Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5309
N. Chauhan, M. Bakshi, S. K. Singh, J. B. Sharma, Article Info
Natural farming, a recent agricultural innovation emphasizing minimal inputs, helps boost crop production. While strawberries are conventionally cultivated, their natural farming potential remains untapped. In this regard, an experiment was conducted to evaluate the influence of Subhash Palekar natural farming (SPNF) and conventional farming (CF) systems on the vegetative growth and yield performances of five different strawberry cultivars viz. Capri, Winter Star, Winter Dawn, Camarosa, and Nabila under field trials in the Doaba region of Punjab conditions. The experiment included ten treatments and was laid out in the factorial randomized block design employing five distinct strawberry cultivars (Capri, Winter Star, Winter Dawn, Camarosa, and Nabila) as factor I, and farming techniques - SPNF and CF - as factor II. The results confirmed the supremacy of CF for vegetative growth and SPNF system for yield and yield attributing characters. Under the CF, cultivar Capri greatly outgrew other cultivars in terms of increased plant height (5.60 and 12.10 cm) and plant spread (14.87 and 23.63 cm EW; 13.00 and 24.59 cm NS) at 45 and 90 DAP, respectively. Contrarily, under the same farming method, the cultivar Camarosa displayed larger numbers of trifoliate leaves (4.80 and 14.50 at 45 and 90 DAP, respectively), chlorophyll index (56.90 SPAD), leaf area (45.84 cm2), and stem girth (2.45 cm). However, cultivar Capri produced the maximum fruits per plant (26.14) and yield per plant (328.53 g), while cultivar Camarosa resulted in the maximum average berry weight (16.53 g) and the greater yield efficiency (0.77 kg/cm2) under the SPNF technique.
{"title":"Comparative evaluation of strawberry cultivars under Subhash Palekar natural farming and conventional farming regimes in Doaba region of Punjab conditions","authors":"N. Chauhan, M. Bakshi, S. K. Singh, J. B. Sharma, Article Info","doi":"10.31018/jans.v16i1.5309","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5309","url":null,"abstract":"Natural farming, a recent agricultural innovation emphasizing minimal inputs, helps boost crop production. While strawberries are conventionally cultivated, their natural farming potential remains untapped. In this regard, an experiment was conducted to evaluate the influence of Subhash Palekar natural farming (SPNF) and conventional farming (CF) systems on the vegetative growth and yield performances of five different strawberry cultivars viz. Capri, Winter Star, Winter Dawn, Camarosa, and Nabila under field trials in the Doaba region of Punjab conditions. The experiment included ten treatments and was laid out in the factorial randomized block design employing five distinct strawberry cultivars (Capri, Winter Star, Winter Dawn, Camarosa, and Nabila) as factor I, and farming techniques - SPNF and CF - as factor II. The results confirmed the supremacy of CF for vegetative growth and SPNF system for yield and yield attributing characters. Under the CF, cultivar Capri greatly outgrew other cultivars in terms of increased plant height (5.60 and 12.10 cm) and plant spread (14.87 and 23.63 cm EW; 13.00 and 24.59 cm NS) at 45 and 90 DAP, respectively. Contrarily, under the same farming method, the cultivar Camarosa displayed larger numbers of trifoliate leaves (4.80 and 14.50 at 45 and 90 DAP, respectively), chlorophyll index (56.90 SPAD), leaf area (45.84 cm2), and stem girth (2.45 cm). However, cultivar Capri produced the maximum fruits per plant (26.14) and yield per plant (328.53 g), while cultivar Camarosa resulted in the maximum average berry weight (16.53 g) and the greater yield efficiency (0.77 kg/cm2) under the SPNF technique.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 24","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.4990
Muthana Salahuddin Ibrahim, Luma Abd Almunim Baker
The herb Hylocereus undatus, which is rich in phytochemicals and thought to contain antioxidants comparable to those in its peel, has a lot of promise for use in the food field. The presented study aimed to study the protective effect of the aqueous extrac and the active compounds (gallic acid) isolated from the dragon fruit (H. undates) to lessen the impact of rheumatoid arthritis (RA) induced by Freund's Complete Adjuvant (CFA)) by studying some of the changes in biochemical and histochemical parameters. The Wistar albino rats (male) were divided into four groups viz., Group I : A negative control group, dosed only with plain water orally, Group II : Induced for RA by CFA, a positive control group; Group III: dosed with aqueous extract of the fruit of H. undatus (kg/500 mg); and Group IV: dosed with gallic acid isolated from this fruit (kg/mg20). The results showed a significant increase in malondialdehyde (MDA), platelet, interleukin 6 ( IL-6), and RF in rats treated with CFA and a significant decrease in Hb compared to the negative control group. There was a significant decrease of MDA, Platelet, IL-6, and RF in the groups treated with aqueous extract and a significant increase in Hb compared with the positive control group while there was a significant decrease in each of MDA, platelets, IL-6, and RF. Based on a histological analysis, the study group's liver and kidneys had smaller lesions and different abnormalities than the control . Thus, the fruit plant's gallic acid extracts were protective in reducing RA damage caused by CFA and improving kidney and liver tissue.
火龙果（Hylocereus undatus）是一种草本植物，含有丰富的植物化学物质，其抗氧化剂含量与火龙果果皮中的抗氧化剂不相上下。本研究旨在研究从火龙果（H. undates）中分离出来的水性外c和活性化合物（没食子酸）对减轻弗氏完全佐剂（CFA）诱导的类风湿性关节炎（RA）的影响的保护作用，研究生化和组织化学参数的一些变化。将 Wistar 白化大鼠（雄性）分为四组，即：第一组：阴性对照组，仅口服白开水；第二组：CFA 诱导的 RA，阳性对照组；第三组：服用 H. undatus 果实的水提取物（kg/500 mg）；第四组：服用从该果实中分离出来的没食子酸（kg/mg20）。结果表明，与阴性对照组相比，用没食子酸处理的大鼠丙二醛（MDA）、血小板、白细胞介素 6（IL-6）和射频显著增加，血红蛋白显著下降。与阳性对照组相比，水提取物处理组的 MDA、血小板、白细胞介素 6 和射频显著降低，Hb 显著升高，而 MDA、血小板、白细胞介素 6 和射频均显著降低。根据组织学分析，研究组肝脏和肾脏的病变较小，异常情况也与对照组不同。因此，果实植物的没食子酸提取物在减少 CFA 引起的 RA 损伤和改善肝肾组织方面具有保护作用。
{"title":"Isolation and extraction of gallic acid from Hylocereus undatus and a biochemical and histological study on laboratory Wistar albino rats with induced rheumatoid arthritis","authors":"Muthana Salahuddin Ibrahim, Luma Abd Almunim Baker","doi":"10.31018/jans.v16i1.4990","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4990","url":null,"abstract":"The herb Hylocereus undatus, which is rich in phytochemicals and thought to contain antioxidants comparable to those in its peel, has a lot of promise for use in the food field. The presented study aimed to study the protective effect of the aqueous extrac and the active compounds (gallic acid) isolated from the dragon fruit (H. undates) to lessen the impact of rheumatoid arthritis (RA) induced by Freund's Complete Adjuvant (CFA)) by studying some of the changes in biochemical and histochemical parameters. The Wistar albino rats (male) were divided into four groups viz., Group I : A negative control group, dosed only with plain water orally, Group II : Induced for RA by CFA, a positive control group; Group III: dosed with aqueous extract of the fruit of H. undatus (kg/500 mg); and Group IV: dosed with gallic acid isolated from this fruit (kg/mg20). The results showed a significant increase in malondialdehyde (MDA), platelet, interleukin 6 ( IL-6), and RF in rats treated with CFA and a significant decrease in Hb compared to the negative control group. There was a significant decrease of MDA, Platelet, IL-6, and RF in the groups treated with aqueous extract and a significant increase in Hb compared with the positive control group while there was a significant decrease in each of MDA, platelets, IL-6, and RF. Based on a histological analysis, the study group's liver and kidneys had smaller lesions and different abnormalities than the control . Thus, the fruit plant's gallic acid extracts were protective in reducing RA damage caused by CFA and improving kidney and liver tissue.","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5179
N. Yadav, M. Sawariya, Ajay Kumar, H. Mehra, Jyoti Sharma, Sunil Kumar, S. Devi, V. Kaur, S. Arya
Linseed (Linum usitatissimum) is a versatile crop cultivated for its seeds, which are valuable source of ω-3 fatty acids. It adversely affected by soil salinity, as high salt levels can hinder their growth and reduce yields. To assess the potential for mitigating the adverse effects of high salinity concentrations, enhancing the resilience of three genotypes (Shekhar, Sheela, and Kartika) of linseed plants, this research aimed to find out the impact of Gibberellic acid (GA3) and Calcium (Ca) on various aspects of root morphology, osmotic potential of linseed, under varying levels of Cl- dominated salinity. The study employed three salinity levels (0, 5, and 10 dSm-1) and exogenous application of 10−6 M GA3 and/or 10 mg CaCl2 kg-1 in potted plants.The findings indicated that increasing salinity stress significantly (p≤0.05) affected root parameters, including total surface area(43.45%), average diameter(42.06%), total projected area(44.45%), length per volume (66.23%), root length, total root volume (73.23%), tips, forks,fine roots, and osmotic potential(66.67%). Correlations among linseed genotypes were observed between various root morphology and osmotic potential parameters. The application of GA3 and Ca effectively ameliorated the impact of salinity stress at its highest level (10 dSm-1), resulting in increased root parameters while decreasing the osmotic potential (Ψs). Both GA3 and Ca treatments significantly influenced root architecture and maintained optimal osmotic potential. The chloride-dominated salinity exerted inhibitory effects on all three genotypes’ (Shekhar, Sheela, and Kartika) root growth parameters while applying GA3 and Ca successfully mitigated these effects, enhancing root growth.
亚麻籽（Linum usitatissimum）是一种用途广泛的作物，其种子是ω-3 脂肪酸的宝贵来源。它受到土壤盐分的不利影响，因为高盐分会阻碍其生长并降低产量。为了评估减轻高浓度盐分不利影响的潜力，提高亚麻籽植物三种基因型（Shekhar、Sheela 和 Kartika）的抗逆性，本研究旨在找出赤霉素（GA3）和钙（Ca）在不同的 Cl- 主导盐分水平下对亚麻籽根系形态、渗透势等各方面的影响。研究采用了三种盐度（0、5 和 10 dSm-1），并在盆栽植物中外源施用 10-6 M GA3 和/或 10 mg CaCl2 kg-1。结果表明，盐分胁迫对亚麻子根系参数的影响非常明显（p≤0.05），包括总表面积（43.45%）、平均直径（42.06%）、总投影面积（44.45%）、单位体积长度（66.23%）、根长、根总量（73.23%）、根尖、根叉、细根和渗透势（66.67%）。不同亚麻籽基因型的根系形态和渗透势参数之间存在相关性。施用 GA3 和 Ca 能有效改善最高盐度（10 dSm-1）下盐胁迫的影响，在降低渗透势（Ψs）的同时增加了根系参数。GA3 和 Ca 处理都对根系结构产生了显著影响，并保持了最佳渗透压。以氯化物为主的盐度对所有三种基因型（Shekhar、Sheela 和 Kartika）的根系生长参数都产生了抑制作用，而 GA3 和 Ca 的应用则成功地减轻了这些影响，促进了根系生长。
{"title":"Influence of GA3 (gibberellic acid) and Ca(calcium) on root trait variation and osmotic potential of linseed (Linum usitatissimum L.) under chloride-dominated salinity","authors":"N. Yadav, M. Sawariya, Ajay Kumar, H. Mehra, Jyoti Sharma, Sunil Kumar, S. Devi, V. Kaur, S. Arya","doi":"10.31018/jans.v16i1.5179","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5179","url":null,"abstract":"Linseed (Linum usitatissimum) is a versatile crop cultivated for its seeds, which are valuable source of ω-3 fatty acids. It adversely affected by soil salinity, as high salt levels can hinder their growth and reduce yields. To assess the potential for mitigating the adverse effects of high salinity concentrations, enhancing the resilience of three genotypes (Shekhar, Sheela, and Kartika) of linseed plants, this research aimed to find out the impact of Gibberellic acid (GA3) and Calcium (Ca) on various aspects of root morphology, osmotic potential of linseed, under varying levels of Cl- dominated salinity. The study employed three salinity levels (0, 5, and 10 dSm-1) and exogenous application of 10−6 M GA3 and/or 10 mg CaCl2 kg-1 in potted plants.The findings indicated that increasing salinity stress significantly (p≤0.05) affected root parameters, including total surface area(43.45%), average diameter(42.06%), total projected area(44.45%), length per volume (66.23%), root length, total root volume (73.23%), tips, forks,fine roots, and osmotic potential(66.67%). Correlations among linseed genotypes were observed between various root morphology and osmotic potential parameters. The application of GA3 and Ca effectively ameliorated the impact of salinity stress at its highest level (10 dSm-1), resulting in increased root parameters while decreasing the osmotic potential (Ψs). Both GA3 and Ca treatments significantly influenced root architecture and maintained optimal osmotic potential. The chloride-dominated salinity exerted inhibitory effects on all three genotypes’ (Shekhar, Sheela, and Kartika) root growth parameters while applying GA3 and Ca successfully mitigated these effects, enhancing root growth.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.4736
Shivani More, H. Sahare
Epipremnum aureum, commonly known as pothos, is a hardy indoor foliage ornamental crop. Mutation breeding in Golden pothos is needed to meet the demand for more valuable and highly sought-after cultivars. The present study aimed to exploit variability on various parameters viz. new leaf emergence, survival percentage, plant height, length and width of leaves. The experiment was set up using a Complete Randomized Design (CRD) with 8 treatments and 3 replications. Rooted cuttings of Golden pothos were treated with varying concentrations of Ethyl Methane Sulfonate (EMS) for 4 hours, and a control treatment was included. The results showed that all the treated cuttings with EMS delays the new leaf emergence period compared to the control. Compared to the control, the minimum number of new leaf emergences was found in EMS-treated plants. The maximum survival percentage (100%) was recorded in 0.1%, 0.5% and control treatment. The lowest plant height (15.3 cm ) was observed in treatment T4 (1.5 % EMS) treated plants. However, maximum plant height was observed in the control treatment at 150 days of interval. In comparison to the control, EMS decreased the length and width of leaves at higher dosages. The minimum leaf length and width, i.e. 2.40 cm and 1.20 cm, were recorded in plants treated with EMS at 2%, while maximum length and width were observed in control and low EMS-treated plants. The study showed that ethyl methane sulfonate affected plant development characteristics and showed some dwarfness of money plants. This appears to be the first report of EMS treatment on pothos.
{"title":"Effect of chemical mutagen (Ethyl methane sulfonate) on Money plant (Epipremnum aureum)","authors":"Shivani More, H. Sahare","doi":"10.31018/jans.v16i1.4736","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4736","url":null,"abstract":"Epipremnum aureum, commonly known as pothos, is a hardy indoor foliage ornamental crop. Mutation breeding in Golden pothos is needed to meet the demand for more valuable and highly sought-after cultivars. The present study aimed to exploit variability on various parameters viz. new leaf emergence, survival percentage, plant height, length and width of leaves. The experiment was set up using a Complete Randomized Design (CRD) with 8 treatments and 3 replications. Rooted cuttings of Golden pothos were treated with varying concentrations of Ethyl Methane Sulfonate (EMS) for 4 hours, and a control treatment was included. The results showed that all the treated cuttings with EMS delays the new leaf emergence period compared to the control. Compared to the control, the minimum number of new leaf emergences was found in EMS-treated plants. The maximum survival percentage (100%) was recorded in 0.1%, 0.5% and control treatment. The lowest plant height (15.3 cm ) was observed in treatment T4 (1.5 % EMS) treated plants. However, maximum plant height was observed in the control treatment at 150 days of interval. In comparison to the control, EMS decreased the length and width of leaves at higher dosages. The minimum leaf length and width, i.e. 2.40 cm and 1.20 cm, were recorded in plants treated with EMS at 2%, while maximum length and width were observed in control and low EMS-treated plants. The study showed that ethyl methane sulfonate affected plant development characteristics and showed some dwarfness of money plants. This appears to be the first report of EMS treatment on pothos.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 39","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5330
Divyanshi Shukla, B. N. Tewari, S. P. Trivedi, Shraddha Dwivedi, Vivek Kumar, Vidyanand Tiwari
Raw ingredients directly affect the quality of processed foods. Along with improvements in processing technology, processed food production has grown significantly. The percentage of bakery products in total food consumption is very high, and soft bakery foods despite having a short shelf life, are well-liked because of their deliciousness. Special attention is paid to muffins with various combinations of nutritive ingredients and organoleptic qualities. Recent research studies have extensively covered the use of various fruits, vegetables, and grain and their by products as raw materials and value-added incorporation in the muffin to produce a nutrient-rich and highly valuable muffin. The present study provides a detailed observation of three major categories of incorporated ingredients, including fruits, vegetables, and their processing by products, as well as some other grain-based ingredients used as raw materials that add value. The study compiles a brief for their use in baked goods while reviewing the literature on ingredients. The findings of the literature analysis show an increase in the nutritional content of bioactive substances like antioxidants in the muffins. It has been revealed that using ingredients derived from fruits and vegetables is safer and more effective in terms of nutrition. Muffins may have a higher quality and better nutritional profile when certain combinations of fruit- and vegetable-based ingredients are added to the main ingredient. It will be helpful to researchers, food manufacturers, and small and medium-scale bakery unit operators about various aspects of the raw materials and properties connected with new muffin formulations and the standardization of stabilized products.
{"title":"Quality and functional attributes of muffins with incorporation of fruit, vegetable, and grain substitutes: A review","authors":"Divyanshi Shukla, B. N. Tewari, S. P. Trivedi, Shraddha Dwivedi, Vivek Kumar, Vidyanand Tiwari","doi":"10.31018/jans.v16i1.5330","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5330","url":null,"abstract":"Raw ingredients directly affect the quality of processed foods. Along with improvements in processing technology, processed food production has grown significantly. The percentage of bakery products in total food consumption is very high, and soft bakery foods despite having a short shelf life, are well-liked because of their deliciousness. Special attention is paid to muffins with various combinations of nutritive ingredients and organoleptic qualities. Recent research studies have extensively covered the use of various fruits, vegetables, and grain and their by products as raw materials and value-added incorporation in the muffin to produce a nutrient-rich and highly valuable muffin. The present study provides a detailed observation of three major categories of incorporated ingredients, including fruits, vegetables, and their processing by products, as well as some other grain-based ingredients used as raw materials that add value. The study compiles a brief for their use in baked goods while reviewing the literature on ingredients. The findings of the literature analysis show an increase in the nutritional content of bioactive substances like antioxidants in the muffins. It has been revealed that using ingredients derived from fruits and vegetables is safer and more effective in terms of nutrition. Muffins may have a higher quality and better nutritional profile when certain combinations of fruit- and vegetable-based ingredients are added to the main ingredient. It will be helpful to researchers, food manufacturers, and small and medium-scale bakery unit operators about various aspects of the raw materials and properties connected with new muffin formulations and the standardization of stabilized products.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5307
Abdallah Zayani, Salah-Eddine Laasli, Rachid Lahlali, Fatima El Bouchtaoui, N. E. Alem
The growth and development of citrus trees and the quality of their fruits are significantly influenced by the essential role of mineral plant nutrition. This study aimed to improve the productivity of the citrus (Citrus clementina Hort. Ex Tanaka) orchards by optimizing mineral nutrition through well-monitored fertilization and fertigation aspects using lysimetry. The first phase consisted of studying the behavior of the nutrients in the soil solution at the high root concentration level, analysis of the nutritional status of five varieties of clementines (Sidi Aissa, Cadoux, Orogrande, Nules, and Nour), and the variation of leaf composition in major elements (Nitrogen, phosphorus, potassium, magnesium, and calcium) for the five varieties along phenological stages, through the exploitation of the results of analyses carried out for the management of mineral nutrition in 47 plots of citrus fruit in the Souss-Massa region with the use of lysimetry. The results obtained in this first part revealed an important variation of the nutrients in the soil solution (55.24%) in terms of water inputs, fertilizers, and edaphic conditions, as well as a large variation of foliar compositions (62.98%). The second phase, consisting of regular monitoring of the mineral nutrition dynamics targeting the “Nules” variety grafted on “Citrus macrophylla” affirmed the importance of the citrus fertilization approach for determining the availability, distribution, nutrient interactions in soil solution and plant response by regular leaf diagnostics. Thus, mobile lysimetry offered a powerful tool for achieving both productivity and sustainability in citrus fertilization programs.
{"title":"Contribution to the optimization of citrus (Citrus clementina Hort. Ex Tanaka) fruit fertilization using mobile lysimetry in orchards of the Souss-Massa region, Morocco","authors":"Abdallah Zayani, Salah-Eddine Laasli, Rachid Lahlali, Fatima El Bouchtaoui, N. E. Alem","doi":"10.31018/jans.v16i1.5307","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5307","url":null,"abstract":"The growth and development of citrus trees and the quality of their fruits are significantly influenced by the essential role of mineral plant nutrition. This study aimed to improve the productivity of the citrus (Citrus clementina Hort. Ex Tanaka) orchards by optimizing mineral nutrition through well-monitored fertilization and fertigation aspects using lysimetry. The first phase consisted of studying the behavior of the nutrients in the soil solution at the high root concentration level, analysis of the nutritional status of five varieties of clementines (Sidi Aissa, Cadoux, Orogrande, Nules, and Nour), and the variation of leaf composition in major elements (Nitrogen, phosphorus, potassium, magnesium, and calcium) for the five varieties along phenological stages, through the exploitation of the results of analyses carried out for the management of mineral nutrition in 47 plots of citrus fruit in the Souss-Massa region with the use of lysimetry. The results obtained in this first part revealed an important variation of the nutrients in the soil solution (55.24%) in terms of water inputs, fertilizers, and edaphic conditions, as well as a large variation of foliar compositions (62.98%). The second phase, consisting of regular monitoring of the mineral nutrition dynamics targeting the “Nules” variety grafted on “Citrus macrophylla” affirmed the importance of the citrus fertilization approach for determining the availability, distribution, nutrient interactions in soil solution and plant response by regular leaf diagnostics. Thus, mobile lysimetry offered a powerful tool for achieving both productivity and sustainability in citrus fertilization programs.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 25","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5223
S. Swathi, C. Ravikumar, M. Thiruppathi, P. Senthilvalavan, Article Info
Integrated nutrient management (INM) practices and suitable cropping systems are an effective and promising way for enhancing crop productivity. However, the impact of proper INM practices and cultivation methods in rice cropping systems in dry tracts remains unclear. The field study evaluated the suitable cropping system under different rice establishment methods with INM practices in a sequential cropping system( rice-black gram, rice-maize, and rice-groundnut). Treatments were designed as two rice establishment methods [Direct Seeded Rice (DSR) and Transplanted Rice (TPR)] and five INM practices [RDF-Recommended Dose of Fertilizer (120:40:40 kg NPK ha-1) alone, RDF + Poultry manure compost @ 5 t ha-1, RDF+ Coir pith compost @ 5 t ha-1, RDF+ Green manure @ 6.25 t ha-1 and RDF-Green leaf manure @ 6.25 t ha-1]. TPR with the INM practice of RDF + poultry manure compost @ 5 t ha-1 recorded maximum productive tillers m-2 (351), number of filled grains panicle-1 (130.2), grain yield (6709 kg ha-1) and straw yield (9015 kg ha-1) of rice. Rice fallow crops (black gram, maize and groundnut) recorded 10 – 24,10-18 and 10 - 21 % higher values of yield components and yield, respectively, in DSR with INM treatment of RDF + Green manure @ 6.25 t ha-1 . Thus, RDF plus green manure or poultry manure application is an apt INM practice for a rice-based sequential cropping system under DSR, and the rice-black gram is an economical and sustainable cropping system for dry tracts of the southern zone of Tamil Nadu.
{"title":"Yield assessment of rice-black gram, rice-maize and rice-groundnut sequential cropping system influenced by rice establishment methods and nutrient management practices in the dry tract of the Southern zone of Tamil Nadu, India","authors":"S. Swathi, C. Ravikumar, M. Thiruppathi, P. Senthilvalavan, Article Info","doi":"10.31018/jans.v16i1.5223","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5223","url":null,"abstract":"Integrated nutrient management (INM) practices and suitable cropping systems are an effective and promising way for enhancing crop productivity. However, the impact of proper INM practices and cultivation methods in rice cropping systems in dry tracts remains unclear. The field study evaluated the suitable cropping system under different rice establishment methods with INM practices in a sequential cropping system( rice-black gram, rice-maize, and rice-groundnut). Treatments were designed as two rice establishment methods [Direct Seeded Rice (DSR) and Transplanted Rice (TPR)] and five INM practices [RDF-Recommended Dose of Fertilizer (120:40:40 kg NPK ha-1) alone, RDF + Poultry manure compost @ 5 t ha-1, RDF+ Coir pith compost @ 5 t ha-1, RDF+ Green manure @ 6.25 t ha-1 and RDF-Green leaf manure @ 6.25 t ha-1]. TPR with the INM practice of RDF + poultry manure compost @ 5 t ha-1 recorded maximum productive tillers m-2 (351), number of filled grains panicle-1 (130.2), grain yield (6709 kg ha-1) and straw yield (9015 kg ha-1) of rice. Rice fallow crops (black gram, maize and groundnut) recorded 10 – 24,10-18 and 10 - 21 % higher values of yield components and yield, respectively, in DSR with INM treatment of RDF + Green manure @ 6.25 t ha-1 . Thus, RDF plus green manure or poultry manure application is an apt INM practice for a rice-based sequential cropping system under DSR, and the rice-black gram is an economical and sustainable cropping system for dry tracts of the southern zone of Tamil Nadu. \u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial based therapeutics for cancer have gained a significant interest in recent decades. The present study relies on the synthesis, analysis, and conjugation of Salmonella Leucine-rich Proteins (SlrP) with mesoporous silica nanoparticles (MSNN) to evaluate their potential anticancer activity. The SlrP proteins were effectively produced and isolated from Salmonella enterica using Tryptic Soy Broth (TSB), and the subsequent SDS-PAGE analysis verified the presence of a band at around 72 KDa. The MSN synthesis yielded particles with an average diameter of 68.05±0.87 nm and a pore diameter of 7.1 nm. In addition, we synthesized MSNMPA and then conjugated them with SlrP. Characterization studies confirmed the effective conjugation. The cytotoxicity evaluation conducted on HeLa cells revealed no substantial modification in cell viability upon treatment with MSN alone. Nevertheless, when MSNMPA/SlrP was done, it demonstrated significant cytotoxic properties, as evidenced by an IC50 value of 10 µg/mL. The results indicate that SlrP-conjugated MSN (MSNMPA/SlrP) could be utilized as promising nanocarriers for delivering anticancer proteins.
{"title":"Anti-cancer activity of crude Slrp protein conjugated mesoporous silica nanoparticles in HeLa Cell Lines: An in vitro approach","authors":"Shivaprasad Panjala, Satyanarayana Swamy Vyshnava, Swathi Banapuram, Vihari Vasikarla, Paramasivam Kameshpandian, Muralidhara Rao Dowlathabad, R. Anupalli","doi":"10.31018/jans.v16i1.5285","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5285","url":null,"abstract":"Microbial based therapeutics for cancer have gained a significant interest in recent decades. The present study relies on the synthesis, analysis, and conjugation of Salmonella Leucine-rich Proteins (SlrP) with mesoporous silica nanoparticles (MSNN) to evaluate their potential anticancer activity. The SlrP proteins were effectively produced and isolated from Salmonella enterica using Tryptic Soy Broth (TSB), and the subsequent SDS-PAGE analysis verified the presence of a band at around 72 KDa. The MSN synthesis yielded particles with an average diameter of 68.05±0.87 nm and a pore diameter of 7.1 nm. In addition, we synthesized MSNMPA and then conjugated them with SlrP. Characterization studies confirmed the effective conjugation. The cytotoxicity evaluation conducted on HeLa cells revealed no substantial modification in cell viability upon treatment with MSN alone. Nevertheless, when MSNMPA/SlrP was done, it demonstrated significant cytotoxic properties, as evidenced by an IC50 value of 10 µg/mL. The results indicate that SlrP-conjugated MSN (MSNMPA/SlrP) could be utilized as promising nanocarriers for delivering anticancer proteins.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5396
Sutero S. Macabudbud, Jr.
Waste Coconut Kernels (WCK) and Sweet Potato (SP) peels are considered household wastes for animal feeding. The present study aimed to develop waste coconut kernel and sweet potato peels creamy roll as innovative guides for the Extension program guide. This experimental study used three formulations varying amounts of WCK with 100g, 150g and 200g; SP peels with 150g, 200g, and 250g mixed with milk solution, sugar and unsalted spring roll wrappers. The study focused on investigating the sensory attributes of the creamy roll development across different formulations, assessing its general acceptability in terms of color, flavor, odor and texture, identifying the most preferred formulation (F1, F2, and F3) based on the panel of tasters, and examining if there were significant differences among the product attributes. Based on Data analysis involved calculating weighted means and conducting One-way analysis of variance (ANOVA), the results revealed that F3, consisting of 250g of SP, 200g of WCK, 250g of milk solution, and 180g of sugar, was the most preferred in terms of flavor, odor and texture, with overall means of 3.67, 4.08, and 3.85, respectively. However, F2 was preferred only in color. A significant difference was observed in flavor indicated by a p-value of .042, lower than the predetermined alpha level of .05. Post-hoc analysis indicated differing perceptions between the 35 experienced consumers and 15 food experts. The most preferred formulation had a sensory acceptability rating of “acceptable” with a mean of 3.92. Based on the findings, the 250g SP peels and 200g WCK were adopted as an Extension program guide.
{"title":"Enhancing sustainability and flavor of waste coconut (Cocos nucifera) kernel and sweet potato (Ipomoea batatas) peels Creamy roll: Extension program guide","authors":"Sutero S. Macabudbud, Jr.","doi":"10.31018/jans.v16i1.5396","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5396","url":null,"abstract":"Waste Coconut Kernels (WCK) and Sweet Potato (SP) peels are considered household wastes for animal feeding. The present study aimed to develop waste coconut kernel and sweet potato peels creamy roll as innovative guides for the Extension program guide. This experimental study used three formulations varying amounts of WCK with 100g, 150g and 200g; SP peels with 150g, 200g, and 250g mixed with milk solution, sugar and unsalted spring roll wrappers. The study focused on investigating the sensory attributes of the creamy roll development across different formulations, assessing its general acceptability in terms of color, flavor, odor and texture, identifying the most preferred formulation (F1, F2, and F3) based on the panel of tasters, and examining if there were significant differences among the product attributes. Based on Data analysis involved calculating weighted means and conducting One-way analysis of variance (ANOVA), the results revealed that F3, consisting of 250g of SP, 200g of WCK, 250g of milk solution, and 180g of sugar, was the most preferred in terms of flavor, odor and texture, with overall means of 3.67, 4.08, and 3.85, respectively. However, F2 was preferred only in color. A significant difference was observed in flavor indicated by a p-value of .042, lower than the predetermined alpha level of .05. Post-hoc analysis indicated differing perceptions between the 35 experienced consumers and 15 food experts. The most preferred formulation had a sensory acceptability rating of “acceptable” with a mean of 3.92. Based on the findings, the 250g SP peels and 200g WCK were adopted as an Extension program guide.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.4978
Dhuha Muhammed Sadik, Ibrahim Hadi Mohammed
Cladophora glomerata has numerous biologically active properties and is considered one of the most essential medicinal algae. The purpose of this research was to investigate the anticancer efficacy of Cladophora glomerata algae extract against human hepatocellular carcinoma (HepG2), human cervical carcinoma (HeLa), and normal mouse embryonic fibroblast (MEF) cell lines. The needed algae was found in the Hassan Al-Hamoud River , Baquba, Iraq. The chemical detection of some chemical components of the ethanolic extract of C. glomerata revealed that the extract contains a group of active compounds. The study showed significant variation (p<0.05) among inhibition percentages of cancer cell line HepG2, Hella, and MEF cell lines that were treated with different concentrations (15.1, 31.2, 62.5, 125, 250, 500, and 1000) μg/ml of C. glomerata extract. The outcomes showed that increased inhibition percentages of the above cell line were associated with increased concentrations. The inhibition percentage of HepG2, Hella, and MEF cell lines were 1.6 ± 30.88, 1.7 ± 14.10, and 1.2 ± 2.31 at concentration 15.1 (mg/ml), and was 3.8 ± 84.90, 3.6 ± 88.29, and 3.9 ± 23.2, respectively at concentration 1000 (mg/ml). The study concluded that the C. glomerata extract with different concentrations significantly inhibited cancer cell lines (HepG2 and Hela) and ME because they have antiproliferative and antioxidant activity against cancers. The extract's inhibitory impact begins at low doses and increases with increasing concentration. The study would be beneficial to use macroalgae as new and sustainable sources of bioactive compounds against cancer.
{"title":"Evaluation of anticancer effect of Cladophora glomerata algae extract","authors":"Dhuha Muhammed Sadik, Ibrahim Hadi Mohammed","doi":"10.31018/jans.v16i1.4978","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4978","url":null,"abstract":"Cladophora glomerata has numerous biologically active properties and is considered one of the most essential medicinal algae. The purpose of this research was to investigate the anticancer efficacy of Cladophora glomerata algae extract against human hepatocellular carcinoma (HepG2), human cervical carcinoma (HeLa), and normal mouse embryonic fibroblast (MEF) cell lines. The needed algae was found in the Hassan Al-Hamoud River , Baquba, Iraq. The chemical detection of some chemical components of the ethanolic extract of C. glomerata revealed that the extract contains a group of active compounds. The study showed significant variation (p<0.05) among inhibition percentages of cancer cell line HepG2, Hella, and MEF cell lines that were treated with different concentrations (15.1, 31.2, 62.5, 125, 250, 500, and 1000) μg/ml of C. glomerata extract. The outcomes showed that increased inhibition percentages of the above cell line were associated with increased concentrations. The inhibition percentage of HepG2, Hella, and MEF cell lines were 1.6 ± 30.88, 1.7 ± 14.10, and 1.2 ± 2.31 at concentration 15.1 (mg/ml), and was 3.8 ± 84.90, 3.6 ± 88.29, and 3.9 ± 23.2, respectively at concentration 1000 (mg/ml). The study concluded that the C. glomerata extract with different concentrations significantly inhibited cancer cell lines (HepG2 and Hela) and ME because they have antiproliferative and antioxidant activity against cancers. The extract's inhibitory impact begins at low doses and increases with increasing concentration. The study would be beneficial to use macroalgae as new and sustainable sources of bioactive compounds against cancer.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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