per plant and hectare and enhanced the quality parameters such as total soluble solids, ascorbic acid content, lycopene and total sugars compared to control and other two concentrations of MS Set. Hence, using A. nodosum extract on tomato growth could be a better sustainable crop production method.
近年来,棕色海藻提取物的液体配方,在农业中被用作生物刺激素。各种研究表明,结瘤草对重要农业作物的生长和产量有促进作用,但对番茄的光合作用、开花和坐果的生物刺激作用尚缺乏研究。因此,本研究旨在了解叶面施药对番茄光合作用、开花、坐果、产量和品质的影响。一种生物刺激素产品,MC套装,含刺藤提取物,在第二(移植后30天)、第三(40天)和第四(50天)花期和第二(60天)、第三(70天)和第四(80天)花期,分别以三种不同浓度的浓度,1.0 L ha - 1 (MS - 1)、2.0 L ha - 1 (MS - 2)、3.0 L ha - 1 (MS - 3)喷施于番茄叶面,喷施6次。MC组处理对番茄植株光合作用、每簇花数和果数、产量和品质性状均有促进作用。而中等浓度的MS 2在第2、3、4簇的光合速率、气孔导度、SPAD值、花和果均最高。与对照和其他两种浓度的MS Set相比,其单株和公顷单株平均单果重、单株单产和单株单产均有所提高,可溶性固形物总量、抗坏血酸含量、番茄红素和总糖等品质指标均有所提高。因此,在番茄生长过程中使用野刺藤提取物可能是一种较好的可持续作物生产方法。
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引用次数: 0
Optimising reaction variables for the preparation of superabsorbent iron fertiliser hydrogel using sugarcane bagasse: A sustainable approach to improve crop nutrient release 利用甘蔗渣制备高吸水性铁肥水凝胶的反应变量优化:一种改善作物养分释放的可持续方法
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4668
C. Bharaani Sri, R. Shanmugasundaram, S. Marimuthu, T. Chitdeshwari, A. Senthil, T. Kalaiselvi
Iron (Fe) is a vital micronutrient essential for crop growth and development. Utilisation of bio-based, environmentally friendly functional polymers is inevitable for society. As an alternative to the conventional Fe fertiliser, the present study aimed to synthesise a higher Fe percentage containing hydrogel with organic substances that can facilitate the slow release of nutrients, reduce fertiliser nutrient fixation, and minimise environmental pollution. The reaction variables were optimised for the preparation of superabsorbent using sugarcane bagasse and nano-zeolite-based slow-release Fe fertiliser (SR Fe) hydrogel. This was formulated by graft, co-polymerising acrylic acid, acrylamide, sugarcane bagasse, and nano-zeolite with N,N'-methylene bis-acrylamide as a crosslinker and ammonium persulfate as an initiator. Based on the swelling percentage, the reaction variables of the SR Fe fertiliser were standardised. The crosslinker (MBA - 10 wt%), the initiator (APS - 10 wt%), the filler (Nano-zeolite - 10 wt%), the monomer acrylamide composition (AAm - 2g), the acrylic acid content (AA - 7 ml), the reaction temperature (60oC), and the drying temperature (40oC) were chosen based on desirable swelling percentage and loaded with Fe fertiliser. The Fe fertiliser was loaded to sugarcane bagasse in different ratios (1:0.5, 1:1, 1:1.5, 1:2). The present study showed that the SR Fe fertiliser with the highest percentage of Fe (6.4%) in the ratio of sugarcane bagasse to Fe fertiliser of 1:2 could be used as an effective SR Fe fertiliser to supply nutrients slowly to crops to meet their nutrient needs and improve nutrient use efficiency.
铁(Fe)是作物生长发育所必需的重要微量元素。利用生物基、环保的功能聚合物是社会发展的必然趋势。作为传统铁肥料的替代品,本研究旨在用有机物质合成含铁率更高的水凝胶,从而促进养分的缓慢释放,减少肥料的养分固定,并将环境污染降到最低。优化了蔗渣与纳米沸石基缓释铁肥水凝胶制备高吸附剂的反应参数。以N,N′-亚甲基双丙烯酰胺为交联剂,过硫酸铵为引发剂,通过接枝、丙烯酸、丙烯酰胺、甘蔗渣和纳米沸石共聚合而成。根据溶胀率,对SR - Fe肥的反应变量进行了标准化。交联剂(MBA - 10 wt%)、引发剂(APS - 10 wt%)、填充剂(纳米沸石- 10 wt%)、单体丙烯酰胺组分(AAm - 2g)、丙烯酸含量(AA - 7 ml)、反应温度(60℃)、干燥温度(40℃)根据所需溶胀率进行选择,并加载铁肥。以不同比例(1:0.5、1:1、1:1.5、1:2)向甘蔗渣施用铁肥。本研究表明,蔗渣与铁肥比例为1:2时,含铁比例最高(6.4%)的SR铁肥可作为一种有效的SR铁肥,向作物缓慢供应养分,满足作物对养分的需求,提高养分利用效率。
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引用次数: 0
Effect of pigments of Pseudomonas aeruginosa on adhering and cytotoxicity of A549 cell line 铜绿假单胞菌色素对A549细胞株黏附及细胞毒性的影响
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4675
Sally Abdul Aziz Karim, Luma Abdulhady Zwain, Estabraq A. Mahmoud
Pseudomonas aeruginosa gram-negative, bacilli and facultative aerobic, P. aeruginosa cause cystic fibrosis patients, wounds, burns, and immunodeficienct patients, that have many virulence factors such as pyocyanin , cytotoxic ,biofilm formation and motility, Eighty-eight isolates belonging to P. aeruginosa were collected including the 66 clinical isolates obtained from different hospitals in Baghdad and were from different sources and 22 environmental isolates from previous studies of soil near oil fields. Microscopical and cultural characteristics were studied and diagnosed using biochemical tests, VITEC device, their ability to adhere to non-living (Polystyrene), living cell line (A549) and cytotoxicity of bacterial filtrate by MTT method. The results displayed that all isolates belonged to P. aeruginosa. The pigment-forming (pe26 – pc36) isolates and (PE33 – PC31) non-pigment-forming isolates were selected. That all selected bacteria were able to adhere to the Polystyrene and an epithelial carcinoma of lung (A549) was of more than 300 colony formation units in dilution (1:10) ,(1:1000), and (1:10000). The toxicity of the P. aeruginosa filtrate (pc36) isolated from clinical sources and producing pigments was 15.7, 34.5, 44 % at a concentration of 40, 60, 80 % respectively, while the isolate (pc31) that was isolated from clinical sources and non-producing pigment was 28.1, 75.2, 80.9 % at the same concentrations. As for the isolate (pe26),isolated from environmental sources and forming the pigment, the inhibition rate was 38.5, 83.1, 48.8 % at concentrations of 40, 60, 80) % respectively, and the isolate (PE33) that was isolated from environmental sources was 42, 73.4, 74.1 % at concentrations of 40, 60, 80 % respectively. The study will be helpful in evaluating the effect of pigment formation in P. aeruginosa on adhesion.
铜绿假单胞菌革兰氏阴性、杆菌和兼性需氧、铜绿假单胞菌引起囊性纤维化患者、伤口、烧伤和免疫缺陷患者,其毒力因子如pyocyanin、细胞毒性、生物膜形成和运动性等众多;共分离到铜绿假单胞菌88株,其中66株来自巴格达不同医院和不同来源的临床分离株,22株来自油田附近土壤的环境分离株。采用生化试验、VITEC装置、对非活(聚苯乙烯)、活细胞株(A549)的粘附能力和MTT法对细菌滤液的细胞毒性进行了研究和诊断。结果表明,所有分离株均属于铜绿假单胞菌。选择形成色素的分离株(pe26 - pc36)和不形成色素的分离株(PE33 - PC31)。所有选定的细菌都能够粘附在聚苯乙烯上,并且肺上皮癌(A549)在稀释(1:10),(1:1000)和(1:10000)时超过300个菌落形成单位。从临床来源和产生色素的P. aeruginosa滤液(pc36)在浓度为40%、60%和80%时的毒性分别为15.7%、34.5%和44%,而从临床来源和不产生色素的分离物(pc31)在相同浓度下的毒性分别为28.1%、75.2和80.9%。环境源分离物pe26在40、60、80浓度下的抑菌率分别为38.5%、83.1%、48.8%;环境源分离物PE33在40、60、80浓度下的抑菌率分别为42%、73.4%、74.1%。该研究有助于评价铜绿假单胞菌色素形成对粘附的影响。
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引用次数: 0
Spatial and seasonal fluctuations of Ichthyoplankton assemblage in the Mediterranean coast of Morocco (Southwestern Alboran Sea) 摩洛哥地中海沿岸(西南Alboran海)浮游鱼群落的空间和季节波动
Q4 Immunology and Microbiology Pub Date : 2023-09-19 DOI: 10.31018/jans.v15i3.4508
Lamia Diouri, Tarik Baibai, Najwa Hassou, Hinde Abdelouahab, Omar Ettahiri, Ahmed Makaoui, Abdelaziz Soukri, Mohamed Malki, Abdelilah Fahde
Ichthyoplankton represent the first life stages of fish. The study of ichthyoplankton is crucial to understanding marine ecosystems and plays an important role in the management and durability of fisheries resources. During March and October of 2019, two oceanographic ichthyoplankton surveys were conducted in the Mediterranean Sea of Morocco from Tanger to Saadia by studying the horizontal structure of the ichthyoplankton species assemblage and its relation to environmental parameters. The average surface water temperature was (15.8°C in spring and 16.4°C in autumn). The fish eggs and larvae were more abundant in March than in October (21268 eggs/10m² and 14084 larvae/10m² in spring and 10094 eggs/10m² and 13796 larvae/10m²). In both seasons, fish eggs from the families Sternoptychidae and Sparidae were dominant (10101 eggs/10m² and 7527 eggs/10m² in spring and 4422 eggs/10m² and 3928 eggs/10m² in fall, respectively). However, Myctophidae larvae were the most abundant in the study area, reaching 7601 larvae/10m² in spring and 11021 larvae/10m² in autumn. The environmental parameters: temperature, salinity and chlorophyll-a (surface) seem to directly influence the spatial distribution of ichtyoplancton. On the other hand, it seems that predation by jellyfish (Pelagia noctiluca)was a very important factor that added to the factors that influenced the distribution of the species of fish eggs and larvae. This work represents the first survey conducted in the southwestern Alboran Sea, which studies the horizontal structure of the ichthyoplankton species assemblage and its relation to environmental factors in the spring and autumn of 2019.
浮游鱼代表了鱼类生命的最初阶段。对浮游鱼的研究是了解海洋生态系统的关键,在渔业资源的管理和持久性方面起着重要作用。2019年3月和10月,在摩洛哥地中海丹吉尔至萨迪亚海域进行了两次海洋浮游鱼调查,研究了浮游鱼物种组合的水平结构及其与环境参数的关系。平均地表水温度为春季15.8°C,秋季16.4°C。3月鱼卵和幼鱼较10月丰富,春季为21268枚/10m²,14084枚/10m²,春季为10094枚/10m²,13796枚/10m²。两季均以胸鱼科和Sparidae科鱼卵为主,春季为10101个/10m²和7527个/10m²,秋季为4422个/10m²和3928个/10m²。但研究区蝇蛆科幼虫数量最多,春季为7601只/10m²,秋季为11021只/10m²。环境参数:温度、盐度和叶绿素a(表面)似乎直接影响鱼类植物的空间分布。另一方面,水母(Pelagia noctiluca)的捕食似乎是影响鱼卵和幼虫种类分布的一个非常重要的因素。本文首次在Alboran海西南部开展调查,研究了2019年春秋两季浮游鱼种类组合的水平结构及其与环境因子的关系。
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Journal of Applied and Natural Science
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