Pub Date : 2022-01-01DOI: 10.7324/jabb.2021.100106
S. Vyacheslav, Alikulov Begali, Davranov Kakhramon, I. Zafar
{"title":"Bacterial endophytes from halophyte black saxaul (Haloxylon aphyllum Minkw.) and their plant growth-promoting properties","authors":"S. Vyacheslav, Alikulov Begali, Davranov Kakhramon, I. Zafar","doi":"10.7324/jabb.2021.100106","DOIUrl":"https://doi.org/10.7324/jabb.2021.100106","url":null,"abstract":"","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71217682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.7324/jabb.2021.100112
Bhamare Mukta R., Thakur Hemantkumar A., Chavan Jaykumar J.
The present study aimed to optimize the in vitro direct shoot organogenesis system for propagation, in vitro flowering, and genetic fidelity analysis of micropropagated clones of Ceropegia lawii. The efficiency of various plant growth regulator treatments was evaluated during various regeneration stages. Direct shoot organogenesis cultures were established through stem nodal buds on Murashige and Skoog (MS) medium supplemented by 6-benzylaminopurine (BAP, 0.5 mg/l). Maximum frequency of shoot induction and multiplication (98.5%) with 14 ± 0.5 shoots per explant was attained through the augmentation of BAP (2.0 mg/l) in the MS medium. In vitro-derived shoots were rooted optimum (89.62%) with 6.9 ± 0.1 numbers of roots while transferring to a half-strength MS medium supplied with indole-3-butyric acid (1.5 mg/l). A maximum number of flowers (5.6 ± 0.1) with 91.33% induction frequency were reported when microshoots were transferred to the MS medium formulated with BAP (2.0 mg/l) in combination with sucrose (175 mM). Tissue culture-derived plantlets were adapted best in a mixture of sterile soil, sand, and coco peat (1:2:1) with 88% of survival rate. Inter-simple sequence repeat analysis of regenerated plantlets revealed 99.33% genetic integrity of in vitro regenerated clones when compared with the donor plant. The findings of the present study reveals that the direct shoot organogenesis approach produces a large number of genetically stable plantlets of C. lawii which helps in its conservation and fulfills further industrial necessities.
{"title":"Efficient plant regeneration and molecular marker-based genetic integrity analysis of Ceropegia lawii Hook: An endemic and endangered herb","authors":"Bhamare Mukta R., Thakur Hemantkumar A., Chavan Jaykumar J.","doi":"10.7324/jabb.2021.100112","DOIUrl":"https://doi.org/10.7324/jabb.2021.100112","url":null,"abstract":"The present study aimed to optimize the in vitro direct shoot organogenesis system for propagation, in vitro flowering, and genetic fidelity analysis of micropropagated clones of Ceropegia lawii. The efficiency of various plant growth regulator treatments was evaluated during various regeneration stages. Direct shoot organogenesis cultures were established through stem nodal buds on Murashige and Skoog (MS) medium supplemented by 6-benzylaminopurine (BAP, 0.5 mg/l). Maximum frequency of shoot induction and multiplication (98.5%) with 14 ± 0.5 shoots per explant was attained through the augmentation of BAP (2.0 mg/l) in the MS medium. In vitro-derived shoots were rooted optimum (89.62%) with 6.9 ± 0.1 numbers of roots while transferring to a half-strength MS medium supplied with indole-3-butyric acid (1.5 mg/l). A maximum number of flowers (5.6 ± 0.1) with 91.33% induction frequency were reported when microshoots were transferred to the MS medium formulated with BAP (2.0 mg/l) in combination with sucrose (175 mM). Tissue culture-derived plantlets were adapted best in a mixture of sterile soil, sand, and coco peat (1:2:1) with 88% of survival rate. Inter-simple sequence repeat analysis of regenerated plantlets revealed 99.33% genetic integrity of in vitro regenerated clones when compared with the donor plant. The findings of the present study reveals that the direct shoot organogenesis approach produces a large number of genetically stable plantlets of C. lawii which helps in its conservation and fulfills further industrial necessities.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71217724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.7324/jabb.2021.100116
Riyad Priyanka, P. Ashok, Sen Karishma, R. Heera
The current study was carried out to evaluate the potential of curcumin against the progression of atherosclerosis and cholesterol biosynthesis by incorporating the combined data of in-vivo assessments and integrative omics examinations. The high fat diet and supplementation of cholesterol powder caused significant alterations in the lipid profile as well as hypercholesterolemia indices. The induced hypercholesterolemia promoted progression of atherosclerotic plaque with the occurrence of foam cells in a bulged structure. Simultaneously, the treatments of curcumin and atorvastatin caused significant reductions in total cholesterol, low density lipoprotein cholesterol, and very low-density lipoprotein cholesterol, as well as hypercholesterolemia indices of Castelli Risk Index-I and II and atherogenic indices. Accordingly, the treatments of curcumin and atorvastatin caused significant regressions in atherogenic plaque area, total wall area, and increased lumen volume. Subsequently, molecular docking showed significant interactions of curcumin and atorvastatin with β-Hydroxy β-methylglutaryl-CoA reductase, which were depicted by bonding energy, number of H–bonds, and bond length. Accordingly, the absorption, distribution, metabolism, excretion, and toxicity and toxicity data revealed significant druggability of curcumin along with supportive analysis of Brain Or IntestinaL EstimateD-Egg prediction of gastrointestinal absorption. Thus, it can be illustrated that curcumin has significant potential to promote regression in atherosclerotic plaque and subside the cholesterol biosynthesis by inhibition of HMG-CoA reductase, as indicated by the outcomes.
{"title":"Atherosclerotic plaque regression and HMG-CoA reductase inhibition potential of curcumin: An integrative omics and in-vivo study","authors":"Riyad Priyanka, P. Ashok, Sen Karishma, R. Heera","doi":"10.7324/jabb.2021.100116","DOIUrl":"https://doi.org/10.7324/jabb.2021.100116","url":null,"abstract":"The current study was carried out to evaluate the potential of curcumin against the progression of atherosclerosis and cholesterol biosynthesis by incorporating the combined data of in-vivo assessments and integrative omics examinations. The high fat diet and supplementation of cholesterol powder caused significant alterations in the lipid profile as well as hypercholesterolemia indices. The induced hypercholesterolemia promoted progression of atherosclerotic plaque with the occurrence of foam cells in a bulged structure. Simultaneously, the treatments of curcumin and atorvastatin caused significant reductions in total cholesterol, low density lipoprotein cholesterol, and very low-density lipoprotein cholesterol, as well as hypercholesterolemia indices of Castelli Risk Index-I and II and atherogenic indices. Accordingly, the treatments of curcumin and atorvastatin caused significant regressions in atherogenic plaque area, total wall area, and increased lumen volume. Subsequently, molecular docking showed significant interactions of curcumin and atorvastatin with β-Hydroxy β-methylglutaryl-CoA reductase, which were depicted by bonding energy, number of H–bonds, and bond length. Accordingly, the absorption, distribution, metabolism, excretion, and toxicity and toxicity data revealed significant druggability of curcumin along with supportive analysis of Brain Or IntestinaL EstimateD-Egg prediction of gastrointestinal absorption. Thus, it can be illustrated that curcumin has significant potential to promote regression in atherosclerotic plaque and subside the cholesterol biosynthesis by inhibition of HMG-CoA reductase, as indicated by the outcomes.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71218087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.7324/jabb.2021.100118
L. L., Veeraraghavan V., Srihari Renuka, P. C N
Abutilon indicum (L.) is one of the traditional medicinal plants and its extract has been utilized for antidiabetic activity. Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and its occurrence is increasing fast in most countries. Peroxisome proliferator-activated receptor gamma (PPARγ) plays an important role in adipogenesis. The present study determines the effect of A. indicum methanolic leaf extract as potential antidiabetic inhibitors. The in vitro analysis was carried out by using 3T3L1 for glucose uptake assay, RIN5F cell lines for insulin secretion, hydrolysis assay to predict glycemic index, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay, and molecular interaction of the known bioactive compounds with PPARγ. The experimental results showed 100 nM insulin and 50 mM LiCl glucose uptake with 3.37 and 3.86 fold stimulation, respectively, when compared to the control, whereas the given samples of 200, 400, and 800 μg/ml showed 1.37, 1.62, and 1.85 μg/ml fold stimulation, respectively, when compared to the control. The insulin release in RIN-5f cells was observed with a positive control and crude extract and the results showed 4.67 and 2.67 μg/ml, respectively. The hydrolysis index value was found to be 53.30 and the glycemic load was 17.48 μg/ml. In the DPPH assay, the sample showed dose-dependent DPPH radical scavenging activity with an IC50 value of 99.12 μg/ml when compared to standard quercetin with an IC50 value of 1.7 μg/ml. The molecular interaction of PPARγ and active methyl trans-p-coumarate (−5.44) > methyl caffeate (−4.49) > syringic acid (−3.9) > pinellic acid (−2.62) compared with thiazolidinediones (−7.62) formed a novel type of oral antidiabetic medication that improved metabolic management in type 2 diabetic patients by increasing insulin sensitivity. The overall result shows that A. indicum (L.) is a potential indicator for sensitizing insulin secretion and strongly inhibits the release of glucagon which can be used as a therapeutic agent for treating and managing diabetes.
{"title":"Study of in vitro activity on glucose uptake of 3T3L1 cells, RIN5f cells, and glycemic index stimulation inhibitory effect of Abutilon indicum (L.) extract","authors":"L. L., Veeraraghavan V., Srihari Renuka, P. C N","doi":"10.7324/jabb.2021.100118","DOIUrl":"https://doi.org/10.7324/jabb.2021.100118","url":null,"abstract":"Abutilon indicum (L.) is one of the traditional medicinal plants and its extract has been utilized for antidiabetic activity. Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and its occurrence is increasing fast in most countries. Peroxisome proliferator-activated receptor gamma (PPARγ) plays an important role in adipogenesis. The present study determines the effect of A. indicum methanolic leaf extract as potential antidiabetic inhibitors. The in vitro analysis was carried out by using 3T3L1 for glucose uptake assay, RIN5F cell lines for insulin secretion, hydrolysis assay to predict glycemic index, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging assay, and molecular interaction of the known bioactive compounds with PPARγ. The experimental results showed 100 nM insulin and 50 mM LiCl glucose uptake with 3.37 and 3.86 fold stimulation, respectively, when compared to the control, whereas the given samples of 200, 400, and 800 μg/ml showed 1.37, 1.62, and 1.85 μg/ml fold stimulation, respectively, when compared to the control. The insulin release in RIN-5f cells was observed with a positive control and crude extract and the results showed 4.67 and 2.67 μg/ml, respectively. The hydrolysis index value was found to be 53.30 and the glycemic load was 17.48 μg/ml. In the DPPH assay, the sample showed dose-dependent DPPH radical scavenging activity with an IC50 value of 99.12 μg/ml when compared to standard quercetin with an IC50 value of 1.7 μg/ml. The molecular interaction of PPARγ and active methyl trans-p-coumarate (−5.44) > methyl caffeate (−4.49) > syringic acid (−3.9) > pinellic acid (−2.62) compared with thiazolidinediones (−7.62) formed a novel type of oral antidiabetic medication that improved metabolic management in type 2 diabetic patients by increasing insulin sensitivity. The overall result shows that A. indicum (L.) is a potential indicator for sensitizing insulin secretion and strongly inhibits the release of glucagon which can be used as a therapeutic agent for treating and managing diabetes.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71218312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.7324/jabb.2021.100127
N. Manoj, Kang Rigzin, Joon Deepali
Paper currency notes are the most commonly exchanged fomites which can be contaminated and have the potential to transmit pathogenic organisms. The presence of tuberculosis causative bacteria on the most commonly circulated Indian currency notes was investigated using loop-mediated isothermal amplification (LAMP). Out of 1,000 random samples of 10-rupee Indian currency notes, 11.3% tested positive for the presence of genomic DNA of Mycobacterium tuberculosis. It was observed that soiled/old notes are more likely than new currency notes to harbor M. tuberculosis. This study demonstrates the novel application of LAMP technique for screening fomites for the presence of pathogens.
{"title":"Screening paper currency for Mycobacterium tuberculosis using loop-mediated isothermal amplification","authors":"N. Manoj, Kang Rigzin, Joon Deepali","doi":"10.7324/jabb.2021.100127","DOIUrl":"https://doi.org/10.7324/jabb.2021.100127","url":null,"abstract":"Paper currency notes are the most commonly exchanged fomites which can be contaminated and have the potential to transmit pathogenic organisms. The presence of tuberculosis causative bacteria on the most commonly circulated Indian currency notes was investigated using loop-mediated isothermal amplification (LAMP). Out of 1,000 random samples of 10-rupee Indian currency notes, 11.3% tested positive for the presence of genomic DNA of Mycobacterium tuberculosis. It was observed that soiled/old notes are more likely than new currency notes to harbor M. tuberculosis. This study demonstrates the novel application of LAMP technique for screening fomites for the presence of pathogens.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"128 12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71218418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Iryna Bandura1 , Omoanghe S. Isikhuemhen2* , Alina Kulik1 , Nina Bisko3 , Maryna Serduik1 , Volodymyr Khareba4 , Olena Khareba4 , Iryna Ivanova1 , Olesya Priss1 , Oleksandr Tsyz4 , Serhii Makohon1 , Serhii Chausov1 1Department of Food Technology and HotelRestaurant Business, Tavria State Agrotechnological University, Melitopol, Ukraine. 2Mushroom Biology & Fungal Biotechnology Laboratory, North Carolina A&T State University, Greensboro, NC. 3M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv, Ukraine. 4Department of Vegetable Cultivation, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine.
Pub Date : 2022-01-01DOI: 10.7324/jabb.2021.100108
C. Sandip, Kashyap Briyanka, Dutta Karabi
{"title":"The ITS2 DNA sequence analysis in six species of barbin fishes with phylogenetic insights","authors":"C. Sandip, Kashyap Briyanka, Dutta Karabi","doi":"10.7324/jabb.2021.100108","DOIUrl":"https://doi.org/10.7324/jabb.2021.100108","url":null,"abstract":"","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71217479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.7324/jabb.2021.100111
Bisht Sanskriti, Singh Shatrupa, S. Madhulika, Sharma Jai Gopal
An experiment was conducted to evaluate the role of Piriformospora indica and plant growth promoting bacteria (PGPB) in mitigating salinity stress in Trigonella foenum graecum. Plants were subjected to three different levels of salinity, viz., 0, 70, and 150 mM NaCl (electrical conductivity value 0.01, 7.67, and 15.50 mS cm−1, respectively) using a completely randomized design experiment. The P. indica and PGPB showed positive effects in mitigation of salinity stress in fenugreek plants and elevated various growth responses, viz., shoot and root length, shoot and root dry weight, leaf area, and number of leaves as compared to uninoculated plants. Microbial inoculation significantly enhanced the physiological responses, viz., photosynthetic rate, stomatal conductance, transpiration and internal CO2 as compared to uninoculated plants. Biochemical aspects like carotenoids, chlorophylls, nitrogen, and protein content were also increased in the microbial inoculated plants as compared to uninoculated plants. However, PGPB was more effective than P. indica in mitigating salinity stress in fenugreek plant. The findings of this study revealed that P. indica and PGPB inoculation can help the plants to overcome the deleterious effects of salinity stress in fenugreek plants.
本试验研究了梨形孢子菌和植物生长促进菌(PGPB)对凹凹Trigonella foenum graecum盐胁迫的缓解作用。采用完全随机设计的试验方法，将植物置于0、70和150 mM NaCl(电导率分别为0.01、7.67和15.50 mS cm−1)3种不同盐度条件下。与未接种的葫芦巴植株相比，籼稻和PGPB在缓解盐胁迫方面表现出积极的效果，并提高了葫芦巴植株的各种生长响应，即茎和根长、茎和根干重、叶面积和叶数。与未接种的植株相比，接种微生物显著提高了植株的光合速率、气孔导度、蒸腾和内部CO2的生理响应。与未接种的植物相比，接种微生物的植物的类胡萝卜素、叶绿素、氮和蛋白质含量等生化指标也有所增加。然而，在葫芦巴植物中，PGPB对减轻盐胁迫的效果优于P. indica。本研究结果表明，接种P. indica和PGPB可以帮助葫芦巴植物克服盐胁迫的有害影响。
{"title":"Augmentative role of Piriformospora indica fungus and plant growth promoting bacteria in mitigating salinity stress in Trigonella foenum-graecum","authors":"Bisht Sanskriti, Singh Shatrupa, S. Madhulika, Sharma Jai Gopal","doi":"10.7324/jabb.2021.100111","DOIUrl":"https://doi.org/10.7324/jabb.2021.100111","url":null,"abstract":"An experiment was conducted to evaluate the role of Piriformospora indica and plant growth promoting bacteria (PGPB) in mitigating salinity stress in Trigonella foenum graecum. Plants were subjected to three different levels of salinity, viz., 0, 70, and 150 mM NaCl (electrical conductivity value 0.01, 7.67, and 15.50 mS cm−1, respectively) using a completely randomized design experiment. The P. indica and PGPB showed positive effects in mitigation of salinity stress in fenugreek plants and elevated various growth responses, viz., shoot and root length, shoot and root dry weight, leaf area, and number of leaves as compared to uninoculated plants. Microbial inoculation significantly enhanced the physiological responses, viz., photosynthetic rate, stomatal conductance, transpiration and internal CO2 as compared to uninoculated plants. Biochemical aspects like carotenoids, chlorophylls, nitrogen, and protein content were also increased in the microbial inoculated plants as compared to uninoculated plants. However, PGPB was more effective than P. indica in mitigating salinity stress in fenugreek plant. The findings of this study revealed that P. indica and PGPB inoculation can help the plants to overcome the deleterious effects of salinity stress in fenugreek plants.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71217835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.7324/jabb.2021.100125
Koné Adama, Adingra Ama Antoinette, Kambiré Ollo, KOFFI-NEVRY Amenan Rose
Adama KONE1,2*, Ama Antoinette ADINGRA2, Ollo KAMBIRE3 , Amenan Rose KOFFI-NEVRY1 1Laboratory of Biotechnology and Food Microbiology, Departement of Food Science, Nangui Abrogoua University, 02 BP 801 Abidjan, Côte d’Ivoire. 2Laboratory of Microbiology,Unit Research Océanologic, BP V 18 Abidjan, Côte d’Ivoire. 3Department of Biochemistry and Genetics, Peleforo Gon Coulibaly University, BP 1328 Korhogo, Côte d’Ivoire.
Adama KONE1,2*，Ama Antoinette ADINGRA2，Ollo KAMBIRE3，Amenan Rose KOFFI-NEVRY1生物技术与食品微生物学研究所，南圭阿布罗瓜大学食品科学系，02 BP 801阿比让，科特迪瓦。2微生物实验室，眼科研究单位，BP V 18阿比让，科特迪瓦。3生物化学和遗传学系，Pelefoo Gon Coulibaly大学，BP 1328 Korhogo，科特迪瓦。
{"title":"Prevalence of Klebsiella pneumoniae isolated from public universities’ restaurants of Abidjan, Côte d’Ivoire","authors":"Koné Adama, Adingra Ama Antoinette, Kambiré Ollo, KOFFI-NEVRY Amenan Rose","doi":"10.7324/jabb.2021.100125","DOIUrl":"https://doi.org/10.7324/jabb.2021.100125","url":null,"abstract":"Adama KONE1,2*, Ama Antoinette ADINGRA2, Ollo KAMBIRE3 , Amenan Rose KOFFI-NEVRY1 1Laboratory of Biotechnology and Food Microbiology, Departement of Food Science, Nangui Abrogoua University, 02 BP 801 Abidjan, Côte d’Ivoire. 2Laboratory of Microbiology,Unit Research Océanologic, BP V 18 Abidjan, Côte d’Ivoire. 3Department of Biochemistry and Genetics, Peleforo Gon Coulibaly University, BP 1328 Korhogo, Côte d’Ivoire.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71218147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The purpose of this study is to determine the antioxidant activity, antiproliferation, apoptosis, and cell cycle arrest induced by Coleus tuberosus flesh and peel extracted with ethanol. The antioxidant activity was determined using the 1,1-diphenylpricrylhydrazyl and cellular antioxidant assays, and the antiproliferative activity was determined using the 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Flow cytometry was used to assess cell cycle arrest, and acridine orange-ethidium bromide staining was used to assess apoptosis-induced ability. The results show that the C. tuberosus peel extract has higher antioxidant activity than the flesh extract. Coleus tuberosus flesh ethanol extract (CFEE) and C. tuberosus peel ethanol extracts’ (CPEE) antioxidant activities inhibitory concentration 50 (IC50) were 1290.00±1.58g/ml and 310.97±0.32μg/ ml, respectively. The C. tuberosus peel extract has greater antiproliferative activity than the flesh extract. The C. tuberosus flesh and peel extracts had antiproliferative activities (IC50) of 651.35±4.24 and 366.41±3.52μg/ml, respectively. The flesh and peel extracts cause apoptosis in HeLa cells. Cell cycle arrest in sub-G1 (M) and cell cycle inhibition in G0-G1 are caused by the C. tuberosus peel extract. According to this study, the CFEE and CPEEs have the potential to be a source of natural antioxidants and antiproliferation of cervical cancer.
{"title":"Antioxidative, antiproliferative, and apoptosis effect of Coleus tuberosus flesh and peel ethanol extracts on cervical cancer cell lines","authors":"Nugraheni Mutiara, Windarwati Windarwati, Lastariwati Badraningsih","doi":"10.7324/jabb.2021.100120","DOIUrl":"https://doi.org/10.7324/jabb.2021.100120","url":null,"abstract":"The purpose of this study is to determine the antioxidant activity, antiproliferation, apoptosis, and cell cycle arrest induced by Coleus tuberosus flesh and peel extracted with ethanol. The antioxidant activity was determined using the 1,1-diphenylpricrylhydrazyl and cellular antioxidant assays, and the antiproliferative activity was determined using the 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Flow cytometry was used to assess cell cycle arrest, and acridine orange-ethidium bromide staining was used to assess apoptosis-induced ability. The results show that the C. tuberosus peel extract has higher antioxidant activity than the flesh extract. Coleus tuberosus flesh ethanol extract (CFEE) and C. tuberosus peel ethanol extracts’ (CPEE) antioxidant activities inhibitory concentration 50 (IC50) were 1290.00±1.58g/ml and 310.97±0.32μg/ ml, respectively. The C. tuberosus peel extract has greater antiproliferative activity than the flesh extract. The C. tuberosus flesh and peel extracts had antiproliferative activities (IC50) of 651.35±4.24 and 366.41±3.52μg/ml, respectively. The flesh and peel extracts cause apoptosis in HeLa cells. Cell cycle arrest in sub-G1 (M) and cell cycle inhibition in G0-G1 are caused by the C. tuberosus peel extract. According to this study, the CFEE and CPEEs have the potential to be a source of natural antioxidants and antiproliferation of cervical cancer.","PeriodicalId":15032,"journal":{"name":"Journal of Applied Biology and Biotechnology","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71218285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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