Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5280
Hala M.N. Al-saily, Walaa Salih Hassan, H. Chabuk
Spirulina platensis is blue-green algae received significant attention for its high nutritional value, it is a source of powerful antioxidants. The cytotoxicity of crude extract is not well recorded. The aim of current study to evaluate the cytotoxicity of S. platensis extracts on colon cancer (CaCo-2), hepatic cancer (HepG2) cell lines, normal fibroblast cells line (HdFn) and also antioxidant activity. The percent of 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity was determined for serial concentrations of extract ranging from 3.125 to 200 μg/ml. Cell lines were treated for 24 hours with different concentrations of extract ranging from 25 to 400 µg/ml. Cell viability testing using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay which determined how the extract affected caspase 9 activity. The results revealed that extract had moderate antioxidant activity, showing the DPPH scavenging activity reached 58% in a concentration of 200μg/ml, and IC50 was 95.84 μg/ml. The extract significantly decreased CaCo-2 cell viability with IC50 99.12 µg/ml, compared to HdFn viability with IC50 157.6 µg/ml. On CaCo-2 cells, the extract's cytotoxicity was more evident (P< 0.05) than HdFn cells. The extract had more significant (P<0.05) cytotoxicity on cancer cell lines and also significantly decreased the viability of HepG2 cells with IC50 167.4 µg/ml, than the viability of HdFn with IC50 214.9 μg/ml. The extract revealed significantly higher (P< 0.05) cytotoxicity against HepG2 cells than the normal HdFn cells. This study concluded that the extract exerted a dose-dependent anti-proliferation effect on CaCo-2 cells and HepG2 cells by comparing them with HdFn cells.
{"title":"Anti-oxidant and cytotoxic activity of Spirulina platensis ethanolic extract against Caco-2 and HepG2 cancer cell lines","authors":"Hala M.N. Al-saily, Walaa Salih Hassan, H. Chabuk","doi":"10.31018/jans.v16i1.5280","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5280","url":null,"abstract":"Spirulina platensis is blue-green algae received significant attention for its high nutritional value, it is a source of powerful antioxidants. The cytotoxicity of crude extract is not well recorded. The aim of current study to evaluate the cytotoxicity of S. platensis extracts on colon cancer (CaCo-2), hepatic cancer (HepG2) cell lines, normal fibroblast cells line (HdFn) and also antioxidant activity. The percent of 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity was determined for serial concentrations of extract ranging from 3.125 to 200 μg/ml. Cell lines were treated for 24 hours with different concentrations of extract ranging from 25 to 400 µg/ml. Cell viability testing using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay which determined how the extract affected caspase 9 activity. The results revealed that extract had moderate antioxidant activity, showing the DPPH scavenging activity reached 58% in a concentration of 200μg/ml, and IC50 was 95.84 μg/ml. The extract significantly decreased CaCo-2 cell viability with IC50 99.12 µg/ml, compared to HdFn viability with IC50 157.6 µg/ml. On CaCo-2 cells, the extract's cytotoxicity was more evident (P< 0.05) than HdFn cells. The extract had more significant (P<0.05) cytotoxicity on cancer cell lines and also significantly decreased the viability of HepG2 cells with IC50 167.4 µg/ml, than the viability of HdFn with IC50 214.9 μg/ml. The extract revealed significantly higher (P< 0.05) cytotoxicity against HepG2 cells than the normal HdFn cells. This study concluded that the extract exerted a dose-dependent anti-proliferation effect on CaCo-2 cells and HepG2 cells by comparing them with HdFn cells.","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5336
S. Suntoro, Ganjar Herdiansyah, S. Minardi, Sri Hartati, Cindy Wulan Sari, Fidia Damayanti, Oktione Ismi Ardhasista
Organic fertilization is very important to maintain sustainable land and environmental productivity. Organic fertilizer from Azolla has the advantage of increasing soil fertility by increasing nutrient availability. This study aimed to determine the effect of Azolla application on the availability and uptake of N, P, and K of Inpari 32 variety rice plants. This research used 9 treatments: (T0= control, T1= 90 kg/h NPK, T2= 45 kg/h NPK, T3= 10 t/h Manure, T4= 2 t/h Manure + 90 kg/h NPK, T5= 10 t/h Rice straw compost, T6= 2 t/h Rice straw compost + 90 kg/h NPK, T7= 10 t/h Azolla, T8= 2 t/h Azolla + 90 kg/h NPK) with as control and 3 replications, so there were 27 experimental plots. This research was located in the paddy field of Madiun Regency, East Java. The results showed that Azolla, manure, and straw compost at a dose of 10 t/ha increased soil C-organic, soil Cation Exchange Capacity (CEC), and soil pH, which was still in the neutral range. Azolla fertilizer increased the highest soil total N compared to cow manure and straw compost and increased the efficiency of NPK fertilizer use. Azolla (T7) increased soil available P (27.02 ppm) and exchangeable K (17.63 me/100g soil). Azolla had the highest available P effect. Azolla (T7) and manure (T4) were affected by increasing K-exchange. Azolla fertilizer, manure, and rice straw compost increased vegetative plant growth (plant height, number of tillers, shoot and root biomass). Among the three, Azolla fertilizer had the highest effect. Organic fertilization affected N, P, and K uptake, which was highest in Azola fertilizer (T7). For manure, varied NPK fertilizer (T4) just showed an increase in P uptake, and compost fertilizer varied NPK fertilizer (T6) showed a rise in K uptake. A positive relationship existed between the availability and uptake of nutrients N, P, and K.
{"title":"Use of Azolla in organic farming on availability and uptake of N, P, K of rice paddy (Oryza Sativa, L.)","authors":"S. Suntoro, Ganjar Herdiansyah, S. Minardi, Sri Hartati, Cindy Wulan Sari, Fidia Damayanti, Oktione Ismi Ardhasista","doi":"10.31018/jans.v16i1.5336","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5336","url":null,"abstract":"Organic fertilization is very important to maintain sustainable land and environmental productivity. Organic fertilizer from Azolla has the advantage of increasing soil fertility by increasing nutrient availability. This study aimed to determine the effect of Azolla application on the availability and uptake of N, P, and K of Inpari 32 variety rice plants. This research used 9 treatments: (T0= control, T1= 90 kg/h NPK, T2= 45 kg/h NPK, T3= 10 t/h Manure, T4= 2 t/h Manure + 90 kg/h NPK, T5= 10 t/h Rice straw compost, T6= 2 t/h Rice straw compost + 90 kg/h NPK, T7= 10 t/h Azolla, T8= 2 t/h Azolla + 90 kg/h NPK) with as control and 3 replications, so there were 27 experimental plots. This research was located in the paddy field of Madiun Regency, East Java. The results showed that Azolla, manure, and straw compost at a dose of 10 t/ha increased soil C-organic, soil Cation Exchange Capacity (CEC), and soil pH, which was still in the neutral range. Azolla fertilizer increased the highest soil total N compared to cow manure and straw compost and increased the efficiency of NPK fertilizer use. Azolla (T7) increased soil available P (27.02 ppm) and exchangeable K (17.63 me/100g soil). Azolla had the highest available P effect. Azolla (T7) and manure (T4) were affected by increasing K-exchange. Azolla fertilizer, manure, and rice straw compost increased vegetative plant growth (plant height, number of tillers, shoot and root biomass). Among the three, Azolla fertilizer had the highest effect. Organic fertilization affected N, P, and K uptake, which was highest in Azola fertilizer (T7). For manure, varied NPK fertilizer (T4) just showed an increase in P uptake, and compost fertilizer varied NPK fertilizer (T6) showed a rise in K uptake. A positive relationship existed between the availability and uptake of nutrients N, P, and K. \u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5406
Gusti Agung, Gede Bawa, Sri Rahayu, Santi, Wiwik Susanah, Olan Suryanadi, Gek Indyan, Article Info
Curvularia verruculosa fungal causes leaf spot disease in rice plants. The bark extract of Michelia champaca could inhibit the growth of the fungi. The present research aimed to know the active compound responsible for antifungal activity. Extraction was done using the Maceration method, antifungal activity was measured using the Diffusion well method, and identification of active compounds was carried out using Gas chromatography-mass spectroscopy (GC-MS). The methanol extract obtained had a yield of 18.7%. It showed strong activity against C. verruculosa with an inhibition zone until 30.01 mm. The fractionation results showed that n-hexane extract (HE) was the strongest inhibiting the growth of C. verruculosa (32.45 mm), followed by chloroform extract (CE) (29.20 mm), while n-butanol extract (BE) was not active. Separating active compounds from HE extract was made using Column chromatography (CC) method with silica gel as the stationary phase and the mixture of n-hexane-acetone (3:1) as the mobile phase. This separation resulted in 5 combined fractions; HE3 and HE5 extracts showed very strong activity against C. verruculosa, with a diameter of the inhibition zone of 26.73 and 33.46 mm, respectively; HE2 extract showed strong activity with a diameter of the inhibition zone of 15.21 mm, while HE1 and HE4 extracts did not show activity. Identification using GC-MS, especially the HE3 extract, revealed that the extract contained two compounds: tributyl acetyl citrate and terephthalic acid, dodecyl-2-ethylhexyl ester. The result indicated that the bark extract of M. champaca had the potential to be a botanical fungicide.
{"title":"Active compounds of Michelia champaca bark extract against Curvularia verruculosa fungi causing leaf spot disease in rice (Oryza sativa L.)","authors":"Gusti Agung, Gede Bawa, Sri Rahayu, Santi, Wiwik Susanah, Olan Suryanadi, Gek Indyan, Article Info","doi":"10.31018/jans.v16i1.5406","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5406","url":null,"abstract":"Curvularia verruculosa fungal causes leaf spot disease in rice plants. The bark extract of Michelia champaca could inhibit the growth of the fungi. The present research aimed to know the active compound responsible for antifungal activity. Extraction was done using the Maceration method, antifungal activity was measured using the Diffusion well method, and identification of active compounds was carried out using Gas chromatography-mass spectroscopy (GC-MS). The methanol extract obtained had a yield of 18.7%. It showed strong activity against C. verruculosa with an inhibition zone until 30.01 mm. The fractionation results showed that n-hexane extract (HE) was the strongest inhibiting the growth of C. verruculosa (32.45 mm), followed by chloroform extract (CE) (29.20 mm), while n-butanol extract (BE) was not active. Separating active compounds from HE extract was made using Column chromatography (CC) method with silica gel as the stationary phase and the mixture of n-hexane-acetone (3:1) as the mobile phase. This separation resulted in 5 combined fractions; HE3 and HE5 extracts showed very strong activity against C. verruculosa, with a diameter of the inhibition zone of 26.73 and 33.46 mm, respectively; HE2 extract showed strong activity with a diameter of the inhibition zone of 15.21 mm, while HE1 and HE4 extracts did not show activity. Identification using GC-MS, especially the HE3 extract, revealed that the extract contained two compounds: tributyl acetyl citrate and terephthalic acid, dodecyl-2-ethylhexyl ester. The result indicated that the bark extract of M. champaca had the potential to be a botanical fungicide.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.4716
A. Sulaiman, Assala Saad
Bacteroides fragilis is the most anaerobic bacteria that infect humans, particularly in the abdominal cavity. Its pathogenesis is linked to numerous virulence factors. Understanding these factors and exploring alternative options for the use of antibiotics in the treatment of this bacterium, molecular techniques offer several advantages over traditional culture techniques because they are easier and more specific. The present study aimed to use specific primers for the 16sRNA and LuxR genes to identify B. fragilis. Genetic identification of the B. fragilis isolates was performed using the 16SrRNA gene, and the obtained sequences were submitted to National Centre for Biotechnology Information (NCBI) with accession numbers (OQ448827, OQ448828). Each strain was assigned a unique strain name, AS. AWB94 and AS. AWB79. From the total of all samples, it was found that the growth of various types of bacteria constituted ( 76%), and the samples that did not have growth formed (24%). It was noted that Bacteroidetes constituted only two isolates (2.7%), and these two isolates possessed the gene for quorum sensing (luxR gene), while the results confirmed that they do not possess the sialidase (nanH) enzyme gene. Both isolates possessed the quorum sensing gene (LuxR) out of one hundred samples. This suggests that the isolates have a quorum-sensing mechanism responsible for cell-to-cell communication, multidrug resistance, and biofilm formation.
{"title":"Detection of Bacteroides fragilis LuxR gene, involved in quorum sensing, among colitis patients in Mosul, Iraq","authors":"A. Sulaiman, Assala Saad","doi":"10.31018/jans.v16i1.4716","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4716","url":null,"abstract":"Bacteroides fragilis is the most anaerobic bacteria that infect humans, particularly in the abdominal cavity. Its pathogenesis is linked to numerous virulence factors. Understanding these factors and exploring alternative options for the use of antibiotics in the treatment of this bacterium, molecular techniques offer several advantages over traditional culture techniques because they are easier and more specific. The present study aimed to use specific primers for the 16sRNA and LuxR genes to identify B. fragilis. Genetic identification of the B. fragilis isolates was performed using the 16SrRNA gene, and the obtained sequences were submitted to National Centre for Biotechnology Information (NCBI) with accession numbers (OQ448827, OQ448828). Each strain was assigned a unique strain name, AS. AWB94 and AS. AWB79. From the total of all samples, it was found that the growth of various types of bacteria constituted ( 76%), and the samples that did not have growth formed (24%). It was noted that Bacteroidetes constituted only two isolates (2.7%), and these two isolates possessed the gene for quorum sensing (luxR gene), while the results confirmed that they do not possess the sialidase (nanH) enzyme gene. Both isolates possessed the quorum sensing gene (LuxR) out of one hundred samples. This suggests that the isolates have a quorum-sensing mechanism responsible for cell-to-cell communication, multidrug resistance, and biofilm formation.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seed germination of Capparis spinosa L. is highly important for ecology, medicine, and economics. The present study aimed to determine the effects of six pretreatments and two temperature regimes, T1: 9/35.7°C (Laboratory) and T2: 1/43°C (Greenhouse), on Capparis spinosa L. seeds' germination rate and latency time. Different pretreatments were tested, including scarification (P1) and seed imbibition in water (P2 and P3) and gibberellic acid (GA3) (P4, P5 and P6).The results showed that the highest germination rate (68.33%) was observed in the laboratory for the control seeds (T1, P0), followed by (58,33%) for seeds soaked in water for 48 hours (T1 P3),(56,67%) for seeds soaked in 200 ppm ofGA3 (T1, P4),(53,33%)for seeds soaked in 400 ppm and 600 ppm of GA3 (T1, P5andT1, P6), (48,33%)for seeds soaked in water for 24 hours (T1 P2), and (51,43%) for the control seeds in greenhouse (T2, P0), whereas the lowest germination rate (12.86%) was recorded in the greenhouse temperature, which was detrimental to seed germination, for seeds soaked in 600 ppm of GA3 (T2, P6). Germination latency times were shorter after soaking the seeds in water for 24 hours. Finally, seed germination of C. spinosa is subjected to several factors that may influence the total percentage of germination and latency time.
{"title":"Effect of gibberellic acid (GA3) and temperature on seed germination of Capparis spinosa L.","authors":"Basma Chiboub, Abdesselam Maatougui, Kaoutar Aboukhalid, Said Otouya, Fatima Zarqi, Abderrahmane Nazih, Mourad Baghour","doi":"10.31018/jans.v16i1.5287","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5287","url":null,"abstract":"Seed germination of Capparis spinosa L. is highly important for ecology, medicine, and economics. The present study aimed to determine the effects of six pretreatments and two temperature regimes, T1: 9/35.7°C (Laboratory) and T2: 1/43°C (Greenhouse), on Capparis spinosa L. seeds' germination rate and latency time. Different pretreatments were tested, including scarification (P1) and seed imbibition in water (P2 and P3) and gibberellic acid (GA3) (P4, P5 and P6).The results showed that the highest germination rate (68.33%) was observed in the laboratory for the control seeds (T1, P0), followed by (58,33%) for seeds soaked in water for 48 hours (T1 P3),(56,67%) for seeds soaked in 200 ppm ofGA3 (T1, P4),(53,33%)for seeds soaked in 400 ppm and 600 ppm of GA3 (T1, P5andT1, P6), (48,33%)for seeds soaked in water for 24 hours (T1 P2), and (51,43%) for the control seeds in greenhouse (T2, P0), whereas the lowest germination rate (12.86%) was recorded in the greenhouse temperature, which was detrimental to seed germination, for seeds soaked in 600 ppm of GA3 (T2, P6). Germination latency times were shorter after soaking the seeds in water for 24 hours. Finally, seed germination of C. spinosa is subjected to several factors that may influence the total percentage of germination and latency time.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5334
K. Chauhan, S. Rani, Article Info
The chronic metabolic disease known as diabetes mellitus causes hyperglycemia in the body. Antioxidant and antidiabetic qualities are well-known benefits of Hibiscus rosa sinensis (HRS). In this work, diabetic Wistar albino rats were used to assess the antidiabetic properties of HRS flower extract. A total of 18 animals were taken and divided into three groups (n = 6) – (Group 1): Normal control group, (Group 2): Diabetic control group, (Group 3): Diabetic group treated with a 125 mg/kg dose of HRS flower extract. Group 2 animals showed a progressive decrease in body weight, while Group 1 animals showed a considerable gain in body weight. After overcoming the weight loss, Group 3 animals also showed an increase in body weight that was similar to Group 1. Group 2 animals had blood glucose levels higher than 400 mg/dL, but Group 1 animals had blood glucose levels below 200 mg/dL throughout the experiment. Group 3 animals first had glucose levels higher than 350 mg/dL and then lower than 200 mg/dL, comparable to Group 1 animals. Upon histological examination, the pancreatic islets of Group 2 animals showed vacuolation, necrosis, and degeneration. The animals in Group 3 displayed regenerated islets of Langerhans and enhanced pancreatic anatomy. The animals in Group 3 also returned to normal in terms of body weight and blood glucose levels, similar to those in Group 1. These findings show that Hibiscus rosa sinensis has potential as an alternative diabetic treatment; further research is needed to fully understand its modes of action and long-term effects.
{"title":"Evaluation of antidiabetic potential of Hibiscus rosa sinensis on streptozotocin-induced diabetes on Wistar albino rats","authors":"K. Chauhan, S. Rani, Article Info","doi":"10.31018/jans.v16i1.5334","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5334","url":null,"abstract":"The chronic metabolic disease known as diabetes mellitus causes hyperglycemia in the body. Antioxidant and antidiabetic qualities are well-known benefits of Hibiscus rosa sinensis (HRS). In this work, diabetic Wistar albino rats were used to assess the antidiabetic properties of HRS flower extract. A total of 18 animals were taken and divided into three groups (n = 6) – (Group 1): Normal control group, (Group 2): Diabetic control group, (Group 3): Diabetic group treated with a 125 mg/kg dose of HRS flower extract. Group 2 animals showed a progressive decrease in body weight, while Group 1 animals showed a considerable gain in body weight. After overcoming the weight loss, Group 3 animals also showed an increase in body weight that was similar to Group 1. Group 2 animals had blood glucose levels higher than 400 mg/dL, but Group 1 animals had blood glucose levels below 200 mg/dL throughout the experiment. Group 3 animals first had glucose levels higher than 350 mg/dL and then lower than 200 mg/dL, comparable to Group 1 animals. Upon histological examination, the pancreatic islets of Group 2 animals showed vacuolation, necrosis, and degeneration. The animals in Group 3 displayed regenerated islets of Langerhans and enhanced pancreatic anatomy. The animals in Group 3 also returned to normal in terms of body weight and blood glucose levels, similar to those in Group 1. These findings show that Hibiscus rosa sinensis has potential as an alternative diabetic treatment; further research is needed to fully understand its modes of action and long-term effects.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.4728
Noor S. Naji, Y. Jassim, Lilian Qasim Alwan Al-Budairi, Zainab Mohammed Abass
Soil bacteria have an effective role in dissolving soil potassium. Bacillus mucilaginosus plays an effective role in dissolving potassium in the soil so that the plant may absorb it easily. The present study aimed to test the efficiency of bacteria in dissolving potassium present in the soil surrounding the roots of crops. B. mucilaginosus was isolated and diagnosed from the rhizosphere soil of Celery, Wheat, Basil and Alfalfa plants. The diagnosis included studying the isolates' culture, microscopic and biochemical characteristics. The laboratory study also included testing the efficiency of these bacterial isolates in dissolving potassium compounds in Modified Aleksandrov agar medium and estimating the dissolution coefficient. The results of isolation and identification of bacteria isolated from 19 out of 50 soil samples planted with different crops (Celery, Wheat, Basil and Alfalfa) showed that 8 isolates could dissolve potassium. The results of the microscopic examination of these eight isolates showed that they were sticky in shape, positive for Gram-staining, forming spores and the capsule, while the movement examination showed that they were positive for these tests (movement test). The biochemical tests and cultural characteristics showed that the eight isolates bear the characteristics of B. mucilaginosus. The results showed that the dissolution coefficient of potassium for the different isolates ranged between 2.28 and 1.14, while the type of sugar added to the culture medium increased the efficiency of bacterial isolates for potassium solubility. The study demonstrated the bacteria's efficiency in the rhizosphere region in dissolving potassium, which helps the plant use it easily.
{"title":"Efficiency of Bacillus mucilaginosus isolated from the soil in dissolving potassium in its microenvironment","authors":"Noor S. Naji, Y. Jassim, Lilian Qasim Alwan Al-Budairi, Zainab Mohammed Abass","doi":"10.31018/jans.v16i1.4728","DOIUrl":"https://doi.org/10.31018/jans.v16i1.4728","url":null,"abstract":"Soil bacteria have an effective role in dissolving soil potassium. Bacillus mucilaginosus plays an effective role in dissolving potassium in the soil so that the plant may absorb it easily. The present study aimed to test the efficiency of bacteria in dissolving potassium present in the soil surrounding the roots of crops. B. mucilaginosus was isolated and diagnosed from the rhizosphere soil of Celery, Wheat, Basil and Alfalfa plants. The diagnosis included studying the isolates' culture, microscopic and biochemical characteristics. The laboratory study also included testing the efficiency of these bacterial isolates in dissolving potassium compounds in Modified Aleksandrov agar medium and estimating the dissolution coefficient. The results of isolation and identification of bacteria isolated from 19 out of 50 soil samples planted with different crops (Celery, Wheat, Basil and Alfalfa) showed that 8 isolates could dissolve potassium. The results of the microscopic examination of these eight isolates showed that they were sticky in shape, positive for Gram-staining, forming spores and the capsule, while the movement examination showed that they were positive for these tests (movement test). The biochemical tests and cultural characteristics showed that the eight isolates bear the characteristics of B. mucilaginosus. The results showed that the dissolution coefficient of potassium for the different isolates ranged between 2.28 and 1.14, while the type of sugar added to the culture medium increased the efficiency of bacterial isolates for potassium solubility. The study demonstrated the bacteria's efficiency in the rhizosphere region in dissolving potassium, which helps the plant use it easily.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140389310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5405
R. S. Makar, Sahar A. Shahin, Mohamed Abd El-Hady
Wheat is considered one of the most essential crops for Egypt. Nevertheless, it is also one of its largest imports. Therefore, it is important to develop an accurate wheat suitability model to define the most suitable areas for its production. This study aimed to develop a parametric-based Analytical Hierarchy Process (AHP) using Geographic Information Systems (GIS) for land suitability evaluation of wheat in a selected area in El-Beheira governorate, Egypt. The climatic and land parameters influencing wheat production in the studied area were selected and rated according to the parametric method. These parameters included slope, texture, calcium carbonate, sum of basic cations, pH, organic matter, salinity, exchangeable sodium percentage and mean temp. of the growing cycle. The rated parameters were processed according to the AHP. The results were compared with Storie and the Square root methods and field observations. When validated using field observations, the developed method had a higher accuracy suitability evaluation for wheat cultivation in the studied area than the other two methods. According to the developed method, almost all of the studied areas could be classified as very suitable (S1) for wheat cultivation. On the other hand, the wheat suitability evaluation according to the other two methods indicated that most of the studied area could be classified as moderately suitable (S2) and marginally suitable (S3), which contradicted the field survey.
{"title":"Development of a parametric-based Analytical Hierarchy Process (AHP) utilizing Geographic Information Systems (GIS) for wheat land suitability evaluation","authors":"R. S. Makar, Sahar A. Shahin, Mohamed Abd El-Hady","doi":"10.31018/jans.v16i1.5405","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5405","url":null,"abstract":"Wheat is considered one of the most essential crops for Egypt. Nevertheless, it is also one of its largest imports. Therefore, it is important to develop an accurate wheat suitability model to define the most suitable areas for its production. This study aimed to develop a parametric-based Analytical Hierarchy Process (AHP) using Geographic Information Systems (GIS) for land suitability evaluation of wheat in a selected area in El-Beheira governorate, Egypt. The climatic and land parameters influencing wheat production in the studied area were selected and rated according to the parametric method. These parameters included slope, texture, calcium carbonate, sum of basic cations, pH, organic matter, salinity, exchangeable sodium percentage and mean temp. of the growing cycle. The rated parameters were processed according to the AHP. The results were compared with Storie and the Square root methods and field observations. When validated using field observations, the developed method had a higher accuracy suitability evaluation for wheat cultivation in the studied area than the other two methods. According to the developed method, almost all of the studied areas could be classified as very suitable (S1) for wheat cultivation. On the other hand, the wheat suitability evaluation according to the other two methods indicated that most of the studied area could be classified as moderately suitable (S2) and marginally suitable (S3), which contradicted the field survey.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bangladesh is extremely vulnerable to climate change, and vegetation indices serve as sensitive indicators. Due to the impacts of climate change, the cropping intensity of Southern region of Bangladesh is very low. So, this study aimed to analyze the changes in vegetation cover over time using the Normalized Difference Vegetation Index (NDVI) and identify the use of Climate Smart Agriculture (CSA) technologies and the benefits of using such technologies. A questionnaire survey was carried out by purposive random sampling method to detect 120 farmers’ socioeconomic status, hazards faced by climate change, adopted climate smart agricultural practices and its benefits for assessing Adaptive Strategy Index (ASI) in Amtali upazila of Barguna district and Kalapara upazila of Patuakhali district. NDVI analysis of multi-spectral remote sensing data from 2012 and 2022 indicated the extent of sparse vegetation of Kalapara has increased. Western part of Amtali upazila, fallow areas have become lessened in 2022 (354.55 km2) compared to 2012 (368.78 km2) due to adopting different CSA practices. Saline-tolerant crop varieties, sunflowers, and watermelon cultivation were the highest ranked among the CSA practices, with 301, 300, and 296 ASI, respectively. Calculated weighted average of CSA practices indicated the reduction of production cost, increased family income (49.19%) and cropping intensity (51.67%), which impacts developed social bonding.
{"title":"Detection of vegetation cover change in the Southern region of Bangladesh using the Normalized Difference Vegetation Index (NDVI) and Climate Smart Agriculture (CSA) practices","authors":"Nazmun Naher, Jayan Saosan Zannat, Jahamina Jarin Sharna","doi":"10.31018/jans.v16i1.5398","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5398","url":null,"abstract":"Bangladesh is extremely vulnerable to climate change, and vegetation indices serve as sensitive indicators. Due to the impacts of climate change, the cropping intensity of Southern region of Bangladesh is very low. So, this study aimed to analyze the changes in vegetation cover over time using the Normalized Difference Vegetation Index (NDVI) and identify the use of Climate Smart Agriculture (CSA) technologies and the benefits of using such technologies. A questionnaire survey was carried out by purposive random sampling method to detect 120 farmers’ socioeconomic status, hazards faced by climate change, adopted climate smart agricultural practices and its benefits for assessing Adaptive Strategy Index (ASI) in Amtali upazila of Barguna district and Kalapara upazila of Patuakhali district. NDVI analysis of multi-spectral remote sensing data from 2012 and 2022 indicated the extent of sparse vegetation of Kalapara has increased. Western part of Amtali upazila, fallow areas have become lessened in 2022 (354.55 km2) compared to 2012 (368.78 km2) due to adopting different CSA practices. Saline-tolerant crop varieties, sunflowers, and watermelon cultivation were the highest ranked among the CSA practices, with 301, 300, and 296 ASI, respectively. Calculated weighted average of CSA practices indicated the reduction of production cost, increased family income (49.19%) and cropping intensity (51.67%), which impacts developed social bonding.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-20DOI: 10.31018/jans.v16i1.5273
K. Bama, S. Elamathi, T. Sivasankari, A. Anuratha, Article Info
Cauvery Delta is the major rice-growing tract of Tamil Nadu. Continuous waterlogging is inevitable in the delta region due to unexpected heavy rain, leading to stunted crop growth and poor soil conditions. The present study aimed to alleviate the waterlogging-associated problems of stunted growth, crop nutrition deficiency, heavy algal growth and poor soil aeration issues in the heavy clayey soils of deltaic region of Tamil Nadu. Field experiments were laid out with the treatments viz., CuSO4,, (5kg/ha) (T1), Gypsum (500 kg/ha) (T2), Conoweeding+Alternate Wetting and Drying Irrigation(AWDI) (T3), microbial consortia (K and Zn solubilising Bacteria 500 ml/ha) (T4) control (T5) and combination of all (T6) except CuSO4 along with control . The results indicated that the T6 recorded higher plant height (110.2 cm), productive tillers/m2 of 332, filled grains of 118.3 and less chaffy grains of 20.3 nos, high nutrient status of 265 kg/ha of available N,35.4 kg/ha of available P,342 kg/ha of available K, 21.1meq/100 g of Ca,8.2 meq/100 g of Mg and root length and volume. The algal population in terms of dry biomass was reduced to 3.1 (g/m2) from the control group of 11.2 (g/m2) at 15 days after imposing treatment. The per cent increase of 26.0 % grain yield was also recorded in the combination treatment over control. From the present research, combined application of gypsum @500 kg/ha +Cono weeding twice +AWDI and microbial consortia of Zn and K solubilising bacteria @500ml/ha could be recommended for better soil environment and rice production in the delta region.
{"title":"Mitigating of waterlogging associated problems by the management practices in the rice ecosystem of the Deltaic zone of Tamil Nadu","authors":"K. Bama, S. Elamathi, T. Sivasankari, A. Anuratha, Article Info","doi":"10.31018/jans.v16i1.5273","DOIUrl":"https://doi.org/10.31018/jans.v16i1.5273","url":null,"abstract":"Cauvery Delta is the major rice-growing tract of Tamil Nadu. Continuous waterlogging is inevitable in the delta region due to unexpected heavy rain, leading to stunted crop growth and poor soil conditions. The present study aimed to alleviate the waterlogging-associated problems of stunted growth, crop nutrition deficiency, heavy algal growth and poor soil aeration issues in the heavy clayey soils of deltaic region of Tamil Nadu. Field experiments were laid out with the treatments viz., CuSO4,, (5kg/ha) (T1), Gypsum (500 kg/ha) (T2), Conoweeding+Alternate Wetting and Drying Irrigation(AWDI) (T3), microbial consortia (K and Zn solubilising Bacteria 500 ml/ha) (T4) control (T5) and combination of all (T6) except CuSO4 along with control . The results indicated that the T6 recorded higher plant height (110.2 cm), productive tillers/m2 of 332, filled grains of 118.3 and less chaffy grains of 20.3 nos, high nutrient status of 265 kg/ha of available N,35.4 kg/ha of available P,342 kg/ha of available K, 21.1meq/100 g of Ca,8.2 meq/100 g of Mg and root length and volume. The algal population in terms of dry biomass was reduced to 3.1 (g/m2) from the control group of 11.2 (g/m2) at 15 days after imposing treatment. The per cent increase of 26.0 % grain yield was also recorded in the combination treatment over control. From the present research, combined application of gypsum @500 kg/ha +Cono weeding twice +AWDI and microbial consortia of Zn and K solubilising bacteria @500ml/ha could be recommended for better soil environment and rice production in the delta region.\u0000 ","PeriodicalId":14996,"journal":{"name":"Journal of Applied and Natural Science","volume":" 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140388634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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