Pub Date : 2021-07-26DOI: 10.9734/jabb/2021/v24i630220
O. John, Christiana E. Asuquo, M. M. Akpan
The Automated Teller Machine (ATM) and Point of Sales (POS) machine user interface of different banks, schools, hospitals, restaurants/eateries, shopping malls and petrol/gas stations in Uyo metropolis were bacteriologically assessed in the dry and wet seasons using cultural technique. The result of fomites key pads indicate total heterotrophic bacteria counts (THBC) was in the order: Banks > Hospitals > Schools > Petrol/Gas stations > shopping malls > Restaurants/Eateries in both seasons. There was an increase in the bacterial counts of the fomites from all locations in the wet season compared to the dry season and the difference significant (p = 0.05). The THBC from ATMs in banks, hospitals and schools ranged from 5.6 ± 0.1 to 7.8 ± 0.6 Log₁₀CFU/cm2 and from POS 3.4 ± 0.3 to 5.0 ± 0.2 Log₁₀CFU/cm2) for Petrol/Gas stations, shopping malls and Restaurants/Eateries in the wet season. The dry season revealed THBC range of 4.1± 0.6 to 6.4 ± 0.3 Log₁₀CFU/cm2 for ATMs and 2.6 ± 0.8 to 3.8 ± 0.1 Log₁₀CFU/cm2 for POS. Organisms associated with the fomites were Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and species of Bacillus, Pseudomonas, Proteus, Streptococcus, Salmonella, Shigella, Micrococcus, Vibrio and Klebsiella. The highest and least frequency of occurrence in the dry season indicated Micrococcus (17.2%) and Vibrio (1.4%) in relation to Staphylococcus aureus (18.2%) and Proteus sp. (2.8%) in the wet season. The Gram-positive organisms exhibited high and low susceptibility to Gentamycin and Augmentin compared to Ofloxacin and Amoxicillin respectively in Gram-negative bacteria. The results indicate ATM and POS machine user interface as possible sources of pathogenic organisms. Therefore, regular cleaning of ATM and POS user interface and public awareness on the need for adequate personal hygienic practice after the use of these machines is recommended to reduce associated risks.
{"title":"Bacteriological Assessment of Automated Teller and Point of Sales Machine User Interface in Uyo Metropolis, Nigeria","authors":"O. John, Christiana E. Asuquo, M. M. Akpan","doi":"10.9734/jabb/2021/v24i630220","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i630220","url":null,"abstract":"The Automated Teller Machine (ATM) and Point of Sales (POS) machine user interface of different banks, schools, hospitals, restaurants/eateries, shopping malls and petrol/gas stations in Uyo metropolis were bacteriologically assessed in the dry and wet seasons using cultural technique. The result of fomites key pads indicate total heterotrophic bacteria counts (THBC) was in the order: Banks > Hospitals > Schools > Petrol/Gas stations > shopping malls > Restaurants/Eateries in both seasons. There was an increase in the bacterial counts of the fomites from all locations in the wet season compared to the dry season and the difference significant (p = 0.05). The THBC from ATMs in banks, hospitals and schools ranged from 5.6 ± 0.1 to 7.8 ± 0.6 Log₁₀CFU/cm2 and from POS 3.4 ± 0.3 to 5.0 ± 0.2 Log₁₀CFU/cm2) for Petrol/Gas stations, shopping malls and Restaurants/Eateries in the wet season. The dry season revealed THBC range of 4.1± 0.6 to 6.4 ± 0.3 Log₁₀CFU/cm2 for ATMs and 2.6 ± 0.8 to 3.8 ± 0.1 Log₁₀CFU/cm2 for POS. Organisms associated with the fomites were Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis and species of Bacillus, Pseudomonas, Proteus, Streptococcus, Salmonella, Shigella, Micrococcus, Vibrio and Klebsiella. The highest and least frequency of occurrence in the dry season indicated Micrococcus (17.2%) and Vibrio (1.4%) in relation to Staphylococcus aureus (18.2%) and Proteus sp. (2.8%) in the wet season. The Gram-positive organisms exhibited high and low susceptibility to Gentamycin and Augmentin compared to Ofloxacin and Amoxicillin respectively in Gram-negative bacteria. The results indicate ATM and POS machine user interface as possible sources of pathogenic organisms. Therefore, regular cleaning of ATM and POS user interface and public awareness on the need for adequate personal hygienic practice after the use of these machines is recommended to reduce associated risks.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73897762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-24DOI: 10.9734/JABB/2021/V24I630219
A. Mohammed, D. Mhya
Aims: Bambara nut seeds have been reported as a good source of food with high fiber and nutritional contents, and is consumed by persons with diabetes as one of the dietary therapy. However, the plant seeds are of different varieties and whether they might have vary biological effects on renal and liver functions is not fully known. This has prompted the study to evaluate effects of consuming different varieties of Bambara Nut seeds on liver and kidney of both diabetic and non-diabetic rats. Methodology: Four Bambara nut seeds varieties were dehulled and each heated at 60oC, cooled then grounded into flour using a mechanical grinder. The flours were used to formulate feeds which were given to different diabetic and non-diabetic rats’ groups as follows; group A (ALK01 feed), group B (ALK02 feed), group C (ALK03 feed), group D (ALK04 feed), group E (Basal feed), and group F (Normal animal’s feed). The animals were fed for 28 days then sacrifice, blood collected and serum separated and used for hepatic and renal parameters analysis. Results: The results of the study showed decrease levels of serum total protein and albumin, and elevation of AST and ALT activities as well as urea and creatinine concentrations in diabetic rats fed basal and normal diets, whereas, reverse in these parameters were recorded from diabetic rats fed varieties of Bambara nut seeds’ formulated feeds. Conclusion: Findings from the study suggest consumption of Bambara nut seeds of different varieties is safe and has ability to ameliorate diabetic hepatic and renal failure in a variety dependent manner where; ALK 04 is more likely the most effective.
{"title":"Effects of Consuming Different Varieties of Bambara Nut (Vigna subterranea) Seeds on Liver and Kidney of Diabetic and Non-diabetic Subject","authors":"A. Mohammed, D. Mhya","doi":"10.9734/JABB/2021/V24I630219","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I630219","url":null,"abstract":"Aims: Bambara nut seeds have been reported as a good source of food with high fiber and nutritional contents, and is consumed by persons with diabetes as one of the dietary therapy. However, the plant seeds are of different varieties and whether they might have vary biological effects on renal and liver functions is not fully known. This has prompted the study to evaluate effects of consuming different varieties of Bambara Nut seeds on liver and kidney of both diabetic and non-diabetic rats. \u0000Methodology: Four Bambara nut seeds varieties were dehulled and each heated at 60oC, cooled then grounded into flour using a mechanical grinder. The flours were used to formulate feeds which were given to different diabetic and non-diabetic rats’ groups as follows; group A (ALK01 feed), group B (ALK02 feed), group C (ALK03 feed), group D (ALK04 feed), group E (Basal feed), and group F (Normal animal’s feed). The animals were fed for 28 days then sacrifice, blood collected and serum separated and used for hepatic and renal parameters analysis. \u0000Results: The results of the study showed decrease levels of serum total protein and albumin, and elevation of AST and ALT activities as well as urea and creatinine concentrations in diabetic rats fed basal and normal diets, whereas, reverse in these parameters were recorded from diabetic rats fed varieties of Bambara nut seeds’ formulated feeds. \u0000Conclusion: Findings from the study suggest consumption of Bambara nut seeds of different varieties is safe and has ability to ameliorate diabetic hepatic and renal failure in a variety dependent manner where; ALK 04 is more likely the most effective.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78314458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-07-14DOI: 10.9734/JABB/2021/V24I630218
G. Omokhua, C. Fredrick, C. N. Okakpu
Aims: This study evaluated the influence of 11 potting media compositions on seedling growth and development of Tectona grandis. Study Design: The experiment was set up in a completely randomized design involving ten replicates. Place and Duration of Study: The study was carried out at the Department of Forestry and Wildlife Management Nursery, University of Port Harcourt, Rivers State, Nigeria, between April 2019 and April 2020. Methodology: Ten (10) seedlings per sowing media including topsoil, topsoil and cow dung (2:1, 3:1), topsoil and poultry dung (2:1, 3:1), topsoil and pig dung (2.1, 3:1), topsoil and sawdust (2.1, 3:1), topsoil and mushroom substrate (2.1, 3:1) were selected and transplanted into polybags. Growth parameters (seedling height, root collar diameter, leaf number and biomass (shoot dry weight (SDW), root dry weight (RDW) and total dry weight (TDW)) were determined and subjected to analysis of variance. Results: Seedlings of T. grandis displayed significant differences (p ≤ 0.05) in height, root collar diameter, leaf number and biomass. Among treatments, tallest seedlings were observed in mixture of topsoil and poultry dung (3:1), at months 1 to 3 and in mixture of topsoil and cow dung (3:1) at months 4 and 5, highest root collar diameter was observed in mixture of topsoil and poultry dung (3:1) and highest leaf number was recorded in mixture of topsoil and poultry dung (2:1 and 3:1) and topsoil and cow dung (2:1 and 3:1) at months 1 to 5. The highest SDW was evident in mixture of topsoil and poultry dung (2:1) while highest RDW and TDW were observed in mixture of topsoil and cow dung (3:1). Lowest height, diameter, leaf number and biomass were recorded in topsoil (control) at months 1 to 5. Conclusion: The results revealed that among the tested mixtures, topsoil and poultry dung and topsoil and cow dung mixtures were the best for seedlings growth of T. grandis.
{"title":"Seedling Growth of Tectona grandis using Different Potting Mixture","authors":"G. Omokhua, C. Fredrick, C. N. Okakpu","doi":"10.9734/JABB/2021/V24I630218","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I630218","url":null,"abstract":"Aims: This study evaluated the influence of 11 potting media compositions on seedling growth and development of Tectona grandis. \u0000Study Design: The experiment was set up in a completely randomized design involving ten replicates. \u0000Place and Duration of Study: The study was carried out at the Department of Forestry and Wildlife Management Nursery, University of Port Harcourt, Rivers State, Nigeria, between April 2019 and April 2020. \u0000Methodology: Ten (10) seedlings per sowing media including topsoil, topsoil and cow dung (2:1, 3:1), topsoil and poultry dung (2:1, 3:1), topsoil and pig dung (2.1, 3:1), topsoil and sawdust (2.1, 3:1), topsoil and mushroom substrate (2.1, 3:1) were selected and transplanted into polybags. Growth parameters (seedling height, root collar diameter, leaf number and biomass (shoot dry weight (SDW), root dry weight (RDW) and total dry weight (TDW)) were determined and subjected to analysis of variance. \u0000Results: Seedlings of T. grandis displayed significant differences (p ≤ 0.05) in height, root collar diameter, leaf number and biomass. Among treatments, tallest seedlings were observed in mixture of topsoil and poultry dung (3:1), at months 1 to 3 and in mixture of topsoil and cow dung (3:1) at months 4 and 5, highest root collar diameter was observed in mixture of topsoil and poultry dung (3:1) and highest leaf number was recorded in mixture of topsoil and poultry dung (2:1 and 3:1) and topsoil and cow dung (2:1 and 3:1) at months 1 to 5. The highest SDW was evident in mixture of topsoil and poultry dung (2:1) while highest RDW and TDW were observed in mixture of topsoil and cow dung (3:1). Lowest height, diameter, leaf number and biomass were recorded in topsoil (control) at months 1 to 5. \u0000Conclusion: The results revealed that among the tested mixtures, topsoil and poultry dung and topsoil and cow dung mixtures were the best for seedlings growth of T. grandis.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84905542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aim: This study aimed at investigating the anti-microbial, anti-inflammatory, and analgesic properties of rhizome extracts of Curcuma longa (Turmeric). Methodology: Extraction of biochemical constituents of the 150 g of the turmeric rhizomes powder was carried out successively using hexane, ethyl acetate, methanol, and water as solvents. The antimicrobial potentials of the different fractions of the extract were tested on clinical bacterial including Staphylococcus aureus, Streptococcus pneumonia, Klebsiella pneumonia and Pseudomonas aeruginosa using agar well diffusion method at concentrations of 400 mg/ml, 200 mg/ml, 100 mg/ml, 50 mg/ml, and 25 mg/ml. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using agar dilution technique. For the anti-inflammatory effects of the extract of C. longa, albino rats were treated with methanolic extract (250, 500, and 1000 mg/kg body weight) and diclofenac as control, after which they were injected with egg albumin on the right hind paw to induce inflammation. The swelling degree of the injected paw was measured. The analgesic effect of C. longa methanolic extract on hot plate-induced pain was also investigated in albino rats, and the Index of response latency was recorded. Results: The biochemical assay showed that C. longa extract contained Alkaloids, Saponins, Tannins, Flavonoids, Carbohydrate, Steroids, Terpenes, Anthraquinone, and Cardiac Glycosides, with Methanol and aqueous extracts having the most biochemical constituents. The antibacterial susceptibility screening showed that K.pneumonia was the most susceptible organism, having the highest zone of inhibition of 18 mm for ethyl acetate extract at the concentration of 400 mg/ml. This was followed by S. pneumonia, S.aureus and P. aeruginosa with inhibition zone of 13, 12 and 10 mm respectively. The MIC for K.pneumonia and S.aureus were both 200 mg/ml, while that of P. aeruginosa and S. pneumonia were both 400 mg/ml. The paw size of rats treated with increasing doses of the extract and diclofenac significantly decreased with time. The concentration of 250 mg/kg had the lowest paw volume of 4.39±0.36mm while 1000 mg/kg recorded 5.18±0.28 as the highest, at 180 minute. Conclusion: Based on the result obtained, it can be concluded that Curcuma longa Rhizome extract possess anti-microbial, anti-inflammatory, and analgesic properties.
{"title":"The Antimicrobial, Anti Inflammatory and Analgesic Activities of the Rhizome Extract of Curcuma longa L. (Turmeric)","authors":"Ogbonna Abigail Ify, Adejube Glory Raphael, Okoye Clifford Tochukwu, Ogbonna Ugoy Sonia Amarachi, N. Ikechukwu, Madu Josephine Madukaihe, Yakubu Thomas, Ogbonna Chike Innocent","doi":"10.9734/JABB/2021/V24I630217","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I630217","url":null,"abstract":"Aim: This study aimed at investigating the anti-microbial, anti-inflammatory, and analgesic properties of rhizome extracts of Curcuma longa (Turmeric). \u0000Methodology: Extraction of biochemical constituents of the 150 g of the turmeric rhizomes powder was carried out successively using hexane, ethyl acetate, methanol, and water as solvents. The antimicrobial potentials of the different fractions of the extract were tested on clinical bacterial including Staphylococcus aureus, Streptococcus pneumonia, Klebsiella pneumonia and Pseudomonas aeruginosa using agar well diffusion method at concentrations of 400 mg/ml, 200 mg/ml, 100 mg/ml, 50 mg/ml, and 25 mg/ml. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined using agar dilution technique. For the anti-inflammatory effects of the extract of C. longa, albino rats were treated with methanolic extract (250, 500, and 1000 mg/kg body weight) and diclofenac as control, after which they were injected with egg albumin on the right hind paw to induce inflammation. The swelling degree of the injected paw was measured. The analgesic effect of C. longa methanolic extract on hot plate-induced pain was also investigated in albino rats, and the Index of response latency was recorded. \u0000Results: The biochemical assay showed that C. longa extract contained Alkaloids, Saponins, Tannins, Flavonoids, Carbohydrate, Steroids, Terpenes, Anthraquinone, and Cardiac Glycosides, with Methanol and aqueous extracts having the most biochemical constituents. The antibacterial susceptibility screening showed that K.pneumonia was the most susceptible organism, having the highest zone of inhibition of 18 mm for ethyl acetate extract at the concentration of 400 mg/ml. This was followed by S. pneumonia, S.aureus and P. aeruginosa with inhibition zone of 13, 12 and 10 mm respectively. The MIC for K.pneumonia and S.aureus were both 200 mg/ml, while that of P. aeruginosa and S. pneumonia were both 400 mg/ml. The paw size of rats treated with increasing doses of the extract and diclofenac significantly decreased with time. The concentration of 250 mg/kg had the lowest paw volume of 4.39±0.36mm while 1000 mg/kg recorded 5.18±0.28 as the highest, at 180 minute. \u0000Conclusion: Based on the result obtained, it can be concluded that Curcuma longa Rhizome extract possess anti-microbial, anti-inflammatory, and analgesic properties.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79857243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-18DOI: 10.9734/jabb/2021/v24i530213
K. Nadia, A. Koutoua, Y. Konan, Kassi Amalan Angèle, Soro Dogniméton, Kouakou Tano Hilaire, K. Justin
Maize (Zea mays L.) seeds irradiated with gamma or not from the fourth self-fertilisation cycle were selected for tissue culture. For this purpose, MS medium supplemented with 30 g.L-1 sucrose, 100 mg.L-1 casein hydrolysate, 100 mg.L-1 myo-inositol and 6 g.L-1 agar was used. For this purpose, three auxins type (2.4-dichlorophenoxyacetic acid (2.4-D), 2-methoxy-3.6-dichlorobenzoic acid (Dicamba) and Indole-3-Acetic Acid (AIA)) and explants (root, epicotyl and leaf) were tested. The results showed that 2.4-D more precisely at 3.5 mg.L-1 was the best auxin for callus induction in the different maize mutants studied. The induction rate, dry matter weight and water content of callus varied according to the type, age, explant position and the maize mutants studied (control, 200 and 300 grays). Thus, the 7-day root and more precisely its basal part was better for callogenesis. In addition, mutants of the 200 grays dose were more expressive in the ability to induce callus in EV8728maize variety.
{"title":"Effect of Hormonal Regime and Explant Type on Cell Clusters Expression of Maize Mutants (Zea Mays L) Derived from Gamma Irradiated Seeds of Ev8728 variety","authors":"K. Nadia, A. Koutoua, Y. Konan, Kassi Amalan Angèle, Soro Dogniméton, Kouakou Tano Hilaire, K. Justin","doi":"10.9734/jabb/2021/v24i530213","DOIUrl":"https://doi.org/10.9734/jabb/2021/v24i530213","url":null,"abstract":"Maize (Zea mays L.) seeds irradiated with gamma or not from the fourth self-fertilisation cycle were selected for tissue culture. For this purpose, MS medium supplemented with 30 g.L-1 sucrose, 100 mg.L-1 casein hydrolysate, 100 mg.L-1 myo-inositol and 6 g.L-1 agar was used. For this purpose, three auxins type (2.4-dichlorophenoxyacetic acid (2.4-D), 2-methoxy-3.6-dichlorobenzoic acid (Dicamba) and Indole-3-Acetic Acid (AIA)) and explants (root, epicotyl and leaf) were tested. The results showed that 2.4-D more precisely at 3.5 mg.L-1 was the best auxin for callus induction in the different maize mutants studied. The induction rate, dry matter weight and water content of callus varied according to the type, age, explant position and the maize mutants studied (control, 200 and 300 grays). Thus, the 7-day root and more precisely its basal part was better for callogenesis. In addition, mutants of the 200 grays dose were more expressive in the ability to induce callus in EV8728maize variety.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"105 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85901767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-04DOI: 10.9734/JABB/2021/V24I530212
U. Georgewill, Elias Adikwu
The search for newer antimalarial drug combinations is on the front burner due to rising Plasmodium resistance to some currently used antimalarial drugs. This study examined the antiplasmodial activity of sulfadoxine/pyrimethamine/doxycycline (S/P/D) on mice infected with Plasmodium berghei (P. berghei). Swiss albino mice (25-30 g) inoculated with P. bergei (1x10 7 ) were treated with D (2.2 mg/kg), S/P (21.4/10.7 mg/kg), and S/P/D for 4 days. The positive and negative controls were treated with normal saline (0.2 ml) and chloroquine (CQ) (10 mg/kg) for 4 days, respectively. After treatment, blood samples were collected and assessed for parasitemia levels and biochemical parameters. The mice were observed for mean survival time (MST). D, S/P, S/P/D and CQ significantly decreased parasitemia in the curative, prophylactic and suppressive tests at p<0.05; p<0.01, p<0.001 and p<0.001, respectively when compared to negative control. In the curative study, 55.9%, 65.1%, and 81.7% parasitemia inhibitions were produced by D, S/P and S/P/D, respectively whereas CQ produced 75.6 % parasitemia inhibition. D, S/P and S/P/D significantly prolonged MST at p<0.05, p<0.01 and p<0.001 respectively when compared to negative control. Original Research Article Georgewill and Adikwu; JABB, 24(5): 1-8, 2021; Article no.JABB.67463 2 Altered serum biochemical markers in P. berghei infected mice were marked by significantly (p<0.001) decreased packed cell volume, red blood cells, hemoglobin, high density lipoprotein cholesterol levels with significantly (p<0.001) increased cholesterol, white blood cells, total cholesterol, low-density lipoprotein cholesterol and triglyceride levels when compared to control. However, D, S/P and S/P/D significantly restored the aforementioned markers at p<0.05, p<0.01 and p<0.001, respectively when compared to negative control. S/P/D may be used as an antimalarial drug.
{"title":"In-vivo Antiplasmodial Activity of Sulfadoxine/Pyrimethamine/Doxycycline on Plasmodium berghei Infected Mice","authors":"U. Georgewill, Elias Adikwu","doi":"10.9734/JABB/2021/V24I530212","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I530212","url":null,"abstract":"The search for newer antimalarial drug combinations is on the front burner due to rising Plasmodium resistance to some currently used antimalarial drugs. This study examined the antiplasmodial activity of sulfadoxine/pyrimethamine/doxycycline (S/P/D) on mice infected with Plasmodium berghei (P. berghei). Swiss albino mice (25-30 g) inoculated with P. bergei (1x10 7 ) were treated with D (2.2 mg/kg), S/P (21.4/10.7 mg/kg), and S/P/D for 4 days. The positive and negative controls were treated with normal saline (0.2 ml) and chloroquine (CQ) (10 mg/kg) for 4 days, respectively. After treatment, blood samples were collected and assessed for parasitemia levels and biochemical parameters. The mice were observed for mean survival time (MST). D, S/P, S/P/D and CQ significantly decreased parasitemia in the curative, prophylactic and suppressive tests at p<0.05; p<0.01, p<0.001 and p<0.001, respectively when compared to negative control. In the curative study, 55.9%, 65.1%, and 81.7% parasitemia inhibitions were produced by D, S/P and S/P/D, respectively whereas CQ produced 75.6 % parasitemia inhibition. D, S/P and S/P/D significantly prolonged MST at p<0.05, p<0.01 and p<0.001 respectively when compared to negative control. Original Research Article Georgewill and Adikwu; JABB, 24(5): 1-8, 2021; Article no.JABB.67463 2 Altered serum biochemical markers in P. berghei infected mice were marked by significantly (p<0.001) decreased packed cell volume, red blood cells, hemoglobin, high density lipoprotein cholesterol levels with significantly (p<0.001) increased cholesterol, white blood cells, total cholesterol, low-density lipoprotein cholesterol and triglyceride levels when compared to control. However, D, S/P and S/P/D significantly restored the aforementioned markers at p<0.05, p<0.01 and p<0.001, respectively when compared to negative control. S/P/D may be used as an antimalarial drug.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"91 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2021-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79420412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-06-04DOI: 10.9734/JABB/2021/V24I430211
Enas N. Danial, Walaa A Majrashi, Ahlam O. Bin Afif, Ebtehal S Alamri, Entesar M. Alhatimi, Nowayer J. Alghamdi
Environmental pollution of heavy metals is increasingly becoming a problem and has become of great concern due to the adverse effects it is causing around the world. These inorganic pollutants are being discarded in our waters, soils and into the atmosphere due to the rapidly growing agriculture and metal industries, improper waste disposal, fertilizers, and pesticides. Pollution in industrial areas is a serious environmental concern. Wastewater containing biotoxic substances of heavy metals in the ecosystem is one of the most important environmental and health challenges in our society. Hence, there is a growing need for the development of novel, efficient, eco-friendly, and cost-effective approach for the remediation of inorganic metals (Cr, Hg, Cd, and Pb) released into the environment and to safeguard the ecosystem. Mercury (Hg), Chromium (Cr), Cadmium (Cd), Review Article Danial et al.; JABB, 24(4): 48-72, 2021; Article no.JABB.68532 49 and lead (Pb) are known to cause damage to living organisms, including human beings. In this regard, recent advances in microbes-base heavy metal have propelled bioremediation as a prospective alternative to conventional techniques. Heavy metals are nonbiodegradable and could be toxic to microbes. Several microorganisms have evolved to develop detoxification mechanisms to counter the toxic effects of these inorganic metals. Several marine bacteria highly resistant and capable of growing at higher concentrations of Hg, Cr, Cd and Pb and to evaluate their potential to detoxify. Their detoxification efficiency for Hg, Cr, Cd and Pb indicates good potential for application in bioremediation of toxic heavy metals.
{"title":"Cytotoxicity and Bioremediation of Heavy Metals by Highly Resistant Marine Bacteria","authors":"Enas N. Danial, Walaa A Majrashi, Ahlam O. Bin Afif, Ebtehal S Alamri, Entesar M. Alhatimi, Nowayer J. Alghamdi","doi":"10.9734/JABB/2021/V24I430211","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I430211","url":null,"abstract":"Environmental pollution of heavy metals is increasingly becoming a problem and has become of great concern due to the adverse effects it is causing around the world. These inorganic pollutants are being discarded in our waters, soils and into the atmosphere due to the rapidly growing agriculture and metal industries, improper waste disposal, fertilizers, and pesticides. Pollution in industrial areas is a serious environmental concern. Wastewater containing biotoxic substances of heavy metals in the ecosystem is one of the most important environmental and health challenges in our society. Hence, there is a growing need for the development of novel, efficient, eco-friendly, and cost-effective approach for the remediation of inorganic metals (Cr, Hg, Cd, and Pb) released into the environment and to safeguard the ecosystem. Mercury (Hg), Chromium (Cr), Cadmium (Cd), Review Article Danial et al.; JABB, 24(4): 48-72, 2021; Article no.JABB.68532 49 and lead (Pb) are known to cause damage to living organisms, including human beings. In this regard, recent advances in microbes-base heavy metal have propelled bioremediation as a prospective alternative to conventional techniques. Heavy metals are nonbiodegradable and could be toxic to microbes. Several microorganisms have evolved to develop detoxification mechanisms to counter the toxic effects of these inorganic metals. Several marine bacteria highly resistant and capable of growing at higher concentrations of Hg, Cr, Cd and Pb and to evaluate their potential to detoxify. Their detoxification efficiency for Hg, Cr, Cd and Pb indicates good potential for application in bioremediation of toxic heavy metals.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"2 1","pages":"48-72"},"PeriodicalIF":0.0,"publicationDate":"2021-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83243532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-25DOI: 10.9734/JABB/2021/V24I430209
Paul Ndip Besong, T. R. Kinge
Fruits play an essential role in human nutrition by contributing the necessary growth factors like essential minerals and vitamins in human daily diet maintaining a good and normal health. But rot diseases caused by fungi cause severe losses of agricultural and horticultural crops every year. This work aimed to study fungi diversity on some fruits and carry out biological control using two plant extracts. A total of 17 infected fruit samples were collected from two local markets, small pieces of infected parts were inoculated on prepared plates of Potato Dextrose Agar. Incubation was done for 7 days and pure cultures were made, and pure isolated fungi were identified according to the recommended references. Ethanolic leaf extracts of Ocimum gratissimum and Moringa oleifera were evaluated for in vitro antifungal activities on Aspergillus and Fusarium species isolated from spoilt tomatoes and banana using the Agar Dilution Method. Eleven different fungi species comprising nine genera were isolated from the 17 fruits collected from the Nkwen and main markets of Bamenda. The fungi were identified as Saccharomyces cerevisiae, Aspergillus niger, Penicillium digitatum, Mucor sp, Fusarium sp, Mucor racemosus, Alternaria alternata, Colletotrichum sp, Nodulisporium sp, Fusarium oxysporum and Aspergillus flavus. There was some diversity in isolation frequency of the fungi from the fruits. Aspergillus, Penicillium, and Fusarium were the most Original Research Article Besong and Kinge; JABB, 24(4): 24-38, 2021; Article no.JABB.68075 25 common genera that colonized the fruits, with Aspergillus sp. found to be the most dominant fungi responsible for extensive damage of fruits. Ocimum gratissimum and Moringa oleifera leaf extracts had inhibitory activities on the test fungi. The diversity of the fungi identified in this study could be regarded as the most common causes of post-harvest deterioration of fruits. The findings of this study bring further evidence that Moringa oleifera and Ocimum gratissimum leaves extracts have the potential of becoming powerful and safe alternative means of fungi control on fruits instead of the harmful, expensive, environmentally unfriendly chemical fungicides.
{"title":"Fungi Diversity on Some Fruits and Biological Control using Two Plants Extracts","authors":"Paul Ndip Besong, T. R. Kinge","doi":"10.9734/JABB/2021/V24I430209","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I430209","url":null,"abstract":"Fruits play an essential role in human nutrition by contributing the necessary growth factors like essential minerals and vitamins in human daily diet maintaining a good and normal health. But rot diseases caused by fungi cause severe losses of agricultural and horticultural crops every year. This work aimed to study fungi diversity on some fruits and carry out biological control using two plant extracts. A total of 17 infected fruit samples were collected from two local markets, small pieces of infected parts were inoculated on prepared plates of Potato Dextrose Agar. Incubation was done for 7 days and pure cultures were made, and pure isolated fungi were identified according to the recommended references. Ethanolic leaf extracts of Ocimum gratissimum and Moringa oleifera were evaluated for in vitro antifungal activities on Aspergillus and Fusarium species isolated from spoilt tomatoes and banana using the Agar Dilution Method. Eleven different fungi species comprising nine genera were isolated from the 17 fruits collected from the Nkwen and main markets of Bamenda. The fungi were identified as Saccharomyces cerevisiae, Aspergillus niger, Penicillium digitatum, Mucor sp, Fusarium sp, Mucor racemosus, Alternaria alternata, Colletotrichum sp, Nodulisporium sp, Fusarium oxysporum and Aspergillus flavus. There was some diversity in isolation frequency of the fungi from the fruits. Aspergillus, Penicillium, and Fusarium were the most Original Research Article Besong and Kinge; JABB, 24(4): 24-38, 2021; Article no.JABB.68075 25 common genera that colonized the fruits, with Aspergillus sp. found to be the most dominant fungi responsible for extensive damage of fruits. Ocimum gratissimum and Moringa oleifera leaf extracts had inhibitory activities on the test fungi. The diversity of the fungi identified in this study could be regarded as the most common causes of post-harvest deterioration of fruits. The findings of this study bring further evidence that Moringa oleifera and Ocimum gratissimum leaves extracts have the potential of becoming powerful and safe alternative means of fungi control on fruits instead of the harmful, expensive, environmentally unfriendly chemical fungicides.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"86 14 1","pages":"24-38"},"PeriodicalIF":0.0,"publicationDate":"2021-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84008261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-05-25DOI: 10.9734/JABB/2021/V24I430210
U. A. Adekola, I. Eiroboyi, Y. Yerima, T. E. B. Akinmoji, L. O. Uti
The need for an environmentally friendly energy source in the world has led to major diversification in renewable energy. Biogas provides a renewable energy source that will replace fossil fuel inevitably. The experiment was carried out using a self-designed laboratory-scale anaerobic biogas digester. The study was carried out at room temperature from 25 31°C for 20 days using corn stalk as the main substrate while Pig manure and eggshell were used as co-substrates. The findings showed that the biogas produced from the sample containing a blend of corn stalk, Pig manure, and eggshell resulted in higher biogas volume than the sample containing corn stalk and eggshell, corn stalk, and pig manure as well as the sample containing only corn stalk. This implies that the use of the corn stalk blend is a source of renewable energy. Thus, ensuring the sustainability of biogas production in the future. Original Research Article Adekola et al.; JABB, 24(4): 39-47, 2021; Article no.JABB.68458 40
世界上对环境友好型能源的需要导致了可再生能源的重大多样化。沼气是一种可再生能源，将不可避免地取代化石燃料。实验采用自行设计的实验室规模厌氧沼气池进行。以玉米秸秆为主基质，猪粪和蛋壳为辅基质，在25 ~ 31℃的室温条件下进行了为期20 d的试验。结果表明，玉米秸秆-猪粪-蛋壳混合样品产生的沼气量高于玉米秸秆-蛋壳混合样品、玉米秸秆-猪粪混合样品和仅含玉米秸秆的样品。这意味着使用玉米秸秆混合物是一种可再生能源。从而确保未来沼气生产的可持续性。Adekola et al.;;Jabb, 24(4): 39-47, 2021;文章no.JABB。68458年40
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Pub Date : 2021-05-19DOI: 10.9734/JABB/2021/V24I430207
A. R. Alhassan, C. Saba, S. W. Kpordze
Aim: This research was carried out to determine the prevalence of Salmonella species from smoked fish sold in the three major markets in Tamale Metropolis and examined the isolates resistance patterns to various antibiotics. Study Design: The study was in two parts which include administering questionnaires and collecting samples. The second part was the laboratory analysis to detect Salmonella species from the collected samples. Place and Duration of Study: The study was carried out in the Spanish laboratory (microbiology section) of the Department of Biotechnology, Faculty of Biosciences, of the University for Development Studies. Methodology: A total of one hundred samples were examined. The samples were enriched on buffered peptone water and inoculated on Modified Semi-Solid Rappaport Vassiliadis. Xylose Lysine Deoxycholate was used to identify the bacteria, and Simons Citrate agar was used for the biochemical test. The antibiotic test was done by using the Kirby-Bauer disc diffusion test. Original Research Article Alhassan et al.; JABB, 24(4): 1-11, 2021; Article no. JABB.68023 2 Results: After the laboratory analysis, 67 (67%) out of the 100 samples were confirmed to be positive for Salmonella species of which Cut fish (11.9%), Redfish (11.9%), Mudfish (11.9%), and Chale fish (11.9%) recorded the highest contamination, and among the three markets, Central market had the highest fish contamination. The resistance patterns of the isolates to the various antibiotics used were; Ciprofloxacin (2.98%), Ceftriaxone (34.32%), Ampicillin (83.58%), Doxycycline (88.05%), Amoxicillin/Clavulanic acid (91.04%), and Oxytetracycline (92.53%). Conclusion: This study revealed that smoked fish sold in the Tamale Metropolis contained Salmonella positive that can cause food poisoning and other gastrointestinal problems.
目的:对塔梅尔市3个主要市场销售的熏鱼中沙门氏菌的流行情况进行调查，并检测其对各种抗生素的耐药性。研究设计:本研究分为问卷调查和样本收集两部分。第二部分是实验室分析，从采集的样品中检测沙门氏菌的种类。研究地点和时间:该研究在发展研究大学生物科学学院生物技术系的西班牙实验室(微生物科)进行。方法:共检查100个样本。将样品用缓冲蛋白胨水富集，接种于改性半固体Rappaport Vassiliadis上。采用木糖赖氨酸脱氧胆酸酯进行细菌鉴定，柠檬酸西蒙斯琼脂进行生化试验。抗生素试验采用Kirby-Bauer盘扩散试验。Alhassan et al.;Jabb, 24(4): 1- 11,2021;货号。结果:经实验室分析，100份样品中有67份(67%)检出沙门氏菌，其中切鱼(11.9%)、红鱼(11.9%)、泥鱼(11.9%)和Chale鱼(11.9%)的污染最高，其中中环市场的污染最高。分离株对常用抗生素的耐药模式为:环丙沙星(2.98%)、头孢曲松(34.32%)、氨苄西林(83.58%)、多西环素(88.05%)、阿莫西林/克拉维酸(91.04%)、土霉素(92.53%)。结论:本研究发现，在Tamale Metropolis出售的熏鱼含有沙门氏菌，可引起食物中毒和其他胃肠道问题。
{"title":"Prevalence and Antibiotic Susceptibility Pattern of Salmonella Species","authors":"A. R. Alhassan, C. Saba, S. W. Kpordze","doi":"10.9734/JABB/2021/V24I430207","DOIUrl":"https://doi.org/10.9734/JABB/2021/V24I430207","url":null,"abstract":"Aim: This research was carried out to determine the prevalence of Salmonella species from smoked fish sold in the three major markets in Tamale Metropolis and examined the isolates resistance patterns to various antibiotics. Study Design: The study was in two parts which include administering questionnaires and collecting samples. The second part was the laboratory analysis to detect Salmonella species from the collected samples. Place and Duration of Study: The study was carried out in the Spanish laboratory (microbiology section) of the Department of Biotechnology, Faculty of Biosciences, of the University for Development Studies. Methodology: A total of one hundred samples were examined. The samples were enriched on buffered peptone water and inoculated on Modified Semi-Solid Rappaport Vassiliadis. Xylose Lysine Deoxycholate was used to identify the bacteria, and Simons Citrate agar was used for the biochemical test. The antibiotic test was done by using the Kirby-Bauer disc diffusion test. Original Research Article Alhassan et al.; JABB, 24(4): 1-11, 2021; Article no. JABB.68023 2 Results: After the laboratory analysis, 67 (67%) out of the 100 samples were confirmed to be positive for Salmonella species of which Cut fish (11.9%), Redfish (11.9%), Mudfish (11.9%), and Chale fish (11.9%) recorded the highest contamination, and among the three markets, Central market had the highest fish contamination. The resistance patterns of the isolates to the various antibiotics used were; Ciprofloxacin (2.98%), Ceftriaxone (34.32%), Ampicillin (83.58%), Doxycycline (88.05%), Amoxicillin/Clavulanic acid (91.04%), and Oxytetracycline (92.53%). Conclusion: This study revealed that smoked fish sold in the Tamale Metropolis contained Salmonella positive that can cause food poisoning and other gastrointestinal problems.","PeriodicalId":15023,"journal":{"name":"Journal of Advances in Biology & Biotechnology","volume":"21 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2021-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73704146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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